AlfalfaGEDB | MsG0180004294.01

M. sativa cultivar ZhongmuNo.1 / MsG0180004294.01

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004294.01.T01	RHN42138.1	85.202	223	22	3	1	213	25	246	2.17E-94	287
MsG0180004294.01.T01	RHN42138.1	70.408	98	27	2	13	109	172	268	3.70E-13	77.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004294.01.T01	A0A396GP03	85.202	223	22	3	1	213	25	246	1.04e-94	287
MsG0180004294.01.T01	G7LEV6	85.202	223	22	3	1	213	25	246	3.25e-91	291

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048058.01	MsG0180004294.01	0.814807	1.276646e-51	8.651877e-49
MsG0080048461.01	MsG0180004294.01	0.801207	1.003900e-48	4.766394e-46
MsG0080048964.01	MsG0180004294.01	0.829019	6.503736e-55	6.574464e-52
MsG0180000001.01	MsG0180004294.01	0.802439	5.606132e-49	2.746298e-46
MsG0180000101.01	MsG0180004294.01	0.803703	3.072247e-49	1.554366e-46
MsG0180000235.01	MsG0180004294.01	0.816007	6.903405e-52	4.834360e-49
MsG0180000703.01	MsG0180004294.01	0.834336	3.178661e-56	3.760846e-53
MsG0180001002.01	MsG0180004294.01	0.803311	3.703974e-49	1.855192e-46
MsG0180001294.01	MsG0180004294.01	0.832755	7.886565e-56	8.898702e-53
MsG0180001599.01	MsG0180004294.01	0.815601	8.501544e-52	5.888071e-49

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004294.01.T01	MTR_8g077990	85.202	223	22	3	1	213	25	246	8.24e-95	291
MsG0180004294.01.T01	MTR_8g077990	52.000	150	50	3	13	141	172	320	5.07e-15	73.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 25 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCAATAAAAGTGAAGA+TGG	0.528970	1:+76194960	MsG0180004294.01.T01:CDS
AAAAGTGAAGATGGCGAGCA+AGG	0.538269	1:+76194969	MsG0180004294.01.T01:CDS
AAATGTGAAGCTGACGACCA+AGG	0.655741	1:+76195260	MsG0180004294.01.T01:CDS
AACAACTTTAGAAGCAATGA+AGG	0.512264	1:+76195146	MsG0180004294.01.T01:CDS
AAGAAGAAACCAAGTGAAGA+AGG	0.493411	1:+76195389	MsG0180004294.01.T01:CDS
AAGAAGCGGAAGCTAGTTGA+AGG	0.534153	1:+76195104	MsG0180004294.01.T01:CDS
AAGATGGTGAGCGAGAGAAA+AGG	0.524139	1:+76195357	MsG0180004294.01.T01:CDS
AAGTGAAGAAGGCACACATA+AGG	0.523008	1:+76195400	MsG0180004294.01.T01:CDS
AATGACTTTAGAAGCAATGG+AGG	0.658645	1:+76195521	MsG0180004294.01.T01:CDS
AATGATTGTGAAGATGACCA+AGG	0.698674	1:+76195443	MsG0180004294.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAAAAAGGAAAAATTGAGTG+AGG	+	Chr1:76195199-76195218	MsG0180004294.01.T01:CDS	30.0%
GAAAGAAGATGAAAAAGAAG+CGG	+	Chr1:76195090-76195109	MsG0180004294.01.T01:CDS	30.0%
GGGAAAGAAGAAAAAGAAAA+AGG	+	Chr1:76195184-76195203	MsG0180004294.01.T01:CDS	30.0%
AAGAAGAAACCAAGTGAAGA+AGG	+	Chr1:76195389-76195408	MsG0180004294.01.T01:CDS	35.0%
AATGATTGTGAAGATGACCA+AGG	+	Chr1:76195443-76195462	MsG0180004294.01.T01:CDS	35.0%
AGAAGCAATGAAGGTGATAA+TGG	+	Chr1:76195155-76195174	MsG0180004294.01.T01:CDS	35.0%
AGTTGAAGGAAACACACTTG+AGG	+	Chr1:76195118-76195137	MsG0180004294.01.T01:CDS	40.0%
CAGATCAGCTACATTGCTTT+CGG	-	Chr1:76194931-76194950	None:intergenic	40.0%
GAATGTGAAGATGATGACCA+CGG	+	Chr1:76195068-76195087	MsG0180004294.01.T01:CDS	40.0%
GAGTGAGGAAAGCAAACATA+AGG	+	Chr1:76195214-76195233	MsG0180004294.01.T01:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	76194924	76195574	76194924	ID=MsG0180004294.01;Name=MsG0180004294.01
Chr1	mRNA	76194924	76195574	76194924	ID=MsG0180004294.01.T01;Parent=MsG0180004294.01;Name=MsG0180004294.01.T01;_AED=0.42;_eAED=0.42;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|216
Chr1	exon	76194924	76195574	76194924	ID=MsG0180004294.01.T01:exon:12580;Parent=MsG0180004294.01.T01
Chr1	CDS	76194924	76195574	76194924	ID=MsG0180004294.01.T01:cds;Parent=MsG0180004294.01.T01

Gene Sequence

>MsG0180004294.01.T01

ATGTCCGAAAGCAATGTAGCTGATCTGCCTTTGACCAAAAGCAATAAAAGTGAAGATGGCGAGCAAGGCAAAAAGAAAAAGAAAAAGAAGAAGAAACTGAGTGAAGAAAGCAATCATAAGAAGTATAATGAGTTCAAAAGCAATGAATGTGAAGATGATGACCACGGAAAGAAGATGAAAAAGAAGCGGAAGCTAGTTGAAGGAAACACACTTGAGGAGGGTAACAACTTTAGAAGCAATGAAGGTGATAATGGCGAGCAGGGAAAGAAGAAAAAGAAAAAGGAAAAATTGAGTGAGGAAAGCAAACATAAGGAGTATAACGAGTTCAAAAGTAACAAATGTGAAGCTGACGACCAAGGAAAGAAGATGAAAAAGAAGCTAATTGAAGGAAGCACACTGAAGGAATGTAACGACTTTAGAAGCAATGATGATGAAGATGGTGAGCGAGAGAAAAGGATGAAGAAAAAGAAGAAACCAAGTGAAGAAGGCACACATAAGGACTATAGTGAGTTCAAAAGCAATGATTGTGAAGATGACCAAGGAAAAAAGACGAAAAAAAAGCAGAAATTAATTGAAGGAAACACACTTAATGAGTGTAATGACTTTAGAAGCAATGGAGGTGAAGAGGGTGAGCAAGACAAGAGAAGATGA

Protein sequence

>MsG0180004294.01.T01

MSESNVADLPLTKSNKSEDGEQGKKKKKKKKKLSEESNHKKYNEFKSNECEDDDHGKKMKKKRKLVEGNTLEEGNNFRSNEGDNGEQGKKKKKKEKLSEESKHKEYNEFKSNKCEADDQGKKMKKKLIEGSTLKECNDFRSNDDEDGEREKRMKKKKKPSEEGTHKDYSEFKSNDCEDDQGKKTKKKQKLIEGNTLNECNDFRSNGGEEGEQDKRR*