AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1364040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1364040 MtrunA17_Chr2g0330051 94.660 412 15 2 1 411 1 406 0.0 684
Msa1364040 MtrunA17_Chr8g0342551 53.273 443 161 14 1 408 1 432 4.80e-108 325
Msa1364040 MtrunA17_Chr3g0135851 46.380 442 185 13 1 400 1 432 1.82e-84 265
Msa1364040 MtrunA17_Chr4g0025441 41.328 271 136 6 1 258 1 261 1.02e-37 141
Msa1364040 MtrunA17_Chr6g0462191 35.974 303 155 7 81 354 105 397 4.18e-29 117
Msa1364040 MtrunA17_Chr8g0346021 41.451 193 85 7 81 258 125 304 9.81e-29 118
Msa1364040 MtrunA17_Chr1g0200161 37.989 179 99 5 82 257 140 309 3.50e-27 112
Msa1364040 MtrunA17_Chr1g0181581 39.896 193 98 7 60 250 99 275 9.65e-27 111
Msa1364040 MtrunA17_Chr7g0263731 31.287 342 164 10 57 347 88 409 1.32e-25 108
Msa1364040 MtrunA17_Chr1g0204901 46.087 115 49 2 82 183 135 249 1.45e-23 102
Msa1364040 MtrunA17_Chr4g0020671 38.060 134 74 3 82 209 100 230 2.23e-21 95.5
Msa1364040 MtrunA17_Chr2g0316401 34.659 176 99 4 85 257 76 238 2.69e-21 94.0
Msa1364040 MtrunA17_Chr2g0283321 34.270 178 105 3 85 262 65 230 6.36e-21 92.4
Msa1364040 MtrunA17_Chr4g0060381 35.593 177 91 4 1 157 1 174 3.85e-20 92.8
Msa1364040 MtrunA17_Chr8g0340761 33.854 192 84 4 1 149 1 192 7.19e-20 90.9
Msa1364040 MtrunA17_Chr4g0057321 36.486 148 89 1 1 143 1 148 4.61e-19 88.6
Msa1364040 MtrunA17_Chr1g0166431 33.772 228 101 6 1 181 7 231 6.47e-19 88.2
Msa1364040 MtrunA17_Chr3g0126901 31.206 282 144 9 83 347 112 360 2.62e-18 86.7
Msa1364040 MtrunA17_Chr8g0371131 36.424 151 74 2 1 146 1 134 3.01e-18 80.9
Msa1364040 MtrunA17_Chr1g0146931 39.583 192 97 5 81 253 120 311 4.93e-18 85.9
Msa1364040 MtrunA17_Chr4g0045641 31.724 145 83 2 79 211 142 282 4.90e-17 83.2
Msa1364040 MtrunA17_Chr2g0330921 34.043 188 100 6 1 164 1 188 6.31e-17 81.6
Msa1364040 MtrunA17_Chr8g0389781 57.576 66 28 0 82 147 125 190 6.40e-17 82.4
Msa1364040 MtrunA17_Chr6g0472551 62.903 62 23 0 85 146 119 180 7.53e-17 82.0
Msa1364040 MtrunA17_Chr1g0172611 26.403 303 170 10 85 374 107 369 2.36e-16 80.1
Msa1364040 MtrunA17_Chr7g0273951 30.055 183 87 4 1 151 1 174 2.56e-16 80.1
Msa1364040 MtrunA17_Chr1g0203711 56.061 66 29 0 78 143 110 175 3.97e-16 79.7
Msa1364040 MtrunA17_Chr7g0273861 50.000 66 33 0 82 147 124 189 2.37e-15 77.4
Msa1364040 MtrunA17_Chr5g0416161 38.614 101 62 0 61 161 97 197 3.23e-13 71.6
Msa1364040 MtrunA17_Chr1g0156121 50.746 67 33 0 83 149 124 190 6.20e-13 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1364040 AT1G72670.1 46.698 424 166 18 1 393 1 395 2.72e-88 274
Msa1364040 AT2G26180.1 48.201 417 173 12 1 390 1 401 6.23e-86 268
Msa1364040 AT1G17480.1 42.033 364 161 12 1 344 1 334 9.52e-63 206
Msa1364040 AT5G03040.1 39.904 208 115 5 49 254 80 279 1.09e-32 128
Msa1364040 AT5G03040.3 39.904 208 115 5 49 254 80 279 1.09e-32 128
Msa1364040 AT5G03040.2 39.904 208 115 5 49 254 80 279 1.09e-32 128
Msa1364040 AT3G22190.3 38.929 280 124 8 1 258 1 255 7.08e-32 125
Msa1364040 AT3G22190.2 38.929 280 124 8 1 258 1 255 7.08e-32 125
Msa1364040 AT3G22190.4 38.929 280 124 8 1 258 1 255 7.08e-32 125
Msa1364040 AT3G22190.1 38.929 280 124 8 1 258 1 255 7.08e-32 125
Msa1364040 AT1G01110.2 33.333 246 139 6 34 265 74 308 4.07e-27 113
Msa1364040 AT4G00820.1 38.095 189 89 5 81 253 130 306 4.49e-26 110
Msa1364040 AT3G52290.1 37.572 173 97 4 82 253 107 269 6.73e-25 106
Msa1364040 AT3G09710.1 37.017 181 99 6 81 258 106 274 2.09e-24 105
Msa1364040 AT3G49260.3 35.519 183 97 5 85 253 122 297 3.30e-24 104
Msa1364040 AT3G49260.1 35.519 183 96 6 85 253 122 296 4.66e-24 104
Msa1364040 AT3G49260.4 35.519 183 96 6 85 253 122 296 4.66e-24 104
Msa1364040 AT3G49260.2 35.519 183 96 6 85 253 122 296 4.66e-24 104
Msa1364040 AT4G10640.1 29.504 383 187 13 7 347 18 359 5.74e-24 103
Msa1364040 AT3G09710.2 34.896 192 102 5 81 258 106 288 6.60e-24 103
Msa1364040 AT4G14750.1 28.169 284 158 9 1 251 1 271 1.53e-22 99.0
Msa1364040 AT4G14750.3 28.169 284 158 9 1 251 60 330 2.32e-22 99.0
Msa1364040 AT4G14750.2 29.371 286 152 10 1 251 60 330 2.62e-22 99.0
Msa1364040 AT3G59690.2 37.324 142 75 3 74 211 161 292 9.42e-21 94.7
Msa1364040 AT3G59690.1 37.324 142 75 3 74 211 161 292 9.42e-21 94.7
Msa1364040 AT3G15050.1 31.429 210 119 5 85 285 52 245 1.63e-20 90.9
Msa1364040 AT2G26410.2 34.054 185 103 5 81 258 146 318 6.95e-20 92.0
Msa1364040 AT2G26410.1 34.054 185 103 5 81 258 146 318 6.95e-20 92.0
Msa1364040 AT1G18840.1 33.500 200 101 6 1 169 1 199 6.84e-19 89.4
Msa1364040 AT1G18840.3 33.500 200 101 6 1 169 1 199 6.84e-19 89.4
Msa1364040 AT1G18840.2 33.500 200 101 6 1 169 1 199 6.84e-19 89.4
Msa1364040 AT2G33990.1 31.818 176 109 3 82 257 46 210 7.79e-19 86.3
Msa1364040 AT4G23060.1 58.108 74 31 0 83 156 167 240 5.56e-18 86.3
Msa1364040 AT4G29150.1 42.500 120 61 2 71 182 119 238 1.50e-17 84.3
Msa1364040 AT5G13460.1 36.552 145 71 4 77 209 103 238 5.75e-17 82.8
Msa1364040 AT1G51960.1 50.000 80 37 2 71 149 89 166 7.52e-17 81.6
Msa1364040 AT3G51380.1 57.576 66 28 0 81 146 35 100 1.00e-16 75.9
Msa1364040 AT3G16490.1 44.565 92 49 1 82 171 106 197 1.11e-16 81.6
Msa1364040 AT5G07240.1 28.819 288 147 9 82 365 104 337 3.13e-16 80.5
Msa1364040 AT5G07240.2 28.819 288 147 9 82 365 150 383 4.98e-16 80.1
Msa1364040 AT1G14380.1 34.146 164 89 4 1 149 1 160 1.26e-15 79.3
Msa1364040 AT1G14380.3 34.146 164 89 4 1 149 1 160 1.26e-15 79.3
Msa1364040 AT1G14380.4 34.146 164 89 4 1 149 1 160 1.26e-15 79.3
Msa1364040 AT1G14380.6 34.146 164 89 4 1 149 1 160 1.41e-15 79.0
Msa1364040 AT1G14380.5 34.146 164 89 4 1 149 1 160 1.41e-15 79.0
Msa1364040 AT1G14380.2 34.146 164 89 4 1 149 1 160 1.41e-15 79.0
Msa1364040 AT1G14380.7 34.146 164 89 4 1 149 1 160 1.79e-15 79.0
Msa1364040 AT1G74690.1 51.429 70 34 0 80 149 110 179 2.19e-15 78.6
Msa1364040 AT5G03960.3 38.060 134 80 2 85 215 110 243 2.19e-14 74.7
Msa1364040 AT5G03960.2 38.060 134 80 2 85 215 110 243 2.19e-14 74.7
Msa1364040 AT5G03960.1 38.060 134 80 2 85 215 109 242 2.28e-14 74.7
Msa1364040 AT2G43680.1 33.099 142 88 4 68 209 311 445 2.42e-14 75.5
Msa1364040 AT2G43680.4 33.099 142 88 4 68 209 310 444 2.44e-14 75.5
Msa1364040 AT2G43680.2 33.099 142 88 4 68 209 310 444 2.44e-14 75.5
Msa1364040 AT2G43680.3 33.099 142 88 4 68 209 310 444 2.44e-14 75.5
Msa1364040 AT2G43680.5 33.099 142 88 4 68 209 310 444 2.44e-14 75.5
Msa1364040 AT1G19870.1 35.338 133 68 3 61 177 177 307 1.15e-13 73.6
Msa1364040 AT1G19870.2 38.938 113 56 2 76 177 14 124 1.22e-13 73.2
Msa1364040 AT3G49380.1 36.134 119 66 2 27 139 52 166 2.00e-13 71.6
Msa1364040 AT5G62070.1 64.151 53 19 0 85 137 119 171 5.89e-13 70.5

Find 95 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGACCCTTCAGTTAATTTA+TGG 0.180664 tig0018106:+70599 Msa1364040:CDS
CAATGGGTGTTTCTGGAAAA+TGG 0.187083 tig0018106:+68350 Msa1364040:exon
GTTAGCCACTTTCTCATAAA+TGG 0.214297 tig0018106:-70835 None:intergenic
GTAGGGTTGTGTTGTGAAAT+TGG 0.229055 tig0018106:-68299 None:intergenic
TAGGGTTGTGTTGTGAAATT+GGG 0.233355 tig0018106:-68298 None:intergenic
GGCTCTCTTTCGAGCCTTCT+TGG 0.249229 tig0018106:+68705 Msa1364040:CDS
GCTATGTCTGTGGGGATTAC+AGG 0.271977 tig0018106:-70979 None:intergenic
TGATAATAACTTGCCCTTAG+AGG 0.285753 tig0018106:-70640 None:intergenic
CTAAAGATTTCATCCTTATC+AGG 0.290356 tig0018106:+68656 Msa1364040:CDS
TCACTCTTCCTGCTTGAGAT+TGG 0.302121 tig0018106:-70223 None:intergenic
TTAACTTCATCTGCAGTTCC+AGG 0.344133 tig0018106:-69911 None:intergenic
GCAAGCTCTGGTTAGGGTTC+AGG 0.355190 tig0018106:+69646 Msa1364040:CDS
ATGGGCTGCTATTCGCATTC+AGG 0.358781 tig0018106:+68684 Msa1364040:CDS
AACAAGCAGCTGTTACTCTA+AGG 0.370485 tig0018106:+69618 Msa1364040:CDS
CTGATAAGGATGAAATCTTT+AGG 0.373735 tig0018106:-68655 None:intergenic
CACAGTGAGATGAACTGATT+TGG 0.379942 tig0018106:+70762 Msa1364040:three_prime_UTR
GTAATAGTACTAAGAAGAAA+TGG 0.387683 tig0018106:+68503 Msa1364040:CDS
AGGCTAAGCTCAAAACTTAC+AGG 0.390491 tig0018106:+70473 Msa1364040:CDS
GGTGTATGCAAGCTCTGGTT+AGG 0.390529 tig0018106:+69639 Msa1364040:CDS
TTATCAACTTCATGTACATC+TGG 0.395939 tig0018106:+70373 Msa1364040:CDS
ATCGTTGTGCAACACCTCTA+AGG 0.396953 tig0018106:+70626 Msa1364040:CDS
CAAGGATGGTGTGAAATTCC+TGG 0.403362 tig0018106:+69893 Msa1364040:CDS
GATAATAGTATAAACAGTTC+TGG 0.410910 tig0018106:+70574 Msa1364040:CDS
TTCCTTCTTGATTGAAAAGT+AGG 0.412386 tig0018106:-70256 None:intergenic
CATTGCTCTATCCCTCTTGA+TGG 0.414300 tig0018106:-69963 None:intergenic
TTCCTAAGCTCTTGTTGTCA+TGG 0.417603 tig0018106:-70150 None:intergenic
AGTTATTATCAAAATCGATA+TGG 0.428695 tig0018106:+70652 Msa1364040:CDS
CAGCCCATTCCTGCCTGATA+AGG 0.431812 tig0018106:-68669 None:intergenic
CATCCTTATCAGGCAGGAAT+GGG 0.450099 tig0018106:+68666 Msa1364040:CDS
GTTGCTGTCGTTGTTCGAGC+TGG 0.450476 tig0018106:+68631 Msa1364040:CDS
AAGAGCTTTGAGAGCATTGA+GGG 0.454446 tig0018106:+69550 Msa1364040:CDS
CTAAGAAGAAATGGAAGCTA+TGG 0.454548 tig0018106:+68512 Msa1364040:CDS
AGGGTTGTGTTGTGAAATTG+GGG 0.473403 tig0018106:-68297 None:intergenic
TCATCCTTATCAGGCAGGAA+TGG 0.473411 tig0018106:+68665 Msa1364040:CDS
AATCCCCACAGACATAGCTT+TGG 0.475015 tig0018106:+70984 Msa1364040:three_prime_UTR
TTGAAATTTGAAAGTGTAGG+AGG 0.478393 tig0018106:-68227 None:intergenic
TTCCAATAAGAAAACCGATC+AGG 0.485410 tig0018106:+68387 Msa1364040:CDS
AGACTGCAAGCAATATTTAG+AGG 0.495590 tig0018106:+69581 Msa1364040:CDS
GAAATAGCTTGTTAGAGCGC+TGG 0.496586 tig0018106:+70170 Msa1364040:CDS
TCTTCAACAATGAAGAAGAC+TGG 0.507403 tig0018106:+68556 Msa1364040:CDS
TGCAAGCAATATTTAGAGGC+AGG 0.511357 tig0018106:+69585 Msa1364040:CDS
TCTAAGGTGTATGCAAGCTC+TGG 0.513287 tig0018106:+69634 Msa1364040:CDS
GAAGGAATGGTGTGACAACT+AGG 0.517271 tig0018106:+70272 Msa1364040:CDS
AGATTTCATCCTTATCAGGC+AGG 0.519223 tig0018106:+68660 Msa1364040:CDS
ATCATCTTCGACTTTAAGCT+CGG 0.520897 tig0018106:+70327 Msa1364040:CDS
GTGTATGCAAGCTCTGGTTA+GGG 0.521748 tig0018106:+69640 Msa1364040:CDS
GTTATTATTATCAGCAAGGA+TGG 0.522365 tig0018106:+69879 Msa1364040:intron
GGCAAGAAGGTGCCATCAAG+AGG 0.522656 tig0018106:+69951 Msa1364040:CDS
TGATTGTAAATTTACCTGAT+CGG 0.522844 tig0018106:-68401 None:intergenic
TGGCAAACAAACCATGTCCA+TGG 0.529332 tig0018106:+70194 Msa1364040:CDS
GGCAGCAGCAGTATCGGTAA+TGG 0.530108 tig0018106:-68608 None:intergenic
CCCTTCAGTTAATTTATGGA+AGG 0.533849 tig0018106:+70603 Msa1364040:CDS
TTCAGGCACGTGTGAGAGCT+AGG 0.537833 tig0018106:+69663 Msa1364040:CDS
AACCATGACAACAAGAGCTT+AGG 0.537945 tig0018106:+70148 Msa1364040:CDS
AGCAACGGCAGCAGCAGTAT+CGG 0.538131 tig0018106:-68614 None:intergenic
AAGCTAAGAATGAGGCAAGA+AGG 0.538231 tig0018106:+69938 Msa1364040:CDS
TCAAATTCATGTAGCTAGGT+AGG 0.539947 tig0018106:-70438 None:intergenic
GAGCTTAAAGTCGAAGATGA+TGG 0.542387 tig0018106:-70325 None:intergenic
TCCTTCCATAAATTAACTGA+AGG 0.545088 tig0018106:-70604 None:intergenic
CGGTGCCATTTATGAGAAAG+TGG 0.546625 tig0018106:+70830 Msa1364040:three_prime_UTR
ATTGGACTCTCCCATGGACA+TGG 0.548204 tig0018106:-70205 None:intergenic
CAGAATCAGCATGGTTGCGA+TGG 0.549893 tig0018106:-69736 None:intergenic
TAATCCAAAGCTATGTCTGT+GGG 0.555077 tig0018106:-70988 None:intergenic
ATTATCAAAATCGATATGGC+AGG 0.555854 tig0018106:+70656 Msa1364040:CDS
TCGTTGTGCAACACCTCTAA+GGG 0.556401 tig0018106:+70627 Msa1364040:CDS
CTTGAGATTGGACTCTCCCA+TGG 0.558321 tig0018106:-70211 None:intergenic
GGTTGTGTTGTGAAATTGGG+GGG 0.560503 tig0018106:-68295 None:intergenic
AAAAGAAATAGTTACCAAGA+AGG 0.563052 tig0018106:-68719 None:intergenic
GAGTTCGAAGAGACTCGTAA+TGG 0.563998 tig0018106:+70507 Msa1364040:CDS
TTTGAGAGCATTGAGGGCAG+TGG 0.564489 tig0018106:+69556 Msa1364040:CDS
GAAGAGCTTTGAGAGCATTG+AGG 0.569321 tig0018106:+69549 Msa1364040:CDS
CCTTCCATAAATTAACTGAA+GGG 0.587260 tig0018106:-70603 None:intergenic
TCTGTCAAATTCATGTAGCT+AGG 0.592487 tig0018106:-70442 None:intergenic
GAATTTGACAGAATCAACTA+AGG 0.594832 tig0018106:+70453 Msa1364040:CDS
ATCTATGCCAGAAGCAACAG+AGG 0.595241 tig0018106:+70402 Msa1364040:CDS
GGGAGAGTCCAATCTCAAGC+AGG 0.595864 tig0018106:+70215 Msa1364040:CDS
GGCAAACAAACCATGTCCAT+GGG 0.598464 tig0018106:+70195 Msa1364040:CDS
TTAAAGCAAAGCTAAGAATG+AGG 0.599503 tig0018106:+69930 Msa1364040:CDS
TGATTCTGTTAAGCTTGTAG+AGG 0.611493 tig0018106:+69751 Msa1364040:CDS
AGCTCGAACAACGACAGCAA+CGG 0.615911 tig0018106:-68629 None:intergenic
TTAATCCAAAGCTATGTCTG+TGG 0.618385 tig0018106:-70989 None:intergenic
CATCAAGAGGGATAGAGCAA+TGG 0.627816 tig0018106:+69964 Msa1364040:CDS
TAGCTTGTTAGAGCGCTGGA+TGG 0.632949 tig0018106:+70174 Msa1364040:CDS
TCAACACTTAATTACCTGTG+TGG 0.634403 tig0018106:-69999 None:intergenic
AATTTGAAATTTGAAAGTGT+AGG 0.634771 tig0018106:-68230 None:intergenic
AAATTTGAAAGTGTAGGAGG+TGG 0.636927 tig0018106:-68224 None:intergenic
GCAAGAAGGTGCCATCAAGA+GGG 0.646053 tig0018106:+69952 Msa1364040:CDS
GTGTGAGAGCTAGGAATGTG+AGG 0.652883 tig0018106:+69672 Msa1364040:CDS
GGGTTGTGTTGTGAAATTGG+GGG 0.654292 tig0018106:-68296 None:intergenic
CAAGCTTAACAGAATCAGCA+TGG 0.655634 tig0018106:-69745 None:intergenic
GGCATACTCTCTATCCACAC+AGG 0.672618 tig0018106:+69985 Msa1364040:CDS
AATGTGAGGAAATCTCCAGA+AGG 0.676143 tig0018106:+69686 Msa1364040:CDS
AATCCAAAGCTATGTCTGTG+GGG 0.708556 tig0018106:-70987 None:intergenic
CTTTCACTCAATCAATACAG+CGG 0.736905 tig0018106:+70810 Msa1364040:three_prime_UTR
TCACTGTGTCAAGATGACAA+TGG 0.742872 tig0018106:-70747 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTCAAAATATGATAATATA+AGG - tig0018106:69154-69173 None:intergenic 10.0%
!! TAAAACAACAACAAAAAAAA+GGG - tig0018106:68444-68463 None:intergenic 15.0%
!! TTAAAACAACAACAAAAAAA+AGG - tig0018106:68445-68464 None:intergenic 15.0%
!!! TTAATTAGTTTATCTTATGA+TGG + tig0018106:69128-69147 Msa1364040:intron 15.0%
!! TAAAAAGTTGAATTTCAATT+AGG - tig0018106:69205-69224 None:intergenic 15.0%
!! AAAGAGAAAGAAAAAATAAA+GGG - tig0018106:69257-69276 None:intergenic 15.0%
!! AAAAGAGAAAGAAAAAATAA+AGG - tig0018106:69258-69277 None:intergenic 15.0%
!!! TTATTTTTTCTTTCTCTTTT+TGG + tig0018106:69258-69277 Msa1364040:intron 15.0%
!!! TGTATTTTAAAAAGAAATAG+AGG - tig0018106:69519-69538 None:intergenic 15.0%
!!! TTCTTTTTAAAATACATTGA+AGG + tig0018106:69523-69542 Msa1364040:intron 15.0%
!! ATTACATAATATTCTCTAAA+TGG + tig0018106:69802-69821 Msa1364040:intron 15.0%
!!! AATTTGAAATTTGAAAGTGT+AGG - tig0018106:68233-68252 None:intergenic 20.0%
!! AAGAGAAAGAAAAAATAAAG+GGG - tig0018106:69256-69275 None:intergenic 20.0%
!! TGCAATAAATCCTAAAAAAA+TGG - tig0018106:69400-69419 None:intergenic 20.0%
!! TTATAGTATATGCTAACATA+AGG + tig0018106:69843-69862 Msa1364040:intron 20.0%
!! TATAGTATATGCTAACATAA+GGG + tig0018106:69844-69863 Msa1364040:intron 20.0%
!! AGTTATTATCAAAATCGATA+TGG + tig0018106:70652-70671 Msa1364040:CDS 20.0%
!!! TAAATTTTGAGTAAGAAGTA+CGG + tig0018106:71038-71057 Msa1364040:three_prime_UTR 20.0%
! TGATTGTAAATTTACCTGAT+CGG - tig0018106:68404-68423 None:intergenic 25.0%
! GTAATAGTACTAAGAAGAAA+TGG + tig0018106:68503-68522 Msa1364040:CDS 25.0%
! AAAAGAAATAGTTACCAAGA+AGG - tig0018106:68722-68741 None:intergenic 25.0%
! AAATCATTCAACCCAAATAA+AGG - tig0018106:68949-68968 None:intergenic 25.0%
!! ATTTGCTAGTGTTTATAACT+CGG - tig0018106:68989-69008 None:intergenic 25.0%
! GTTGAATTTCAATTAGGTAA+AGG - tig0018106:69199-69218 None:intergenic 25.0%
!! TTTTCTTTCTCTTTTTGGAT+TGG + tig0018106:69263-69282 Msa1364040:intron 25.0%
!! TTTCTTTCTCTTTTTGGATT+GGG + tig0018106:69264-69283 Msa1364040:intron 25.0%
!!! TAAACACTGTCCATTTTTTT+AGG + tig0018106:69387-69406 Msa1364040:intron 25.0%
! AAAAAAAAAGTAACCAAGAG+TGG - tig0018106:69433-69452 None:intergenic 25.0%
!!! AATGGTTATTTTGAGTTACA+AGG + tig0018106:69820-69839 Msa1364040:intron 25.0%
! AAAAGCAAGCAAAGATATAT+TGG - tig0018106:70056-70075 None:intergenic 25.0%
! GATAATAGTATAAACAGTTC+TGG + tig0018106:70574-70593 Msa1364040:CDS 25.0%
!! GTAGATAAAAATTTTACGTG+AGG + tig0018106:70865-70884 Msa1364040:three_prime_UTR 25.0%
! TTGAAATTTGAAAGTGTAGG+AGG - tig0018106:68230-68249 None:intergenic 30.0%
! AGTGTTAATGTTGTTTGTTC+AGG + tig0018106:68471-68490 Msa1364040:intron 30.0%
CTGATAAGGATGAAATCTTT+AGG - tig0018106:68658-68677 None:intergenic 30.0%
CTAAAGATTTCATCCTTATC+AGG + tig0018106:68656-68675 Msa1364040:CDS 30.0%
TGAAATTCCAAGACCAATTA+TGG + tig0018106:68797-68816 Msa1364040:intron 30.0%
CAATTATGGATCAGAATTCA+GGG + tig0018106:68811-68830 Msa1364040:intron 30.0%
TTACAAGATCTACCAATAGT+TGG + tig0018106:68853-68872 Msa1364040:intron 30.0%
TACAAGATCTACCAATAGTT+GGG + tig0018106:68854-68873 Msa1364040:intron 30.0%
GGATTTGGGAGTTTAAAAAA+CGG + tig0018106:68875-68894 Msa1364040:intron 30.0%
GACTTAACATGAAAACACAA+AGG - tig0018106:68902-68921 None:intergenic 30.0%
!!! TCCTCCTTTTTTCCTTTATT+TGG + tig0018106:68934-68953 Msa1364040:intron 30.0%
!!! CCTCCTTTTTTCCTTTATTT+GGG + tig0018106:68935-68954 Msa1364040:intron 30.0%
CAACCCAAATAAAGGAAAAA+AGG - tig0018106:68941-68960 None:intergenic 30.0%
GTTGAATGATTTACGTAGTT+AGG + tig0018106:68957-68976 Msa1364040:intron 30.0%
TAAAACAATGTGAAGAAGAG+TGG - tig0018106:69038-69057 None:intergenic 30.0%
! ATTTTACAGTCATCTTAAGG+AGG - tig0018106:69230-69249 None:intergenic 30.0%
! AGGATTTTACAGTCATCTTA+AGG - tig0018106:69233-69252 None:intergenic 30.0%
AGAAAGAAAAAATAAAGGGG+AGG - tig0018106:69253-69272 None:intergenic 30.0%
! TTCTTTCTCTTTTTGGATTG+GGG + tig0018106:69265-69284 Msa1364040:intron 30.0%
CCTTGCTGATAATAATAACA+TGG - tig0018106:69878-69897 None:intergenic 30.0%
CCATGTTATTATTATCAGCA+AGG + tig0018106:69875-69894 Msa1364040:intron 30.0%
GTTATTATTATCAGCAAGGA+TGG + tig0018106:69879-69898 Msa1364040:intron 30.0%
TTAAAGCAAAGCTAAGAATG+AGG + tig0018106:69930-69949 Msa1364040:CDS 30.0%
!!! GATTTTGTGGATTTTGAGTT+TGG - tig0018106:70103-70122 None:intergenic 30.0%
!! TTCCTACTTTTCAATCAAGA+AGG + tig0018106:70254-70273 Msa1364040:CDS 30.0%
TTCCTTCTTGATTGAAAAGT+AGG - tig0018106:70259-70278 None:intergenic 30.0%
! ACTTTTCAATCAAGAAGGAA+TGG + tig0018106:70259-70278 Msa1364040:CDS 30.0%
TTATCAACTTCATGTACATC+TGG + tig0018106:70373-70392 Msa1364040:CDS 30.0%
GAATTTGACAGAATCAACTA+AGG + tig0018106:70453-70472 Msa1364040:CDS 30.0%
! ACAAGTACAACTTTTCTGAA+TGG + tig0018106:70550-70569 Msa1364040:CDS 30.0%
CCTTCCATAAATTAACTGAA+GGG - tig0018106:70606-70625 None:intergenic 30.0%
TCCTTCCATAAATTAACTGA+AGG - tig0018106:70607-70626 None:intergenic 30.0%
ATTATCAAAATCGATATGGC+AGG + tig0018106:70656-70675 Msa1364040:CDS 30.0%
! AAATTTGAAAGTGTAGGAGG+TGG - tig0018106:68227-68246 None:intergenic 35.0%
TAGGGTTGTGTTGTGAAATT+GGG - tig0018106:68301-68320 None:intergenic 35.0%
! CGATTTTTGCAGAGATTGTA+GGG - tig0018106:68319-68338 None:intergenic 35.0%
CCTACAATCTCTGCAAAAAT+CGG + tig0018106:68317-68336 Msa1364040:five_prime_UTR 35.0%
!! AAATCGGTTCTTTTCTCCAA+TGG + tig0018106:68333-68352 Msa1364040:exon 35.0%
!! AATCGGTTCTTTTCTCCAAT+GGG + tig0018106:68334-68353 Msa1364040:exon 35.0%
TTCCAATAAGAAAACCGATC+AGG + tig0018106:68387-68406 Msa1364040:CDS 35.0%
!! TACCTGATCGGTTTTCTTAT+TGG - tig0018106:68392-68411 None:intergenic 35.0%
CTAAGAAGAAATGGAAGCTA+TGG + tig0018106:68512-68531 Msa1364040:CDS 35.0%
TCTTCAACAATGAAGAAGAC+TGG + tig0018106:68556-68575 Msa1364040:CDS 35.0%
CCACAGCAAAAATCATAGAA+AGG - tig0018106:68745-68764 None:intergenic 35.0%
! CCTTTCTATGATTTTTGCTG+TGG + tig0018106:68742-68761 Msa1364040:intron 35.0%
! TTCTGATCCATAATTGGTCT+TGG - tig0018106:68807-68826 None:intergenic 35.0%
CCTGAATTCTGATCCATAAT+TGG - tig0018106:68813-68832 None:intergenic 35.0%
CCAATTATGGATCAGAATTC+AGG + tig0018106:68810-68829 Msa1364040:intron 35.0%
ATCTACCAATAGTTGGGATT+TGG + tig0018106:68860-68879 Msa1364040:intron 35.0%
TCTACCAATAGTTGGGATTT+GGG + tig0018106:68861-68880 Msa1364040:intron 35.0%
CCCAAATAAAGGAAAAAAGG+AGG - tig0018106:68938-68957 None:intergenic 35.0%
!! TCTTTCTCTTTTTGGATTGG+GGG + tig0018106:69266-69285 Msa1364040:intron 35.0%
! AGTAGGTAGGTTCTTTTGTA+TGG + tig0018106:69305-69324 Msa1364040:intron 35.0%
! TAGGTTCTTTTGTATGGTTG+TGG + tig0018106:69311-69330 Msa1364040:intron 35.0%
AGACTGCAAGCAATATTTAG+AGG + tig0018106:69581-69600 Msa1364040:CDS 35.0%
TGATTCTGTTAAGCTTGTAG+AGG + tig0018106:69751-69770 Msa1364040:CDS 35.0%
TCAACACTTAATTACCTGTG+TGG - tig0018106:70002-70021 None:intergenic 35.0%
!!! GAAAAGAGACACTGATTTTG+TGG - tig0018106:70116-70135 None:intergenic 35.0%
ATCATCTTCGACTTTAAGCT+CGG + tig0018106:70327-70346 Msa1364040:CDS 35.0%
TCAAATTCATGTAGCTAGGT+AGG - tig0018106:70441-70460 None:intergenic 35.0%
TCTGTCAAATTCATGTAGCT+AGG - tig0018106:70445-70464 None:intergenic 35.0%
CTGACCCTTCAGTTAATTTA+TGG + tig0018106:70599-70618 Msa1364040:CDS 35.0%
CCCTTCAGTTAATTTATGGA+AGG + tig0018106:70603-70622 Msa1364040:CDS 35.0%
! TGATAATAACTTGCCCTTAG+AGG - tig0018106:70643-70662 None:intergenic 35.0%
CTTTCACTCAATCAATACAG+CGG + tig0018106:70810-70829 Msa1364040:three_prime_UTR 35.0%
! GTTAGCCACTTTCTCATAAA+TGG - tig0018106:70838-70857 None:intergenic 35.0%
TAATCCAAAGCTATGTCTGT+GGG - tig0018106:70991-71010 None:intergenic 35.0%
TTAATCCAAAGCTATGTCTG+TGG - tig0018106:70992-71011 None:intergenic 35.0%
AGGGTTGTGTTGTGAAATTG+GGG - tig0018106:68300-68319 None:intergenic 40.0%
GTAGGGTTGTGTTGTGAAAT+TGG - tig0018106:68302-68321 None:intergenic 40.0%
! CCGATTTTTGCAGAGATTGT+AGG - tig0018106:68320-68339 None:intergenic 40.0%
! TTTTCTCCAATGGGTGTTTC+TGG + tig0018106:68343-68362 Msa1364040:exon 40.0%
! CATTTTCCAGAAACACCCAT+TGG - tig0018106:68352-68371 None:intergenic 40.0%
!! CAATGGGTGTTTCTGGAAAA+TGG + tig0018106:68350-68369 Msa1364040:exon 40.0%
AGATTTCATCCTTATCAGGC+AGG + tig0018106:68660-68679 Msa1364040:CDS 40.0%
TGGATCAGAATTCAGGGATT+AGG + tig0018106:68817-68836 Msa1364040:intron 40.0%
AACTCCCAAATCCCAACTAT+TGG - tig0018106:68868-68887 None:intergenic 40.0%
!! AAGAGCTTTGAGAGCATTGA+GGG + tig0018106:69550-69569 Msa1364040:CDS 40.0%
TGCAAGCAATATTTAGAGGC+AGG + tig0018106:69585-69604 Msa1364040:CDS 40.0%
AACAAGCAGCTGTTACTCTA+AGG + tig0018106:69618-69637 Msa1364040:CDS 40.0%
AATGTGAGGAAATCTCCAGA+AGG + tig0018106:69686-69705 Msa1364040:CDS 40.0%
CAAGCTTAACAGAATCAGCA+TGG - tig0018106:69748-69767 None:intergenic 40.0%
TTAACTTCATCTGCAGTTCC+AGG - tig0018106:69914-69933 None:intergenic 40.0%
AAGCTAAGAATGAGGCAAGA+AGG + tig0018106:69938-69957 Msa1364040:CDS 40.0%
AACCATGACAACAAGAGCTT+AGG + tig0018106:70148-70167 Msa1364040:CDS 40.0%
! TTCCTAAGCTCTTGTTGTCA+TGG - tig0018106:70153-70172 None:intergenic 40.0%
GAGCTTAAAGTCGAAGATGA+TGG - tig0018106:70328-70347 None:intergenic 40.0%
!! TCTTTTTCCTCTGTTGCTTC+TGG - tig0018106:70412-70431 None:intergenic 40.0%
AGGCTAAGCTCAAAACTTAC+AGG + tig0018106:70473-70492 Msa1364040:CDS 40.0%
TCACTGTGTCAAGATGACAA+TGG - tig0018106:70750-70769 None:intergenic 40.0%
CACAGTGAGATGAACTGATT+TGG + tig0018106:70762-70781 Msa1364040:three_prime_UTR 40.0%
AATCCAAAGCTATGTCTGTG+GGG - tig0018106:70990-71009 None:intergenic 40.0%
GGTTGTGTTGTGAAATTGGG+GGG - tig0018106:68298-68317 None:intergenic 45.0%
GGGTTGTGTTGTGAAATTGG+GGG - tig0018106:68299-68318 None:intergenic 45.0%
TCATCCTTATCAGGCAGGAA+TGG + tig0018106:68665-68684 Msa1364040:CDS 45.0%
CATCCTTATCAGGCAGGAAT+GGG + tig0018106:68666-68685 Msa1364040:CDS 45.0%
!! TTCTCTTTTTGGATTGGGGG+AGG + tig0018106:69269-69288 Msa1364040:intron 45.0%
!! TCTCTTTTTGGATTGGGGGA+GGG + tig0018106:69270-69289 Msa1364040:intron 45.0%
GGTCAATGACTTCAGTAGGT+AGG + tig0018106:69292-69311 Msa1364040:intron 45.0%
!! TGTGGTGCTACTAATCTGTC+AGG + tig0018106:69329-69348 Msa1364040:intron 45.0%
!! GTGGTGCTACTAATCTGTCA+GGG + tig0018106:69330-69349 Msa1364040:intron 45.0%
!! TGGTGCTACTAATCTGTCAG+GGG + tig0018106:69331-69350 Msa1364040:intron 45.0%
GCAGAAATATCAGCCACTCT+TGG + tig0018106:69417-69436 Msa1364040:intron 45.0%
!! GAAGAGCTTTGAGAGCATTG+AGG + tig0018106:69549-69568 Msa1364040:CDS 45.0%
! TCTAAGGTGTATGCAAGCTC+TGG + tig0018106:69634-69653 Msa1364040:CDS 45.0%
! GTGTATGCAAGCTCTGGTTA+GGG + tig0018106:69640-69659 Msa1364040:CDS 45.0%
CAAGGATGGTGTGAAATTCC+TGG + tig0018106:69893-69912 Msa1364040:CDS 45.0%
CATTGCTCTATCCCTCTTGA+TGG - tig0018106:69966-69985 None:intergenic 45.0%
CATCAAGAGGGATAGAGCAA+TGG + tig0018106:69964-69983 Msa1364040:CDS 45.0%
!! GAAATAGCTTGTTAGAGCGC+TGG + tig0018106:70170-70189 Msa1364040:CDS 45.0%
TGGCAAACAAACCATGTCCA+TGG + tig0018106:70194-70213 Msa1364040:CDS 45.0%
GGCAAACAAACCATGTCCAT+GGG + tig0018106:70195-70214 Msa1364040:CDS 45.0%
TCACTCTTCCTGCTTGAGAT+TGG - tig0018106:70226-70245 None:intergenic 45.0%
GAAGGAATGGTGTGACAACT+AGG + tig0018106:70272-70291 Msa1364040:CDS 45.0%
ATCTATGCCAGAAGCAACAG+AGG + tig0018106:70402-70421 Msa1364040:CDS 45.0%
GAGTTCGAAGAGACTCGTAA+TGG + tig0018106:70507-70526 Msa1364040:CDS 45.0%
ATCGTTGTGCAACACCTCTA+AGG + tig0018106:70626-70645 Msa1364040:CDS 45.0%
TCGTTGTGCAACACCTCTAA+GGG + tig0018106:70627-70646 Msa1364040:CDS 45.0%
!! CGGTGCCATTTATGAGAAAG+TGG + tig0018106:70830-70849 Msa1364040:three_prime_UTR 45.0%
AATCCCCACAGACATAGCTT+TGG + tig0018106:70984-71003 Msa1364040:three_prime_UTR 45.0%
AGCTCGAACAACGACAGCAA+CGG - tig0018106:68632-68651 None:intergenic 50.0%
! ATGGGCTGCTATTCGCATTC+AGG + tig0018106:68684-68703 Msa1364040:CDS 50.0%
!! CTCTTTTTGGATTGGGGGAG+GGG + tig0018106:69271-69290 Msa1364040:intron 50.0%
GAGGGGTCAATGACTTCAGT+AGG + tig0018106:69288-69307 Msa1364040:intron 50.0%
!! TTTGAGAGCATTGAGGGCAG+TGG + tig0018106:69556-69575 Msa1364040:CDS 50.0%
! GGTGTATGCAAGCTCTGGTT+AGG + tig0018106:69639-69658 Msa1364040:CDS 50.0%
GTGTGAGAGCTAGGAATGTG+AGG + tig0018106:69672-69691 Msa1364040:CDS 50.0%
!! TGCTGCACTGCTTTTCCTTC+TGG - tig0018106:69704-69723 None:intergenic 50.0%
CAGAATCAGCATGGTTGCGA+TGG - tig0018106:69739-69758 None:intergenic 50.0%
!! GCAAGAAGGTGCCATCAAGA+GGG + tig0018106:69952-69971 Msa1364040:CDS 50.0%
GGCATACTCTCTATCCACAC+AGG + tig0018106:69985-70004 Msa1364040:CDS 50.0%
!! TAGCTTGTTAGAGCGCTGGA+TGG + tig0018106:70174-70193 Msa1364040:CDS 50.0%
ATTGGACTCTCCCATGGACA+TGG - tig0018106:70208-70227 None:intergenic 50.0%
CTTGAGATTGGACTCTCCCA+TGG - tig0018106:70214-70233 None:intergenic 50.0%
GCTATGTCTGTGGGGATTAC+AGG - tig0018106:70982-71001 None:intergenic 50.0%
!! GGCAGCAGCAGTATCGGTAA+TGG - tig0018106:68611-68630 None:intergenic 55.0%
AGCAACGGCAGCAGCAGTAT+CGG - tig0018106:68617-68636 None:intergenic 55.0%
GTTGCTGTCGTTGTTCGAGC+TGG + tig0018106:68631-68650 Msa1364040:CDS 55.0%
CAGCCCATTCCTGCCTGATA+AGG - tig0018106:68672-68691 None:intergenic 55.0%
GGCTCTCTTTCGAGCCTTCT+TGG + tig0018106:68705-68724 Msa1364040:CDS 55.0%
! GCAAGCTCTGGTTAGGGTTC+AGG + tig0018106:69646-69665 Msa1364040:CDS 55.0%
TTCAGGCACGTGTGAGAGCT+AGG + tig0018106:69663-69682 Msa1364040:CDS 55.0%
!! GGCAAGAAGGTGCCATCAAG+AGG + tig0018106:69951-69970 Msa1364040:CDS 55.0%
GGGAGAGTCCAATCTCAAGC+AGG + tig0018106:70215-70234 Msa1364040:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0018106 gene 68219 71061 68219 ID=Msa1364040;Name=Msa1364040
tig0018106 mRNA 68219 71061 68219 ID=Msa1364040-mRNA-1;Parent=Msa1364040;Name=Msa1364040-mRNA-1;_AED=0.07;_eAED=0.07;_QI=133|1|1|1|1|1|5|377|411
tig0018106 exon 68219 68408 68219 ID=Msa1364040-mRNA-1:exon:13776;Parent=Msa1364040-mRNA-1
tig0018106 exon 68493 68726 68493 ID=Msa1364040-mRNA-1:exon:13777;Parent=Msa1364040-mRNA-1
tig0018106 exon 69545 69772 69545 ID=Msa1364040-mRNA-1:exon:13778;Parent=Msa1364040-mRNA-1
tig0018106 exon 69893 70006 69893 ID=Msa1364040-mRNA-1:exon:13779;Parent=Msa1364040-mRNA-1
tig0018106 exon 70082 71061 70082 ID=Msa1364040-mRNA-1:exon:13780;Parent=Msa1364040-mRNA-1
tig0018106 five_prime_UTR 68219 68351 68219 ID=Msa1364040-mRNA-1:five_prime_utr;Parent=Msa1364040-mRNA-1
tig0018106 CDS 68352 68408 68352 ID=Msa1364040-mRNA-1:cds;Parent=Msa1364040-mRNA-1
tig0018106 CDS 68493 68726 68493 ID=Msa1364040-mRNA-1:cds;Parent=Msa1364040-mRNA-1
tig0018106 CDS 69545 69772 69545 ID=Msa1364040-mRNA-1:cds;Parent=Msa1364040-mRNA-1
tig0018106 CDS 69893 70006 69893 ID=Msa1364040-mRNA-1:cds;Parent=Msa1364040-mRNA-1
tig0018106 CDS 70082 70684 70082 ID=Msa1364040-mRNA-1:cds;Parent=Msa1364040-mRNA-1
tig0018106 three_prime_UTR 70685 71061 70685 ID=Msa1364040-mRNA-1:three_prime_utr;Parent=Msa1364040-mRNA-1
Gene Sequence

>Msa1364040

ATGGGTGTTTCTGGAAAATGGTTTAAATCACTTCTTTCCAATAAGAAAACCGATCAGGAGAAGAAGTGTAATAGTACTAAGAAGAAATGGAAGCTATGGAAAACTTCTTCTTCTTCTTCTTCTTCAACAATGAAGAAGACTGGTGCTGCTGCTGCTGCTGTGTCTGATTCTTCCATTACCGATACTGCTGCTGCCGTTGCTGTCGTTGTTCGAGCTGGACCTAAAGATTTCATCCTTATCAGGCAGGAATGGGCTGCTATTCGCATTCAGGCTCTCTTTCGAGCCTTCTTGGCTAGAAGAGCTTTGAGAGCATTGAGGGCAGTGGTGAGACTGCAAGCAATATTTAGAGGCAGGCAAGTGAGAAAACAAGCAGCTGTTACTCTAAGGTGTATGCAAGCTCTGGTTAGGGTTCAGGCACGTGTGAGAGCTAGGAATGTGAGGAAATCTCCAGAAGGAAAAGCAGTGCAGCAGCTATTAGATGACCATCGCAACCATGCTGATTCTGTTAAGCTTGTAGAGCAAGGATGGTGTGAAATTCCTGGAACTGCAGATGAAGTTAAAGCAAAGCTAAGAATGAGGCAAGAAGGTGCCATCAAGAGGGATAGAGCAATGGCATACTCTCTATCCACACAGTCAAGAATAAGTGCTAGTCCAAACTCAAAATCCACAAAATCAGTGTCTCTTTTCAAGCATCATCACAACCATGACAACAAGAGCTTAGGAAATAGCTTGTTAGAGCGCTGGATGGCAAACAAACCATGTCCATGGGAGAGTCCAATCTCAAGCAGGAAGAGTGAAGAACTTGTTCCTACTTTTCAATCAAGAAGGAATGGTGTGACAACTAGGATTTCAGCTCTTAAAATCTGTCAGCAAACTCCATCATCTTCGACTTTAAGCTCGGAATACATGAATGATGATAGCATTTTATCAACTTCATGTACATCTGGATTAACATCTATGCCAGAAGCAACAGAGGAAAAAGATGCTCACCTACCTAGCTACATGAATTTGACAGAATCAACTAAGGCTAAGCTCAAAACTTACAGGTCTTCTTCGCAGAGTTCGAAGAGACTCGTAATGGATGATTGTATGTCTCATAACACAAGTACAACTTTTCTGAATGGAGATAATAGTATAAACAGTTCTGGTTCTGACCCTTCAGTTAATTTATGGAAGGATCGTTGTGCAACACCTCTAAGGGCAAGTTATTATCAAAATCGATATGGCAGGAGATAG
Protein sequence

>Msa1364040

MGVSGKWFKSLLSNKKTDQEKKCNSTKKKWKLWKTSSSSSSSTMKKTGAAAAAVSDSSITDTAAAVAVVVRAGPKDFILIRQEWAAIRIQALFRAFLARRALRALRAVVRLQAIFRGRQVRKQAAVTLRCMQALVRVQARVRARNVRKSPEGKAVQQLLDDHRNHADSVKLVEQGWCEIPGTADEVKAKLRMRQEGAIKRDRAMAYSLSTQSRISASPNSKSTKSVSLFKHHHNHDNKSLGNSLLERWMANKPCPWESPISSRKSEELVPTFQSRRNGVTTRISALKICQQTPSSSTLSSEYMNDDSILSTSCTSGLTSMPEATEEKDAHLPSYMNLTESTKAKLKTYRSSSQSSKRLVMDDCMSHNTSTTFLNGDNSINSSGSDPSVNLWKDRCATPLRASYYQNRYGRR*