Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065145.t1 | XP_003592651.1 | 94.7 | 131 | 7 | 0 | 1 | 131 | 60 | 190 | 1.20E-61 | 245.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065145.t1 | G7ID96 | 94.7 | 131 | 7 | 0 | 1 | 131 | 60 | 190 | 8.3e-62 | 245.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene065145 | -0.833048 | 6.67E-56 | -1.69E-46 |
| MS.gene049217 | MS.gene065145 | -0.804224 | 2.39E-49 | -1.69E-46 |
| MS.gene049222 | MS.gene065145 | -0.812774 | 3.58E-51 | -1.69E-46 |
| MS.gene049281 | MS.gene065145 | 0.828915 | 6.89E-55 | -1.69E-46 |
| MS.gene049285 | MS.gene065145 | -0.820325 | 7.28E-53 | -1.69E-46 |
| MS.gene049312 | MS.gene065145 | 0.821046 | 4.97E-53 | -1.69E-46 |
| MS.gene049320 | MS.gene065145 | -0.825717 | 4.03E-54 | -1.69E-46 |
| MS.gene049611 | MS.gene065145 | 0.808494 | 3.01E-50 | -1.69E-46 |
| MS.gene050095 | MS.gene065145 | 0.810049 | 1.40E-50 | -1.69E-46 |
| MS.gene050170 | MS.gene065145 | -0.802597 | 5.20E-49 | -1.69E-46 |
| MS.gene050363 | MS.gene065145 | 0.808503 | 3.00E-50 | -1.69E-46 |
| MS.gene050381 | MS.gene065145 | 0.869577 | 2.79E-66 | -1.69E-46 |
| MS.gene050504 | MS.gene065145 | -0.841083 | 5.89E-58 | -1.69E-46 |
| MS.gene050612 | MS.gene065145 | -0.82768 | 1.37E-54 | -1.69E-46 |
| MS.gene050675 | MS.gene065145 | -0.814152 | 1.78E-51 | -1.69E-46 |
| MS.gene050699 | MS.gene065145 | -0.815378 | 9.53E-52 | -1.69E-46 |
| MS.gene050772 | MS.gene065145 | -0.802763 | 4.81E-49 | -1.69E-46 |
| MS.gene05080 | MS.gene065145 | -0.818652 | 1.75E-52 | -1.69E-46 |
| MS.gene050868 | MS.gene065145 | -0.840184 | 1.01E-57 | -1.69E-46 |
| MS.gene050871 | MS.gene065145 | -0.838554 | 2.68E-57 | -1.69E-46 |
| MS.gene050883 | MS.gene065145 | 0.827222 | 1.76E-54 | -1.69E-46 |
| MS.gene050986 | MS.gene065145 | -0.813515 | 2.46E-51 | -1.69E-46 |
| MS.gene051044 | MS.gene065145 | 0.845433 | 4.07E-59 | -1.69E-46 |
| MS.gene05104 | MS.gene065145 | 0.828962 | 6.71E-55 | -1.69E-46 |
| MS.gene051127 | MS.gene065145 | 0.824675 | 7.10E-54 | -1.69E-46 |
| MS.gene051138 | MS.gene065145 | 0.833553 | 4.99E-56 | -1.69E-46 |
| MS.gene051364 | MS.gene065145 | 0.819513 | 1.12E-52 | -1.69E-46 |
| MS.gene05141 | MS.gene065145 | 0.811145 | 8.11E-51 | -1.69E-46 |
| MS.gene051694 | MS.gene065145 | -0.812166 | 4.86E-51 | -1.69E-46 |
| MS.gene051946 | MS.gene065145 | 0.848896 | 4.57E-60 | -1.69E-46 |
| MS.gene051973 | MS.gene065145 | 0.802592 | 5.21E-49 | -1.69E-46 |
| MS.gene052164 | MS.gene065145 | -0.829788 | 4.23E-55 | -1.69E-46 |
| MS.gene052211 | MS.gene065145 | 0.82779 | 1.29E-54 | -1.69E-46 |
| MS.gene05240 | MS.gene065145 | 0.802103 | 6.58E-49 | -1.69E-46 |
| MS.gene052478 | MS.gene065145 | 0.805266 | 1.45E-49 | -1.69E-46 |
| MS.gene052486 | MS.gene065145 | -0.807326 | 5.34E-50 | -1.69E-46 |
| MS.gene052503 | MS.gene065145 | -0.810715 | 1.00E-50 | -1.69E-46 |
| MS.gene052802 | MS.gene065145 | 0.807662 | 4.53E-50 | -1.69E-46 |
| MS.gene052823 | MS.gene065145 | -0.809948 | 1.47E-50 | -1.69E-46 |
| MS.gene052825 | MS.gene065145 | -0.810316 | 1.22E-50 | -1.69E-46 |
| MS.gene052966 | MS.gene065145 | -0.818938 | 1.51E-52 | -1.69E-46 |
| MS.gene053203 | MS.gene065145 | -0.828642 | 8.03E-55 | -1.69E-46 |
| MS.gene053213 | MS.gene065145 | -0.808936 | 2.42E-50 | -1.69E-46 |
| MS.gene053286 | MS.gene065145 | -0.804611 | 1.99E-49 | -1.69E-46 |
| MS.gene053420 | MS.gene065145 | 0.832253 | 1.05E-55 | -1.69E-46 |
| MS.gene053488 | MS.gene065145 | 0.834288 | 3.27E-56 | -1.69E-46 |
| MS.gene053549 | MS.gene065145 | 0.84967 | 2.79E-60 | -1.69E-46 |
| MS.gene053561 | MS.gene065145 | 0.836969 | 6.85E-57 | -1.69E-46 |
| MS.gene053574 | MS.gene065145 | -0.808847 | 2.53E-50 | -1.69E-46 |
| MS.gene053824 | MS.gene065145 | -0.8038 | 2.93E-49 | -1.69E-46 |
| MS.gene053887 | MS.gene065145 | 0.814 | 1.93E-51 | -1.69E-46 |
| MS.gene054054 | MS.gene065145 | 0.83283 | 7.56E-56 | -1.69E-46 |
| MS.gene054146 | MS.gene065145 | 0.808264 | 3.37E-50 | -1.69E-46 |
| MS.gene054642 | MS.gene065145 | -0.809999 | 1.43E-50 | -1.69E-46 |
| MS.gene054706 | MS.gene065145 | 0.812099 | 5.03E-51 | -1.69E-46 |
| MS.gene054708 | MS.gene065145 | 0.822887 | 1.86E-53 | -1.69E-46 |
| MS.gene05470 | MS.gene065145 | -0.821794 | 3.34E-53 | -1.69E-46 |
| MS.gene054954 | MS.gene065145 | -0.816381 | 5.70E-52 | -1.69E-46 |
| MS.gene054959 | MS.gene065145 | 0.847227 | 1.32E-59 | -1.69E-46 |
| MS.gene055124 | MS.gene065145 | 0.833602 | 4.85E-56 | -1.69E-46 |
| MS.gene055154 | MS.gene065145 | 0.846655 | 1.89E-59 | -1.69E-46 |
| MS.gene055202 | MS.gene065145 | 0.815799 | 7.68E-52 | -1.69E-46 |
| MS.gene055362 | MS.gene065145 | -0.83307 | 6.59E-56 | -1.69E-46 |
| MS.gene055502 | MS.gene065145 | -0.856431 | 3.23E-62 | -1.69E-46 |
| MS.gene055577 | MS.gene065145 | -0.846351 | 2.29E-59 | -1.69E-46 |
| MS.gene05589 | MS.gene065145 | -0.828239 | 1.00E-54 | -1.69E-46 |
| MS.gene055942 | MS.gene065145 | 0.800788 | 1.22E-48 | -1.69E-46 |
| MS.gene056224 | MS.gene065145 | 0.804 | 2.66E-49 | -1.69E-46 |
| MS.gene056310 | MS.gene065145 | 0.825516 | 4.49E-54 | -1.69E-46 |
| MS.gene056311 | MS.gene065145 | 0.837414 | 5.27E-57 | -1.69E-46 |
| MS.gene056313 | MS.gene065145 | 0.82327 | 1.52E-53 | -1.69E-46 |
| MS.gene056408 | MS.gene065145 | 0.818334 | 2.07E-52 | -1.69E-46 |
| MS.gene056504 | MS.gene065145 | -0.821695 | 3.52E-53 | -1.69E-46 |
| MS.gene056510 | MS.gene065145 | -0.805494 | 1.30E-49 | -1.69E-46 |
| MS.gene056550 | MS.gene065145 | 0.82222 | 2.66E-53 | -1.69E-46 |
| MS.gene056657 | MS.gene065145 | -0.807214 | 5.64E-50 | -1.69E-46 |
| MS.gene056748 | MS.gene065145 | 0.800039 | 1.74E-48 | -1.69E-46 |
| MS.gene056856 | MS.gene065145 | 0.801442 | 8.99E-49 | -1.69E-46 |
| MS.gene056988 | MS.gene065145 | 0.808393 | 3.17E-50 | -1.69E-46 |
| MS.gene057014 | MS.gene065145 | 0.867249 | 1.57E-65 | -1.69E-46 |
| MS.gene058068 | MS.gene065145 | 0.826523 | 2.59E-54 | -1.69E-46 |
| MS.gene058099 | MS.gene065145 | 0.801668 | 8.08E-49 | -1.69E-46 |
| MS.gene058117 | MS.gene065145 | 0.807499 | 4.91E-50 | -1.69E-46 |
| MS.gene058268 | MS.gene065145 | 0.803486 | 3.41E-49 | -1.69E-46 |
| MS.gene058269 | MS.gene065145 | 0.801928 | 7.14E-49 | -1.69E-46 |
| MS.gene058334 | MS.gene065145 | 0.806289 | 8.84E-50 | -1.69E-46 |
| MS.gene05837 | MS.gene065145 | -0.839039 | 2.01E-57 | -1.69E-46 |
| MS.gene058419 | MS.gene065145 | -0.826052 | 3.35E-54 | -1.69E-46 |
| MS.gene058544 | MS.gene065145 | -0.83782 | 4.15E-57 | -1.69E-46 |
| MS.gene058603 | MS.gene065145 | 0.840835 | 6.84E-58 | -1.69E-46 |
| MS.gene058773 | MS.gene065145 | -0.827656 | 1.39E-54 | -1.69E-46 |
| MS.gene058838 | MS.gene065145 | -0.801416 | 9.10E-49 | -1.69E-46 |
| MS.gene059022 | MS.gene065145 | -0.84287 | 1.98E-58 | -1.69E-46 |
| MS.gene059023 | MS.gene065145 | -0.839145 | 1.89E-57 | -1.69E-46 |
| MS.gene059223 | MS.gene065145 | 0.838464 | 2.83E-57 | -1.69E-46 |
| MS.gene059677 | MS.gene065145 | -0.823341 | 1.46E-53 | -1.69E-46 |
| MS.gene059690 | MS.gene065145 | 0.807128 | 5.88E-50 | -1.69E-46 |
| MS.gene059691 | MS.gene065145 | 0.818927 | 1.52E-52 | -1.69E-46 |
| MS.gene059694 | MS.gene065145 | 0.826644 | 2.42E-54 | -1.69E-46 |
| MS.gene059697 | MS.gene065145 | 0.838645 | 2.54E-57 | -1.69E-46 |
| MS.gene059718 | MS.gene065145 | -0.841097 | 5.84E-58 | -1.69E-46 |
| MS.gene059865 | MS.gene065145 | -0.823898 | 1.08E-53 | -1.69E-46 |
| MS.gene059902 | MS.gene065145 | -0.80755 | 4.79E-50 | -1.69E-46 |
| MS.gene059916 | MS.gene065145 | 0.852682 | 3.93E-61 | -1.69E-46 |
| MS.gene060595 | MS.gene065145 | 0.807713 | 4.42E-50 | -1.69E-46 |
| MS.gene060762 | MS.gene065145 | -0.826069 | 3.32E-54 | -1.69E-46 |
| MS.gene06077 | MS.gene065145 | -0.842983 | 1.85E-58 | -1.69E-46 |
| MS.gene06092 | MS.gene065145 | -0.807886 | 4.06E-50 | -1.69E-46 |
| MS.gene060982 | MS.gene065145 | -0.813608 | 2.35E-51 | -1.69E-46 |
| MS.gene061044 | MS.gene065145 | 0.80742 | 5.10E-50 | -1.69E-46 |
| MS.gene061150 | MS.gene065145 | -0.805131 | 1.55E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065145.t1 | MTR_1g110570 | 94.656 | 131 | 7 | 0 | 1 | 131 | 60 | 190 | 5.11e-88 | 254 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATTCTCCATTTGGGATTT+TGG | 0.076776 | 1.2:-81888287 | MS.gene065145:CDS |
| GGAAGTTTGATATCTCCTTC+TGG | 0.304807 | 1.2:-81888265 | MS.gene065145:CDS |
| TTGATATCTCCTTCTGGATT+GGG | 0.350395 | 1.2:-81888259 | MS.gene065145:CDS |
| CAACTAGCTCGGTTATTAAG+AGG | 0.383122 | 1.2:-81888474 | MS.gene065145:CDS |
| GCAAACTTCATAAGATTAGA+AGG | 0.390517 | 1.2:+81888385 | None:intergenic |
| TCTGATGGATCAAACAATAA+TGG | 0.435632 | 1.2:-81888511 | MS.gene065145:CDS |
| CAGAAGAAATCATGTGAAGT+TGG | 0.437694 | 1.2:+81888343 | None:intergenic |
| TTTGATATCTCCTTCTGGAT+TGG | 0.442159 | 1.2:-81888260 | MS.gene065145:CDS |
| AAAGTGAAGAATTAATATTC+AGG | 0.448396 | 1.2:+81888220 | None:intergenic |
| TTTGAGTCATCTTTGTCTGA+TGG | 0.458174 | 1.2:-81888526 | MS.gene065145:CDS |
| TTATACACTGGTCGTGATGA+TGG | 0.466944 | 1.2:+81888316 | None:intergenic |
| TGTGAAGTTGGAGTGAATAA+TGG | 0.471016 | 1.2:+81888355 | None:intergenic |
| ATTGATGAGAACTGTGATAA+GGG | 0.477546 | 1.2:-81888442 | MS.gene065145:CDS |
| AAACTTCCCAAAATCCCAAA+TGG | 0.491558 | 1.2:+81888280 | None:intergenic |
| CTGATGTGGCTGTACTTGAT+TGG | 0.491716 | 1.2:+81888567 | None:intergenic |
| TATTGATGAGAACTGTGATA+AGG | 0.522044 | 1.2:-81888443 | MS.gene065145:CDS |
| TGGCTGTACTTGATTGGTAG+TGG | 0.532172 | 1.2:+81888573 | None:intergenic |
| AACTAGCTCGGTTATTAAGA+GGG | 0.536352 | 1.2:-81888473 | MS.gene065145:CDS |
| TGAAACTTCTTCAACTAGCT+CGG | 0.546440 | 1.2:-81888485 | MS.gene065145:CDS |
| GCTCTTGTTCAAAGTCTCAC+AGG | 0.558158 | 1.2:-81888601 | MS.gene065145:CDS |
| TCAGAGACTTCTAGCTGATG+TGG | 0.588032 | 1.2:+81888553 | None:intergenic |
| ATTAATATTCAGGTAGATCG+TGG | 0.588111 | 1.2:+81888230 | None:intergenic |
| GAATCAGAAAATTTATACAC+TGG | 0.610328 | 1.2:+81888304 | None:intergenic |
| TGGATGAATCCCAATCCAGA+AGG | 0.659766 | 1.2:+81888250 | None:intergenic |
| GATGAGAACTGTGATAAGGG+TGG | 0.703456 | 1.2:-81888439 | MS.gene065145:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GAATCAGAAAATTTATACAC+TGG | + | chr1.2:81888534-81888553 | None:intergenic | 25.0% |
| !! | AAATTTTCTGATTCTCCATT+TGG | - | chr1.2:81888540-81888559 | MS.gene065145:CDS | 25.0% |
| !! | AATTTTCTGATTCTCCATTT+GGG | - | chr1.2:81888541-81888560 | MS.gene065145:CDS | 25.0% |
| ATTGATGAGAACTGTGATAA+GGG | - | chr1.2:81888393-81888412 | MS.gene065145:CDS | 30.0% | |
| GCAAACTTCATAAGATTAGA+AGG | + | chr1.2:81888453-81888472 | None:intergenic | 30.0% | |
| TATTGATGAGAACTGTGATA+AGG | - | chr1.2:81888392-81888411 | MS.gene065145:CDS | 30.0% | |
| ! | TCTGATGGATCAAACAATAA+TGG | - | chr1.2:81888324-81888343 | MS.gene065145:CDS | 30.0% |
| AAACTTCCCAAAATCCCAAA+TGG | + | chr1.2:81888558-81888577 | None:intergenic | 35.0% | |
| AACTAGCTCGGTTATTAAGA+GGG | - | chr1.2:81888362-81888381 | MS.gene065145:CDS | 35.0% | |
| CAGAAGAAATCATGTGAAGT+TGG | + | chr1.2:81888495-81888514 | None:intergenic | 35.0% | |
| TGAAACTTCTTCAACTAGCT+CGG | - | chr1.2:81888350-81888369 | MS.gene065145:CDS | 35.0% | |
| TGTGAAGTTGGAGTGAATAA+TGG | + | chr1.2:81888483-81888502 | None:intergenic | 35.0% | |
| TTGATATCTCCTTCTGGATT+GGG | - | chr1.2:81888576-81888595 | MS.gene065145:CDS | 35.0% | |
| TTTGAGTCATCTTTGTCTGA+TGG | - | chr1.2:81888309-81888328 | MS.gene065145:CDS | 35.0% | |
| TTTGATATCTCCTTCTGGAT+TGG | - | chr1.2:81888575-81888594 | MS.gene065145:CDS | 35.0% | |
| !! | GATTCTCCATTTGGGATTTT+GGG | - | chr1.2:81888549-81888568 | MS.gene065145:CDS | 35.0% |
| !! | TGATTCTCCATTTGGGATTT+TGG | - | chr1.2:81888548-81888567 | MS.gene065145:CDS | 35.0% |
| CAACTAGCTCGGTTATTAAG+AGG | - | chr1.2:81888361-81888380 | MS.gene065145:CDS | 40.0% | |
| GGAAGTTTGATATCTCCTTC+TGG | - | chr1.2:81888570-81888589 | MS.gene065145:CDS | 40.0% | |
| TTATACACTGGTCGTGATGA+TGG | + | chr1.2:81888522-81888541 | None:intergenic | 40.0% | |
| CTGATGTGGCTGTACTTGAT+TGG | + | chr1.2:81888271-81888290 | None:intergenic | 45.0% | |
| GATGAGAACTGTGATAAGGG+TGG | - | chr1.2:81888396-81888415 | MS.gene065145:CDS | 45.0% | |
| GCTCTTGTTCAAAGTCTCAC+AGG | - | chr1.2:81888234-81888253 | MS.gene065145:CDS | 45.0% | |
| TGGATGAATCCCAATCCAGA+AGG | + | chr1.2:81888588-81888607 | None:intergenic | 45.0% | |
| TGGCTGTACTTGATTGGTAG+TGG | + | chr1.2:81888265-81888284 | None:intergenic | 45.0% | |
| ! | TCAGAGACTTCTAGCTGATG+TGG | + | chr1.2:81888285-81888304 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 81888231 | 81888626 | 81888231 | ID=MS.gene065145 |
| chr1.2 | mRNA | 81888231 | 81888626 | 81888231 | ID=MS.gene065145.t1;Parent=MS.gene065145 |
| chr1.2 | exon | 81888231 | 81888626 | 81888231 | ID=MS.gene065145.t1.exon1;Parent=MS.gene065145.t1 |
| chr1.2 | CDS | 81888231 | 81888626 | 81888231 | ID=cds.MS.gene065145.t1;Parent=MS.gene065145.t1 |
| Gene Sequence |
| Protein sequence |
