AlfalfaGEDB | Msa1311070

M. sativa cultivar zhongmu-4 / Msa1311070

KEGG: K19613
GO: -
PFAM: PF18052.4

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1311070	sp\|Q7XBQ9\|RGA2_SOLBU	35.930	629	348	13	1	624	1	579	5.34e-110	367

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1311070	A0A072THN4	78.282	1013	129	10	1	1010	1	925	0.0	1512

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0032130	Msa1311070	0.804163	2.464362e-49	-8.615850e-47
Msa0032390	Msa1311070	0.806890	6.602425e-50	-8.615850e-47
Msa0048950	Msa1311070	0.827864	1.236445e-54	-8.615850e-47
Msa0059880	Msa1311070	0.832639	8.429599e-56	-8.615850e-47
Msa0072030	Msa1311070	0.802867	4.576061e-49	-8.615850e-47
Msa0073270	Msa1311070	0.803706	3.066822e-49	-8.615850e-47
Msa0084190	Msa1311070	0.806096	9.712055e-50	-8.615850e-47
Msa0099890	Msa1311070	0.800468	1.421275e-48	-8.615850e-47
Msa0131460	Msa1311070	0.805004	1.645775e-49	-8.615850e-47
Msa0147680	Msa1311070	0.809065	2.274246e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1311070	MtrunA17_Chr6g0475491	87.108	830	104	3	1	828	1	829	1434
Msa1311070	MtrunA17_Chr7g0218941	65.660	993	308	8	1	987	1	966	1229
Msa1311070	MtrunA17_Chr8g0346831	62.168	978	341	12	1	975	1	952	1147
Msa1311070	MtrunA17_Chr6g0471941	59.334	991	369	14	1	986	1	962	1099
Msa1311070	MtrunA17_Chr8g0338391	59.315	993	359	19	1	986	1	955	1057
Msa1311070	MtrunA17_Chr8g0338261	57.027	989	365	12	1	986	1	932	1035
Msa1311070	MtrunA17_Chr8g0352211	55.477	995	409	15	1	991	1001	1965	984
Msa1311070	MtrunA17_Chr8g0337891	63.832	788	258	10	1	786	1	763	963
Msa1311070	MtrunA17_Chr8g0338621	73.920	648	162	4	1	647	1	642	947
Msa1311070	MtrunA17_Chr8g0338251	59.559	816	261	8	1	814	1	749	893

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1311070	AT3G14470.1	32.301	678	405	18	1	648	4	657	1.07e-92	318
Msa1311070	AT3G14470.2	32.301	678	405	18	1	648	4	657	1.07e-92	318
Msa1311070	AT3G14460.1	31.406	640	395	16	35	651	36	654	3.06e-79	283
Msa1311070	AT3G46710.1	25.503	894	540	38	8	861	4	811	2.02e-53	202
Msa1311070	AT1G53350.1	28.437	531	327	16	1	506	1	503	2.23e-53	202
Msa1311070	AT5G43470.3	29.140	628	365	24	23	604	19	612	1.42e-52	200
Msa1311070	AT5G43470.2	29.140	628	365	24	23	604	19	612	1.42e-52	200
Msa1311070	AT5G43470.4	29.140	628	365	24	23	604	19	612	1.42e-52	200
Msa1311070	AT5G43470.1	29.140	628	365	24	23	604	19	612	1.42e-52	200
Msa1311070	AT3G50950.2	28.119	505	327	15	22	509	17	502	6.90e-52	197

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Find 184 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCATGGTGTGCAAAAT+TGG	0.177094	8_4:-7753925	Msa1311070:CDS
AAAATTAACAGTAGTTTAAG+AGG	0.352261	8_4:+7751083	None:intergenic
AAACAGTGTCTGGAAGATAT+TGG	0.426366	8_4:-7752726	Msa1311070:CDS
AAAGACAGCTCTTGCTCAAT+TGG	0.372077	8_4:-7753492	Msa1311070:CDS
AAAGTAATACTTCGCAAGGA+AGG	0.507243	8_4:+7751388	None:intergenic
AAAGTTGTAGCACAAACAAT+GGG	0.536642	8_4:-7753170	Msa1311070:CDS
AAATATCATCTAGGACTAAC+AGG	0.400930	8_4:+7753282	None:intergenic
AACAAACTCCAAGATTTCTC+TGG	0.401149	8_4:+7753113	None:intergenic
AACACCTGTTAATAAGGAGG+AGG	0.587428	8_4:-7751137	Msa1311070:CDS
AACCAAACTCTTGAATCAAT+TGG	0.373359	8_4:-7753047	Msa1311070:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAAGTAAAACCTGTTAATG+AGG	+	chr8_4:7751125-7751144	None:intergenic	30.0%
AAAATCATGGTGTGCAAAAT+TGG	-	chr8_4:7751367-7751386	Msa1311070:CDS	30.0%
TTGAGAGTGATATTGTAAGA+AGG	-	chr8_4:7751640-7751659	Msa1311070:CDS	30.0%
TGAGAGTGATATTGTAAGAA+GGG	-	chr8_4:7751641-7751660	Msa1311070:CDS	30.0%
GTGTTAGAATCAGATATCAT+CGG	-	chr8_4:7751678-7751697	Msa1311070:CDS	30.0%
AACTATTGTGAAGAACATGT+TGG	-	chr8_4:7751899-7751918	Msa1311070:CDS	30.0%
AGTCGATGATAAGTTATCAT+TGG	-	chr8_4:7751941-7751960	Msa1311070:CDS	30.0%
TGTTAGTCCTAGATGATATT+TGG	-	chr8_4:7752012-7752031	Msa1311070:CDS	30.0%
TGTCATTCCAAATATCATCT+AGG	+	chr8_4:7752022-7752041	None:intergenic	30.0%
TAAAGTTGTAGCACAAACAA+TGG	-	chr8_4:7752121-7752140	Msa1311070:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	7751087	7754227	7751087	ID=Msa1311070;Name=Msa1311070
chr8_4	mRNA	7751087	7754227	7751087	ID=Msa1311070-mRNA-1;Parent=Msa1311070;Name=Msa1311070-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0\|0\|0\|1\|1\|1\|2\|0\|1010
chr8_4	exon	7754181	7754227	7754181	ID=Msa1311070-mRNA-1:exon:2870;Parent=Msa1311070-mRNA-1
chr8_4	exon	7751087	7754072	7751087	ID=Msa1311070-mRNA-1:exon:2869;Parent=Msa1311070-mRNA-1
chr8_4	CDS	7754181	7754227	7754181	ID=Msa1311070-mRNA-1:cds;Parent=Msa1311070-mRNA-1
chr8_4	CDS	7751087	7754072	7751087	ID=Msa1311070-mRNA-1:cds;Parent=Msa1311070-mRNA-1

Gene Sequence

>Msa1311070

ATGGCTGAACAAATCCCATATGCTGTTGCTGCTAGCCTCATTAACAGGTTGGCTTCTGCAGCCTTTCGTGAATTTGGACGAATTCATGGTGTTATGGATGAGCTGGAAAAGCTTAAGAATACTTTTGAATCGGTCAGAGCTGTGCTACTTGATGCCGAGGAGAAACAACACCAAAATCATGGTGTGCAAAATTGGATAAGAAGACTCAAAGATGATGTACTTCATCCTGCTGATGACTTGCTTGATGAATTTCTTATTGAAGATTTGCAACACAAACGAGATGAAGCTCATAAGAAGAAAGTGAGACAGGTACTTCATTCTTTATCTCCAAACAGAATTGCTTTTCGCCTTAAAATGGCTCACGAGATTGAAAAAATACAAAAAAAATTTAATGATGTGGTAAAAGATATGTCTGTGTTGAATCTAAACCCAAATGTTGTGGTGGTTGAGAGTGATATTGTAAGAAGGGAAACAAGTTCTTTTGTGTTAGAATCAGATATCATCGGAAGAGAAGATGATAAAAAGGAGATTTTAAGATTGTTGAGGCAATCACATGAAGATCAGCATGTCTCCGTGGTTGCTATTGTTGGGATTGGCGGTTTGGGAAAGACAGCTCTTGCTCAATTGGTATACAATGATGGTGAAGTAACGACGTCTTTTGAAAAGAGGATGTGGGTATGTGTCTCTGATAACTTTGATGTCAAAACTATTGTGAAGAACATGTTGGAGTCATTAACCAAAAAAAAAGTCGATGATAAGTTATCATTGGAAAACTTACAAAGCAAGCTACGTGACACCTTAACTGCTATGAGATACCTGTTAGTCCTAGATGATATTTGGAATGACAGTTTTGAAAAGTGGGCTCAATTAAAGACTTATATGATGTGTGGTGCTCAAGGTAGTAAGGTTGTAGTGACAACTCGCAGTAAAGTTGTAGCACAAACAATGGGTGTAAGTGTCCCCTATAGTTTGAATGGTTTGACTCCAGAGAAATCTTGGAGTTTGTTGAAGAACATCATTACAGATGGGGATGAGACCAAAGGAGTTTTGAACCAAACTCTTGAATCAATTGGTAAGAAGATAGCTGAAAAGTGCAGTGGTGTTCCACTAGCAATCAGAACACTAGGAGGCCTTTTACATGGTAAAAGTGAAGAAACGGAATGGGTTGGTGTTTTACAAGATGACTTCTGGAAATTATGTGAAGACGAAGAAAGTATCATGCCAGTGTTGAAACTGAGTTACCAAAACTTATCGCCTCAACTAAGACAATGTTTTTCTTACTGCTGTCTATATCCTAAGGATTGGGAAATAGAAAAGGATGAGTTGATTCAACTGTGGATGGCACAAGGTTATCTTGAATGTTCAACTCAAAAACAGTGTCTGGAAGATATTGGTAACCAATTCGTAAAAATTTTCTTGATGAAGTCATTCTTCCAAGATGCTAAAATTGATGATTGTGGTGATATATGTAGTTTCAAAATGCATGATTTAATACATGATCTTGCAATGCAAATAGCCGGCAATGATTGTTGTTACATAGGTAGTGAAACAAAAAGACTTGCAGGAAATCCCATTCATGTAATGTTGAAATTGGATTTTATTGGTTGGTTAGAGTCGTTGGATGCAAGCAGGTTGCGGACTTTGATTTTGTTGCATGGTGATTTGCCTAATCGTGGGTGTGACAAATTGTCTGTTATTTCAAAATTCAAACGCTTGCGTGTCTTGAGGGTGGAACCTTTATGTAACATTAAGTTGTGTGATTTCGTTGTAAAATTGAAGCATTTAAGATATCTTAAATTCTGGCAAATGAAAAGTCATGGAAGTGATATAAAAGCCTTCACTAATGCTGTTTGCCTACAAACACTAATATTGATGCTGAGCGACGAAAGATTTTCTCAAAACTTTGTCTCAAATTTAATCAATTTGAGACATCTTAATATTGTGGTTCTACCCCCTCCACCTCCCCTGCCAAAGGAGGCAAAATCTAGCAGATTTGGAGAACCGTGTGTGGGGAAGGTTCTTCCCATCTTTCGAGAACTGTGTGTGGGGAAGGTTCACAAGTGTGCAATTTTTTCCAAAGGGTATTCTTTACTCTCAAATATTGTTGAAATCTCTCTCACAGATTATAATGATTTGCCATATCTCCCACCAATGGAACGTCTACCATTTCTTAAATCACTTAAAATTCGCTATCTTCATAAATTGGAGTATATATTTTATGAAGAGCCTCTTTTGCGTGGATCATTCTTCCCATCTTTGGAGAAGCTAATTATTATTGGTTGTTCGGAACTGAGAGGATGGAGGAGGATGGGGGATGATGTCAATGATGATGATAACGCCTCACAATCTCATCATCTCTCTTTACCTTCTTTTTCTTCTCGTCTTTCACTAATAGAAATCACTGACTGTCTAATGTTGACATGCATGCCTACTTTTCCAAACCTTGATAAGAAATTGGAATTAGTAAGCTCTTGTGTGGAGCCATTAGAAGCAACATTTAACATGGTAGGTTCAAGATGTTTAATTGAATTCCCTCCTCTTTCCATGCTAAAACGCTTGATCTTTAAAGGGAAAAAATTGGATCTGAAAAAACTTCAAAAGGATTGGGTGCAAAATTTGACTTCTCTTGATCATATTGGTTTGTACTCGCTTCCAAATCAATCATTTCTGGAAATTGAAAATTGGTTTGTGGAAGACCCCAACTATCTCCCTTCCTTGCGAAGTATTACTTTCAGTGGAGCAGATTTCATGGCATTGCCAGATTGGATTTGCAAACTCTCCTCACTTCAGCATGTCAAAATTGAGTATTGTCGTGATTTGTCTTCGCTGCCCGAAGGAATGCCTTGCCTTTCCAAATTACAGACCCTAGAAATTATTAAATGTCGCTTCTTATTTGCAGAGTGTGAGACACAAACAAGTGCAACATGGCCCAAAATTTCTCACATTCCAAACATAATCCTAACACCTGTTAATAAGGAGGAGGAGGAGCAGGAGGACAAGTATGTACATGTCATCCTCTTAAACTACTGTTAA

Protein sequence

>Msa1311070

MAEQIPYAVAASLINRLASAAFREFGRIHGVMDELEKLKNTFESVRAVLLDAEEKQHQNHGVQNWIRRLKDDVLHPADDLLDEFLIEDLQHKRDEAHKKKVRQVLHSLSPNRIAFRLKMAHEIEKIQKKFNDVVKDMSVLNLNPNVVVVESDIVRRETSSFVLESDIIGREDDKKEILRLLRQSHEDQHVSVVAIVGIGGLGKTALAQLVYNDGEVTTSFEKRMWVCVSDNFDVKTIVKNMLESLTKKKVDDKLSLENLQSKLRDTLTAMRYLLVLDDIWNDSFEKWAQLKTYMMCGAQGSKVVVTTRSKVVAQTMGVSVPYSLNGLTPEKSWSLLKNIITDGDETKGVLNQTLESIGKKIAEKCSGVPLAIRTLGGLLHGKSEETEWVGVLQDDFWKLCEDEESIMPVLKLSYQNLSPQLRQCFSYCCLYPKDWEIEKDELIQLWMAQGYLECSTQKQCLEDIGNQFVKIFLMKSFFQDAKIDDCGDICSFKMHDLIHDLAMQIAGNDCCYIGSETKRLAGNPIHVMLKLDFIGWLESLDASRLRTLILLHGDLPNRGCDKLSVISKFKRLRVLRVEPLCNIKLCDFVVKLKHLRYLKFWQMKSHGSDIKAFTNAVCLQTLILMLSDERFSQNFVSNLINLRHLNIVVLPPPPPLPKEAKSSRFGEPCVGKVLPIFRELCVGKVHKCAIFSKGYSLLSNIVEISLTDYNDLPYLPPMERLPFLKSLKIRYLHKLEYIFYEEPLLRGSFFPSLEKLIIIGCSELRGWRRMGDDVNDDDNASQSHHLSLPSFSSRLSLIEITDCLMLTCMPTFPNLDKKLELVSSCVEPLEATFNMVGSRCLIEFPPLSMLKRLIFKGKKLDLKKLQKDWVQNLTSLDHIGLYSLPNQSFLEIENWFVEDPNYLPSLRSITFSGADFMALPDWICKLSSLQHVKIEYCRDLSSLPEGMPCLSKLQTLEIIKCRFLFAECETQTSATWPKISHIPNIILTPVNKEEEEQEDKYVHVILLNYC*