AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0691350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0691350 MtrunA17_Chr4g0040481 90.164 427 31 4 1 419 1 424 0.0 706
Msa0691350 MtrunA17_Chr2g0324891 63.073 436 127 19 1 416 1 422 2.00e-157 451
Msa0691350 MtrunA17_Chr5g0400241 34.868 304 145 10 121 410 267 531 3.03e-43 159
Msa0691350 MtrunA17_Chr4g0054981 36.559 279 141 11 145 416 299 548 8.21e-42 155
Msa0691350 MtrunA17_Chr4g0009851 38.710 93 56 1 120 212 134 225 6.64e-18 84.0
Msa0691350 MtrunA17_Chr3g0137101 47.368 76 40 0 148 223 190 265 2.07e-17 84.3
Msa0691350 MtrunA17_Chr1g0161431 36.567 134 73 2 81 214 137 258 5.20e-17 83.2
Msa0691350 MtrunA17_Chr1g0149841 54.545 66 30 0 153 218 188 253 2.72e-16 80.9
Msa0691350 MtrunA17_Chr2g0304841 54.839 62 28 0 151 212 208 269 5.25e-16 80.1
Msa0691350 MtrunA17_Chr4g0067981 56.667 60 26 0 155 214 214 273 6.46e-16 80.1
Msa0691350 MtrunA17_Chr4g0009981 38.202 89 54 1 124 212 85 172 1.16e-15 76.6
Msa0691350 MtrunA17_Chr2g0294911 40.230 87 47 1 155 236 210 296 7.95e-15 76.6
Msa0691350 MtrunA17_Chr7g0255711 44.872 78 37 1 137 214 78 149 8.19e-15 74.7
Msa0691350 MtrunA17_Chr4g0009901 38.710 93 56 1 120 212 133 224 1.32e-14 73.6
Msa0691350 MtrunA17_Chr8g0376881 47.826 69 36 0 155 223 208 276 2.77e-14 74.7
Msa0691350 MtrunA17_Chr4g0031961 39.506 81 49 0 143 223 118 198 5.33e-14 72.4
Msa0691350 MtrunA17_Chr7g0275401 36.066 122 71 2 98 212 127 248 2.10e-13 70.5
Msa0691350 MtrunA17_Chr7g0275891 32.847 137 56 4 151 255 186 318 2.45e-13 70.9
Msa0691350 MtrunA17_Chr3g0123131 44.928 69 38 0 155 223 198 266 3.01e-13 71.6
Msa0691350 MtrunA17_Chr4g0062921 36.000 125 60 4 108 212 168 292 4.26e-13 70.5
Msa0691350 MtrunA17_Chr6g0461641 56.364 55 23 1 158 211 44 98 1.15e-12 69.3
Msa0691350 MtrunA17_Chr4g0074401 49.091 55 28 0 154 208 201 255 2.34e-12 68.9
Msa0691350 MtrunA17_Chr4g0045361 41.573 89 47 2 152 239 270 354 4.71e-12 67.4
Msa0691350 MtrunA17_Chr6g0473731 44.118 68 38 0 155 222 94 161 5.24e-12 67.4
Msa0691350 MtrunA17_Chr2g0321461 55.769 52 22 1 158 208 47 98 5.76e-12 67.0
Msa0691350 MtrunA17_Chr1g0198601 36.275 102 52 2 147 235 202 303 8.17e-12 66.2
Msa0691350 MtrunA17_Chr1g0149831 46.970 66 32 1 153 218 125 187 8.86e-12 64.7
Msa0691350 MtrunA17_Chr3g0134481 48.936 47 24 0 161 207 137 183 1.16e-11 63.5
Msa0691350 MtrunA17_Chr4g0074411 49.091 55 28 0 154 208 199 253 1.23e-11 66.6
Msa0691350 MtrunA17_Chr1g0175081 50.000 52 25 1 158 208 24 75 1.94e-11 60.5
Msa0691350 MtrunA17_Chr4g0009991 39.744 78 46 1 128 205 40 116 2.92e-11 60.8
Msa0691350 MtrunA17_Chr1g0149851 42.667 75 28 1 153 212 188 262 3.23e-11 65.1
Msa0691350 MtrunA17_Chr5g0417521 44.000 75 36 3 158 231 17 86 3.40e-11 64.3
Msa0691350 MtrunA17_Chr7g0254981 47.222 72 34 2 141 208 32 103 6.38e-11 62.8
Msa0691350 MtrunA17_Chr1g0190301 46.377 69 34 2 144 211 165 231 7.98e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0691350 AT2G20570.1 45.916 453 176 21 1 418 1 419 2.99e-83 261
Msa0691350 AT2G20570.2 44.350 469 176 22 1 418 1 435 3.89e-79 251
Msa0691350 AT5G44190.1 41.591 440 176 15 1 415 1 384 3.32e-72 232
Msa0691350 AT5G44190.2 43.750 288 123 9 1 272 1 265 5.10e-60 199
Msa0691350 AT2G20570.3 36.865 453 170 22 1 418 1 372 1.38e-40 148
Msa0691350 AT4G18020.3 32.512 406 180 12 37 418 191 526 1.79e-34 134
Msa0691350 AT4G18020.2 32.512 406 180 12 37 418 191 526 1.79e-34 134
Msa0691350 AT4G18020.1 32.512 406 180 12 37 418 191 526 1.79e-34 134
Msa0691350 AT4G18020.6 32.512 406 180 12 37 418 191 526 1.79e-34 134
Msa0691350 AT4G18020.9 32.512 406 180 12 37 418 191 526 1.79e-34 134
Msa0691350 AT4G18020.8 35.510 245 132 6 37 271 191 419 1.94e-29 120
Msa0691350 AT4G18020.11 35.510 245 132 6 37 271 191 419 1.94e-29 120
Msa0691350 AT4G18020.4 35.510 245 132 6 37 271 191 419 1.94e-29 120
Msa0691350 AT4G18020.5 35.510 245 132 6 37 271 191 419 1.94e-29 120
Msa0691350 AT4G18020.10 35.510 245 132 6 37 271 191 419 1.94e-29 120
Msa0691350 AT4G18020.7 35.510 245 132 6 37 271 191 419 1.94e-29 120
Msa0691350 AT5G58080.1 55.738 61 27 0 154 214 192 252 3.06e-16 81.3
Msa0691350 AT2G01760.3 37.209 129 75 3 151 277 130 254 3.77e-16 79.3
Msa0691350 AT2G01760.2 37.209 129 75 3 151 277 223 347 4.08e-16 80.1
Msa0691350 AT2G01760.1 37.209 129 75 3 151 277 195 319 4.11e-16 80.1
Msa0691350 AT3G16857.3 44.565 92 38 1 136 214 134 225 8.96e-16 79.7
Msa0691350 AT3G16857.2 44.565 92 38 1 136 214 204 295 1.14e-15 79.7
Msa0691350 AT3G16857.1 44.565 92 38 1 136 214 204 295 1.23e-15 79.3
Msa0691350 AT3G10760.1 51.667 60 29 0 155 214 104 163 3.56e-15 76.6
Msa0691350 AT5G05090.1 51.667 60 29 0 155 214 80 139 4.33e-15 75.5
Msa0691350 AT5G59570.2 41.892 74 43 0 158 231 143 216 9.28e-15 75.1
Msa0691350 AT5G59570.1 41.892 74 43 0 158 231 143 216 9.28e-15 75.1
Msa0691350 AT2G25180.1 50.000 62 31 0 153 214 192 253 1.39e-14 76.3
Msa0691350 AT4G31920.1 52.542 59 28 0 150 208 177 235 1.63e-14 75.9
Msa0691350 AT3G46640.2 46.479 71 36 1 155 223 143 213 2.26e-14 73.9
Msa0691350 AT3G46640.1 46.479 71 36 1 155 223 143 213 2.26e-14 73.9
Msa0691350 AT3G46640.3 46.479 71 36 1 155 223 143 213 4.37e-14 73.2
Msa0691350 AT5G07210.1 36.800 125 72 2 100 224 177 294 5.23e-14 74.3
Msa0691350 AT5G07210.2 36.800 125 72 2 100 224 169 286 5.37e-14 74.3
Msa0691350 AT2G40970.1 50.877 57 28 0 158 214 107 163 8.71e-14 71.2
Msa0691350 AT2G03500.1 37.398 123 67 2 148 260 225 347 1.34e-13 72.8
Msa0691350 AT2G27070.1 37.069 116 70 1 100 215 172 284 1.50e-13 72.8
Msa0691350 AT1G13300.1 43.333 90 47 3 124 212 152 238 1.04e-12 69.3
Msa0691350 AT3G04030.1 57.407 54 22 1 156 208 45 98 1.48e-12 69.3
Msa0691350 AT3G04030.3 57.407 54 22 1 156 208 45 98 1.63e-12 68.9
Msa0691350 AT5G18240.2 59.259 54 21 1 156 208 45 98 2.26e-12 68.6
Msa0691350 AT5G18240.3 59.259 54 21 1 156 208 45 98 2.26e-12 68.6
Msa0691350 AT5G18240.5 59.259 54 21 1 156 208 45 98 2.56e-12 68.6
Msa0691350 AT5G18240.1 59.259 54 21 1 156 208 45 98 2.75e-12 68.6
Msa0691350 AT5G18240.4 59.259 54 21 1 156 208 45 98 2.75e-12 68.6
Msa0691350 AT1G68670.1 37.037 108 63 2 134 236 193 300 9.66e-12 66.6
Msa0691350 AT1G67710.2 33.884 121 70 2 104 214 67 187 1.42e-11 66.6
Msa0691350 AT1G67710.3 33.884 121 70 2 104 214 58 178 1.86e-11 66.2
Msa0691350 AT1G67710.1 33.884 121 70 2 104 214 132 252 2.60e-11 65.9
Msa0691350 AT3G04030.2 52.830 53 25 0 156 208 45 97 2.62e-11 65.5
Msa0691350 AT5G45580.1 53.846 52 23 1 158 208 9 60 7.19e-11 62.8
Msa0691350 AT1G25550.1 41.892 74 42 1 141 213 194 267 8.12e-11 63.5

Find 157 sgRNAs with CRISPR-Local

Find 275 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCGGTGCCTTCAAGGATTT+TGG 0.156203 4_4:+59433774 Msa0691350:CDS
CCTTTCCGAAGGAAATTTAT+TGG 0.220430 4_4:+59432617 Msa0691350:CDS
GGCCGGATCATTGCGTTTAA+TGG 0.260515 4_4:-59435778 None:intergenic
TGGTGAGGAGGAGGAGGATG+AGG 0.282598 4_4:-59434204 None:intergenic
TATGGGATTAGTAAATAAAA+TGG 0.295355 4_4:+59436863 Msa0691350:three_prime_UTR
ATGCCATGGGGTGGGATGCC+GGG 0.306670 4_4:-59436111 None:intergenic
TGGAGACATCTTACCGGATT+TGG 0.308487 4_4:+59432683 Msa0691350:CDS
GGATGAGAAATCGTCGAATT+AGG 0.310005 4_4:-59436687 None:intergenic
TATGTACGATTGGTATAGTA+TGG 0.317826 4_4:+59436845 Msa0691350:three_prime_UTR
CCCATTTGATTATTATTAGA+AGG 0.319606 4_4:+59432452 Msa0691350:five_prime_UTR
AAACCCATGGTGGGTGCAGC+TGG 0.319978 4_4:-59434177 None:intergenic
AATCGTACATATATACAAAA+TGG 0.337228 4_4:-59436833 None:intergenic
ATGGGGACATCCTTCAAATA+TGG 0.339407 4_4:+59434245 Msa0691350:CDS
AAAACTAATTAATCCTTTAC+CGG 0.340492 4_4:+59436640 Msa0691350:three_prime_UTR
ATACATGTAAGGGTCTAAAA+TGG 0.354016 4_4:-59434224 None:intergenic
CATGCCATGGGGTGGGATGC+CGG 0.361244 4_4:-59436112 None:intergenic
AACTTCGACGACTTCTTTGT+TGG 0.363264 4_4:+59432648 Msa0691350:CDS
AGATGCAATGCAACATCTAA+TGG 0.367911 4_4:-59436708 None:intergenic
TGGGGTGGGATGCCGGGGAT+TGG 0.376158 4_4:-59436105 None:intergenic
ACCTTCTAATAATAATCAAA+TGG 0.380751 4_4:-59432453 None:intergenic
TGAGGAGGAGGAGGATGAGG+GGG 0.386773 4_4:-59434201 None:intergenic
ACAAATCTCCTGTGCAAGTC+TGG 0.392453 4_4:-59433721 None:intergenic
GATGAATTCCTCGGCGTCAT+TGG 0.395120 4_4:+59432761 Msa0691350:CDS
GTGAGGAGGAGGAGGATGAG+GGG 0.396462 4_4:-59434202 None:intergenic
TGAGGATAAAACAAGTTGTT+CGG 0.396562 4_4:+59432827 Msa0691350:CDS
GAGAACGTGTGTTCAAATAA+TGG 0.401910 4_4:-59432313 None:intergenic
CCAGCACTTGATAGCGTAAT+GGG 0.402444 4_4:+59436563 Msa0691350:CDS
GAGGAGGAGGAGGATGAGGG+GGG 0.407806 4_4:-59434200 None:intergenic
TTAAAAGATGCAGGTGGAAA+AGG 0.411700 4_4:+59432909 Msa0691350:CDS
GAGAGCATAGACGCAGCTAT+TGG 0.425120 4_4:+59436491 Msa0691350:CDS
TGTTCAAATAATGGAGAGTT+AGG 0.425347 4_4:-59432304 None:intergenic
CATCTTACCGGATTTGGTAA+TGG 0.436295 4_4:+59432689 Msa0691350:CDS
TAACATTGCTAGTCACCTTC+AGG 0.436461 4_4:+59433828 Msa0691350:CDS
CCTTTACCGGGGAGCTAGCT+AGG 0.438569 4_4:+59436653 Msa0691350:three_prime_UTR
ATAATCTCCAAAATCCTTGA+AGG 0.440155 4_4:-59433781 None:intergenic
GAGAACTCAGCAAACATCTC+TGG 0.443524 4_4:-59432714 None:intergenic
GGTGAGGAGGAGGAGGATGA+GGG 0.445969 4_4:-59434203 None:intergenic
ACCACTGTTGTCAGAGGTGG+TGG 0.450953 4_4:-59435817 None:intergenic
TGGACAAGGCGGTGCCTTCA+AGG 0.452098 4_4:+59433767 Msa0691350:CDS
TCTGGATCCATTACCAAATC+CGG 0.453183 4_4:-59432696 None:intergenic
ACAATTACAACGGGCGACTT+CGG 0.456230 4_4:+59432591 Msa0691350:CDS
GGTCTAAAATGGTGAGGAGG+AGG 0.456630 4_4:-59434213 None:intergenic
GGTGGGAAACAATGTGTTCC+TGG 0.458402 4_4:-59435799 None:intergenic
ATGGTGGGTGCAGCTGGCCA+TGG 0.458606 4_4:-59434171 None:intergenic
GCTTTCCAATAAATTTCCTT+CGG 0.459139 4_4:-59432622 None:intergenic
ATGGGATTAGTAAATAAAAT+GGG 0.460142 4_4:+59436864 Msa0691350:three_prime_UTR
CTCCATTAAACGCAATGATC+CGG 0.461667 4_4:+59435776 Msa0691350:CDS
CTAAAATGGTGAGGAGGAGG+AGG 0.462304 4_4:-59434210 None:intergenic
TCGAAGAAGTGACCGGGAGC+AGG 0.463586 4_4:-59436165 None:intergenic
CCTTCTAATAATAATCAAAT+GGG 0.463808 4_4:-59432452 None:intergenic
CATGAGAGTGGCTGCGGTGT+AGG 0.464099 4_4:-59434291 None:intergenic
AATCAAAGGAGACAAGTATA+TGG 0.464153 4_4:+59434117 Msa0691350:CDS
GAAGGTGACTAGCAATGTTA+TGG 0.464906 4_4:-59433825 None:intergenic
TGCCATGGGGTGGGATGCCG+GGG 0.471359 4_4:-59436110 None:intergenic
AAGCAGCAAGTTGGAATCAA+AGG 0.471928 4_4:+59434103 Msa0691350:CDS
GCTGAGTTCTCCGTTAGTTA+CGG 0.472120 4_4:+59432726 Msa0691350:CDS
GAGGATAAAACAAGTTGTTC+GGG 0.474904 4_4:+59432828 Msa0691350:CDS
ATGGCCAGCTGCACCCACCA+TGG 0.477260 4_4:+59434173 Msa0691350:CDS
TGTTTATTGTGCAGGTGGAT+TGG 0.477271 4_4:+59433695 Msa0691350:intron
GTTGCACTCAAGCTTAGAAA+TGG 0.477328 4_4:-59432374 None:intergenic
AGACAAGTATATGGTGGTGT+AGG 0.478471 4_4:+59434126 Msa0691350:CDS
GACAAGTATATGGTGGTGTA+GGG 0.478876 4_4:+59434127 Msa0691350:CDS
TGGCCAGCTGCACCCACCAT+GGG 0.485571 4_4:+59434174 Msa0691350:CDS
CCTAGCTAGCTCCCCGGTAA+AGG 0.488512 4_4:-59436653 None:intergenic
CCATGGTTGCCACTACCACT+TGG 0.489750 4_4:+59436530 Msa0691350:CDS
AACATGCAGAGACCATCATT+TGG 0.489976 4_4:+59436078 Msa0691350:intron
CGTCGATGGAGACATCTTAC+CGG 0.493741 4_4:+59432677 Msa0691350:CDS
GATCTGCTTTACACATGCCA+TGG 0.496492 4_4:-59436125 None:intergenic
TCTTTGTTAGTGTTCCTCAA+TGG 0.497869 4_4:-59432525 None:intergenic
TCCAGCACTTGATAGCGTAA+TGG 0.499694 4_4:+59436562 Msa0691350:CDS
ATTAGCACCCTTCCTGCTCC+CGG 0.500715 4_4:+59436153 Msa0691350:CDS
GGGAGTACTACAATTACAAC+GGG 0.501212 4_4:+59432582 Msa0691350:CDS
AGGATGTCCCCATACATGTA+AGG 0.503004 4_4:-59434235 None:intergenic
GAAGTGACCGGGAGCAGGAA+GGG 0.509953 4_4:-59436160 None:intergenic
CCAATAAATTTCCTTCGGAA+AGG 0.511123 4_4:-59432617 None:intergenic
TTGAGGAACACTAACAAAGA+TGG 0.514130 4_4:+59432528 Msa0691350:CDS
CCCATTACGCTATCAAGTGC+TGG 0.514753 4_4:-59436563 None:intergenic
ATCTGCTTTACACATGCCAT+GGG 0.515933 4_4:-59436124 None:intergenic
TGATTCCTCGCCGTAACTAA+CGG 0.519226 4_4:-59432736 None:intergenic
TTTGTTGGCATCAACGTCGA+TGG 0.522723 4_4:+59432663 Msa0691350:CDS
GAAGGATCTTGAGGTGGTGG+AGG 0.522935 4_4:-59434330 None:intergenic
AATGAAGGATCTTGAGGTGG+TGG 0.528793 4_4:-59434333 None:intergenic
GCTTTGAGTCCAAGTGGTAG+TGG 0.528879 4_4:-59436539 None:intergenic
AGATGGAAACCAAGAAGAGA+TGG 0.529997 4_4:+59432545 Msa0691350:CDS
AAAGACTGGTCCATATTTGA+AGG 0.536097 4_4:-59434255 None:intergenic
GGAAACAATGTGTTCCTGGC+CGG 0.538523 4_4:-59435795 None:intergenic
TTTCTTCAAGTGCATGATGA+GGG 0.538544 4_4:-59436620 None:intergenic
AAAACAAGTTGTTCGGGTCA+AGG 0.542391 4_4:+59432834 Msa0691350:CDS
TGGGAAGTCGAAGAAGTGAC+CGG 0.545060 4_4:-59436172 None:intergenic
AATCCATTGTTAAAAGATGC+AGG 0.548676 4_4:+59432900 Msa0691350:CDS
GGGATTGGACATCCAAATGA+TGG 0.551089 4_4:-59436090 None:intergenic
AGTAAATCTAAGAATCCTCA+AGG 0.553031 4_4:+59432945 Msa0691350:CDS
CTACACCGCAGCCACTCTCA+TGG 0.554231 4_4:+59434292 Msa0691350:CDS
TTGCCATTCTCATCACCAGC+AGG 0.556256 4_4:+59434356 Msa0691350:CDS
CTACCTGCTGGTGATGAGAA+TGG 0.556903 4_4:-59434359 None:intergenic
GCTTTACACATGCCATGGGG+TGG 0.557961 4_4:-59436120 None:intergenic
ACTCAAGCTTAGAAATGGTA+GGG 0.565017 4_4:-59432369 None:intergenic
AAGTTGTTCGGGTCAAGGCT+CGG 0.571650 4_4:+59432839 Msa0691350:CDS
AAACTAATTAATCCTTTACC+GGG 0.572727 4_4:+59436641 Msa0691350:three_prime_UTR
CACTCAAGCTTAGAAATGGT+AGG 0.573776 4_4:-59432370 None:intergenic
CTTCAAGTGCATGATGAGGG+TGG 0.573822 4_4:-59436617 None:intergenic
GAAACTCAATATATAAAACT+AGG 0.576041 4_4:-59432430 None:intergenic
TCATTTGGATGTCCAATCCC+CGG 0.576190 4_4:+59436093 Msa0691350:CDS
CTTTACACATGCCATGGGGT+GGG 0.577896 4_4:-59436119 None:intergenic
ATCCCCGGCATCCCACCCCA+TGG 0.583757 4_4:+59436108 Msa0691350:CDS
GTGCAAGCTGTGGAACAGCT+AGG 0.587705 4_4:+59433742 Msa0691350:CDS
TTAAACGCAATGATCCGGCC+AGG 0.588149 4_4:+59435781 Msa0691350:CDS
GTGAAGCAGAAGCAGCAAGT+TGG 0.590428 4_4:+59434094 Msa0691350:CDS
GGATGTCCCCATACATGTAA+GGG 0.590827 4_4:-59434234 None:intergenic
CCAAGTGGTAGTGGCAACCA+TGG 0.591902 4_4:-59436530 None:intergenic
GGTGTCCATGAGAGTGGCTG+CGG 0.595402 4_4:-59434297 None:intergenic
ATGTACGATTGGTATAGTAT+GGG 0.596341 4_4:+59436846 Msa0691350:three_prime_UTR
ATTGGACACCAGACTTGCAC+AGG 0.600253 4_4:+59433713 Msa0691350:CDS
GAAGGTTGAGTACTTGAGTG+TGG 0.600374 4_4:+59432470 Msa0691350:five_prime_UTR
TTTAGACCCTTACATGTATG+GGG 0.601372 4_4:+59434228 Msa0691350:CDS
TCACTTCTTCGACTTCCCAC+CGG 0.602135 4_4:+59436176 Msa0691350:CDS
GAATTCAAAGAAAGATTGTG+AGG 0.605803 4_4:+59432809 Msa0691350:CDS
TGATGAGAATGGCAAAATGA+AGG 0.608343 4_4:-59434348 None:intergenic
TGGCAAAATGAAGGATCTTG+AGG 0.610895 4_4:-59434339 None:intergenic
GCCACTCTCATGGACACCAT+CGG 0.612134 4_4:+59434302 Msa0691350:CDS
AGAAGTGACCGGGAGCAGGA+AGG 0.612201 4_4:-59436161 None:intergenic
GACTTCGGAGACCTTTCCGA+AGG 0.616050 4_4:+59432606 Msa0691350:CDS
GGAATCAGAGATGAATTCCT+CGG 0.620947 4_4:+59432752 Msa0691350:CDS
GCCACATGTGCAGAAAAGAC+TGG 0.621691 4_4:-59434269 None:intergenic
AATGCTCAAGCATTAGCGCA+TGG 0.622785 4_4:-59436808 None:intergenic
AGCTGTGGAACAGCTAGGAG+TGG 0.623334 4_4:+59433747 Msa0691350:CDS
AGAGAGAAGTGATCAGTCCA+TGG 0.624850 4_4:+59434154 Msa0691350:CDS
TCAAGGGAAGAGAAAAGTCA+AGG 0.631578 4_4:+59432962 Msa0691350:CDS
GGCATGTGTAAAGCAGATCA+CGG 0.631742 4_4:+59436129 Msa0691350:CDS
ATTGGAGAACACCAGCAAAG+TGG 0.632108 4_4:+59432779 Msa0691350:CDS
GGGAAGTCGAAGAAGTGACC+GGG 0.633060 4_4:-59436171 None:intergenic
AAGGGTCTAAAATGGTGAGG+AGG 0.633488 4_4:-59434216 None:intergenic
TGCTTGCAGTGTCACCATTG+AGG 0.634204 4_4:+59432511 Msa0691350:CDS
CAGGAGATTTGTGCAAGCTG+TGG 0.634615 4_4:+59433732 Msa0691350:CDS
ACAAGTATATGGTGGTGTAG+GGG 0.636855 4_4:+59434128 Msa0691350:CDS
ACCGATGGTGTCCATGAGAG+TGG 0.639166 4_4:-59434303 None:intergenic
GTTACCACTGTTGTCAGAGG+TGG 0.641294 4_4:-59435820 None:intergenic
CAAAGGAGACAAGTATATGG+TGG 0.641945 4_4:+59434120 Msa0691350:CDS
TTGAAACCTAGCTAGCTCCC+CGG 0.643660 4_4:-59436659 None:intergenic
TGTAAGGGTCTAAAATGGTG+AGG 0.653242 4_4:-59434219 None:intergenic
GTAAATCTAAGAATCCTCAA+GGG 0.653344 4_4:+59432946 Msa0691350:CDS
ATAGTTACCACTGTTGTCAG+AGG 0.658785 4_4:-59435823 None:intergenic
GGAACAGCTAGGAGTGGACA+AGG 0.659903 4_4:+59433753 Msa0691350:CDS
AACTAATTAATCCTTTACCG+GGG 0.665216 4_4:+59436642 Msa0691350:three_prime_UTR
GGGGAGTACTACAATTACAA+CGG 0.686337 4_4:+59432581 Msa0691350:CDS
GGTGGTGGAGGAGCTACCGA+TGG 0.688749 4_4:-59434318 None:intergenic
GAGTAAAAGAGATGAATCTG+TGG 0.689938 4_4:+59432872 Msa0691350:CDS
GCTGGAGCTTTGAGTCCAAG+TGG 0.690127 4_4:-59436545 None:intergenic
GTTCTCCGTTAGTTACGGCG+AGG 0.691247 4_4:+59432731 Msa0691350:CDS
GGTGTTCTCCAATGACGCCG+AGG 0.692927 4_4:-59432769 None:intergenic
ATGGGTGAACTACAAAGACA+AGG 0.697859 4_4:+59436581 Msa0691350:CDS
CCACTGTTGTCAGAGGTGGT+GGG 0.703169 4_4:-59435816 None:intergenic
CAAAATGAAGGATCTTGAGG+TGG 0.718294 4_4:-59434336 None:intergenic
CCCACCACCTCTGACAACAG+TGG 0.721504 4_4:+59435816 Msa0691350:CDS
CCATTGTTAAAAGATGCAGG+TGG 0.735818 4_4:+59432903 Msa0691350:CDS
TCTGCTTTACACATGCCATG+GGG 0.759671 4_4:-59436123 None:intergenic
ACAGCTAGGAGTGGACAAGG+CGG 0.769326 4_4:+59433756 Msa0691350:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAACAAATTATTGTTT+AGG + chr4_4:59434551-59434570 Msa0691350:intron 10.0%
!!! AAAAATTTTATATATGAGAT+CGG + chr4_4:59434644-59434663 Msa0691350:intron 10.0%
!!! TCTCTTTTAATATTTTTTTT+GGG - chr4_4:59434759-59434778 None:intergenic 10.0%
!!! TTCTCTTTTAATATTTTTTT+TGG - chr4_4:59434760-59434779 None:intergenic 10.0%
!! AACCTTAAATAAATATTATT+TGG - chr4_4:59434855-59434874 None:intergenic 10.0%
!! ATAATAACTAAATATCATTT+GGG - chr4_4:59435190-59435209 None:intergenic 10.0%
!! TATAATAACTAAATATCATT+TGG - chr4_4:59435191-59435210 None:intergenic 10.0%
!! TTTGTCATATATTATATATA+AGG + chr4_4:59435352-59435371 Msa0691350:intron 10.0%
!!! CAAATTTTTTTTTTTTTTGA+GGG - chr4_4:59435500-59435519 None:intergenic 10.0%
!!! TCAAATTTTTTTTTTTTTTG+AGG - chr4_4:59435501-59435520 None:intergenic 10.0%
!!! CTTTTTATAGCATTTTTTTT+CGG + chr4_4:59433317-59433336 Msa0691350:intron 15.0%
!! TTACTTATATATAAATTGTG+AGG - chr4_4:59433465-59433484 None:intergenic 15.0%
!!! TTTTATTATTGCAGAAATAT+AGG + chr4_4:59434046-59434065 Msa0691350:intron 15.0%
!! TATACAATGATAGATATATA+GGG - chr4_4:59434418-59434437 None:intergenic 15.0%
!!! ACAATTAAGATTTTAAACTT+TGG + chr4_4:59434478-59434497 Msa0691350:intron 15.0%
!!! TAGGAAAATATACAATTTTT+AGG + chr4_4:59434680-59434699 Msa0691350:intron 15.0%
!! GTCCAAATAATATTTATTTA+AGG + chr4_4:59434850-59434869 Msa0691350:intron 15.0%
!! CAAATGGTATATATAAAAAT+TGG - chr4_4:59434915-59434934 None:intergenic 15.0%
!!! TTGTTTTAAAATCTTTTTCT+AGG - chr4_4:59435416-59435435 None:intergenic 15.0%
!!! ATGTGAAACTTTTTAATATA+TGG - chr4_4:59436280-59436299 None:intergenic 15.0%
!!! CATTTTTTTTTTCTTTCATT+TGG - chr4_4:59436782-59436801 None:intergenic 15.0%
!!! ATAATAAGATAGTTTTGTGA+AGG - chr4_4:59432344-59432363 None:intergenic 20.0%
!!! ACTAGGTGAATTATTATTTT+TGG - chr4_4:59432416-59432435 None:intergenic 20.0%
!! GAAACTCAATATATAAAACT+AGG - chr4_4:59432433-59432452 None:intergenic 20.0%
!! CCTTCTAATAATAATCAAAT+GGG - chr4_4:59432455-59432474 None:intergenic 20.0%
!! ACCTTCTAATAATAATCAAA+TGG - chr4_4:59432456-59432475 None:intergenic 20.0%
!! TATTAAAAAAATGTTAGCAG+TGG + chr4_4:59433282-59433301 Msa0691350:intron 20.0%
!! TGTTAACAATTCAAATTTAC+CGG + chr4_4:59433343-59433362 Msa0691350:intron 20.0%
!! ATATAAATTGTGAGGATATT+TGG - chr4_4:59433457-59433476 None:intergenic 20.0%
!! CACTGCTATTATTATTAAAT+AGG + chr4_4:59433485-59433504 Msa0691350:intron 20.0%
!! CTATACAATGATAGATATAT+AGG - chr4_4:59434419-59434438 None:intergenic 20.0%
!! GTACAAACTGAAATAATTAA+GGG - chr4_4:59434514-59434533 None:intergenic 20.0%
!! AGTACAAACTGAAATAATTA+AGG - chr4_4:59434515-59434534 None:intergenic 20.0%
!!! GAAAATATACAATTTTTAGG+AGG + chr4_4:59434683-59434702 Msa0691350:intron 20.0%
!!! TTTTGAATATAACGAACAAA+TGG - chr4_4:59434931-59434950 None:intergenic 20.0%
!!! TTTGTCGACTTTTATATTTT+AGG + chr4_4:59435023-59435042 Msa0691350:intron 20.0%
!! TTGTTATAAATCAAAATTCG+AGG + chr4_4:59435621-59435640 Msa0691350:intron 20.0%
!!! ATTACATCACTTTACTATTT+TGG + chr4_4:59435848-59435867 Msa0691350:intron 20.0%
!!! TGTCAATCTTATCTTTAAAA+AGG - chr4_4:59435910-59435929 None:intergenic 20.0%
!! TGTGCTATATTTATATATGT+TGG - chr4_4:59435962-59435981 None:intergenic 20.0%
!! TTATTCTCCAAATATAAGTT+TGG + chr4_4:59436032-59436051 Msa0691350:intron 20.0%
!!! TGTTATGTTATTTTCCTTTA+TGG - chr4_4:59436062-59436081 None:intergenic 20.0%
!!! AATTCTCTTTATTTAGTTTC+CGG + chr4_4:59436254-59436273 Msa0691350:intron 20.0%
!!! CTTTTAGGTGTTTTTATATT+GGG - chr4_4:59436386-59436405 None:intergenic 20.0%
!! AAAACTAATTAATCCTTTAC+CGG + chr4_4:59436640-59436659 Msa0691350:three_prime_UTR 20.0%
!! AATCGTACATATATACAAAA+TGG - chr4_4:59436836-59436855 None:intergenic 20.0%
!!! ATTTTGTATATATGTACGAT+TGG + chr4_4:59436835-59436854 Msa0691350:three_prime_UTR 20.0%
!!! TATGGGATTAGTAAATAAAA+TGG + chr4_4:59436863-59436882 Msa0691350:three_prime_UTR 20.0%
!!! ATGGGATTAGTAAATAAAAT+GGG + chr4_4:59436864-59436883 Msa0691350:three_prime_UTR 20.0%
! CCCATTTGATTATTATTAGA+AGG + chr4_4:59432452-59432471 Msa0691350:five_prime_UTR 25.0%
! AGTCAAGGTATACTTAAATA+AGG + chr4_4:59432977-59432996 Msa0691350:intron 25.0%
! CTAAGTGGTTTATCTTAATT+TGG + chr4_4:59433022-59433041 Msa0691350:intron 25.0%
! TTTGCTTATTCATCGTAATT+AGG + chr4_4:59433406-59433425 Msa0691350:intron 25.0%
! AATTTGCATATTACATGTCT+TGG + chr4_4:59433574-59433593 Msa0691350:intron 25.0%
!!! CACTATTTATCAAGTTTTGA+TGG + chr4_4:59433982-59434001 Msa0691350:intron 25.0%
! TGATAGATATATAGGGAATT+TGG - chr4_4:59434411-59434430 None:intergenic 25.0%
!! TTTTTATATATGAAACCAGC+GGG - chr4_4:59434538-59434557 None:intergenic 25.0%
!! GTTTTTATATATGAAACCAG+CGG - chr4_4:59434539-59434558 None:intergenic 25.0%
!! AATTTTATATATGAGATCGG+AGG + chr4_4:59434647-59434666 Msa0691350:intron 25.0%
!! ATTTTATATATGAGATCGGA+GGG + chr4_4:59434648-59434667 Msa0691350:intron 25.0%
!!! GTGCTCAAATATTGTTATTT+GGG - chr4_4:59434728-59434747 None:intergenic 25.0%
!! TGTGCTCAAATATTGTTATT+TGG - chr4_4:59434729-59434748 None:intergenic 25.0%
! AACAAGGATTCAATTATATG+TGG - chr4_4:59435477-59435496 None:intergenic 25.0%
!!! TTTTTTTTTTTGAGGGAACA+AGG - chr4_4:59435493-59435512 None:intergenic 25.0%
!! GAAACTTTTTAATATATGGC+CGG - chr4_4:59436276-59436295 None:intergenic 25.0%
! AAGAACATGTGTTTATAAGT+TGG + chr4_4:59436315-59436334 Msa0691350:intron 25.0%
! AGAACATGTGTTTATAAGTT+GGG + chr4_4:59436316-59436335 Msa0691350:intron 25.0%
!!! GCTTTTAGGTGTTTTTATAT+TGG - chr4_4:59436387-59436406 None:intergenic 25.0%
! AAACTAATTAATCCTTTACC+GGG + chr4_4:59436641-59436660 Msa0691350:three_prime_UTR 25.0%
! TGTTCAAATAATGGAGAGTT+AGG - chr4_4:59432307-59432326 None:intergenic 30.0%
!! TTTTGGTTGCACATTAGAAA+TGG - chr4_4:59432399-59432418 None:intergenic 30.0%
GCTTTCCAATAAATTTCCTT+CGG - chr4_4:59432625-59432644 None:intergenic 30.0%
GAATTCAAAGAAAGATTGTG+AGG + chr4_4:59432809-59432828 Msa0691350:CDS 30.0%
TGAGGATAAAACAAGTTGTT+CGG + chr4_4:59432827-59432846 Msa0691350:CDS 30.0%
AATCCATTGTTAAAAGATGC+AGG + chr4_4:59432900-59432919 Msa0691350:CDS 30.0%
AGTAAATCTAAGAATCCTCA+AGG + chr4_4:59432945-59432964 Msa0691350:CDS 30.0%
GTAAATCTAAGAATCCTCAA+GGG + chr4_4:59432946-59432965 Msa0691350:CDS 30.0%
!!! AGAGTTTTATTCTTGCTAAG+TGG + chr4_4:59433007-59433026 Msa0691350:intron 30.0%
!!! CAGTACTAACTTTTTAGCTA+AGG + chr4_4:59433080-59433099 Msa0691350:intron 30.0%
! CTGTCTCTATAATTTGTCAA+GGG - chr4_4:59433131-59433150 None:intergenic 30.0%
! TCTGTCTCTATAATTTGTCA+AGG - chr4_4:59433132-59433151 None:intergenic 30.0%
TTTATAGTGCGTTGTATGTT+TGG + chr4_4:59433205-59433224 Msa0691350:intron 30.0%
TTATCATGTGAGAAGTAAAC+CGG - chr4_4:59433365-59433384 None:intergenic 30.0%
AATTAGTGTGTTTATTGTGC+AGG + chr4_4:59433687-59433706 Msa0691350:intron 30.0%
ATAATCTCCAAAATCCTTGA+AGG - chr4_4:59433784-59433803 None:intergenic 30.0%
!! TTCAAGGATTTTGGAGATTA+TGG + chr4_4:59433783-59433802 Msa0691350:CDS 30.0%
!! TCAAGGATTTTGGAGATTAT+GGG + chr4_4:59433784-59433803 Msa0691350:CDS 30.0%
TGAAATTCATATGTACCTGA+AGG - chr4_4:59433846-59433865 None:intergenic 30.0%
AATTTCAAGAGCTAGCATAT+AGG + chr4_4:59433859-59433878 Msa0691350:intron 30.0%
AATCAAAGGAGACAAGTATA+TGG + chr4_4:59434117-59434136 Msa0691350:CDS 30.0%
ATACATGTAAGGGTCTAAAA+TGG - chr4_4:59434227-59434246 None:intergenic 30.0%
! ATTTTAGACCCTTACATGTA+TGG + chr4_4:59434226-59434245 Msa0691350:CDS 30.0%
! TTTTAGACCCTTACATGTAT+GGG + chr4_4:59434227-59434246 Msa0691350:CDS 30.0%
AAATTAGAGAAAGTCTTTCG+TGG - chr4_4:59434882-59434901 None:intergenic 30.0%
AGGATTCAATTATATGTGGT+TGG - chr4_4:59435473-59435492 None:intergenic 30.0%
! TTGTCTTCTACTCTCAATTT+TGG + chr4_4:59435882-59435901 Msa0691350:intron 30.0%
TGAATGCATGTATGTATGTA+TGG - chr4_4:59436218-59436237 None:intergenic 30.0%
! TTGAAATCTATGGAGCTTTT+AGG - chr4_4:59436401-59436420 None:intergenic 30.0%
TAAAAGCTCCATAGATTTCA+AGG + chr4_4:59436400-59436419 Msa0691350:intron 30.0%
! GAGATGTTTTATCAAAACCA+TGG + chr4_4:59436513-59436532 Msa0691350:CDS 30.0%
AACTAATTAATCCTTTACCG+GGG + chr4_4:59436642-59436661 Msa0691350:three_prime_UTR 30.0%
TATGTACGATTGGTATAGTA+TGG + chr4_4:59436845-59436864 Msa0691350:three_prime_UTR 30.0%
ATGTACGATTGGTATAGTAT+GGG + chr4_4:59436846-59436865 Msa0691350:three_prime_UTR 30.0%
GAGAACGTGTGTTCAAATAA+TGG - chr4_4:59432316-59432335 None:intergenic 35.0%
ACTCAAGCTTAGAAATGGTA+GGG - chr4_4:59432372-59432391 None:intergenic 35.0%
TCTTTGTTAGTGTTCCTCAA+TGG - chr4_4:59432528-59432547 None:intergenic 35.0%
TTGAGGAACACTAACAAAGA+TGG + chr4_4:59432528-59432547 Msa0691350:CDS 35.0%
AAAAACTCTCCATCTCTTCT+TGG - chr4_4:59432557-59432576 None:intergenic 35.0%
CCAATAAATTTCCTTCGGAA+AGG - chr4_4:59432620-59432639 None:intergenic 35.0%
CCTTTCCGAAGGAAATTTAT+TGG + chr4_4:59432617-59432636 Msa0691350:CDS 35.0%
! ATTCTTCTTTTCCACTTTGC+TGG - chr4_4:59432793-59432812 None:intergenic 35.0%
GAGGATAAAACAAGTTGTTC+GGG + chr4_4:59432828-59432847 Msa0691350:CDS 35.0%
GAGTAAAAGAGATGAATCTG+TGG + chr4_4:59432872-59432891 Msa0691350:CDS 35.0%
TTAAAAGATGCAGGTGGAAA+AGG + chr4_4:59432909-59432928 Msa0691350:CDS 35.0%
AGCTAGCATATAGGAGAAAA+AGG + chr4_4:59433868-59433887 Msa0691350:intron 35.0%
TTTAGACCCTTACATGTATG+GGG + chr4_4:59434228-59434247 Msa0691350:CDS 35.0%
! AAAGACTGGTCCATATTTGA+AGG - chr4_4:59434258-59434277 None:intergenic 35.0%
TGATGAGAATGGCAAAATGA+AGG - chr4_4:59434351-59434370 None:intergenic 35.0%
!!! AATTTGGCTGCTATTTTCAG+TGG - chr4_4:59434395-59434414 None:intergenic 35.0%
!! TTTTAGGAGGAACACTTACT+TGG + chr4_4:59434696-59434715 Msa0691350:intron 35.0%
TTTAGGAGGAACACTTACTT+GGG + chr4_4:59434697-59434716 Msa0691350:intron 35.0%
TCGATATGTACAAGTACGTT+TGG - chr4_4:59435278-59435297 None:intergenic 35.0%
!!! GTTCTTCTTCTTTTTTGACG+AGG - chr4_4:59435526-59435545 None:intergenic 35.0%
TGGTCAGCCAAACTTATATT+TGG - chr4_4:59436042-59436061 None:intergenic 35.0%
ATAAGTTTGGCTGACCATAA+AGG + chr4_4:59436045-59436064 Msa0691350:intron 35.0%
TACTCATGCCTTGAAATCTA+TGG - chr4_4:59436411-59436430 None:intergenic 35.0%
TTTCTTCAAGTGCATGATGA+GGG - chr4_4:59436623-59436642 None:intergenic 35.0%
! TTTTCTTCAAGTGCATGATG+AGG - chr4_4:59436624-59436643 None:intergenic 35.0%
AGATGCAATGCAACATCTAA+TGG - chr4_4:59436711-59436730 None:intergenic 35.0%
CACTCAAGCTTAGAAATGGT+AGG - chr4_4:59432373-59432392 None:intergenic 40.0%
GTTGCACTCAAGCTTAGAAA+TGG - chr4_4:59432377-59432396 None:intergenic 40.0%
AGATGGAAACCAAGAAGAGA+TGG + chr4_4:59432545-59432564 Msa0691350:CDS 40.0%
!! AGAGATGGAGAGTTTTTCGA+TGG + chr4_4:59432560-59432579 Msa0691350:CDS 40.0%
!! GAGATGGAGAGTTTTTCGAT+GGG + chr4_4:59432561-59432580 Msa0691350:CDS 40.0%
!! AGATGGAGAGTTTTTCGATG+GGG + chr4_4:59432562-59432581 Msa0691350:CDS 40.0%
!! GGGGAGTACTACAATTACAA+CGG + chr4_4:59432581-59432600 Msa0691350:CDS 40.0%
!! GGGAGTACTACAATTACAAC+GGG + chr4_4:59432582-59432601 Msa0691350:CDS 40.0%
AACTTCGACGACTTCTTTGT+TGG + chr4_4:59432648-59432667 Msa0691350:CDS 40.0%
! CATCTTACCGGATTTGGTAA+TGG + chr4_4:59432689-59432708 Msa0691350:CDS 40.0%
TCTGGATCCATTACCAAATC+CGG - chr4_4:59432699-59432718 None:intergenic 40.0%
GGAATCAGAGATGAATTCCT+CGG + chr4_4:59432752-59432771 Msa0691350:CDS 40.0%
AAAACAAGTTGTTCGGGTCA+AGG + chr4_4:59432834-59432853 Msa0691350:CDS 40.0%
! CCACCTGCATCTTTTAACAA+TGG - chr4_4:59432906-59432925 None:intergenic 40.0%
CCATTGTTAAAAGATGCAGG+TGG + chr4_4:59432903-59432922 Msa0691350:CDS 40.0%
! TTGACTTTTCTCTTCCCTTG+AGG - chr4_4:59432963-59432982 None:intergenic 40.0%
TCAAGGGAAGAGAAAAGTCA+AGG + chr4_4:59432962-59432981 Msa0691350:CDS 40.0%
GCAACTGTTCAAAATTTCGC+TGG + chr4_4:59433169-59433188 Msa0691350:intron 40.0%
GGATATCTTCATCTCTAACG+AGG + chr4_4:59433427-59433446 Msa0691350:intron 40.0%
!! AGTGTCTCGTGCACTTTTTA+AGG - chr4_4:59433647-59433666 None:intergenic 40.0%
TAGTGTGTTTATTGTGCAGG+TGG + chr4_4:59433690-59433709 Msa0691350:intron 40.0%
TGTTTATTGTGCAGGTGGAT+TGG + chr4_4:59433695-59433714 Msa0691350:intron 40.0%
GAAGGTGACTAGCAATGTTA+TGG - chr4_4:59433828-59433847 None:intergenic 40.0%
TAACATTGCTAGTCACCTTC+AGG + chr4_4:59433828-59433847 Msa0691350:CDS 40.0%
GTACTCGTACGATGTCTTTA+TGG + chr4_4:59433935-59433954 Msa0691350:intron 40.0%
! AAGCAGCAAGTTGGAATCAA+AGG + chr4_4:59434103-59434122 Msa0691350:CDS 40.0%
CAAAGGAGACAAGTATATGG+TGG + chr4_4:59434120-59434139 Msa0691350:CDS 40.0%
! AGACAAGTATATGGTGGTGT+AGG + chr4_4:59434126-59434145 Msa0691350:CDS 40.0%
! GACAAGTATATGGTGGTGTA+GGG + chr4_4:59434127-59434146 Msa0691350:CDS 40.0%
! ACAAGTATATGGTGGTGTAG+GGG + chr4_4:59434128-59434147 Msa0691350:CDS 40.0%
! TGTAAGGGTCTAAAATGGTG+AGG - chr4_4:59434222-59434241 None:intergenic 40.0%
ATGGGGACATCCTTCAAATA+TGG + chr4_4:59434245-59434264 Msa0691350:CDS 40.0%
CAAAATGAAGGATCTTGAGG+TGG - chr4_4:59434339-59434358 None:intergenic 40.0%
TGGCAAAATGAAGGATCTTG+AGG - chr4_4:59434342-59434361 None:intergenic 40.0%
CTCCATTAAACGCAATGATC+CGG + chr4_4:59435776-59435795 Msa0691350:CDS 40.0%
ATAGTTACCACTGTTGTCAG+AGG - chr4_4:59435826-59435845 None:intergenic 40.0%
AACATGCAGAGACCATCATT+TGG + chr4_4:59436078-59436097 Msa0691350:intron 40.0%
ATCTGCTTTACACATGCCAT+GGG - chr4_4:59436127-59436146 None:intergenic 40.0%
CATGTATGTATGTATGGCGA+AGG - chr4_4:59436212-59436231 None:intergenic 40.0%
ATGGGTGAACTACAAAGACA+AGG + chr4_4:59436581-59436600 Msa0691350:CDS 40.0%
GGATGAGAAATCGTCGAATT+AGG - chr4_4:59436690-59436709 None:intergenic 40.0%
GAAGGTTGAGTACTTGAGTG+TGG + chr4_4:59432470-59432489 Msa0691350:five_prime_UTR 45.0%
ACAATTACAACGGGCGACTT+CGG + chr4_4:59432591-59432610 Msa0691350:CDS 45.0%
TTTGTTGGCATCAACGTCGA+TGG + chr4_4:59432663-59432682 Msa0691350:CDS 45.0%
TGGAGACATCTTACCGGATT+TGG + chr4_4:59432683-59432702 Msa0691350:CDS 45.0%
GAGAACTCAGCAAACATCTC+TGG - chr4_4:59432717-59432736 None:intergenic 45.0%
GCTGAGTTCTCCGTTAGTTA+CGG + chr4_4:59432726-59432745 Msa0691350:CDS 45.0%
TGATTCCTCGCCGTAACTAA+CGG - chr4_4:59432739-59432758 None:intergenic 45.0%
ATTGGAGAACACCAGCAAAG+TGG + chr4_4:59432779-59432798 Msa0691350:CDS 45.0%
ACAAATCTCCTGTGCAAGTC+TGG - chr4_4:59433724-59433743 None:intergenic 45.0%
AGAGAGAAGTGATCAGTCCA+TGG + chr4_4:59434154-59434173 Msa0691350:CDS 45.0%
! AAGGGTCTAAAATGGTGAGG+AGG - chr4_4:59434219-59434238 None:intergenic 45.0%
GGATGTCCCCATACATGTAA+GGG - chr4_4:59434237-59434256 None:intergenic 45.0%
AGGATGTCCCCATACATGTA+AGG - chr4_4:59434238-59434257 None:intergenic 45.0%
! ACCAGTCTTTTCTGCACATG+TGG + chr4_4:59434268-59434287 Msa0691350:CDS 45.0%
AATGAAGGATCTTGAGGTGG+TGG - chr4_4:59434336-59434355 None:intergenic 45.0%
ATTTCAGTTTGTACTCCCGC+TGG + chr4_4:59434520-59434539 Msa0691350:intron 45.0%
!! GATCGGAGGGAGTATTTGAT+AGG + chr4_4:59434661-59434680 Msa0691350:intron 45.0%
AGAGTAGAAGACAACGTGCA+TGG - chr4_4:59435876-59435895 None:intergenic 45.0%
GGGATTGGACATCCAAATGA+TGG - chr4_4:59436093-59436112 None:intergenic 45.0%
TCATTTGGATGTCCAATCCC+CGG + chr4_4:59436093-59436112 Msa0691350:CDS 45.0%
TCTGCTTTACACATGCCATG+GGG - chr4_4:59436126-59436145 None:intergenic 45.0%
GATCTGCTTTACACATGCCA+TGG - chr4_4:59436128-59436147 None:intergenic 45.0%
GGCATGTGTAAAGCAGATCA+CGG + chr4_4:59436129-59436148 Msa0691350:CDS 45.0%
!! GAAGGAAATTCAGTACCGGT+GGG - chr4_4:59436194-59436213 None:intergenic 45.0%
TGGCGAAGGAAATTCAGTAC+CGG - chr4_4:59436198-59436217 None:intergenic 45.0%
!! TCCAGCACTTGATAGCGTAA+TGG + chr4_4:59436562-59436581 Msa0691350:CDS 45.0%
!! CCAGCACTTGATAGCGTAAT+GGG + chr4_4:59436563-59436582 Msa0691350:CDS 45.0%
AATGCTCAAGCATTAGCGCA+TGG - chr4_4:59436811-59436830 None:intergenic 45.0%
!!! TAATAATTAAGATTTTTTTT+TGG + chr4_4:59434445-59434464 Msa0691350:intron 5.0%
!! ATATTTAACTATTAAATTAA+AGG + chr4_4:59435252-59435271 Msa0691350:intron 5.0%
TGCTTGCAGTGTCACCATTG+AGG + chr4_4:59432511-59432530 Msa0691350:CDS 50.0%
CGTCGATGGAGACATCTTAC+CGG + chr4_4:59432677-59432696 Msa0691350:CDS 50.0%
GATGAATTCCTCGGCGTCAT+TGG + chr4_4:59432761-59432780 Msa0691350:CDS 50.0%
AAGTTGTTCGGGTCAAGGCT+CGG + chr4_4:59432839-59432858 Msa0691350:CDS 50.0%
ATTGGACACCAGACTTGCAC+AGG + chr4_4:59433713-59433732 Msa0691350:CDS 50.0%
CAGGAGATTTGTGCAAGCTG+TGG + chr4_4:59433732-59433751 Msa0691350:CDS 50.0%
GTGAAGCAGAAGCAGCAAGT+TGG + chr4_4:59434094-59434113 Msa0691350:CDS 50.0%
CTAAAATGGTGAGGAGGAGG+AGG - chr4_4:59434213-59434232 None:intergenic 50.0%
! GGTCTAAAATGGTGAGGAGG+AGG - chr4_4:59434216-59434235 None:intergenic 50.0%
GCCACATGTGCAGAAAAGAC+TGG - chr4_4:59434272-59434291 None:intergenic 50.0%
TTGCCATTCTCATCACCAGC+AGG + chr4_4:59434356-59434375 Msa0691350:CDS 50.0%
! CTACCTGCTGGTGATGAGAA+TGG - chr4_4:59434362-59434381 None:intergenic 50.0%
GGCCGGATCATTGCGTTTAA+TGG - chr4_4:59435781-59435800 None:intergenic 50.0%
TTAAACGCAATGATCCGGCC+AGG + chr4_4:59435781-59435800 Msa0691350:CDS 50.0%
GGAAACAATGTGTTCCTGGC+CGG - chr4_4:59435798-59435817 None:intergenic 50.0%
GGTGGGAAACAATGTGTTCC+TGG - chr4_4:59435802-59435821 None:intergenic 50.0%
GTTACCACTGTTGTCAGAGG+TGG - chr4_4:59435823-59435842 None:intergenic 50.0%
CTTTACACATGCCATGGGGT+GGG - chr4_4:59436122-59436141 None:intergenic 50.0%
TGGGAAGTCGAAGAAGTGAC+CGG - chr4_4:59436175-59436194 None:intergenic 50.0%
TCACTTCTTCGACTTCCCAC+CGG + chr4_4:59436176-59436195 Msa0691350:CDS 50.0%
! CGAAGGAAATTCAGTACCGG+TGG - chr4_4:59436195-59436214 None:intergenic 50.0%
! GAGAGCATAGACGCAGCTAT+TGG + chr4_4:59436491-59436510 Msa0691350:CDS 50.0%
! GCTTTGAGTCCAAGTGGTAG+TGG - chr4_4:59436542-59436561 None:intergenic 50.0%
CCCATTACGCTATCAAGTGC+TGG - chr4_4:59436566-59436585 None:intergenic 50.0%
CTTCAAGTGCATGATGAGGG+TGG - chr4_4:59436620-59436639 None:intergenic 50.0%
TTGAAACCTAGCTAGCTCCC+CGG - chr4_4:59436662-59436681 None:intergenic 50.0%
GACTTCGGAGACCTTTCCGA+AGG + chr4_4:59432606-59432625 Msa0691350:CDS 55.0%
GTTCTCCGTTAGTTACGGCG+AGG + chr4_4:59432731-59432750 Msa0691350:CDS 55.0%
AAGTGCACGAGACACTCGTC+AGG + chr4_4:59433651-59433670 Msa0691350:intron 55.0%
GTGCAAGCTGTGGAACAGCT+AGG + chr4_4:59433742-59433761 Msa0691350:CDS 55.0%
AGCTGTGGAACAGCTAGGAG+TGG + chr4_4:59433747-59433766 Msa0691350:CDS 55.0%
GGAACAGCTAGGAGTGGACA+AGG + chr4_4:59433753-59433772 Msa0691350:CDS 55.0%
ACAGCTAGGAGTGGACAAGG+CGG + chr4_4:59433756-59433775 Msa0691350:CDS 55.0%
! GGCGGTGCCTTCAAGGATTT+TGG + chr4_4:59433774-59433793 Msa0691350:CDS 55.0%
GCCACTCTCATGGACACCAT+CGG + chr4_4:59434302-59434321 Msa0691350:CDS 55.0%
ACCGATGGTGTCCATGAGAG+TGG - chr4_4:59434306-59434325 None:intergenic 55.0%
! GAAGGATCTTGAGGTGGTGG+AGG - chr4_4:59434333-59434352 None:intergenic 55.0%
GGTTAGAGAGAGCTACCTGC+TGG - chr4_4:59434374-59434393 None:intergenic 55.0%
CCACTGTTGTCAGAGGTGGT+GGG - chr4_4:59435819-59435838 None:intergenic 55.0%
ACCACTGTTGTCAGAGGTGG+TGG - chr4_4:59435820-59435839 None:intergenic 55.0%
GCTTTACACATGCCATGGGG+TGG - chr4_4:59436123-59436142 None:intergenic 55.0%
ATTAGCACCCTTCCTGCTCC+CGG + chr4_4:59436153-59436172 Msa0691350:CDS 55.0%
GGGAAGTCGAAGAAGTGACC+GGG - chr4_4:59436174-59436193 None:intergenic 55.0%
!! CCAAGTGGTAGTGGCAACCA+TGG - chr4_4:59436533-59436552 None:intergenic 55.0%
CCATGGTTGCCACTACCACT+TGG + chr4_4:59436530-59436549 Msa0691350:CDS 55.0%
!! GCTGGAGCTTTGAGTCCAAG+TGG - chr4_4:59436548-59436567 None:intergenic 55.0%
GGTGTTCTCCAATGACGCCG+AGG - chr4_4:59432772-59432791 None:intergenic 60.0%
TGGACAAGGCGGTGCCTTCA+AGG + chr4_4:59433767-59433786 Msa0691350:CDS 60.0%
!! AAACCCATGGTGGGTGCAGC+TGG - chr4_4:59434180-59434199 None:intergenic 60.0%
TGAGGGGGGAAACCCATGGT+GGG - chr4_4:59434189-59434208 None:intergenic 60.0%
ATGAGGGGGGAAACCCATGG+TGG - chr4_4:59434190-59434209 None:intergenic 60.0%
AGGATGAGGGGGGAAACCCA+TGG - chr4_4:59434193-59434212 None:intergenic 60.0%
TGAGGAGGAGGAGGATGAGG+GGG - chr4_4:59434204-59434223 None:intergenic 60.0%
GTGAGGAGGAGGAGGATGAG+GGG - chr4_4:59434205-59434224 None:intergenic 60.0%
GGTGAGGAGGAGGAGGATGA+GGG - chr4_4:59434206-59434225 None:intergenic 60.0%
TGGTGAGGAGGAGGAGGATG+AGG - chr4_4:59434207-59434226 None:intergenic 60.0%
CATGAGAGTGGCTGCGGTGT+AGG - chr4_4:59434294-59434313 None:intergenic 60.0%
CTACACCGCAGCCACTCTCA+TGG + chr4_4:59434292-59434311 Msa0691350:CDS 60.0%
GGTGTCCATGAGAGTGGCTG+CGG - chr4_4:59434300-59434319 None:intergenic 60.0%
CCCACCACCTCTGACAACAG+TGG + chr4_4:59435816-59435835 Msa0691350:CDS 60.0%
GAAGTGACCGGGAGCAGGAA+GGG - chr4_4:59436163-59436182 None:intergenic 60.0%
AGAAGTGACCGGGAGCAGGA+AGG - chr4_4:59436164-59436183 None:intergenic 60.0%
TCGAAGAAGTGACCGGGAGC+AGG - chr4_4:59436168-59436187 None:intergenic 60.0%
CCTAGCTAGCTCCCCGGTAA+AGG - chr4_4:59436656-59436675 None:intergenic 60.0%
CCTTTACCGGGGAGCTAGCT+AGG + chr4_4:59436653-59436672 Msa0691350:three_prime_UTR 60.0%
!! ATGGTGGGTGCAGCTGGCCA+TGG - chr4_4:59434174-59434193 None:intergenic 65.0%
ATGGCCAGCTGCACCCACCA+TGG + chr4_4:59434173-59434192 Msa0691350:CDS 65.0%
TGGCCAGCTGCACCCACCAT+GGG + chr4_4:59434174-59434193 Msa0691350:CDS 65.0%
GAGGAGGAGGAGGATGAGGG+GGG - chr4_4:59434203-59434222 None:intergenic 65.0%
!! GGTGGTGGAGGAGCTACCGA+TGG - chr4_4:59434321-59434340 None:intergenic 65.0%
ATGCCATGGGGTGGGATGCC+GGG - chr4_4:59436114-59436133 None:intergenic 65.0%
CATGCCATGGGGTGGGATGC+CGG - chr4_4:59436115-59436134 None:intergenic 65.0%
TGGGGTGGGATGCCGGGGAT+TGG - chr4_4:59436108-59436127 None:intergenic 70.0%
ATCCCCGGCATCCCACCCCA+TGG + chr4_4:59436108-59436127 Msa0691350:CDS 70.0%
TGCCATGGGGTGGGATGCCG+GGG - chr4_4:59436113-59436132 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 59432264 59436913 59432264 ID=Msa0691350;Name=Msa0691350
chr4_4 mRNA 59432264 59436913 59432264 ID=Msa0691350-mRNA-1;Parent=Msa0691350;Name=Msa0691350-mRNA-1;_AED=0.12;_eAED=0.12;_QI=246|1|1|1|1|1|6|276|419
chr4_4 exon 59432264 59432983 59432264 ID=Msa0691350-mRNA-1:exon:16148;Parent=Msa0691350-mRNA-1
chr4_4 exon 59433709 59433849 59433709 ID=Msa0691350-mRNA-1:exon:16149;Parent=Msa0691350-mRNA-1
chr4_4 exon 59434060 59434377 59434060 ID=Msa0691350-mRNA-1:exon:16150;Parent=Msa0691350-mRNA-1
chr4_4 exon 59435775 59435837 59435775 ID=Msa0691350-mRNA-1:exon:16151;Parent=Msa0691350-mRNA-1
chr4_4 exon 59436087 59436197 59436087 ID=Msa0691350-mRNA-1:exon:16152;Parent=Msa0691350-mRNA-1
chr4_4 exon 59436485 59436913 59436485 ID=Msa0691350-mRNA-1:exon:16153;Parent=Msa0691350-mRNA-1
chr4_4 five_prime_UTR 59432264 59432509 59432264 ID=Msa0691350-mRNA-1:five_prime_utr;Parent=Msa0691350-mRNA-1
chr4_4 CDS 59432510 59432983 59432510 ID=Msa0691350-mRNA-1:cds;Parent=Msa0691350-mRNA-1
chr4_4 CDS 59433709 59433849 59433709 ID=Msa0691350-mRNA-1:cds;Parent=Msa0691350-mRNA-1
chr4_4 CDS 59434060 59434377 59434060 ID=Msa0691350-mRNA-1:cds;Parent=Msa0691350-mRNA-1
chr4_4 CDS 59435775 59435837 59435775 ID=Msa0691350-mRNA-1:cds;Parent=Msa0691350-mRNA-1
chr4_4 CDS 59436087 59436197 59436087 ID=Msa0691350-mRNA-1:cds;Parent=Msa0691350-mRNA-1
chr4_4 CDS 59436485 59436637 59436485 ID=Msa0691350-mRNA-1:cds;Parent=Msa0691350-mRNA-1
chr4_4 three_prime_UTR 59436638 59436913 59436638 ID=Msa0691350-mRNA-1:three_prime_utr;Parent=Msa0691350-mRNA-1
Gene Sequence

>Msa0691350

ATGCTTGCAGTGTCACCATTGAGGAACACTAACAAAGATGGAAACCAAGAAGAGATGGAGAGTTTTTCGATGGGGAGTACTACAATTACAACGGGCGACTTCGGAGACCTTTCCGAAGGAAATTTATTGGAAAGCATCAACTTCGACGACTTCTTTGTTGGCATCAACGTCGATGGAGACATCTTACCGGATTTGGTAATGGATCCAGAGATGTTTGCTGAGTTCTCCGTTAGTTACGGCGAGGAATCAGAGATGAATTCCTCGGCGTCATTGGAGAACACCAGCAAAGTGGAAAAGAAGAATTCAAAGAAAGATTGTGAGGATAAAACAAGTTGTTCGGGTCAAGGCTCGGAAGAGATAGCGAGTAAAAGAGATGAATCTGTGGTTGTGAATCCATTGTTAAAAGATGCAGGTGGAAAAGGAAGAAAATCATCGAGTAAATCTAAGAATCCTCAAGGGAAGAGAAAAGTCAAGGTGGATTGGACACCAGACTTGCACAGGAGATTTGTGCAAGCTGTGGAACAGCTAGGAGTGGACAAGGCGGTGCCTTCAAGGATTTTGGAGATTATGGGAATTGATTGTCTCACTCGCCATAACATTGCTAGTCACCTTCAGAAATATAGGTCACATAGAAAACATTTGCTAGCGCGTGAAGCAGAAGCAGCAAGTTGGAATCAAAGGAGACAAGTATATGGTGGTGTAGGGGTGAAGAGAGAAGTGATCAGTCCATGGCCAGCTGCACCCACCATGGGTTTCCCCCCTCATCCTCCTCCTCCTCACCATTTTAGACCCTTACATGTATGGGGACATCCTTCAAATATGGACCAGTCTTTTCTGCACATGTGGCCTACACCGCAGCCACTCTCATGGACACCATCGGTAGCTCCTCCACCACCTCAAGATCCTTCATTTTGCCATTCTCATCACCAGCAGACTCCATTAAACGCAATGATCCGGCCAGGAACACATTGTTTCCCACCACCTCTGACAACAGTGAGACCATCATTTGGATGTCCAATCCCCGGCATCCCACCCCATGGCATGTGTAAAGCAGATCACGGCATTAGCACCCTTCCTGCTCCCGGTCACTTCTTCGACTTCCCACCGTCAAATGAGAGCATAGACGCAGCTATTGGAGATGTTTTATCAAAACCATGGTTGCCACTACCACTTGGACTCAAAGCTCCAGCACTTGATAGCGTAATGGGTGAACTACAAAGACAAGGAATTTCAAACATTCCACCCTCATCATGCACTTGA
Protein sequence

>Msa0691350

MLAVSPLRNTNKDGNQEEMESFSMGSTTITTGDFGDLSEGNLLESINFDDFFVGINVDGDILPDLVMDPEMFAEFSVSYGEESEMNSSASLENTSKVEKKNSKKDCEDKTSCSGQGSEEIASKRDESVVVNPLLKDAGGKGRKSSSKSKNPQGKRKVKVDWTPDLHRRFVQAVEQLGVDKAVPSRILEIMGIDCLTRHNIASHLQKYRSHRKHLLAREAEAASWNQRRQVYGGVGVKREVISPWPAAPTMGFPPHPPPPHHFRPLHVWGHPSNMDQSFLHMWPTPQPLSWTPSVAPPPPQDPSFCHSHHQQTPLNAMIRPGTHCFPPPLTTVRPSFGCPIPGIPPHGMCKADHGISTLPAPGHFFDFPPSNESIDAAIGDVLSKPWLPLPLGLKAPALDSVMGELQRQGISNIPPSSCT*