Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36339.t1 | XP_003597705.1 | 96.5 | 347 | 12 | 0 | 1 | 347 | 1 | 347 | 1.20E-195 | 692.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36339.t1 | Q9LTW0 | 64.5 | 324 | 113 | 2 | 21 | 344 | 27 | 348 | 1.9e-120 | 433.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36339.t1 | G7IUN2 | 96.5 | 347 | 12 | 0 | 1 | 347 | 1 | 347 | 8.9e-196 | 692.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene36339 | 0.800744 | 1.25E-48 | -1.69E-46 |
| MS.gene049217 | MS.gene36339 | 0.812808 | 3.52E-51 | -1.69E-46 |
| MS.gene049222 | MS.gene36339 | 0.837285 | 5.69E-57 | -1.69E-46 |
| MS.gene049232 | MS.gene36339 | 0.808869 | 2.51E-50 | -1.69E-46 |
| MS.gene049285 | MS.gene36339 | 0.817912 | 2.58E-52 | -1.69E-46 |
| MS.gene049320 | MS.gene36339 | 0.811059 | 8.46E-51 | -1.69E-46 |
| MS.gene049356 | MS.gene36339 | 0.819849 | 9.36E-53 | -1.69E-46 |
| MS.gene049369 | MS.gene36339 | 0.823897 | 1.08E-53 | -1.69E-46 |
| MS.gene049396 | MS.gene36339 | 0.850226 | 1.95E-60 | -1.69E-46 |
| MS.gene049427 | MS.gene36339 | 0.804684 | 1.92E-49 | -1.69E-46 |
| MS.gene049609 | MS.gene36339 | 0.884793 | 1.42E-71 | -1.69E-46 |
| MS.gene049633 | MS.gene36339 | 0.818728 | 1.68E-52 | -1.69E-46 |
| MS.gene049805 | MS.gene36339 | 0.869242 | 3.58E-66 | -1.69E-46 |
| MS.gene050323 | MS.gene36339 | 0.802592 | 5.22E-49 | -1.69E-46 |
| MS.gene050593 | MS.gene36339 | 0.868908 | 4.60E-66 | -1.69E-46 |
| MS.gene050662 | MS.gene36339 | 0.81587 | 7.41E-52 | -1.69E-46 |
| MS.gene050670 | MS.gene36339 | 0.813555 | 2.41E-51 | -1.69E-46 |
| MS.gene050686 | MS.gene36339 | 0.81704 | 4.05E-52 | -1.69E-46 |
| MS.gene050690 | MS.gene36339 | 0.806641 | 7.45E-50 | -1.69E-46 |
| MS.gene050747 | MS.gene36339 | 0.848894 | 4.58E-60 | -1.69E-46 |
| MS.gene050772 | MS.gene36339 | 0.881041 | 3.34E-70 | -1.69E-46 |
| MS.gene05080 | MS.gene36339 | 0.82761 | 1.42E-54 | -1.69E-46 |
| MS.gene050950 | MS.gene36339 | 0.841003 | 6.18E-58 | -1.69E-46 |
| MS.gene05105 | MS.gene36339 | 0.856457 | 3.18E-62 | -1.69E-46 |
| MS.gene051167 | MS.gene36339 | -0.807724 | 4.40E-50 | -1.69E-46 |
| MS.gene051387 | MS.gene36339 | 0.839583 | 1.45E-57 | -1.69E-46 |
| MS.gene051666 | MS.gene36339 | 0.822876 | 1.87E-53 | -1.69E-46 |
| MS.gene051692 | MS.gene36339 | 0.817677 | 2.91E-52 | -1.69E-46 |
| MS.gene051838 | MS.gene36339 | 0.834972 | 2.20E-56 | -1.69E-46 |
| MS.gene051858 | MS.gene36339 | 0.826629 | 2.44E-54 | -1.69E-46 |
| MS.gene052346 | MS.gene36339 | 0.800579 | 1.35E-48 | -1.69E-46 |
| MS.gene052379 | MS.gene36339 | 0.808315 | 3.29E-50 | -1.69E-46 |
| MS.gene052421 | MS.gene36339 | 0.83948 | 1.55E-57 | -1.69E-46 |
| MS.gene052484 | MS.gene36339 | 0.818639 | 1.76E-52 | -1.69E-46 |
| MS.gene052501 | MS.gene36339 | 0.814054 | 1.87E-51 | -1.69E-46 |
| MS.gene052503 | MS.gene36339 | 0.8165 | 5.36E-52 | -1.69E-46 |
| MS.gene052505 | MS.gene36339 | 0.819264 | 1.27E-52 | -1.69E-46 |
| MS.gene052506 | MS.gene36339 | 0.82428 | 8.79E-54 | -1.69E-46 |
| MS.gene052615 | MS.gene36339 | 0.85625 | 3.65E-62 | -1.69E-46 |
| MS.gene052822 | MS.gene36339 | 0.827252 | 1.73E-54 | -1.69E-46 |
| MS.gene052823 | MS.gene36339 | 0.808033 | 3.78E-50 | -1.69E-46 |
| MS.gene052825 | MS.gene36339 | 0.800174 | 1.63E-48 | -1.69E-46 |
| MS.gene052916 | MS.gene36339 | 0.83813 | 3.45E-57 | -1.69E-46 |
| MS.gene053018 | MS.gene36339 | 0.805718 | 1.17E-49 | -1.69E-46 |
| MS.gene053103 | MS.gene36339 | 0.803474 | 3.43E-49 | -1.69E-46 |
| MS.gene053104 | MS.gene36339 | 0.830966 | 2.18E-55 | -1.69E-46 |
| MS.gene053207 | MS.gene36339 | 0.83127 | 1.84E-55 | -1.69E-46 |
| MS.gene053213 | MS.gene36339 | 0.846328 | 2.33E-59 | -1.69E-46 |
| MS.gene053286 | MS.gene36339 | 0.835732 | 1.41E-56 | -1.69E-46 |
| MS.gene053436 | MS.gene36339 | 0.815821 | 7.59E-52 | -1.69E-46 |
| MS.gene053449 | MS.gene36339 | 0.854521 | 1.16E-61 | -1.69E-46 |
| MS.gene053574 | MS.gene36339 | 0.876228 | 1.65E-68 | -1.69E-46 |
| MS.gene053626 | MS.gene36339 | 0.823507 | 1.33E-53 | -1.69E-46 |
| MS.gene053632 | MS.gene36339 | 0.83595 | 1.24E-56 | -1.69E-46 |
| MS.gene053824 | MS.gene36339 | 0.821925 | 3.12E-53 | -1.69E-46 |
| MS.gene053912 | MS.gene36339 | 0.845728 | 3.39E-59 | -1.69E-46 |
| MS.gene053916 | MS.gene36339 | 0.823349 | 1.45E-53 | -1.69E-46 |
| MS.gene054003 | MS.gene36339 | 0.811209 | 7.85E-51 | -1.69E-46 |
| MS.gene054595 | MS.gene36339 | 0.81835 | 2.05E-52 | -1.69E-46 |
| MS.gene054600 | MS.gene36339 | 0.809151 | 2.18E-50 | -1.69E-46 |
| MS.gene054642 | MS.gene36339 | 0.826072 | 3.31E-54 | -1.69E-46 |
| MS.gene054927 | MS.gene36339 | 0.804138 | 2.49E-49 | -1.69E-46 |
| MS.gene05494 | MS.gene36339 | 0.800595 | 1.34E-48 | -1.69E-46 |
| MS.gene05505 | MS.gene36339 | 0.872212 | 3.78E-67 | -1.69E-46 |
| MS.gene055206 | MS.gene36339 | 0.835746 | 1.40E-56 | -1.69E-46 |
| MS.gene055224 | MS.gene36339 | 0.808146 | 3.57E-50 | -1.69E-46 |
| MS.gene055289 | MS.gene36339 | 0.833516 | 5.10E-56 | -1.69E-46 |
| MS.gene055486 | MS.gene36339 | 0.836762 | 7.74E-57 | -1.69E-46 |
| MS.gene055502 | MS.gene36339 | 0.826043 | 3.37E-54 | -1.69E-46 |
| MS.gene055577 | MS.gene36339 | 0.812108 | 5.00E-51 | -1.69E-46 |
| MS.gene055647 | MS.gene36339 | 0.811474 | 6.88E-51 | -1.69E-46 |
| MS.gene055722 | MS.gene36339 | 0.872703 | 2.59E-67 | -1.69E-46 |
| MS.gene055909 | MS.gene36339 | 0.803886 | 2.81E-49 | -1.69E-46 |
| MS.gene055911 | MS.gene36339 | 0.800152 | 1.65E-48 | -1.69E-46 |
| MS.gene05592 | MS.gene36339 | 0.840356 | 9.13E-58 | -1.69E-46 |
| MS.gene055941 | MS.gene36339 | 0.851908 | 6.53E-61 | -1.69E-46 |
| MS.gene056166 | MS.gene36339 | 0.817928 | 2.56E-52 | -1.69E-46 |
| MS.gene056374 | MS.gene36339 | 0.803411 | 3.53E-49 | -1.69E-46 |
| MS.gene056422 | MS.gene36339 | 0.825058 | 5.76E-54 | -1.69E-46 |
| MS.gene056448 | MS.gene36339 | 0.87773 | 4.97E-69 | -1.69E-46 |
| MS.gene056487 | MS.gene36339 | 0.834094 | 3.66E-56 | -1.69E-46 |
| MS.gene056511 | MS.gene36339 | 0.896892 | 2.38E-76 | -1.69E-46 |
| MS.gene05664 | MS.gene36339 | 0.814858 | 1.24E-51 | -1.69E-46 |
| MS.gene056657 | MS.gene36339 | 0.820154 | 7.97E-53 | -1.69E-46 |
| MS.gene05666 | MS.gene36339 | 0.81848 | 1.92E-52 | -1.69E-46 |
| MS.gene057076 | MS.gene36339 | 0.883609 | 3.88E-71 | -1.69E-46 |
| MS.gene057086 | MS.gene36339 | 0.815336 | 9.74E-52 | -1.69E-46 |
| MS.gene057146 | MS.gene36339 | 0.822069 | 2.89E-53 | -1.69E-46 |
| MS.gene057331 | MS.gene36339 | 0.846481 | 2.11E-59 | -1.69E-46 |
| MS.gene05735 | MS.gene36339 | 0.815272 | 1.01E-51 | -1.69E-46 |
| MS.gene057416 | MS.gene36339 | 0.870952 | 9.89E-67 | -1.69E-46 |
| MS.gene057734 | MS.gene36339 | 0.85558 | 5.73E-62 | -1.69E-46 |
| MS.gene057856 | MS.gene36339 | 0.830103 | 3.55E-55 | -1.69E-46 |
| MS.gene057926 | MS.gene36339 | 0.810287 | 1.24E-50 | -1.69E-46 |
| MS.gene058019 | MS.gene36339 | 0.819007 | 1.46E-52 | -1.69E-46 |
| MS.gene058130 | MS.gene36339 | 0.805894 | 1.07E-49 | -1.69E-46 |
| MS.gene058338 | MS.gene36339 | 0.80882 | 2.57E-50 | -1.69E-46 |
| MS.gene05837 | MS.gene36339 | 0.800965 | 1.12E-48 | -1.69E-46 |
| MS.gene058419 | MS.gene36339 | 0.828879 | 7.03E-55 | -1.69E-46 |
| MS.gene058838 | MS.gene36339 | 0.816057 | 6.73E-52 | -1.69E-46 |
| MS.gene05883 | MS.gene36339 | 0.806665 | 7.37E-50 | -1.69E-46 |
| MS.gene059023 | MS.gene36339 | 0.802678 | 5.01E-49 | -1.69E-46 |
| MS.gene059029 | MS.gene36339 | 0.802508 | 5.43E-49 | -1.69E-46 |
| MS.gene05913 | MS.gene36339 | 0.808608 | 2.85E-50 | -1.69E-46 |
| MS.gene05934 | MS.gene36339 | 0.845268 | 4.51E-59 | -1.69E-46 |
| MS.gene059366 | MS.gene36339 | 0.819673 | 1.03E-52 | -1.69E-46 |
| MS.gene059367 | MS.gene36339 | 0.816072 | 6.68E-52 | -1.69E-46 |
| MS.gene059368 | MS.gene36339 | 0.824368 | 8.38E-54 | -1.69E-46 |
| MS.gene059455 | MS.gene36339 | 0.851627 | 7.85E-61 | -1.69E-46 |
| MS.gene059536 | MS.gene36339 | 0.821981 | 3.02E-53 | -1.69E-46 |
| MS.gene059674 | MS.gene36339 | 0.800774 | 1.23E-48 | -1.69E-46 |
| MS.gene059688 | MS.gene36339 | 0.820409 | 6.96E-53 | -1.69E-46 |
| MS.gene059711 | MS.gene36339 | 0.84577 | 3.30E-59 | -1.69E-46 |
| MS.gene059718 | MS.gene36339 | 0.839487 | 1.54E-57 | -1.69E-46 |
| MS.gene059902 | MS.gene36339 | 0.819617 | 1.06E-52 | -1.69E-46 |
| MS.gene060482 | MS.gene36339 | 0.84862 | 5.46E-60 | -1.69E-46 |
| MS.gene060804 | MS.gene36339 | 0.867617 | 1.20E-65 | -1.69E-46 |
| MS.gene060818 | MS.gene36339 | 0.846194 | 2.53E-59 | -1.69E-46 |
| MS.gene060835 | MS.gene36339 | 0.87317 | 1.81E-67 | -1.69E-46 |
| MS.gene060837 | MS.gene36339 | 0.861832 | 7.78E-64 | -1.69E-46 |
| MS.gene06092 | MS.gene36339 | 0.817408 | 3.35E-52 | -1.69E-46 |
| MS.gene061041 | MS.gene36339 | 0.843631 | 1.24E-58 | -1.69E-46 |
| MS.gene061134 | MS.gene36339 | 0.811944 | 5.43E-51 | -1.69E-46 |
| MS.gene061200 | MS.gene36339 | 0.809457 | 1.87E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36339.t1 | MTR_2g101400 | 96.542 | 347 | 12 | 0 | 1 | 347 | 1 | 347 | 0.0 | 700 |
| MS.gene36339.t1 | MTR_7g086110 | 67.081 | 322 | 105 | 1 | 26 | 346 | 4 | 325 | 1.02e-164 | 462 |
| MS.gene36339.t1 | MTR_2g067760 | 62.500 | 328 | 121 | 2 | 17 | 344 | 21 | 346 | 2.49e-156 | 442 |
| MS.gene36339.t1 | MTR_2g451450 | 58.086 | 303 | 126 | 1 | 27 | 329 | 30 | 331 | 2.01e-127 | 368 |
| MS.gene36339.t1 | MTR_2g451450 | 62.882 | 229 | 84 | 1 | 113 | 341 | 1 | 228 | 1.50e-104 | 306 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36339.t1 | AT3G12740 | 64.024 | 328 | 116 | 2 | 17 | 344 | 23 | 348 | 1.29e-156 | 443 |
| MS.gene36339.t1 | AT1G54320 | 62.805 | 328 | 120 | 2 | 17 | 344 | 22 | 347 | 4.71e-154 | 437 |
| MS.gene36339.t1 | AT1G79450 | 62.538 | 331 | 122 | 2 | 17 | 347 | 22 | 350 | 1.27e-152 | 433 |
| MS.gene36339.t1 | AT1G16360 | 62.006 | 329 | 123 | 2 | 16 | 344 | 21 | 347 | 5.61e-152 | 431 |
| MS.gene36339.t1 | AT1G16360 | 61.755 | 319 | 120 | 2 | 26 | 344 | 17 | 333 | 2.99e-146 | 416 |
| MS.gene36339.t1 | AT1G16360 | 60.855 | 304 | 117 | 2 | 26 | 329 | 17 | 318 | 5.90e-136 | 390 |
| MS.gene36339.t1 | AT1G79450 | 61.649 | 279 | 105 | 2 | 69 | 347 | 7 | 283 | 2.99e-124 | 358 |
| MS.gene36339.t1 | AT5G46150 | 52.632 | 342 | 157 | 3 | 1 | 341 | 1 | 338 | 2.01e-119 | 348 |
| MS.gene36339.t1 | AT5G46150 | 52.632 | 342 | 157 | 3 | 1 | 341 | 1 | 338 | 2.01e-119 | 348 |
| MS.gene36339.t1 | AT5G46150 | 52.727 | 330 | 154 | 2 | 1 | 329 | 1 | 329 | 2.80e-116 | 340 |
| MS.gene36339.t1 | AT1G16360 | 55.502 | 209 | 91 | 2 | 26 | 234 | 17 | 223 | 1.11e-79 | 243 |
| MS.gene36339.t1 | AT1G16360 | 55.502 | 209 | 91 | 2 | 26 | 234 | 17 | 223 | 1.11e-79 | 243 |
| MS.gene36339.t1 | AT5G46150 | 49.145 | 234 | 117 | 2 | 1 | 233 | 1 | 233 | 3.24e-74 | 229 |
Find 59 sgRNAs with CRISPR-Local
Find 130 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGACATACATTTAGGTTTCT+TGG | 0.260587 | 2.2:+2155625 | None:intergenic |
| GGAATCATCTTTATCCCTAT+TGG | 0.278510 | 2.2:-2155028 | MS.gene36339:CDS |
| CTATCCTTGTTCTGTGCTAT+AGG | 0.299641 | 2.2:-2153455 | MS.gene36339:CDS |
| ATTGCCACATTTACTGCAAT+TGG | 0.348370 | 2.2:-2155049 | MS.gene36339:CDS |
| TTTCAGAGTGGAGGTTTGAT+TGG | 0.358409 | 2.2:-2154035 | MS.gene36339:CDS |
| ACCCATCCAGGTGTTAGAAT+TGG | 0.365146 | 2.2:+2155301 | None:intergenic |
| GTTGTCCATTGAAAATTTGT+AGG | 0.382564 | 2.2:+2154142 | None:intergenic |
| AACAATGATGCAAGACCAAT+AGG | 0.394455 | 2.2:+2155013 | None:intergenic |
| AACAAGAGGATCTGATTGTT+TGG | 0.396536 | 2.2:-2153685 | MS.gene36339:CDS |
| GGGATTGCATACCTTGTTGT+TGG | 0.410908 | 2.2:-2153482 | MS.gene36339:CDS |
| CAGAGTGGAGGTTTGATTGG+AGG | 0.413776 | 2.2:-2154032 | MS.gene36339:CDS |
| CCAACTTTCAGAAAACTGTA+TGG | 0.436379 | 2.2:-2153644 | MS.gene36339:CDS |
| ACAAGACTTGCACCCACAAA+TGG | 0.438149 | 2.2:-2154795 | MS.gene36339:CDS |
| CAACTACAACTTGGATTGGT+GGG | 0.445444 | 2.2:-2153520 | MS.gene36339:CDS |
| ATGAGGCCACAAGGAACAAT+TGG | 0.445844 | 2.2:+2154188 | None:intergenic |
| TTCCTTGTGGCCTCATTGCA+TGG | 0.449722 | 2.2:-2154181 | MS.gene36339:CDS |
| TACAACACATACGAGTTTCA+AGG | 0.464659 | 2.2:-2153563 | MS.gene36339:CDS |
| GTTAGAATTGGTTTCCATGC+AGG | 0.464924 | 2.2:+2155313 | None:intergenic |
| GGGATCCATCCTACTTATCA+TGG | 0.480170 | 2.2:-2153112 | MS.gene36339:CDS |
| ATTGCATACCTTGTTGTTGG+TGG | 0.482393 | 2.2:-2153479 | MS.gene36339:CDS |
| GTGGGTGCAAGTCTTGTTAG+TGG | 0.484884 | 2.2:+2154801 | None:intergenic |
| CTATCAACTACAACTTGGAT+TGG | 0.485356 | 2.2:-2153524 | MS.gene36339:CDS |
| TTATCATGGAACAGAAATCC+AGG | 0.490301 | 2.2:-2153098 | MS.gene36339:CDS |
| TGATGAAGTCATATCCATCA+TGG | 0.492388 | 2.2:+2155685 | None:intergenic |
| TTTGATAGTAATTATCAAGC+TGG | 0.503169 | 2.2:+2154644 | None:intergenic |
| CTGTTCCATGATAAGTAGGA+TGG | 0.511626 | 2.2:+2153107 | None:intergenic |
| ATTTCTGTTCCATGATAAGT+AGG | 0.512059 | 2.2:+2153103 | None:intergenic |
| GTTTCAAGGTAGGAAGAAGT+TGG | 0.512965 | 2.2:-2153549 | MS.gene36339:CDS |
| ACAATGATGCAAGACCAATA+GGG | 0.513968 | 2.2:+2155014 | None:intergenic |
| ACCAATTCTAACACCTGGAT+GGG | 0.523126 | 2.2:-2155302 | MS.gene36339:intron |
| TGGTTCTATCAACTACAACT+TGG | 0.538526 | 2.2:-2153529 | MS.gene36339:CDS |
| ATGTACTAACAATCCATTTG+TGG | 0.539027 | 2.2:+2154782 | None:intergenic |
| CAACTTTCAGAAAACTGTAT+GGG | 0.539444 | 2.2:-2153643 | MS.gene36339:CDS |
| GATATGACTTCATCAAAAGA+TGG | 0.544518 | 2.2:-2155677 | MS.gene36339:CDS |
| AACCAATTCTAACACCTGGA+TGG | 0.544797 | 2.2:-2155303 | MS.gene36339:CDS |
| GTTGCATATTATACCCATCC+AGG | 0.548338 | 2.2:+2155289 | None:intergenic |
| TCAACTACAACTTGGATTGG+TGG | 0.549590 | 2.2:-2153521 | MS.gene36339:CDS |
| AACTGCATCTTTAGCATCAG+AGG | 0.555713 | 2.2:+2154843 | None:intergenic |
| AGTAGACAAAACAGAAGGAA+AGG | 0.558583 | 2.2:-2154213 | MS.gene36339:CDS |
| TTGTGGCGTAAGTCAGCTGA+AGG | 0.559346 | 2.2:-2154257 | MS.gene36339:CDS |
| CTCCATGCAATGAGGCCACA+AGG | 0.565861 | 2.2:+2154179 | None:intergenic |
| GCATCAGTTGAGTGAACAAG+AGG | 0.580136 | 2.2:-2153699 | MS.gene36339:intron |
| TGGAAACCAATTCTAACACC+TGG | 0.581766 | 2.2:-2155307 | MS.gene36339:CDS |
| GAATCCTATAGCACAGAACA+AGG | 0.595106 | 2.2:+2153451 | None:intergenic |
| ATTACTATCAAAATCATCGC+CGG | 0.596581 | 2.2:-2154633 | MS.gene36339:intron |
| GATTCCAATTGCAGTAAATG+TGG | 0.597750 | 2.2:+2155045 | None:intergenic |
| TTCTGTATGTGATCAAGCCA+AGG | 0.598227 | 2.2:-2153424 | MS.gene36339:intron |
| CACAGCAAGAACTACCTGCA+TGG | 0.603633 | 2.2:-2155327 | MS.gene36339:CDS |
| GTAGAAATGACAAGCAGTTG+TGG | 0.607396 | 2.2:-2154274 | MS.gene36339:CDS |
| TGTACTAACAATCCATTTGT+GGG | 0.623733 | 2.2:+2154783 | None:intergenic |
| TATCATGGAACAGAAATCCA+GGG | 0.639144 | 2.2:-2153097 | MS.gene36339:CDS |
| AGGATAGTCCACCAACAACA+AGG | 0.647718 | 2.2:+2153471 | None:intergenic |
| ACTGCATCTTTAGCATCAGA+GGG | 0.648958 | 2.2:+2154844 | None:intergenic |
| GATCCAGTAGACAAAACAGA+AGG | 0.655095 | 2.2:-2154218 | MS.gene36339:CDS |
| TGAACATACTCCATGCAATG+AGG | 0.658438 | 2.2:+2154171 | None:intergenic |
| AAGGAGCCAATTGTTCCTTG+TGG | 0.669710 | 2.2:-2154194 | MS.gene36339:CDS |
| ACACATACGAGTTTCAAGGT+AGG | 0.677272 | 2.2:-2153559 | MS.gene36339:CDS |
| TGTGGCGTAAGTCAGCTGAA+GGG | 0.686602 | 2.2:-2154256 | MS.gene36339:CDS |
| AAGTAGGATGGATCCCCAAG+AGG | 0.710797 | 2.2:+2153119 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTGTTTTTTTTTTTTTTTTT+TGG | + | chr2.2:2153275-2153294 | None:intergenic | 10.0% |
| !!! | TTTAGACAAAAATTATATTT+TGG | + | chr2.2:2153576-2153595 | None:intergenic | 10.0% |
| !! | ACTAATTATTATTTCCATTT+AGG | + | chr2.2:2154052-2154071 | None:intergenic | 15.0% |
| !! | ATTAAAATCTTGGAAAATTT+AGG | - | chr2.2:2153329-2153348 | MS.gene36339:intron | 15.0% |
| !! | TTAAAATCTTGGAAAATTTA+GGG | - | chr2.2:2153330-2153349 | MS.gene36339:intron | 15.0% |
| !! | TTTATGTTAGGAAATTAATA+TGG | - | chr2.2:2155539-2155558 | MS.gene36339:intron | 15.0% |
| !!! | CAACTGAATTTTATTTATAA+TGG | - | chr2.2:2153840-2153859 | MS.gene36339:intron | 15.0% |
| !!! | TATAATTTTTGTCTAAATCA+CGG | - | chr2.2:2153579-2153598 | MS.gene36339:CDS | 15.0% |
| !! | AACAAAAACAAATTTACTTG+AGG | - | chr2.2:2154373-2154392 | MS.gene36339:intron | 20.0% |
| !! | AATTTCAGTGTTATCAATTA+CGG | - | chr2.2:2154165-2154184 | MS.gene36339:CDS | 20.0% |
| !! | AGTGACAAAAATTCTAAATT+TGG | - | chr2.2:2154688-2154707 | MS.gene36339:intron | 20.0% |
| !! | ATCATCCAAAATCATATTAA+AGG | + | chr2.2:2154451-2154470 | None:intergenic | 20.0% |
| !! | ATTAAACTGTTTATCCAAAT+AGG | + | chr2.2:2154794-2154813 | None:intergenic | 20.0% |
| !! | GTTCTGATAAAATTTATGTT+AGG | - | chr2.2:2155527-2155546 | MS.gene36339:intron | 20.0% |
| !! | TATTAAGAAACTGTAAGAAA+TGG | + | chr2.2:2153651-2153670 | None:intergenic | 20.0% |
| !! | TTAAACTGTTTATCCAAATA+GGG | + | chr2.2:2154793-2154812 | None:intergenic | 20.0% |
| !!! | ATTTTCCAAGATTTTAATAC+AGG | + | chr2.2:2153327-2153346 | None:intergenic | 20.0% |
| !!! | TTTATGTCTGTAATTTTATC+AGG | - | chr2.2:2153694-2153713 | MS.gene36339:CDS | 20.0% |
| !!! | TTTTCATTTTCATACCTAAA+TGG | - | chr2.2:2154035-2154054 | MS.gene36339:CDS | 20.0% |
| ! | GCAAATTAAGACATACATTT+AGG | + | chr2.2:2153154-2153173 | None:intergenic | 25.0% |
| ! | GTTTATACGTAAGTACTTAT+AGG | + | chr2.2:2154832-2154851 | None:intergenic | 25.0% |
| ! | TAATTAGTTGCAAACTTTAC+AGG | - | chr2.2:2154064-2154083 | MS.gene36339:CDS | 25.0% |
| !! | AAGAAAAGCTGAAACATTTT+TGG | - | chr2.2:2155572-2155591 | MS.gene36339:intron | 25.0% |
| !! | TATCCTAAAAATTTTCAGAG+TGG | - | chr2.2:2154721-2154740 | MS.gene36339:intron | 25.0% |
| !! | TGATGCCTTTAATATGATTT+TGG | - | chr2.2:2154443-2154462 | MS.gene36339:intron | 25.0% |
| !! | TTGAATAAGCTCTTTTCTAT+TGG | - | chr2.2:2155491-2155510 | MS.gene36339:intron | 25.0% |
| !! | TTGGAAGTTTTCTTTGAATT+TGG | + | chr2.2:2153127-2153146 | None:intergenic | 25.0% |
| !! | TTTGATAGTAATTATCAAGC+TGG | + | chr2.2:2154127-2154146 | None:intergenic | 25.0% |
| !!! | ATTGTTTCAAGTTACATCAA+AGG | + | chr2.2:2153241-2153260 | None:intergenic | 25.0% |
| !!! | GCTTTTTCTATACTACTTTA+TGG | - | chr2.2:2155382-2155401 | MS.gene36339:intron | 25.0% |
| AACAAAAAGAACATAGCATG+GGG | - | chr2.2:2154664-2154683 | MS.gene36339:CDS | 30.0% | |
| ACATCAAAGGAAGAAAAAGA+AGG | + | chr2.2:2153228-2153247 | None:intergenic | 30.0% | |
| ATGTACTAACAATCCATTTG+TGG | + | chr2.2:2153989-2154008 | None:intergenic | 30.0% | |
| ATTACTATCAAAATCATCGC+CGG | - | chr2.2:2154135-2154154 | MS.gene36339:CDS | 30.0% | |
| ATTTCTGTTCCATGATAAGT+AGG | + | chr2.2:2155668-2155687 | None:intergenic | 30.0% | |
| CAACAAAAAGAACATAGCAT+GGG | - | chr2.2:2154663-2154682 | MS.gene36339:CDS | 30.0% | |
| CAACTTTCAGAAAACTGTAT+GGG | - | chr2.2:2155125-2155144 | MS.gene36339:intron | 30.0% | |
| CATTTGTTCCTTGTGATTTA+CGG | - | chr2.2:2153299-2153318 | MS.gene36339:intron | 30.0% | |
| GAAGTGCATGCTTAAATTAA+CGG | + | chr2.2:2153629-2153648 | None:intergenic | 30.0% | |
| GATATGACTTCATCAAAAGA+TGG | - | chr2.2:2153091-2153110 | MS.gene36339:CDS | 30.0% | |
| GCAAAATATTTCCTTTACCT+TGG | + | chr2.2:2155364-2155383 | None:intergenic | 30.0% | |
| GTTGTCCATTGAAAATTTGT+AGG | + | chr2.2:2154629-2154648 | None:intergenic | 30.0% | |
| TAAAGAAATACTAATGCAGG+TGG | - | chr2.2:2153863-2153882 | MS.gene36339:intron | 30.0% | |
| TCAACAAAAAGAACATAGCA+TGG | - | chr2.2:2154662-2154681 | MS.gene36339:CDS | 30.0% | |
| TGGTAAAGAAATACTAATGC+AGG | - | chr2.2:2153860-2153879 | MS.gene36339:intron | 30.0% | |
| TGTACTAACAATCCATTTGT+GGG | + | chr2.2:2153988-2154007 | None:intergenic | 30.0% | |
| TTTCCATAAGCTCTTTCAAA+CGG | - | chr2.2:2154952-2154971 | MS.gene36339:intron | 30.0% | |
| ! | AATTTTTGTCTAAATCACGG+TGG | - | chr2.2:2153582-2153601 | MS.gene36339:CDS | 30.0% |
| ! | AGACATACATTTAGGTTTCT+TGG | + | chr2.2:2153146-2153165 | None:intergenic | 30.0% |
| ! | CAAATTTTCAATGGACAACA+AGG | - | chr2.2:2154630-2154649 | MS.gene36339:intron | 30.0% |
| ! | GAAGTTTTCTTTGAATTTGG+AGG | + | chr2.2:2153124-2153143 | None:intergenic | 30.0% |
| ! | TGACACCTACAAATTTTCAA+TGG | - | chr2.2:2154621-2154640 | MS.gene36339:intron | 30.0% |
| ! | TTGCATCATTGTTTTCATCA+GGG | - | chr2.2:2153765-2153784 | MS.gene36339:intron | 30.0% |
| AACAAGAGGATCTGATTGTT+TGG | - | chr2.2:2155083-2155102 | MS.gene36339:intron | 35.0% | |
| AACAATGATGCAAGACCAAT+AGG | + | chr2.2:2153758-2153777 | None:intergenic | 35.0% | |
| AACACTGAAATTGTGCATAC+CGG | + | chr2.2:2154157-2154176 | None:intergenic | 35.0% | |
| ACAATGATGCAAGACCAATA+GGG | + | chr2.2:2153757-2153776 | None:intergenic | 35.0% | |
| AGCTGGTAGTAGATAAAAAC+AGG | + | chr2.2:2154110-2154129 | None:intergenic | 35.0% | |
| AGTAGACAAAACAGAAGGAA+AGG | - | chr2.2:2154555-2154574 | MS.gene36339:intron | 35.0% | |
| ATTGCCACATTTACTGCAAT+TGG | - | chr2.2:2153719-2153738 | MS.gene36339:intron | 35.0% | |
| CCAACTTTCAGAAAACTGTA+TGG | - | chr2.2:2155124-2155143 | MS.gene36339:intron | 35.0% | |
| CGTAAATCACAAGGAACAAA+TGG | + | chr2.2:2153301-2153320 | None:intergenic | 35.0% | |
| CTATCAACTACAACTTGGAT+TGG | - | chr2.2:2155244-2155263 | MS.gene36339:intron | 35.0% | |
| GATTCCAATTGCAGTAAATG+TGG | + | chr2.2:2153726-2153745 | None:intergenic | 35.0% | |
| GGAATCATCTTTATCCCTAT+TGG | - | chr2.2:2153740-2153759 | MS.gene36339:intron | 35.0% | |
| GGTGGGAAAAATAGATTCTT+GGG | - | chr2.2:2155265-2155284 | MS.gene36339:intron | 35.0% | |
| GTGGGAAAAATAGATTCTTG+GGG | - | chr2.2:2155266-2155285 | MS.gene36339:intron | 35.0% | |
| GTGTTATCAATTACGGATCA+CGG | - | chr2.2:2154172-2154191 | MS.gene36339:CDS | 35.0% | |
| TACAACACATACGAGTTTCA+AGG | - | chr2.2:2155205-2155224 | MS.gene36339:intron | 35.0% | |
| TATCATGGAACAGAAATCCA+GGG | - | chr2.2:2155671-2155690 | MS.gene36339:CDS | 35.0% | |
| TGGTGGGAAAAATAGATTCT+TGG | - | chr2.2:2155264-2155283 | MS.gene36339:intron | 35.0% | |
| TTATCATGGAACAGAAATCC+AGG | - | chr2.2:2155670-2155689 | MS.gene36339:CDS | 35.0% | |
| TTATCCAAATAGGGACTTAC+AGG | + | chr2.2:2154784-2154803 | None:intergenic | 35.0% | |
| ! | ATCATTGTTTTCATCAGGGA+AGG | - | chr2.2:2153769-2153788 | MS.gene36339:intron | 35.0% |
| ! | CCTAAAAATTTTCAGAGTGG+AGG | - | chr2.2:2154724-2154743 | MS.gene36339:intron | 35.0% |
| ! | CTTGCATCATTGTTTTCATC+AGG | - | chr2.2:2153764-2153783 | MS.gene36339:intron | 35.0% |
| ! | TGGTTCTATCAACTACAACT+TGG | - | chr2.2:2155239-2155258 | MS.gene36339:intron | 35.0% |
| ! | TTAGTGCATGTTTGAAACCA+CGG | - | chr2.2:2153515-2153534 | MS.gene36339:CDS | 35.0% |
| ! | TTTCCTTCTGTTTTGTCTAC+TGG | + | chr2.2:2154556-2154575 | None:intergenic | 35.0% |
| !! | CCATACAGTTTTCTGAAAGT+TGG | + | chr2.2:2155127-2155146 | None:intergenic | 35.0% |
| !! | CCTCCACTCTGAAAATTTTT+AGG | + | chr2.2:2154727-2154746 | None:intergenic | 35.0% |
| AACCAATTCTAACACCTGGA+TGG | - | chr2.2:2153465-2153484 | MS.gene36339:CDS | 40.0% | |
| AACTGCATCTTTAGCATCAG+AGG | + | chr2.2:2153928-2153947 | None:intergenic | 40.0% | |
| ACACATACGAGTTTCAAGGT+AGG | - | chr2.2:2155209-2155228 | MS.gene36339:intron | 40.0% | |
| ACCAATTCTAACACCTGGAT+GGG | - | chr2.2:2153466-2153485 | MS.gene36339:CDS | 40.0% | |
| ACTGCATCTTTAGCATCAGA+GGG | + | chr2.2:2153927-2153946 | None:intergenic | 40.0% | |
| AGAAATACTAATGCAGGTGG+TGG | - | chr2.2:2153866-2153885 | MS.gene36339:intron | 40.0% | |
| ATGTGATCAAGCCAAGGTAA+AGG | - | chr2.2:2155350-2155369 | MS.gene36339:intron | 40.0% | |
| CATACCTGTAAGTCCCTATT+TGG | - | chr2.2:2154777-2154796 | MS.gene36339:intron | 40.0% | |
| CGGAGCCTGTATTAAAATCT+TGG | - | chr2.2:2153319-2153338 | MS.gene36339:intron | 40.0% | |
| CTATCCTTGTTCTGTGCTAT+AGG | - | chr2.2:2155313-2155332 | MS.gene36339:CDS | 40.0% | |
| CTGTTCCATGATAAGTAGGA+TGG | + | chr2.2:2155664-2155683 | None:intergenic | 40.0% | |
| GAATCCTATAGCACAGAACA+AGG | + | chr2.2:2155320-2155339 | None:intergenic | 40.0% | |
| GATCCAGTAGACAAAACAGA+AGG | - | chr2.2:2154550-2154569 | MS.gene36339:intron | 40.0% | |
| GTAGAAATGACAAGCAGTTG+TGG | - | chr2.2:2154494-2154513 | MS.gene36339:intron | 40.0% | |
| GTTGCATATTATACCCATCC+AGG | + | chr2.2:2153482-2153501 | None:intergenic | 40.0% | |
| GTTTCAAGGTAGGAAGAAGT+TGG | - | chr2.2:2155219-2155238 | MS.gene36339:intron | 40.0% | |
| TCATTGAAAATCGTGCTGTG+TGG | + | chr2.2:2154353-2154372 | None:intergenic | 40.0% | |
| TGAACATACTCCATGCAATG+AGG | + | chr2.2:2154600-2154619 | None:intergenic | 40.0% | |
| TGACCGTTTGAAAGAGCTTA+TGG | + | chr2.2:2154958-2154977 | None:intergenic | 40.0% | |
| TGGAAACCAATTCTAACACC+TGG | - | chr2.2:2153461-2153480 | MS.gene36339:CDS | 40.0% | |
| TTCTGTATGTGATCAAGCCA+AGG | - | chr2.2:2155344-2155363 | MS.gene36339:intron | 40.0% | |
| TTTCAGAGTGGAGGTTTGAT+TGG | - | chr2.2:2154733-2154752 | MS.gene36339:intron | 40.0% | |
| ! | CTTGGAAAATTTAGGGCTTG+AGG | - | chr2.2:2153337-2153356 | MS.gene36339:intron | 40.0% |
| ! | GTTAGAATTGGTTTCCATGC+AGG | + | chr2.2:2153458-2153477 | None:intergenic | 40.0% |
| ! | TCAACTACAACTTGGATTGG+TGG | - | chr2.2:2155247-2155266 | MS.gene36339:intron | 40.0% |
| ! | TTTTGTCTACTGGATCACAG+TGG | + | chr2.2:2154546-2154565 | None:intergenic | 40.0% |
| !! | ATTGCATACCTTGTTGTTGG+TGG | - | chr2.2:2155289-2155308 | MS.gene36339:intron | 40.0% |
| !! | CAACTACAACTTGGATTGGT+GGG | - | chr2.2:2155248-2155267 | MS.gene36339:intron | 40.0% |
| AAGGAGCCAATTGTTCCTTG+TGG | - | chr2.2:2154574-2154593 | MS.gene36339:intron | 45.0% | |
| ACAAGACTTGCACCCACAAA+TGG | - | chr2.2:2153973-2153992 | MS.gene36339:intron | 45.0% | |
| ACCCATCCAGGTGTTAGAAT+TGG | + | chr2.2:2153470-2153489 | None:intergenic | 45.0% | |
| AGGATAGTCCACCAACAACA+AGG | + | chr2.2:2155300-2155319 | None:intergenic | 45.0% | |
| ATGAGGCCACAAGGAACAAT+TGG | + | chr2.2:2154583-2154602 | None:intergenic | 45.0% | |
| GCATCAGTTGAGTGAACAAG+AGG | - | chr2.2:2155069-2155088 | MS.gene36339:intron | 45.0% | |
| GGGATCCATCCTACTTATCA+TGG | - | chr2.2:2155656-2155675 | MS.gene36339:CDS | 45.0% | |
| TACAGGCTCCGTAAATCACA+AGG | + | chr2.2:2153310-2153329 | None:intergenic | 45.0% | |
| TGATATTGTCAAACTCGCCG+TGG | + | chr2.2:2153535-2153554 | None:intergenic | 45.0% | |
| ! | CTCTTTTTGCAGACCTCTTG+GGG | - | chr2.2:2155636-2155655 | MS.gene36339:CDS | 45.0% |
| ! | GCTCTTTTTGCAGACCTCTT+GGG | - | chr2.2:2155635-2155654 | MS.gene36339:CDS | 45.0% |
| ! | GGGATTGCATACCTTGTTGT+TGG | - | chr2.2:2155286-2155305 | MS.gene36339:intron | 45.0% |
| ! | TGCTCTTTTTGCAGACCTCT+TGG | - | chr2.2:2155634-2155653 | MS.gene36339:CDS | 45.0% |
| AAGTAGGATGGATCCCCAAG+AGG | + | chr2.2:2155652-2155671 | None:intergenic | 50.0% | |
| CACAGCAAGAACTACCTGCA+TGG | - | chr2.2:2153441-2153460 | MS.gene36339:CDS | 50.0% | |
| CAGAGTGGAGGTTTGATTGG+AGG | - | chr2.2:2154736-2154755 | MS.gene36339:intron | 50.0% | |
| TGTGGCGTAAGTCAGCTGAA+GGG | - | chr2.2:2154512-2154531 | MS.gene36339:intron | 50.0% | |
| TTCCTTGTGGCCTCATTGCA+TGG | - | chr2.2:2154587-2154606 | MS.gene36339:intron | 50.0% | |
| TTGTGGCGTAAGTCAGCTGA+AGG | - | chr2.2:2154511-2154530 | MS.gene36339:intron | 50.0% | |
| !! | GTGGGTGCAAGTCTTGTTAG+TGG | + | chr2.2:2153970-2153989 | None:intergenic | 50.0% |
| CTCCATGCAATGAGGCCACA+AGG | + | chr2.2:2154592-2154611 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.2 | gene | 2153085 | 2155705 | 2153085 | ID=MS.gene36339 |
| chr2.2 | mRNA | 2153085 | 2155705 | 2153085 | ID=MS.gene36339.t1;Parent=MS.gene36339 |
| chr2.2 | exon | 2155633 | 2155705 | 2155633 | ID=MS.gene36339.t1.exon1;Parent=MS.gene36339.t1 |
| chr2.2 | CDS | 2155633 | 2155705 | 2155633 | ID=cds.MS.gene36339.t1;Parent=MS.gene36339.t1 |
| chr2.2 | exon | 2155303 | 2155361 | 2155303 | ID=MS.gene36339.t1.exon2;Parent=MS.gene36339.t1 |
| chr2.2 | CDS | 2155303 | 2155361 | 2155303 | ID=cds.MS.gene36339.t1;Parent=MS.gene36339.t1 |
| chr2.2 | exon | 2155000 | 2155074 | 2155000 | ID=MS.gene36339.t1.exon3;Parent=MS.gene36339.t1 |
| chr2.2 | CDS | 2155000 | 2155074 | 2155000 | ID=cds.MS.gene36339.t1;Parent=MS.gene36339.t1 |
| chr2.2 | exon | 2154792 | 2154908 | 2154792 | ID=MS.gene36339.t1.exon4;Parent=MS.gene36339.t1 |
| chr2.2 | CDS | 2154792 | 2154908 | 2154792 | ID=cds.MS.gene36339.t1;Parent=MS.gene36339.t1 |
| chr2.2 | exon | 2154634 | 2154704 | 2154634 | ID=MS.gene36339.t1.exon5;Parent=MS.gene36339.t1 |
| chr2.2 | CDS | 2154634 | 2154704 | 2154634 | ID=cds.MS.gene36339.t1;Parent=MS.gene36339.t1 |
| chr2.2 | exon | 2154007 | 2154307 | 2154007 | ID=MS.gene36339.t1.exon6;Parent=MS.gene36339.t1 |
| chr2.2 | CDS | 2154007 | 2154307 | 2154007 | ID=cds.MS.gene36339.t1;Parent=MS.gene36339.t1 |
| chr2.2 | exon | 2153425 | 2153714 | 2153425 | ID=MS.gene36339.t1.exon7;Parent=MS.gene36339.t1 |
| chr2.2 | CDS | 2153425 | 2153714 | 2153425 | ID=cds.MS.gene36339.t1;Parent=MS.gene36339.t1 |
| chr2.2 | exon | 2153085 | 2153142 | 2153085 | ID=MS.gene36339.t1.exon8;Parent=MS.gene36339.t1 |
| chr2.2 | CDS | 2153085 | 2153142 | 2153085 | ID=cds.MS.gene36339.t1;Parent=MS.gene36339.t1 |
| Gene Sequence |
| Protein sequence |
