Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0844330 | XP_003613544.1 | 97.619 | 126 | 3 | 0 | 1 | 126 | 1 | 126 | 5.97e-88 | 261 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0844330 | sp|Q38879|TRXH2_ARATH | 48.649 | 111 | 57 | 0 | 15 | 125 | 22 | 132 | 5.98e-41 | 135 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0844330 | D8L5E0 | 97.619 | 126 | 3 | 0 | 1 | 126 | 1 | 126 | 2.85e-88 | 261 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0035090 | Msa0844330 | 0.806862 | 6.694253e-50 | -8.615850e-47 |
| Msa0039200 | Msa0844330 | 0.820645 | 6.146524e-53 | -8.615850e-47 |
| Msa0074310 | Msa0844330 | 0.809086 | 2.251592e-50 | -8.615850e-47 |
| Msa0083200 | Msa0844330 | 0.823468 | 1.362595e-53 | -8.615850e-47 |
| Msa0086820 | Msa0844330 | 0.819100 | 1.386743e-52 | -8.615850e-47 |
| Msa0112800 | Msa0844330 | 0.815468 | 9.102333e-52 | -8.615850e-47 |
| Msa0624890 | Msa0844330 | 0.815771 | 7.792190e-52 | -8.615850e-47 |
| Msa0626890 | Msa0844330 | 0.803024 | 4.246465e-49 | -8.615850e-47 |
| Msa0681040 | Msa0844330 | 0.803419 | 3.517577e-49 | -8.615850e-47 |
| Msa0713530 | Msa0844330 | 0.818605 | 1.796047e-52 | -8.615850e-47 |
| Msa0724490 | Msa0844330 | 0.927220 | 1.591528e-91 | -8.615850e-47 |
| Msa0724500 | Msa0844330 | 0.902216 | 1.215652e-78 | -8.615850e-47 |
| Msa0736930 | Msa0844330 | 0.804437 | 2.161302e-49 | -8.615850e-47 |
| Msa0751000 | Msa0844330 | 0.821819 | 3.296826e-53 | -8.615850e-47 |
| Msa0755590 | Msa0844330 | 0.832490 | 9.177630e-56 | -8.615850e-47 |
| Msa0765010 | Msa0844330 | 0.849287 | 3.563068e-60 | -8.615850e-47 |
| Msa0765020 | Msa0844330 | 0.928926 | 1.444279e-92 | -8.615850e-47 |
| Msa0765040 | Msa0844330 | 0.877927 | 4.243027e-69 | -8.615850e-47 |
| Msa0766880 | Msa0844330 | 0.800965 | 1.124898e-48 | -8.615850e-47 |
| Msa0794040 | Msa0844330 | 0.839043 | 2.005882e-57 | -8.615850e-47 |
| Msa0797240 | Msa0844330 | 0.806393 | 8.408639e-50 | -8.615850e-47 |
| Msa0818950 | Msa0844330 | 0.806357 | 8.556622e-50 | -8.615850e-47 |
| Msa0835010 | Msa0844330 | 0.821843 | 3.253975e-53 | -8.615850e-47 |
| Msa0130120 | Msa0844330 | 0.808609 | 2.847867e-50 | -8.615850e-47 |
| Msa0133670 | Msa0844330 | 0.818865 | 1.568298e-52 | -8.615850e-47 |
| Msa0133880 | Msa0844330 | 0.825603 | 4.283544e-54 | -8.615850e-47 |
| Msa0156780 | Msa0844330 | 0.803780 | 2.959905e-49 | -8.615850e-47 |
| Msa0163500 | Msa0844330 | 0.801022 | 1.095113e-48 | -8.615850e-47 |
| Msa0166580 | Msa0844330 | 0.813694 | 2.248078e-51 | -8.615850e-47 |
| Msa0175480 | Msa0844330 | 0.838236 | 3.242874e-57 | -8.615850e-47 |
| Msa0184120 | Msa0844330 | 0.819402 | 1.183661e-52 | -8.615850e-47 |
| Msa0187720 | Msa0844330 | 0.811052 | 8.490185e-51 | -8.615850e-47 |
| Msa0207530 | Msa0844330 | 0.820573 | 6.387629e-53 | -8.615850e-47 |
| Msa0207900 | Msa0844330 | 0.817911 | 2.579608e-52 | -8.615850e-47 |
| Msa0216010 | Msa0844330 | 0.807418 | 5.105330e-50 | -8.615850e-47 |
| Msa0216560 | Msa0844330 | 0.836087 | 1.149033e-56 | -8.615850e-47 |
| Msa0369750 | Msa0844330 | 0.818696 | 1.713234e-52 | -8.615850e-47 |
| Msa0377460 | Msa0844330 | 0.805027 | 1.627685e-49 | -8.615850e-47 |
| Msa0426890 | Msa0844330 | 0.804091 | 2.550655e-49 | -8.615850e-47 |
| Msa0429130 | Msa0844330 | 0.802398 | 5.716343e-49 | -8.615850e-47 |
| Msa0463960 | Msa0844330 | 0.805123 | 1.554042e-49 | -8.615850e-47 |
| Msa0256170 | Msa0844330 | 0.827388 | 1.608637e-54 | -8.615850e-47 |
| Msa0295840 | Msa0844330 | 0.831855 | 1.317519e-55 | -8.615850e-47 |
| Msa0509510 | Msa0844330 | 0.809691 | 1.669814e-50 | -8.615850e-47 |
| Msa0517400 | Msa0844330 | 0.800777 | 1.229299e-48 | -8.615850e-47 |
| Msa0540120 | Msa0844330 | 0.804933 | 1.702441e-49 | -8.615850e-47 |
| Msa0587060 | Msa0844330 | 0.804727 | 1.880419e-49 | -8.615850e-47 |
| Msa0601710 | Msa0844330 | 0.803974 | 2.698640e-49 | -8.615850e-47 |
| Msa0844330 | Msa0844350 | 0.927588 | 9.527105e-92 | -8.615850e-47 |
| Msa0844330 | Msa0967450 | 0.809692 | 1.669077e-50 | -8.615850e-47 |
| Msa0844330 | Msa1031220 | 0.815718 | 8.006299e-52 | -8.615850e-47 |
| Msa0844330 | Msa1041550 | 0.814350 | 1.610974e-51 | -8.615850e-47 |
| Msa0844330 | Msa1070810 | -0.801292 | 9.647559e-49 | -8.615850e-47 |
| Msa0844330 | Msa1084630 | 0.803882 | 2.819700e-49 | -8.615850e-47 |
| Msa0844330 | Msa1124180 | 0.811552 | 6.611956e-51 | -8.615850e-47 |
| Msa0844330 | Msa1132320 | 0.825011 | 5.914249e-54 | -8.615850e-47 |
| Msa0844330 | Msa1158630 | -0.802018 | 6.844928e-49 | -8.615850e-47 |
| Msa0844330 | Msa1166450 | 0.825551 | 4.408523e-54 | -8.615850e-47 |
| Msa0844330 | Msa1167820 | 0.800125 | 1.668850e-48 | -8.615850e-47 |
| Msa0844330 | Msa1173410 | 0.819085 | 1.397694e-52 | -8.615850e-47 |
| Msa0844330 | Msa1175590 | 0.803778 | 2.963457e-49 | -8.615850e-47 |
| Msa0844330 | Msa1188380 | 0.847698 | 9.810474e-60 | -8.615850e-47 |
| Msa0844330 | Msa1195860 | 0.800614 | 1.327113e-48 | -8.615850e-47 |
| Msa0844330 | Msa1199690 | 0.802211 | 6.248449e-49 | -8.615850e-47 |
| Msa0844330 | Msa1224690 | 0.843575 | 1.287854e-58 | -8.615850e-47 |
| Msa0844330 | Msa1235770 | 0.820560 | 6.431025e-53 | -8.615850e-47 |
| Msa0844330 | Msa1242430 | 0.825629 | 4.224167e-54 | -8.615850e-47 |
| Msa0844330 | Msa1242990 | 0.819840 | 9.400599e-53 | -8.615850e-47 |
| Msa0844330 | Msa1254470 | 0.802746 | 4.845951e-49 | -8.615850e-47 |
| Msa0844330 | Msa1264230 | 0.816058 | 6.724015e-52 | -8.615850e-47 |
| Msa0844330 | Msa1265250 | 0.811019 | 8.630386e-51 | -8.615850e-47 |
| Msa0844330 | Msa1266720 | 0.810519 | 1.106938e-50 | -8.615850e-47 |
| Msa0844330 | Msa1294520 | 0.836709 | 7.982329e-57 | -8.615850e-47 |
| Msa0844330 | Msa1302920 | 0.830217 | 3.325116e-55 | -8.615850e-47 |
| Msa0844330 | Msa1309910 | 0.811939 | 5.446217e-51 | -8.615850e-47 |
| Msa0844330 | Msa1351560 | 0.831962 | 1.239544e-55 | -8.615850e-47 |
| Msa0844330 | Msa1357720 | 0.800107 | 1.682710e-48 | -8.615850e-47 |
| Msa0844330 | Msa1377170 | 0.810116 | 1.352448e-50 | -8.615850e-47 |
| Msa0844330 | Msa1385940 | 0.820434 | 6.873229e-53 | -8.615850e-47 |
| Msa0844330 | Msa1400330 | 0.803861 | 2.847921e-49 | -8.615850e-47 |
| Msa0844330 | Msa1400600 | 0.810101 | 1.362957e-50 | -8.615850e-47 |
| Msa0844330 | Msa1407200 | 0.833780 | 4.382213e-56 | -8.615850e-47 |
| Msa0844330 | Msa1409050 | 0.844689 | 6.469753e-59 | -8.615850e-47 |
| Msa0844330 | Msa1409760 | 0.838812 | 2.301970e-57 | -8.615850e-47 |
| Msa0844330 | Msa1414830 | 0.817309 | 3.525170e-52 | -8.615850e-47 |
| Msa0844330 | Msa1415120 | 0.800800 | 1.215901e-48 | -8.615850e-47 |
| Msa0844330 | Msa1433380 | 0.804130 | 2.504041e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0844330 | MtrunA17_Chr5g0415741 | 97.619 | 126 | 3 | 0 | 1 | 126 | 1 | 126 | 5.49e-92 | 261 |
| Msa0844330 | MtrunA17_Chr5g0415771 | 66.667 | 126 | 41 | 1 | 1 | 125 | 1 | 126 | 2.05e-52 | 161 |
| Msa0844330 | MtrunA17_Chr5g0415761 | 66.406 | 128 | 41 | 1 | 1 | 126 | 1 | 128 | 2.84e-52 | 161 |
| Msa0844330 | MtrunA17_Chr7g0216481 | 57.547 | 106 | 45 | 0 | 20 | 125 | 28 | 133 | 2.63e-44 | 141 |
| Msa0844330 | MtrunA17_Chr3g0142051 | 47.748 | 111 | 58 | 0 | 14 | 124 | 2 | 112 | 1.62e-36 | 120 |
| Msa0844330 | MtrunA17_Chr1g0155721 | 43.363 | 113 | 64 | 0 | 14 | 126 | 2 | 114 | 9.31e-35 | 116 |
| Msa0844330 | MtrunA17_Chr5g0404831 | 45.045 | 111 | 61 | 0 | 14 | 124 | 4 | 114 | 1.44e-34 | 116 |
| Msa0844330 | MtrunA17_Chr8g0393201 | 42.308 | 104 | 60 | 0 | 18 | 121 | 227 | 330 | 6.33e-25 | 96.7 |
| Msa0844330 | MtrunA17_Chr8g0334481 | 34.286 | 105 | 69 | 0 | 17 | 121 | 25 | 129 | 1.93e-23 | 88.2 |
| Msa0844330 | MtrunA17_Chr7g0245141 | 36.190 | 105 | 67 | 0 | 17 | 121 | 23 | 127 | 2.56e-23 | 88.2 |
| Msa0844330 | MtrunA17_Chr2g0280411 | 39.048 | 105 | 64 | 0 | 18 | 122 | 9 | 113 | 4.34e-23 | 87.0 |
| Msa0844330 | MtrunA17_Chr4g0042051 | 37.383 | 107 | 63 | 2 | 18 | 122 | 10 | 114 | 3.90e-21 | 82.0 |
| Msa0844330 | MtrunA17_Chr2g0319291 | 32.381 | 105 | 71 | 0 | 18 | 122 | 10 | 114 | 6.62e-20 | 79.0 |
| Msa0844330 | MtrunA17_Chr1g0201781 | 33.721 | 86 | 54 | 2 | 39 | 123 | 77 | 160 | 1.34e-15 | 68.9 |
| Msa0844330 | MtrunA17_Chr7g0249461 | 37.500 | 88 | 54 | 1 | 40 | 126 | 98 | 185 | 4.79e-15 | 67.8 |
| Msa0844330 | MtrunA17_Chr4g0052481 | 34.146 | 82 | 52 | 1 | 44 | 123 | 85 | 166 | 2.75e-14 | 65.5 |
| Msa0844330 | MtrunA17_Chr8g0360031 | 34.091 | 88 | 56 | 2 | 38 | 123 | 87 | 174 | 4.10e-14 | 65.1 |
| Msa0844330 | MtrunA17_Chr3g0125821 | 31.313 | 99 | 63 | 3 | 27 | 123 | 76 | 171 | 1.09e-13 | 63.9 |
| Msa0844330 | MtrunA17_Chr2g0317731 | 34.091 | 88 | 56 | 2 | 38 | 123 | 84 | 171 | 3.04e-13 | 62.8 |
| Msa0844330 | MtrunA17_Chr1g0211871 | 31.646 | 79 | 50 | 2 | 38 | 115 | 84 | 159 | 5.67e-12 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0844330 | AT5G39950.1 | 48.649 | 111 | 57 | 0 | 15 | 125 | 22 | 132 | 6.08e-42 | 135 |
| Msa0844330 | AT1G69880.1 | 47.664 | 107 | 56 | 0 | 17 | 123 | 36 | 142 | 5.77e-35 | 118 |
| Msa0844330 | AT1G59730.1 | 41.406 | 128 | 72 | 1 | 1 | 125 | 1 | 128 | 6.26e-35 | 117 |
| Msa0844330 | AT3G51030.1 | 42.727 | 110 | 63 | 0 | 14 | 123 | 2 | 111 | 2.86e-33 | 112 |
| Msa0844330 | AT1G19730.1 | 46.429 | 112 | 59 | 1 | 14 | 124 | 2 | 113 | 1.38e-32 | 111 |
| Msa0844330 | AT1G45145.1 | 43.925 | 107 | 60 | 0 | 18 | 124 | 5 | 111 | 2.17e-31 | 108 |
| Msa0844330 | AT5G42980.1 | 44.037 | 109 | 61 | 0 | 18 | 126 | 5 | 113 | 7.94e-31 | 107 |
| Msa0844330 | AT3G17880.2 | 40.777 | 103 | 61 | 0 | 19 | 121 | 264 | 366 | 1.45e-22 | 91.3 |
| Msa0844330 | AT3G17880.1 | 40.777 | 103 | 61 | 0 | 19 | 121 | 271 | 373 | 1.46e-22 | 91.3 |
| Msa0844330 | AT3G56420.1 | 38.043 | 92 | 57 | 0 | 28 | 119 | 50 | 141 | 2.87e-21 | 83.6 |
| Msa0844330 | AT3G56420.2 | 38.043 | 92 | 57 | 0 | 28 | 119 | 50 | 141 | 2.87e-21 | 83.6 |
| Msa0844330 | AT3G56420.3 | 38.043 | 92 | 57 | 0 | 28 | 119 | 50 | 141 | 2.87e-21 | 83.6 |
| Msa0844330 | AT3G08710.2 | 33.333 | 111 | 72 | 1 | 11 | 121 | 18 | 126 | 1.50e-20 | 81.3 |
| Msa0844330 | AT3G08710.3 | 33.333 | 111 | 72 | 1 | 11 | 121 | 18 | 126 | 1.50e-20 | 81.3 |
| Msa0844330 | AT3G08710.1 | 33.333 | 111 | 72 | 1 | 11 | 121 | 18 | 126 | 1.50e-20 | 81.3 |
| Msa0844330 | AT1G11530.1 | 34.286 | 105 | 69 | 0 | 18 | 122 | 2 | 106 | 5.31e-19 | 76.6 |
| Msa0844330 | AT2G40790.2 | 34.783 | 92 | 60 | 0 | 28 | 119 | 50 | 141 | 3.95e-17 | 72.8 |
| Msa0844330 | AT2G40790.1 | 34.783 | 92 | 60 | 0 | 28 | 119 | 50 | 141 | 3.95e-17 | 72.8 |
| Msa0844330 | AT5G16400.1 | 38.636 | 88 | 53 | 1 | 40 | 126 | 97 | 184 | 2.95e-16 | 71.2 |
| Msa0844330 | AT1G43560.1 | 29.915 | 117 | 77 | 3 | 1 | 112 | 33 | 149 | 3.01e-16 | 70.9 |
| Msa0844330 | AT3G02730.1 | 40.230 | 87 | 51 | 1 | 41 | 126 | 88 | 174 | 5.61e-16 | 70.5 |
| Msa0844330 | AT4G03520.1 | 40.845 | 71 | 41 | 1 | 43 | 112 | 101 | 171 | 4.03e-15 | 68.6 |
| Msa0844330 | AT1G76760.1 | 30.526 | 95 | 59 | 3 | 33 | 120 | 70 | 164 | 1.45e-14 | 66.6 |
| Msa0844330 | AT1G03680.1 | 38.272 | 81 | 48 | 2 | 45 | 123 | 97 | 177 | 1.79e-14 | 66.6 |
| Msa0844330 | AT3G15360.1 | 32.967 | 91 | 53 | 3 | 38 | 123 | 102 | 189 | 2.73e-14 | 66.2 |
| Msa0844330 | AT2G35010.2 | 37.349 | 83 | 48 | 2 | 44 | 123 | 110 | 191 | 2.41e-13 | 63.9 |
| Msa0844330 | AT2G35010.1 | 37.349 | 83 | 48 | 2 | 44 | 123 | 110 | 191 | 2.41e-13 | 63.9 |
| Msa0844330 | AT1G31020.1 | 30.000 | 110 | 75 | 1 | 16 | 123 | 47 | 156 | 2.58e-13 | 63.2 |
| Msa0844330 | AT1G52990.1 | 34.066 | 91 | 58 | 2 | 34 | 123 | 222 | 311 | 2.95e-12 | 62.4 |
| Msa0844330 | AT1G50320.1 | 35.443 | 79 | 47 | 2 | 38 | 115 | 85 | 160 | 4.97e-12 | 60.1 |
Find 39 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTCCATTCCACTGCTAAA+TGG | 0.238150 | 5_4:-22752487 | Msa0844330:CDS |
| TCAATAAAATAATATATAAT+TGG | 0.258995 | 5_4:-22751741 | Msa0844330:three_prime_UTR |
| GCTTGATGTTGATGAGTTAT+TGG | 0.264813 | 5_4:-22752238 | Msa0844330:intron |
| GCAGCAAAATCTTTCAAAAC+TGG | 0.287096 | 5_4:+22752288 | None:intergenic |
| CTCATTCTCCTTTGATTTCA+AGG | 0.289089 | 5_4:-22751902 | Msa0844330:three_prime_UTR |
| TTATTGATTTCACAGCTAAA+TGG | 0.322109 | 5_4:-22752335 | Msa0844330:CDS |
| TGTTAATTTGTTGGGATGAT+TGG | 0.356739 | 5_4:+22752653 | None:intergenic |
| TTGTTGGGATGATTGGAAAT+TGG | 0.378161 | 5_4:+22752660 | None:intergenic |
| GGGAGTGTCTCAAACCTTCC+AGG | 0.392325 | 5_4:-22752076 | Msa0844330:CDS |
| TATGATGTTTGTTAATTTGT+TGG | 0.397122 | 5_4:+22752644 | None:intergenic |
| TAAAGTGGTGGGAGCAAGAA+AGG | 0.432390 | 5_4:-22752004 | Msa0844330:CDS |
| TTCTTAATGAGTAGAAAAGT+TGG | 0.438267 | 5_4:+22752042 | None:intergenic |
| TTCAAGGGATCTACAAGATC+AGG | 0.446787 | 5_4:-22751886 | Msa0844330:three_prime_UTR |
| ATGATGTTTGTTAATTTGTT+GGG | 0.449780 | 5_4:+22752645 | None:intergenic |
| GGGATGATTGGAAATTGGTT+GGG | 0.476532 | 5_4:+22752665 | None:intergenic |
| CACTCAAAGGATACAAATAT+AGG | 0.481261 | 5_4:-22751837 | Msa0844330:three_prime_UTR |
| TCATTCTCCTTTGATTTCAA+GGG | 0.482125 | 5_4:-22751901 | Msa0844330:three_prime_UTR |
| TGGGATGATTGGAAATTGGT+TGG | 0.482987 | 5_4:+22752664 | None:intergenic |
| GTGTGGACCTTGCAAAGTTA+TGG | 0.487071 | 5_4:-22752313 | Msa0844330:CDS |
| AGGAAAGGTTGTTGATAAAG+TGG | 0.493130 | 5_4:-22752019 | Msa0844330:CDS |
| TAGCAGTGGAATGGAATGTA+AGG | 0.498154 | 5_4:+22752492 | None:intergenic |
| TCTACTCATTAAGAAAGGAA+AGG | 0.517021 | 5_4:-22752034 | Msa0844330:CDS |
| ATACACAAAATTATACAAGT+TGG | 0.527315 | 5_4:+22752544 | None:intergenic |
| CATTCCATTTAGCAGTGGAA+TGG | 0.530623 | 5_4:+22752483 | None:intergenic |
| GGGATATATATAGTGTAGAA+AGG | 0.532837 | 5_4:+22752685 | None:intergenic |
| GTGTCATCAATTTCACTCAA+AGG | 0.533950 | 5_4:-22751850 | Msa0844330:three_prime_UTR |
| AAGATCAGGATGCAATAAGT+TGG | 0.563550 | 5_4:-22751872 | Msa0844330:three_prime_UTR |
| TGGAATGGAATGTAAGGACA+TGG | 0.568326 | 5_4:+22752498 | None:intergenic |
| AAGTTGGCAATGAATGCACC+TGG | 0.579245 | 5_4:+22752058 | None:intergenic |
| TGTAGATCCCTTGAAATCAA+AGG | 0.581835 | 5_4:+22751894 | None:intergenic |
| TGGCAATGAATGCACCTGGA+AGG | 0.589602 | 5_4:+22752062 | None:intergenic |
| GATTTCACAGCTAAATGGTG+TGG | 0.590907 | 5_4:-22752330 | Msa0844330:CDS |
| AAGGTTGTTGATAAAGTGGT+GGG | 0.601867 | 5_4:-22752015 | Msa0844330:CDS |
| ACTGGATCCATAACTTTGCA+AGG | 0.608925 | 5_4:+22752306 | None:intergenic |
| ATGAGCATTCCATTTAGCAG+TGG | 0.609708 | 5_4:+22752478 | None:intergenic |
| AAAGGTTGTTGATAAAGTGG+TGG | 0.610487 | 5_4:-22752016 | Msa0844330:CDS |
| CAAGAAAGGAGGAACTACAA+AGG | 0.696570 | 5_4:-22751990 | Msa0844330:CDS |
| AGTGGTGGGAGCAAGAAAGG+AGG | 0.703427 | 5_4:-22752001 | Msa0844330:CDS |
| GGAATGGAATGTAAGGACAT+GGG | 0.736550 | 5_4:+22752499 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTAAAGAAACTAATAAAC+TGG | - | chr5_4:22751942-22751961 | Msa0844330:three_prime_UTR | 15.0% |
| !!! | TTTTAAAGAAACTAATAAAC+TGG | - | chr5_4:22751942-22751961 | Msa0844330:three_prime_UTR | 15.0% |
| !!! | ATGATGTTTGTTAATTTGTT+GGG | + | chr5_4:22751747-22751766 | None:intergenic | 20.0% |
| !!! | TATGATGTTTGTTAATTTGT+TGG | + | chr5_4:22751748-22751767 | None:intergenic | 20.0% |
| !!! | CATACACCTAAATAATTTTT+TGG | - | chr5_4:22751794-22751813 | Msa0844330:three_prime_UTR | 20.0% |
| !! | GATGAACCAAAAAATTATTT+AGG | + | chr5_4:22751803-22751822 | None:intergenic | 20.0% |
| !! | ATACACAAAATTATACAAGT+TGG | + | chr5_4:22751848-22751867 | None:intergenic | 20.0% |
| !!! | TCTTATGCATTATTTTTCTT+TGG | - | chr5_4:22752022-22752041 | Msa0844330:CDS | 20.0% |
| !!! | TCATTCTTTTATCATATAAG+AGG | - | chr5_4:22752577-22752596 | Msa0844330:five_prime_UTR | 20.0% |
| !!! | ATGATGTTTGTTAATTTGTT+GGG | + | chr5_4:22751747-22751766 | None:intergenic | 20.0% |
| !!! | TATGATGTTTGTTAATTTGT+TGG | + | chr5_4:22751748-22751767 | None:intergenic | 20.0% |
| !!! | CATACACCTAAATAATTTTT+TGG | - | chr5_4:22751794-22751813 | Msa0844330:three_prime_UTR | 20.0% |
| !! | GATGAACCAAAAAATTATTT+AGG | + | chr5_4:22751803-22751822 | None:intergenic | 20.0% |
| !! | ATACACAAAATTATACAAGT+TGG | + | chr5_4:22751848-22751867 | None:intergenic | 20.0% |
| !!! | TCTTATGCATTATTTTTCTT+TGG | - | chr5_4:22752022-22752041 | Msa0844330:CDS | 20.0% |
| !!! | TCATTCTTTTATCATATAAG+AGG | - | chr5_4:22752577-22752596 | Msa0844330:five_prime_UTR | 20.0% |
| !!! | TTATTTTTCTTTGGCATAGA+TGG | - | chr5_4:22752031-22752050 | Msa0844330:CDS | 25.0% |
| !!! | TTATTGATTTCACAGCTAAA+TGG | - | chr5_4:22752054-22752073 | Msa0844330:CDS | 25.0% |
| ! | TAACAAACTAGTTAAAGTGA+AGG | + | chr5_4:22752192-22752211 | None:intergenic | 25.0% |
| !! | TGTTTTTCCCACAATATAAT+TGG | - | chr5_4:22752285-22752304 | Msa0844330:CDS | 25.0% |
| ! | TTCTTAATGAGTAGAAAAGT+TGG | + | chr5_4:22752350-22752369 | None:intergenic | 25.0% |
| !! | ACTTTTCTACTCATTAAGAA+AGG | - | chr5_4:22752350-22752369 | Msa0844330:intron | 25.0% |
| !!! | TTATTTTTCTTTGGCATAGA+TGG | - | chr5_4:22752031-22752050 | Msa0844330:CDS | 25.0% |
| !!! | TTATTGATTTCACAGCTAAA+TGG | - | chr5_4:22752054-22752073 | Msa0844330:CDS | 25.0% |
| ! | TAACAAACTAGTTAAAGTGA+AGG | + | chr5_4:22752192-22752211 | None:intergenic | 25.0% |
| !! | TGTTTTTCCCACAATATAAT+TGG | - | chr5_4:22752285-22752304 | Msa0844330:CDS | 25.0% |
| ! | TTCTTAATGAGTAGAAAAGT+TGG | + | chr5_4:22752350-22752369 | None:intergenic | 25.0% |
| !! | ACTTTTCTACTCATTAAGAA+AGG | - | chr5_4:22752350-22752369 | Msa0844330:intron | 25.0% |
| GGGATATATATAGTGTAGAA+AGG | + | chr5_4:22751707-22751726 | None:intergenic | 30.0% | |
| !! | TGTTAATTTGTTGGGATGAT+TGG | + | chr5_4:22751739-22751758 | None:intergenic | 30.0% |
| TCTACTCATTAAGAAAGGAA+AGG | - | chr5_4:22752355-22752374 | Msa0844330:intron | 30.0% | |
| ! | TCATTCTCCTTTGATTTCAA+GGG | - | chr5_4:22752488-22752507 | Msa0844330:CDS | 30.0% |
| CACTCAAAGGATACAAATAT+AGG | - | chr5_4:22752552-22752571 | Msa0844330:CDS | 30.0% | |
| GGGATATATATAGTGTAGAA+AGG | + | chr5_4:22751707-22751726 | None:intergenic | 30.0% | |
| !! | TGTTAATTTGTTGGGATGAT+TGG | + | chr5_4:22751739-22751758 | None:intergenic | 30.0% |
| TCTACTCATTAAGAAAGGAA+AGG | - | chr5_4:22752355-22752374 | Msa0844330:intron | 30.0% | |
| ! | TCATTCTCCTTTGATTTCAA+GGG | - | chr5_4:22752488-22752507 | Msa0844330:CDS | 30.0% |
| CACTCAAAGGATACAAATAT+AGG | - | chr5_4:22752552-22752571 | Msa0844330:CDS | 30.0% | |
| TTGTTGGGATGATTGGAAAT+TGG | + | chr5_4:22751732-22751751 | None:intergenic | 35.0% | |
| GCAGCAAAATCTTTCAAAAC+TGG | + | chr5_4:22752104-22752123 | None:intergenic | 35.0% | |
| ! | GCTTGATGTTGATGAGTTAT+TGG | - | chr5_4:22752151-22752170 | Msa0844330:intron | 35.0% |
| CCTGTTGCCAATTATATTGT+GGG | + | chr5_4:22752295-22752314 | None:intergenic | 35.0% | |
| CCACAATATAATTGGCAACA+GGG | - | chr5_4:22752293-22752312 | Msa0844330:CDS | 35.0% | |
| ! | AGGAAAGGTTGTTGATAAAG+TGG | - | chr5_4:22752370-22752389 | Msa0844330:intron | 35.0% |
| ! | AAAGGTTGTTGATAAAGTGG+TGG | - | chr5_4:22752373-22752392 | Msa0844330:intron | 35.0% |
| ! | AAGGTTGTTGATAAAGTGGT+GGG | - | chr5_4:22752374-22752393 | Msa0844330:intron | 35.0% |
| ! | CTCATTCTCCTTTGATTTCA+AGG | - | chr5_4:22752487-22752506 | Msa0844330:CDS | 35.0% |
| TGTAGATCCCTTGAAATCAA+AGG | + | chr5_4:22752498-22752517 | None:intergenic | 35.0% | |
| AAGATCAGGATGCAATAAGT+TGG | - | chr5_4:22752517-22752536 | Msa0844330:CDS | 35.0% | |
| GTGTCATCAATTTCACTCAA+AGG | - | chr5_4:22752539-22752558 | Msa0844330:CDS | 35.0% | |
| TTGTTGGGATGATTGGAAAT+TGG | + | chr5_4:22751732-22751751 | None:intergenic | 35.0% | |
| GCAGCAAAATCTTTCAAAAC+TGG | + | chr5_4:22752104-22752123 | None:intergenic | 35.0% | |
| ! | GCTTGATGTTGATGAGTTAT+TGG | - | chr5_4:22752151-22752170 | Msa0844330:intron | 35.0% |
| CCTGTTGCCAATTATATTGT+GGG | + | chr5_4:22752295-22752314 | None:intergenic | 35.0% | |
| CCACAATATAATTGGCAACA+GGG | - | chr5_4:22752293-22752312 | Msa0844330:CDS | 35.0% | |
| ! | AGGAAAGGTTGTTGATAAAG+TGG | - | chr5_4:22752370-22752389 | Msa0844330:intron | 35.0% |
| ! | AAAGGTTGTTGATAAAGTGG+TGG | - | chr5_4:22752373-22752392 | Msa0844330:intron | 35.0% |
| ! | AAGGTTGTTGATAAAGTGGT+GGG | - | chr5_4:22752374-22752393 | Msa0844330:intron | 35.0% |
| ! | CTCATTCTCCTTTGATTTCA+AGG | - | chr5_4:22752487-22752506 | Msa0844330:CDS | 35.0% |
| TGTAGATCCCTTGAAATCAA+AGG | + | chr5_4:22752498-22752517 | None:intergenic | 35.0% | |
| AAGATCAGGATGCAATAAGT+TGG | - | chr5_4:22752517-22752536 | Msa0844330:CDS | 35.0% | |
| GTGTCATCAATTTCACTCAA+AGG | - | chr5_4:22752539-22752558 | Msa0844330:CDS | 35.0% | |
| GGGATGATTGGAAATTGGTT+GGG | + | chr5_4:22751727-22751746 | None:intergenic | 40.0% | |
| TGGGATGATTGGAAATTGGT+TGG | + | chr5_4:22751728-22751747 | None:intergenic | 40.0% | |
| GGAATGGAATGTAAGGACAT+GGG | + | chr5_4:22751893-22751912 | None:intergenic | 40.0% | |
| TGGAATGGAATGTAAGGACA+TGG | + | chr5_4:22751894-22751913 | None:intergenic | 40.0% | |
| TAGCAGTGGAATGGAATGTA+AGG | + | chr5_4:22751900-22751919 | None:intergenic | 40.0% | |
| CATTCCATTCCACTGCTAAA+TGG | - | chr5_4:22751902-22751921 | Msa0844330:three_prime_UTR | 40.0% | |
| CATTCCATTTAGCAGTGGAA+TGG | + | chr5_4:22751909-22751928 | None:intergenic | 40.0% | |
| ATGAGCATTCCATTTAGCAG+TGG | + | chr5_4:22751914-22751933 | None:intergenic | 40.0% | |
| GATTTCACAGCTAAATGGTG+TGG | - | chr5_4:22752059-22752078 | Msa0844330:CDS | 40.0% | |
| ACTGGATCCATAACTTTGCA+AGG | + | chr5_4:22752086-22752105 | None:intergenic | 40.0% | |
| ACAAATCAGTTCTCAAGTGC+TGG | + | chr5_4:22752268-22752287 | None:intergenic | 40.0% | |
| CCCACAATATAATTGGCAAC+AGG | - | chr5_4:22752292-22752311 | Msa0844330:CDS | 40.0% | |
| CCCTGTTGCCAATTATATTG+TGG | + | chr5_4:22752296-22752315 | None:intergenic | 40.0% | |
| CAAGAAAGGAGGAACTACAA+AGG | - | chr5_4:22752399-22752418 | Msa0844330:intron | 40.0% | |
| TTCAAGGGATCTACAAGATC+AGG | - | chr5_4:22752503-22752522 | Msa0844330:CDS | 40.0% | |
| GGGATGATTGGAAATTGGTT+GGG | + | chr5_4:22751727-22751746 | None:intergenic | 40.0% | |
| TGGGATGATTGGAAATTGGT+TGG | + | chr5_4:22751728-22751747 | None:intergenic | 40.0% | |
| GGAATGGAATGTAAGGACAT+GGG | + | chr5_4:22751893-22751912 | None:intergenic | 40.0% | |
| TGGAATGGAATGTAAGGACA+TGG | + | chr5_4:22751894-22751913 | None:intergenic | 40.0% | |
| TAGCAGTGGAATGGAATGTA+AGG | + | chr5_4:22751900-22751919 | None:intergenic | 40.0% | |
| CATTCCATTCCACTGCTAAA+TGG | - | chr5_4:22751902-22751921 | Msa0844330:three_prime_UTR | 40.0% | |
| CATTCCATTTAGCAGTGGAA+TGG | + | chr5_4:22751909-22751928 | None:intergenic | 40.0% | |
| ATGAGCATTCCATTTAGCAG+TGG | + | chr5_4:22751914-22751933 | None:intergenic | 40.0% | |
| GATTTCACAGCTAAATGGTG+TGG | - | chr5_4:22752059-22752078 | Msa0844330:CDS | 40.0% | |
| ACTGGATCCATAACTTTGCA+AGG | + | chr5_4:22752086-22752105 | None:intergenic | 40.0% | |
| ACAAATCAGTTCTCAAGTGC+TGG | + | chr5_4:22752268-22752287 | None:intergenic | 40.0% | |
| CCCACAATATAATTGGCAAC+AGG | - | chr5_4:22752292-22752311 | Msa0844330:CDS | 40.0% | |
| CCCTGTTGCCAATTATATTG+TGG | + | chr5_4:22752296-22752315 | None:intergenic | 40.0% | |
| CAAGAAAGGAGGAACTACAA+AGG | - | chr5_4:22752399-22752418 | Msa0844330:intron | 40.0% | |
| TTCAAGGGATCTACAAGATC+AGG | - | chr5_4:22752503-22752522 | Msa0844330:CDS | 40.0% | |
| GTGTGGACCTTGCAAAGTTA+TGG | - | chr5_4:22752076-22752095 | Msa0844330:CDS | 45.0% | |
| AAGTTGGCAATGAATGCACC+TGG | + | chr5_4:22752334-22752353 | None:intergenic | 45.0% | |
| ! | TAAAGTGGTGGGAGCAAGAA+AGG | - | chr5_4:22752385-22752404 | Msa0844330:intron | 45.0% |
| GTGTGGACCTTGCAAAGTTA+TGG | - | chr5_4:22752076-22752095 | Msa0844330:CDS | 45.0% | |
| AAGTTGGCAATGAATGCACC+TGG | + | chr5_4:22752334-22752353 | None:intergenic | 45.0% | |
| ! | TAAAGTGGTGGGAGCAAGAA+AGG | - | chr5_4:22752385-22752404 | Msa0844330:intron | 45.0% |
| !! | TCAATAAAATAATATATAAT+TGG | - | chr5_4:22752648-22752667 | Msa0844330:five_prime_UTR | 5.0% |
| !! | TCAATAAAATAATATATAAT+TGG | - | chr5_4:22752648-22752667 | Msa0844330:five_prime_UTR | 5.0% |
| TGGCAATGAATGCACCTGGA+AGG | + | chr5_4:22752330-22752349 | None:intergenic | 50.0% | |
| TGGCAATGAATGCACCTGGA+AGG | + | chr5_4:22752330-22752349 | None:intergenic | 50.0% | |
| !! | GGGAGTGTCTCAAACCTTCC+AGG | - | chr5_4:22752313-22752332 | Msa0844330:CDS | 55.0% |
| ! | AGTGGTGGGAGCAAGAAAGG+AGG | - | chr5_4:22752388-22752407 | Msa0844330:intron | 55.0% |
| !! | GGGAGTGTCTCAAACCTTCC+AGG | - | chr5_4:22752313-22752332 | Msa0844330:CDS | 55.0% |
| ! | AGTGGTGGGAGCAAGAAAGG+AGG | - | chr5_4:22752388-22752407 | Msa0844330:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_4 | gene | 22751704 | 22752707 | 22751704 | ID=Msa0844330;Name=Msa0844330 |
| chr5_4 | mRNA | 22751704 | 22752707 | 22751704 | ID=Msa0844330-mRNA-1;Parent=Msa0844330;Name=Msa0844330-mRNA-1;_AED=0.33;_eAED=0.33;_QI=134|1|1|1|1|1|3|262|126 |
| chr5_4 | exon | 22751704 | 22752097 | 22751704 | ID=Msa0844330-mRNA-1:exon:9283;Parent=Msa0844330-mRNA-1 |
| chr5_4 | exon | 22752239 | 22752361 | 22752239 | ID=Msa0844330-mRNA-1:exon:9282;Parent=Msa0844330-mRNA-1 |
| chr5_4 | exon | 22752448 | 22752707 | 22752448 | ID=Msa0844330-mRNA-1:exon:9281;Parent=Msa0844330-mRNA-1 |
| chr5_4 | five_prime_UTR | 22752574 | 22752707 | 22752574 | ID=Msa0844330-mRNA-1:five_prime_utr;Parent=Msa0844330-mRNA-1 |
| chr5_4 | CDS | 22752448 | 22752573 | 22752448 | ID=Msa0844330-mRNA-1:cds;Parent=Msa0844330-mRNA-1 |
| chr5_4 | CDS | 22752239 | 22752361 | 22752239 | ID=Msa0844330-mRNA-1:cds;Parent=Msa0844330-mRNA-1 |
| chr5_4 | CDS | 22751966 | 22752097 | 22751966 | ID=Msa0844330-mRNA-1:cds;Parent=Msa0844330-mRNA-1 |
| chr5_4 | three_prime_UTR | 22751704 | 22751965 | 22751704 | ID=Msa0844330-mRNA-1:three_prime_utr;Parent=Msa0844330-mRNA-1 |
| Gene Sequence |
| Protein sequence |
