AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0724490


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0724490 MtrunA17_Chr5g0415741 87.943 141 2 1 1 141 1 126 3.07e-87 250
Msa0724490 MtrunA17_Chr5g0415761 60.140 143 40 2 1 141 1 128 1.00e-47 150
Msa0724490 MtrunA17_Chr5g0415771 59.574 141 41 2 1 140 1 126 1.61e-47 149
Msa0724490 MtrunA17_Chr7g0216481 48.760 121 47 1 20 140 28 133 8.01e-39 127
Msa0724490 MtrunA17_Chr3g0142051 41.270 126 59 1 14 139 2 112 1.68e-32 111
Msa0724490 MtrunA17_Chr1g0155721 37.500 128 65 1 14 141 2 114 7.67e-31 107
Msa0724490 MtrunA17_Chr5g0404831 38.889 126 62 1 14 139 4 114 2.00e-30 106
Msa0724490 MtrunA17_Chr8g0393201 37.288 118 59 1 19 136 228 330 4.87e-21 86.7
Msa0724490 MtrunA17_Chr8g0334481 30.000 120 69 1 17 136 25 129 8.71e-20 79.7
Msa0724490 MtrunA17_Chr7g0245141 31.667 120 67 1 17 136 23 127 1.03e-19 79.3
Msa0724490 MtrunA17_Chr2g0280411 34.167 120 64 1 18 137 9 113 2.11e-19 78.2
Msa0724490 MtrunA17_Chr4g0042051 32.787 122 63 3 18 137 10 114 2.25e-17 73.2
Msa0724490 MtrunA17_Chr2g0319291 28.333 120 71 1 18 137 10 114 3.38e-16 69.7
Msa0724490 MtrunA17_Chr7g0249461 34.314 102 53 2 40 141 98 185 1.72e-13 64.3
Msa0724490 MtrunA17_Chr4g0052481 27.966 118 72 2 21 138 62 166 6.84e-13 62.4
Msa0724490 MtrunA17_Chr1g0201781 28.713 101 54 4 39 138 77 160 3.72e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0724490 AT5G39950.1 42.857 126 57 1 15 140 22 132 1.98e-37 124
Msa0724490 AT1G59730.1 37.762 143 71 2 1 140 1 128 4.27e-31 108
Msa0724490 AT1G69880.1 40.984 122 57 2 17 138 36 142 2.19e-30 107
Msa0724490 AT3G51030.1 37.600 125 63 1 14 138 2 111 1.29e-29 104
Msa0724490 AT1G19730.1 42.063 126 57 2 14 138 2 112 2.42e-29 103
Msa0724490 AT1G45145.1 39.669 121 58 1 18 138 5 110 3.57e-28 100
Msa0724490 AT5G42980.1 38.400 125 62 1 17 141 4 113 3.08e-27 98.2
Msa0724490 AT3G17880.1 35.593 118 61 1 19 136 271 373 3.99e-19 82.4
Msa0724490 AT3G17880.2 35.593 118 61 1 19 136 264 366 4.27e-19 82.0
Msa0724490 AT3G56420.1 32.710 107 57 1 28 134 50 141 1.00e-17 75.1
Msa0724490 AT3G56420.2 32.710 107 57 1 28 134 50 141 1.00e-17 75.1
Msa0724490 AT3G56420.3 32.710 107 57 1 28 134 50 141 1.00e-17 75.1
Msa0724490 AT3G08710.2 29.365 126 72 2 11 136 18 126 1.26e-16 71.6
Msa0724490 AT3G08710.3 29.365 126 72 2 11 136 18 126 1.26e-16 71.6
Msa0724490 AT3G08710.1 29.365 126 72 2 11 136 18 126 1.26e-16 71.6
Msa0724490 AT1G11530.1 30.000 120 69 1 18 137 2 106 2.63e-15 67.8
Msa0724490 AT5G16400.1 33.333 102 54 1 40 141 97 184 1.94e-14 67.0
Msa0724490 AT3G02730.1 35.644 101 51 2 41 141 88 174 5.35e-14 65.9
Msa0724490 AT2G40790.2 28.947 114 66 1 28 141 50 148 1.55e-13 63.9
Msa0724490 AT2G40790.1 28.947 114 66 1 28 141 50 148 1.55e-13 63.9
Msa0724490 AT1G43560.1 25.342 146 87 5 1 141 33 161 1.21e-12 62.0
Msa0724490 AT4G03520.1 37.209 86 38 3 43 127 101 171 1.91e-11 59.3

Find 37 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCCATTCCACTGCTAAA+TGG 0.238150 5_1:-23073019 Msa0724490:CDS
GCTTGATGTCGATGAGTTAA+TGG 0.243334 5_1:-23072767 Msa0724490:intron
GCAGCAAAATCTTTCATAAC+TGG 0.263663 5_1:+23072817 None:intergenic
TTATTGATTTCACAGCTAAA+TGG 0.322109 5_1:-23072864 Msa0724490:CDS
CCCACAATATAATTGGCAAC+AGG 0.356350 5_1:-23072614 Msa0724490:CDS
TGTTAATTTGTTGGGATGAT+TGG 0.356739 5_1:+23073185 None:intergenic
TTGTTGGGATGATTGGAAAT+TGG 0.378161 5_1:+23073192 None:intergenic
TATGATGTTTGTTAATTTGT+TGG 0.396039 5_1:+23073176 None:intergenic
GGGAGTGTCTCAGACCTTCC+AGG 0.411291 5_1:-23072593 Msa0724490:CDS
TAAAGTGGTGGGAGCAAGAA+AGG 0.432390 5_1:-23072521 Msa0724490:CDS
CCTGTTGCCAATTATATTGT+GGG 0.436903 5_1:+23072614 None:intergenic
TTCTTAATGAGTAGAAAAGT+TGG 0.438267 5_1:+23072559 None:intergenic
ATGATGTTTGTTAATTTGTT+GGG 0.458297 5_1:+23073177 None:intergenic
CCCTGTTGCCAATTATATTG+TGG 0.464449 5_1:+23072613 None:intergenic
GGGATGATTGGAAATTGGTT+GGG 0.476532 5_1:+23073197 None:intergenic
TGGGATGATTGGAAATTGGT+TGG 0.482987 5_1:+23073196 None:intergenic
GTGTGGACCTTGCAAAGTTA+TGG 0.487071 5_1:-23072842 Msa0724490:CDS
AGGAAAGGTTGTTGATAAAG+TGG 0.493130 5_1:-23072536 Msa0724490:CDS
TAGCAGTGGAATGGAATGTA+AGG 0.498154 5_1:+23073024 None:intergenic
CTGCAAAATACACTGATGTT+GGG 0.498670 5_1:-23072798 Msa0724490:CDS
GCTGCAAAATACACTGATGT+TGG 0.511854 5_1:-23072799 Msa0724490:CDS
TCTACTCATTAAGAAAGGAA+AGG 0.517021 5_1:-23072551 Msa0724490:CDS
ATACACAAAATTATACAAGT+TGG 0.527315 5_1:+23073076 None:intergenic
CATTCCATTTAGCAGTGGAA+TGG 0.530623 5_1:+23073015 None:intergenic
GGGATATATATAGTGTAGAA+AGG 0.532837 5_1:+23073217 None:intergenic
TGGAATGGAATGTAAGGACA+TGG 0.568326 5_1:+23073030 None:intergenic
AAGTTGGCAATGAATGCACC+TGG 0.579245 5_1:+23072575 None:intergenic
GATTTCACAGCTAAATGGTG+TGG 0.590907 5_1:-23072859 Msa0724490:CDS
AAGGTTGTTGATAAAGTGGT+GGG 0.601867 5_1:-23072532 Msa0724490:CDS
CCACAATATAATTGGCAACA+GGG 0.601984 5_1:-23072613 Msa0724490:CDS
ACTGGATCCATAACTTTGCA+AGG 0.602265 5_1:+23072835 None:intergenic
ATGAGCATTCCATTTAGCAG+TGG 0.609708 5_1:+23073010 None:intergenic
AAAGGTTGTTGATAAAGTGG+TGG 0.610487 5_1:-23072533 Msa0724490:CDS
TGGCAATGAATGCACCTGGA+AGG 0.611957 5_1:+23072579 None:intergenic
CAAGAAAGGAGGAACTTCAA+AGG 0.620784 5_1:-23072507 Msa0724490:CDS
AGTGGTGGGAGCAAGAAAGG+AGG 0.703427 5_1:-23072518 Msa0724490:CDS
GGAATGGAATGTAAGGACAT+GGG 0.736550 5_1:+23073031 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAAAGAAACTAATAAAC+TGG - chr5_1:23072721-23072740 Msa0724490:intron 15.0%
!!! TTTTAAAGAAACTAATAAAC+TGG - chr5_1:23072721-23072740 Msa0724490:intron 15.0%
!!! ATGATGTTTGTTAATTTGTT+GGG + chr5_1:23072526-23072545 None:intergenic 20.0%
!!! TATGATGTTTGTTAATTTGT+TGG + chr5_1:23072527-23072546 None:intergenic 20.0%
!! ATACACAAAATTATACAAGT+TGG + chr5_1:23072627-23072646 None:intergenic 20.0%
!!! TCTTATGCATTATTTTTCTT+TGG - chr5_1:23072804-23072823 Msa0724490:CDS 20.0%
!!! TTATTTTTCTTTGGTATAGA+TGG - chr5_1:23072813-23072832 Msa0724490:CDS 20.0%
!!! ATGATGTTTGTTAATTTGTT+GGG + chr5_1:23072526-23072545 None:intergenic 20.0%
!!! TATGATGTTTGTTAATTTGT+TGG + chr5_1:23072527-23072546 None:intergenic 20.0%
!! ATACACAAAATTATACAAGT+TGG + chr5_1:23072627-23072646 None:intergenic 20.0%
!!! TCTTATGCATTATTTTTCTT+TGG - chr5_1:23072804-23072823 Msa0724490:CDS 20.0%
!!! TTATTTTTCTTTGGTATAGA+TGG - chr5_1:23072813-23072832 Msa0724490:CDS 20.0%
!!! CATACACCTAAATCATTTTT+TGG - chr5_1:23072573-23072592 Msa0724490:CDS 25.0%
! GATGAACCAAAAAATGATTT+AGG + chr5_1:23072582-23072601 None:intergenic 25.0%
!!! TTATTGATTTCACAGCTAAA+TGG - chr5_1:23072836-23072855 Msa0724490:CDS 25.0%
! TAACAAACTAGTTAAAGTGA+AGG + chr5_1:23072974-23072993 None:intergenic 25.0%
!! TGTTTTTCCCACAATATAAT+TGG - chr5_1:23073079-23073098 Msa0724490:CDS 25.0%
! TTCTTAATGAGTAGAAAAGT+TGG + chr5_1:23073144-23073163 None:intergenic 25.0%
!! ACTTTTCTACTCATTAAGAA+AGG - chr5_1:23073144-23073163 Msa0724490:five_prime_UTR 25.0%
!!! CATACACCTAAATCATTTTT+TGG - chr5_1:23072573-23072592 Msa0724490:CDS 25.0%
! GATGAACCAAAAAATGATTT+AGG + chr5_1:23072582-23072601 None:intergenic 25.0%
!!! TTATTGATTTCACAGCTAAA+TGG - chr5_1:23072836-23072855 Msa0724490:CDS 25.0%
! TAACAAACTAGTTAAAGTGA+AGG + chr5_1:23072974-23072993 None:intergenic 25.0%
!! TGTTTTTCCCACAATATAAT+TGG - chr5_1:23073079-23073098 Msa0724490:CDS 25.0%
! TTCTTAATGAGTAGAAAAGT+TGG + chr5_1:23073144-23073163 None:intergenic 25.0%
!! ACTTTTCTACTCATTAAGAA+AGG - chr5_1:23073144-23073163 Msa0724490:five_prime_UTR 25.0%
GGGATATATATAGTGTAGAA+AGG + chr5_1:23072486-23072505 None:intergenic 30.0%
!! TGTTAATTTGTTGGGATGAT+TGG + chr5_1:23072518-23072537 None:intergenic 30.0%
TCTACTCATTAAGAAAGGAA+AGG - chr5_1:23073149-23073168 Msa0724490:five_prime_UTR 30.0%
GGGATATATATAGTGTAGAA+AGG + chr5_1:23072486-23072505 None:intergenic 30.0%
!! TGTTAATTTGTTGGGATGAT+TGG + chr5_1:23072518-23072537 None:intergenic 30.0%
TCTACTCATTAAGAAAGGAA+AGG - chr5_1:23073149-23073168 Msa0724490:five_prime_UTR 30.0%
TTGTTGGGATGATTGGAAAT+TGG + chr5_1:23072511-23072530 None:intergenic 35.0%
GCAGCAAAATCTTTCATAAC+TGG + chr5_1:23072886-23072905 None:intergenic 35.0%
CTGCAAAATACACTGATGTT+GGG - chr5_1:23072902-23072921 Msa0724490:intron 35.0%
CCTGTTGCCAATTATATTGT+GGG + chr5_1:23073089-23073108 None:intergenic 35.0%
CCACAATATAATTGGCAACA+GGG - chr5_1:23073087-23073106 Msa0724490:exon 35.0%
! AGGAAAGGTTGTTGATAAAG+TGG - chr5_1:23073164-23073183 Msa0724490:five_prime_UTR 35.0%
! AAAGGTTGTTGATAAAGTGG+TGG - chr5_1:23073167-23073186 Msa0724490:five_prime_UTR 35.0%
! AAGGTTGTTGATAAAGTGGT+GGG - chr5_1:23073168-23073187 Msa0724490:five_prime_UTR 35.0%
TTGTTGGGATGATTGGAAAT+TGG + chr5_1:23072511-23072530 None:intergenic 35.0%
GCAGCAAAATCTTTCATAAC+TGG + chr5_1:23072886-23072905 None:intergenic 35.0%
CTGCAAAATACACTGATGTT+GGG - chr5_1:23072902-23072921 Msa0724490:intron 35.0%
CCTGTTGCCAATTATATTGT+GGG + chr5_1:23073089-23073108 None:intergenic 35.0%
CCACAATATAATTGGCAACA+GGG - chr5_1:23073087-23073106 Msa0724490:exon 35.0%
! AGGAAAGGTTGTTGATAAAG+TGG - chr5_1:23073164-23073183 Msa0724490:five_prime_UTR 35.0%
! AAAGGTTGTTGATAAAGTGG+TGG - chr5_1:23073167-23073186 Msa0724490:five_prime_UTR 35.0%
! AAGGTTGTTGATAAAGTGGT+GGG - chr5_1:23073168-23073187 Msa0724490:five_prime_UTR 35.0%
GGGATGATTGGAAATTGGTT+GGG + chr5_1:23072506-23072525 None:intergenic 40.0%
TGGGATGATTGGAAATTGGT+TGG + chr5_1:23072507-23072526 None:intergenic 40.0%
GGAATGGAATGTAAGGACAT+GGG + chr5_1:23072672-23072691 None:intergenic 40.0%
TGGAATGGAATGTAAGGACA+TGG + chr5_1:23072673-23072692 None:intergenic 40.0%
TAGCAGTGGAATGGAATGTA+AGG + chr5_1:23072679-23072698 None:intergenic 40.0%
CATTCCATTCCACTGCTAAA+TGG - chr5_1:23072681-23072700 Msa0724490:intron 40.0%
CATTCCATTTAGCAGTGGAA+TGG + chr5_1:23072688-23072707 None:intergenic 40.0%
ATGAGCATTCCATTTAGCAG+TGG + chr5_1:23072693-23072712 None:intergenic 40.0%
GATTTCACAGCTAAATGGTG+TGG - chr5_1:23072841-23072860 Msa0724490:CDS 40.0%
ACTGGATCCATAACTTTGCA+AGG + chr5_1:23072868-23072887 None:intergenic 40.0%
GCTGCAAAATACACTGATGT+TGG - chr5_1:23072901-23072920 Msa0724490:intron 40.0%
GCTTGATGTCGATGAGTTAA+TGG - chr5_1:23072933-23072952 Msa0724490:intron 40.0%
ACAAATCAGTTCTCAAGTGC+TGG + chr5_1:23073062-23073081 None:intergenic 40.0%
CCCACAATATAATTGGCAAC+AGG - chr5_1:23073086-23073105 Msa0724490:CDS 40.0%
CCCTGTTGCCAATTATATTG+TGG + chr5_1:23073090-23073109 None:intergenic 40.0%
CAAGAAAGGAGGAACTTCAA+AGG - chr5_1:23073193-23073212 Msa0724490:five_prime_UTR 40.0%
GGGATGATTGGAAATTGGTT+GGG + chr5_1:23072506-23072525 None:intergenic 40.0%
TGGGATGATTGGAAATTGGT+TGG + chr5_1:23072507-23072526 None:intergenic 40.0%
GGAATGGAATGTAAGGACAT+GGG + chr5_1:23072672-23072691 None:intergenic 40.0%
TGGAATGGAATGTAAGGACA+TGG + chr5_1:23072673-23072692 None:intergenic 40.0%
TAGCAGTGGAATGGAATGTA+AGG + chr5_1:23072679-23072698 None:intergenic 40.0%
CATTCCATTCCACTGCTAAA+TGG - chr5_1:23072681-23072700 Msa0724490:intron 40.0%
CATTCCATTTAGCAGTGGAA+TGG + chr5_1:23072688-23072707 None:intergenic 40.0%
ATGAGCATTCCATTTAGCAG+TGG + chr5_1:23072693-23072712 None:intergenic 40.0%
GATTTCACAGCTAAATGGTG+TGG - chr5_1:23072841-23072860 Msa0724490:CDS 40.0%
ACTGGATCCATAACTTTGCA+AGG + chr5_1:23072868-23072887 None:intergenic 40.0%
GCTGCAAAATACACTGATGT+TGG - chr5_1:23072901-23072920 Msa0724490:intron 40.0%
GCTTGATGTCGATGAGTTAA+TGG - chr5_1:23072933-23072952 Msa0724490:intron 40.0%
ACAAATCAGTTCTCAAGTGC+TGG + chr5_1:23073062-23073081 None:intergenic 40.0%
CCCACAATATAATTGGCAAC+AGG - chr5_1:23073086-23073105 Msa0724490:CDS 40.0%
CCCTGTTGCCAATTATATTG+TGG + chr5_1:23073090-23073109 None:intergenic 40.0%
CAAGAAAGGAGGAACTTCAA+AGG - chr5_1:23073193-23073212 Msa0724490:five_prime_UTR 40.0%
GTGTGGACCTTGCAAAGTTA+TGG - chr5_1:23072858-23072877 Msa0724490:CDS 45.0%
AAGTTGGCAATGAATGCACC+TGG + chr5_1:23073128-23073147 None:intergenic 45.0%
! TAAAGTGGTGGGAGCAAGAA+AGG - chr5_1:23073179-23073198 Msa0724490:five_prime_UTR 45.0%
GTGTGGACCTTGCAAAGTTA+TGG - chr5_1:23072858-23072877 Msa0724490:CDS 45.0%
AAGTTGGCAATGAATGCACC+TGG + chr5_1:23073128-23073147 None:intergenic 45.0%
! TAAAGTGGTGGGAGCAAGAA+AGG - chr5_1:23073179-23073198 Msa0724490:five_prime_UTR 45.0%
TGGCAATGAATGCACCTGGA+AGG + chr5_1:23073124-23073143 None:intergenic 50.0%
TGGCAATGAATGCACCTGGA+AGG + chr5_1:23073124-23073143 None:intergenic 50.0%
! AGTGGTGGGAGCAAGAAAGG+AGG - chr5_1:23073182-23073201 Msa0724490:five_prime_UTR 55.0%
! AGTGGTGGGAGCAAGAAAGG+AGG - chr5_1:23073182-23073201 Msa0724490:five_prime_UTR 55.0%
!! GGGAGTGTCTCAGACCTTCC+AGG - chr5_1:23073107-23073126 Msa0724490:five_prime_UTR 60.0%
!! GGGAGTGTCTCAGACCTTCC+AGG - chr5_1:23073107-23073126 Msa0724490:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 23072483 23073239 23072483 ID=Msa0724490;Name=Msa0724490
chr5_1 mRNA 23072483 23073239 23072483 ID=Msa0724490-mRNA-1;Parent=Msa0724490;Name=Msa0724490-mRNA-1;_AED=0.34;_eAED=0.39;_QI=134|0.5|0.66|1|1|1|3|0|141
chr5_1 exon 23072483 23072659 23072483 ID=Msa0724490-mRNA-1:exon:8653;Parent=Msa0724490-mRNA-1
chr5_1 exon 23072768 23072890 23072768 ID=Msa0724490-mRNA-1:exon:8652;Parent=Msa0724490-mRNA-1
chr5_1 exon 23072980 23073239 23072980 ID=Msa0724490-mRNA-1:exon:8651;Parent=Msa0724490-mRNA-1
chr5_1 five_prime_UTR 23073106 23073239 23073106 ID=Msa0724490-mRNA-1:five_prime_utr;Parent=Msa0724490-mRNA-1
chr5_1 CDS 23072980 23073105 23072980 ID=Msa0724490-mRNA-1:cds;Parent=Msa0724490-mRNA-1
chr5_1 CDS 23072768 23072890 23072768 ID=Msa0724490-mRNA-1:cds;Parent=Msa0724490-mRNA-1
chr5_1 CDS 23072483 23072659 23072483 ID=Msa0724490-mRNA-1:cds;Parent=Msa0724490-mRNA-1
Gene Sequence

>Msa0724490

ATGAGTTCCAACTTGTATAATTTTGTGTATGTTGATAGATCAACAGAATCATCCCATGTCCTTACATTCCATTCCACTGCTAAATGGAATGCTCATTTTGAAGCTTTTAAAGAAACTAATAAACTGATGGTTATTGATTTCACAGCTAAATGGTGTGGACCTTGCAAAGTTATGGATCCAGTTATGAAAGATTTTGCTGCAAAATACACTGATGTTGGGTTCATTAAGCTTGATGTCGATGAGTTAATGCACTTGAGAACTGATTTGTTTTTCCCACAATATAATTGGCAACAGGGAGTGTCTCAGACCTTCCAGGTGCATTCATTGCCAACTTTTCTACTCATTAAGAAAGGAAAGGTTGTTGATAAAGTGGTGGGAGCAAGAAAGGAGGAACTTCAAAGGATGATTGAAAAACAAAGAAACTAA

Protein sequence

>Msa0724490

MSSNLYNFVYVDRSTESSHVLTFHSTAKWNAHFEAFKETNKLMVIDFTAKWCGPCKVMDPVMKDFAAKYTDVGFIKLDVDELMHLRTDLFFPQYNWQQGVSQTFQVHSLPTFLLIKKGKVVDKVVGARKEELQRMIEKQRN*