AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022476.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022476.01.T01 MTR_4g090460 95.604 273 12 0 2 274 112 384 0.0 510
MsG0480022476.01.T01 MTR_4g090460 83.333 90 15 0 89 178 108 197 3.43e-43 155
MsG0480022476.01.T01 MTR_4g090460 95.413 218 10 0 57 274 1 218 1.16e-140 402
MsG0480022476.01.T01 MTR_4g090460 84.884 86 13 0 2 87 37 122 6.92e-44 154
MsG0480022476.01.T01 MTR_2g012600 56.180 267 110 5 15 274 303 569 2.53e-68 225
MsG0480022476.01.T01 MTR_2g012600 56.548 168 66 3 5 168 109 273 1.02e-42 155
MsG0480022476.01.T01 MTR_2g012600 55.952 168 67 3 5 168 201 365 2.70e-42 154
MsG0480022476.01.T01 MTR_2g012600 64.384 73 26 0 11 83 391 463 5.47e-19 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 53 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGATCTCGCCTGGTGTTTC+AGG 0.123019 4:+73066264 MsG0480022476.01.T01:CDS
GAGATTACGCCTGGTGTTTC+AGG 0.146830 4:+73066537 MsG0480022476.01.T01:CDS
ACATGTTCCTTCACAGAAAT+TGG 0.179613 4:+73066389 MsG0480022476.01.T01:CDS
ACTCGCTTCATTAATATTTC+TGG 0.224605 4:-73066673 None:intergenic
TGTGAAGGAACATGTGATTC+TGG 0.321301 4:-73066381 None:intergenic
TGTGAAGGAACATCTAATTC+TGG 0.326164 4:-73066108 None:intergenic
GGTGAGGTTGTCAAATCTTC+TGG 0.339149 4:+73066759 MsG0480022476.01.T01:CDS
GGAGAACACGAACTTGAAAA+AGG 0.362922 4:+73066791 MsG0480022476.01.T01:CDS
CAGAAGACATTGCTGGAACT+TGG 0.372442 4:-73066506 None:intergenic
GACAACCTCACCACAGTTGC+TGG 0.380777 4:-73066748 None:intergenic
ATTCAAAACTTTCTCATGTT+CGG 0.403224 4:-73066565 None:intergenic
TCAAAGGATGGGCTATCTGC+TGG 0.419315 4:+73066168 MsG0480022476.01.T01:CDS
CACATCAAACCGATACAAAC+TGG 0.424104 4:+73066199 MsG0480022476.01.T01:CDS
AAAGGAGAGGAAAGAGAAAG+AGG 0.432152 4:+73066640 MsG0480022476.01.T01:CDS
ATGTCTTCAGAAGACATTGC+TGG 0.442846 4:-73066240 None:intergenic
ATGTCTTCAGAAGACATTGC+TGG 0.442846 4:-73066513 None:intergenic
CAGAAGACATTGCTGGAACC+TGG 0.448111 4:-73066233 None:intergenic
AGATCTCGCCTGGTGTTTCA+GGG 0.481234 4:+73066265 MsG0480022476.01.T01:CDS
TGGTAGAACTTTCTTTCAGC+CGG 0.484226 4:-73066728 None:intergenic
ATCAACTAAAGGGAGTGAAC+AGG 0.485140 4:-73066915 None:intergenic
ACTAAAGAGTTGTCAAAGGA+TGG 0.489392 4:+73066156 MsG0480022476.01.T01:CDS
AGATTACGCCTGGTGTTTCA+GGG 0.501278 4:+73066538 MsG0480022476.01.T01:CDS
CTGTCGCAAGCATGATAGCC+AGG 0.522128 4:+73066878 MsG0480022476.01.T01:CDS
GAAGACATGGAGATCTCGCC+TGG 0.524803 4:+73066255 MsG0480022476.01.T01:CDS
ACTAAAGAGACGTCAAAGGA+TGG 0.530356 4:+73066429 MsG0480022476.01.T01:CDS
GATAAGTTGACAGATACAGC+TGG 0.532080 4:+73066318 MsG0480022476.01.T01:CDS
GAGAGGAAAGAGAAAGAGGA+AGG 0.532691 4:+73066644 MsG0480022476.01.T01:CDS
GGTAGAACTTTCTTTCAGCC+GGG 0.535651 4:-73066727 None:intergenic
TCAAAGGATGGGTTATCTGC+TGG 0.536634 4:+73066441 MsG0480022476.01.T01:CDS
ACTCTCTCCAATTTCTGTGA+AGG 0.536812 4:-73066396 None:intergenic
AAACTGGTCTCATGAATGCC+AGG 0.546467 4:+73066215 MsG0480022476.01.T01:CDS
CTTGTTGACAACAACTCAGT+TGG 0.548851 4:+73066084 MsG0480022476.01.T01:CDS
CAAAGGATGGGCTATCTGCT+GGG 0.550496 4:+73066169 MsG0480022476.01.T01:CDS
TTCATGAGACCAGTTTGTAT+CGG 0.551753 4:-73066208 None:intergenic
GAAGACATGGAGATTACGCC+TGG 0.563944 4:+73066528 MsG0480022476.01.T01:CDS
CTAAAGAGTTGTCAAAGGAT+GGG 0.572945 4:+73066157 MsG0480022476.01.T01:CDS
ACTCTCTCTAATTTCTGTGA+AGG 0.579129 4:-73066123 None:intergenic
GGATGGAACGAAAGATCTGA+TGG 0.581786 4:+73066812 MsG0480022476.01.T01:CDS
CTAAAGAGACGTCAAAGGAT+GGG 0.581958 4:+73066430 MsG0480022476.01.T01:CDS
AGCAATGTCTTCTGAAGACA+TGG 0.586266 4:+73066242 MsG0480022476.01.T01:CDS
AGCAATGTCTTCTGAAGACA+TGG 0.586266 4:+73066515 MsG0480022476.01.T01:CDS
TTCTACTAAAGAGTTGTCAA+AGG 0.593159 4:+73066152 MsG0480022476.01.T01:CDS
TTGACAGAGACTGTATATGA+AGG 0.607121 4:+73066597 MsG0480022476.01.T01:CDS
GAAAGTTCTACCAGCAACTG+TGG 0.627439 4:+73066738 MsG0480022476.01.T01:CDS
TCAATAGACCCTGAAACACC+AGG 0.632152 4:-73066273 None:intergenic
TTCAACTAAAGAGACGTCAA+AGG 0.633541 4:+73066425 MsG0480022476.01.T01:CDS
TCGGTTGACCCTGAAACACC+AGG 0.635035 4:-73066546 None:intergenic
TTCTACCAGCAACTGTGGTG+AGG 0.636519 4:+73066743 MsG0480022476.01.T01:CDS
CAAATCTTCTGGTGTAACTG+AGG 0.640200 4:+73066770 MsG0480022476.01.T01:CDS
ATAAGTTGACAGATACAGCT+GGG 0.644236 4:+73066319 MsG0480022476.01.T01:CDS
GAGACTGTATATGAAGGACA+TGG 0.658527 4:+73066603 MsG0480022476.01.T01:CDS
CTGTATATGAAGGACATGGA+GGG 0.672772 4:+73066607 MsG0480022476.01.T01:CDS
ACTGTATATGAAGGACATGG+AGG 0.676971 4:+73066606 MsG0480022476.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATTCAAAACTTTCTCATGTT+CGG - Chr4:73066295-73066314 None:intergenic 25.0%
!!! GATTCTGTTTTGGAAATTAA+AGG + Chr4:73066843-73066862 MsG0480022476.01.T01:CDS 25.0%
ACTCGCTTCATTAATATTTC+TGG - Chr4:73066676-73066695 None:intergenic 30.0%
TTCTACTAAAGAGTTGTCAA+AGG + Chr4:73066152-73066171 MsG0480022476.01.T01:CDS 30.0%
!! ATTTTTCAATTGATGTGTCC+CGG + Chr4:73066709-73066728 MsG0480022476.01.T01:CDS 30.0%
!!! ATGGAGGGTGTTTTAAAAAA+AGG + Chr4:73066622-73066641 MsG0480022476.01.T01:CDS 30.0%
AACACGAACTTGAAAAAGGA+TGG + Chr4:73066795-73066814 MsG0480022476.01.T01:CDS 35.0%
ACATGTTCCTTCACAGAAAT+TGG + Chr4:73066389-73066408 MsG0480022476.01.T01:CDS 35.0%
ACTAAAGAGTTGTCAAAGGA+TGG + Chr4:73066156-73066175 MsG0480022476.01.T01:CDS 35.0%
ACTCTCTCTAATTTCTGTGA+AGG - Chr4:73066126-73066145 None:intergenic 35.0%
CTAAAGAGTTGTCAAAGGAT+GGG + Chr4:73066157-73066176 MsG0480022476.01.T01:CDS 35.0%
TGTGAAGGAACATCTAATTC+TGG - Chr4:73066111-73066130 None:intergenic 35.0%
TTCAACTAAAGAGACGTCAA+AGG + Chr4:73066425-73066444 MsG0480022476.01.T01:CDS 35.0%
TTCATGAGACCAGTTTGTAT+CGG - Chr4:73066211-73066230 None:intergenic 35.0%
TTGACAGAGACTGTATATGA+AGG + Chr4:73066597-73066616 MsG0480022476.01.T01:CDS 35.0%
! ATAAGTTGACAGATACAGCT+GGG + Chr4:73066319-73066338 MsG0480022476.01.T01:CDS 35.0%
! ATTCTTGAGACCAGTTTTTC+TGG - Chr4:73066485-73066504 None:intergenic 35.0%
! GGCGATTTTAGATTCTGTTT+TGG + Chr4:73066833-73066852 MsG0480022476.01.T01:CDS 35.0%
! TTCTTGAGACCAGTTTTTCT+GGG - Chr4:73066484-73066503 None:intergenic 35.0%
!!! GGGTGTTTTAAAAAAAGGAG+AGG + Chr4:73066627-73066646 MsG0480022476.01.T01:CDS 35.0%
AAAGGAGAGGAAAGAGAAAG+AGG + Chr4:73066640-73066659 MsG0480022476.01.T01:CDS 40.0%
ACTAAAGAGACGTCAAAGGA+TGG + Chr4:73066429-73066448 MsG0480022476.01.T01:CDS 40.0%
ACTCTCTCCAATTTCTGTGA+AGG - Chr4:73066399-73066418 None:intergenic 40.0%
ACTGTATATGAAGGACATGG+AGG + Chr4:73066606-73066625 MsG0480022476.01.T01:CDS 40.0%
ATGTCTTCAGAAGACATTGC+TGG - Chr4:73066243-73066262 None:intergenic 40.0%
CAAATCTTCTGGTGTAACTG+AGG + Chr4:73066770-73066789 MsG0480022476.01.T01:CDS 40.0%
CACATCAAACCCAGAAAAAC+TGG + Chr4:73066472-73066491 MsG0480022476.01.T01:CDS 40.0%
CACATCAAACCGATACAAAC+TGG + Chr4:73066199-73066218 MsG0480022476.01.T01:CDS 40.0%
CTAAAGAGACGTCAAAGGAT+GGG + Chr4:73066430-73066449 MsG0480022476.01.T01:CDS 40.0%
CTGTATATGAAGGACATGGA+GGG + Chr4:73066607-73066626 MsG0480022476.01.T01:CDS 40.0%
CTTGTTGACAACAACTCAGT+TGG + Chr4:73066084-73066103 MsG0480022476.01.T01:CDS 40.0%
GAGACTGTATATGAAGGACA+TGG + Chr4:73066603-73066622 MsG0480022476.01.T01:CDS 40.0%
GGAGAACACGAACTTGAAAA+AGG + Chr4:73066791-73066810 MsG0480022476.01.T01:CDS 40.0%
TGGTAGAACTTTCTTTCAGC+CGG - Chr4:73066731-73066750 None:intergenic 40.0%
TGTGAAGGAACATGTGATTC+TGG - Chr4:73066384-73066403 None:intergenic 40.0%
! AGCAATGTCTTCTGAAGACA+TGG + Chr4:73066242-73066261 MsG0480022476.01.T01:CDS 40.0%
! CAGGATCTTTTACACATACC+TGG - Chr4:73066899-73066918 None:intergenic 40.0%
! GATAAGTTGACAGATACAGC+TGG + Chr4:73066318-73066337 MsG0480022476.01.T01:CDS 40.0%
AGATTACGCCTGGTGTTTCA+GGG + Chr4:73066538-73066557 MsG0480022476.01.T01:CDS 45.0%
CAGAAGACATTGCTGGAACT+TGG - Chr4:73066509-73066528 None:intergenic 45.0%
GAAAGTTCTACCAGCAACTG+TGG + Chr4:73066738-73066757 MsG0480022476.01.T01:CDS 45.0%
GAGAGGAAAGAGAAAGAGGA+AGG + Chr4:73066644-73066663 MsG0480022476.01.T01:CDS 45.0%
GGATGGAACGAAAGATCTGA+TGG + Chr4:73066812-73066831 MsG0480022476.01.T01:CDS 45.0%
GGTAGAACTTTCTTTCAGCC+GGG - Chr4:73066730-73066749 None:intergenic 45.0%
GGTGAGGTTGTCAAATCTTC+TGG + Chr4:73066759-73066778 MsG0480022476.01.T01:CDS 45.0%
TCAATAGACCCTGAAACACC+AGG - Chr4:73066276-73066295 None:intergenic 45.0%
! AAACTGGTCTCATGAATGCC+AGG + Chr4:73066215-73066234 MsG0480022476.01.T01:CDS 45.0%
!! TCAAAGGATGGGTTATCTGC+TGG + Chr4:73066441-73066460 MsG0480022476.01.T01:CDS 45.0%
AGATCTCGCCTGGTGTTTCA+GGG + Chr4:73066265-73066284 MsG0480022476.01.T01:CDS 50.0%
CAGAAGACATTGCTGGAACC+TGG - Chr4:73066236-73066255 None:intergenic 50.0%
GAAGACATGGAGATTACGCC+TGG + Chr4:73066528-73066547 MsG0480022476.01.T01:CDS 50.0%
GAGATTACGCCTGGTGTTTC+AGG + Chr4:73066537-73066556 MsG0480022476.01.T01:CDS 50.0%
TTCTACCAGCAACTGTGGTG+AGG + Chr4:73066743-73066762 MsG0480022476.01.T01:CDS 50.0%
!! CAAAGGATGGGCTATCTGCT+GGG + Chr4:73066169-73066188 MsG0480022476.01.T01:CDS 50.0%
!! TCAAAGGATGGGCTATCTGC+TGG + Chr4:73066168-73066187 MsG0480022476.01.T01:CDS 50.0%
CTGTCGCAAGCATGATAGCC+AGG + Chr4:73066878-73066897 MsG0480022476.01.T01:CDS 55.0%
GAAGACATGGAGATCTCGCC+TGG + Chr4:73066255-73066274 MsG0480022476.01.T01:CDS 55.0%
GACAACCTCACCACAGTTGC+TGG - Chr4:73066751-73066770 None:intergenic 55.0%
GAGATCTCGCCTGGTGTTTC+AGG + Chr4:73066264-73066283 MsG0480022476.01.T01:CDS 55.0%
TCGGTTGACCCTGAAACACC+AGG - Chr4:73066549-73066568 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 73066078 73066932 73066078 ID=MsG0480022476.01;Name=MsG0480022476.01
Chr4 mRNA 73066078 73066932 73066078 ID=MsG0480022476.01.T01;Parent=MsG0480022476.01;Name=MsG0480022476.01.T01;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|284
Chr4 exon 73066078 73066932 73066078 ID=MsG0480022476.01.T01:exon:2773;Parent=MsG0480022476.01.T01
Chr4 CDS 73066078 73066932 73066078 ID=MsG0480022476.01.T01:cds;Parent=MsG0480022476.01.T01
Gene Sequence

>MsG0480022476.01.T01

ATGTGTCTTGTTGACAACAACTCAGTTGGACCAGAATTAGATGTTCCTTCACAGAAATTAGAGAGAGTTGAATCTTCTACTAAAGAGTTGTCAAAGGATGGGCTATCTGCTGGGAGTTTTACACATCAAACCGATACAAACTGGTCTCATGAATGCCAGGTTCCAGCAATGTCTTCTGAAGACATGGAGATCTCGCCTGGTGTTTCAGGGTCTATTGAACATGAGAAAGTTTTGAATATAGATAAGTTGACAGATACAGCTGGGAAAAGAGATGATTGTCATGTTGACAACGACTCAGTTGAACCAGAATCACATGTTCCTTCACAGAAATTGGAGAGAGTTGAATCTTCAACTAAAGAGACGTCAAAGGATGGGTTATCTGCTGGTAGTTTTACACATCAAACCCAGAAAAACTGGTCTCAAGAATGCCAAGTTCCAGCAATGTCTTCTGAAGACATGGAGATTACGCCTGGTGTTTCAGGGTCAACCGAACATGAGAAAGTTTTGAATGTAGATAAGTTGACAGAGACTGTATATGAAGGACATGGAGGGTGTTTTAAAAAAAGGAGAGGAAAGAGAAAGAGGAAGGATTGTGCCAGAAATATTAATGAAGCGAGTGTGAGAGAAAGTGATTTTTCAATTGATGTGTCCCGGCTGAAAGAAAGTTCTACCAGCAACTGTGGTGAGGTTGTCAAATCTTCTGGTGTAACTGAGGAGAACACGAACTTGAAAAAGGATGGAACGAAAGATCTGATGGCGATTTTAGATTCTGTTTTGGAAATTAAAGGTGCTTCTTGCTTCTGTCGCAAGCATGATAGCCAGGTATGTGTAAAAGATCCTGTTCACTCCCTTTAG

Protein sequence

>MsG0480022476.01.T01

MCLVDNNSVGPELDVPSQKLERVESSTKELSKDGLSAGSFTHQTDTNWSHECQVPAMSSEDMEISPGVSGSIEHEKVLNIDKLTDTAGKRDDCHVDNDSVEPESHVPSQKLERVESSTKETSKDGLSAGSFTHQTQKNWSQECQVPAMSSEDMEITPGVSGSTEHEKVLNVDKLTETVYEGHGGCFKKRRGKRKRKDCARNINEASVRESDFSIDVSRLKESSTSNCGEVVKSSGVTEENTNLKKDGTKDLMAILDSVLEIKGASCFCRKHDSQVCVKDPVHSL*