AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22454


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22454.t1 MTR_7g100860 86.022 93 5 4 1 89 1 89 4.95e-49 151
MS.gene22454.t1 MTR_1g066720 75.676 37 8 1 23 58 18 54 1.46e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22454.t1 AT5G03120 43.678 87 42 2 16 102 10 89 4.86e-17 70.9
MS.gene22454.t1 AT5G03120 47.692 65 33 1 16 80 10 73 4.23e-16 67.4

Find 24 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACAAACCTCTAGAGAAATA+TGG 0.278570 7.1:-8353004 None:intergenic
AGCATCAACACCCGTAGCTT+TGG 0.412256 7.1:-8353484 None:intergenic
TCATTATCATCACAGGATCT+AGG 0.434501 7.1:+8353547 MS.gene22454:CDS
ACAAACCTCTAGAGAAATAT+GGG 0.461398 7.1:-8353003 None:intergenic
AAGAAAGAGAAAGAAACAAT+TGG 0.485064 7.1:-8352969 None:intergenic
GAAACAATTGGCGATGAAGA+TGG 0.489266 7.1:-8352957 None:intergenic
GGCACAAACGAGTGAGTCTC+AGG 0.492086 7.1:-8353396 None:intergenic
TTTAGGTGGATCGGAAGCAT+TGG 0.492339 7.1:+8353341 MS.gene22454:intron
AAATTTGTGCATGTCATACA+TGG 0.499981 7.1:+8352922 None:intergenic
ATTTATGAGATCGATTATAG+AGG 0.512251 7.1:+8353372 MS.gene22454:CDS
GGCTTACCATGGGAATGATC+AGG 0.514926 7.1:-8353423 None:intergenic
TGCAGCTGCAACCAAAGCTA+CGG 0.523815 7.1:+8353473 MS.gene22454:CDS
TGATGCTACCATGAAACAAA+AGG 0.546319 7.1:+8353500 MS.gene22454:CDS
CTCTTGTCATGAGCTGAATG+AGG 0.554978 7.1:-8353444 None:intergenic
CAACTACTCATTATCATCAC+AGG 0.565467 7.1:+8353540 MS.gene22454:CDS
ACTCTCCCATATTTCTCTAG+AGG 0.566327 7.1:+8352998 MS.gene22454:CDS
GAGCTGAATGAGGCTTACCA+TGG 0.584782 7.1:-8353434 None:intergenic
GCAGCTGCAACCAAAGCTAC+GGG 0.602211 7.1:+8353474 MS.gene22454:CDS
CCATGGGAATGATCAGGTGG+AGG 0.610610 7.1:-8353417 None:intergenic
CCTCCACCTGATCATTCCCA+TGG 0.611138 7.1:+8353417 MS.gene22454:CDS
ACAATTGGCGATGAAGATGG+AGG 0.611543 7.1:-8352954 None:intergenic
AGCTGAATGAGGCTTACCAT+GGG 0.626115 7.1:-8353433 None:intergenic
CAATTGGCGATGAAGATGGA+GGG 0.648695 7.1:-8352953 None:intergenic
TTACCATGGGAATGATCAGG+TGG 0.701175 7.1:-8353420 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATTGTCTATTGTAAAT+AGG - chr7.1:8353175-8353194 None:intergenic 15.0%
!! TATTATAAAATTTATGAGAG+TGG - chr7.1:8353063-8353082 None:intergenic 15.0%
!!! GTGTTATGTTTTTAAATTTT+AGG + chr7.1:8353324-8353343 MS.gene22454:intron 15.0%
!!! TTATGTTTTTAAATTTTAGG+TGG + chr7.1:8353327-8353346 MS.gene22454:intron 15.0%
!! GAGTAATATTGTAGAAAAAA+AGG - chr7.1:8353144-8353163 None:intergenic 20.0%
!!! TTTTTAAATTTTAGGTGGAT+CGG + chr7.1:8353332-8353351 MS.gene22454:intron 20.0%
! AAGAAAGAGAAAGAAACAAT+TGG - chr7.1:8352972-8352991 None:intergenic 25.0%
! ATTTATGAGATCGATTATAG+AGG + chr7.1:8353372-8353391 MS.gene22454:CDS 25.0%
! TATAAAATTTATGAGAGTGG+TGG - chr7.1:8353060-8353079 None:intergenic 25.0%
CTTTCTCTTGTAAATAGGAT+CGG + chr7.1:8353278-8353297 MS.gene22454:intron 30.0%
GAAAGAGGGTTTGTAAAATT+CGG - chr7.1:8353031-8353050 None:intergenic 30.0%
GTCAACTTTCTCTTGTAAAT+AGG + chr7.1:8353273-8353292 MS.gene22454:intron 30.0%
TTGTCTATTGTAAATAGGAC+CGG - chr7.1:8353170-8353189 None:intergenic 30.0%
! ACAAACCTCTAGAGAAATAT+GGG - chr7.1:8353006-8353025 None:intergenic 30.0%
!! AAAACTTGCCTTTTGTTTCA+TGG - chr7.1:8353511-8353530 None:intergenic 30.0%
!! GGGAGTATTAATTTGTTTCT+TGG + chr7.1:8353302-8353321 MS.gene22454:intron 30.0%
CAACTACTCATTATCATCAC+AGG + chr7.1:8353540-8353559 MS.gene22454:CDS 35.0%
TCATTATCATCACAGGATCT+AGG + chr7.1:8353547-8353566 MS.gene22454:CDS 35.0%
TGATGCTACCATGAAACAAA+AGG + chr7.1:8353500-8353519 MS.gene22454:CDS 35.0%
TTCTACAATATTACTCCCTC+CGG + chr7.1:8353148-8353167 MS.gene22454:intron 35.0%
! GACAAACCTCTAGAGAAATA+TGG - chr7.1:8353007-8353026 None:intergenic 35.0%
! TTTATGAGAGTGGTGGAAAT+TGG - chr7.1:8353053-8353072 None:intergenic 35.0%
ACTCTCCCATATTTCTCTAG+AGG + chr7.1:8352998-8353017 MS.gene22454:CDS 40.0%
CTATTGTAAATAGGACCGGA+GGG - chr7.1:8353166-8353185 None:intergenic 40.0%
CTCTTGTAAATAGGATCGGA+GGG + chr7.1:8353282-8353301 MS.gene22454:intron 40.0%
GAAACAATTGGCGATGAAGA+TGG - chr7.1:8352960-8352979 None:intergenic 40.0%
TCTATTGTAAATAGGACCGG+AGG - chr7.1:8353167-8353186 None:intergenic 40.0%
TCTCTTGTAAATAGGATCGG+AGG + chr7.1:8353281-8353300 MS.gene22454:intron 40.0%
! AGTGGTGGAAATTGGAAAGA+GGG - chr7.1:8353045-8353064 None:intergenic 40.0%
ACAATTGGCGATGAAGATGG+AGG - chr7.1:8352957-8352976 None:intergenic 45.0%
AGCTGAATGAGGCTTACCAT+GGG - chr7.1:8353436-8353455 None:intergenic 45.0%
CAATTGGCGATGAAGATGGA+GGG - chr7.1:8352956-8352975 None:intergenic 45.0%
CTCTTGTCATGAGCTGAATG+AGG - chr7.1:8353447-8353466 None:intergenic 45.0%
TTACCATGGGAATGATCAGG+TGG - chr7.1:8353423-8353442 None:intergenic 45.0%
! GAGTGGTGGAAATTGGAAAG+AGG - chr7.1:8353046-8353065 None:intergenic 45.0%
! TTTAGGTGGATCGGAAGCAT+TGG + chr7.1:8353341-8353360 MS.gene22454:intron 45.0%
AGCATCAACACCCGTAGCTT+TGG - chr7.1:8353487-8353506 None:intergenic 50.0%
GAGCTGAATGAGGCTTACCA+TGG - chr7.1:8353437-8353456 None:intergenic 50.0%
GGCTTACCATGGGAATGATC+AGG - chr7.1:8353426-8353445 None:intergenic 50.0%
TGCAGCTGCAACCAAAGCTA+CGG + chr7.1:8353473-8353492 MS.gene22454:CDS 50.0%
CCATGGGAATGATCAGGTGG+AGG - chr7.1:8353420-8353439 None:intergenic 55.0%
CCTCCACCTGATCATTCCCA+TGG + chr7.1:8353417-8353436 MS.gene22454:CDS 55.0%
GCAGCTGCAACCAAAGCTAC+GGG + chr7.1:8353474-8353493 MS.gene22454:CDS 55.0%
GGCACAAACGAGTGAGTCTC+AGG - chr7.1:8353399-8353418 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 8352932 8353587 8352932 ID=MS.gene22454
chr7.1 mRNA 8352932 8353587 8352932 ID=MS.gene22454.t1;Parent=MS.gene22454
chr7.1 exon 8352932 8353019 8352932 ID=MS.gene22454.t1.exon1;Parent=MS.gene22454.t1
chr7.1 CDS 8352932 8353019 8352932 ID=cds.MS.gene22454.t1;Parent=MS.gene22454.t1
chr7.1 exon 8353346 8353587 8353346 ID=MS.gene22454.t1.exon2;Parent=MS.gene22454.t1
chr7.1 CDS 8353346 8353587 8353346 ID=cds.MS.gene22454.t1;Parent=MS.gene22454.t1
Gene Sequence

>MS.gene22454

ATGTCATACATGGATCTAAGACCCTCCATCTTCATCGCCAATTGTTTCTTTCTCTTTCTTATACTCACTCTCCCATATTTCTCTAGAGGTTTGTCACCGAATTTTACAAACCCTCTTTCCAATTTCCACCACTCTCATAAATTTTATAATATGTGTGTATGCTAATTTATATTATATTATTTGATTTATATAGTACACTATCATTATTACCTTTTTTTCTACAATATTACTCCCTCCGGTCCTATTTACAATAGACAATTAATTTTTTAGATACATTAAATAATTTATGTATTTAGTTAGATTCTTGACTAGATACATAAATTATTCAATGTATCTAAAAAGTCAACTTTCTCTTGTAAATAGGATCGGAGGGAGTATTAATTTGTTTCTTGGTGTTATGTTTTTAAATTTTAGGTGGATCGGAAGCATTGGATTCAGAAATTTATGAGATCGATTATAGAGGTCCTGAGACTCACTCGTTTGTGCCTCCACCTGATCATTCCCATGGTAAGCCTCATTCAGCTCATGACAAGAGCTCTACTGCAGCTGCAACCAAAGCTACGGGTGTTGATGCTACCATGAAACAAAAGGCAAGTTTTGAATTAAATCAACTACTCATTATCATCACAGGATCTAGGAAATTTTATTTCGTATAA

Protein sequence

>MS.gene22454.t1

MSYMDLRPSIFIANCFFLFLILTLPYFSRGGSEALDSEIYEIDYRGPETHSFVPPPDHSHGKPHSAHDKSSTAAATKATGVDATMKQKASFELNQLLIIITGSRKFYFV