AlfalfaGEDB | MS.gene33853

M. sativa cultivar XinJiangDaYe / MS.gene33853

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF01535.23 PF12854.10 PF13041.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33853.t1	XP_003608278.1	98.2	341	5	1	72	412	495	834	5.20E-74	288.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33853.t1	Q0WPZ6	63.0	46	17	0	261	306	682	727	1.2e-07	59.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33853.t1	Q2HTU1	98.2	341	5	1	72	412	495	834	3.8e-74	288.5

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene33853	0.801142	1.04E-48	-1.69E-46
MS.gene049254	MS.gene33853	0.868038	8.79E-66	-1.69E-46
MS.gene049271	MS.gene33853	0.803988	2.68E-49	-1.69E-46
MS.gene049272	MS.gene33853	0.80119	1.01E-48	-1.69E-46
MS.gene049404	MS.gene33853	0.801813	7.54E-49	-1.69E-46
MS.gene049435	MS.gene33853	0.814568	1.44E-51	-1.69E-46
MS.gene049452	MS.gene33853	0.80014	1.66E-48	-1.69E-46
MS.gene049501	MS.gene33853	0.803264	3.79E-49	-1.69E-46
MS.gene049567	MS.gene33853	0.809881	1.52E-50	-1.69E-46
MS.gene049666	MS.gene33853	0.804818	1.80E-49	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33853.t1	MTR_4g091600	99.029	412	3	1	1	412	424	834	0.0	838
MS.gene33853.t1	MTR_4g091600	27.062	388	233	4	1	373	142	494	5.36e-41	156
MS.gene33853.t1	MTR_4g091600	24.638	414	269	8	1	373	245	656	4.73e-30	124
MS.gene33853.t1	MTR_1g033960	30.691	391	239	5	1	387	220	582	2.97e-53	190
MS.gene33853.t1	MTR_1g033960	30.104	289	187	5	95	378	186	464	4.27e-35	138
MS.gene33853.t1	MTR_1g033960	24.542	273	199	3	100	366	152	423	2.37e-29	121
MS.gene33853.t1	MTR_1g033960	28.030	396	178	7	1	346	395	733	2.18e-27	115
MS.gene33853.t1	MTR_1g033960	24.900	249	172	4	136	374	118	361	6.41e-16	80.5
MS.gene33853.t1	MTR_0014s0180	30.848	389	252	5	1	374	122	508	1.51e-47	171
MS.gene33853.t1	MTR_0014s0180	29.000	400	227	4	5	369	91	468	7.65e-43	158

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33853.t1	AT2G17140	56.490	416	177	4	1	414	422	835	2.36e-158	469
MS.gene33853.t1	AT2G17140	25.594	379	221	4	1	375	173	494	2.53e-34	136
MS.gene33853.t1	AT2G17140	26.196	397	244	6	5	374	212	586	1.79e-31	128
MS.gene33853.t1	AT2G17140	27.857	280	196	4	99	373	144	422	3.63e-27	115
MS.gene33853.t1	AT2G17140	24.638	345	246	5	35	374	53	388	1.29e-22	101
MS.gene33853.t1	AT2G17140	56.490	416	177	4	1	414	422	835	2.36e-158	469
MS.gene33853.t1	AT2G17140	25.594	379	221	4	1	375	173	494	2.53e-34	136
MS.gene33853.t1	AT2G17140	26.196	397	244	6	5	374	212	586	1.79e-31	128
MS.gene33853.t1	AT2G17140	27.857	280	196	4	99	373	144	422	3.63e-27	115
MS.gene33853.t1	AT2G17140	24.638	345	246	5	35	374	53	388	1.29e-22	101

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Find 92 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAACAGAACCCAAAAG+TGG	0.526556	4.3:+22448076	None:intergenic
AAAGAAATTTATTGAGATGA+TGG	0.408816	4.3:-22448982	MS.gene33853:CDS
AACTGTACAAGGCTTCTTTG+TGG	0.506728	4.3:+22448526	None:intergenic
AACTTATAATTCATTGATCT+TGG	0.265731	4.3:-22448829	MS.gene33853:CDS
AATTAACAGGTATGACCATA+TGG	0.475134	4.3:-22448121	MS.gene33853:CDS
AATTAACTAGGCCAGCAACC+GGG	0.632063	4.3:+22449099	None:intergenic
AATTCATTGATCTTGGGGTT+AGG	0.445013	4.3:-22448822	MS.gene33853:CDS
ACAAACTTTGCAAGGATGGA+AGG	0.526029	4.3:-22448389	MS.gene33853:CDS
ACAAGGATGGTGGAAGTGAT+TGG	0.479211	4.3:-22448152	MS.gene33853:CDS
ACCATTGACCACGATATTAC+AGG	0.397570	4.3:+22449208	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTAACAGGTATGACCATA+TGG	-	chr4.3:22449279-22449298	MS.gene33853:CDS	30.0%
ATTGGCAGGATATAATTAAC+AGG	-	chr4.3:22449266-22449285	MS.gene33853:CDS	30.0%
CATTTCATGTAAAAGAGAAG+TGG	+	chr4.3:22448732-22448751	None:intergenic	30.0%
CTTGCAAAGTCCATTAATTA+AGG	+	chr4.3:22448375-22448394	None:intergenic	30.0%
GAAAGATAGAGTTTCCATTT+CGG	+	chr4.3:22449216-22449235	None:intergenic	30.0%
GTATTGAATTTCTGCAAACA+AGG	-	chr4.3:22448482-22448501	MS.gene33853:CDS	30.0%
TTTACGTGTATTGAAAGACA+TGG	-	chr4.3:22448520-22448539	MS.gene33853:CDS	30.0%
TTTGATTGACAAACTTTGCA+AGG	-	chr4.3:22449003-22449022	MS.gene33853:CDS	30.0%
AAAAGAACAGAACCCAAAAG+TGG	+	chr4.3:22449327-22449346	None:intergenic	35.0%
AATTCATTGATCTTGGGGTT+AGG	-	chr4.3:22448578-22448597	MS.gene33853:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	22448053	22449369	22448053	ID=MS.gene33853
chr4.3	mRNA	22448053	22449369	22448053	ID=MS.gene33853.t1;Parent=MS.gene33853
chr4.3	exon	22448053	22449369	22448053	ID=MS.gene33853.t1.exon1;Parent=MS.gene33853.t1
chr4.3	CDS	22448053	22449369	22448053	ID=cds.MS.gene33853.t1;Parent=MS.gene33853.t1

Gene Sequence

>MS.gene33853

ATGATTAGGAAAGGTTGTCATCCAAATACTTATACTTGCAATACGTTGCTGAATAGCCTGTGGAAAGAGGGGAGGAAGTCAGAGGCTGAGGAAATGCTGCAAAAGATGAACGAAAAGAGTTATCAGTTAGATACCGTGACCTGTAATATCGTGGTCAATGGTCTGTGTAGAAATGGAGAATTGGAGAAAGCAAGTGAAGTTGTAAGTGAGATGTGGACTGATGGAACAAATTCCCTTGGTAAAGAAAACCCGGTTGCTGGCCTAGTTAATTCAATCCATAATGTATCGACCAATGTACCTGATGTGATCACTTATACAACCTTAATTAATGGACTTTGCAAGGTTGGGAAACTAGAGGAAGCCAAAAAGAAATTTATTGAGATGATGGCGAAAAACTTACACCCTGATTCTGTGACCTATGATACGTTTGTATTGAATTTCTGCAAACAAGGGAAGATATCATCGGCTTTACGTGTATTGAAAGACATGGAGAGAAATGGTTGCAGCAAGACTCTTCAAACTTATAATTCATTGATCTTGGGGTTAGGAAGTAAAGGGCAAATATTTGAAATGTACGGATTGATGGATGAGATGAGAGAAAGAGGAATACATCCAGATATTTGCACTTACAATAATATGATCAATTGTCTTTGCGAAGGAGGAAAAACAAAGGATGCCACTTCTCTTTTACATGAAATGTTGGATAAGGGAGTCGTCTCTCCCAATGTATCATCCTTCAAAATATTGATTAAAGCATTCTGCAAGTCTGAAGATTTTAAAGTAGCATGTGAACTTTTTGATGTAGCTCTGAGTGTATGTGGCCACAAAGAAGCCTTGTACAGTTTGATGTTCAACGAATTACTTGCTGGGGGAAAACTCTCTGATGCAAAAGAGCTATTTGAAGCTTCGTTAGAAAGATCTCTCTTATCAAAGAACTTTATGTATGAAGATTTGATTGACAAACTTTGCAAGGATGGAAGGTTAGACGATGCTCACGGCCTTCTTCAGAAATTGATTGATAAGGGATATTGCTTCGACCATTCATCATTCATACCTGTGATTGATGGCTTAAGTAAAAGGGGAAACAAACAGCAAGCTGATGAACTAGCAAGGATAATGGAATTGGCTTTAGAAGAAGATAAAACTTTTGATAGGACGTACCGAAATGGAAACTCTATCTTTCGTCGAAAATTACACAAGGATGGTGGAAGTGATTGGCAGGATATAATTAACAGGTATGACCATATGGTAGTGAAGATAGTCTTTTCATTCCACTTTTGGGTTCTGTTCTTTTATAAAAGAGATGTTTTTAACTAG

Protein sequence

>MS.gene33853.t1

MIRKGCHPNTYTCNTLLNSLWKEGRKSEAEEMLQKMNEKSYQLDTVTCNIVVNGLCRNGELEKASEVVSEMWTDGTNSLGKENPVAGLVNSIHNVSTNVPDVITYTTLINGLCKVGKLEEAKKKFIEMMAKNLHPDSVTYDTFVLNFCKQGKISSALRVLKDMERNGCSKTLQTYNSLILGLGSKGQIFEMYGLMDEMRERGIHPDICTYNNMINCLCEGGKTKDATSLLHEMLDKGVVSPNVSSFKILIKAFCKSEDFKVACELFDVALSVCGHKEALYSLMFNELLAGGKLSDAKELFEASLERSLLSKNFMYEDLIDKLCKDGRLDDAHGLLQKLIDKGYCFDHSSFIPVIDGLSKRGNKQQADELARIMELALEEDKTFDRTYRNGNSIFRRKLHKDGGSDWQDIINRYDHMVVKIVFSFHFWVLFFYKRDVFN