AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1441960


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1441960 MtrunA17_Chr8g0358741 99.517 207 1 0 1 207 46 252 4.35e-150 416
Msa1441960 MtrunA17_Chr3g0103341 37.500 192 113 3 23 207 41 232 5.18e-25 97.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1441960 AT3G12870.1 72.139 201 52 1 10 206 5 205 1.73e-99 287
Msa1441960 AT5G56120.1 38.000 200 117 3 15 207 36 235 3.25e-27 103

Find 51 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGCGGATTGAATCTATC+TGG 0.255908 tig0029550:+14183 None:intergenic
GTTTGTCTCTTGCTGTCATA+AGG 0.289653 tig0029550:-13770 Msa1441960:CDS
CATGATTTGTGACAAGCTTT+AGG 0.297093 tig0029550:+14077 None:intergenic
AAGGGTTATCTTGTGTAGTT+AGG 0.302967 tig0029550:-13751 None:intergenic
GAGTTTGTGGGTTGGTGTTT+CGG 0.319413 tig0029550:+14268 None:intergenic
CTTTGAGCCATTGCTTTAAG+AGG 0.341158 tig0029550:+14246 None:intergenic
GGGTTGGTGTTTCGGCAATT+TGG 0.381774 tig0029550:+14276 None:intergenic
GAGTTGAAAGTAAAGAAGTT+AGG 0.394819 tig0029550:-13842 Msa1441960:CDS
CTAAAGCTTGTCACAAATCA+TGG 0.398644 tig0029550:-14076 Msa1441960:CDS
TTTGTCTCTTGCTGTCATAA+GGG 0.402196 tig0029550:-13769 Msa1441960:CDS
GAGTGATGTAGAGGCTCATA+TGG 0.426429 tig0029550:-13994 Msa1441960:CDS
GGAGCATGAGGGTAGTTGAA+TGG 0.435925 tig0029550:+14126 None:intergenic
TAGGGCTTATATGGGCTTTG+AGG 0.443431 tig0029550:-14022 Msa1441960:CDS
AAGTTAGGAAATGGTCTTCA+AGG 0.444925 tig0029550:-13827 Msa1441960:CDS
GTTGGAGTTTGATTTGTTGA+AGG 0.447000 tig0029550:-13898 Msa1441960:CDS
TGAGGGTAGTTGAATGGAAG+AGG 0.447303 tig0029550:+14132 None:intergenic
AAAGTAAAGAAGTTAGGAAA+TGG 0.454954 tig0029550:-13836 Msa1441960:CDS
TTTGCGGATTGAATCTATCT+GGG 0.463339 tig0029550:+14184 None:intergenic
TTGCTTTAAGAGGAGTTTGT+GGG 0.465955 tig0029550:+14256 None:intergenic
AGGGTTATCTTGTGTAGTTA+GGG 0.471643 tig0029550:-13750 None:intergenic
AAAGCATTGTGATTTCTTTG+CGG 0.474720 tig0029550:+14168 None:intergenic
AAGAGTTGAAGAGGAGCATG+AGG 0.477929 tig0029550:+14114 None:intergenic
GTGGAGGAATTGAAGAAGAA+AGG 0.484418 tig0029550:-13921 Msa1441960:CDS
TCAGTGTTTGTATTAGCCAT+TGG 0.494026 tig0029550:+14356 None:intergenic
GATGCTTTCGAGGGAGAAAG+AGG 0.507945 tig0029550:-13967 Msa1441960:CDS
ATATGGAGAAGATGCTTTCG+AGG 0.512619 tig0029550:-13977 Msa1441960:CDS
CAGTGTAAGATCTTCAATCA+TGG 0.516363 tig0029550:-14324 Msa1441960:CDS
AAAGAAGAAGAACTCATCAA+TGG 0.532094 tig0029550:-14227 Msa1441960:CDS
GTTTCGGCAATTTGGTGAAG+TGG 0.538425 tig0029550:+14284 None:intergenic
GGAATTGAAGAAGAAAGGGT+TGG 0.541831 tig0029550:-13916 Msa1441960:CDS
TTTCGGCAATTTGGTGAAGT+GGG 0.543957 tig0029550:+14285 None:intergenic
ATTGCTTTAAGAGGAGTTTG+TGG 0.546009 tig0029550:+14255 None:intergenic
AGTTAGGAAATGGTCTTCAA+GGG 0.556873 tig0029550:-13826 Msa1441960:CDS
AATAACAAAGAACAGAGTGA+TGG 0.557124 tig0029550:+14050 None:intergenic
TAAGATCTTCAATCATGGAA+TGG 0.557240 tig0029550:-14319 Msa1441960:CDS
AGAGTTGAAGAGGAGCATGA+GGG 0.559380 tig0029550:+14115 None:intergenic
AGCTTTAGGTGTTGTATCAG+AGG 0.559678 tig0029550:+14091 None:intergenic
AAGAGGATCGCGGATTGTTG+AGG 0.566520 tig0029550:-13950 Msa1441960:CDS
AGGAAGTTGATGCTTTGAGA+AGG 0.575406 tig0029550:-13878 Msa1441960:CDS
TATCAGAGGAAGAGTTGAAG+AGG 0.581501 tig0029550:+14105 None:intergenic
GGAAGTTGATGCTTTGAGAA+GGG 0.582663 tig0029550:-13877 Msa1441960:CDS
ATAACAAAGAACAGAGTGAT+GGG 0.587025 tig0029550:+14051 None:intergenic
TGGAGGAATTGAAGAAGAAA+GGG 0.588646 tig0029550:-13920 Msa1441960:CDS
TTTAAGAGGAGTTTGTGGGT+TGG 0.603717 tig0029550:+14260 None:intergenic
GAGGTATAAGAGTGATGTAG+AGG 0.608995 tig0029550:-14003 Msa1441960:CDS
TAAAGCTTGTCACAAATCAT+GGG 0.609433 tig0029550:-14075 Msa1441960:CDS
ACAAACTCCTCTTAAAGCAA+TGG 0.614010 tig0029550:-14253 Msa1441960:CDS
TATGGAGAAGATGCTTTCGA+GGG 0.614054 tig0029550:-13976 Msa1441960:CDS
CGGATTGTTGAGGAAATGTG+TGG 0.678295 tig0029550:-13940 Msa1441960:CDS
TCGAGGGAGAAAGAGGATCG+CGG 0.687511 tig0029550:-13960 Msa1441960:CDS
ATTGTTGAGGAAATGTGTGG+AGG 0.718156 tig0029550:-13937 Msa1441960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTCTTTGTTATTTTCACT+AGG - tig0029550:14064-14083 Msa1441960:CDS 25.0%
!!! GTTCTTTGTTATTTTCACTA+GGG - tig0029550:14065-14084 Msa1441960:CDS 25.0%
! AAAGTAAAGAAGTTAGGAAA+TGG - tig0029550:14269-14288 Msa1441960:CDS 25.0%
TAAGATCTTCAATCATGGAA+TGG - tig0029550:13786-13805 Msa1441960:CDS 30.0%
AAAGAAGAAGAACTCATCAA+TGG - tig0029550:13878-13897 Msa1441960:CDS 30.0%
!! AAAGCATTGTGATTTCTTTG+CGG + tig0029550:13940-13959 None:intergenic 30.0%
TAAAGCTTGTCACAAATCAT+GGG - tig0029550:14030-14049 Msa1441960:CDS 30.0%
ATAACAAAGAACAGAGTGAT+GGG + tig0029550:14057-14076 None:intergenic 30.0%
AATAACAAAGAACAGAGTGA+TGG + tig0029550:14058-14077 None:intergenic 30.0%
! TATTTTCACTAGGGCTTATA+TGG - tig0029550:14074-14093 Msa1441960:CDS 30.0%
! ATTTTCACTAGGGCTTATAT+GGG - tig0029550:14075-14094 Msa1441960:CDS 30.0%
GAGTTGAAAGTAAAGAAGTT+AGG - tig0029550:14263-14282 Msa1441960:CDS 30.0%
!! TTCTCTGTTTTTCTTCTCAA+TGG - tig0029550:14309-14328 Msa1441960:CDS 30.0%
CAGTGTAAGATCTTCAATCA+TGG - tig0029550:13781-13800 Msa1441960:CDS 35.0%
!! TTGCTTTAAGAGGAGTTTGT+GGG + tig0029550:13852-13871 None:intergenic 35.0%
!! ATTGCTTTAAGAGGAGTTTG+TGG + tig0029550:13853-13872 None:intergenic 35.0%
ACAAACTCCTCTTAAAGCAA+TGG - tig0029550:13852-13871 Msa1441960:CDS 35.0%
! TTTGCGGATTGAATCTATCT+GGG + tig0029550:13924-13943 None:intergenic 35.0%
! CATGATTTGTGACAAGCTTT+AGG + tig0029550:14031-14050 None:intergenic 35.0%
CTAAAGCTTGTCACAAATCA+TGG - tig0029550:14029-14048 Msa1441960:CDS 35.0%
TGGAGGAATTGAAGAAGAAA+GGG - tig0029550:14185-14204 Msa1441960:CDS 35.0%
!! GTTGGAGTTTGATTTGTTGA+AGG - tig0029550:14207-14226 Msa1441960:CDS 35.0%
AAGTTAGGAAATGGTCTTCA+AGG - tig0029550:14278-14297 Msa1441960:CDS 35.0%
AGTTAGGAAATGGTCTTCAA+GGG - tig0029550:14279-14298 Msa1441960:CDS 35.0%
! TTTGTCTCTTGCTGTCATAA+GGG - tig0029550:14336-14355 Msa1441960:CDS 35.0%
! TTTCGGCAATTTGGTGAAGT+GGG + tig0029550:13823-13842 None:intergenic 40.0%
TTTAAGAGGAGTTTGTGGGT+TGG + tig0029550:13848-13867 None:intergenic 40.0%
! CTTTGAGCCATTGCTTTAAG+AGG + tig0029550:13862-13881 None:intergenic 40.0%
! CTTTGCGGATTGAATCTATC+TGG + tig0029550:13925-13944 None:intergenic 40.0%
TATCAGAGGAAGAGTTGAAG+AGG + tig0029550:14003-14022 None:intergenic 40.0%
!! AGCTTTAGGTGTTGTATCAG+AGG + tig0029550:14017-14036 None:intergenic 40.0%
GAGGTATAAGAGTGATGTAG+AGG - tig0029550:14102-14121 Msa1441960:CDS 40.0%
ATATGGAGAAGATGCTTTCG+AGG - tig0029550:14128-14147 Msa1441960:CDS 40.0%
TATGGAGAAGATGCTTTCGA+GGG - tig0029550:14129-14148 Msa1441960:CDS 40.0%
ATTGTTGAGGAAATGTGTGG+AGG - tig0029550:14168-14187 Msa1441960:CDS 40.0%
GTGGAGGAATTGAAGAAGAA+AGG - tig0029550:14184-14203 Msa1441960:CDS 40.0%
GGAATTGAAGAAGAAAGGGT+TGG - tig0029550:14189-14208 Msa1441960:CDS 40.0%
!! AGGAAGTTGATGCTTTGAGA+AGG - tig0029550:14227-14246 Msa1441960:CDS 40.0%
!! GGAAGTTGATGCTTTGAGAA+GGG - tig0029550:14228-14247 Msa1441960:CDS 40.0%
! GTTTGTCTCTTGCTGTCATA+AGG - tig0029550:14335-14354 Msa1441960:CDS 40.0%
! GTTTCGGCAATTTGGTGAAG+TGG + tig0029550:13824-13843 None:intergenic 45.0%
!! GAGTTTGTGGGTTGGTGTTT+CGG + tig0029550:13840-13859 None:intergenic 45.0%
TGAGGGTAGTTGAATGGAAG+AGG + tig0029550:13976-13995 None:intergenic 45.0%
! AGAGTTGAAGAGGAGCATGA+GGG + tig0029550:13993-14012 None:intergenic 45.0%
! AAGAGTTGAAGAGGAGCATG+AGG + tig0029550:13994-14013 None:intergenic 45.0%
TAGGGCTTATATGGGCTTTG+AGG - tig0029550:14083-14102 Msa1441960:CDS 45.0%
GAGTGATGTAGAGGCTCATA+TGG - tig0029550:14111-14130 Msa1441960:CDS 45.0%
CGGATTGTTGAGGAAATGTG+TGG - tig0029550:14165-14184 Msa1441960:CDS 45.0%
!! GGGTTGGTGTTTCGGCAATT+TGG + tig0029550:13832-13851 None:intergenic 50.0%
GGAGCATGAGGGTAGTTGAA+TGG + tig0029550:13982-14001 None:intergenic 50.0%
GATGCTTTCGAGGGAGAAAG+AGG - tig0029550:14138-14157 Msa1441960:CDS 50.0%
! AAGAGGATCGCGGATTGTTG+AGG - tig0029550:14155-14174 Msa1441960:CDS 50.0%
TCGAGGGAGAAAGAGGATCG+CGG - tig0029550:14145-14164 Msa1441960:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0029550 gene 13752 14375 13752 ID=Msa1441960;Name=Msa1441960
tig0029550 mRNA 13752 14375 13752 ID=Msa1441960-mRNA-1;Parent=Msa1441960;Name=Msa1441960-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|207
tig0029550 exon 13752 14375 13752 ID=Msa1441960-mRNA-1:exon:13117;Parent=Msa1441960-mRNA-1
tig0029550 CDS 13752 14375 13752 ID=Msa1441960-mRNA-1:cds;Parent=Msa1441960-mRNA-1
Gene Sequence

>Msa1441960

ATGGCTAATACAAACACTGATGAACACAACAGTGTAAGATCTTCAATCATGGAATGGAAACAAATGCACCCACTTCACCAAATTGCCGAAACACCAACCCACAAACTCCTCTTAAAGCAATGGCTCAAAGAAGAAGAACTCATCAATGGTCGCATAGCTTTAAAAGAAACCCAGATAGATTCAATCCGCAAAGAAATCACAATGCTTTACATCTTCTTCTTCCTCTTCCATTCAACTACCCTCATGCTCCTCTTCAACTCTTCCTCTGATACAACACCTAAAGCTTGTCACAAATCATGGGTCCCATCACTCTGTTCTTTGTTATTTTCACTAGGGCTTATATGGGCTTTGAGGTATAAGAGTGATGTAGAGGCTCATATGGAGAAGATGCTTTCGAGGGAGAAAGAGGATCGCGGATTGTTGAGGAAATGTGTGGAGGAATTGAAGAAGAAAGGGTTGGAGTTTGATTTGTTGAAGGAAGTTGATGCTTTGAGAAGGGCTAAGAGTTTGAGAGTTGAAAGTAAAGAAGTTAGGAAATGGTCTTCAAGGGATTTTGTTTCTCTGTTTTTCTTCTCAATGGCTTGTTTGTCTCTTGCTGTCATAAGGGTTATCTTGTGTAGTTAG

Protein sequence

>Msa1441960

MANTNTDEHNSVRSSIMEWKQMHPLHQIAETPTHKLLLKQWLKEEELINGRIALKETQIDSIRKEITMLYIFFFLFHSTTLMLLFNSSSDTTPKACHKSWVPSLCSLLFSLGLIWALRYKSDVEAHMEKMLSREKEDRGLLRKCVEELKKKGLEFDLLKEVDALRRAKSLRVESKEVRKWSSRDFVSLFFFSMACLSLAVIRVILCS*