AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022181.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022181.01.T01 MTR_4g083780 71.875 64 11 2 2 64 3 60 4.53e-20 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 23 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAGGTGGTTTGGGCCTTTA+TGG 0.068346 4:+69361254 MsG0480022181.01.T01:CDS
GGACACTTTAGAGGGAAAAT+TGG 0.262444 4:+69361194 MsG0480022181.01.T01:CDS
TTGGGCCTAATGTGGAATTT+TGG 0.281950 4:+69361219 MsG0480022181.01.T01:CDS
ATTCTCACTCCAGGTGGTTT+GGG 0.282275 4:+69361245 MsG0480022181.01.T01:CDS
ACTCTTTCTGCTGACGCTTC+TGG 0.300055 4:+69359913 MsG0480022181.01.T01:CDS
GATTCTCACTCCAGGTGGTT+TGG 0.305473 4:+69361244 MsG0480022181.01.T01:CDS
GAGAGACTCGAGAGTGTAAA+AGG 0.333923 4:-69359943 None:intergenic
TTGTAAAAGACCCAATAAAA+AGG 0.342474 4:+69361284 MsG0480022181.01.T01:CDS
GATTGAAATGGACACTTTAG+AGG 0.380042 4:+69361185 MsG0480022181.01.T01:CDS
TTACTATAATTCCTAACTTC+AGG 0.386426 4:+69360390 MsG0480022181.01.T01:CDS
TATTTAGGACATGATTGAAA+TGG 0.412335 4:+69361173 MsG0480022181.01.T01:intron
ACATATACCTTCCTGAAGTT+AGG 0.445979 4:-69360401 None:intergenic
ATCTACCAAAATTCCACATT+AGG 0.460649 4:-69361224 None:intergenic
TTAGAGGGAAAATTGGAGTT+GGG 0.461347 4:+69361201 MsG0480022181.01.T01:CDS
CCATAAAGGCCCAAACCACC+TGG 0.504845 4:-69361254 None:intergenic
TTTAGAGGGAAAATTGGAGT+TGG 0.516310 4:+69361200 MsG0480022181.01.T01:CDS
GCAAATCGTTGACGTTGGAT+CGG 0.580211 4:+69359882 MsG0480022181.01.T01:CDS
AATTGGAGTTGGGCCTAATG+TGG 0.592160 4:+69361211 MsG0480022181.01.T01:CDS
ATTGAAATGGACACTTTAGA+GGG 0.592478 4:+69361186 MsG0480022181.01.T01:CDS
TCGTAGCAAAATCAAAACAA+TGG 0.601320 4:+69359846 MsG0480022181.01.T01:exon
TGGTAGATTCTCACTCCAGG+TGG 0.605046 4:+69361239 MsG0480022181.01.T01:CDS
TATAATTCCTAACTTCAGGA+AGG 0.606546 4:+69360394 MsG0480022181.01.T01:CDS
AGAGAGCAAATCGTTGACGT+TGG 0.610890 4:+69359877 MsG0480022181.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAAAGATTAAAATAAAA+AGG - Chr4:69361154-69361173 None:intergenic 10.0%
!!! ATTTTAATCTTTTGTTATTT+AGG + Chr4:69361158-69361177 MsG0480022181.01.T01:intron 10.0%
!! AAAAAGTAAGAAGAAAAATT+AGG - Chr4:69360132-69360151 None:intergenic 15.0%
!! AAAAATAATATATCAGCATA+AGG - Chr4:69360562-69360581 None:intergenic 15.0%
!! AAAAGAGTAAAAAAATAAAG+AGG - Chr4:69360064-69360083 None:intergenic 15.0%
!! AAAAGATTAAAATAAAAAGG+TGG - Chr4:69361151-69361170 None:intergenic 15.0%
!! AAAAGTAAGAAGAAAAATTA+GGG - Chr4:69360131-69360150 None:intergenic 15.0%
!! AATAAAAAGGAAAAACAAAA+TGG + Chr4:69361297-69361316 MsG0480022181.01.T01:CDS 15.0%
!! ACTAATTATCACTAATTAAT+TGG - Chr4:69360460-69360479 None:intergenic 15.0%
!! CTAATTATCACTAATTAATT+GGG - Chr4:69360459-69360478 None:intergenic 15.0%
!! TAATTATCACTAATTAATTG+GGG - Chr4:69360458-69360477 None:intergenic 15.0%
!!! ATTTTGTTTTTCCTTTTTAT+TGG - Chr4:69361298-69361317 None:intergenic 15.0%
!!! TAAAAAGATTTAAAGTTGAT+GGG + Chr4:69361061-69361080 MsG0480022181.01.T01:intron 15.0%
!!! TGATATTTGTTAATTAATTC+CGG + Chr4:69360790-69360809 MsG0480022181.01.T01:intron 15.0%
!!! TTAAAAAGATTTAAAGTTGA+TGG + Chr4:69361060-69361079 MsG0480022181.01.T01:intron 15.0%
!!! TTTACAATTTTTTTCCATAA+AGG - Chr4:69361271-69361290 None:intergenic 15.0%
!!! TTTTGTTTTTCCTTTTTATT+GGG - Chr4:69361297-69361316 None:intergenic 15.0%
!! TAAATTCTGTTATGCTATAA+CGG + Chr4:69360954-69360973 MsG0480022181.01.T01:intron 20.0%
!! TTGTACAAAATATTGTCAAA+TGG - Chr4:69360902-69360921 None:intergenic 20.0%
!!! TTATTGTTTCTATGTGAATT+TGG + Chr4:69360225-69360244 MsG0480022181.01.T01:intron 20.0%
!!! TTGGAGTTATTGTATTTTTA+GGG + Chr4:69360244-69360263 MsG0480022181.01.T01:intron 20.0%
!!! TTTGGAGTTATTGTATTTTT+AGG + Chr4:69360243-69360262 MsG0480022181.01.T01:intron 20.0%
! AAACTTAGGTAATTGGTATT+GGG + Chr4:69360492-69360511 MsG0480022181.01.T01:intron 25.0%
! AATGATGTATCAAGAATCAT+GGG - Chr4:69361091-69361110 None:intergenic 25.0%
! TGAATGCATTCATGTAAAAT+TGG + Chr4:69360847-69360866 MsG0480022181.01.T01:intron 25.0%
! TGCAGTAAAATCAATTTGAA+TGG - Chr4:69360629-69360648 None:intergenic 25.0%
! TTACTATAATTCCTAACTTC+AGG + Chr4:69360390-69360409 MsG0480022181.01.T01:CDS 25.0%
! TTGTAAAAGACCCAATAAAA+AGG + Chr4:69361284-69361303 MsG0480022181.01.T01:CDS 25.0%
! TTTACAGCTTCATCTAATTA+GGG + Chr4:69360273-69360292 MsG0480022181.01.T01:intron 25.0%
!! AAATTTCTCTCATGTTTTTC+AGG + Chr4:69360349-69360368 MsG0480022181.01.T01:intron 25.0%
!! AATTTCTCTCATGTTTTTCA+GGG + Chr4:69360350-69360369 MsG0480022181.01.T01:intron 25.0%
!! TATTTAGGACATGATTGAAA+TGG + Chr4:69361173-69361192 MsG0480022181.01.T01:intron 25.0%
!! TTTTGTGTCTTTTGTATTGA+AGG - Chr4:69359804-69359823 None:intergenic 25.0%
!!! TGTTTCTTGTTTTTCAATAC+CGG + Chr4:69360167-69360186 MsG0480022181.01.T01:intron 25.0%
!!! TTTTTCGAAACTTAGGTAAT+TGG + Chr4:69360485-69360504 MsG0480022181.01.T01:intron 25.0%
AATAAACACACGGAAAAAAG+GGG - Chr4:69360005-69360024 None:intergenic 30.0%
AATTAATTGGGGAAAGAACA+AGG - Chr4:69360447-69360466 None:intergenic 30.0%
ACAAGCAGATAAAAAAGCAA+TGG - Chr4:69360539-69360558 None:intergenic 30.0%
AGGAAAAGTGAATAAACACA+CGG - Chr4:69360015-69360034 None:intergenic 30.0%
ATACATCATTCTCATATCAC+TGG + Chr4:69361101-69361120 MsG0480022181.01.T01:intron 30.0%
ATCTACCAAAATTCCACATT+AGG - Chr4:69361227-69361246 None:intergenic 30.0%
ATTGAAATGGACACTTTAGA+GGG + Chr4:69361186-69361205 MsG0480022181.01.T01:CDS 30.0%
CAATGTAAGTTATTGAATCC+CGG - Chr4:69360189-69360208 None:intergenic 30.0%
CTTTACAGCTTCATCTAATT+AGG + Chr4:69360272-69360291 MsG0480022181.01.T01:intron 30.0%
GAAACTTAGGTAATTGGTAT+TGG + Chr4:69360491-69360510 MsG0480022181.01.T01:intron 30.0%
GAATAAACACACGGAAAAAA+GGG - Chr4:69360006-69360025 None:intergenic 30.0%
GAATGATGTATCAAGAATCA+TGG - Chr4:69361092-69361111 None:intergenic 30.0%
TAATTGAAAAATCGTTCACG+AGG - Chr4:69360035-69360054 None:intergenic 30.0%
TATAATTCCTAACTTCAGGA+AGG + Chr4:69360394-69360413 MsG0480022181.01.T01:CDS 30.0%
TATAGTGTAACAGTATAGGA+TGG + Chr4:69360596-69360615 MsG0480022181.01.T01:intron 30.0%
TCGTAGCAAAATCAAAACAA+TGG + Chr4:69359846-69359865 MsG0480022181.01.T01:exon 30.0%
TGAATAAACACACGGAAAAA+AGG - Chr4:69360007-69360026 None:intergenic 30.0%
TGCTTATAGTGTAACAGTAT+AGG + Chr4:69360592-69360611 MsG0480022181.01.T01:intron 30.0%
TGTATGTTGGAATCAAATCA+CGG + Chr4:69360657-69360676 MsG0480022181.01.T01:intron 30.0%
TTGGGAATACTCTATGTATT+TGG + Chr4:69360510-69360529 MsG0480022181.01.T01:intron 30.0%
! CTGATATAGCCTTGAATTTA+AGG - Chr4:69360879-69360898 None:intergenic 30.0%
!!! ACATTCAAGTCACTTTTAAC+CGG - Chr4:69360812-69360831 None:intergenic 30.0%
!!! GTGTTTCTTTTTCGAAACTT+AGG + Chr4:69360478-69360497 MsG0480022181.01.T01:intron 30.0%
!!! GTTTCTTGTTTTTCAATACC+GGG + Chr4:69360168-69360187 MsG0480022181.01.T01:intron 30.0%
AAAAGGTGGAGTCAACAATA+TGG - Chr4:69361137-69361156 None:intergenic 35.0%
ACAAATATCAATTGCATCGC+CGG - Chr4:69360780-69360799 None:intergenic 35.0%
ACATATACCTTCCTGAAGTT+AGG - Chr4:69360404-69360423 None:intergenic 35.0%
ATAAACACACGGAAAAAAGG+GGG - Chr4:69360004-69360023 None:intergenic 35.0%
CGGGATTCAATAACTTACAT+TGG + Chr4:69360187-69360206 MsG0480022181.01.T01:intron 35.0%
GATTGAAATGGACACTTTAG+AGG + Chr4:69361185-69361204 MsG0480022181.01.T01:CDS 35.0%
GCGTTCATCATGAATAAAAG+AGG - Chr4:69360097-69360116 None:intergenic 35.0%
TGGATTGCACCTTAAATTCA+AGG + Chr4:69360867-69360886 MsG0480022181.01.T01:intron 35.0%
TTAGAGGGAAAATTGGAGTT+GGG + Chr4:69361201-69361220 MsG0480022181.01.T01:CDS 35.0%
TTTAGAGGGAAAATTGGAGT+TGG + Chr4:69361200-69361219 MsG0480022181.01.T01:CDS 35.0%
!!! ATGTTTTTCAGGGAACTACA+AGG + Chr4:69360360-69360379 MsG0480022181.01.T01:intron 35.0%
ATTCTCATATCACTGGATCC+AGG + Chr4:69361108-69361127 MsG0480022181.01.T01:intron 40.0%
GAGTCAACAATATGGAATCC+TGG - Chr4:69361129-69361148 None:intergenic 40.0%
GCGTATCACAGAACAAATAG+CGG + Chr4:69360925-69360944 MsG0480022181.01.T01:intron 40.0%
TAAACACACGGAAAAAAGGG+GGG - Chr4:69360003-69360022 None:intergenic 40.0%
TAATTGGGGAAAGAACAAGG+AGG - Chr4:69360444-69360463 None:intergenic 40.0%
TACTCCAACCTCAAAGTCTA+TGG - Chr4:69361019-69361038 None:intergenic 40.0%
TGATTCCAACATACACGAAG+TGG - Chr4:69360652-69360671 None:intergenic 40.0%
! GGACACTTTAGAGGGAAAAT+TGG + Chr4:69361194-69361213 MsG0480022181.01.T01:CDS 40.0%
! GTGTAGCTTTTGCAGAATCA+CGG + Chr4:69360709-69360728 MsG0480022181.01.T01:intron 40.0%
! TCTCTCTCTCTCGTATTTTC+TGG + Chr4:69359754-69359773 MsG0480022181.01.T01:five_prime_UTR 40.0%
! TTGGGCCTAATGTGGAATTT+TGG + Chr4:69361219-69361238 MsG0480022181.01.T01:CDS 40.0%
! TTTTGGTAGATTCTCACTCC+AGG + Chr4:69361236-69361255 MsG0480022181.01.T01:CDS 40.0%
AATTGGAGTTGGGCCTAATG+TGG + Chr4:69361211-69361230 MsG0480022181.01.T01:CDS 45.0%
AGAGAGCAAATCGTTGACGT+TGG + Chr4:69359877-69359896 MsG0480022181.01.T01:CDS 45.0%
AGCCGCTATTCAACAACACT+GGG + Chr4:69360991-69361010 MsG0480022181.01.T01:intron 45.0%
ATTCTCACTCCAGGTGGTTT+GGG + Chr4:69361245-69361264 MsG0480022181.01.T01:CDS 45.0%
GCAAATCGTTGACGTTGGAT+CGG + Chr4:69359882-69359901 MsG0480022181.01.T01:CDS 45.0%
TAGCCGCTATTCAACAACAC+TGG + Chr4:69360990-69361009 MsG0480022181.01.T01:intron 45.0%
! CACCCAGTGTTGTTGAATAG+CGG - Chr4:69360996-69361015 None:intergenic 45.0%
! GAGAGACTCGAGAGTGTAAA+AGG - Chr4:69359946-69359965 None:intergenic 45.0%
!!! ACCACGTGCGTTTTGTTTTC+CGG + Chr4:69360758-69360777 MsG0480022181.01.T01:intron 45.0%
ACTCTTTCTGCTGACGCTTC+TGG + Chr4:69359913-69359932 MsG0480022181.01.T01:CDS 50.0%
CTGCACCACTTCGTGTATGT+TGG + Chr4:69360644-69360663 MsG0480022181.01.T01:intron 50.0%
GATTCTCACTCCAGGTGGTT+TGG + Chr4:69361244-69361263 MsG0480022181.01.T01:CDS 50.0%
TGGTAGATTCTCACTCCAGG+TGG + Chr4:69361239-69361258 MsG0480022181.01.T01:CDS 50.0%
! ACTGGGTGCCATAGACTTTG+AGG + Chr4:69361008-69361027 MsG0480022181.01.T01:intron 50.0%
!! GGTGCCATAGACTTTGAGGT+TGG + Chr4:69361012-69361031 MsG0480022181.01.T01:intron 50.0%
CCATAAAGGCCCAAACCACC+TGG - Chr4:69361257-69361276 None:intergenic 55.0%
GCCGGAAAACAAAACGCACG+TGG - Chr4:69360762-69360781 None:intergenic 55.0%
! CCAGGTGGTTTGGGCCTTTA+TGG + Chr4:69361254-69361273 MsG0480022181.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 69359682 69361323 69359682 ID=MsG0480022181.01;Name=MsG0480022181.01
Chr4 mRNA 69359682 69361323 69359682 ID=MsG0480022181.01.T01;Parent=MsG0480022181.01;Name=MsG0480022181.01.T01;_AED=0.50;_eAED=0.66;_QI=161|0.25|0.6|1|0|0.2|5|0|108
Chr4 exon 69359682 69359699 69359682 ID=MsG0480022181.01.T01:exon:8280;Parent=MsG0480022181.01.T01
Chr4 exon 69359722 69359986 69359722 ID=MsG0480022181.01.T01:exon:8281;Parent=MsG0480022181.01.T01
Chr4 exon 69360250 69360265 69360250 ID=MsG0480022181.01.T01:exon:8282;Parent=MsG0480022181.01.T01
Chr4 exon 69360371 69360415 69360371 ID=MsG0480022181.01.T01:exon:8283;Parent=MsG0480022181.01.T01
Chr4 exon 69361180 69361323 69361180 ID=MsG0480022181.01.T01:exon:8284;Parent=MsG0480022181.01.T01
Chr4 five_prime_UTR 69359682 69359699 69359682 ID=MsG0480022181.01.T01:five_prime_utr;Parent=MsG0480022181.01.T01
Chr4 five_prime_UTR 69359722 69359864 69359722 ID=MsG0480022181.01.T01:five_prime_utr;Parent=MsG0480022181.01.T01
Chr4 CDS 69359865 69359986 69359865 ID=MsG0480022181.01.T01:cds;Parent=MsG0480022181.01.T01
Chr4 CDS 69360250 69360265 69360250 ID=MsG0480022181.01.T01:cds;Parent=MsG0480022181.01.T01
Chr4 CDS 69360371 69360415 69360371 ID=MsG0480022181.01.T01:cds;Parent=MsG0480022181.01.T01
Chr4 CDS 69361180 69361323 69361180 ID=MsG0480022181.01.T01:cds;Parent=MsG0480022181.01.T01
Gene Sequence

>MsG0480022181.01.T01

ATGGAAGCAAAAAGAGAGCAAATCGTTGACGTTGGATCGGTTGTCGAAACTCTTTCTGCTGACGCTTCTGGTGATGCACCTTTTACACTCTCGAGTCTCTCTGCATGCGCTGTCATCAAAATTTATTGTATTTTTAGGGGAACTACAAGGTTTTTATTTACTATAATTCCTAACTTCAGGAAGGACATGATTGAAATGGACACTTTAGAGGGAAAATTGGAGTTGGGCCTAATGTGGAATTTTGGTAGATTCTCACTCCAGGTGGTTTGGGCCTTTATGGAAAAAAATTGTAAAAGACCCAATAAAAAGGAAAAACAAAATGGATAG

Protein sequence

>MsG0480022181.01.T01

MEAKREQIVDVGSVVETLSADASGDAPFTLSSLSACAVIKIYCIFRGTTRFLFTIIPNFRKDMIEMDTLEGKLELGLMWNFGRFSLQVVWAFMEKNCKRPNKKEKQNG*