AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84086


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84086.t1 MTR_1g051205 98.104 211 4 0 1 211 188 398 4.93e-157 440
MS.gene84086.t1 MTR_0004s0610 96.682 211 7 0 1 211 189 399 5.20e-154 432
MS.gene84086.t1 MTR_5g068170 81.429 210 22 1 1 210 118 310 1.04e-113 327
MS.gene84086.t1 MTR_6g034745 78.723 47 10 0 108 154 271 317 4.46e-19 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84086.t1 AT3G42630 66.825 211 69 1 1 211 206 415 8.49e-104 305
MS.gene84086.t1 AT4G14190 29.384 211 135 5 1 201 291 497 3.19e-13 68.2

Find 47 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTTACGACGTTTAATATT+AGG 0.162570 1.4:+37655663 MS.gene84086:CDS
ATAGGAGGAACCAGATATTT+TGG 0.193003 1.4:+37656011 MS.gene84086:CDS
TAGAAAGCTTGGAAGGAATT+TGG 0.211995 1.4:+37655808 MS.gene84086:CDS
TTTCGAGGATGGCGCTGTTT+TGG 0.262354 1.4:+37655696 MS.gene84086:CDS
GAAGAGAAAATTCTATGAAT+TGG 0.262799 1.4:+37655493 MS.gene84086:CDS
GTTAATAGTTCCAAAATATC+TGG 0.282061 1.4:-37656021 None:intergenic
AACAACAGAAATGGAGTTAT+AGG 0.284479 1.4:+37655957 MS.gene84086:CDS
GCTTGCAGAGAGAGTTTGTT+AGG 0.289369 1.4:+37655615 MS.gene84086:CDS
TGTTACGACGTTTAATATTA+GGG 0.297675 1.4:+37655664 MS.gene84086:CDS
AATGAAATGGAAAATGCTTA+TGG 0.317533 1.4:+37655401 None:intergenic
GTGATTAGGGCTATGGCTTA+TGG 0.328397 1.4:+37655461 MS.gene84086:CDS
GATGGAGGAAAATGTGATTA+GGG 0.335950 1.4:+37655448 MS.gene84086:CDS
TGAAAAGGTTGTTCCTGATT+TGG 0.336453 1.4:+37655751 MS.gene84086:CDS
TGATGGAGGAAAATGTGATT+AGG 0.338371 1.4:+37655447 MS.gene84086:CDS
TACAGATCCGTTTGTGTTTG+AGG 0.346471 1.4:+37655877 MS.gene84086:CDS
ACAACAGAAATGGAGTTATA+GGG 0.350987 1.4:+37655958 MS.gene84086:CDS
TTTGTTAGGATGGTTGAATC+AGG 0.352533 1.4:+37655629 MS.gene84086:CDS
ATGGAAGGTTTAAGAGATCT+AGG 0.371453 1.4:+37655420 MS.gene84086:CDS
AATATCTTAAGAAACATTAT+AGG 0.375645 1.4:+37655993 MS.gene84086:CDS
GGTTTAGGTAGGAGAAATGT+TGG 0.378690 1.4:+37655536 MS.gene84086:CDS
AAGAGAAAATTCTATGAATT+GGG 0.388525 1.4:+37655494 MS.gene84086:CDS
TTTGTTAGAGATGTTGGTTT+AGG 0.396561 1.4:+37655521 MS.gene84086:CDS
GTTCCTGATTTGGTTACTTA+CGG 0.403448 1.4:+37655761 MS.gene84086:CDS
GGTGAGTTTGTTAGAGATGT+TGG 0.404844 1.4:+37655515 MS.gene84086:CDS
ACAAGACACAACAACAGAAA+TGG 0.420204 1.4:+37655948 MS.gene84086:CDS
CAACCGTAAGTAACCAAATC+AGG 0.433446 1.4:-37655764 None:intergenic
ATCTTAGTATTGAACATATG+AGG 0.444340 1.4:+37655726 MS.gene84086:CDS
AGGTGACTTTCATTTGAGTT+CGG 0.445273 1.4:+37655910 MS.gene84086:CDS
GACGTTTAATATTAGGGCTA+TGG 0.449092 1.4:+37655670 MS.gene84086:CDS
TTGTGTTGTGGAGGCTTACT+TGG 0.462364 1.4:+37655784 MS.gene84086:CDS
GCTTACTTGGATAGAAAGCT+TGG 0.481941 1.4:+37655797 MS.gene84086:CDS
GGTTGAATCAGGGTTTCGTC+CGG 0.489825 1.4:+37655640 MS.gene84086:CDS
GCAGAGAGAGTTTGTTAGGA+TGG 0.492223 1.4:+37655619 MS.gene84086:CDS
GTTTAGGTAGGAGAAATGTT+GGG 0.493418 1.4:+37655537 MS.gene84086:CDS
ATATTAAACGTCGTAACATC+CGG 0.502209 1.4:-37655659 None:intergenic
TTAGAGATGTTGGTTTAGGT+AGG 0.503038 1.4:+37655525 MS.gene84086:CDS
GGAAAATGTGATTAGGGCTA+TGG 0.512451 1.4:+37655454 MS.gene84086:CDS
TTGTTAGGATGGTTGAATCA+GGG 0.518440 1.4:+37655630 MS.gene84086:CDS
TGAACATATGAGGAGTGAAA+AGG 0.526259 1.4:+37655736 MS.gene84086:CDS
CCTAAAACCTCAAACACAAA+CGG 0.528045 1.4:-37655884 None:intergenic
AAATGGAAAATGCTTATGGA+AGG 0.541034 1.4:+37655405 None:intergenic
ACTTGGATAGAAAGCTTGGA+AGG 0.554688 1.4:+37655801 MS.gene84086:CDS
AACGGATCTGTAAACAATCT+CGG 0.566471 1.4:-37655866 None:intergenic
TGACTTTCATTTGAGTTCGG+AGG 0.582232 1.4:+37655913 MS.gene84086:CDS
GGTTACTTACGGTTGTGTTG+TGG 0.592231 1.4:+37655772 MS.gene84086:CDS
TACTTACGGTTGTGTTGTGG+AGG 0.598880 1.4:+37655775 MS.gene84086:CDS
ATCTTAAGAAACATTATAGG+AGG 0.601959 1.4:+37655996 MS.gene84086:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATCTTAAGAAACATTAT+AGG + chr1.4:37655993-37656012 MS.gene84086:CDS 15.0%
!! AAGAGAAAATTCTATGAATT+GGG + chr1.4:37655494-37655513 MS.gene84086:CDS 20.0%
! ATCTTAAGAAACATTATAGG+AGG + chr1.4:37655996-37656015 MS.gene84086:CDS 25.0%
! ATGTTACGACGTTTAATATT+AGG + chr1.4:37655663-37655682 MS.gene84086:CDS 25.0%
! GAAGAGAAAATTCTATGAAT+TGG + chr1.4:37655493-37655512 MS.gene84086:CDS 25.0%
! TGTTACGACGTTTAATATTA+GGG + chr1.4:37655664-37655683 MS.gene84086:CDS 25.0%
!! ATCTTAGTATTGAACATATG+AGG + chr1.4:37655726-37655745 MS.gene84086:CDS 25.0%
!!! GGAGTTTGTTTTGAATAAAA+TGG + chr1.4:37655829-37655848 MS.gene84086:CDS 25.0%
!!! TGTTTTGAATAAAATGGATG+TGG + chr1.4:37655835-37655854 MS.gene84086:CDS 25.0%
AACAACAGAAATGGAGTTAT+AGG + chr1.4:37655957-37655976 MS.gene84086:CDS 30.0%
ACAACAGAAATGGAGTTATA+GGG + chr1.4:37655958-37655977 MS.gene84086:CDS 30.0%
ATATTAAACGTCGTAACATC+CGG - chr1.4:37655662-37655681 None:intergenic 30.0%
! TAAGAGATCTAGGTTTTTGA+TGG + chr1.4:37655430-37655449 MS.gene84086:CDS 30.0%
!! TTTGTTAGAGATGTTGGTTT+AGG + chr1.4:37655521-37655540 MS.gene84086:CDS 30.0%
AACGGATCTGTAAACAATCT+CGG - chr1.4:37655869-37655888 None:intergenic 35.0%
ACAAGACACAACAACAGAAA+TGG + chr1.4:37655948-37655967 MS.gene84086:CDS 35.0%
ATGGAAGGTTTAAGAGATCT+AGG + chr1.4:37655420-37655439 MS.gene84086:CDS 35.0%
CCTAAAACCTCAAACACAAA+CGG - chr1.4:37655887-37655906 None:intergenic 35.0%
GACGTTTAATATTAGGGCTA+TGG + chr1.4:37655670-37655689 MS.gene84086:CDS 35.0%
GATGGAGGAAAATGTGATTA+GGG + chr1.4:37655448-37655467 MS.gene84086:CDS 35.0%
GTTTAGGTAGGAGAAATGTT+GGG + chr1.4:37655537-37655556 MS.gene84086:CDS 35.0%
TAGAAAGCTTGGAAGGAATT+TGG + chr1.4:37655808-37655827 MS.gene84086:CDS 35.0%
TGAAAAGGTTGTTCCTGATT+TGG + chr1.4:37655751-37655770 MS.gene84086:CDS 35.0%
TGAACATATGAGGAGTGAAA+AGG + chr1.4:37655736-37655755 MS.gene84086:CDS 35.0%
TGATGGAGGAAAATGTGATT+AGG + chr1.4:37655447-37655466 MS.gene84086:CDS 35.0%
TTGTTAGGATGGTTGAATCA+GGG + chr1.4:37655630-37655649 MS.gene84086:CDS 35.0%
TTTGTTAGGATGGTTGAATC+AGG + chr1.4:37655629-37655648 MS.gene84086:CDS 35.0%
! AGGTGACTTTCATTTGAGTT+CGG + chr1.4:37655910-37655929 MS.gene84086:CDS 35.0%
! ATAGGAGGAACCAGATATTT+TGG + chr1.4:37656011-37656030 MS.gene84086:CDS 35.0%
! GAAATGTTGGGAATCTTTTG+TGG + chr1.4:37655549-37655568 MS.gene84086:CDS 35.0%
!! GTTCCTGATTTGGTTACTTA+CGG + chr1.4:37655761-37655780 MS.gene84086:CDS 35.0%
!! TTAGAGATGTTGGTTTAGGT+AGG + chr1.4:37655525-37655544 MS.gene84086:CDS 35.0%
!!! GTGTTTGAGGTTTTAGGTAA+AGG + chr1.4:37655890-37655909 MS.gene84086:CDS 35.0%
ACTTGGATAGAAAGCTTGGA+AGG + chr1.4:37655801-37655820 MS.gene84086:CDS 40.0%
CAACCGTAAGTAACCAAATC+AGG - chr1.4:37655767-37655786 None:intergenic 40.0%
GCTTACTTGGATAGAAAGCT+TGG + chr1.4:37655797-37655816 MS.gene84086:CDS 40.0%
GGAAAATGTGATTAGGGCTA+TGG + chr1.4:37655454-37655473 MS.gene84086:CDS 40.0%
GGTTTAGGTAGGAGAAATGT+TGG + chr1.4:37655536-37655555 MS.gene84086:CDS 40.0%
TACAGATCCGTTTGTGTTTG+AGG + chr1.4:37655877-37655896 MS.gene84086:CDS 40.0%
! GAGATCTAGGTTTTTGATGG+AGG + chr1.4:37655433-37655452 MS.gene84086:CDS 40.0%
! TGACTTTCATTTGAGTTCGG+AGG + chr1.4:37655913-37655932 MS.gene84086:CDS 40.0%
!! CCGTTTGTGTTTGAGGTTTT+AGG + chr1.4:37655884-37655903 MS.gene84086:CDS 40.0%
!! GGTGAGTTTGTTAGAGATGT+TGG + chr1.4:37655515-37655534 MS.gene84086:CDS 40.0%
GGTTACTTACGGTTGTGTTG+TGG + chr1.4:37655772-37655791 MS.gene84086:CDS 45.0%
GTGATTAGGGCTATGGCTTA+TGG + chr1.4:37655461-37655480 MS.gene84086:CDS 45.0%
TACTTACGGTTGTGTTGTGG+AGG + chr1.4:37655775-37655794 MS.gene84086:CDS 45.0%
TTGTGTTGTGGAGGCTTACT+TGG + chr1.4:37655784-37655803 MS.gene84086:CDS 45.0%
! GCTTGCAGAGAGAGTTTGTT+AGG + chr1.4:37655615-37655634 MS.gene84086:CDS 45.0%
!! GCAGAGAGAGTTTGTTAGGA+TGG + chr1.4:37655619-37655638 MS.gene84086:CDS 45.0%
!!! TTTGAGTTCGGAGGCGTTTT+TGG + chr1.4:37655922-37655941 MS.gene84086:CDS 45.0%
GGTTGAATCAGGGTTTCGTC+CGG + chr1.4:37655640-37655659 MS.gene84086:CDS 50.0%
! TTAGGGCTATGGCGTTTTCG+AGG + chr1.4:37655681-37655700 MS.gene84086:CDS 50.0%
! TTTCGAGGATGGCGCTGTTT+TGG + chr1.4:37655696-37655715 MS.gene84086:CDS 50.0%
!! TTCGAGGATGGCGCTGTTTT+GGG + chr1.4:37655697-37655716 MS.gene84086:CDS 50.0%
!! GGCTATGGCGTTTTCGAGGA+TGG + chr1.4:37655685-37655704 MS.gene84086:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 37655407 37656042 37655407 ID=MS.gene84086
chr1.4 mRNA 37655407 37656042 37655407 ID=MS.gene84086.t1;Parent=MS.gene84086
chr1.4 exon 37655407 37656042 37655407 ID=MS.gene84086.t1.exon1;Parent=MS.gene84086.t1
chr1.4 CDS 37655407 37656042 37655407 ID=cds.MS.gene84086.t1;Parent=MS.gene84086.t1
Gene Sequence

>MS.gene84086

ATGGAAAATGCTTATGGAAGGTTTAAGAGATCTAGGTTTTTGATGGAGGAAAATGTGATTAGGGCTATGGCTTATGGTTATACAAAGAAGAGAAAATTCTATGAATTGGGTGAGTTTGTTAGAGATGTTGGTTTAGGTAGGAGAAATGTTGGGAATCTTTTGTGGAATCTTCTGTTGCTTTCTTATGCTGCTAATTTTAAGATGAAGAGCTTGCAGAGAGAGTTTGTTAGGATGGTTGAATCAGGGTTTCGTCCGGATGTTACGACGTTTAATATTAGGGCTATGGCGTTTTCGAGGATGGCGCTGTTTTGGGATCTTCATCTTAGTATTGAACATATGAGGAGTGAAAAGGTTGTTCCTGATTTGGTTACTTACGGTTGTGTTGTGGAGGCTTACTTGGATAGAAAGCTTGGAAGGAATTTGGAGTTTGTTTTGAATAAAATGGATGTGGATGATTGTCCGAGATTGTTTACAGATCCGTTTGTGTTTGAGGTTTTAGGTAAAGGTGACTTTCATTTGAGTTCGGAGGCGTTTTTGGAATACAAGACACAACAACAGAAATGGAGTTATAGGGTTTTGATACAGAAATATCTTAAGAAACATTATAGGAGGAACCAGATATTTTGGAACTATTAA

Protein sequence

>MS.gene84086.t1

MENAYGRFKRSRFLMEENVIRAMAYGYTKKRKFYELGEFVRDVGLGRRNVGNLLWNLLLLSYAANFKMKSLQREFVRMVESGFRPDVTTFNIRAMAFSRMALFWDLHLSIEHMRSEKVVPDLVTYGCVVEAYLDRKLGRNLEFVLNKMDVDDCPRLFTDPFVFEVLGKGDFHLSSEAFLEYKTQQQKWSYRVLIQKYLKKHYRRNQIFWNY