Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06921.t1 | XP_013461420.1 | 97.5 | 276 | 7 | 0 | 1 | 276 | 56 | 331 | 8.20E-150 | 539.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06921.t1 | Q94A79 | 61.8 | 275 | 103 | 1 | 2 | 276 | 66 | 338 | 5.4e-94 | 345.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06921.t1 | A0A072VBA8 | 97.5 | 276 | 7 | 0 | 1 | 276 | 56 | 331 | 5.9e-150 | 539.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene06921 | 0.847889 | 8.69E-60 | -1.69E-46 |
| MS.gene049378 | MS.gene06921 | 0.809945 | 1.47E-50 | -1.69E-46 |
| MS.gene049382 | MS.gene06921 | 0.81707 | 3.99E-52 | -1.69E-46 |
| MS.gene049435 | MS.gene06921 | 0.829268 | 5.66E-55 | -1.69E-46 |
| MS.gene049501 | MS.gene06921 | 0.817092 | 3.94E-52 | -1.69E-46 |
| MS.gene049850 | MS.gene06921 | 0.811674 | 6.22E-51 | -1.69E-46 |
| MS.gene050020 | MS.gene06921 | 0.81217 | 4.85E-51 | -1.69E-46 |
| MS.gene050226 | MS.gene06921 | 0.852767 | 3.72E-61 | -1.69E-46 |
| MS.gene050227 | MS.gene06921 | 0.849148 | 3.90E-60 | -1.69E-46 |
| MS.gene050228 | MS.gene06921 | 0.847816 | 9.10E-60 | -1.69E-46 |
| MS.gene050237 | MS.gene06921 | 0.8037 | 3.08E-49 | -1.69E-46 |
| MS.gene050303 | MS.gene06921 | 0.801358 | 9.35E-49 | -1.69E-46 |
| MS.gene050438 | MS.gene06921 | 0.861177 | 1.23E-63 | -1.69E-46 |
| MS.gene050442 | MS.gene06921 | 0.865172 | 7.16E-65 | -1.69E-46 |
| MS.gene050516 | MS.gene06921 | 0.80362 | 3.20E-49 | -1.69E-46 |
| MS.gene050558 | MS.gene06921 | 0.806947 | 6.42E-50 | -1.69E-46 |
| MS.gene050586 | MS.gene06921 | 0.808756 | 2.65E-50 | -1.69E-46 |
| MS.gene050623 | MS.gene06921 | -0.830531 | 2.79E-55 | -1.69E-46 |
| MS.gene050680 | MS.gene06921 | 0.804062 | 2.59E-49 | -1.69E-46 |
| MS.gene050719 | MS.gene06921 | 0.866104 | 3.64E-65 | -1.69E-46 |
| MS.gene050721 | MS.gene06921 | 0.865446 | 5.87E-65 | -1.69E-46 |
| MS.gene050724 | MS.gene06921 | 0.863505 | 2.37E-64 | -1.69E-46 |
| MS.gene050780 | MS.gene06921 | 0.826799 | 2.22E-54 | -1.69E-46 |
| MS.gene050787 | MS.gene06921 | 0.811512 | 6.75E-51 | -1.69E-46 |
| MS.gene050807 | MS.gene06921 | 0.810711 | 1.01E-50 | -1.69E-46 |
| MS.gene050820 | MS.gene06921 | 0.833344 | 5.63E-56 | -1.69E-46 |
| MS.gene050824 | MS.gene06921 | 0.832888 | 7.31E-56 | -1.69E-46 |
| MS.gene050844 | MS.gene06921 | 0.820987 | 5.13E-53 | -1.69E-46 |
| MS.gene050959 | MS.gene06921 | 0.82071 | 5.94E-53 | -1.69E-46 |
| MS.gene051247 | MS.gene06921 | 0.819165 | 1.34E-52 | -1.69E-46 |
| MS.gene051265 | MS.gene06921 | 0.836278 | 1.03E-56 | -1.69E-46 |
| MS.gene051344 | MS.gene06921 | 0.809976 | 1.45E-50 | -1.69E-46 |
| MS.gene051860 | MS.gene06921 | 0.817951 | 2.53E-52 | -1.69E-46 |
| MS.gene052012 | MS.gene06921 | 0.809481 | 1.85E-50 | -1.69E-46 |
| MS.gene052020 | MS.gene06921 | 0.866891 | 2.05E-65 | -1.69E-46 |
| MS.gene052176 | MS.gene06921 | 0.801676 | 8.05E-49 | -1.69E-46 |
| MS.gene052255 | MS.gene06921 | 0.805222 | 1.48E-49 | -1.69E-46 |
| MS.gene052323 | MS.gene06921 | 0.848207 | 7.10E-60 | -1.69E-46 |
| MS.gene052611 | MS.gene06921 | 0.839045 | 2.00E-57 | -1.69E-46 |
| MS.gene052612 | MS.gene06921 | 0.802234 | 6.18E-49 | -1.69E-46 |
| MS.gene052613 | MS.gene06921 | 0.817283 | 3.57E-52 | -1.69E-46 |
| MS.gene052665 | MS.gene06921 | 0.834868 | 2.34E-56 | -1.69E-46 |
| MS.gene052773 | MS.gene06921 | 0.816102 | 6.58E-52 | -1.69E-46 |
| MS.gene052856 | MS.gene06921 | 0.816491 | 5.38E-52 | -1.69E-46 |
| MS.gene052858 | MS.gene06921 | 0.80045 | 1.43E-48 | -1.69E-46 |
| MS.gene052860 | MS.gene06921 | 0.817378 | 3.40E-52 | -1.69E-46 |
| MS.gene052863 | MS.gene06921 | 0.824863 | 6.41E-54 | -1.69E-46 |
| MS.gene052896 | MS.gene06921 | 0.841738 | 3.96E-58 | -1.69E-46 |
| MS.gene053008 | MS.gene06921 | 0.834787 | 2.45E-56 | -1.69E-46 |
| MS.gene053074 | MS.gene06921 | 0.822581 | 2.19E-53 | -1.69E-46 |
| MS.gene053216 | MS.gene06921 | 0.801329 | 9.48E-49 | -1.69E-46 |
| MS.gene053221 | MS.gene06921 | 0.816948 | 4.25E-52 | -1.69E-46 |
| MS.gene053226 | MS.gene06921 | 0.801904 | 7.23E-49 | -1.69E-46 |
| MS.gene053630 | MS.gene06921 | 0.805871 | 1.08E-49 | -1.69E-46 |
| MS.gene053811 | MS.gene06921 | 0.807601 | 4.67E-50 | -1.69E-46 |
| MS.gene053836 | MS.gene06921 | 0.802841 | 4.63E-49 | -1.69E-46 |
| MS.gene053966 | MS.gene06921 | 0.803188 | 3.93E-49 | -1.69E-46 |
| MS.gene05414 | MS.gene06921 | 0.808875 | 2.50E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene06921 | 0.827136 | 1.85E-54 | -1.69E-46 |
| MS.gene054184 | MS.gene06921 | 0.824255 | 8.91E-54 | -1.69E-46 |
| MS.gene054185 | MS.gene06921 | 0.830427 | 2.96E-55 | -1.69E-46 |
| MS.gene054236 | MS.gene06921 | 0.814166 | 1.77E-51 | -1.69E-46 |
| MS.gene054467 | MS.gene06921 | 0.835114 | 2.03E-56 | -1.69E-46 |
| MS.gene054468 | MS.gene06921 | 0.81752 | 3.16E-52 | -1.69E-46 |
| MS.gene054472 | MS.gene06921 | 0.813734 | 2.20E-51 | -1.69E-46 |
| MS.gene054596 | MS.gene06921 | 0.81188 | 5.61E-51 | -1.69E-46 |
| MS.gene05462 | MS.gene06921 | 0.803862 | 2.85E-49 | -1.69E-46 |
| MS.gene05468 | MS.gene06921 | 0.812284 | 4.58E-51 | -1.69E-46 |
| MS.gene054930 | MS.gene06921 | 0.80105 | 1.08E-48 | -1.69E-46 |
| MS.gene054942 | MS.gene06921 | 0.809139 | 2.19E-50 | -1.69E-46 |
| MS.gene05510 | MS.gene06921 | 0.820806 | 5.65E-53 | -1.69E-46 |
| MS.gene055356 | MS.gene06921 | 0.813406 | 2.60E-51 | -1.69E-46 |
| MS.gene055358 | MS.gene06921 | 0.802515 | 5.41E-49 | -1.69E-46 |
| MS.gene055363 | MS.gene06921 | 0.811209 | 7.85E-51 | -1.69E-46 |
| MS.gene055608 | MS.gene06921 | 0.817787 | 2.75E-52 | -1.69E-46 |
| MS.gene055778 | MS.gene06921 | 0.821439 | 4.03E-53 | -1.69E-46 |
| MS.gene055818 | MS.gene06921 | 0.83798 | 3.77E-57 | -1.69E-46 |
| MS.gene055926 | MS.gene06921 | 0.801093 | 1.06E-48 | -1.69E-46 |
| MS.gene05604 | MS.gene06921 | 0.819087 | 1.40E-52 | -1.69E-46 |
| MS.gene056069 | MS.gene06921 | -0.802332 | 5.90E-49 | -1.69E-46 |
| MS.gene056173 | MS.gene06921 | 0.825897 | 3.65E-54 | -1.69E-46 |
| MS.gene056506 | MS.gene06921 | 0.83185 | 1.32E-55 | -1.69E-46 |
| MS.gene056553 | MS.gene06921 | 0.807864 | 4.10E-50 | -1.69E-46 |
| MS.gene056760 | MS.gene06921 | 0.812291 | 4.56E-51 | -1.69E-46 |
| MS.gene057026 | MS.gene06921 | 0.81236 | 4.41E-51 | -1.69E-46 |
| MS.gene057060 | MS.gene06921 | 0.800066 | 1.72E-48 | -1.69E-46 |
| MS.gene057414 | MS.gene06921 | 0.801446 | 8.97E-49 | -1.69E-46 |
| MS.gene057469 | MS.gene06921 | 0.811148 | 8.09E-51 | -1.69E-46 |
| MS.gene057750 | MS.gene06921 | 0.827439 | 1.56E-54 | -1.69E-46 |
| MS.gene057869 | MS.gene06921 | 0.841898 | 3.59E-58 | -1.69E-46 |
| MS.gene057915 | MS.gene06921 | 0.858203 | 9.68E-63 | -1.69E-46 |
| MS.gene058084 | MS.gene06921 | 0.814318 | 1.64E-51 | -1.69E-46 |
| MS.gene058139 | MS.gene06921 | 0.809967 | 1.46E-50 | -1.69E-46 |
| MS.gene058168 | MS.gene06921 | 0.847905 | 8.60E-60 | -1.69E-46 |
| MS.gene058399 | MS.gene06921 | 0.802478 | 5.50E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene06921 | 0.813713 | 2.23E-51 | -1.69E-46 |
| MS.gene058636 | MS.gene06921 | 0.815776 | 7.77E-52 | -1.69E-46 |
| MS.gene058906 | MS.gene06921 | 0.815039 | 1.13E-51 | -1.69E-46 |
| MS.gene058979 | MS.gene06921 | 0.808566 | 2.91E-50 | -1.69E-46 |
| MS.gene059124 | MS.gene06921 | 0.837969 | 3.80E-57 | -1.69E-46 |
| MS.gene059128 | MS.gene06921 | 0.826203 | 3.09E-54 | -1.69E-46 |
| MS.gene059161 | MS.gene06921 | 0.804317 | 2.29E-49 | -1.69E-46 |
| MS.gene059220 | MS.gene06921 | 0.807786 | 4.26E-50 | -1.69E-46 |
| MS.gene059249 | MS.gene06921 | 0.813661 | 2.29E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene06921 | 0.801836 | 7.46E-49 | -1.69E-46 |
| MS.gene059713 | MS.gene06921 | 0.80005 | 1.73E-48 | -1.69E-46 |
| MS.gene059725 | MS.gene06921 | 0.821815 | 3.30E-53 | -1.69E-46 |
| MS.gene05973 | MS.gene06921 | 0.802413 | 5.68E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene06921 | 0.820542 | 6.49E-53 | -1.69E-46 |
| MS.gene059937 | MS.gene06921 | 0.808892 | 2.48E-50 | -1.69E-46 |
| MS.gene060167 | MS.gene06921 | 0.814352 | 1.61E-51 | -1.69E-46 |
| MS.gene060322 | MS.gene06921 | 0.837125 | 6.25E-57 | -1.69E-46 |
| MS.gene060572 | MS.gene06921 | 0.820942 | 5.25E-53 | -1.69E-46 |
| MS.gene060683 | MS.gene06921 | 0.813776 | 2.16E-51 | -1.69E-46 |
| MS.gene060685 | MS.gene06921 | 0.817745 | 2.81E-52 | -1.69E-46 |
| MS.gene060802 | MS.gene06921 | 0.819897 | 9.13E-53 | -1.69E-46 |
| MS.gene060803 | MS.gene06921 | 0.870847 | 1.07E-66 | -1.69E-46 |
| MS.gene060998 | MS.gene06921 | 0.838917 | 2.16E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06921.t1 | MTR_3g491560 | 97.464 | 276 | 7 | 0 | 1 | 276 | 56 | 331 | 0.0 | 555 |
| MS.gene06921.t1 | MTR_7g117665 | 57.818 | 275 | 113 | 2 | 2 | 276 | 43 | 314 | 9.90e-115 | 335 |
| MS.gene06921.t1 | MTR_7g117665 | 54.671 | 289 | 114 | 3 | 2 | 276 | 43 | 328 | 6.81e-110 | 323 |
| MS.gene06921.t1 | MTR_1g007990 | 40.580 | 276 | 152 | 5 | 2 | 276 | 652 | 916 | 1.44e-59 | 204 |
| MS.gene06921.t1 | MTR_1g008050 | 45.259 | 232 | 118 | 5 | 46 | 276 | 1151 | 1374 | 1.70e-58 | 201 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06921.t1 | AT3G49250 | 61.818 | 275 | 103 | 1 | 2 | 276 | 66 | 338 | 1.41e-120 | 351 |
| MS.gene06921.t1 | AT5G24280 | 41.778 | 225 | 120 | 3 | 50 | 272 | 1279 | 1494 | 5.59e-46 | 165 |
| MS.gene06921.t1 | AT5G24280 | 41.778 | 225 | 120 | 3 | 50 | 272 | 1279 | 1494 | 5.59e-46 | 165 |
| MS.gene06921.t1 | AT5G24280 | 41.778 | 225 | 120 | 3 | 50 | 272 | 1311 | 1526 | 6.86e-46 | 165 |
| MS.gene06921.t1 | AT5G24275 | 34.034 | 238 | 128 | 4 | 7 | 242 | 220 | 430 | 8.75e-35 | 130 |
| MS.gene06921.t1 | AT5G24275 | 34.034 | 238 | 128 | 4 | 7 | 242 | 220 | 430 | 8.75e-35 | 130 |
| MS.gene06921.t1 | AT5G24275 | 34.034 | 238 | 128 | 4 | 7 | 242 | 392 | 602 | 5.73e-34 | 130 |
| MS.gene06921.t1 | AT5G24275 | 34.034 | 238 | 128 | 4 | 7 | 242 | 597 | 807 | 1.22e-33 | 129 |
Find 67 sgRNAs with CRISPR-Local
Find 113 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGTGTCCGGCTGGATTTAT+TGG | 0.224477 | 3.1:-73504694 | MS.gene06921:CDS |
| TTCTTATTCATAGTTGCTAT+TGG | 0.233472 | 3.1:-73505503 | MS.gene06921:intron |
| CACTCCCCATTGGGCAATCT+TGG | 0.257269 | 3.1:+73504712 | None:intergenic |
| GAAGAATGGCGTCATCTAAT+TGG | 0.281502 | 3.1:+73506077 | None:intergenic |
| GATGGAGCTCTTTCTTTAGA+TGG | 0.317587 | 3.1:-73504514 | MS.gene06921:CDS |
| AGTGTCCGGCTGGATTTATT+GGG | 0.317673 | 3.1:-73504693 | MS.gene06921:CDS |
| TGTCTAACGCCAAGTGGTTA+TGG | 0.330066 | 3.1:-73504628 | MS.gene06921:CDS |
| CTTTAGATGGAGGAATGATT+AGG | 0.334200 | 3.1:-73504501 | MS.gene06921:CDS |
| TCTTATTCATAGTTGCTATT+GGG | 0.337983 | 3.1:-73505502 | MS.gene06921:intron |
| TGCCTCTATTGGAAGGCCTT+TGG | 0.344800 | 3.1:-73504917 | MS.gene06921:CDS |
| ATCGTCCGCATTACCAATCT+TGG | 0.394765 | 3.1:+73505457 | None:intergenic |
| TGATATGTCTTGAATCTCTA+AGG | 0.396970 | 3.1:-73504879 | MS.gene06921:intron |
| AGCTCTTGAATTGTATGATA+AGG | 0.402676 | 3.1:-73504980 | MS.gene06921:CDS |
| TAAATCCAAGATTGCCCAAT+GGG | 0.422999 | 3.1:-73504717 | MS.gene06921:CDS |
| AATAAAAGCTGTGGCCTTCA+CGG | 0.424469 | 3.1:-73504946 | MS.gene06921:CDS |
| CACGGGCTAGGTGCCTCTAT+TGG | 0.437611 | 3.1:-73504928 | MS.gene06921:CDS |
| GCGTTAGACAAAATAGGTTC+CGG | 0.439400 | 3.1:+73504641 | None:intergenic |
| ATTGTTTGTAGAACTTATGA+AGG | 0.448075 | 3.1:-73505009 | MS.gene06921:CDS |
| CTCCATGATGAGTCTTCACC+TGG | 0.448193 | 3.1:+73505360 | None:intergenic |
| CACTTGGCGTTAGACAAAAT+AGG | 0.453101 | 3.1:+73504635 | None:intergenic |
| ATTGCCCAATGGGGAGTGTC+CGG | 0.453427 | 3.1:-73504707 | MS.gene06921:CDS |
| TTTACCTTGCAAATGAAGAA+TGG | 0.453864 | 3.1:+73506063 | None:intergenic |
| CCCAATGGGGAGTGTCCGGC+TGG | 0.461596 | 3.1:-73504703 | MS.gene06921:CDS |
| TGCTGCTGGTATTCTGTGCC+AGG | 0.462317 | 3.1:-73505378 | MS.gene06921:CDS |
| GTTGGTGTTGTTGCTATGCT+AGG | 0.470088 | 3.1:-73505302 | MS.gene06921:CDS |
| ATCCAAAGGCCTTCCAATAG+AGG | 0.471825 | 3.1:+73504915 | None:intergenic |
| TCTTCAAGTACAACCAAGAT+TGG | 0.476782 | 3.1:-73505470 | MS.gene06921:CDS |
| ATAAAAGCTGTGGCCTTCAC+GGG | 0.484744 | 3.1:-73504945 | MS.gene06921:CDS |
| ACCACATATTTACCAGCATA+AGG | 0.498675 | 3.1:+73504769 | None:intergenic |
| TGATGACTCACAACGAAAGT+TGG | 0.501223 | 3.1:-73504746 | MS.gene06921:CDS |
| CCAGCCGGACACTCCCCATT+GGG | 0.503345 | 3.1:+73504703 | None:intergenic |
| TCCAGCCGGACACTCCCCAT+TGG | 0.514253 | 3.1:+73504702 | None:intergenic |
| GCACTAACACAGGATGTTGT+TGG | 0.516879 | 3.1:-73505320 | MS.gene06921:CDS |
| AGCTGTGGCCTTCACGGGCT+AGG | 0.525342 | 3.1:-73504940 | MS.gene06921:CDS |
| ATATGATTAATATAGACAAC+CGG | 0.533417 | 3.1:-73504660 | MS.gene06921:CDS |
| TCTATTGGAAGGCCTTTGGA+TGG | 0.556581 | 3.1:-73504913 | MS.gene06921:CDS |
| GACGCCATTCTTCATTTGCA+AGG | 0.557760 | 3.1:-73506067 | MS.gene06921:intron |
| TTGAGGATGACAATCTTAGC+AGG | 0.559697 | 3.1:-73505274 | MS.gene06921:intron |
| GCCTTATGCTGGTAAATATG+TGG | 0.560207 | 3.1:-73504770 | MS.gene06921:CDS |
| CCTCCTCCTCAGTTGTAATG+TGG | 0.565780 | 3.1:+73505432 | None:intergenic |
| GAAGACTCATCATGGAGCAC+AGG | 0.568029 | 3.1:-73505354 | MS.gene06921:CDS |
| TGCCAGGTGAAGACTCATCA+TGG | 0.570153 | 3.1:-73505362 | MS.gene06921:CDS |
| CCACATTACAACTGAGGAGG+AGG | 0.570572 | 3.1:-73505432 | MS.gene06921:CDS |
| GGCTAGGTGCCTCTATTGGA+AGG | 0.572742 | 3.1:-73504924 | MS.gene06921:CDS |
| GGAGCTCTTTCTTTAGATGG+AGG | 0.577727 | 3.1:-73504511 | MS.gene06921:CDS |
| TCTCTGAGGCCATAACCACT+TGG | 0.580160 | 3.1:+73504619 | None:intergenic |
| GCACTTCCTTGCATAAGTGA+TGG | 0.582385 | 3.1:-73504532 | MS.gene06921:CDS |
| TTCTCTTCTTGCACTAACAC+AGG | 0.587839 | 3.1:-73505330 | MS.gene06921:CDS |
| CTTGAATTGTATGATAAGGA+AGG | 0.590543 | 3.1:-73504976 | MS.gene06921:CDS |
| TCTTGGAGTGGAGACTATGT+TGG | 0.599213 | 3.1:-73505034 | MS.gene06921:CDS |
| TTTCTCGGAGTATCTTGGAG+TGG | 0.601969 | 3.1:-73505046 | MS.gene06921:CDS |
| TACAACCAAGATTGGTAATG+CGG | 0.624832 | 3.1:-73505462 | MS.gene06921:CDS |
| GCAAACCCAATAAATCCAGC+CGG | 0.628940 | 3.1:+73504688 | None:intergenic |
| GTCTACAAGTATACAAGACA+AGG | 0.633194 | 3.1:-73504570 | MS.gene06921:CDS |
| TAGAGGCACCTAGCCCGTGA+AGG | 0.634434 | 3.1:+73504932 | None:intergenic |
| GGAGGAATGATTAGGAGTTG+TGG | 0.637606 | 3.1:-73504493 | MS.gene06921:CDS |
| AGAGCTCCATCACTTATGCA+AGG | 0.645316 | 3.1:+73504526 | None:intergenic |
| ACTAGAAATCGACCATCCAA+AGG | 0.646590 | 3.1:+73504901 | None:intergenic |
| GGCAGCATAAATAAAAGCTG+TGG | 0.651617 | 3.1:-73504955 | MS.gene06921:CDS |
| TGCTATGCTAGGAAAAGTTG+AGG | 0.657021 | 3.1:-73505291 | MS.gene06921:CDS |
| TTAAATCCAAGATTGCCCAA+TGG | 0.666860 | 3.1:-73504718 | MS.gene06921:CDS |
| TCTCCACATTACAACTGAGG+AGG | 0.672917 | 3.1:-73505435 | MS.gene06921:CDS |
| GATGAAAATTAAGCAGCATG+AGG | 0.674683 | 3.1:-73506131 | MS.gene06921:CDS |
| CGATCTCCACATTACAACTG+AGG | 0.681013 | 3.1:-73505438 | MS.gene06921:CDS |
| TATAAAACAGAGTTTCTCTG+AGG | 0.681314 | 3.1:+73504605 | None:intergenic |
| AAATCCAAGATTGCCCAATG+GGG | 0.722987 | 3.1:-73504716 | MS.gene06921:CDS |
| TCTACAAGTATACAAGACAA+GGG | 0.738588 | 3.1:-73504569 | MS.gene06921:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGTATAGATTATTTTCTTTT+TGG | - | chr3.1:73505365-73505384 | MS.gene06921:CDS | 15.0% |
| !! | ATATGATTAATATAGACAAC+CGG | - | chr3.1:73505949-73505968 | MS.gene06921:intron | 20.0% |
| !!! | ATGTTTTTGTCTTTTATTTG+TGG | - | chr3.1:73505769-73505788 | MS.gene06921:intron | 20.0% |
| !!! | TTTTGATTATGAAGTTTATG+CGG | - | chr3.1:73504746-73504765 | MS.gene06921:CDS | 20.0% |
| ! | ATAGCAACTATGAATAAGAA+AGG | + | chr3.1:73505106-73505125 | None:intergenic | 25.0% |
| ! | ATTGTTTGTAGAACTTATGA+AGG | - | chr3.1:73505600-73505619 | MS.gene06921:intron | 25.0% |
| ! | TAATATGTTTCCGTATGTAT+AGG | - | chr3.1:73504891-73504910 | MS.gene06921:CDS | 25.0% |
| ! | TGAATAATTCTTACAAGACT+GGG | - | chr3.1:73504796-73504815 | MS.gene06921:intron | 25.0% |
| ! | TTCCTTAAAAAAATGAAGAG+CGG | + | chr3.1:73504984-73505003 | None:intergenic | 25.0% |
| ! | TTCTTATTCATAGTTGCTAT+TGG | - | chr3.1:73505106-73505125 | MS.gene06921:intron | 25.0% |
| ! | TTGAATAATTCTTACAAGAC+TGG | - | chr3.1:73504795-73504814 | MS.gene06921:intron | 25.0% |
| !! | TAATAGGTACTTTGTTTCTT+CGG | - | chr3.1:73505421-73505440 | MS.gene06921:CDS | 25.0% |
| !! | TCTTATTCATAGTTGCTATT+GGG | - | chr3.1:73505107-73505126 | MS.gene06921:intron | 25.0% |
| !! | TTATTTCACAGTCTTGTAAT+AGG | - | chr3.1:73505405-73505424 | MS.gene06921:CDS | 25.0% |
| !!! | CTTTTTTCCTGTTTTGTTTT+AGG | - | chr3.1:73505817-73505836 | MS.gene06921:intron | 25.0% |
| AATAAGTCGAGTGAAACTAT+CGG | + | chr3.1:73505530-73505549 | None:intergenic | 30.0% | |
| AGCTCTTGAATTGTATGATA+AGG | - | chr3.1:73505629-73505648 | MS.gene06921:intron | 30.0% | |
| ATAGTTTCACTCGACTTATT+TGG | - | chr3.1:73505530-73505549 | MS.gene06921:intron | 30.0% | |
| ATTATTCAATCTCTTGCTGA+TGG | + | chr3.1:73504784-73504803 | None:intergenic | 30.0% | |
| ATTTCTCTATCAACAAACGT+AGG | - | chr3.1:73505033-73505052 | MS.gene06921:CDS | 30.0% | |
| CTTGAATTGTATGATAAGGA+AGG | - | chr3.1:73505633-73505652 | MS.gene06921:intron | 30.0% | |
| TATAAAACAGAGTTTCTCTG+AGG | + | chr3.1:73506007-73506026 | None:intergenic | 30.0% | |
| TCTACAAGTATACAAGACAA+GGG | - | chr3.1:73506040-73506059 | MS.gene06921:intron | 30.0% | |
| TGATATGTCTTGAATCTCTA+AGG | - | chr3.1:73505730-73505749 | MS.gene06921:intron | 30.0% | |
| TTAAGCTTTCTTGATTCTGA+AGG | - | chr3.1:73504687-73504706 | MS.gene06921:CDS | 30.0% | |
| TTATTCAATCTCTTGCTGAT+GGG | + | chr3.1:73504783-73504802 | None:intergenic | 30.0% | |
| TTTACCTTGCAAATGAAGAA+TGG | + | chr3.1:73504549-73504568 | None:intergenic | 30.0% | |
| ! | GATGACTTTTCTTCTTTGTT+TGG | - | chr3.1:73504587-73504606 | MS.gene06921:CDS | 30.0% |
| !! | TTGTCTTTTATTTGTGGTTG+TGG | - | chr3.1:73505775-73505794 | MS.gene06921:intron | 30.0% |
| ACCACATATTTACCAGCATA+AGG | + | chr3.1:73505843-73505862 | None:intergenic | 35.0% | |
| CATAAGGCCTAAAACAAAAC+AGG | + | chr3.1:73505827-73505846 | None:intergenic | 35.0% | |
| CTTTAGATGGAGGAATGATT+AGG | - | chr3.1:73506108-73506127 | MS.gene06921:CDS | 35.0% | |
| GAGTTCATTCACTGAATCAA+TGG | - | chr3.1:73504663-73504682 | MS.gene06921:CDS | 35.0% | |
| GTCTACAAGTATACAAGACA+AGG | - | chr3.1:73506039-73506058 | MS.gene06921:intron | 35.0% | |
| GTGAATGAACTCTACAGAAT+CGG | + | chr3.1:73504655-73504674 | None:intergenic | 35.0% | |
| TAAATCCAAGATTGCCCAAT+GGG | - | chr3.1:73505892-73505911 | MS.gene06921:intron | 35.0% | |
| TATTCAATCTCTTGCTGATG+GGG | + | chr3.1:73504782-73504801 | None:intergenic | 35.0% | |
| TCTTCAAGTACAACCAAGAT+TGG | - | chr3.1:73505139-73505158 | MS.gene06921:intron | 35.0% | |
| TTAAATCCAAGATTGCCCAA+TGG | - | chr3.1:73505891-73505910 | MS.gene06921:intron | 35.0% | |
| !! | TACAACCAAGATTGGTAATG+CGG | - | chr3.1:73505147-73505166 | MS.gene06921:intron | 35.0% |
| !! | TTTGGAATGCAGACTTTTCT+CGG | - | chr3.1:73505548-73505567 | MS.gene06921:intron | 35.0% |
| !!! | TGCCGCTCTTCATTTTTTTA+AGG | - | chr3.1:73504979-73504998 | MS.gene06921:CDS | 35.0% |
| !!! | TTTTGTTTTAGGCCTTATGC+TGG | - | chr3.1:73505828-73505847 | MS.gene06921:intron | 35.0% |
| AAATCCAAGATTGCCCAATG+GGG | - | chr3.1:73505893-73505912 | MS.gene06921:intron | 40.0% | |
| AATAAAAGCTGTGGCCTTCA+CGG | - | chr3.1:73505663-73505682 | MS.gene06921:intron | 40.0% | |
| AATGCCCCATCTAAAAACTC+TGG | + | chr3.1:73504869-73504888 | None:intergenic | 40.0% | |
| ACTAGAAATCGACCATCCAA+AGG | + | chr3.1:73505711-73505730 | None:intergenic | 40.0% | |
| ATGCCCCATCTAAAAACTCT+GGG | + | chr3.1:73504868-73504887 | None:intergenic | 40.0% | |
| ATTCAATCTCTTGCTGATGG+GGG | + | chr3.1:73504781-73504800 | None:intergenic | 40.0% | |
| CAAGACTGTGAAATAAGTGC+AGG | + | chr3.1:73505401-73505420 | None:intergenic | 40.0% | |
| CACTTGGCGTTAGACAAAAT+AGG | + | chr3.1:73505977-73505996 | None:intergenic | 40.0% | |
| CCGTATGTATAGGCTGAAAA+GGG | - | chr3.1:73504901-73504920 | MS.gene06921:CDS | 40.0% | |
| GAAGAATGGCGTCATCTAAT+TGG | + | chr3.1:73504535-73504554 | None:intergenic | 40.0% | |
| GATGGAGCTCTTTCTTTAGA+TGG | - | chr3.1:73506095-73506114 | MS.gene06921:CDS | 40.0% | |
| GCCTTATGCTGGTAAATATG+TGG | - | chr3.1:73505839-73505858 | MS.gene06921:intron | 40.0% | |
| GCGTTAGACAAAATAGGTTC+CGG | + | chr3.1:73505971-73505990 | None:intergenic | 40.0% | |
| GGCAGCATAAATAAAAGCTG+TGG | - | chr3.1:73505654-73505673 | MS.gene06921:intron | 40.0% | |
| TCCGTATGTATAGGCTGAAA+AGG | - | chr3.1:73504900-73504919 | MS.gene06921:CDS | 40.0% | |
| TGATGACTCACAACGAAAGT+TGG | - | chr3.1:73505863-73505882 | MS.gene06921:intron | 40.0% | |
| TGCTATGCTAGGAAAAGTTG+AGG | - | chr3.1:73505318-73505337 | MS.gene06921:CDS | 40.0% | |
| TTCTCTTCTTGCACTAACAC+AGG | - | chr3.1:73505279-73505298 | MS.gene06921:CDS | 40.0% | |
| TTGAGGATGACAATCTTAGC+AGG | - | chr3.1:73505335-73505354 | MS.gene06921:CDS | 40.0% | |
| ! | CCCTTTTCAGCCTATACATA+CGG | + | chr3.1:73504904-73504923 | None:intergenic | 40.0% |
| ! | CTATTTTGTCTAACGCCAAG+TGG | - | chr3.1:73505975-73505994 | MS.gene06921:intron | 40.0% |
| ! | GAGTTGTGGTGTATTTTCCT+TGG | - | chr3.1:73506130-73506149 | MS.gene06921:CDS | 40.0% |
| !! | AGACTTTTCTCGGAGTATCT+TGG | - | chr3.1:73505558-73505577 | MS.gene06921:intron | 40.0% |
| !! | CTGACCCAGAGTTTTTAGAT+GGG | - | chr3.1:73504861-73504880 | MS.gene06921:intron | 40.0% |
| !! | TGACCCAGAGTTTTTAGATG+GGG | - | chr3.1:73504862-73504881 | MS.gene06921:intron | 40.0% |
| !! | TTAGCTTTGGAGGTTGAGAT+TGG | - | chr3.1:73504838-73504857 | MS.gene06921:intron | 40.0% |
| ACCTCCAAAGCTAACTCAAG+CGG | + | chr3.1:73504832-73504851 | None:intergenic | 45.0% | |
| AGAGCTCCATCACTTATGCA+AGG | + | chr3.1:73506086-73506105 | None:intergenic | 45.0% | |
| AGTGTCCGGCTGGATTTATT+GGG | - | chr3.1:73505916-73505935 | MS.gene06921:intron | 45.0% | |
| ATAAAAGCTGTGGCCTTCAC+GGG | - | chr3.1:73505664-73505683 | MS.gene06921:intron | 45.0% | |
| ATCCAAAGGCCTTCCAATAG+AGG | + | chr3.1:73505697-73505716 | None:intergenic | 45.0% | |
| ATCGTCCGCATTACCAATCT+TGG | + | chr3.1:73505155-73505174 | None:intergenic | 45.0% | |
| CAGCATGAAAAATCTGCTGC+TGG | - | chr3.1:73505217-73505236 | MS.gene06921:intron | 45.0% | |
| CGATCTCCACATTACAACTG+AGG | - | chr3.1:73505171-73505190 | MS.gene06921:intron | 45.0% | |
| GAAGGCTGCACGAGTATTAT+CGG | - | chr3.1:73504705-73504724 | MS.gene06921:CDS | 45.0% | |
| GACGCCATTCTTCATTTGCA+AGG | - | chr3.1:73504542-73504561 | MS.gene06921:CDS | 45.0% | |
| GCAAACCCAATAAATCCAGC+CGG | + | chr3.1:73505924-73505943 | None:intergenic | 45.0% | |
| GCACTAACACAGGATGTTGT+TGG | - | chr3.1:73505289-73505308 | MS.gene06921:CDS | 45.0% | |
| GCACTTCCTTGCATAAGTGA+TGG | - | chr3.1:73506077-73506096 | MS.gene06921:CDS | 45.0% | |
| GGAGCTCTTTCTTTAGATGG+AGG | - | chr3.1:73506098-73506117 | MS.gene06921:CDS | 45.0% | |
| GGAGGAATGATTAGGAGTTG+TGG | - | chr3.1:73506116-73506135 | MS.gene06921:CDS | 45.0% | |
| TCTATTGGAAGGCCTTTGGA+TGG | - | chr3.1:73505696-73505715 | MS.gene06921:intron | 45.0% | |
| TCTCCACATTACAACTGAGG+AGG | - | chr3.1:73505174-73505193 | MS.gene06921:intron | 45.0% | |
| TCTTGGAGTGGAGACTATGT+TGG | - | chr3.1:73505575-73505594 | MS.gene06921:intron | 45.0% | |
| TGTCTAACGCCAAGTGGTTA+TGG | - | chr3.1:73505981-73506000 | MS.gene06921:intron | 45.0% | |
| ! | TTTCTCGGAGTATCTTGGAG+TGG | - | chr3.1:73505563-73505582 | MS.gene06921:intron | 45.0% |
| !! | GCTGACCCAGAGTTTTTAGA+TGG | - | chr3.1:73504860-73504879 | MS.gene06921:intron | 45.0% |
| !! | GTTGGTGTTGTTGCTATGCT+AGG | - | chr3.1:73505307-73505326 | MS.gene06921:CDS | 45.0% |
| CAAAGCTAACTCAAGCGGTG+AGG | + | chr3.1:73504827-73504846 | None:intergenic | 50.0% | |
| CCACATTACAACTGAGGAGG+AGG | - | chr3.1:73505177-73505196 | MS.gene06921:intron | 50.0% | |
| CCTCCTCCTCAGTTGTAATG+TGG | + | chr3.1:73505180-73505199 | None:intergenic | 50.0% | |
| CTAACTCAAGCGGTGAGGAT+TGG | + | chr3.1:73504822-73504841 | None:intergenic | 50.0% | |
| CTCCATGATGAGTCTTCACC+TGG | + | chr3.1:73505252-73505271 | None:intergenic | 50.0% | |
| GAAGACTCATCATGGAGCAC+AGG | - | chr3.1:73505255-73505274 | MS.gene06921:intron | 50.0% | |
| GAGTGTCCGGCTGGATTTAT+TGG | - | chr3.1:73505915-73505934 | MS.gene06921:intron | 50.0% | |
| TGCCAGGTGAAGACTCATCA+TGG | - | chr3.1:73505247-73505266 | MS.gene06921:intron | 50.0% | |
| TGCCTCTATTGGAAGGCCTT+TGG | - | chr3.1:73505692-73505711 | MS.gene06921:intron | 50.0% | |
| ! | CTCACCGCTTGAGTTAGCTT+TGG | - | chr3.1:73504825-73504844 | MS.gene06921:intron | 50.0% |
| !! | ACCGCTTGAGTTAGCTTTGG+AGG | - | chr3.1:73504828-73504847 | MS.gene06921:intron | 50.0% |
| !! | TCTCTGAGGCCATAACCACT+TGG | + | chr3.1:73505993-73506012 | None:intergenic | 50.0% |
| CACTCCCCATTGGGCAATCT+TGG | + | chr3.1:73505900-73505919 | None:intergenic | 55.0% | |
| GGCTAGGTGCCTCTATTGGA+AGG | - | chr3.1:73505685-73505704 | MS.gene06921:intron | 55.0% | |
| TGCTGCTGGTATTCTGTGCC+AGG | - | chr3.1:73505231-73505250 | MS.gene06921:intron | 55.0% | |
| !! | ATTGCCCAATGGGGAGTGTC+CGG | - | chr3.1:73505902-73505921 | MS.gene06921:intron | 55.0% |
| !! | CACGGGCTAGGTGCCTCTAT+TGG | - | chr3.1:73505681-73505700 | MS.gene06921:intron | 60.0% |
| !! | TAGAGGCACCTAGCCCGTGA+AGG | + | chr3.1:73505680-73505699 | None:intergenic | 60.0% |
| AGCTGTGGCCTTCACGGGCT+AGG | - | chr3.1:73505669-73505688 | MS.gene06921:intron | 65.0% | |
| CCAGCCGGACACTCCCCATT+GGG | + | chr3.1:73505909-73505928 | None:intergenic | 65.0% | |
| TCCAGCCGGACACTCCCCAT+TGG | + | chr3.1:73505910-73505929 | None:intergenic | 65.0% | |
| !! | CCCAATGGGGAGTGTCCGGC+TGG | - | chr3.1:73505906-73505925 | MS.gene06921:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 73504479 | 73506152 | 73504479 | ID=MS.gene06921 |
| chr3.1 | mRNA | 73504479 | 73506152 | 73504479 | ID=MS.gene06921.t1;Parent=MS.gene06921 |
| chr3.1 | exon | 73506068 | 73506152 | 73506068 | ID=MS.gene06921.t1.exon1;Parent=MS.gene06921.t1 |
| chr3.1 | CDS | 73506068 | 73506152 | 73506068 | ID=cds.MS.gene06921.t1;Parent=MS.gene06921.t1 |
| chr3.1 | exon | 73505275 | 73505512 | 73505275 | ID=MS.gene06921.t1.exon2;Parent=MS.gene06921.t1 |
| chr3.1 | CDS | 73505275 | 73505512 | 73505275 | ID=cds.MS.gene06921.t1;Parent=MS.gene06921.t1 |
| chr3.1 | exon | 73504880 | 73505071 | 73504880 | ID=MS.gene06921.t1.exon3;Parent=MS.gene06921.t1 |
| chr3.1 | CDS | 73504880 | 73505071 | 73504880 | ID=cds.MS.gene06921.t1;Parent=MS.gene06921.t1 |
| chr3.1 | exon | 73504479 | 73504792 | 73504479 | ID=MS.gene06921.t1.exon4;Parent=MS.gene06921.t1 |
| chr3.1 | CDS | 73504479 | 73504792 | 73504479 | ID=cds.MS.gene06921.t1;Parent=MS.gene06921.t1 |
| Gene Sequence |
| Protein sequence |
