AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33475


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33475.t1 MTR_4g015010 93.916 263 12 3 1 260 14 275 0.0 500
MS.gene33475.t1 MTR_7g067000 60.714 252 84 8 1 250 1 239 9.84e-84 251
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33475.t1 AT3G09050 52.263 243 101 5 16 249 7 243 4.04e-73 224

Find 38 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTCGTCTTACGGATTATT+TGG 0.131531 4.3:-83417350 MS.gene33475:CDS
ATTGGTGAAGATGCTCTTAA+AGG 0.254433 4.3:-83417268 MS.gene33475:CDS
AATGTTTACTTATTCTTCAT+TGG 0.294155 4.3:-83415956 MS.gene33475:CDS
TGGAAGAAAGTTGCAGTTCT+TGG 0.299282 4.3:-83415875 MS.gene33475:CDS
CCAGGTTGTGAAGTATTATC+AGG 0.334032 4.3:+83417430 None:intergenic
GTTGATAGAAGAAGGATAGA+AGG 0.335400 4.3:+83417540 None:intergenic
GTAACAACAAATGAGGAAAA+TGG 0.350272 4.3:-83415995 MS.gene33475:CDS
TCTGAATACTTGACACAGTT+TGG 0.366087 4.3:-83417319 MS.gene33475:CDS
GAGTGAGATGCTAGCATTTG+AGG 0.367845 4.3:-83416191 MS.gene33475:CDS
TTCTTCTTGTGTTGTGGTGG+GGG 0.383441 4.3:+83417494 None:intergenic
ATAGAAGGAAGGGTGAAATA+TGG 0.405812 4.3:+83417555 None:intergenic
CAAGTTGAGCTAGTAGCTCT+TGG 0.422521 4.3:+83417392 None:intergenic
ACTTCTTCTTGTGTTGTGGT+GGG 0.425298 4.3:+83417492 None:intergenic
CTTCTTCTTGTGTTGTGGTG+GGG 0.430419 4.3:+83417493 None:intergenic
AGTATTATCAGGTTCATCAT+TGG 0.435688 4.3:+83417441 None:intergenic
AATGGAGATTGAAGGACAAA+TGG 0.440803 4.3:-83416119 MS.gene33475:CDS
GGACTATTCTTTAAGAACTT+AGG 0.444508 4.3:-83416079 MS.gene33475:CDS
AACTTCTTCTTGTGTTGTGG+TGG 0.447722 4.3:+83417491 None:intergenic
CATCATTAAGTAGTACTGAT+TGG 0.456180 4.3:-83415895 MS.gene33475:CDS
ACTAGACAGAGTAGAGTTTG+AGG 0.456846 4.3:-83416158 MS.gene33475:CDS
TGGCTTTGAACATCACCTCC+AGG 0.458846 4.3:+83417412 None:intergenic
AAAGCTGAAATTGATAAGAT+TGG 0.462568 4.3:-83417286 MS.gene33475:CDS
TTTAAGAACTTAGGACAGAA+AGG 0.478730 4.3:-83416070 MS.gene33475:CDS
ATAGAAGAAGGATAGAAGGA+AGG 0.483138 4.3:+83417544 None:intergenic
ATCAGGTTCATCATTGGAGT+TGG 0.486180 4.3:+83417447 None:intergenic
CAGAACTTCTTCTTGTGTTG+TGG 0.493087 4.3:+83417488 None:intergenic
GGAAAGTGAAAGCAAAGTAA+TGG 0.500197 4.3:-83416137 MS.gene33475:CDS
TAGAAGAAGGATAGAAGGAA+GGG 0.500325 4.3:+83417545 None:intergenic
TGAAGATGCTCTTAAAGGAT+TGG 0.511035 4.3:-83417263 MS.gene33475:CDS
ATGGAAGTTGACAGAAATGA+AGG 0.525154 4.3:-83416100 MS.gene33475:CDS
GTGGTGGTGTTGATAGAAGA+AGG 0.543371 4.3:+83417532 None:intergenic
GAAAGATGTAACAACAAATG+AGG 0.557127 4.3:-83416002 MS.gene33475:CDS
AGCAAAGTAATGGAGATTGA+AGG 0.564863 4.3:-83416127 MS.gene33475:CDS
CCTGATAATACTTCACAACC+TGG 0.574595 4.3:-83417430 MS.gene33475:CDS
GGGGTGCAGAGTAGTAAAAG+TGG 0.581339 4.3:+83417513 None:intergenic
AGTTCTTGGAGCTATTCTTG+TGG 0.591041 4.3:-83415861 MS.gene33475:CDS
GTGCAGAGTAGTAAAAGTGG+TGG 0.674073 4.3:+83417516 None:intergenic
GATAATACTTCACAACCTGG+AGG 0.739356 4.3:-83417427 MS.gene33475:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATATCTTATAAAAAAATA+TGG + chr4.3:83416916-83416935 None:intergenic 10.0%
!!! ATACTTTAAAAACTGAATTA+TGG + chr4.3:83416254-83416273 None:intergenic 15.0%
!! AATCACAAATGAATGTAAAA+GGG + chr4.3:83417134-83417153 None:intergenic 20.0%
!! AATGTTTACTTATTCTTCAT+TGG - chr4.3:83417411-83417430 MS.gene33475:CDS 20.0%
!! TAATCACAAATGAATGTAAA+AGG + chr4.3:83417135-83417154 None:intergenic 20.0%
! AAAACTGAATTATGGTTTAC+TGG + chr4.3:83416246-83416265 None:intergenic 25.0%
! AAAGCTGAAATTGATAAGAT+TGG - chr4.3:83416081-83416100 MS.gene33475:CDS 25.0%
! TACTTTGTACACTTATCATA+TGG - chr4.3:83417027-83417046 MS.gene33475:intron 25.0%
! TTAAAGTATAGTTCCTACTA+GGG + chr4.3:83416355-83416374 None:intergenic 25.0%
!! CTTTTTACATATGGAATAGT+TGG - chr4.3:83417435-83417454 MS.gene33475:CDS 25.0%
!! TTTAAAGTATAGTTCCTACT+AGG + chr4.3:83416356-83416375 None:intergenic 25.0%
!!! CATCTTTTCTATTTCTTCTT+TGG + chr4.3:83417346-83417365 None:intergenic 25.0%
!!! TTCATTGGTCTTTTTACATA+TGG - chr4.3:83417426-83417445 MS.gene33475:CDS 25.0%
AAACTGTAGTTTGAATCCTA+GGG - chr4.3:83416471-83416490 MS.gene33475:intron 30.0%
AGATAAAATGAGAGAGAGAT+TGG - chr4.3:83416397-83416416 MS.gene33475:intron 30.0%
AGGAAAATGGAAGAAAAACA+AGG - chr4.3:83417385-83417404 MS.gene33475:CDS 30.0%
AGTATTATCAGGTTCATCAT+TGG + chr4.3:83415929-83415948 None:intergenic 30.0%
ATTGGACTATAATGTTAGTC+AGG - chr4.3:83416415-83416434 MS.gene33475:intron 30.0%
GAAAGATGTAACAACAAATG+AGG - chr4.3:83417365-83417384 MS.gene33475:CDS 30.0%
GTAACAACAAATGAGGAAAA+TGG - chr4.3:83417372-83417391 MS.gene33475:CDS 30.0%
TCATTATGTTAATCCCTAGT+AGG - chr4.3:83416339-83416358 MS.gene33475:intron 30.0%
TTTAAGAACTTAGGACAGAA+AGG - chr4.3:83417297-83417316 MS.gene33475:CDS 30.0%
! GCTTTTGCTTTATCAACATT+AGG + chr4.3:83417324-83417343 None:intergenic 30.0%
! GGACTATTCTTTAAGAACTT+AGG - chr4.3:83417288-83417307 MS.gene33475:CDS 30.0%
! TTGAGAATTAGTCTCATAGT+CGG - chr4.3:83416549-83416568 MS.gene33475:intron 30.0%
!! CATCATTAAGTAGTACTGAT+TGG - chr4.3:83417472-83417491 MS.gene33475:CDS 30.0%
!!! TTTGTTTTGTCAAGATGCTA+GGG + chr4.3:83416670-83416689 None:intergenic 30.0%
AACTGTAGTTTGAATCCTAG+GGG - chr4.3:83416472-83416491 MS.gene33475:intron 35.0%
AATGGAGATTGAAGGACAAA+TGG - chr4.3:83417248-83417267 MS.gene33475:CDS 35.0%
ATAGAAGAAGGATAGAAGGA+AGG + chr4.3:83415826-83415845 None:intergenic 35.0%
ATAGAAGGAAGGGTGAAATA+TGG + chr4.3:83415815-83415834 None:intergenic 35.0%
ATGGAAGTTGACAGAAATGA+AGG - chr4.3:83417267-83417286 MS.gene33475:CDS 35.0%
ATTGGTGAAGATGCTCTTAA+AGG - chr4.3:83416099-83416118 MS.gene33475:CDS 35.0%
GAAACTGTAGTTTGAATCCT+AGG - chr4.3:83416470-83416489 MS.gene33475:intron 35.0%
GACAGAAAAAGTTCGTCTTA+CGG - chr4.3:83416008-83416027 MS.gene33475:CDS 35.0%
GGAAAGTGAAAGCAAAGTAA+TGG - chr4.3:83417230-83417249 MS.gene33475:intron 35.0%
TAGAAGAAGGATAGAAGGAA+GGG + chr4.3:83415825-83415844 None:intergenic 35.0%
TCTGAATACTTGACACAGTT+TGG - chr4.3:83416048-83416067 MS.gene33475:CDS 35.0%
TGAAGATGCTCTTAAAGGAT+TGG - chr4.3:83416104-83416123 MS.gene33475:CDS 35.0%
! AGCAAAGTAATGGAGATTGA+AGG - chr4.3:83417240-83417259 MS.gene33475:intron 35.0%
! AGTTCGTCTTACGGATTATT+TGG - chr4.3:83416017-83416036 MS.gene33475:CDS 35.0%
! GTTGATAGAAGAAGGATAGA+AGG + chr4.3:83415830-83415849 None:intergenic 35.0%
! TCTCCTCAAAGTCTCAATTT+TGG + chr4.3:83416773-83416792 None:intergenic 35.0%
!! CTCAAAGTCTCAATTTTGGT+GGG + chr4.3:83416769-83416788 None:intergenic 35.0%
!! GCATAAGTACTGCTTTTAAC+AGG + chr4.3:83417080-83417099 None:intergenic 35.0%
!!! CTTTGTTTTGTCAAGATGCT+AGG + chr4.3:83416671-83416690 None:intergenic 35.0%
AACTTCTTCTTGTGTTGTGG+TGG + chr4.3:83415879-83415898 None:intergenic 40.0%
ACTAGACAGAGTAGAGTTTG+AGG - chr4.3:83417209-83417228 MS.gene33475:intron 40.0%
ACTTCTTCTTGTGTTGTGGT+GGG + chr4.3:83415878-83415897 None:intergenic 40.0%
AGCTAGCTAGAATCTATGCA+TGG + chr4.3:83416193-83416212 None:intergenic 40.0%
AGTTCTTGGAGCTATTCTTG+TGG - chr4.3:83417506-83417525 MS.gene33475:CDS 40.0%
AGTTTCTCAGATACTCCTAC+GGG + chr4.3:83416456-83416475 None:intergenic 40.0%
ATCAGGTTCATCATTGGAGT+TGG + chr4.3:83415923-83415942 None:intergenic 40.0%
CAGAACTTCTTCTTGTGTTG+TGG + chr4.3:83415882-83415901 None:intergenic 40.0%
CAGTTTCTCAGATACTCCTA+CGG + chr4.3:83416457-83416476 None:intergenic 40.0%
CCACCAAAATTGAGACTTTG+AGG - chr4.3:83416767-83416786 MS.gene33475:intron 40.0%
CCTGATAATACTTCACAACC+TGG - chr4.3:83415937-83415956 MS.gene33475:CDS 40.0%
GATAATACTTCACAACCTGG+AGG - chr4.3:83415940-83415959 MS.gene33475:CDS 40.0%
GATGCTAGGGACTAATACAA+AGG + chr4.3:83416657-83416676 None:intergenic 40.0%
GGACTATAATGTTAGTCAGG+TGG - chr4.3:83416418-83416437 MS.gene33475:intron 40.0%
TGGAAGAAAGTTGCAGTTCT+TGG - chr4.3:83417492-83417511 MS.gene33475:CDS 40.0%
! CCAGGTTGTGAAGTATTATC+AGG + chr4.3:83415940-83415959 None:intergenic 40.0%
! CCTCAAAGTCTCAATTTTGG+TGG + chr4.3:83416770-83416789 None:intergenic 40.0%
CTTCTTCTTGTGTTGTGGTG+GGG + chr4.3:83415877-83415896 None:intergenic 45.0%
CTTTCGTTCTCAACCTCCAT+GGG + chr4.3:83416503-83416522 None:intergenic 45.0%
GAGTGAGATGCTAGCATTTG+AGG - chr4.3:83417176-83417195 MS.gene33475:intron 45.0%
GGTCCAAATGACTGAAGTAG+CGG - chr4.3:83416570-83416589 MS.gene33475:intron 45.0%
GTCCAAATGACTGAAGTAGC+GGG - chr4.3:83416571-83416590 MS.gene33475:intron 45.0%
GTGCAGAGTAGTAAAAGTGG+TGG + chr4.3:83415854-83415873 None:intergenic 45.0%
GTTTCTCAGATACTCCTACG+GGG + chr4.3:83416455-83416474 None:intergenic 45.0%
TCCAAATGACTGAAGTAGCG+GGG - chr4.3:83416572-83416591 MS.gene33475:intron 45.0%
TTCTTCTTGTGTTGTGGTGG+GGG + chr4.3:83415876-83415895 None:intergenic 45.0%
! CAAGTTGAGCTAGTAGCTCT+TGG + chr4.3:83415978-83415997 None:intergenic 45.0%
!! GTGGTGGTGTTGATAGAAGA+AGG + chr4.3:83415838-83415857 None:intergenic 45.0%
AATCCTAGGGGAGTAACCCA+TGG - chr4.3:83416484-83416503 MS.gene33475:intron 50.0%
CCATGGAGGTTGAGAACGAA+AGG - chr4.3:83416501-83416520 MS.gene33475:intron 50.0%
CCTTTCGTTCTCAACCTCCA+TGG + chr4.3:83416504-83416523 None:intergenic 50.0%
CTCACCGTGATTAGAGCCTT+TGG + chr4.3:83416623-83416642 None:intergenic 50.0%
GGGGTGCAGAGTAGTAAAAG+TGG + chr4.3:83415857-83415876 None:intergenic 50.0%
TCCCCGCTACTTCAGTCATT+TGG + chr4.3:83416576-83416595 None:intergenic 50.0%
TGGAAGCAATTTGACCCCGT+AGG - chr4.3:83416438-83416457 MS.gene33475:intron 50.0%
! GGTGCCAAAGGCTCTAATCA+CGG - chr4.3:83416616-83416635 MS.gene33475:intron 50.0%
! TGGACTCATTGTGGTGCCAA+AGG - chr4.3:83416604-83416623 MS.gene33475:intron 50.0%
!! ATGCCATGGTGGACTCATTG+TGG - chr4.3:83416595-83416614 MS.gene33475:intron 50.0%
!! TGGCTTTGAACATCACCTCC+AGG + chr4.3:83415958-83415977 None:intergenic 50.0%
GCACCACAATGAGTCCACCA+TGG + chr4.3:83416601-83416620 None:intergenic 55.0%
CCTAGGGGAGTAACCCATGG+AGG - chr4.3:83416487-83416506 MS.gene33475:intron 60.0%
! AAGTAGCGGGGATGCCATGG+TGG - chr4.3:83416584-83416603 MS.gene33475:intron 60.0%
! CCTCCATGGGTTACTCCCCT+AGG + chr4.3:83416490-83416509 None:intergenic 60.0%
! CTGAAGTAGCGGGGATGCCA+TGG - chr4.3:83416581-83416600 MS.gene33475:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 83415796 83417593 83415796 ID=MS.gene33475
chr4.3 mRNA 83415796 83417593 83415796 ID=MS.gene33475.t1;Parent=MS.gene33475
chr4.3 exon 83417246 83417593 83417246 ID=MS.gene33475.t1.exon1;Parent=MS.gene33475.t1
chr4.3 CDS 83417246 83417593 83417246 ID=cds.MS.gene33475.t1;Parent=MS.gene33475.t1
chr4.3 exon 83415796 83416230 83415796 ID=MS.gene33475.t1.exon2;Parent=MS.gene33475.t1
chr4.3 CDS 83415796 83416230 83415796 ID=cds.MS.gene33475.t1;Parent=MS.gene33475.t1
Gene Sequence

>MS.gene33475

ATGATTGCTCTCAAAGCCATATTTCACCCTTCCTTCTATCCTTCTTCTATCAACACCACCACTTTTACTACTCTGCACCCCCACCACAACACAAGAAGAAGTTCTGTTTTTCTCTGTCTTTGTACCAACTCCAATGATGAACCTGATAATACTTCACAACCTGGAGGTGATGTTCAAAGCCAAGAGCTACTAGCTCAACTTGCAATGCTTGAGACAGAAAAAGTTCGTCTTACGGATTATTTGGATGAAAGATCTGAATACTTGACACAGTTTGGTGAAGAAGCTAAAGCTGAAATTGATAAGATTGGTGAAGATGCTCTTAAAGGATTGGATGAAGCTAGTGATAGAGTATGTATGCACTTTTCACTCACTATTTACTTGTTCAATTTGTTAACCATGCATAGATTCTAGCTAGCTACAGAGTTATTCATTTTAGTACTTTACATTCCAGTAAACCATAATTCAGTTTTTAAAGTATATGAACATAACAAATAATTGTTAAAGTATGTACAATTTATCTTGTTCTTTAAAGTATATGAAAATTCATTATGTTAATCCCTAGTAGGAACTATACTTTAAAATTATTTATTTTATATACTTTAGATAAAATGAGAGAGAGATTGGACTATAATGTTAGTCAGGTGGAAGCAATTTGACCCCGTAGGAGTATCTGAGAAACTGTAGTTTGAATCCTAGGGGAGTAACCCATGGAGGTTGAGAACGAAAGGATAAAATATTATTTAATCAGTCGAATTGAGAATTAGTCTCATAGTCGGTCCAAATGACTGAAGTAGCGGGGATGCCATGGTGGACTCATTGTGGTGCCAAAGGCTCTAATCACGGTGAGCATATGAAAATCCTTTGTATTAGTCCCTAGCATCTTGACAAAACAAAGTCTATCTAAAGTGACGAATTTTATTTAATTTTGAGATAATCGTTTTTTTTTATTTTTTTGAAGAAGCTAAACTAACCCACCAAAATTGAGACTTTGAGGAGAAGCATACTCATTATTTTTAAATACTATAGAGACTAAAGTTGAAGATAGTGATACTTTAGAAACTAAAATTGAAGATAGCGATACTTTAGAGACTAAATTGATAATGTATTCTCTTACAATCCATATTTTTTTATAAGATATGTTAATGTACTTTTAATTCTATTTTAATTGTTTAATTATTGATTTTGTCAGAAAAATTGTTTAATTATTGATTGATTTCTTAGATTATATGCTTACTTTGTACACTTATCATATGGAGTATAGAATATACAAACGCAAATATGCCTGTTAAAAGCAGTACTTATGCAAAATTTGCTTGAAAATTACTTCAATAATGACCCTTTTACATTCATTTGTGATTAATAGATAACAGCAAAAATAGAGAGTGAGATGCTAGCATTTGAGGAATCAAATGAACTAGACAGAGTAGAGTTTGAGGAAAGTGAAAGCAAAGTAATGGAGATTGAAGGACAAATGGAAGTTGACAGAAATGAAGGACTATTCTTTAAGAACTTAGGACAGAAAGGACCTAATGTTGATAAAGCAAAAGCCAAAGAAGAAATAGAAAAGATGAAAGATGTAACAACAAATGAGGAAAATGGAAGAAAAACAAGGAAAAATGTTTACTTATTCTTCATTGGTCTTTTTACATATGGAATAGTTGGTTCTATTAATGTCTCATCATTAAGTAGTACTGATTGGAAGAAAGTTGCAGTTCTTGGAGCTATTCTTGTGGCTTTGTTTTCTCAGTTCATTTATGAACAAAATAAAGACAATAATCAGAAAGATGATGAACAATGA

Protein sequence

>MS.gene33475.t1

MIALKAIFHPSFYPSSINTTTFTTLHPHHNTRRSSVFLCLCTNSNDEPDNTSQPGGDVQSQELLAQLAMLETEKVRLTDYLDERSEYLTQFGEEAKAEIDKIGEDALKGLDEASDRITAKIESEMLAFEESNELDRVEFEESESKVMEIEGQMEVDRNEGLFFKNLGQKGPNVDKAKAKEEIEKMKDVTTNEENGRKTRKNVYLFFIGLFTYGIVGSINVSSLSSTDWKKVAVLGAILVALFSQFIYEQNKDNNQKDDEQ