Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0402780 | XP_003601303.1 | 95.146 | 206 | 10 | 0 | 1 | 206 | 1 | 206 | 5.37e-125 | 361 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0402780 | sp|Q9LZH5|LTPG2_ARATH | 46.324 | 136 | 63 | 3 | 43 | 169 | 32 | 166 | 7.94e-27 | 104 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0402780 | B7FFE5 | 95.146 | 206 | 10 | 0 | 1 | 206 | 1 | 206 | 2.56e-125 | 361 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0027020 | Msa0402780 | 0.801623 | 8.251331e-49 | -8.615850e-47 |
| Msa0047630 | Msa0402780 | 0.814928 | 1.199867e-51 | -8.615850e-47 |
| Msa0051480 | Msa0402780 | -0.816026 | 6.836160e-52 | -8.615850e-47 |
| Msa0075820 | Msa0402780 | 0.803602 | 3.224158e-49 | -8.615850e-47 |
| Msa0078110 | Msa0402780 | 0.817488 | 3.212643e-52 | -8.615850e-47 |
| Msa0099300 | Msa0402780 | 0.809593 | 1.752768e-50 | -8.615850e-47 |
| Msa0104860 | Msa0402780 | -0.806154 | 9.438902e-50 | -8.615850e-47 |
| Msa0129770 | Msa0402780 | -0.841332 | 5.067512e-58 | -8.615850e-47 |
| Msa0129820 | Msa0402780 | 0.807587 | 4.700327e-50 | -8.615850e-47 |
| Msa0145590 | Msa0402780 | 0.806696 | 7.256494e-50 | -8.615850e-47 |
| Msa0146200 | Msa0402780 | -0.810634 | 1.045397e-50 | -8.615850e-47 |
| Msa0167160 | Msa0402780 | 0.809801 | 1.581448e-50 | -8.615850e-47 |
| Msa0184070 | Msa0402780 | 0.804781 | 1.832069e-49 | -8.615850e-47 |
| Msa0198280 | Msa0402780 | 0.807011 | 6.225416e-50 | -8.615850e-47 |
| Msa0232160 | Msa0402780 | 0.812767 | 3.592910e-51 | -8.615850e-47 |
| Msa0237690 | Msa0402780 | 0.803789 | 2.947598e-49 | -8.615850e-47 |
| Msa0369770 | Msa0402780 | 0.807575 | 4.727030e-50 | -8.615850e-47 |
| Msa0402780 | Msa0404840 | 0.817625 | 2.992372e-52 | -8.615850e-47 |
| Msa0402780 | Msa0417140 | 0.813610 | 2.345537e-51 | -8.615850e-47 |
| Msa0402780 | Msa0438070 | -0.816103 | 6.571345e-52 | -8.615850e-47 |
| Msa0402780 | Msa0444770 | 0.818034 | 2.419904e-52 | -8.615850e-47 |
| Msa0402780 | Msa0450830 | 0.816174 | 6.335422e-52 | -8.615850e-47 |
| Msa0402780 | Msa0463980 | 0.807944 | 3.946088e-50 | -8.615850e-47 |
| Msa0402780 | Msa0490440 | 0.920659 | 9.665901e-88 | -8.615850e-47 |
| Msa0402780 | Msa0498090 | 0.803325 | 3.679547e-49 | -8.615850e-47 |
| Msa0402780 | Msa0509530 | 0.808942 | 2.417319e-50 | -8.615850e-47 |
| Msa0402780 | Msa0514280 | 0.806050 | 9.928161e-50 | -8.615850e-47 |
| Msa0402780 | Msa0522820 | 0.815409 | 9.383132e-52 | -8.615850e-47 |
| Msa0402780 | Msa0523980 | -0.805319 | 1.413479e-49 | -8.615850e-47 |
| Msa0402780 | Msa0552920 | 0.802749 | 4.839928e-49 | -8.615850e-47 |
| Msa0402780 | Msa0608250 | 0.809098 | 2.238196e-50 | -8.615850e-47 |
| Msa0402780 | Msa0608280 | 0.811651 | 6.292544e-51 | -8.615850e-47 |
| Msa0402780 | Msa0615460 | 0.814542 | 1.460801e-51 | -8.615850e-47 |
| Msa0402780 | Msa0619870 | 0.807476 | 4.962423e-50 | -8.615850e-47 |
| Msa0402780 | Msa0643590 | 0.802029 | 6.809500e-49 | -8.615850e-47 |
| Msa0402780 | Msa0648840 | 0.816337 | 5.825076e-52 | -8.615850e-47 |
| Msa0402780 | Msa0654850 | 0.809430 | 1.899370e-50 | -8.615850e-47 |
| Msa0402780 | Msa0691350 | 0.802885 | 4.537463e-49 | -8.615850e-47 |
| Msa0402780 | Msa0695250 | 0.812432 | 4.253193e-51 | -8.615850e-47 |
| Msa0402780 | Msa0698510 | 0.802355 | 5.836250e-49 | -8.615850e-47 |
| Msa0402780 | Msa0698680 | 0.806345 | 8.607195e-50 | -8.615850e-47 |
| Msa0402780 | Msa0746640 | 0.917590 | 4.412978e-86 | -8.615850e-47 |
| Msa0402780 | Msa0751490 | 0.805603 | 1.232587e-49 | -8.615850e-47 |
| Msa0402780 | Msa0788450 | 0.809035 | 2.308788e-50 | -8.615850e-47 |
| Msa0402780 | Msa0816390 | 0.809717 | 1.647894e-50 | -8.615850e-47 |
| Msa0402780 | Msa0820130 | 0.810653 | 1.035977e-50 | -8.615850e-47 |
| Msa0402780 | Msa0825570 | 0.808311 | 3.296947e-50 | -8.615850e-47 |
| Msa0402780 | Msa0826540 | 0.811871 | 5.635865e-51 | -8.615850e-47 |
| Msa0402780 | Msa0860880 | 0.800096 | 1.691642e-48 | -8.615850e-47 |
| Msa0402780 | Msa0871850 | 0.805822 | 1.108586e-49 | -8.615850e-47 |
| Msa0402780 | Msa0871860 | 0.804646 | 1.954957e-49 | -8.615850e-47 |
| Msa0402780 | Msa0882480 | 0.806866 | 6.680123e-50 | -8.615850e-47 |
| Msa0402780 | Msa0888520 | 0.804665 | 1.937487e-49 | -8.615850e-47 |
| Msa0402780 | Msa0905210 | 0.802797 | 4.730192e-49 | -8.615850e-47 |
| Msa0402780 | Msa0937180 | 0.825239 | 5.224942e-54 | -8.615850e-47 |
| Msa0402780 | Msa0937190 | 0.811921 | 5.497432e-51 | -8.615850e-47 |
| Msa0402780 | Msa0945160 | 0.808632 | 2.815626e-50 | -8.615850e-47 |
| Msa0402780 | Msa0958960 | 0.803448 | 3.469568e-49 | -8.615850e-47 |
| Msa0402780 | Msa0966580 | -0.830901 | 2.261455e-55 | -8.615850e-47 |
| Msa0402780 | Msa0974480 | 0.800326 | 1.518755e-48 | -8.615850e-47 |
| Msa0402780 | Msa0982720 | 0.948225 | 1.438480e-106 | -8.615850e-47 |
| Msa0402780 | Msa1129920 | 0.806889 | 6.604854e-50 | -8.615850e-47 |
| Msa0402780 | Msa1130150 | 0.801238 | 9.896430e-49 | -8.615850e-47 |
| Msa0402780 | Msa1163130 | 0.810656 | 1.034316e-50 | -8.615850e-47 |
| Msa0402780 | Msa1197740 | 0.810118 | 1.351221e-50 | -8.615850e-47 |
| Msa0402780 | Msa1211930 | 0.832310 | 1.017102e-55 | -8.615850e-47 |
| Msa0402780 | Msa1216820 | 0.800348 | 1.503077e-48 | -8.615850e-47 |
| Msa0402780 | Msa1235620 | 0.827802 | 1.279265e-54 | -8.615850e-47 |
| Msa0402780 | Msa1251440 | 0.803158 | 3.983465e-49 | -8.615850e-47 |
| Msa0402780 | Msa1292060 | 0.801730 | 7.844428e-49 | -8.615850e-47 |
| Msa0402780 | Msa1334690 | 0.841704 | 4.043608e-58 | -8.615850e-47 |
| Msa0402780 | Msa1341850 | 0.814958 | 1.181841e-51 | -8.615850e-47 |
| Msa0402780 | Msa1347400 | 0.944304 | 2.491592e-103 | -8.615850e-47 |
| Msa0402780 | Msa1372460 | 0.813244 | 2.823561e-51 | -8.615850e-47 |
| Msa0402780 | Msa1377050 | -0.806638 | 7.463804e-50 | -8.615850e-47 |
| Msa0402780 | Msa1386820 | 0.837802 | 4.192027e-57 | -8.615850e-47 |
| Msa0402780 | Msa1395250 | -0.819624 | 1.053022e-52 | -8.615850e-47 |
| Msa0402780 | Msa1415510 | 0.806881 | 6.631401e-50 | -8.615850e-47 |
| Msa0402780 | Msa1434860 | 0.817312 | 3.520175e-52 | -8.615850e-47 |
| Msa0402780 | Msa1455560 | 0.811882 | 5.605326e-51 | -8.615850e-47 |
| Msa0247340 | Msa0402780 | 0.811103 | 8.275003e-51 | -8.615850e-47 |
| Msa0296100 | Msa0402780 | 0.811362 | 7.270455e-51 | -8.615850e-47 |
| Msa0298820 | Msa0402780 | 0.807149 | 5.818939e-50 | -8.615850e-47 |
| Msa0310270 | Msa0402780 | 0.815850 | 7.484466e-52 | -8.615850e-47 |
| Msa0334480 | Msa0402780 | 0.828572 | 8.345515e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0402780 | MtrunA17_Chr3g0118471 | 95.146 | 206 | 10 | 0 | 1 | 206 | 1 | 206 | 4.94e-129 | 361 |
| Msa0402780 | MtrunA17_Chr5g0395001 | 39.200 | 125 | 73 | 2 | 46 | 170 | 24 | 145 | 2.18e-18 | 78.6 |
| Msa0402780 | MtrunA17_Chr1g0205201 | 40.659 | 91 | 51 | 2 | 46 | 136 | 24 | 111 | 5.25e-16 | 72.0 |
| Msa0402780 | MtrunA17_Chr2g0278981 | 40.206 | 97 | 55 | 2 | 34 | 130 | 20 | 113 | 9.32e-16 | 72.4 |
| Msa0402780 | MtrunA17_Chr5g0429901 | 34.328 | 134 | 78 | 3 | 42 | 175 | 28 | 151 | 2.14e-15 | 71.2 |
| Msa0402780 | MtrunA17_Chr2g0278971 | 37.190 | 121 | 63 | 4 | 57 | 177 | 83 | 190 | 1.78e-14 | 69.3 |
| Msa0402780 | MtrunA17_Chr7g0260061 | 36.000 | 125 | 73 | 5 | 47 | 169 | 28 | 147 | 6.34e-13 | 64.7 |
| Msa0402780 | MtrunA17_Chr1g0205181 | 36.029 | 136 | 61 | 4 | 46 | 181 | 24 | 133 | 1.67e-12 | 62.8 |
| Msa0402780 | MtrunA17_Chr1g0205191 | 36.735 | 98 | 57 | 3 | 42 | 139 | 20 | 112 | 1.92e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0402780 | AT3G43720.2 | 47.015 | 134 | 63 | 3 | 43 | 169 | 32 | 164 | 1.36e-28 | 106 |
| Msa0402780 | AT3G43720.1 | 46.324 | 136 | 63 | 3 | 43 | 169 | 32 | 166 | 8.08e-28 | 104 |
| Msa0402780 | AT2G27130.1 | 43.519 | 108 | 59 | 2 | 34 | 140 | 24 | 130 | 1.03e-23 | 93.2 |
| Msa0402780 | AT1G03103.1 | 35.443 | 158 | 85 | 5 | 49 | 204 | 24 | 166 | 4.33e-16 | 73.2 |
| Msa0402780 | AT3G22600.1 | 40.698 | 86 | 48 | 2 | 46 | 131 | 24 | 106 | 8.18e-16 | 72.4 |
| Msa0402780 | AT2G48130.2 | 33.645 | 107 | 68 | 2 | 50 | 156 | 30 | 133 | 2.22e-15 | 71.6 |
| Msa0402780 | AT2G48130.6 | 33.645 | 107 | 68 | 2 | 50 | 156 | 30 | 133 | 2.22e-15 | 71.6 |
| Msa0402780 | AT2G48130.4 | 33.645 | 107 | 68 | 2 | 50 | 156 | 30 | 133 | 2.22e-15 | 71.6 |
| Msa0402780 | AT2G48130.5 | 33.645 | 107 | 68 | 2 | 50 | 156 | 30 | 133 | 2.22e-15 | 71.6 |
| Msa0402780 | AT2G48130.1 | 33.645 | 107 | 68 | 2 | 50 | 156 | 30 | 133 | 2.22e-15 | 71.6 |
| Msa0402780 | AT2G48130.3 | 33.645 | 107 | 68 | 2 | 50 | 156 | 30 | 133 | 2.22e-15 | 71.6 |
| Msa0402780 | AT4G08670.1 | 34.356 | 163 | 94 | 6 | 42 | 197 | 40 | 196 | 6.66e-15 | 70.9 |
| Msa0402780 | AT2G13820.1 | 35.772 | 123 | 75 | 2 | 50 | 170 | 27 | 147 | 1.10e-14 | 69.3 |
| Msa0402780 | AT2G13820.3 | 35.772 | 123 | 75 | 2 | 50 | 170 | 39 | 159 | 1.30e-14 | 69.3 |
| Msa0402780 | AT5G64080.1 | 30.909 | 165 | 93 | 5 | 42 | 206 | 36 | 179 | 8.12e-14 | 67.4 |
| Msa0402780 | AT5G64080.2 | 33.077 | 130 | 77 | 4 | 42 | 165 | 36 | 161 | 8.90e-14 | 67.0 |
| Msa0402780 | AT4G14815.1 | 35.484 | 93 | 57 | 2 | 40 | 132 | 16 | 105 | 3.01e-13 | 65.1 |
| Msa0402780 | AT5G09370.3 | 34.188 | 117 | 69 | 3 | 50 | 163 | 28 | 139 | 3.66e-13 | 65.1 |
| Msa0402780 | AT2G13820.2 | 37.662 | 77 | 46 | 1 | 54 | 130 | 31 | 105 | 8.11e-13 | 63.2 |
| Msa0402780 | AT5G09370.1 | 35.714 | 98 | 58 | 2 | 50 | 147 | 28 | 120 | 2.65e-12 | 62.8 |
| Msa0402780 | AT5G09370.2 | 37.931 | 87 | 49 | 2 | 60 | 146 | 38 | 119 | 1.49e-11 | 60.1 |
Find 68 sgRNAs with CRISPR-Local
Find 148 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTATTACACACTTTAATT+AGG | 0.225161 | 3_2:+31415609 | Msa0402780:three_prime_UTR |
| AGCCCTTCTGGAAGCAAAAT+TGG | 0.248172 | 3_2:+31415414 | Msa0402780:CDS |
| GCAGGTGTTCTTAGCCCTTC+TGG | 0.259291 | 3_2:+31415402 | Msa0402780:intron |
| AACAGCTTACACAAACAAAT+AGG | 0.265322 | 3_2:-31413279 | None:intergenic |
| GGCGCTAAAACCGCGGTTTC+CGG | 0.269928 | 3_2:-31413120 | None:intergenic |
| TATTGAGTGCTTTGCTTTAG+TGG | 0.271967 | 3_2:+31415582 | Msa0402780:three_prime_UTR |
| AACCTATCACAAAACTAAAA+TGG | 0.273800 | 3_2:+31412996 | Msa0402780:exon |
| TTTGCATCACTCTCATTTCA+TGG | 0.285544 | 3_2:+31413057 | Msa0402780:CDS |
| ATAAATAGAGGGACTTGATT+TGG | 0.291910 | 3_2:-31412857 | None:intergenic |
| ACTACCGAGTTCTTCGTTCT+TGG | 0.317095 | 3_2:+31415646 | Msa0402780:three_prime_UTR |
| AAAGCACTCAATACACAAAT+AGG | 0.323137 | 3_2:-31415572 | None:intergenic |
| TATATATAATATAGTCTATT+CGG | 0.351497 | 3_2:-31415792 | None:intergenic |
| TGGAGTATCTGCTGCTGCTT+TGG | 0.352720 | 3_2:+31413083 | Msa0402780:CDS |
| CCTCCTGTTAGTGCTTGTTC+AGG | 0.362697 | 3_2:+31413387 | Msa0402780:CDS |
| CTTAAGCTTCCAACTATTTG+TGG | 0.365023 | 3_2:+31413354 | Msa0402780:CDS |
| GGACTTAGTGCTGATCCTTT+TGG | 0.370348 | 3_2:-31415065 | None:intergenic |
| ATTTGTTTGTGTAAGCTGTT+AGG | 0.384262 | 3_2:+31413282 | Msa0402780:CDS |
| CGGTGCAAAATGAGTTAGTT+AGG | 0.388672 | 3_2:+31412779 | Msa0402780:five_prime_UTR |
| TGAATATGTGTTATTATGAT+TGG | 0.393179 | 3_2:-31412901 | None:intergenic |
| TCAACTTCATCTGATAGTTC+AGG | 0.394833 | 3_2:+31415089 | Msa0402780:CDS |
| GTCAGATTGTTTGACATTTG+TGG | 0.406541 | 3_2:+31413185 | Msa0402780:CDS |
| GAGTTAGCAGGGTTGATTGA+TGG | 0.411939 | 3_2:+31413252 | Msa0402780:CDS |
| AATGTAAAATCAGAAGAATA+TGG | 0.417381 | 3_2:-31415502 | None:intergenic |
| AGTGCTTTGCTTTAGTGGAT+TGG | 0.419671 | 3_2:+31415587 | Msa0402780:three_prime_UTR |
| TCAGGACAACATCCTTTGTC+TGG | 0.420386 | 3_2:-31413231 | None:intergenic |
| TGATTGGTATGTTATATAGT+TGG | 0.424649 | 3_2:-31412885 | None:intergenic |
| TTACCTGAACAAGCACTAAC+AGG | 0.424650 | 3_2:-31413390 | None:intergenic |
| TGTTTGACATTTGTGGAAGA+TGG | 0.446855 | 3_2:+31413192 | Msa0402780:CDS |
| CTATATAGCAAGAATGTAGT+TGG | 0.452894 | 3_2:-31415699 | None:intergenic |
| GGTAATGCTGCTGATTCTAT+TGG | 0.462047 | 3_2:+31413306 | Msa0402780:CDS |
| ACAGCTTACACAAACAAATA+GGG | 0.462564 | 3_2:-31413278 | None:intergenic |
| AAAACCGCGGTTTCCGGTGC+CGG | 0.471159 | 3_2:-31413114 | None:intergenic |
| GCGCCATCACCGGCAGATGA+TGG | 0.475243 | 3_2:+31413138 | Msa0402780:CDS |
| GGCAGATGATGGATGCTTGA+TGG | 0.475901 | 3_2:+31413149 | Msa0402780:CDS |
| GGATGTTGTCCTGAGTTAGC+AGG | 0.476788 | 3_2:+31413240 | Msa0402780:CDS |
| TGCTGCTTTGGCTCAATCAC+CGG | 0.486295 | 3_2:+31413095 | Msa0402780:CDS |
| GTTTGACATTTGTGGAAGAT+GGG | 0.488176 | 3_2:+31413193 | Msa0402780:CDS |
| TCACTCTCATTTCATGGAGA+TGG | 0.490910 | 3_2:+31413063 | Msa0402780:CDS |
| GTAGTAACACCACAAATAGT+TGG | 0.491016 | 3_2:-31413363 | None:intergenic |
| GAACTATCAGATGAAGTTGA+AGG | 0.493768 | 3_2:-31415086 | None:intergenic |
| CATCCATCATCTGCCGGTGA+TGG | 0.511093 | 3_2:-31413141 | None:intergenic |
| TAGTTGGAGTAAATAAATAG+AGG | 0.514530 | 3_2:-31412869 | None:intergenic |
| TTTGGCTCAATCACCGGCAC+CGG | 0.516118 | 3_2:+31413101 | Msa0402780:CDS |
| ACTCGGTAGTGTTGTGATGT+AGG | 0.516958 | 3_2:-31415633 | None:intergenic |
| TATATAGCAAGAATGTAGTT+GGG | 0.527922 | 3_2:-31415698 | None:intergenic |
| GATATGGCAATGTCACCAAA+AGG | 0.530694 | 3_2:+31415050 | Msa0402780:intron |
| CCTTCTGGAAGCAAAATTGG+AGG | 0.554339 | 3_2:+31415417 | Msa0402780:CDS |
| TGCAGAGGCTTGAATGCTTG+AGG | 0.572865 | 3_2:-31415442 | None:intergenic |
| TCAATCAACCCTGCTAACTC+AGG | 0.575923 | 3_2:-31413249 | None:intergenic |
| TCCACTCAGTGATGGAGGCA+AGG | 0.577085 | 3_2:-31414879 | None:intergenic |
| CGGTGATGGCGCTAAAACCG+CGG | 0.578610 | 3_2:-31413127 | None:intergenic |
| TGGAGACAGAGACAGTGGAC+AGG | 0.579721 | 3_2:-31415482 | None:intergenic |
| ATAGCATCTCCACTCAGTGA+TGG | 0.584097 | 3_2:-31414887 | None:intergenic |
| ATCAAGCATCCATCATCTGC+CGG | 0.595406 | 3_2:-31413147 | None:intergenic |
| GATGTTGTCCTGAGTTAGCA+GGG | 0.597137 | 3_2:+31413241 | Msa0402780:CDS |
| TGCAAAGAGTAAAGAAACAA+TGG | 0.599464 | 3_2:-31413040 | None:intergenic |
| AGTTGGAGTAAATAAATAGA+GGG | 0.601980 | 3_2:-31412868 | None:intergenic |
| GAATATGGAGACAGAGACAG+TGG | 0.605345 | 3_2:-31415487 | None:intergenic |
| GCATCTCCACTCAGTGATGG+AGG | 0.609350 | 3_2:-31414884 | None:intergenic |
| ATATGAAACTAATGACACAT+AGG | 0.618243 | 3_2:-31415534 | None:intergenic |
| GGAGACAGAGACAGTGGACA+GGG | 0.621999 | 3_2:-31415481 | None:intergenic |
| AGTGATGGAGGCAAGGACAC+AGG | 0.629179 | 3_2:-31414872 | None:intergenic |
| ATCACCAAGAACGAAGAACT+CGG | 0.632446 | 3_2:-31415650 | None:intergenic |
| TGTTTGTGTAAGCTGTTAGG+TGG | 0.635667 | 3_2:+31413285 | Msa0402780:CDS |
| TCCTTGCCTCCATCACTGAG+TGG | 0.643834 | 3_2:+31414878 | Msa0402780:CDS |
| ATCACCGGCACCGGAAACCG+CGG | 0.645420 | 3_2:+31413110 | Msa0402780:CDS |
| AAGTTGACAAAACCAGACAA+AGG | 0.698102 | 3_2:+31413219 | Msa0402780:CDS |
| CCTGAACAAGCACTAACAGG+AGG | 0.707608 | 3_2:-31413387 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGTTCAAATTTTAAAATATT+TGG | + | chr3_2:31413450-31413469 | Msa0402780:intron | 10.0% |
| !! | AAACATTACTTATATATATA+TGG | + | chr3_2:31413505-31413524 | Msa0402780:intron | 10.0% |
| !! | TATAATTAATAACTAAATTG+TGG | + | chr3_2:31413730-31413749 | Msa0402780:intron | 10.0% |
| !! | CAAACTTAAAAATAATAATT+TGG | + | chr3_2:31413960-31413979 | Msa0402780:intron | 10.0% |
| !! | TTAAAATAATGTTAAAACAA+AGG | - | chr3_2:31414114-31414133 | None:intergenic | 10.0% |
| !! | TATATATAATATAGTCTATT+CGG | - | chr3_2:31415795-31415814 | None:intergenic | 10.0% |
| !!! | ATCTTAAAATGTGATTTTTT+TGG | + | chr3_2:31413827-31413846 | Msa0402780:intron | 15.0% |
| !!! | TATTTTTAAGTTTGTTTGAT+AGG | - | chr3_2:31413954-31413973 | None:intergenic | 15.0% |
| !! | TTATATATTACCAATGATTA+TGG | + | chr3_2:31414002-31414021 | Msa0402780:intron | 15.0% |
| !! | GTTAAAATATACACTATAAT+TGG | + | chr3_2:31414164-31414183 | Msa0402780:intron | 15.0% |
| !! | ACAATTATTAAATTCAAAGA+AGG | - | chr3_2:31414288-31414307 | None:intergenic | 15.0% |
| !! | ATATGAATTATTCAAAAAGT+TGG | - | chr3_2:31414348-31414367 | None:intergenic | 15.0% |
| !!! | GTGTTACAATTATTTATTTT+GGG | - | chr3_2:31414625-31414644 | None:intergenic | 15.0% |
| !! | AAAAGAAAATAAAAAAAGAG+TGG | - | chr3_2:31414680-31414699 | None:intergenic | 15.0% |
| !! | AACAACAATAAATTAACTTA+TGG | - | chr3_2:31414930-31414949 | None:intergenic | 15.0% |
| !! | TTGATAAAATAATGAAGATA+TGG | + | chr3_2:31415034-31415053 | Msa0402780:intron | 15.0% |
| !! | TAATGATCATAAATATCAAT+CGG | + | chr3_2:31415202-31415221 | Msa0402780:intron | 15.0% |
| !! | TGGATCCAAAAATAAATAAA+CGG | + | chr3_2:31412759-31412778 | Msa0402780:five_prime_UTR | 20.0% |
| !!! | AAAAATGAGTTTCTAACTTT+TGG | - | chr3_2:31412837-31412856 | None:intergenic | 20.0% |
| !!! | TGAATATGTGTTATTATGAT+TGG | - | chr3_2:31412904-31412923 | None:intergenic | 20.0% |
| !! | GATTATTTGTCTTTCATATT+TGG | + | chr3_2:31413591-31413610 | Msa0402780:intron | 20.0% |
| !!! | CATCCTAAAGTTTTTTTTTT+AGG | + | chr3_2:31413756-31413775 | Msa0402780:intron | 20.0% |
| !! | GATCCTAAAAAAAAAACTTT+AGG | - | chr3_2:31413762-31413781 | None:intergenic | 20.0% |
| !! | ATTATTAAATTCAAAGAAGG+CGG | - | chr3_2:31414285-31414304 | None:intergenic | 20.0% |
| !! | TAATATTGCAATTACTAGTA+AGG | + | chr3_2:31414543-31414562 | Msa0402780:intron | 20.0% |
| !!! | TTTATTTGTTGAATGGTAAA+GGG | - | chr3_2:31414581-31414600 | None:intergenic | 20.0% |
| !!! | ATTTATTTGTTGAATGGTAA+AGG | - | chr3_2:31414582-31414601 | None:intergenic | 20.0% |
| !!! | TTGCAAATTTATTTGTTGAA+TGG | - | chr3_2:31414588-31414607 | None:intergenic | 20.0% |
| !!! | GGTGTTACAATTATTTATTT+TGG | - | chr3_2:31414626-31414645 | None:intergenic | 20.0% |
| !! | TATGTAAATTTACATGAACT+TGG | + | chr3_2:31414765-31414784 | Msa0402780:intron | 20.0% |
| !! | TAACTTAAGTAAAACTCATT+TGG | + | chr3_2:31415160-31415179 | Msa0402780:intron | 20.0% |
| !!! | GCAAGTTTTTTTTTTTTTGA+AGG | + | chr3_2:31415238-31415257 | Msa0402780:intron | 20.0% |
| !! | TTGATGGAAAATTCTATATT+TGG | - | chr3_2:31415337-31415356 | None:intergenic | 20.0% |
| !! | AATGTAAAATCAGAAGAATA+TGG | - | chr3_2:31415505-31415524 | None:intergenic | 20.0% |
| !! | GATTATTACACACTTTAATT+AGG | + | chr3_2:31415609-31415628 | Msa0402780:three_prime_UTR | 20.0% |
| !!! | TTGCACCGTTTATTTATTTT+TGG | - | chr3_2:31412767-31412786 | None:intergenic | 25.0% |
| ! | AGTTGGAGTAAATAAATAGA+GGG | - | chr3_2:31412871-31412890 | None:intergenic | 25.0% |
| ! | TAGTTGGAGTAAATAAATAG+AGG | - | chr3_2:31412872-31412891 | None:intergenic | 25.0% |
| ! | TGATTGGTATGTTATATAGT+TGG | - | chr3_2:31412888-31412907 | None:intergenic | 25.0% |
| !!! | TTTAGTTTTGTGATAGGTTT+TGG | - | chr3_2:31412995-31413014 | None:intergenic | 25.0% |
| ! | AACCTATCACAAAACTAAAA+TGG | + | chr3_2:31412996-31413015 | Msa0402780:exon | 25.0% |
| ! | TTTATGATTATGTGAGAAGT+TGG | + | chr3_2:31413704-31413723 | Msa0402780:intron | 25.0% |
| !!! | TATTTAGTTAGCCATTTTGT+CGG | + | chr3_2:31413796-31413815 | Msa0402780:intron | 25.0% |
| ! | CAATAATAGACCATAATCAT+TGG | - | chr3_2:31414015-31414034 | None:intergenic | 25.0% |
| ! | GAACAATTAATAACAGACAT+GGG | - | chr3_2:31414467-31414486 | None:intergenic | 25.0% |
| ! | TGAACAATTAATAACAGACA+TGG | - | chr3_2:31414468-31414487 | None:intergenic | 25.0% |
| ! | TAATTGCAATATTATTGCTG+TGG | - | chr3_2:31414537-31414556 | None:intergenic | 25.0% |
| ! | AAATTTGCAATAAGTGATGT+AGG | + | chr3_2:31414598-31414617 | Msa0402780:intron | 25.0% |
| ! | ACAAATTAAGGTGAACAATT+TGG | - | chr3_2:31414653-31414672 | None:intergenic | 25.0% |
| ! | TAACTTATGGAAAACTTACT+TGG | - | chr3_2:31414917-31414936 | None:intergenic | 25.0% |
| ! | CATGCACATAAAAGTTAATA+AGG | - | chr3_2:31415285-31415304 | None:intergenic | 25.0% |
| !! | CTTATTAACTTTTATGTGCA+TGG | + | chr3_2:31415283-31415302 | Msa0402780:intron | 25.0% |
| ! | ACTTTAGTACTATATGTTGA+TGG | - | chr3_2:31415353-31415372 | None:intergenic | 25.0% |
| ! | ATATGAAACTAATGACACAT+AGG | - | chr3_2:31415537-31415556 | None:intergenic | 25.0% |
| ! | TATATAGCAAGAATGTAGTT+GGG | - | chr3_2:31415701-31415720 | None:intergenic | 25.0% |
| !! | AATTTTGAGAGGATAGAAGT+TGG | + | chr3_2:31412739-31412758 | Msa0402780:five_prime_UTR | 30.0% |
| ! | AATGAGTTTCTAACTTTTGG+TGG | - | chr3_2:31412834-31412853 | None:intergenic | 30.0% |
| ATAAATAGAGGGACTTGATT+TGG | - | chr3_2:31412860-31412879 | None:intergenic | 30.0% | |
| !!! | AGCCATTTTAGTTTTGTGAT+AGG | - | chr3_2:31413001-31413020 | None:intergenic | 30.0% |
| TGCAAAGAGTAAAGAAACAA+TGG | - | chr3_2:31413043-31413062 | None:intergenic | 30.0% | |
| ACAGCTTACACAAACAAATA+GGG | - | chr3_2:31413281-31413300 | None:intergenic | 30.0% | |
| AACAGCTTACACAAACAAAT+AGG | - | chr3_2:31413282-31413301 | None:intergenic | 30.0% | |
| ATTTGTTTGTGTAAGCTGTT+AGG | + | chr3_2:31413282-31413301 | Msa0402780:CDS | 30.0% | |
| AAACAAAGGTAACACTATGT+TGG | - | chr3_2:31414100-31414119 | None:intergenic | 30.0% | |
| ATTGCAATTACTAGTAAGGT+AGG | + | chr3_2:31414547-31414566 | Msa0402780:intron | 30.0% | |
| TTGCAATTACTAGTAAGGTA+GGG | + | chr3_2:31414548-31414567 | Msa0402780:intron | 30.0% | |
| TATATATGTGTCACAGCTAT+TGG | + | chr3_2:31414845-31414864 | Msa0402780:intron | 30.0% | |
| !! | GTATTTTTCAAAATGTGTGC+AGG | + | chr3_2:31415384-31415403 | Msa0402780:intron | 30.0% |
| ! | AAAGCACTCAATACACAAAT+AGG | - | chr3_2:31415575-31415594 | None:intergenic | 30.0% |
| CTATATAGCAAGAATGTAGT+TGG | - | chr3_2:31415702-31415721 | None:intergenic | 30.0% | |
| !! | CTTTTGGTGGCAGAATTTAA+TGG | - | chr3_2:31412821-31412840 | None:intergenic | 35.0% |
| !!! | TTGTGATAGGTTTTGGACTT+TGG | - | chr3_2:31412988-31413007 | None:intergenic | 35.0% |
| TTTGCATCACTCTCATTTCA+TGG | + | chr3_2:31413057-31413076 | Msa0402780:CDS | 35.0% | |
| GTCAGATTGTTTGACATTTG+TGG | + | chr3_2:31413185-31413204 | Msa0402780:CDS | 35.0% | |
| TGTTTGACATTTGTGGAAGA+TGG | + | chr3_2:31413192-31413211 | Msa0402780:CDS | 35.0% | |
| GTTTGACATTTGTGGAAGAT+GGG | + | chr3_2:31413193-31413212 | Msa0402780:CDS | 35.0% | |
| AAGTTGACAAAACCAGACAA+AGG | + | chr3_2:31413219-31413238 | Msa0402780:CDS | 35.0% | |
| CTTAAGCTTCCAACTATTTG+TGG | + | chr3_2:31413354-31413373 | Msa0402780:CDS | 35.0% | |
| GTAGTAACACCACAAATAGT+TGG | - | chr3_2:31413366-31413385 | None:intergenic | 35.0% | |
| GATCAAAATAGCCGACAAAA+TGG | - | chr3_2:31413810-31413829 | None:intergenic | 35.0% | |
| CTTCTTAGGTTTAGGTATGT+TGG | - | chr3_2:31414064-31414083 | None:intergenic | 35.0% | |
| TTGTCTCTCTTCTTAGGTTT+AGG | - | chr3_2:31414072-31414091 | None:intergenic | 35.0% | |
| GTAACTTTGTCTCTCTTCTT+AGG | - | chr3_2:31414078-31414097 | None:intergenic | 35.0% | |
| AATTCCATTACACATGGTGA+TGG | - | chr3_2:31414229-31414248 | None:intergenic | 35.0% | |
| ! | ATCACCATGTGTAATGGAAT+TGG | + | chr3_2:31414228-31414247 | Msa0402780:intron | 35.0% |
| ! | TGTGGTTGGCTAACTAAATA+TGG | - | chr3_2:31414519-31414538 | None:intergenic | 35.0% |
| TGCAATATTATTGCTGTGGT+TGG | - | chr3_2:31414533-31414552 | None:intergenic | 35.0% | |
| ! | TAAGGTGAACAATTTGGAGT+TGG | - | chr3_2:31414647-31414666 | None:intergenic | 35.0% |
| AAGAGTGGCATGACAAATTA+AGG | - | chr3_2:31414665-31414684 | None:intergenic | 35.0% | |
| GAAATTACCTTGATCATCTG+TGG | + | chr3_2:31414993-31415012 | Msa0402780:intron | 35.0% | |
| TAAGTTACCACAGATGATCA+AGG | - | chr3_2:31415003-31415022 | None:intergenic | 35.0% | |
| GAACTATCAGATGAAGTTGA+AGG | - | chr3_2:31415089-31415108 | None:intergenic | 35.0% | |
| TCAACTTCATCTGATAGTTC+AGG | + | chr3_2:31415089-31415108 | Msa0402780:CDS | 35.0% | |
| AAAAATGAAAGTCAGTGCAG+AGG | - | chr3_2:31415460-31415479 | None:intergenic | 35.0% | |
| TATTGAGTGCTTTGCTTTAG+TGG | + | chr3_2:31415582-31415601 | Msa0402780:three_prime_UTR | 35.0% | |
| CGGTGCAAAATGAGTTAGTT+AGG | + | chr3_2:31412779-31412798 | Msa0402780:five_prime_UTR | 40.0% | |
| TCACTCTCATTTCATGGAGA+TGG | + | chr3_2:31413063-31413082 | Msa0402780:CDS | 40.0% | |
| TGTTTGTGTAAGCTGTTAGG+TGG | + | chr3_2:31413285-31413304 | Msa0402780:CDS | 40.0% | |
| !! | GGTAATGCTGCTGATTCTAT+TGG | + | chr3_2:31413306-31413325 | Msa0402780:CDS | 40.0% |
| TTACCTGAACAAGCACTAAC+AGG | - | chr3_2:31413393-31413412 | None:intergenic | 40.0% | |
| CTCATCATTGTCTCTCATCT+AGG | - | chr3_2:31413661-31413680 | None:intergenic | 40.0% | |
| AGAGACAATGATGAGTGAAG+AGG | + | chr3_2:31413666-31413685 | Msa0402780:intron | 40.0% | |
| GAGACAATGATGAGTGAAGA+GGG | + | chr3_2:31413667-31413686 | Msa0402780:intron | 40.0% | |
| CTAGCCAATTCCATTACACA+TGG | - | chr3_2:31414235-31414254 | None:intergenic | 40.0% | |
| ! | GCTAACTAAATATGGTGGAG+AGG | - | chr3_2:31414511-31414530 | None:intergenic | 40.0% |
| ! | GGTTGGCTAACTAAATATGG+TGG | - | chr3_2:31414516-31414535 | None:intergenic | 40.0% |
| GATATGGCAATGTCACCAAA+AGG | + | chr3_2:31415050-31415069 | Msa0402780:intron | 40.0% | |
| ! | CTCCAATTTTGCTTCCAGAA+GGG | - | chr3_2:31415419-31415438 | None:intergenic | 40.0% |
| ! | AGTGCTTTGCTTTAGTGGAT+TGG | + | chr3_2:31415587-31415606 | Msa0402780:three_prime_UTR | 40.0% |
| ATCACCAAGAACGAAGAACT+CGG | - | chr3_2:31415653-31415672 | None:intergenic | 40.0% | |
| ATCAAGCATCCATCATCTGC+CGG | - | chr3_2:31413150-31413169 | None:intergenic | 45.0% | |
| TCAGGACAACATCCTTTGTC+TGG | - | chr3_2:31413234-31413253 | None:intergenic | 45.0% | |
| GATGTTGTCCTGAGTTAGCA+GGG | + | chr3_2:31413241-31413260 | Msa0402780:CDS | 45.0% | |
| TCAATCAACCCTGCTAACTC+AGG | - | chr3_2:31413252-31413271 | None:intergenic | 45.0% | |
| ! | GAGTTAGCAGGGTTGATTGA+TGG | + | chr3_2:31413252-31413271 | Msa0402780:CDS | 45.0% |
| !! | ACATGGTGATGGACTGACAT+TGG | - | chr3_2:31414218-31414237 | None:intergenic | 45.0% |
| CAGTCCATCACCATGTGTAA+TGG | + | chr3_2:31414222-31414241 | Msa0402780:intron | 45.0% | |
| GAAGGCGGTATCAACAAAGA+AGG | - | chr3_2:31414270-31414289 | None:intergenic | 45.0% | |
| ATAGCATCTCCACTCAGTGA+TGG | - | chr3_2:31414890-31414909 | None:intergenic | 45.0% | |
| !!! | GGACTTAGTGCTGATCCTTT+TGG | - | chr3_2:31415068-31415087 | None:intergenic | 45.0% |
| ! | AGCCCTTCTGGAAGCAAAAT+TGG | + | chr3_2:31415414-31415433 | Msa0402780:CDS | 45.0% |
| ! | CCTCCAATTTTGCTTCCAGA+AGG | - | chr3_2:31415420-31415439 | None:intergenic | 45.0% |
| ! | CCTTCTGGAAGCAAAATTGG+AGG | + | chr3_2:31415417-31415436 | Msa0402780:CDS | 45.0% |
| GAATATGGAGACAGAGACAG+TGG | - | chr3_2:31415490-31415509 | None:intergenic | 45.0% | |
| ACTCGGTAGTGTTGTGATGT+AGG | - | chr3_2:31415636-31415655 | None:intergenic | 45.0% | |
| ACTACCGAGTTCTTCGTTCT+TGG | + | chr3_2:31415646-31415665 | Msa0402780:three_prime_UTR | 45.0% | |
| ! | TGGAGTATCTGCTGCTGCTT+TGG | + | chr3_2:31413083-31413102 | Msa0402780:CDS | 50.0% |
| !! | TGCTGCTTTGGCTCAATCAC+CGG | + | chr3_2:31413095-31413114 | Msa0402780:CDS | 50.0% |
| !! | GGCAGATGATGGATGCTTGA+TGG | + | chr3_2:31413149-31413168 | Msa0402780:CDS | 50.0% |
| GGATGTTGTCCTGAGTTAGC+AGG | + | chr3_2:31413240-31413259 | Msa0402780:CDS | 50.0% | |
| CCTGAACAAGCACTAACAGG+AGG | - | chr3_2:31413390-31413409 | None:intergenic | 50.0% | |
| CCTCCTGTTAGTGCTTGTTC+AGG | + | chr3_2:31413387-31413406 | Msa0402780:CDS | 50.0% | |
| TGCAGAGGCTTGAATGCTTG+AGG | - | chr3_2:31415445-31415464 | None:intergenic | 50.0% | |
| ! | TTTGGCTCAATCACCGGCAC+CGG | + | chr3_2:31413101-31413120 | Msa0402780:CDS | 55.0% |
| CATCCATCATCTGCCGGTGA+TGG | - | chr3_2:31413144-31413163 | None:intergenic | 55.0% | |
| ! | AGTGATGGAGGCAAGGACAC+AGG | - | chr3_2:31414875-31414894 | None:intergenic | 55.0% |
| TCCTTGCCTCCATCACTGAG+TGG | + | chr3_2:31414878-31414897 | Msa0402780:CDS | 55.0% | |
| ! | TCCACTCAGTGATGGAGGCA+AGG | - | chr3_2:31414882-31414901 | None:intergenic | 55.0% |
| GCATCTCCACTCAGTGATGG+AGG | - | chr3_2:31414887-31414906 | None:intergenic | 55.0% | |
| GCAGGTGTTCTTAGCCCTTC+TGG | + | chr3_2:31415402-31415421 | Msa0402780:intron | 55.0% | |
| GGAGACAGAGACAGTGGACA+GGG | - | chr3_2:31415484-31415503 | None:intergenic | 55.0% | |
| TGGAGACAGAGACAGTGGAC+AGG | - | chr3_2:31415485-31415504 | None:intergenic | 55.0% | |
| AAAACCGCGGTTTCCGGTGC+CGG | - | chr3_2:31413117-31413136 | None:intergenic | 60.0% | |
| GGCGCTAAAACCGCGGTTTC+CGG | - | chr3_2:31413123-31413142 | None:intergenic | 60.0% | |
| ! | CGGTGATGGCGCTAAAACCG+CGG | - | chr3_2:31413130-31413149 | None:intergenic | 60.0% |
| !! | CGCGGTTTTAGCGCCATCAC+CGG | + | chr3_2:31413128-31413147 | Msa0402780:CDS | 60.0% |
| ! | ATCACCGGCACCGGAAACCG+CGG | + | chr3_2:31413110-31413129 | Msa0402780:CDS | 65.0% |
| GCGCCATCACCGGCAGATGA+TGG | + | chr3_2:31413138-31413157 | Msa0402780:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 31412737 | 31415831 | 31412737 | ID=Msa0402780;Name=Msa0402780 |
| chr3_2 | mRNA | 31412737 | 31415831 | 31412737 | ID=Msa0402780-mRNA-1;Parent=Msa0402780;Name=Msa0402780-mRNA-1;_AED=0.00;_eAED=0.00;_QI=278|1|1|1|1|1|4|316|206 |
| chr3_2 | exon | 31412737 | 31413408 | 31412737 | ID=Msa0402780-mRNA-1:exon:6257;Parent=Msa0402780-mRNA-1 |
| chr3_2 | exon | 31414861 | 31414917 | 31414861 | ID=Msa0402780-mRNA-1:exon:6258;Parent=Msa0402780-mRNA-1 |
| chr3_2 | exon | 31415051 | 31415110 | 31415051 | ID=Msa0402780-mRNA-1:exon:6259;Parent=Msa0402780-mRNA-1 |
| chr3_2 | exon | 31415406 | 31415831 | 31415406 | ID=Msa0402780-mRNA-1:exon:6260;Parent=Msa0402780-mRNA-1 |
| chr3_2 | five_prime_UTR | 31412737 | 31413014 | 31412737 | ID=Msa0402780-mRNA-1:five_prime_utr;Parent=Msa0402780-mRNA-1 |
| chr3_2 | CDS | 31413015 | 31413408 | 31413015 | ID=Msa0402780-mRNA-1:cds;Parent=Msa0402780-mRNA-1 |
| chr3_2 | CDS | 31414861 | 31414917 | 31414861 | ID=Msa0402780-mRNA-1:cds;Parent=Msa0402780-mRNA-1 |
| chr3_2 | CDS | 31415051 | 31415110 | 31415051 | ID=Msa0402780-mRNA-1:cds;Parent=Msa0402780-mRNA-1 |
| chr3_2 | CDS | 31415406 | 31415515 | 31415406 | ID=Msa0402780-mRNA-1:cds;Parent=Msa0402780-mRNA-1 |
| chr3_2 | three_prime_UTR | 31415516 | 31415831 | 31415516 | ID=Msa0402780-mRNA-1:three_prime_utr;Parent=Msa0402780-mRNA-1 |
| Gene Sequence |
| Protein sequence |
