AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene063327


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063327.t1 MTR_6g090520 93.182 132 4 1 1 132 35 161 3.11e-86 248
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063327.t1 AT1G68590 84.444 90 14 0 43 132 76 165 3.25e-55 170
MS.gene063327.t1 AT1G68590 82.222 90 16 0 43 132 73 162 2.61e-54 168
MS.gene063327.t1 AT5G15760 70.588 102 30 0 30 131 82 183 3.07e-50 158

Find 36 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTAAGCCCTTATTACTTT+TGG 0.215786 6.1:-78369993 MS.gene063327:CDS
TTCCTTGGCCAAAAGTAATA+AGG 0.216981 6.1:+78369985 None:intergenic
GACTCGTGTTGAAGTTCATT+TGG 0.338552 6.1:-78370074 MS.gene063327:CDS
TCCTTGGCCAAAAGTAATAA+GGG 0.369106 6.1:+78369986 None:intergenic
CAAAAGTAATAAGGGCTTAG+AGG 0.406041 6.1:+78369994 None:intergenic
TCTTCCCAAGCATCTTTCCT+TGG 0.419427 6.1:+78369970 None:intergenic
CCACTGATATTATCAATTTG+TGG 0.436998 6.1:-78369879 MS.gene063327:CDS
CGTGTTGAAGTTCATTTGGA+TGG 0.444833 6.1:-78370070 MS.gene063327:CDS
TGAGGTGGAGGTTGAAGAGA+AGG 0.448959 6.1:+78371487 None:intergenic
ATGGAGAAGAACATCGGTAT+TGG 0.450928 6.1:-78370051 MS.gene063327:CDS
ACGAGTCTTGGTTTGTCTGT+TGG 0.451057 6.1:+78370090 None:intergenic
TCAACCTCCACCTCAATCTC+AGG 0.453824 6.1:-78371479 MS.gene063327:intron
ATGAACTTCAACACGAGTCT+TGG 0.461392 6.1:+78370078 None:intergenic
TTGGCCAAGGAAAGATGCTT+GGG 0.477445 6.1:-78369974 MS.gene063327:CDS
TTCCATGGCCAGGTATCAAT+TGG 0.480682 6.1:+78370022 None:intergenic
TTTGGCCAAGGAAAGATGCT+TGG 0.483605 6.1:-78369975 MS.gene063327:CDS
GGCGGTAACTTGTCCTCCTA+AGG 0.511225 6.1:-78369847 None:intergenic
AGAACCTGAGATTGAGGTGG+AGG 0.523452 6.1:+78371475 None:intergenic
TGGAGGTTGAAGAGAAGGAG+AGG 0.528382 6.1:+78371492 None:intergenic
AGAGGAATGGTTCCATGGCC+AGG 0.528465 6.1:+78370012 None:intergenic
AGAGATAGAACCTGAGATTG+AGG 0.534734 6.1:+78371469 None:intergenic
GTAATAAGGGCTTAGAGGAA+TGG 0.544994 6.1:+78369999 None:intergenic
AGATGCTTGGGAAGAGCTCA+AGG 0.550724 6.1:-78369962 MS.gene063327:CDS
GTTGTTGTGATGTAGAAGCT+AGG 0.555418 6.1:-78370126 MS.gene063327:intron
TCCTACATTTGAGCCTTAGG+AGG 0.560591 6.1:+78369834 None:intergenic
GGCTTAGAGGAATGGTTCCA+TGG 0.575688 6.1:+78370007 None:intergenic
GGACTTGACCAATTGATACC+TGG 0.579475 6.1:-78370030 MS.gene063327:CDS
GACCAATTGATACCTGGCCA+TGG 0.585251 6.1:-78370024 MS.gene063327:CDS
CCACAAATTGATAATATCAG+TGG 0.611414 6.1:+78369879 None:intergenic
GATAGAACCTGAGATTGAGG+TGG 0.632670 6.1:+78371472 None:intergenic
AGTTGCTAGAGAGTAAGCCG+TGG 0.644993 6.1:-78369939 MS.gene063327:CDS
ATCAATTTGTGGCAGCAGAG+CGG 0.648603 6.1:-78369868 MS.gene063327:CDS
ATTTGGATGGAGAAGAACAT+CGG 0.651745 6.1:-78370057 MS.gene063327:CDS
AATTTGTGGCAGCAGAGCGG+CGG 0.660943 6.1:-78369865 MS.gene063327:CDS
CAAGAGCAGAAACAGTAAAG+CGG 0.684597 6.1:+78371545 None:intergenic
TGTGATGTAGAAGCTAGGAG+TGG 0.712045 6.1:-78370121 MS.gene063327:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAATATAAAACAGTTTTA+GGG - chr6.1:78370460-78370479 MS.gene063327:intron 10.0%
!!! TATTAATATAAAACAGTTTT+AGG - chr6.1:78370459-78370478 MS.gene063327:intron 10.0%
!! AAAATGATTTATGTAAAAGT+GGG - chr6.1:78370210-78370229 MS.gene063327:intron 15.0%
!! TAAAAAGCTTATATTATGTT+TGG + chr6.1:78371236-78371255 None:intergenic 15.0%
!! TAAAATGATTTATGTAAAAG+TGG - chr6.1:78370209-78370228 MS.gene063327:intron 15.0%
!!! ATGAAATTTTAGTTTATGTT+TGG - chr6.1:78370125-78370144 MS.gene063327:intron 15.0%
!!! ATTTATAAGTTTGTTTCAAA+GGG - chr6.1:78369985-78370004 MS.gene063327:CDS 15.0%
!!! CATTTTTGTTTTATAATTGT+TGG - chr6.1:78370553-78370572 MS.gene063327:intron 15.0%
!!! TATTTATAAGTTTGTTTCAA+AGG - chr6.1:78369984-78370003 MS.gene063327:CDS 15.0%
!! ACAAAAATGCTTAAATTACA+GGG + chr6.1:78370440-78370459 None:intergenic 20.0%
!! TACAAAAATGCTTAAATTAC+AGG + chr6.1:78370441-78370460 None:intergenic 20.0%
!! TTCTTAATGAAACTACAAAA+GGG + chr6.1:78370487-78370506 None:intergenic 20.0%
!! TTGAAAAAAATAATCGTGTT+TGG - chr6.1:78370849-78370868 MS.gene063327:intron 20.0%
!! TTTCTTAATGAAACTACAAA+AGG + chr6.1:78370488-78370507 None:intergenic 20.0%
!!! AACTTTTTTAATTGTTACGA+TGG - chr6.1:78371272-78371291 MS.gene063327:intron 20.0%
!!! GTAATAGAAATGATTTTGAA+TGG - chr6.1:78370879-78370898 MS.gene063327:intron 20.0%
!!! TATGATTAAAATTGAGTTGA+AGG - chr6.1:78370186-78370205 MS.gene063327:intron 20.0%
!!! TTTATAAGTTTGTTTCAAAG+GGG - chr6.1:78369986-78370005 MS.gene063327:CDS 20.0%
! ACTAATACACAAGTTTGAAA+GGG - chr6.1:78370777-78370796 MS.gene063327:intron 25.0%
! AGTAGAATCAATTCTATTAG+AGG - chr6.1:78370296-78370315 MS.gene063327:intron 25.0%
! TCTTAATGAAACTACAAAAG+GGG + chr6.1:78370486-78370505 None:intergenic 25.0%
! TTCAATGAAAATGCTTATAG+AGG - chr6.1:78370033-78370052 MS.gene063327:CDS 25.0%
! TTCAGTGTGAATGTTTAAAA+GGG - chr6.1:78370100-78370119 MS.gene063327:CDS 25.0%
! TTGATTTAAACATGTTTGAG+TGG + chr6.1:78371080-78371099 None:intergenic 25.0%
! TTTCAGTGTGAATGTTTAAA+AGG - chr6.1:78370099-78370118 MS.gene063327:CDS 25.0%
! TTTGAGTATAAAATCACCTT+TGG - chr6.1:78370986-78371005 MS.gene063327:intron 25.0%
!! TAACATAGCTGTTGTTATTA+TGG - chr6.1:78370743-78370762 MS.gene063327:intron 25.0%
!! TATCCTTAAGTTTTACCTAA+AGG + chr6.1:78370805-78370824 None:intergenic 25.0%
!!! GTGAATTATTGGTTTATGTT+TGG - chr6.1:78370930-78370949 MS.gene063327:intron 25.0%
!!! TTAGTTTATGTTTGGTTCTA+CGG - chr6.1:78370133-78370152 MS.gene063327:intron 25.0%
AAGTGTAGAAGATTAGAAGT+AGG - chr6.1:78370660-78370679 MS.gene063327:intron 30.0%
CCACTGATATTATCAATTTG+TGG - chr6.1:78371541-78371560 MS.gene063327:CDS 30.0%
CTCTAGATTTGATTCTAACT+TGG + chr6.1:78371035-78371054 None:intergenic 30.0%
GACTAATACACAAGTTTGAA+AGG - chr6.1:78370776-78370795 MS.gene063327:intron 30.0%
GTACCTTTAGGTAAAACTTA+AGG - chr6.1:78370799-78370818 MS.gene063327:intron 30.0%
TGTTTGACCTTCAAAACATT+TGG - chr6.1:78370587-78370606 MS.gene063327:intron 30.0%
! CCACAAATTGATAATATCAG+TGG + chr6.1:78371544-78371563 None:intergenic 30.0%
! GTCACCTATAGAATCAATTT+TGG - chr6.1:78370329-78370348 MS.gene063327:intron 30.0%
! GTGAGTTGAATGTGAATTAT+TGG - chr6.1:78370919-78370938 MS.gene063327:intron 30.0%
!! AAAACATTTGGTGTCGAATA+AGG - chr6.1:78370599-78370618 MS.gene063327:intron 30.0%
!! ATTTGCTTTTGTGATATCCA+CGG + chr6.1:78371501-78371520 None:intergenic 30.0%
!!! TTCATGTGTGTTTGGTTTTA+CGG + chr6.1:78371138-78371157 None:intergenic 30.0%
AACACATATTGTGTCGCATA+CGG - chr6.1:78370698-78370717 MS.gene063327:intron 35.0%
ACACACATGAAACCTATGTT+TGG - chr6.1:78371147-78371166 MS.gene063327:intron 35.0%
ACATAGGTTTCATGTGTGTT+TGG + chr6.1:78371146-78371165 None:intergenic 35.0%
ATTTGATTCTAACTTGGAGC+TGG + chr6.1:78371029-78371048 None:intergenic 35.0%
ATTTGGATGGAGAAGAACAT+CGG - chr6.1:78371363-78371382 MS.gene063327:intron 35.0%
CAAAAGTAATAAGGGCTTAG+AGG + chr6.1:78371429-78371448 None:intergenic 35.0%
CATATGTGTAAGTGTAAGTC+TGG + chr6.1:78370372-78370391 None:intergenic 35.0%
GATTCTATAGGTGACATGTT+TGG + chr6.1:78370324-78370343 None:intergenic 35.0%
TCCTTGGCCAAAAGTAATAA+GGG + chr6.1:78371437-78371456 None:intergenic 35.0%
TTCCTTGGCCAAAAGTAATA+AGG + chr6.1:78371438-78371457 None:intergenic 35.0%
TTTGTAGCATTTGACTCCAA+AGG + chr6.1:78371005-78371024 None:intergenic 35.0%
! CATATCGACTGACATAGAAA+TGG + chr6.1:78371198-78371217 None:intergenic 35.0%
! CTCTAAGCCCTTATTACTTT+TGG - chr6.1:78371427-78371446 MS.gene063327:intron 35.0%
! GGAGCCAAAATTGATTCTAT+AGG + chr6.1:78370336-78370355 None:intergenic 35.0%
!! AAGTTTGAAAGGGTACCTTT+AGG - chr6.1:78370787-78370806 MS.gene063327:intron 35.0%
!! AATAATCGTGTTTGGTTCTG+CGG - chr6.1:78370857-78370876 MS.gene063327:intron 35.0%
!! TGTAAGTCTGGTTTCACTTT+TGG + chr6.1:78370360-78370379 None:intergenic 35.0%
!! TTCGACACCAAATGTTTTGA+AGG + chr6.1:78370597-78370616 None:intergenic 35.0%
AGAGATAGAACCTGAGATTG+AGG + chr6.1:78369954-78369973 None:intergenic 40.0%
ATGAACTTCAACACGAGTCT+TGG + chr6.1:78371345-78371364 None:intergenic 40.0%
ATGGAGAAGAACATCGGTAT+TGG - chr6.1:78371369-78371388 MS.gene063327:intron 40.0%
CAAGAGCAGAAACAGTAAAG+CGG + chr6.1:78369878-78369897 None:intergenic 40.0%
CGTGTTGAAGTTCATTTGGA+TGG - chr6.1:78371350-78371369 MS.gene063327:intron 40.0%
GACTCGTGTTGAAGTTCATT+TGG - chr6.1:78371346-78371365 MS.gene063327:intron 40.0%
GTAATAAGGGCTTAGAGGAA+TGG + chr6.1:78371424-78371443 None:intergenic 40.0%
GTTGTTGTGATGTAGAAGCT+AGG - chr6.1:78371294-78371313 MS.gene063327:intron 40.0%
TAGCTTCTGCATCCAAACAT+AGG + chr6.1:78371162-78371181 None:intergenic 40.0%
!! AAGTCTGGTTTCACTTTTGG+AGG + chr6.1:78370357-78370376 None:intergenic 40.0%
!!! GTGTGTTTGGTTTTACGGTA+GGG + chr6.1:78371133-78371152 None:intergenic 40.0%
!!! TGTGTGTTTGGTTTTACGGT+AGG + chr6.1:78371134-78371153 None:intergenic 40.0%
ATCAATTTGTGGCAGCAGAG+CGG - chr6.1:78371552-78371571 MS.gene063327:CDS 45.0%
GATAGAACCTGAGATTGAGG+TGG + chr6.1:78369951-78369970 None:intergenic 45.0%
GGACTTGACCAATTGATACC+TGG - chr6.1:78371390-78371409 MS.gene063327:intron 45.0%
TCTTCCCAAGCATCTTTCCT+TGG + chr6.1:78371453-78371472 None:intergenic 45.0%
TGTGATGTAGAAGCTAGGAG+TGG - chr6.1:78371299-78371318 MS.gene063327:intron 45.0%
TTCCATGGCCAGGTATCAAT+TGG + chr6.1:78371401-78371420 None:intergenic 45.0%
TTGGCCAAGGAAAGATGCTT+GGG - chr6.1:78371446-78371465 MS.gene063327:intron 45.0%
TTTGGCCAAGGAAAGATGCT+TGG - chr6.1:78371445-78371464 MS.gene063327:intron 45.0%
!! ACGAGTCTTGGTTTGTCTGT+TGG + chr6.1:78371333-78371352 None:intergenic 45.0%
!! GCCCTTATTACTTTTGGCCA+AGG - chr6.1:78371433-78371452 MS.gene063327:intron 45.0%
AGAACCTGAGATTGAGGTGG+AGG + chr6.1:78369948-78369967 None:intergenic 50.0%
AGATGCTTGGGAAGAGCTCA+AGG - chr6.1:78371458-78371477 MS.gene063327:intron 50.0%
AGTTGCTAGAGAGTAAGCCG+TGG - chr6.1:78371481-78371500 MS.gene063327:CDS 50.0%
GACCAATTGATACCTGGCCA+TGG - chr6.1:78371396-78371415 MS.gene063327:intron 50.0%
GGCTTAGAGGAATGGTTCCA+TGG + chr6.1:78371416-78371435 None:intergenic 50.0%
TCAACCTCCACCTCAATCTC+AGG - chr6.1:78369941-78369960 MS.gene063327:CDS 50.0%
TGAGGTGGAGGTTGAAGAGA+AGG + chr6.1:78369936-78369955 None:intergenic 50.0%
TGGAGGTTGAAGAGAAGGAG+AGG + chr6.1:78369931-78369950 None:intergenic 50.0%
AATTTGTGGCAGCAGAGCGG+CGG - chr6.1:78371555-78371574 MS.gene063327:CDS 55.0%
AGAGGAATGGTTCCATGGCC+AGG + chr6.1:78371411-78371430 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 78369849 78371593 78369849 ID=MS.gene063327
chr6.1 mRNA 78369849 78371593 78369849 ID=MS.gene063327.t1;Parent=MS.gene063327
chr6.1 exon 78371480 78371593 78371480 ID=MS.gene063327.t1.exon1;Parent=MS.gene063327.t1
chr6.1 CDS 78371480 78371593 78371480 ID=cds.MS.gene063327.t1;Parent=MS.gene063327.t1
chr6.1 exon 78369849 78370133 78369849 ID=MS.gene063327.t1.exon2;Parent=MS.gene063327.t1
chr6.1 CDS 78369849 78370133 78369849 ID=cds.MS.gene063327.t1;Parent=MS.gene063327.t1
Gene Sequence

>MS.gene063327

ATGAACAAGTTGAAGATGAAAACTAACCGCTTTACTGTTTCTGCTCTTGTTGCTGAAGATGCTGCTGTTTCTTCTCAATCCTCTCCTTCTCTTCAACCTCCACCTCAATCTCAGGTTCTATCTCTTTCTAGCACTTATTTATAAGTTTGTTTCAAAGGGGTGCTTAACTTGTTATTGTTGTAATTTCAATGAAAATGCTTATAGAGGTGATGAATTTTTTGTGTTGATAAACAGAAACATGTTATTGTAATTTCAGTGTGAATGTTTAAAAGGGTGATGAAATTTTAGTTTATGTTTGGTTCTACGGTGAGAAAATTTGATTTTTGAGTGAATTGATTATGATTAAAATTGAGTTGAAGGTAAAATGATTTATGTAAAAGTGGGTTTAACAATAAGTTTGATTGTAAAAATTAATTCTAGAATGAGAAGCTACATTTTCTAGCTTCAAGTAGAATCAATTCTATTAGAGGAACCAAACATGTCACCTATAGAATCAATTTTGGCTCCTCCAAAAGTGAAACCAGACTTACACTTACACATATGATATTGTTATCTCTGATTATTGAAATCTTAAAAAATGATTTTTCTCCCTGTAATTTAAGCATTTTTGTATTAATATAAAACAGTTTTAGGGCCCCTTTTGTAGTTTCATTAAGAAATGAAATCTTTAAAAAGTGTTTTTTTTTTTCTTTCTCTAATTTAAGCATTTTTGTTTTATAATTGTTGGTAGAGAAATGATGTTTGACCTTCAAAACATTTGGTGTCGAATAAGGTGTGAGTGTTTTTTGTGTGTATTTTTTAAATGTGTGTAAAGTGTAGAAGATTAGAAGTAGGAGAGAGATACAGATAAACACATATTGTGTCGCATACGGTGTTAGTGTTTTGATGTGCAAGTAACATAGCTGTTGTTATTATGGTTGTGATGTAGACTAATACACAAGTTTGAAAGGGTACCTTTAGGTAAAACTTAAGGATATTACTATGTCTGATATTTGAAATATTGAAAAAAATAATCGTGTTTGGTTCTGCGGTAATAGAAATGATTTTGAATGGATTGATTTGAGTTTAAAGTGAGTTGAATGTGAATTATTGGTTTATGTTTGGATACAATCATGTAAACATGAATTGAACAATAAATTTGAGTATAAAATCACCTTTGGAGTCAAATGCTACAAATCCAGCTCCAAGTTAGAATCAAATCTAGAGAACAAATCAATTTCAATAGACACCACTCAAACATGTTTAAATCAATTTTATGTGTCTAGAATCACTTTTGACTCTCCCTACCGTAAAACCAAACACACATGAAACCTATGTTTGGATGCAGAAGCTACAACTACAAGCTTCCATTTCTATGTCAGTCGATATGCATGTGATCGTGAATCCAAACATAATATAAGCTTTTTAGTTTCTCTTATTTAAGAACTTTTTTAATTGTTACGATGGTTGTTGTGATGTAGAAGCTAGGAGTGGTTGTGAAACCAACAGACAAACCAAGACTCGTGTTGAAGTTCATTTGGATGGAGAAGAACATCGGTATTGGACTTGACCAATTGATACCTGGCCATGGAACCATTCCTCTAAGCCCTTATTACTTTTGGCCAAGGAAAGATGCTTGGGAAGAGCTCAAGGAGTTGCTAGAGAGTAAGCCGTGGATATCACAAAAGCAAATGATTATTCTTCTCAATCAAGCCACTGATATTATCAATTTGTGGCAGCAGAGCGGCGGTAACTTGTCCTCCTAA

Protein sequence

>MS.gene063327.t1

MNKLKMKTNRFTVSALVAEDAAVSSQSSPSLQPPPQSQKLGVVVKPTDKPRLVLKFIWMEKNIGIGLDQLIPGHGTIPLSPYYFWPRKDAWEELKELLESKPWISQKQMIILLNQATDIINLWQQSGGNLSS