AlfalfaGEDB | Msa1292060

M. sativa cultivar zhongmu-4 / Msa1292060

KEGG: K15109
GO: GO:0000064 GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0006810 GO:0006811 GO:0006812 GO:0006820 GO:0006839 GO:0006855 GO:0006865 GO:0008150 GO:0008324 GO:0008509 GO:0008514 GO:0009507 GO:0009536 GO:0015075 GO:0015171 GO:0015179 GO:0015238 GO:0015318 GO:0015711 GO:0015807 GO:0015822 GO:0015849 GO:0015893 GO:0016020 GO:0016021 GO:0019866 GO:0022857 GO:0031090 GO:0031224 GO:0031966 GO:0031967 GO:0031975 GO:0034220 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046942 GO:0046943 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0098655 GO:0098656 GO:1902475 GO:1903352 GO:1903825 GO:1905039
PFAM: PF00153.30

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1292060	sp\|Q93XM7\|MCAT_ARATH	79.000	300	60	1	1	297	1	300	2.47e-173	484

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1292060	A0A396GRW5	97.643	297	7	0	1	297	2	298	0.0	582

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000220	Msa1292060	0.816815	4.552782e-52	-8.615850e-47
Msa0000610	Msa1292060	0.827053	1.934932e-54	-8.615850e-47
Msa0000800	Msa1292060	-0.811545	6.635220e-51	-8.615850e-47
Msa0001040	Msa1292060	0.801280	9.698720e-49	-8.615850e-47
Msa0001510	Msa1292060	0.922488	9.213529e-89	-8.615850e-47
Msa0001550	Msa1292060	0.822022	2.957756e-53	-8.615850e-47
Msa0002110	Msa1292060	0.903411	3.566968e-79	-8.615850e-47
Msa0002690	Msa1292060	0.935026	1.618278e-96	-8.615850e-47
Msa0002700	Msa1292060	0.935703	5.580295e-97	-8.615850e-47
Msa0003340	Msa1292060	0.843596	1.271714e-58	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1292060	MtrunA17_Chr8g0366791	97.643	297	7	0	1	297	2	298	0.0	582
Msa1292060	MtrunA17_Chr5g0411411	84.848	297	45	0	1	297	2	298	0.0	519
Msa1292060	MtrunA17_Chr4g0004571	35.640	289	168	6	6	291	14	287	8.92e-46	156
Msa1292060	MtrunA17_Chr4g0046981	34.194	310	182	9	1	297	1	301	2.95e-43	150
Msa1292060	MtrunA17_Chr2g0331771	35.667	300	174	6	1	296	149	433	1.57e-41	148
Msa1292060	MtrunA17_Chr2g0296061	29.467	319	184	9	9	296	16	324	7.24e-35	128
Msa1292060	MtrunA17_Chr5g0396801	31.761	318	181	7	4	297	34	339	1.36e-33	125
Msa1292060	MtrunA17_Chr5g0396801	28.922	204	129	4	100	296	30	224	3.97e-14	72.0
Msa1292060	MtrunA17_Chr8g0347451	31.056	322	184	8	2	297	45	354	2.09e-31	120
Msa1292060	MtrunA17_Chr4g0067231	27.554	323	191	8	7	296	13	325	3.63e-31	119

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1292060	AT5G46800.2	79.000	300	60	1	1	297	1	300	2.52e-174	484
Msa1292060	AT5G46800.3	79.000	300	60	1	1	297	1	300	2.52e-174	484
Msa1292060	AT5G46800.1	79.000	300	60	1	1	297	1	300	2.52e-174	484
Msa1292060	AT2G33820.1	33.987	306	174	8	6	297	16	307	6.50e-49	165
Msa1292060	AT2G33820.3	33.121	314	174	9	6	297	16	315	4.44e-46	158
Msa1292060	AT1G79900.1	33.808	281	153	7	22	296	30	283	3.85e-41	144
Msa1292060	AT2G33820.4	31.890	254	144	8	6	243	16	256	5.50e-33	122
Msa1292060	AT2G33820.2	35.938	192	106	5	115	297	43	226	2.94e-32	119
Msa1292060	AT5G48970.1	28.615	325	186	9	7	296	21	334	2.16e-31	120
Msa1292060	AT4G01100.1	30.218	321	186	8	3	297	37	345	5.77e-31	119

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Find 143 sgRNAs with CRISPR-Local

Find 297 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCCACTGAGGGATTCAA+AGG	0.580482	8_3:-47976441	Msa1292060:CDS
AAAGGCCTATACAAGGGTTT+TGG	0.323072	8_3:-47976423	Msa1292060:CDS
AAAGGCTTACTTCCCACAAT+GGG	0.478747	8_3:-47976702	Msa1292060:CDS
AAATGTAGTAAGAAATGGAT+TGG	0.594525	8_3:+47980116	None:intergenic
AAGAAATGGATTGGTGGGAT+TGG	0.464437	8_3:+47980125	None:intergenic
AAGAAGCATGCTGCATTTGC+AGG	0.353862	8_3:+47976378	None:intergenic
AAGGCTGCAACCGTAGCAAG+CGG	0.590188	8_3:+47977757	None:intergenic
AAGGGAAGATTGGGAATCAG+AGG	0.601082	8_3:+47980150	None:intergenic
AAGGGGTTTATACAAAGGTA+TGG	0.477080	8_3:-47977785	Msa1292060:CDS
AATAATAAAAGGATGTATGT+TGG	0.503449	8_3:+47976129	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GGTTGAAATGTAGTAAGAAA+TGG	+	chr8_3:47976150-47976169	None:intergenic	30.0%
GTTAAAAGAATCTGAGAGAT+GGG	+	chr8_3:47976289-47976308	None:intergenic	30.0%
AGAATGAATCGATCAATGAA+AGG	+	chr8_3:47976345-47976364	None:intergenic	30.0%
AGTTATTCCTAATAACTTGG+AGG	+	chr8_3:47976409-47976428	None:intergenic	30.0%
CTGATATGATAGAATATAGC+AGG	+	chr8_3:47976549-47976568	None:intergenic	30.0%
ATCATATCAGTATCATTGCT+CGG	-	chr8_3:47976559-47976578	Msa1292060:CDS	30.0%
AATTACTACGAGTTTCTATG+TGG	+	chr8_3:47976663-47976682	None:intergenic	30.0%
CCTCTATAACCTATTTACAA+AGG	+	chr8_3:47977276-47977295	None:intergenic	30.0%
CCTTTGTAAATAGGTTATAG+AGG	-	chr8_3:47977273-47977292	Msa1292060:intron	30.0%
ATACAATTGCTCAAGAAAAG+CGG	+	chr8_3:47977332-47977351	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	47976019	47980261	47976019	ID=Msa1292060;Name=Msa1292060
chr8_3	mRNA	47976019	47980261	47976019	ID=Msa1292060-mRNA-1;Parent=Msa1292060;Name=Msa1292060-mRNA-1;_AED=0.01;_eAED=0.01;_QI=370\|1\|1\|1\|0.5\|0.66\|3\|325\|297
chr8_3	exon	47976019	47976845	47976019	ID=Msa1292060-mRNA-1:exon:13302;Parent=Msa1292060-mRNA-1
chr8_3	exon	47977601	47977992	47977601	ID=Msa1292060-mRNA-1:exon:13301;Parent=Msa1292060-mRNA-1
chr8_3	exon	47979892	47980261	47979892	ID=Msa1292060-mRNA-1:exon:13300;Parent=Msa1292060-mRNA-1
chr8_3	five_prime_UTR	47979892	47980261	47979892	ID=Msa1292060-mRNA-1:five_prime_utr;Parent=Msa1292060-mRNA-1
chr8_3	CDS	47977601	47977992	47977601	ID=Msa1292060-mRNA-1:cds;Parent=Msa1292060-mRNA-1
chr8_3	CDS	47976344	47976845	47976344	ID=Msa1292060-mRNA-1:cds;Parent=Msa1292060-mRNA-1
chr8_3	three_prime_UTR	47976019	47976343	47976019	ID=Msa1292060-mRNA-1:three_prime_utr;Parent=Msa1292060-mRNA-1

Gene Sequence

>Msa1292060

ATGGGAGACGTGGCTAAGGACCTAGCAGCCGGTACCATTGGAGGGGCTGCGCAATTGATAGTTGGACATCCCTTTGACACCATCAAGGTCAAGCTCCAAAGCCAACCTACACCACTCCCTGGTCAGCCTCCCAGGTATTCTGGTGCATTTGATGCCGTCAGGCAGACGTTAGCGGCCGAAGGTCCAAGGGGTTTATACAAAGGTATGGGCGCCCCGCTTGCTACGGTTGCAGCCTTCAATGCTGTCCTGTTTACAGTTAGGGGACAAATGGAATCATTAGTGAGGTCCCATCCTGGTGCTCACCTCACAGTGAATCAACAGTTTGTTTGTGGAGCTGGAGCTGGTGTCGCTGTTTCCTTTCTTGCTTGTCCAACTGAATTAATCAAATGCAGGTTGCAAGCACAAAGTGCGCTTGCTGGCACTGGAACAGCAGCTGTGGCTGTTAAATATGGAGGACCAATGGATGTGACCCGACAAGTTCTCCGATCAGAGGGGGGCGTGAGAGGTCTTTTCAAAGGCTTACTTCCCACAATGGGACGCGAGATACCTGGAAACGCGATAATGTTTGGTGTATATGAAGCAATAAAACAACAGTTTGCAGGCGGCACCGATACCTCTGGCCTAAGCCAAGGTTCTCTGATTGTTGCTGGAGGCTTGGCTGGTGCTTCTTTCTGGGGCATCGTTTACCCAACTGATGTTATTAAAAGTGTGCTTCAAGTAGATGATTATAAGAACCCTAAGTATTCTGGTTCATTTGATGCTTTCAGAAAGATTAAAGCCACTGAGGGATTCAAAGGCCTATACAAGGGTTTTGGTCCTGCAATGGGCAGAAGTGTTCCTGCAAATGCAGCATGCTTCTTGGCATATGAGATGACAAGATCAGCTTTAGGATGA

Protein sequence

>Msa1292060

MGDVAKDLAAGTIGGAAQLIVGHPFDTIKVKLQSQPTPLPGQPPRYSGAFDAVRQTLAAEGPRGLYKGMGAPLATVAAFNAVLFTVRGQMESLVRSHPGAHLTVNQQFVCGAGAGVAVSFLACPTELIKCRLQAQSALAGTGTAAVAVKYGGPMDVTRQVLRSEGGVRGLFKGLLPTMGREIPGNAIMFGVYEAIKQQFAGGTDTSGLSQGSLIVAGGLAGASFWGIVYPTDVIKSVLQVDDYKNPKYSGSFDAFRKIKATEGFKGLYKGFGPAMGRSVPANAACFLAYEMTRSALG*