AlfalfaGEDB | Msa1447100

M. sativa cultivar zhongmu-4 / Msa1447100

KEGG: K07024
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0008150 GO:0008374 GO:0009987 GO:0012505 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071554
PFAM: PF14416.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1447100	sp\|Q940H3\|TBL36_ARATH	61.741	379	131	6	2	376	4	372	3.90e-172	488

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1447100	A0A072VSC4	95.288	382	14	1	1	378	1	382	0.0	765

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000370	Msa1447100	0.805871	1.083039e-49	-8.615850e-47
Msa0013270	Msa1447100	0.814080	1.848435e-51	-8.615850e-47
Msa0019900	Msa1447100	0.804511	2.085549e-49	-8.615850e-47
Msa0025160	Msa1447100	0.840937	6.434076e-58	-8.615850e-47
Msa0029380	Msa1447100	0.807201	5.674300e-50	-8.615850e-47
Msa0033220	Msa1447100	0.807524	4.845588e-50	-8.615850e-47
Msa0044160	Msa1447100	0.981018	1.403903e-151	-8.615850e-47
Msa0045810	Msa1447100	0.833445	5.310308e-56	-8.615850e-47
Msa0046200	Msa1447100	0.811536	6.667543e-51	-8.615850e-47
Msa0062190	Msa1447100	0.819967	8.795187e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1447100	MtrunA17_Chr1g0210771	95.288	382	14	1	1	378	1	382	0.0	765
Msa1447100	MtrunA17_Chr1g0171171	73.615	379	95	3	1	378	1	375	0.0	603
Msa1447100	MtrunA17_Chr3g0105301	42.216	334	175	5	55	374	43	372	8.30e-99	298
Msa1447100	MtrunA17_Chr5g0430371	40.233	343	181	5	53	374	56	395	2.20e-98	298
Msa1447100	MtrunA17_Chr2g0283581	42.598	331	169	6	55	378	42	358	5.02e-96	290
Msa1447100	MtrunA17_Chr4g0006991	42.075	347	177	8	53	378	84	427	1.78e-92	283
Msa1447100	MtrunA17_Chr2g0325481	39.759	332	179	6	54	378	49	366	4.00e-92	281
Msa1447100	MtrunA17_Chr3g0097891	42.216	334	169	7	55	378	31	350	4.71e-92	280
Msa1447100	MtrunA17_Chr7g0258851	39.211	380	207	8	19	378	212	587	4.01e-90	283
Msa1447100	MtrunA17_Chr2g0314791	42.687	335	167	8	53	378	27	345	2.97e-89	273

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1447100	AT3G54260.1	61.741	379	131	6	2	376	4	372	3.96e-173	488
Msa1447100	AT5G58600.1	40.533	338	184	6	55	378	66	400	4.34e-93	285
Msa1447100	AT2G31110.2	38.736	364	197	7	21	374	11	358	3.13e-92	281
Msa1447100	AT1G29050.1	41.390	331	174	5	55	378	61	378	5.35e-92	281
Msa1447100	AT2G34070.1	40.118	339	180	7	49	378	59	383	6.18e-90	276
Msa1447100	AT2G30900.1	40.841	333	174	8	53	374	41	361	2.22e-89	274
Msa1447100	AT5G01360.2	38.329	347	191	8	53	378	34	378	6.47e-86	265
Msa1447100	AT3G62390.1	38.872	337	186	6	53	374	136	467	6.87e-86	268
Msa1447100	AT5G06700.1	38.251	366	201	7	37	378	230	594	1.72e-85	271
Msa1447100	AT5G01360.1	38.329	347	191	8	53	378	90	434	2.89e-85	266

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Find 97 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTGGGTGGATTTGAATT+TGG	0.291594	tig0031598:+2183	Msa1447100:CDS
AAATATCAGGCACACCAGGG+AGG	0.749580	tig0031598:-2598	None:intergenic
AAATGGAAGTGGAAGCCATT+AGG	0.444002	tig0031598:+1302	Msa1447100:CDS
AAGAAATGTGATTTCTCAGT+TGG	0.430768	tig0031598:+1173	Msa1447100:CDS
AAGGAGTTATTCCAACTGAC+AGG	0.516555	tig0031598:+1668	Msa1447100:CDS
AAGTATCTTGGTAAAATGAG+AGG	0.596417	tig0031598:+1577	Msa1447100:CDS
AATGGCTGCAATCAGAGGAC+TGG	0.587580	tig0031598:-2571	None:intergenic
ACATCCTTCAGTGTATAGTA+AGG	0.542161	tig0031598:+2511	Msa1447100:CDS
ACTCAATACATGGGCAAAGT+GGG	0.582566	tig0031598:+2168	Msa1447100:CDS
AGAAACTGAAGACGTCAACA+TGG	0.659763	tig0031598:+1127	Msa1447100:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTCTTCCATGAATCTCTTT+GGG	-	tig0031598:1159-1178	None:intergenic	30.0%
ATTTCTTCCATGAATCTCTT+TGG	-	tig0031598:1160-1179	None:intergenic	30.0%
AAGAAATGTGATTTCTCAGT+TGG	+	tig0031598:1173-1192	Msa1447100:CDS	30.0%
CATTTCTCATAATCTGAATC+CGG	-	tig0031598:1287-1306	None:intergenic	30.0%
TAATGTTTCATCATCACCTT+GGG	-	tig0031598:1337-1356	None:intergenic	30.0%
TTAATGTTTCATCATCACCT+TGG	-	tig0031598:1338-1357	None:intergenic	30.0%
AGAAGCTAAATTTATCAGCA+TGG	-	tig0031598:1529-1548	None:intergenic	30.0%
AAGTATCTTGGTAAAATGAG+AGG	+	tig0031598:1577-1596	Msa1447100:CDS	30.0%
ATAGCATGGAAAGACATTAA+AGG	-	tig0031598:1715-1734	None:intergenic	30.0%
TTTAATGTCTTTCCATGCTA+TGG	+	tig0031598:1714-1733	Msa1447100:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0031598	gene	1017	2812	1017	ID=Msa1447100;Name=Msa1447100
tig0031598	mRNA	1017	2812	1017	ID=Msa1447100-mRNA-1;Parent=Msa1447100;Name=Msa1447100-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0\|1\|0.8\|1\|1\|1\|5\|163\|378
tig0031598	exon	1017	1339	1017	ID=Msa1447100-mRNA-1:exon:15741;Parent=Msa1447100-mRNA-1
tig0031598	exon	1564	1735	1564	ID=Msa1447100-mRNA-1:exon:15742;Parent=Msa1447100-mRNA-1
tig0031598	exon	1813	1994	1813	ID=Msa1447100-mRNA-1:exon:15743;Parent=Msa1447100-mRNA-1
tig0031598	exon	2099	2257	2099	ID=Msa1447100-mRNA-1:exon:15744;Parent=Msa1447100-mRNA-1
tig0031598	exon	2349	2812	2349	ID=Msa1447100-mRNA-1:exon:15745;Parent=Msa1447100-mRNA-1
tig0031598	CDS	1017	1339	1017	ID=Msa1447100-mRNA-1:cds;Parent=Msa1447100-mRNA-1
tig0031598	CDS	1564	1735	1564	ID=Msa1447100-mRNA-1:cds;Parent=Msa1447100-mRNA-1
tig0031598	CDS	1813	1994	1813	ID=Msa1447100-mRNA-1:cds;Parent=Msa1447100-mRNA-1
tig0031598	CDS	2099	2257	2099	ID=Msa1447100-mRNA-1:cds;Parent=Msa1447100-mRNA-1
tig0031598	CDS	2349	2649	2349	ID=Msa1447100-mRNA-1:cds;Parent=Msa1447100-mRNA-1
tig0031598	three_prime_UTR	2650	2812	2650	ID=Msa1447100-mRNA-1:three_prime_utr;Parent=Msa1447100-mRNA-1

Gene Sequence

>Msa1447100

ATGGCAAAAACAAACCTTCATTTACTGTCATTTTTATTCTTCTTAGGCTTATTCAACTGCACATTATCACTGATCAATCCAGAGGAAGTGTCCAGTTGGCTTGCATTGGAAGAAACTGAAGACGTCAACATGGTGCAAACCCAAAGAGATTCATGGAAGAAATGTGATTTCTCAGTTGGAAAATGGATATTTGATCAAACCTACCCTCTCTATGATTCCAACTGTCCTTATCTCGCCACTGCAGTAACTTGTCAAAAGAATGGACGGCCGGATTCAGATTATGAGAAATGGAAGTGGAAGCCATTAGGTTGTTCCATCCCAAGGTTTGATGCACTGAAGTATCTTGGTAAAATGAGAGGAAAGAGAATGATGTTGGTGGGCGATTCTATAATGAGAAACCAATGGGAATCTCTTGTATGCTTAGTTCAAGGAGTTATTCCAACTGACAGGAAAAAGGTCACCTATGATGGTCCTTTAATGTCTTTCCATGCTATGGATTTTGAGACATCAATTGAGTTCTTCTGGGCACCACTATTGGTAGAACTGAAGAAAGGGTCTAATAATAAGAGAATTCTACATTTGGATTTGATTGAAGAGAATGCAAGGTATTGGAAAGGAGTTGATATTCTTGCATTTGATTCAGCTCATTGGTGGACTCATTCTGGTCAATCTAGCTCGTGGGATTATTACATGGAAGGAAATAGTATCATCACAAACATGAATCAAATGGTTGCCTATGAGAAAGGACTCAATACATGGGCAAAGTGGGTGGATTTGAATTTGGATCCTCGAAAAACCCGAGTCGTTTTTAGAAGTATGGCACCTAGACATAACAGGCAAAATGGTTGGAAATGTTACAATCAAAGGCAACCTGTAAAATATTTCAGCCACATGCATGTTCCAGGACCAGTAGTAGTGCTAAAAGGAGTGCTGAAGAGAATGAGATTTCCAGTATATCTACAGGATATTACAACAATGACTGCATTTAGAAGAGATGGACATCCTTCAGTGTATAGTAAGGTAATAAGTGAAGAAGAGAGGCAGAAACAAGGTAATGGCCAGTCCTCTGATTGCAGCCATTGGTGCCTCCCTGGTGTGCCTGATATTTGGAACGAAATGCTGAGTGCATGGCTTTAA

Protein sequence

>Msa1447100

MAKTNLHLLSFLFFLGLFNCTLSLINPEEVSSWLALEETEDVNMVQTQRDSWKKCDFSVGKWIFDQTYPLYDSNCPYLATAVTCQKNGRPDSDYEKWKWKPLGCSIPRFDALKYLGKMRGKRMMLVGDSIMRNQWESLVCLVQGVIPTDRKKVTYDGPLMSFHAMDFETSIEFFWAPLLVELKKGSNNKRILHLDLIEENARYWKGVDILAFDSAHWWTHSGQSSSWDYYMEGNSIITNMNQMVAYEKGLNTWAKWVDLNLDPRKTRVVFRSMAPRHNRQNGWKCYNQRQPVKYFSHMHVPGPVVVLKGVLKRMRFPVYLQDITTMTAFRRDGHPSVYSKVISEEERQKQGNGQSSDCSHWCLPGVPDIWNEMLSAWL*