AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0765020


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0765020 MtrunA17_Chr5g0415741 96.825 126 4 0 1 126 1 126 2.88e-91 259
Msa0765020 MtrunA17_Chr5g0415771 66.667 126 41 1 1 125 1 126 3.10e-52 160
Msa0765020 MtrunA17_Chr5g0415761 64.844 128 43 1 1 126 1 128 4.89e-51 158
Msa0765020 MtrunA17_Chr7g0216481 54.717 106 48 0 20 125 28 133 8.24e-43 137
Msa0765020 MtrunA17_Chr3g0142051 46.847 111 59 0 14 124 2 112 2.56e-36 120
Msa0765020 MtrunA17_Chr1g0155721 40.708 113 67 0 14 126 2 114 2.19e-33 113
Msa0765020 MtrunA17_Chr5g0404831 42.342 111 64 0 14 124 4 114 3.65e-33 112
Msa0765020 MtrunA17_Chr8g0393201 41.346 104 61 0 18 121 227 330 2.20e-24 95.1
Msa0765020 MtrunA17_Chr8g0334481 32.381 105 71 0 17 121 25 129 2.38e-22 85.5
Msa0765020 MtrunA17_Chr7g0245141 34.286 105 69 0 17 121 23 127 2.74e-22 85.5
Msa0765020 MtrunA17_Chr2g0280411 37.143 105 66 0 18 122 9 113 7.44e-22 84.0
Msa0765020 MtrunA17_Chr4g0042051 35.514 107 65 2 18 122 10 114 6.43e-20 79.0
Msa0765020 MtrunA17_Chr2g0319291 30.476 105 73 0 18 122 10 114 1.44e-18 75.5
Msa0765020 MtrunA17_Chr1g0201781 32.222 90 59 2 39 126 77 166 1.19e-14 66.6
Msa0765020 MtrunA17_Chr7g0249461 36.364 88 55 1 40 126 98 185 4.25e-14 65.5
Msa0765020 MtrunA17_Chr8g0360031 34.091 88 56 2 38 123 87 174 4.41e-14 65.1
Msa0765020 MtrunA17_Chr4g0052481 32.927 82 53 1 44 123 85 166 9.30e-14 64.3
Msa0765020 MtrunA17_Chr3g0125821 31.313 99 63 3 27 123 76 171 9.57e-14 64.3
Msa0765020 MtrunA17_Chr2g0317731 34.091 88 56 2 38 123 84 171 3.11e-13 62.8
Msa0765020 MtrunA17_Chr1g0211871 32.911 79 49 2 38 115 84 159 3.43e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0765020 AT5G39950.1 46.847 111 59 0 15 125 22 132 6.66e-41 132
Msa0765020 AT1G69880.1 46.729 107 57 0 17 123 36 142 8.73e-35 117
Msa0765020 AT1G59730.1 39.844 128 74 1 1 125 1 128 9.59e-34 114
Msa0765020 AT3G51030.1 40.909 110 65 0 14 123 2 111 4.33e-32 110
Msa0765020 AT1G19730.1 45.536 112 60 1 14 124 2 113 1.49e-31 108
Msa0765020 AT1G45145.1 42.056 107 62 0 18 124 5 111 3.46e-30 105
Msa0765020 AT5G42980.1 41.818 110 64 0 17 126 4 113 9.04e-30 104
Msa0765020 AT3G17880.2 39.806 103 62 0 19 121 264 366 4.31e-22 89.7
Msa0765020 AT3G17880.1 39.806 103 62 0 19 121 271 373 5.20e-22 89.7
Msa0765020 AT3G56420.1 35.870 92 59 0 28 119 50 141 3.54e-20 80.9
Msa0765020 AT3G56420.2 35.870 92 59 0 28 119 50 141 3.54e-20 80.9
Msa0765020 AT3G56420.3 35.870 92 59 0 28 119 50 141 3.54e-20 80.9
Msa0765020 AT3G08710.2 31.532 111 74 1 11 121 18 126 1.94e-19 78.6
Msa0765020 AT3G08710.3 31.532 111 74 1 11 121 18 126 1.94e-19 78.6
Msa0765020 AT3G08710.1 31.532 111 74 1 11 121 18 126 1.94e-19 78.6
Msa0765020 AT1G11530.1 32.381 105 71 0 18 122 2 106 8.64e-18 73.6
Msa0765020 AT2G40790.2 33.696 92 61 0 28 119 50 141 3.70e-16 70.5
Msa0765020 AT2G40790.1 33.696 92 61 0 28 119 50 141 3.70e-16 70.5
Msa0765020 AT1G43560.1 28.906 128 85 4 1 122 33 160 1.46e-15 69.3
Msa0765020 AT5G16400.1 37.500 88 54 1 40 126 97 184 2.23e-15 68.9
Msa0765020 AT3G02730.1 39.080 87 52 1 41 126 88 174 4.12e-15 68.2
Msa0765020 AT4G03520.1 38.554 83 49 2 43 123 101 183 8.80e-15 67.4
Msa0765020 AT1G03680.1 38.272 81 48 2 45 123 97 177 2.03e-14 66.6
Msa0765020 AT1G31020.1 30.833 120 77 2 6 123 41 156 3.94e-14 65.1
Msa0765020 AT1G76760.1 28.421 95 61 3 33 120 70 164 9.32e-14 64.7
Msa0765020 AT2G35010.2 36.250 80 47 2 47 123 113 191 9.57e-13 62.4
Msa0765020 AT2G35010.1 36.250 80 47 2 47 123 113 191 9.57e-13 62.4
Msa0765020 AT1G50320.1 36.709 79 46 2 38 115 85 160 3.04e-12 60.8
Msa0765020 AT1G52990.1 31.868 91 60 2 34 123 222 311 2.07e-11 59.7

Find 31 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCCATTCCACTGCTAAA+TGG 0.238150 5_2:-25505359 Msa0765020:CDS
AATCAATAAACTAATATAAT+TGG 0.246269 5_2:-25504536 None:intergenic
GCAGCAAAATCTTTCATAAC+TGG 0.263663 5_2:+25505157 None:intergenic
GCTTGATGTTGATGAGTTAT+TGG 0.264813 5_2:-25505107 Msa0765020:intron
TTATTGATTTCACAGCTAAA+TGG 0.322109 5_2:-25505204 Msa0765020:CDS
CTCATTCTTCTTTGATTACA+AGG 0.356202 5_2:-25504716 Msa0765020:three_prime_UTR
TGTTAATTTGTTGGGATGAT+TGG 0.356739 5_2:+25505525 None:intergenic
TTGTTGGGATGATTGGAAAT+TGG 0.378161 5_2:+25505532 None:intergenic
TATGATGTTTGTTAATTTGT+TGG 0.397122 5_2:+25505516 None:intergenic
TTCTTAATGAGTAGAAAAGT+TGG 0.438267 5_2:+25504856 None:intergenic
ATGATGTTTGTTAATTTGTT+GGG 0.449780 5_2:+25505517 None:intergenic
GGGATGATTGGAAATTGGTT+GGG 0.476532 5_2:+25505537 None:intergenic
AGGAAAAGTTGTTGATAAAG+TGG 0.478902 5_2:-25504833 Msa0765020:CDS
TAAAGTGGTGGGAGTAAGAA+AGG 0.479129 5_2:-25504818 Msa0765020:CDS
GGGAGTTTCTCAAACATTCC+AGG 0.482800 5_2:-25504890 Msa0765020:CDS
TGGGATGATTGGAAATTGGT+TGG 0.482987 5_2:+25505536 None:intergenic
GTGTGGACCTTGCAAAGTTA+TGG 0.487071 5_2:-25505182 Msa0765020:CDS
TAGCAGTGGAATGGAATGTA+AGG 0.498154 5_2:+25505364 None:intergenic
ATACACAAAATTATACAAGT+TGG 0.527315 5_2:+25505416 None:intergenic
CATTCCATTTAGCAGTGGAA+TGG 0.530623 5_2:+25505355 None:intergenic
GGGATATATATAGTGTAGAA+AGG 0.532837 5_2:+25505557 None:intergenic
TGGAATGGAATGTAAGGACA+TGG 0.568326 5_2:+25505370 None:intergenic
AAAAGTTGTTGATAAAGTGG+TGG 0.577415 5_2:-25504830 Msa0765020:CDS
AAGTTGGCAATGAATGCACC+TGG 0.579245 5_2:+25504872 None:intergenic
GATTTCACAGCTAAATGGTG+TGG 0.590907 5_2:-25505199 Msa0765020:CDS
TCATTCTTCTTTGATTACAA+GGG 0.593689 5_2:-25504715 Msa0765020:three_prime_UTR
AAAGTTGTTGATAAAGTGGT+GGG 0.597105 5_2:-25504829 Msa0765020:CDS
ACTGGATCCATAACTTTGCA+AGG 0.602265 5_2:+25505175 None:intergenic
ATGAGCATTCCATTTAGCAG+TGG 0.609708 5_2:+25505350 None:intergenic
TAAGAAAGGAGCAACTACAA+AGG 0.660815 5_2:-25504804 Msa0765020:CDS
GGAATGGAATGTAAGGACAT+GGG 0.736550 5_2:+25505371 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAATAGCATTTATAAT+AGG + chr5_2:25505122-25505141 None:intergenic 10.0%
!! AAAATAATAGCATTTATAAT+AGG + chr5_2:25505122-25505141 None:intergenic 10.0%
!!! TTTTAAAGAAACTAATAAAC+TGG - chr5_2:25504775-25504794 Msa0765020:exon 15.0%
!! ACTCAAAAAATAGAAAATAT+AGG - chr5_2:25505448-25505467 Msa0765020:five_prime_UTR 15.0%
!!! TTTTAAAGAAACTAATAAAC+TGG - chr5_2:25504775-25504794 Msa0765020:exon 15.0%
!! ACTCAAAAAATAGAAAATAT+AGG - chr5_2:25505448-25505467 Msa0765020:five_prime_UTR 15.0%
!!! ATGATGTTTGTTAATTTGTT+GGG + chr5_2:25504580-25504599 None:intergenic 20.0%
!!! TATGATGTTTGTTAATTTGT+TGG + chr5_2:25504581-25504600 None:intergenic 20.0%
!! ATACACAAAATTATACAAGT+TGG + chr5_2:25504681-25504700 None:intergenic 20.0%
!!! TCTTATGTAGTATTTTTCTT+TGG - chr5_2:25504858-25504877 Msa0765020:CDS 20.0%
!!! TGTTTTTTCCACAATATAAA+TGG - chr5_2:25505176-25505195 Msa0765020:CDS 20.0%
!!! TCATTCTTTTATCATTAAAG+AGG - chr5_2:25505473-25505492 Msa0765020:five_prime_UTR 20.0%
!!! ATGATGTTTGTTAATTTGTT+GGG + chr5_2:25504580-25504599 None:intergenic 20.0%
!!! TATGATGTTTGTTAATTTGT+TGG + chr5_2:25504581-25504600 None:intergenic 20.0%
!! ATACACAAAATTATACAAGT+TGG + chr5_2:25504681-25504700 None:intergenic 20.0%
!!! TCTTATGTAGTATTTTTCTT+TGG - chr5_2:25504858-25504877 Msa0765020:CDS 20.0%
!!! TGTTTTTTCCACAATATAAA+TGG - chr5_2:25505176-25505195 Msa0765020:CDS 20.0%
!!! TCATTCTTTTATCATTAAAG+AGG - chr5_2:25505473-25505492 Msa0765020:five_prime_UTR 20.0%
!!! CATACACCTAAATCATTTTT+TGG - chr5_2:25504627-25504646 Msa0765020:three_prime_UTR 25.0%
! GATGAACCAAAAAATGATTT+AGG + chr5_2:25504636-25504655 None:intergenic 25.0%
!! GTATTTTTCTTTGGTATAGA+TGG - chr5_2:25504867-25504886 Msa0765020:CDS 25.0%
!!! TTATTGATTTCACAGCTAAA+TGG - chr5_2:25504890-25504909 Msa0765020:CDS 25.0%
! TAACAAACTAGTTAAAGTGA+AGG + chr5_2:25505028-25505047 None:intergenic 25.0%
! TTCTTAATGAGTAGAAAAGT+TGG + chr5_2:25505241-25505260 None:intergenic 25.0%
!! ACTTTTCTACTCATTAAGAA+AGG - chr5_2:25505241-25505260 Msa0765020:intron 25.0%
! TCATTCTTCTTTGATTACAA+GGG - chr5_2:25505379-25505398 Msa0765020:CDS 25.0%
!!! CATACACCTAAATCATTTTT+TGG - chr5_2:25504627-25504646 Msa0765020:three_prime_UTR 25.0%
! GATGAACCAAAAAATGATTT+AGG + chr5_2:25504636-25504655 None:intergenic 25.0%
!! GTATTTTTCTTTGGTATAGA+TGG - chr5_2:25504867-25504886 Msa0765020:CDS 25.0%
!!! TTATTGATTTCACAGCTAAA+TGG - chr5_2:25504890-25504909 Msa0765020:CDS 25.0%
! TAACAAACTAGTTAAAGTGA+AGG + chr5_2:25505028-25505047 None:intergenic 25.0%
! TTCTTAATGAGTAGAAAAGT+TGG + chr5_2:25505241-25505260 None:intergenic 25.0%
!! ACTTTTCTACTCATTAAGAA+AGG - chr5_2:25505241-25505260 Msa0765020:intron 25.0%
! TCATTCTTCTTTGATTACAA+GGG - chr5_2:25505379-25505398 Msa0765020:CDS 25.0%
GGGATATATATAGTGTAGAA+AGG + chr5_2:25504540-25504559 None:intergenic 30.0%
!! TGTTAATTTGTTGGGATGAT+TGG + chr5_2:25504572-25504591 None:intergenic 30.0%
AATTAAACCTGGCATAAAGT+TGG + chr5_2:25505062-25505081 None:intergenic 30.0%
! AGGAAAAGTTGTTGATAAAG+TGG - chr5_2:25505261-25505280 Msa0765020:intron 30.0%
! AAAAGTTGTTGATAAAGTGG+TGG - chr5_2:25505264-25505283 Msa0765020:intron 30.0%
! AAAGTTGTTGATAAAGTGGT+GGG - chr5_2:25505265-25505284 Msa0765020:intron 30.0%
CTCATTCTTCTTTGATTACA+AGG - chr5_2:25505378-25505397 Msa0765020:CDS 30.0%
GGGATATATATAGTGTAGAA+AGG + chr5_2:25504540-25504559 None:intergenic 30.0%
!! TGTTAATTTGTTGGGATGAT+TGG + chr5_2:25504572-25504591 None:intergenic 30.0%
AATTAAACCTGGCATAAAGT+TGG + chr5_2:25505062-25505081 None:intergenic 30.0%
! AGGAAAAGTTGTTGATAAAG+TGG - chr5_2:25505261-25505280 Msa0765020:intron 30.0%
! AAAAGTTGTTGATAAAGTGG+TGG - chr5_2:25505264-25505283 Msa0765020:intron 30.0%
! AAAGTTGTTGATAAAGTGGT+GGG - chr5_2:25505265-25505284 Msa0765020:intron 30.0%
CTCATTCTTCTTTGATTACA+AGG - chr5_2:25505378-25505397 Msa0765020:CDS 30.0%
TTGTTGGGATGATTGGAAAT+TGG + chr5_2:25504565-25504584 None:intergenic 35.0%
GCAGCAAAATCTTTCATAAC+TGG + chr5_2:25504940-25504959 None:intergenic 35.0%
! GCTTGATGTTGATGAGTTAT+TGG - chr5_2:25504987-25505006 Msa0765020:intron 35.0%
TGTAAAACCAACTTTATGCC+AGG - chr5_2:25505052-25505071 Msa0765020:intron 35.0%
ACCAACTGAGTAATTAAACC+TGG + chr5_2:25505073-25505092 None:intergenic 35.0%
TCCACAATATAAATGGCAAC+AGG - chr5_2:25505183-25505202 Msa0765020:CDS 35.0%
CCACAATATAAATGGCAACA+GGG - chr5_2:25505184-25505203 Msa0765020:CDS 35.0%
TAAGAAAGGAGCAACTACAA+AGG - chr5_2:25505290-25505309 Msa0765020:intron 35.0%
TTGTTGGGATGATTGGAAAT+TGG + chr5_2:25504565-25504584 None:intergenic 35.0%
GCAGCAAAATCTTTCATAAC+TGG + chr5_2:25504940-25504959 None:intergenic 35.0%
! GCTTGATGTTGATGAGTTAT+TGG - chr5_2:25504987-25505006 Msa0765020:intron 35.0%
TGTAAAACCAACTTTATGCC+AGG - chr5_2:25505052-25505071 Msa0765020:intron 35.0%
ACCAACTGAGTAATTAAACC+TGG + chr5_2:25505073-25505092 None:intergenic 35.0%
TCCACAATATAAATGGCAAC+AGG - chr5_2:25505183-25505202 Msa0765020:CDS 35.0%
CCACAATATAAATGGCAACA+GGG - chr5_2:25505184-25505203 Msa0765020:CDS 35.0%
TAAGAAAGGAGCAACTACAA+AGG - chr5_2:25505290-25505309 Msa0765020:intron 35.0%
GGGATGATTGGAAATTGGTT+GGG + chr5_2:25504560-25504579 None:intergenic 40.0%
TGGGATGATTGGAAATTGGT+TGG + chr5_2:25504561-25504580 None:intergenic 40.0%
GGAATGGAATGTAAGGACAT+GGG + chr5_2:25504726-25504745 None:intergenic 40.0%
TGGAATGGAATGTAAGGACA+TGG + chr5_2:25504727-25504746 None:intergenic 40.0%
TAGCAGTGGAATGGAATGTA+AGG + chr5_2:25504733-25504752 None:intergenic 40.0%
CATTCCATTCCACTGCTAAA+TGG - chr5_2:25504735-25504754 Msa0765020:three_prime_UTR 40.0%
CATTCCATTTAGCAGTGGAA+TGG + chr5_2:25504742-25504761 None:intergenic 40.0%
ATGAGCATTCCATTTAGCAG+TGG + chr5_2:25504747-25504766 None:intergenic 40.0%
GATTTCACAGCTAAATGGTG+TGG - chr5_2:25504895-25504914 Msa0765020:intron 40.0%
ACTGGATCCATAACTTTGCA+AGG + chr5_2:25504922-25504941 None:intergenic 40.0%
GCCAGGTTTAATTACTCAGT+TGG - chr5_2:25505069-25505088 Msa0765020:intron 40.0%
! CCCTGTTGCCATTTATATTG+TGG + chr5_2:25505187-25505206 None:intergenic 40.0%
! TAAAGTGGTGGGAGTAAGAA+AGG - chr5_2:25505276-25505295 Msa0765020:intron 40.0%
GGGATGATTGGAAATTGGTT+GGG + chr5_2:25504560-25504579 None:intergenic 40.0%
TGGGATGATTGGAAATTGGT+TGG + chr5_2:25504561-25504580 None:intergenic 40.0%
GGAATGGAATGTAAGGACAT+GGG + chr5_2:25504726-25504745 None:intergenic 40.0%
TGGAATGGAATGTAAGGACA+TGG + chr5_2:25504727-25504746 None:intergenic 40.0%
TAGCAGTGGAATGGAATGTA+AGG + chr5_2:25504733-25504752 None:intergenic 40.0%
CATTCCATTCCACTGCTAAA+TGG - chr5_2:25504735-25504754 Msa0765020:three_prime_UTR 40.0%
CATTCCATTTAGCAGTGGAA+TGG + chr5_2:25504742-25504761 None:intergenic 40.0%
ATGAGCATTCCATTTAGCAG+TGG + chr5_2:25504747-25504766 None:intergenic 40.0%
GATTTCACAGCTAAATGGTG+TGG - chr5_2:25504895-25504914 Msa0765020:intron 40.0%
ACTGGATCCATAACTTTGCA+AGG + chr5_2:25504922-25504941 None:intergenic 40.0%
GCCAGGTTTAATTACTCAGT+TGG - chr5_2:25505069-25505088 Msa0765020:intron 40.0%
! CCCTGTTGCCATTTATATTG+TGG + chr5_2:25505187-25505206 None:intergenic 40.0%
! TAAAGTGGTGGGAGTAAGAA+AGG - chr5_2:25505276-25505295 Msa0765020:intron 40.0%
GTGTGGACCTTGCAAAGTTA+TGG - chr5_2:25504912-25504931 Msa0765020:intron 45.0%
GGGAGTTTCTCAAACATTCC+AGG - chr5_2:25505204-25505223 Msa0765020:CDS 45.0%
AAGTTGGCAATGAATGCACC+TGG + chr5_2:25505225-25505244 None:intergenic 45.0%
GTGTGGACCTTGCAAAGTTA+TGG - chr5_2:25504912-25504931 Msa0765020:intron 45.0%
GGGAGTTTCTCAAACATTCC+AGG - chr5_2:25505204-25505223 Msa0765020:CDS 45.0%
AAGTTGGCAATGAATGCACC+TGG + chr5_2:25505225-25505244 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 25504537 25505579 25504537 ID=Msa0765020;Name=Msa0765020
chr5_2 mRNA 25504537 25505579 25504537 ID=Msa0765020-mRNA-1;Parent=Msa0765020;Name=Msa0765020-mRNA-1;_AED=0.35;_eAED=0.35;_QI=134|1|1|1|1|1|3|243|126
chr5_2 exon 25504537 25504911 25504537 ID=Msa0765020-mRNA-1:exon:10293;Parent=Msa0765020-mRNA-1
chr5_2 exon 25505108 25505230 25505108 ID=Msa0765020-mRNA-1:exon:10292;Parent=Msa0765020-mRNA-1
chr5_2 exon 25505320 25505579 25505320 ID=Msa0765020-mRNA-1:exon:10291;Parent=Msa0765020-mRNA-1
chr5_2 five_prime_UTR 25505446 25505579 25505446 ID=Msa0765020-mRNA-1:five_prime_utr;Parent=Msa0765020-mRNA-1
chr5_2 CDS 25505320 25505445 25505320 ID=Msa0765020-mRNA-1:cds;Parent=Msa0765020-mRNA-1
chr5_2 CDS 25505108 25505230 25505108 ID=Msa0765020-mRNA-1:cds;Parent=Msa0765020-mRNA-1
chr5_2 CDS 25504780 25504911 25504780 ID=Msa0765020-mRNA-1:cds;Parent=Msa0765020-mRNA-1
chr5_2 three_prime_UTR 25504537 25504779 25504537 ID=Msa0765020-mRNA-1:three_prime_utr;Parent=Msa0765020-mRNA-1
Gene Sequence

>Msa0765020

ATGAGTTCCAACTTGTATAATTTTGTGTATGTTGATAGATCAACAGAATCATCCCATGTCCTTACATTCCATTCCACTGCTAAATGGAATGCTCATTTTGAAGCTTTTAAAGAAACTAATAAACTGATGGTTATTGATTTCACAGCTAAATGGTGTGGACCTTGCAAAGTTATGGATCCAGTTATGAAAGATTTTGCTGCAAAATACACTGATGTTGAGTTCATTAAGCTTGATGTTGATGAGTTATTGGGAGTTTCTCAAACATTCCAGGTGCATTCATTGCCAACTTTTCTACTCATTAAGAAAGGAAAAGTTGTTGATAAAGTGGTGGGAGTAAGAAAGGAGCAACTACAAAGGATGATTGAAAAGCAAAGAAACTAA

Protein sequence

>Msa0765020

MSSNLYNFVYVDRSTESSHVLTFHSTAKWNAHFEAFKETNKLMVIDFTAKWCGPCKVMDPVMKDFAAKYTDVEFIKLDVDELLGVSQTFQVHSLPTFLLIKKGKVVDKVVGVRKEQLQRMIEKQRN*