AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92717


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92717.t1 MTR_3g030860 93.333 105 7 0 1 105 1 105 5.48e-57 171
MS.gene92717.t1 MTR_3g030850 80.000 105 20 1 1 104 1 105 1.63e-51 157
MS.gene92717.t1 MTR_4g037505 68.519 108 31 3 1 105 1 108 7.07e-39 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92717.t1 AT1G51400 55.140 107 43 3 1 104 1 105 1.12e-28 100
MS.gene92717.t1 AT3G21055 54.206 107 43 4 1 105 1 103 6.92e-24 87.8
MS.gene92717.t1 AT3G21055 54.206 107 43 4 1 105 41 143 1.25e-23 88.6

Find 32 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGCTGTTTGCTCTGTTGC+TGG 0.185357 3.1:-22832357 MS.gene92717:CDS
GCCTCTGGGGTGCCTCTTCT+TGG 0.187436 3.1:+22832312 None:intergenic
CTGCTGTTTGCTCTGTTGCT+GGG 0.300217 3.1:-22832356 MS.gene92717:CDS
ATTTCTACCCTCCTTGTCAT+TGG 0.348787 3.1:+22832413 None:intergenic
GTTGGGTTAGTGACACAAAC+AGG 0.359822 3.1:+22832273 None:intergenic
CTCTGTTGCTGGGGTTGCAA+TGG 0.391387 3.1:-22832346 MS.gene92717:CDS
TACTGATGACCGGTTGGTTA+TGG 0.419612 3.1:+22832503 None:intergenic
AATGACAAGGAGGGTAGAAA+TGG 0.432550 3.1:-22832411 MS.gene92717:CDS
ACCATGACATCCTCAATAGT+TGG 0.463538 3.1:-22832534 MS.gene92717:CDS
GCTAGGATTTGTCGCTATTG+AGG 0.474783 3.1:-22832249 None:intergenic
GTTTCCCTGCCATAACCAAC+CGG 0.496175 3.1:-22832512 MS.gene92717:CDS
TGCTGTTTGCTCTGTTGCTG+GGG 0.508878 3.1:-22832355 MS.gene92717:CDS
GATGACCGGTTGGTTATGGC+AGG 0.509879 3.1:+22832507 None:intergenic
TAGCGACAAATCCTAGCAGT+TGG 0.523285 3.1:+22832255 None:intergenic
GCTGTGTTGCTACTGATGAC+CGG 0.536765 3.1:+22832493 None:intergenic
AGCGACAAATCCTAGCAGTT+GGG 0.546386 3.1:+22832256 None:intergenic
AGTGCTGTAAGAGCAGTTGA+AGG 0.554404 3.1:-22832456 MS.gene92717:CDS
ACAAGGAGGGTAGAAATGGA+AGG 0.563916 3.1:-22832407 MS.gene92717:CDS
GTGTCACTAACCCAACTGCT+AGG 0.567214 3.1:-22832266 MS.gene92717:CDS
ACCAACTATTGAGGATGTCA+TGG 0.574051 3.1:+22832533 None:intergenic
GTGCTGTAAGAGCAGTTGAA+GGG 0.585017 3.1:-22832455 MS.gene92717:CDS
TGTTGCTACTGATGACCGGT+TGG 0.591177 3.1:+22832497 None:intergenic
GGCAGGGAAACCAACTATTG+AGG 0.593922 3.1:+22832524 None:intergenic
TGCTGTAAGAGCAGTTGAAG+GGG 0.596756 3.1:-22832454 MS.gene92717:CDS
AGCAGTTGAAGGGGAAAAGA+CGG 0.619574 3.1:-22832445 MS.gene92717:CDS
GCAGATGATGAACCAAGAAG+AGG 0.660121 3.1:-22832324 MS.gene92717:CDS
ATGACCGGTTGGTTATGGCA+GGG 0.676181 3.1:+22832508 None:intergenic
TTGGGTTAGTGACACAAACA+GGG 0.691418 3.1:+22832274 None:intergenic
CAGTTGTACCAATGACAAGG+AGG 0.694597 3.1:-22832421 MS.gene92717:CDS
AGTTGTACCAATGACAAGGA+GGG 0.697533 3.1:-22832420 MS.gene92717:CDS
ACCAAGAAGAGGCACCCCAG+AGG 0.700077 3.1:-22832313 MS.gene92717:CDS
GGTCAGTTGTACCAATGACA+AGG 0.701301 3.1:-22832424 MS.gene92717:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AATGACAAGGAGGGTAGAAA+TGG - chr3.1:22832386-22832405 MS.gene92717:CDS 40.0%
ACCAACTATTGAGGATGTCA+TGG + chr3.1:22832267-22832286 None:intergenic 40.0%
ACCATGACATCCTCAATAGT+TGG - chr3.1:22832263-22832282 MS.gene92717:CDS 40.0%
AGTTGTACCAATGACAAGGA+GGG - chr3.1:22832377-22832396 MS.gene92717:CDS 40.0%
ATTTCTACCCTCCTTGTCAT+TGG + chr3.1:22832387-22832406 None:intergenic 40.0%
!! TTGGGTTAGTGACACAAACA+GGG + chr3.1:22832526-22832545 None:intergenic 40.0%
!!! AACAGGGCCATACTTTTTCT+TGG + chr3.1:22832510-22832529 None:intergenic 40.0%
!!! ATACTTTTTCTTGGCCTCTG+GGG + chr3.1:22832501-22832520 None:intergenic 40.0%
!!! CATACTTTTTCTTGGCCTCT+GGG + chr3.1:22832502-22832521 None:intergenic 40.0%
ACAAGGAGGGTAGAAATGGA+AGG - chr3.1:22832390-22832409 MS.gene92717:CDS 45.0%
AGCAGTTGAAGGGGAAAAGA+CGG - chr3.1:22832352-22832371 MS.gene92717:CDS 45.0%
AGCGACAAATCCTAGCAGTT+GGG + chr3.1:22832544-22832563 None:intergenic 45.0%
AGTGCTGTAAGAGCAGTTGA+AGG - chr3.1:22832341-22832360 MS.gene92717:CDS 45.0%
CAGTTGTACCAATGACAAGG+AGG - chr3.1:22832376-22832395 MS.gene92717:CDS 45.0%
CCAGAGGCCAAGAAAAAGTA+TGG - chr3.1:22832500-22832519 MS.gene92717:CDS 45.0%
GCAGATGATGAACCAAGAAG+AGG - chr3.1:22832473-22832492 MS.gene92717:CDS 45.0%
GGTCAGTTGTACCAATGACA+AGG - chr3.1:22832373-22832392 MS.gene92717:CDS 45.0%
GTGCTGTAAGAGCAGTTGAA+GGG - chr3.1:22832342-22832361 MS.gene92717:CDS 45.0%
TAGCGACAAATCCTAGCAGT+TGG + chr3.1:22832545-22832564 None:intergenic 45.0%
TGCTGTAAGAGCAGTTGAAG+GGG - chr3.1:22832343-22832362 MS.gene92717:CDS 45.0%
! TACTGATGACCGGTTGGTTA+TGG + chr3.1:22832297-22832316 None:intergenic 45.0%
!! GTTGGGTTAGTGACACAAAC+AGG + chr3.1:22832527-22832546 None:intergenic 45.0%
!!! CCATACTTTTTCTTGGCCTC+TGG + chr3.1:22832503-22832522 None:intergenic 45.0%
GGCAGGGAAACCAACTATTG+AGG + chr3.1:22832276-22832295 None:intergenic 50.0%
GTTTCCCTGCCATAACCAAC+CGG - chr3.1:22832285-22832304 MS.gene92717:CDS 50.0%
TGTTGCTACTGATGACCGGT+TGG + chr3.1:22832303-22832322 None:intergenic 50.0%
! ATGACCGGTTGGTTATGGCA+GGG + chr3.1:22832292-22832311 None:intergenic 50.0%
! CTGCTGTTTGCTCTGTTGCT+GGG - chr3.1:22832441-22832460 MS.gene92717:CDS 50.0%
! GCTGTGTTGCTACTGATGAC+CGG + chr3.1:22832307-22832326 None:intergenic 50.0%
! GTGTCACTAACCCAACTGCT+AGG - chr3.1:22832531-22832550 MS.gene92717:CDS 50.0%
! TGCTGTTTGCTCTGTTGCTG+GGG - chr3.1:22832442-22832461 MS.gene92717:CDS 50.0%
CTCTGTTGCTGGGGTTGCAA+TGG - chr3.1:22832451-22832470 MS.gene92717:CDS 55.0%
! GATGACCGGTTGGTTATGGC+AGG + chr3.1:22832293-22832312 None:intergenic 55.0%
! GCTGCTGTTTGCTCTGTTGC+TGG - chr3.1:22832440-22832459 MS.gene92717:CDS 55.0%
!! ACCAAGAAGAGGCACCCCAG+AGG - chr3.1:22832484-22832503 MS.gene92717:CDS 60.0%
!! GCCTCTGGGGTGCCTCTTCT+TGG + chr3.1:22832488-22832507 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 22832251 22832568 22832251 ID=MS.gene92717
chr3.1 mRNA 22832251 22832568 22832251 ID=MS.gene92717.t1;Parent=MS.gene92717
chr3.1 exon 22832251 22832568 22832251 ID=MS.gene92717.t1.exon1;Parent=MS.gene92717.t1
chr3.1 CDS 22832251 22832568 22832251 ID=cds.MS.gene92717.t1;Parent=MS.gene92717.t1
Gene Sequence

>MS.gene92717

ATGGCATCAATCACCATGACATCCTCAATAGTTGGTTTCCCTGCCATAACCAACCGGTCATCAGTAGCAACACAGCGAAGATTTGTTGTCAGTGCTGTAAGAGCAGTTGAAGGGGAAAAGACGGTCAGTTGTACCAATGACAAGGAGGGTAGAAATGGAAGGAGAGATTTGATGTTTGCTGCAGCAGCAGCTGCTGTTTGCTCTGTTGCTGGGGTTGCAATGGCAGATGATGAACCAAGAAGAGGCACCCCAGAGGCCAAGAAAAAGTATGGCCCTGTTTGTGTCACTAACCCAACTGCTAGGATTTGTCGCTATTGA

Protein sequence

>MS.gene92717.t1

MASITMTSSIVGFPAITNRSSVATQRRFVVSAVRAVEGEKTVSCTNDKEGRNGRRDLMFAAAAAAVCSVAGVAMADDEPRRGTPEAKKKYGPVCVTNPTARICRY