Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030320.01.T01 | XP_013450735.1 | 93.081 | 607 | 34 | 3 | 32 | 635 | 30 | 631 | 0 | 1061 |
| MsG0680030320.01.T02 | XP_013450735.1 | 93.081 | 607 | 34 | 3 | 32 | 635 | 30 | 631 | 0 | 1061 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030320.01.T01 | A0A072U6I9 | 93.081 | 607 | 34 | 3 | 32 | 635 | 30 | 631 | 0.0 | 1061 |
| MsG0680030320.01.T02 | A0A072U6I9 | 93.081 | 607 | 34 | 3 | 32 | 635 | 30 | 631 | 0.0 | 1061 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047791.01 | MsG0680030320.01 | 0.805283 | 1.438724e-49 | 7.581041e-47 |
| MsG0080047944.01 | MsG0680030320.01 | 0.803507 | 3.372655e-49 | 1.697924e-46 |
| MsG0080048683.01 | MsG0680030320.01 | 0.803974 | 2.697738e-49 | 1.374540e-46 |
| MsG0080048785.01 | MsG0680030320.01 | 0.809704 | 1.659232e-50 | 9.810911e-48 |
| MsG0080048817.01 | MsG0680030320.01 | 0.815757 | 7.850334e-52 | 5.460308e-49 |
| MsG0080048856.01 | MsG0680030320.01 | 0.803133 | 4.030889e-49 | 2.009779e-46 |
| MsG0180000139.01 | MsG0680030320.01 | 0.803134 | 4.029859e-49 | 2.009280e-46 |
| MsG0180000209.01 | MsG0680030320.01 | 0.859378 | 4.310923e-63 | 1.136307e-59 |
| MsG0180000210.01 | MsG0680030320.01 | 0.827018 | 1.971915e-54 | 1.880440e-51 |
| MsG0180000470.01 | MsG0680030320.01 | 0.818776 | 1.642632e-52 | 1.241135e-49 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030320.01.T01 | MTR_6g004480 | 93.081 | 607 | 34 | 3 | 32 | 635 | 30 | 631 | 0.0 | 1061 |
| MsG0680030320.01.T01 | MTR_1g050760 | 50.000 | 174 | 85 | 1 | 112 | 283 | 1 | 174 | 6.16e-49 | 174 |
| MsG0680030320.01.T01 | MTR_1g050760 | 50.000 | 174 | 85 | 1 | 112 | 283 | 116 | 289 | 4.14e-47 | 172 |
| MsG0680030320.01.T01 | MTR_4g120880 | 48.521 | 169 | 87 | 0 | 115 | 283 | 161 | 329 | 4.09e-41 | 153 |
| MsG0680030320.01.T01 | MTR_4g067300 | 44.970 | 169 | 93 | 0 | 115 | 283 | 134 | 302 | 1.02e-39 | 151 |
| MsG0680030320.01.T01 | MTR_4g120880 | 48.299 | 147 | 76 | 0 | 115 | 261 | 161 | 307 | 1.53e-34 | 134 |
| MsG0680030320.01.T01 | MTR_6g084410 | 32.701 | 211 | 128 | 5 | 79 | 286 | 557 | 756 | 5.64e-23 | 104 |
| MsG0680030320.01.T01 | MTR_1g475240 | 31.925 | 213 | 127 | 6 | 79 | 286 | 566 | 765 | 6.34e-23 | 104 |
| MsG0680030320.01.T01 | MTR_1g075190 | 31.925 | 213 | 127 | 6 | 79 | 286 | 566 | 765 | 6.34e-23 | 104 |
| MsG0680030320.01.T01 | MTR_6g008410 | 35.593 | 177 | 106 | 3 | 116 | 286 | 518 | 692 | 1.66e-21 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030320.01.T01 | AT3G27550 | 80.303 | 330 | 60 | 2 | 63 | 387 | 1 | 330 | 4.25e-163 | 476 |
| MsG0680030320.01.T01 | AT3G27550 | 80.303 | 330 | 60 | 2 | 63 | 387 | 1 | 330 | 4.25e-163 | 476 |
| MsG0680030320.01.T01 | AT3G27550 | 80.303 | 330 | 60 | 2 | 63 | 387 | 57 | 386 | 3.94e-162 | 476 |
| MsG0680030320.01.T01 | AT3G25440 | 45.918 | 196 | 104 | 1 | 98 | 291 | 57 | 252 | 3.25e-47 | 171 |
| MsG0680030320.01.T01 | AT3G25440 | 45.918 | 196 | 104 | 1 | 98 | 291 | 121 | 316 | 2.22e-46 | 170 |
| MsG0680030320.01.T01 | AT2G28480 | 46.857 | 175 | 93 | 0 | 109 | 283 | 119 | 293 | 1.30e-43 | 160 |
| MsG0680030320.01.T01 | AT3G25440 | 47.826 | 161 | 82 | 1 | 133 | 291 | 1 | 161 | 6.01e-42 | 154 |
| MsG0680030320.01.T01 | AT3G25440 | 47.826 | 161 | 82 | 1 | 133 | 291 | 1 | 161 | 6.01e-42 | 154 |
| MsG0680030320.01.T01 | AT3G25440 | 47.826 | 161 | 82 | 1 | 133 | 291 | 1 | 161 | 6.01e-42 | 154 |
| MsG0680030320.01.T01 | AT4G13070 | 45.128 | 195 | 97 | 3 | 80 | 270 | 111 | 299 | 2.98e-40 | 150 |
Find 133 sgRNAs with CRISPR-Local
Find 443 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACTATTACGATATTGAG+CGG | 0.607700 | 6:-584664 | None:intergenic |
| AAACATAAAGAAAATAGAAC+AGG | 0.489664 | 6:+583831 | MsG0680030320.01.T01:CDS |
| AAAGGAAGCTGATGAAGCTT+GGG | 0.481393 | 6:+584884 | MsG0680030320.01.T01:CDS |
| AAATAGAACAGGACCTGCGG+CGG | 0.657187 | 6:+583842 | MsG0680030320.01.T01:CDS |
| AAATGATGACTATGATGGTG+AGG | 0.683700 | 6:+584188 | MsG0680030320.01.T01:CDS |
| AACCAATCAACAATTTCTTC+AGG | 0.430070 | 6:-585151 | None:intergenic |
| AATAATACAAGTATTGGCAA+TGG | 0.589479 | 6:+584429 | MsG0680030320.01.T01:CDS |
| AATAGAACCTAATGATGGTT+TGG | 0.420274 | 6:+583990 | MsG0680030320.01.T01:CDS |
| AATAGAACCTTGAGGAGTTG+AGG | 0.579296 | 6:-585249 | None:intergenic |
| AATGACTCGGTACATTTGAG+AGG | 0.617243 | 6:+584582 | MsG0680030320.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATAGACATGGAACTGTT+AGG | + | Chr6:581323-581342 | MsG0680030320.01.T01:intron | 30.0% | |
| AAAGAATTTGGTCCTAGTTA+TGG | + | Chr6:583697-583716 | MsG0680030320.01.T01:intron | 30.0% | |
| AAAGCCTTCTTAAACACTTT+AGG | + | Chr6:581079-581098 | MsG0680030320.01.T01:intron | 30.0% | |
| AACAAGAATTATGTTCCAGT+TGG | + | Chr6:582998-583017 | MsG0680030320.01.T01:CDS | 30.0% | |
| AACCAATCAACAATTTCTTC+AGG | - | Chr6:585154-585173 | None:intergenic | 30.0% | |
| AAGAGTCTGGTAAAACATTA+AGG | + | Chr6:580802-580821 | MsG0680030320.01.T01:CDS | 30.0% | |
| AAGTTGAAAGAGAGATGAAA+TGG | - | Chr6:580343-580362 | None:intergenic | 30.0% | |
| AATAGAACCTAATGATGGTT+TGG | + | Chr6:583990-584009 | MsG0680030320.01.T01:CDS | 30.0% | |
| ACAAACTTGTATGACAATTC+TGG | + | Chr6:582471-582490 | MsG0680030320.01.T01:intron | 30.0% | |
| ACATGTGGCAAAGATTTAAA+TGG | + | Chr6:580063-580082 | MsG0680030320.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 579429 | 585770 | 579429 | ID=MsG0680030320.01;Name=MsG0680030320.01 |
| Chr6 | mRNA | 579429 | 585770 | 579429 | ID=MsG0680030320.01.T01;Parent=MsG0680030320.01;Name=MsG0680030320.01.T01;_AED=0.48;_eAED=0.48;_QI=0|0.75|0.8|0.8|0.75|0.6|5|307|635 |
| Chr6 | exon | 583784 | 585204 | 583784 | ID=MsG0680030320.01.T01:exon:2143;Parent=MsG0680030320.01.T01 |
| Chr6 | exon | 585751 | 585770 | 585751 | ID=MsG0680030320.01.T01:exon:2144;Parent=MsG0680030320.01.T01 |
| Chr6 | CDS | 579429 | 579517 | 579429 | ID=MsG0680030320.01.T01:cds;Parent=MsG0680030320.01.T01 |
| Chr6 | CDS | 580617 | 580899 | 580617 | ID=MsG0680030320.01.T01:cds;Parent=MsG0680030320.01.T01 |
| Chr6 | CDS | 582866 | 583267 | 582866 | ID=MsG0680030320.01.T01:cds;Parent=MsG0680030320.01.T01 |
| Chr6 | CDS | 583784 | 584917 | 583784 | ID=MsG0680030320.01.T01:cds;Parent=MsG0680030320.01.T01 |
| Chr6 | three_prime_UTR | 584918 | 585204 | 584918 | ID=MsG0680030320.01.T01:three_prime_utr;Parent=MsG0680030320.01.T01 |
| Chr6 | three_prime_UTR | 585751 | 585770 | 585751 | ID=MsG0680030320.01.T01:three_prime_utr;Parent=MsG0680030320.01.T01 |
| Chr6 | mRNA | 579429 | 585366 | 579429 | ID=MsG0680030320.01.T02;Parent=MsG0680030320.01;Name=MsG0680030320.01.T02;_AED=0.48;_eAED=0.48;_QI=0|0.66|0.75|0.75|1|1|4|449|635 |
| Chr6 | exon | 583784 | 585366 | 583784 | ID=MsG0680030320.01.T02:exon:2145;Parent=MsG0680030320.01.T02 |
| Chr6 | CDS | 579429 | 579517 | 579429 | ID=MsG0680030320.01.T02:cds;Parent=MsG0680030320.01.T02 |
| Chr6 | CDS | 580617 | 580899 | 580617 | ID=MsG0680030320.01.T02:cds;Parent=MsG0680030320.01.T02 |
| Chr6 | CDS | 582866 | 583267 | 582866 | ID=MsG0680030320.01.T02:cds;Parent=MsG0680030320.01.T02 |
| Chr6 | CDS | 583784 | 584917 | 583784 | ID=MsG0680030320.01.T02:cds;Parent=MsG0680030320.01.T02 |
| Chr6 | three_prime_UTR | 584918 | 585366 | 584918 | ID=MsG0680030320.01.T02:three_prime_utr;Parent=MsG0680030320.01.T02 |
| Gene Sequence |
>MsG0680030320.01.T01 ATGATTGGATATCGTTTTTACAATAGTGGTTTCATGAGGGAGTCTGTTTTAGGTGGTGACGTGTATTTTGGTGGATGTGGTCCTCATGGAAATGTTGTGCCTTTAGGACAAGGAATAGAGCAAAGTAAAACTGATGATAAAGTTGAAGCGTACCATAACGATAGCTTTTATTGTTATGTTAAACCGTTGAATCTTTTGAATGAATCCTTGTGTCGGGGAATGTCAACGATGAGAGGAAAGAGTATGCGAAGCAAGGTGGAAAGAAGAATGCAGAAAGAGTCTGGTAAAACATTAAGGGAAATTCGAAAAGCTAAAAAAATTACGAAGAAACTTATGACTGACGAGGAGCGGCTTGTTTACAATCTCAAAAGAGCAAAGAAGAAAATTGCATTGCTACTACAAAAGCTTAAGAAATATGAGCTACCGGAGCTGCCTCCACCACGGCATGATCCAGAGCTCTTTACACCCGAACAGCTTCAGGCGTATAAAAAAATTGGATTCAAGAACAAGAATTATGTTCCAGTTGGTGTTCGTGGAGTATTTGGAGGAGTTGTGCAAAATATGCATCTCCATTGGAAGTTTCATGAGACTGTGCAAGTTTGTTGTGACAACTTCCCCAAGGAAAGAATTAAAGAAATGGCTTCAATGCTTGCAAGACTGAGTGGTGGGATCGTGTTAAACGTTCATAATGTGAAAACAATCATCATGTTCCGTGGTAGAAATTACCGCCAACCCAAAAATTTGATACCTATTAATACCCTTACAAAGAGGAAGGCCCTATTCAAGGCCAGATTTGAGCAAGCTCTTGAATCTCAAAAGTTAAACATAAAGAAAATAGAACAGGACCTGCGGCGGATGGGAGTAAATCCTGAGGATCCAACTGCAATGGCCAGCATCCAAAGAGTGGCTTCCACTTTCTTCAATGCCATTGACAAGAAAGATGGAAGTCCTTATGTTAACCGTGGAGACAAGCCGTCAGTAATAGAACCTAATGATGGTTTGGAGGAATCAGATCCTGCTGAACAGGAAGAGCTAGATCGGTTCATCGCTGATATAGAGGATGCCGCCGATAAAGAATATGAAGCTGAAGAGGCCAAAGAAAAGGAAGAGTTTGGAAGACTTAGATATTGGAACAGAGAAGAGTATGGTGGAAGATTCAGAAGATTGAACGATTCTAGAAATGATGACTATGATGGTGAGGTTAGAGGCTCAAGAGTTCGGCAGAATATACATCCCAAGCATCGGACTGCCGATAGCGACGATGAAGAGAATGCTTATTCTAACAATGACGACGACGACGACGATGGTGAGTGGCATCATGGTAATATTGCCGATGACAGTGATGTTGACAGCAATTTTGATGGCTCTGATGAAGCTAGAGGTAGGTTCAAGGAAAGTAGGGGGAGAAGAGAGAGGATAAATAATACAAGTATTGGCAATGGTCAAGTTAATGGTGGTCAGAGCAGACACGGTGAAGCTAAATTTAGGACGAATATGGGTGTACAAGATTTTGAATCAGAAGATATACTTAGCGAAGTTGAAAATGCAATGTGGGAGTCGGATTTGGAGGACAATGACTCGGTACATTTGAGAGGAGTCAATAGCAGTAACTATAAGAGCAGCAGCAGCGATGATGAGGGTCAGATTGATGATTCCGCTCAATATCGTAATAGTTTTAATGCACCAAAGAGTTTGAGAGGCAGGGAAGCAGGAATTATCCGCACTAAATCCGTGTCCATGGATGCCTTTAGCGGTTTGGAAGACTGGATGTCCCAATCAGAAGGTGAGGAGGACTCAAGAGGTGGTGATAAGGACTACCCTACAAGTCAAAATAGTAAGGTGGTCAAAAGTGATATCCATAAGAAGAGATCACCAAAGGAAGCTGATGAAGCTTGGGACAGTGACTAG >MsG0680030320.01.T02 ATGATTGGATATCGTTTTTACAATAGTGGTTTCATGAGGGAGTCTGTTTTAGGTGGTGACGTGTATTTTGGTGGATGTGGTCCTCATGGAAATGTTGTGCCTTTAGGACAAGGAATAGAGCAAAGTAAAACTGATGATAAAGTTGAAGCGTACCATAACGATAGCTTTTATTGTTATGTTAAACCGTTGAATCTTTTGAATGAATCCTTGTGTCGGGGAATGTCAACGATGAGAGGAAAGAGTATGCGAAGCAAGGTGGAAAGAAGAATGCAGAAAGAGTCTGGTAAAACATTAAGGGAAATTCGAAAAGCTAAAAAAATTACGAAGAAACTTATGACTGACGAGGAGCGGCTTGTTTACAATCTCAAAAGAGCAAAGAAGAAAATTGCATTGCTACTACAAAAGCTTAAGAAATATGAGCTACCGGAGCTGCCTCCACCACGGCATGATCCAGAGCTCTTTACACCCGAACAGCTTCAGGCGTATAAAAAAATTGGATTCAAGAACAAGAATTATGTTCCAGTTGGTGTTCGTGGAGTATTTGGAGGAGTTGTGCAAAATATGCATCTCCATTGGAAGTTTCATGAGACTGTGCAAGTTTGTTGTGACAACTTCCCCAAGGAAAGAATTAAAGAAATGGCTTCAATGCTTGCAAGACTGAGTGGTGGGATCGTGTTAAACGTTCATAATGTGAAAACAATCATCATGTTCCGTGGTAGAAATTACCGCCAACCCAAAAATTTGATACCTATTAATACCCTTACAAAGAGGAAGGCCCTATTCAAGGCCAGATTTGAGCAAGCTCTTGAATCTCAAAAGTTAAACATAAAGAAAATAGAACAGGACCTGCGGCGGATGGGAGTAAATCCTGAGGATCCAACTGCAATGGCCAGCATCCAAAGAGTGGCTTCCACTTTCTTCAATGCCATTGACAAGAAAGATGGAAGTCCTTATGTTAACCGTGGAGACAAGCCGTCAGTAATAGAACCTAATGATGGTTTGGAGGAATCAGATCCTGCTGAACAGGAAGAGCTAGATCGGTTCATCGCTGATATAGAGGATGCCGCCGATAAAGAATATGAAGCTGAAGAGGCCAAAGAAAAGGAAGAGTTTGGAAGACTTAGATATTGGAACAGAGAAGAGTATGGTGGAAGATTCAGAAGATTGAACGATTCTAGAAATGATGACTATGATGGTGAGGTTAGAGGCTCAAGAGTTCGGCAGAATATACATCCCAAGCATCGGACTGCCGATAGCGACGATGAAGAGAATGCTTATTCTAACAATGACGACGACGACGACGATGGTGAGTGGCATCATGGTAATATTGCCGATGACAGTGATGTTGACAGCAATTTTGATGGCTCTGATGAAGCTAGAGGTAGGTTCAAGGAAAGTAGGGGGAGAAGAGAGAGGATAAATAATACAAGTATTGGCAATGGTCAAGTTAATGGTGGTCAGAGCAGACACGGTGAAGCTAAATTTAGGACGAATATGGGTGTACAAGATTTTGAATCAGAAGATATACTTAGCGAAGTTGAAAATGCAATGTGGGAGTCGGATTTGGAGGACAATGACTCGGTACATTTGAGAGGAGTCAATAGCAGTAACTATAAGAGCAGCAGCAGCGATGATGAGGGTCAGATTGATGATTCCGCTCAATATCGTAATAGTTTTAATGCACCAAAGAGTTTGAGAGGCAGGGAAGCAGGAATTATCCGCACTAAATCCGTGTCCATGGATGCCTTTAGCGGTTTGGAAGACTGGATGTCCCAATCAGAAGGTGAGGAGGACTCAAGAGGTGGTGATAAGGACTACCCTACAAGTCAAAATAGTAAGGTGGTCAAAAGTGATATCCATAAGAAGAGATCACCAAAGGAAGCTGATGAAGCTTGGGACAGTGACTAG |
| Protein sequence |
>MsG0680030320.01.T01 MIGYRFYNSGFMRESVLGGDVYFGGCGPHGNVVPLGQGIEQSKTDDKVEAYHNDSFYCYVKPLNLLNESLCRGMSTMRGKSMRSKVERRMQKESGKTLREIRKAKKITKKLMTDEERLVYNLKRAKKKIALLLQKLKKYELPELPPPRHDPELFTPEQLQAYKKIGFKNKNYVPVGVRGVFGGVVQNMHLHWKFHETVQVCCDNFPKERIKEMASMLARLSGGIVLNVHNVKTIIMFRGRNYRQPKNLIPINTLTKRKALFKARFEQALESQKLNIKKIEQDLRRMGVNPEDPTAMASIQRVASTFFNAIDKKDGSPYVNRGDKPSVIEPNDGLEESDPAEQEELDRFIADIEDAADKEYEAEEAKEKEEFGRLRYWNREEYGGRFRRLNDSRNDDYDGEVRGSRVRQNIHPKHRTADSDDEENAYSNNDDDDDDGEWHHGNIADDSDVDSNFDGSDEARGRFKESRGRRERINNTSIGNGQVNGGQSRHGEAKFRTNMGVQDFESEDILSEVENAMWESDLEDNDSVHLRGVNSSNYKSSSSDDEGQIDDSAQYRNSFNAPKSLRGREAGIIRTKSVSMDAFSGLEDWMSQSEGEEDSRGGDKDYPTSQNSKVVKSDIHKKRSPKEADEAWDSD* >MsG0680030320.01.T02 MIGYRFYNSGFMRESVLGGDVYFGGCGPHGNVVPLGQGIEQSKTDDKVEAYHNDSFYCYVKPLNLLNESLCRGMSTMRGKSMRSKVERRMQKESGKTLREIRKAKKITKKLMTDEERLVYNLKRAKKKIALLLQKLKKYELPELPPPRHDPELFTPEQLQAYKKIGFKNKNYVPVGVRGVFGGVVQNMHLHWKFHETVQVCCDNFPKERIKEMASMLARLSGGIVLNVHNVKTIIMFRGRNYRQPKNLIPINTLTKRKALFKARFEQALESQKLNIKKIEQDLRRMGVNPEDPTAMASIQRVASTFFNAIDKKDGSPYVNRGDKPSVIEPNDGLEESDPAEQEELDRFIADIEDAADKEYEAEEAKEKEEFGRLRYWNREEYGGRFRRLNDSRNDDYDGEVRGSRVRQNIHPKHRTADSDDEENAYSNNDDDDDDGEWHHGNIADDSDVDSNFDGSDEARGRFKESRGRRERINNTSIGNGQVNGGQSRHGEAKFRTNMGVQDFESEDILSEVENAMWESDLEDNDSVHLRGVNSSNYKSSSSDDEGQIDDSAQYRNSFNAPKSLRGREAGIIRTKSVSMDAFSGLEDWMSQSEGEEDSRGGDKDYPTSQNSKVVKSDIHKKRSPKEADEAWDSD* |
