AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022060.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022060.01.T01 MTR_4g081540 100.000 114 0 0 1 114 673 786 5.90e-76 239
MsG0480022060.01.T01 MTR_4g099200 35.088 114 72 2 1 113 573 685 1.86e-19 82.4
MsG0480022060.01.T01 MTR_5g044930 37.931 116 64 4 1 113 552 662 5.23e-18 78.6
MsG0480022060.01.T01 MTR_5g045620 37.931 116 64 4 1 113 548 658 7.20e-17 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022060.01.T01 AT1G03100 79.825 114 22 1 1 113 679 792 3.54e-58 191
MsG0480022060.01.T01 AT4G17616 33.043 115 75 2 1 114 561 674 8.49e-17 75.1
MsG0480022060.01.T01 AT1G69290 35.043 117 67 4 1 113 545 656 2.80e-16 73.6

Find 28 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGTTCCTGATCAAACTTTA+TGG 0.205294 4:-67552589 None:intergenic
TGTGATTCTGTCTGAAATTT+CGG 0.259129 4:-67552768 None:intergenic
CATCGTTGCCACAACTTCAT+TGG 0.300998 4:-67552661 None:intergenic
TCACTGGGTATGCTGCCATT+GGG 0.344721 4:+67552505 MsG0480022060.01.T01:CDS
GTCACTGGGTATGCTGCCAT+TGG 0.367999 4:+67552504 MsG0480022060.01.T01:CDS
CAGAATCACAATTAAACAAA+AGG 0.410643 4:+67552781 MsG0480022060.01.T01:CDS
GTTGTGGCAACGATGGAAAA+AGG 0.431562 4:+67552669 MsG0480022060.01.T01:CDS
CACTGGGTATGCTGCCATTG+GGG 0.438082 4:+67552506 MsG0480022060.01.T01:CDS
ACTTTCCATTCTATGGTCAC+TGG 0.446895 4:+67552489 MsG0480022061.01.T01:CDS
GTCCTCCATAAAGTTTGATC+AGG 0.465447 4:+67552584 MsG0480022060.01.T01:CDS
ACTGGGTATGCTGCCATTGG+GGG 0.478931 4:+67552507 MsG0480022060.01.T01:CDS
AGGTGGATCGGCATGATTTG+AGG 0.481005 4:+67552825 MsG0480022060.01.T01:CDS
TTGTATACATTTGTGAGGGG+TGG 0.490566 4:+67552624 MsG0480022060.01.T01:CDS
CTTTCCATTCTATGGTCACT+GGG 0.495599 4:+67552490 MsG0480022061.01.T01:CDS
CTGTCTTGTATACATTTGTG+AGG 0.510919 4:+67552619 MsG0480022060.01.T01:CDS
AGAATCACAATTAAACAAAA+GGG 0.523544 4:+67552782 MsG0480022060.01.T01:CDS
TGTCTTGTATACATTTGTGA+GGG 0.532711 4:+67552620 MsG0480022060.01.T01:CDS
TCTGGAGGTAACAGAGCTGT+GGG 0.538692 4:+67552536 MsG0480022060.01.T01:CDS
CTGGGTATGCTGCCATTGGG+GGG 0.579929 4:+67552508 MsG0480022060.01.T01:CDS
ATCTGGAGGTAACAGAGCTG+TGG 0.583924 4:+67552535 MsG0480022060.01.T01:CDS
CATCCGTGCCAATGAAGTTG+TGG 0.590357 4:+67552653 MsG0480022060.01.T01:CDS
TTGCCACAACTTCATTGGCA+CGG 0.600337 4:-67552656 None:intergenic
GTCTTGTATACATTTGTGAG+GGG 0.602463 4:+67552621 MsG0480022060.01.T01:CDS
CAAAACACTTTACAAAGGCA+AGG 0.611151 4:+67552743 MsG0480022060.01.T01:CDS
TTCCTGATCAAACTTTATGG+AGG 0.630008 4:-67552586 None:intergenic
CAATGAAGTTGTGGCAACGA+TGG 0.638976 4:+67552662 MsG0480022060.01.T01:CDS
TATCACAAAACACTTTACAA+AGG 0.639956 4:+67552738 MsG0480022060.01.T01:CDS
CTGGAGGTAACAGAGCTGTG+GGG 0.758688 4:+67552537 MsG0480022060.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAATCACAATTAAACAAAA+GGG + Chr4:67552782-67552801 MsG0480022060.01.T01:CDS 20.0%
! CAGAATCACAATTAAACAAA+AGG + Chr4:67552781-67552800 MsG0480022060.01.T01:CDS 25.0%
! TATCACAAAACACTTTACAA+AGG + Chr4:67552738-67552757 MsG0480022060.01.T01:CDS 25.0%
AAGTTCCTGATCAAACTTTA+TGG - Chr4:67552592-67552611 None:intergenic 30.0%
TGTCTTGTATACATTTGTGA+GGG + Chr4:67552620-67552639 MsG0480022060.01.T01:CDS 30.0%
! TGTGATTCTGTCTGAAATTT+CGG - Chr4:67552771-67552790 None:intergenic 30.0%
CAAAACACTTTACAAAGGCA+AGG + Chr4:67552743-67552762 MsG0480022060.01.T01:CDS 35.0%
CTGTCTTGTATACATTTGTG+AGG + Chr4:67552619-67552638 MsG0480022060.01.T01:CDS 35.0%
GTCTTGTATACATTTGTGAG+GGG + Chr4:67552621-67552640 MsG0480022060.01.T01:CDS 35.0%
TTCCTGATCAAACTTTATGG+AGG - Chr4:67552589-67552608 None:intergenic 35.0%
!! CTGACTTTTAAAAGGTGGAT+CGG + Chr4:67552813-67552832 MsG0480022060.01.T01:CDS 35.0%
!! GATCCACCTTTTAAAAGTCA+GGG - Chr4:67552814-67552833 None:intergenic 35.0%
GTCCTCCATAAAGTTTGATC+AGG + Chr4:67552584-67552603 MsG0480022060.01.T01:CDS 40.0%
TTGTATACATTTGTGAGGGG+TGG + Chr4:67552624-67552643 MsG0480022060.01.T01:CDS 40.0%
! AAGCAGCCCTGACTTTTAAA+AGG + Chr4:67552805-67552824 MsG0480022060.01.T01:CDS 40.0%
!! CGATCCACCTTTTAAAAGTC+AGG - Chr4:67552815-67552834 None:intergenic 40.0%
CAATGAAGTTGTGGCAACGA+TGG + Chr4:67552662-67552681 MsG0480022060.01.T01:CDS 45.0%
CATCGTTGCCACAACTTCAT+TGG - Chr4:67552664-67552683 None:intergenic 45.0%
GTTGTGGCAACGATGGAAAA+AGG + Chr4:67552669-67552688 MsG0480022060.01.T01:CDS 45.0%
TTGCCACAACTTCATTGGCA+CGG - Chr4:67552659-67552678 None:intergenic 45.0%
!! CAGCCCTGACTTTTAAAAGG+TGG + Chr4:67552808-67552827 MsG0480022060.01.T01:CDS 45.0%
ATCTGGAGGTAACAGAGCTG+TGG + Chr4:67552535-67552554 MsG0480022060.01.T01:CDS 50.0%
CATCCGTGCCAATGAAGTTG+TGG + Chr4:67552653-67552672 MsG0480022060.01.T01:CDS 50.0%
CATTGGGGGGAAATATCTGG+AGG + Chr4:67552521-67552540 MsG0480022060.01.T01:CDS 50.0%
CTCCAGATATTTCCCCCCAA+TGG - Chr4:67552523-67552542 None:intergenic 50.0%
TCACTGGGTATGCTGCCATT+GGG + Chr4:67552505-67552524 MsG0480022060.01.T01:CDS 50.0%
TCTGGAGGTAACAGAGCTGT+GGG + Chr4:67552536-67552555 MsG0480022060.01.T01:CDS 50.0%
TGCCATTGGGGGGAAATATC+TGG + Chr4:67552518-67552537 MsG0480022060.01.T01:CDS 50.0%
ACTGGGTATGCTGCCATTGG+GGG + Chr4:67552507-67552526 MsG0480022060.01.T01:CDS 55.0%
CACTGGGTATGCTGCCATTG+GGG + Chr4:67552506-67552525 MsG0480022060.01.T01:CDS 55.0%
CTGGAGGTAACAGAGCTGTG+GGG + Chr4:67552537-67552556 MsG0480022060.01.T01:CDS 55.0%
GTCACTGGGTATGCTGCCAT+TGG + Chr4:67552504-67552523 MsG0480022060.01.T01:CDS 55.0%
CTGGGTATGCTGCCATTGGG+GGG + Chr4:67552508-67552527 MsG0480022060.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 67552501 67552845 67552501 ID=MsG0480022060.01;Name=MsG0480022060.01
Chr4 mRNA 67552501 67552845 67552501 ID=MsG0480022060.01.T01;Parent=MsG0480022060.01;Name=MsG0480022060.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|114
Chr4 exon 67552501 67552845 67552501 ID=MsG0480022060.01.T01:exon:4966;Parent=MsG0480022060.01.T01
Chr4 CDS 67552501 67552845 67552501 ID=MsG0480022060.01.T01:cds;Parent=MsG0480022060.01.T01
Gene Sequence

>MsG0480022060.01.T01

ATGGTCACTGGGTATGCTGCCATTGGGGGGAAATATCTGGAGGTAACAGAGCTGTGGGGTGAGATGAAATCACTTGCTTCTAAGTCCTCCATAAAGTTTGATCAGGAACTTCTAGATTCTGTCTTGTATACATTTGTGAGGGGTGGATTCTTCATCCGTGCCAATGAAGTTGTGGCAACGATGGAAAAAGGAAAAATGTTTATTGACAAGTACAAATACAGAATGCTATTCTTGAAATATCACAAAACACTTTACAAAGGCAAGGCTCCGAAATTTCAGACAGAATCACAATTAAACAAAAGGGAAGCAGCCCTGACTTTTAAAAGGTGGATCGGCATGATTTGA

Protein sequence

>MsG0480022060.01.T01

MVTGYAAIGGKYLEVTELWGEMKSLASKSSIKFDQELLDSVLYTFVRGGFFIRANEVVATMEKGKMFIDKYKYRMLFLKYHKTLYKGKAPKFQTESQLNKREAALTFKRWIGMI*