AlfalfaGEDB | MsG0180004294.01

M. sativa cultivar ZhongmuNo.1 / MsG0180004294.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004294.01.T01	RHN42138.1	85.202	223	22	3	1	213	25	246	2.17E-94	287
MsG0180004294.01.T01	RHN42138.1	70.408	98	27	2	13	109	172	268	3.70E-13	77.8

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004294.01.T01	A0A396GP03	85.202	223	22	3	1	213	25	246	1.04e-94	287
MsG0180004294.01.T01	G7LEV6	85.202	223	22	3	1	213	25	246	3.25e-91	291

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048058.01	MsG0180004294.01	0.814807	1.276646e-51	8.651877e-49
MsG0080048461.01	MsG0180004294.01	0.801207	1.003900e-48	4.766394e-46
MsG0080048964.01	MsG0180004294.01	0.829019	6.503736e-55	6.574464e-52
MsG0180000001.01	MsG0180004294.01	0.802439	5.606132e-49	2.746298e-46
MsG0180000101.01	MsG0180004294.01	0.803703	3.072247e-49	1.554366e-46
MsG0180000235.01	MsG0180004294.01	0.816007	6.903405e-52	4.834360e-49
MsG0180000703.01	MsG0180004294.01	0.834336	3.178661e-56	3.760846e-53
MsG0180001002.01	MsG0180004294.01	0.803311	3.703974e-49	1.855192e-46
MsG0180001294.01	MsG0180004294.01	0.832755	7.886565e-56	8.898702e-53
MsG0180001599.01	MsG0180004294.01	0.815601	8.501544e-52	5.888071e-49
MsG0180001662.01	MsG0180004294.01	0.815460	9.139049e-52	6.305050e-49
MsG0180003031.01	MsG0180004294.01	0.848940	4.447755e-60	8.296788e-57
MsG0180003304.01	MsG0180004294.01	0.805627	1.218334e-49	6.477105e-47
MsG0180003510.01	MsG0180004294.01	0.801968	7.008902e-49	3.392302e-46
MsG0180003567.01	MsG0180004294.01	0.803541	3.318205e-49	1.671982e-46
MsG0180003627.01	MsG0180004294.01	0.844844	5.876233e-59	9.608880e-56
MsG0180003807.01	MsG0180004294.01	0.801667	8.080869e-49	3.881476e-46
MsG0180003810.01	MsG0180004294.01	0.809833	1.556450e-50	9.234620e-48
MsG0180003977.01	MsG0180004294.01	0.849845	2.489790e-60	4.782933e-57
MsG0180004005.01	MsG0180004294.01	0.813618	2.336946e-51	1.533605e-48
MsG0180004050.01	MsG0180004294.01	0.824028	1.007703e-53	8.821856e-51
MsG0180004056.01	MsG0180004294.01	0.802865	4.580214e-49	2.268180e-46
MsG0180004180.01	MsG0180004294.01	0.908199	2.218095e-81	4.071745e-77
MsG0180004294.01	MsG0180004295.01	0.899457	1.945320e-77	2.408015e-73
MsG0180004294.01	MsG0180004331.01	0.847897	8.643351e-60	1.558944e-56
MsG0180004294.01	MsG0180004372.01	0.810611	1.057534e-50	6.404142e-48
MsG0180004294.01	MsG0180004389.01	0.830981	2.162217e-55	2.314824e-52
MsG0180004294.01	MsG0180004465.01	0.802772	4.787768e-49	2.365417e-46
MsG0180004294.01	MsG0180004767.01	0.803795	2.940133e-49	1.491100e-46
MsG0180004294.01	MsG0180005049.01	0.800020	1.752690e-48	8.074880e-46
MsG0180004294.01	MsG0180005407.01	0.883273	5.158522e-71	3.248247e-67
MsG0180004294.01	MsG0180005443.01	0.835317	1.800141e-56	2.193615e-53
MsG0180004294.01	MsG0180005445.01	0.845274	4.498791e-59	7.457970e-56
MsG0180004294.01	MsG0180005451.01	0.817688	2.896391e-52	2.123799e-49
MsG0180004294.01	MsG0180005765.01	0.830472	2.881591e-55	3.038759e-52
MsG0180004294.01	MsG0180005786.01	0.827662	1.382596e-54	1.343419e-51
MsG0180004294.01	MsG0180005842.01	0.851837	6.840147e-61	1.402197e-57
MsG0180004294.01	MsG0180005878.01	0.822193	2.700346e-53	2.244305e-50
MsG0180004294.01	MsG0180005944.01	0.802170	6.371074e-49	3.099604e-46
MsG0180004294.01	MsG0180005993.01	0.808328	3.269623e-50	1.864204e-47
MsG0180004294.01	MsG0180006098.01	0.806146	9.477362e-50	5.105928e-47
MsG0180004294.01	MsG0180006155.01	0.875872	2.187463e-68	1.038273e-64
MsG0180004294.01	MsG0280006313.01	0.833078	6.557554e-56	7.471614e-53
MsG0180004294.01	MsG0280006365.01	0.845630	3.601976e-59	6.039741e-56
MsG0180004294.01	MsG0280006461.01	0.809461	1.870599e-50	1.098948e-47
MsG0180004294.01	MsG0280006678.01	0.804310	2.297354e-49	1.180612e-46
MsG0180004294.01	MsG0280007088.01	0.837774	4.261028e-57	5.593320e-54
MsG0180004294.01	MsG0280007109.01	0.824316	8.621585e-54	7.609534e-51
MsG0180004294.01	MsG0280007153.01	0.823923	1.066580e-53	9.310556e-51
MsG0180004294.01	MsG0280007161.01	0.801725	7.862634e-49	3.782135e-46
MsG0180004294.01	MsG0280007321.01	0.822878	1.871548e-53	1.586219e-50
MsG0180004294.01	MsG0280007564.01	0.837548	4.871799e-57	6.349868e-54
MsG0180004294.01	MsG0280007585.01	0.870579	1.311396e-66	5.126877e-63
MsG0180004294.01	MsG0280007645.01	0.801263	9.780395e-49	4.650164e-46
MsG0180004294.01	MsG0280007710.01	0.861026	1.369980e-63	3.821316e-60
MsG0180004294.01	MsG0280007745.01	0.813590	2.369985e-51	1.554126e-48
MsG0180004294.01	MsG0280007815.01	0.814545	1.459113e-51	9.818878e-49
MsG0180004294.01	MsG0280008502.01	0.854419	1.246241e-61	2.781403e-58
MsG0180004294.01	MsG0280008556.01	0.848133	7.442281e-60	1.352195e-56
MsG0180004294.01	MsG0280008970.01	0.807656	4.543183e-50	2.544810e-47
MsG0180004294.01	MsG0280009578.01	0.811360	7.278065e-51	4.495762e-48
MsG0180004294.01	MsG0280009748.01	0.802643	5.089162e-49	2.505939e-46
MsG0180004294.01	MsG0280010158.01	0.801340	9.431404e-49	4.492969e-46
MsG0180004294.01	MsG0280010233.01	0.876869	9.902439e-69	4.878296e-65
MsG0180004294.01	MsG0280010447.01	0.851907	6.537478e-61	1.342986e-57
MsG0180004294.01	MsG0280010560.01	0.867213	1.615646e-65	5.595423e-62
MsG0180004294.01	MsG0280010599.01	0.813399	2.611151e-51	1.703414e-48
MsG0180004294.01	MsG0280010976.01	0.802191	6.306772e-49	3.069906e-46
MsG0180004294.01	MsG0280011043.01	0.818901	1.538544e-52	1.166578e-49
MsG0180004294.01	MsG0280011141.01	0.818632	1.771387e-52	1.333027e-49
MsG0180004294.01	MsG0280011220.01	0.807572	4.734550e-50	2.646160e-47
MsG0180004294.01	MsG0280011293.01	0.811221	7.803739e-51	4.803000e-48
MsG0180004294.01	MsG0280011334.01	0.807128	5.881461e-50	3.249399e-47
MsG0180004294.01	MsG0380011729.01	0.832733	7.989447e-56	9.008807e-53
MsG0180004294.01	MsG0380012020.01	0.829730	4.371525e-55	4.512938e-52
MsG0180004294.01	MsG0380012460.01	0.816773	4.652584e-52	3.327090e-49
MsG0180004294.01	MsG0380013176.01	0.801302	9.601715e-49	4.569770e-46
MsG0180004294.01	MsG0380013754.01	0.842126	3.127433e-58	4.695608e-55
MsG0180004294.01	MsG0380014128.01	0.815605	8.485087e-52	5.877332e-49
MsG0180004294.01	MsG0380014248.01	0.802288	6.023458e-49	2.939475e-46
MsG0180004294.01	MsG0380014365.01	0.816078	6.657525e-52	4.671321e-49
MsG0180004294.01	MsG0380014489.01	0.802316	5.944846e-49	2.903242e-46
MsG0180004294.01	MsG0380014537.01	0.842780	2.098559e-58	3.215779e-55
MsG0180004294.01	MsG0380014541.01	0.822117	2.811933e-53	2.332063e-50
MsG0180004294.01	MsG0380014885.01	0.803546	3.310232e-49	1.668183e-46
MsG0180004294.01	MsG0380014908.01	0.801148	1.032194e-48	4.893640e-46
MsG0180004294.01	MsG0380015512.01	0.828783	7.417808e-55	7.447007e-52
MsG0180004294.01	MsG0380015555.01	0.807616	4.633609e-50	2.592721e-47
MsG0180004294.01	MsG0380015564.01	0.809348	1.978361e-50	1.158812e-47
MsG0180004294.01	MsG0380015713.01	0.811484	6.841606e-51	4.240234e-48
MsG0180004294.01	MsG0380016513.01	0.806800	6.899366e-50	3.779683e-47
MsG0180004294.01	MsG0380016561.01	0.811369	7.245198e-51	4.476615e-48
MsG0180004294.01	MsG0380016923.01	0.807589	4.695546e-50	2.625444e-47
MsG0180004294.01	MsG0380016971.01	0.822285	2.571257e-53	2.142861e-50
MsG0180004294.01	MsG0380017140.01	0.805312	1.418388e-49	7.479833e-47
MsG0180004294.01	MsG0380017210.01	0.808538	2.948626e-50	1.690355e-47
MsG0180004294.01	MsG0380017375.01	0.805870	1.083203e-49	5.794924e-47
MsG0180004294.01	MsG0380017403.01	0.819464	1.145352e-52	8.820691e-50
MsG0180004294.01	MsG0380017719.01	0.809985	1.443493e-50	8.598932e-48
MsG0180004294.01	MsG0380017864.01	0.813076	3.073493e-51	1.987359e-48
MsG0180004294.01	MsG0480018462.01	0.824294	8.728037e-54	7.698463e-51
MsG0180004294.01	MsG0480018479.01	0.810135	1.340069e-50	8.013626e-48
MsG0180004294.01	MsG0480019404.01	0.827187	1.796984e-54	1.722224e-51
MsG0180004294.01	MsG0480019772.01	0.811589	6.492910e-51	4.034988e-48
MsG0180004294.01	MsG0480019785.01	0.808195	3.489521e-50	1.982590e-47
MsG0180004294.01	MsG0480019970.01	0.802167	6.378681e-49	3.103136e-46
MsG0180004294.01	MsG0480020331.01	0.824941	6.143691e-54	5.521503e-51
MsG0180004294.01	MsG0480020447.01	0.833936	4.003515e-56	4.680489e-53
MsG0180004294.01	MsG0480020916.01	0.835987	1.218184e-56	1.514443e-53
MsG0180004294.01	MsG0480021409.01	0.821991	3.007432e-53	2.485240e-50
MsG0180004294.01	MsG0480021432.01	0.812912	3.338319e-51	2.149189e-48
MsG0180004294.01	MsG0480021465.01	0.823214	1.562335e-53	1.336731e-50
MsG0180004294.01	MsG0480021772.01	0.804769	1.842235e-49	9.580555e-47
MsG0180004294.01	MsG0480021953.01	0.818608	1.793670e-52	1.348882e-49
MsG0180004294.01	MsG0480021998.01	0.804981	1.663543e-49	8.698497e-47
MsG0180004294.01	MsG0480022292.01	0.847634	1.021344e-59	1.826757e-56
MsG0180004294.01	MsG0480022467.01	0.868061	8.633443e-66	3.081215e-62
MsG0180004294.01	MsG0480022476.01	0.813289	2.760039e-51	1.795088e-48
MsG0180004294.01	MsG0480022678.01	0.814411	1.561783e-51	1.047250e-48
MsG0180004294.01	MsG0480022696.01	0.823030	1.724566e-53	1.468000e-50
MsG0180004294.01	MsG0480022711.01	0.802711	4.927298e-49	2.430544e-46
MsG0180004294.01	MsG0480022717.01	0.814975	1.171236e-51	7.973613e-49
MsG0180004294.01	MsG0480022985.01	0.811246	7.707890e-51	4.747037e-48
MsG0180004294.01	MsG0480023014.01	0.843985	1.000155e-58	1.591972e-55
MsG0180004294.01	MsG0480023025.01	0.836181	1.087600e-56	1.360288e-53
MsG0180004294.01	MsG0480023055.01	0.818573	1.826683e-52	1.372410e-49
MsG0180004294.01	MsG0480023224.01	0.829571	4.778130e-55	4.909125e-52
MsG0180004294.01	MsG0480023245.01	0.803497	3.388780e-49	1.705578e-46
MsG0180004294.01	MsG0480023412.01	0.833904	4.079279e-56	4.764451e-53
MsG0180004294.01	MsG0480023415.01	0.807005	6.243062e-50	3.438355e-47
MsG0180004294.01	MsG0480023600.01	0.847664	1.001970e-59	1.793966e-56
MsG0180004294.01	MsG0480023873.01	0.847845	8.938415e-60	1.609481e-56
MsG0180004294.01	MsG0580024197.01	0.804366	2.235736e-49	1.150636e-46
MsG0180004294.01	MsG0580024251.01	0.805567	1.254450e-49	6.658517e-47
MsG0180004294.01	MsG0580024353.01	0.836466	9.206307e-57	1.161203e-53
MsG0180004294.01	MsG0580024536.01	0.867027	1.851209e-65	6.369295e-62
MsG0180004294.01	MsG0580024781.01	0.816768	4.664706e-52	3.335281e-49
MsG0180004294.01	MsG0580024960.01	0.816864	4.439636e-52	3.182764e-49
MsG0180004294.01	MsG0580025026.01	0.805353	1.391073e-49	7.343345e-47
MsG0180004294.01	MsG0580025439.01	0.818504	1.894044e-52	1.420346e-49
MsG0180004294.01	MsG0580025986.01	0.849894	2.412283e-60	4.641663e-57
MsG0180004294.01	MsG0580026225.01	0.843152	1.669906e-58	2.588989e-55
MsG0180004294.01	MsG0580027891.01	0.805342	1.397844e-49	7.377131e-47
MsG0180004294.01	MsG0580029349.01	0.823607	1.264499e-53	1.094004e-50
MsG0180004294.01	MsG0580029629.01	0.811529	6.688149e-51	4.150104e-48
MsG0180004294.01	MsG0580029651.01	0.806275	8.904559e-50	4.813282e-47
MsG0180004294.01	MsG0580029658.01	0.818736	1.677211e-52	1.265826e-49
MsG0180004294.01	MsG0580029762.01	0.843082	1.743364e-58	2.696836e-55
MsG0180004294.01	MsG0580029926.01	0.808319	3.283691e-50	1.871752e-47
MsG0180004294.01	MsG0580030003.01	0.834604	2.722459e-56	3.246988e-53
MsG0180004294.01	MsG0580030235.01	0.802340	5.877771e-49	2.872123e-46
MsG0180004294.01	MsG0680030413.01	0.861412	1.044575e-63	2.953438e-60
MsG0180004294.01	MsG0680031301.01	0.824223	9.067660e-54	7.982591e-51
MsG0180004294.01	MsG0680031651.01	0.824350	8.464309e-54	7.478080e-51
MsG0180004294.01	MsG0680031699.01	0.809509	1.826861e-50	1.074622e-47
MsG0180004294.01	MsG0680032075.01	0.819915	9.037095e-53	7.047864e-50
MsG0180004294.01	MsG0680034037.01	0.840830	6.863628e-58	9.896987e-55
MsG0180004294.01	MsG0680034757.01	0.804330	2.275255e-49	1.169858e-46
MsG0180004294.01	MsG0680035306.01	0.813421	2.581426e-51	1.685031e-48
MsG0180004294.01	MsG0680035340.01	0.801660	8.109767e-49	3.894578e-46
MsG0180004294.01	MsG0780036430.01	0.816602	5.082168e-52	3.617310e-49
MsG0180004294.01	MsG0780036663.01	0.820262	7.527819e-53	5.927382e-50
MsG0180004294.01	MsG0780036773.01	0.804973	1.669963e-49	8.730490e-47
MsG0180004294.01	MsG0780037141.01	0.828647	8.003321e-55	8.003468e-52
MsG0180004294.01	MsG0780038565.01	0.823509	1.333040e-53	1.150117e-50
MsG0180004294.01	MsG0780039713.01	0.817762	2.787699e-52	2.048286e-49
MsG0180004294.01	MsG0780039777.01	0.801763	7.723479e-49	3.718734e-46
MsG0180004294.01	MsG0780040044.01	0.804465	2.132776e-49	1.100399e-46
MsG0180004294.01	MsG0780040254.01	0.806643	7.447007e-50	4.062996e-47
MsG0180004294.01	MsG0780040262.01	0.811949	5.420593e-51	3.401147e-48
MsG0180004294.01	MsG0780040597.01	0.804181	2.443301e-49	1.251467e-46
MsG0180004294.01	MsG0780040712.01	0.810766	9.790306e-51	5.953514e-48
MsG0180004294.01	MsG0780040845.01	0.804477	2.119919e-49	1.094115e-46
MsG0180004294.01	MsG0780041065.01	0.832944	7.078192e-56	8.031790e-53
MsG0180004294.01	MsG0780041127.01	0.818481	1.916560e-52	1.436369e-49
MsG0180004294.01	MsG0780041368.01	0.827080	1.906294e-54	1.821261e-51
MsG0180004294.01	MsG0780041399.01	0.805797	1.122176e-49	5.992118e-47
MsG0180004294.01	MsG0780041498.01	0.876101	1.824350e-68	8.734722e-65
MsG0180004294.01	MsG0780041754.01	0.827813	1.271679e-54	1.241051e-51
MsG0180004294.01	MsG0780041796.01	0.821695	3.521304e-53	2.885645e-50
MsG0180004294.01	MsG0880042052.01	0.875304	3.424665e-68	1.591190e-64
MsG0180004294.01	MsG0880042324.01	0.813141	2.973651e-51	1.926187e-48
MsG0180004294.01	MsG0880042518.01	0.814804	1.278288e-51	8.662408e-49
MsG0180004294.01	MsG0880042519.01	0.801292	9.646626e-49	4.590013e-46
MsG0180004294.01	MsG0880042792.01	0.829200	5.881633e-55	5.978417e-52
MsG0180004294.01	MsG0880042829.01	0.804113	2.524238e-49	1.290712e-46
MsG0180004294.01	MsG0880042848.01	0.812649	3.812089e-51	2.437264e-48
MsG0180004294.01	MsG0880042972.01	0.803704	3.070399e-49	1.553473e-46
MsG0180004294.01	MsG0880043116.01	0.819082	1.400088e-52	1.066806e-49
MsG0180004294.01	MsG0880043163.01	0.822151	2.761338e-53	2.292311e-50
MsG0180004294.01	MsG0880043532.01	0.843261	1.562127e-58	2.430493e-55
MsG0180004294.01	MsG0880043804.01	0.835349	1.767586e-56	2.155936e-53
MsG0180004294.01	MsG0880045393.01	0.831886	1.294367e-55	1.423628e-52
MsG0180004294.01	MsG0880045619.01	0.825008	5.924753e-54	5.335053e-51
MsG0180004294.01	MsG0880045736.01	0.806883	6.625792e-50	3.637626e-47
MsG0180004294.01	MsG0880045748.01	0.802225	6.205960e-49	3.023502e-46
MsG0180004294.01	MsG0880045754.01	0.898992	3.078285e-77	3.730196e-73
MsG0180004294.01	MsG0880046027.01	0.837572	4.803446e-57	6.265414e-54
MsG0180004294.01	MsG0880046450.01	0.826254	3.001421e-54	2.800049e-51
MsG0180004294.01	MsG0880046532.01	0.824933	6.168975e-54	5.542992e-51
MsG0180004294.01	MsG0880047470.01	0.802251	6.130741e-49	2.988893e-46
MsG0180004294.01	MsG0880047472.01	0.810713	1.005222e-50	6.104160e-48
MsG0180004294.01	MsG0880047473.01	0.808563	2.913024e-50	1.671010e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004294.01.T01	MTR_8g077990	85.202	223	22	3	1	213	25	246	8.24e-95	291
MsG0180004294.01.T01	MTR_8g077990	52.000	150	50	3	13	141	172	320	5.07e-15	73.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 25 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTATGTGTGCCTTCTTCACT+TGG	0.367399	1:-76195398	None:intergenic
CAGATCAGCTACATTGCTTT+CGG	0.400572	1:-76194928	None:intergenic
AGAAGCAATGAAGGTGATAA+TGG	0.417384	1:+76195155	MsG0180004294.01.T01:CDS
TAGAAGCAATGGAGGTGAAG+AGG	0.481860	1:+76195529	MsG0180004294.01.T01:CDS
AAGAAGAAACCAAGTGAAGA+AGG	0.493411	1:+76195389	MsG0180004294.01.T01:CDS
GAGTGAGGAAAGCAAACATA+AGG	0.496559	1:+76195214	MsG0180004294.01.T01:CDS
TGAAGGTGATAATGGCGAGC+AGG	0.499793	1:+76195163	MsG0180004294.01.T01:CDS
TGTAATGACTTTAGAAGCAA+TGG	0.511180	1:+76195518	MsG0180004294.01.T01:CDS
AACAACTTTAGAAGCAATGA+AGG	0.512264	1:+76195146	MsG0180004294.01.T01:CDS
AAGTGAAGAAGGCACACATA+AGG	0.523008	1:+76195400	MsG0180004294.01.T01:CDS
AAGATGGTGAGCGAGAGAAA+AGG	0.524139	1:+76195357	MsG0180004294.01.T01:CDS
AAAAGCAATAAAAGTGAAGA+TGG	0.528970	1:+76194960	MsG0180004294.01.T01:CDS
AAGAAGCGGAAGCTAGTTGA+AGG	0.534153	1:+76195104	MsG0180004294.01.T01:CDS
AAAAGTGAAGATGGCGAGCA+AGG	0.538269	1:+76194969	MsG0180004294.01.T01:CDS
AGAAGCAATGGAGGTGAAGA+GGG	0.572295	1:+76195530	MsG0180004294.01.T01:CDS
GAAGGTGATAATGGCGAGCA+GGG	0.580967	1:+76195164	MsG0180004294.01.T01:CDS
AGAAGCAATGATGATGAAGA+TGG	0.598793	1:+76195341	MsG0180004294.01.T01:CDS
TGAAGGAAACACACTTGAGG+AGG	0.607482	1:+76195121	MsG0180004294.01.T01:CDS
AATTGAAGGAAGCACACTGA+AGG	0.616212	1:+76195304	MsG0180004294.01.T01:CDS
AAATGTGAAGCTGACGACCA+AGG	0.655741	1:+76195260	MsG0180004294.01.T01:CDS
AATGACTTTAGAAGCAATGG+AGG	0.658645	1:+76195521	MsG0180004294.01.T01:CDS
GAAGGAAACACACTTGAGGA+GGG	0.660891	1:+76195122	MsG0180004294.01.T01:CDS
AATGATTGTGAAGATGACCA+AGG	0.698674	1:+76195443	MsG0180004294.01.T01:CDS
AGTTGAAGGAAACACACTTG+AGG	0.699110	1:+76195118	MsG0180004294.01.T01:CDS
GAATGTGAAGATGATGACCA+CGG	0.736913	1:+76195068	MsG0180004294.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAGCAGAAATTAATTGA+AGG	+	Chr1:76195479-76195498	MsG0180004294.01.T01:CDS	20.0%
!	AAAAGCAATAAAAGTGAAGA+TGG	+	Chr1:76194960-76194979	MsG0180004294.01.T01:CDS	25.0%
!	ATGAAAAAGAAGCTAATTGA+AGG	+	Chr1:76195290-76195309	MsG0180004294.01.T01:CDS	25.0%
!!!	ATCTTCACTTTTATTGCTTT+TGG	-	Chr1:76194961-76194980	None:intergenic	25.0%
!!!	TTTTTTTCGTCTTTTTTCCT+TGG	-	Chr1:76195463-76195482	None:intergenic	25.0%
	GAAAAAGGAAAAATTGAGTG+AGG	+	Chr1:76195199-76195218	MsG0180004294.01.T01:CDS	30.0%
	GAAAGAAGATGAAAAAGAAG+CGG	+	Chr1:76195090-76195109	MsG0180004294.01.T01:CDS	30.0%
	GGGAAAGAAGAAAAAGAAAA+AGG	+	Chr1:76195184-76195203	MsG0180004294.01.T01:CDS	30.0%
!	AACAACTTTAGAAGCAATGA+AGG	+	Chr1:76195146-76195165	MsG0180004294.01.T01:CDS	30.0%
!	TGTAATGACTTTAGAAGCAA+TGG	+	Chr1:76195518-76195537	MsG0180004294.01.T01:CDS	30.0%
!!	CTTTTATTGCTTTTGGTCAA+AGG	-	Chr1:76194954-76194973	None:intergenic	30.0%
!!	TCTTTTTCATCTTCTTTCCT+TGG	-	Chr1:76195280-76195299	None:intergenic	30.0%
	AAGAAGAAACCAAGTGAAGA+AGG	+	Chr1:76195389-76195408	MsG0180004294.01.T01:CDS	35.0%
	AATGATTGTGAAGATGACCA+AGG	+	Chr1:76195443-76195462	MsG0180004294.01.T01:CDS	35.0%
	AGAAGCAATGAAGGTGATAA+TGG	+	Chr1:76195155-76195174	MsG0180004294.01.T01:CDS	35.0%
!	AATGACTTTAGAAGCAATGG+AGG	+	Chr1:76195521-76195540	MsG0180004294.01.T01:CDS	35.0%
!	AGAAGCAATGATGATGAAGA+TGG	+	Chr1:76195341-76195360	MsG0180004294.01.T01:CDS	35.0%
!!	TCTTTTTCATCTTCTTTCCG+TGG	-	Chr1:76195088-76195107	None:intergenic	35.0%
	AGTTGAAGGAAACACACTTG+AGG	+	Chr1:76195118-76195137	MsG0180004294.01.T01:CDS	40.0%
	CAGATCAGCTACATTGCTTT+CGG	-	Chr1:76194931-76194950	None:intergenic	40.0%
	GAATGTGAAGATGATGACCA+CGG	+	Chr1:76195068-76195087	MsG0180004294.01.T01:CDS	40.0%
	GAGTGAGGAAAGCAAACATA+AGG	+	Chr1:76195214-76195233	MsG0180004294.01.T01:CDS	40.0%
	TTATGTGTGCCTTCTTCACT+TGG	-	Chr1:76195401-76195420	None:intergenic	40.0%
!	AAGTGAAGAAGGCACACATA+AGG	+	Chr1:76195400-76195419	MsG0180004294.01.T01:CDS	40.0%
!	AATTGAAGGAAGCACACTGA+AGG	+	Chr1:76195304-76195323	MsG0180004294.01.T01:CDS	40.0%
	AAAAGTGAAGATGGCGAGCA+AGG	+	Chr1:76194969-76194988	MsG0180004294.01.T01:CDS	45.0%
	AAATGTGAAGCTGACGACCA+AGG	+	Chr1:76195260-76195279	MsG0180004294.01.T01:CDS	45.0%
	AAGAAGCGGAAGCTAGTTGA+AGG	+	Chr1:76195104-76195123	MsG0180004294.01.T01:CDS	45.0%
	AAGATGGTGAGCGAGAGAAA+AGG	+	Chr1:76195357-76195376	MsG0180004294.01.T01:CDS	45.0%
	AGAAGCAATGGAGGTGAAGA+GGG	+	Chr1:76195530-76195549	MsG0180004294.01.T01:CDS	45.0%
	GAAGGAAACACACTTGAGGA+GGG	+	Chr1:76195122-76195141	MsG0180004294.01.T01:CDS	45.0%
	TAGAAGCAATGGAGGTGAAG+AGG	+	Chr1:76195529-76195548	MsG0180004294.01.T01:CDS	45.0%
	TGAAGGAAACACACTTGAGG+AGG	+	Chr1:76195121-76195140	MsG0180004294.01.T01:CDS	45.0%
!!	GAAGGTGATAATGGCGAGCA+GGG	+	Chr1:76195164-76195183	MsG0180004294.01.T01:CDS	50.0%
!!	TGAAGGTGATAATGGCGAGC+AGG	+	Chr1:76195163-76195182	MsG0180004294.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	76194924	76195574	76194924	ID=MsG0180004294.01;Name=MsG0180004294.01
Chr1	mRNA	76194924	76195574	76194924	ID=MsG0180004294.01.T01;Parent=MsG0180004294.01;Name=MsG0180004294.01.T01;_AED=0.42;_eAED=0.42;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|216
Chr1	exon	76194924	76195574	76194924	ID=MsG0180004294.01.T01:exon:12580;Parent=MsG0180004294.01.T01
Chr1	CDS	76194924	76195574	76194924	ID=MsG0180004294.01.T01:cds;Parent=MsG0180004294.01.T01

Gene Sequence

>MsG0180004294.01.T01

ATGTCCGAAAGCAATGTAGCTGATCTGCCTTTGACCAAAAGCAATAAAAGTGAAGATGGCGAGCAAGGCAAAAAGAAAAAGAAAAAGAAGAAGAAACTGAGTGAAGAAAGCAATCATAAGAAGTATAATGAGTTCAAAAGCAATGAATGTGAAGATGATGACCACGGAAAGAAGATGAAAAAGAAGCGGAAGCTAGTTGAAGGAAACACACTTGAGGAGGGTAACAACTTTAGAAGCAATGAAGGTGATAATGGCGAGCAGGGAAAGAAGAAAAAGAAAAAGGAAAAATTGAGTGAGGAAAGCAAACATAAGGAGTATAACGAGTTCAAAAGTAACAAATGTGAAGCTGACGACCAAGGAAAGAAGATGAAAAAGAAGCTAATTGAAGGAAGCACACTGAAGGAATGTAACGACTTTAGAAGCAATGATGATGAAGATGGTGAGCGAGAGAAAAGGATGAAGAAAAAGAAGAAACCAAGTGAAGAAGGCACACATAAGGACTATAGTGAGTTCAAAAGCAATGATTGTGAAGATGACCAAGGAAAAAAGACGAAAAAAAAGCAGAAATTAATTGAAGGAAACACACTTAATGAGTGTAATGACTTTAGAAGCAATGGAGGTGAAGAGGGTGAGCAAGACAAGAGAAGATGA

Protein sequence

>MsG0180004294.01.T01

MSESNVADLPLTKSNKSEDGEQGKKKKKKKKKLSEESNHKKYNEFKSNECEDDDHGKKMKKKRKLVEGNTLEEGNNFRSNEGDNGEQGKKKKKKEKLSEESKHKEYNEFKSNKCEADDQGKKMKKKLIEGSTLKECNDFRSNDDEDGEREKRMKKKKKPSEEGTHKDYSEFKSNDCEDDQGKKTKKKQKLIEGNTLNECNDFRSNGGEEGEQDKRR*