AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0174690


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0174690 MtrunA17_Chr1g0195361 95.152 165 8 0 1 165 1 165 4.69e-97 277
Msa0174690 MtrunA17_Chr1g0195381 49.618 131 61 2 32 157 23 153 7.91e-45 145
Msa0174690 MtrunA17_Chr7g0269431 45.122 164 57 3 1 164 2 132 2.78e-36 122
Msa0174690 MtrunA17_Chr6g0485071 32.877 146 92 4 4 144 5 149 4.60e-23 89.4
Msa0174690 MtrunA17_Chr8g0378491 34.746 118 72 3 30 144 27 142 1.12e-16 73.2
Msa0174690 MtrunA17_Chr7g0249501 31.148 122 78 3 34 151 42 161 8.00e-15 68.2
Msa0174690 MtrunA17_Chr7g0243261 30.526 95 64 2 35 128 56 149 3.13e-13 63.9
Msa0174690 MtrunA17_Chr8g0378501 27.434 113 79 2 34 144 32 143 1.55e-12 62.0
Msa0174690 MtrunA17_Chr7g0234601 31.304 115 72 4 34 144 37 148 1.98e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0174690 AT4G02360.1 53.947 152 60 1 3 154 9 150 9.43e-53 165
Msa0174690 AT4G02370.1 55.102 147 63 2 9 152 6 152 2.07e-52 164
Msa0174690 AT1G02816.1 58.594 128 52 1 32 158 31 158 3.87e-52 164
Msa0174690 AT1G02813.1 46.622 148 72 1 3 150 2 142 8.92e-43 139
Msa0174690 AT3G07460.1 31.481 162 95 7 4 161 2 151 1.41e-16 73.2
Msa0174690 AT3G07460.2 31.481 162 95 7 4 161 2 151 7.01e-16 73.2
Msa0174690 AT5G19590.1 29.661 118 81 1 29 144 27 144 4.80e-15 68.6
Msa0174690 AT5G19590.2 29.661 118 81 1 29 144 27 144 4.80e-15 68.6
Msa0174690 AT3G07470.3 30.714 140 83 5 8 143 13 142 2.36e-14 67.0
Msa0174690 AT3G07470.2 30.714 140 83 5 8 143 13 142 2.88e-14 67.0
Msa0174690 AT3G07470.1 30.822 146 86 6 3 143 7 142 3.16e-14 67.0
Msa0174690 AT5G37070.1 27.586 116 80 3 35 149 56 168 3.98e-13 63.9
Msa0174690 AT5G16380.1 33.333 141 84 6 6 143 9 142 4.10e-13 64.7
Msa0174690 AT3G08890.1 23.469 98 73 2 35 131 56 152 9.10e-11 57.8
Msa0174690 AT3G08890.2 23.469 98 73 2 35 131 56 152 9.10e-11 57.8

Find 30 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGATTACTTCTGCTGGTTT+TGG 0.248892 1_4:+60453571 Msa0174690:CDS
TGTTGAAGTTTCTTCAATTT+AGG 0.268351 1_4:+60453669 Msa0174690:CDS
ATTGACCTGTTTCTCTGTTA+AGG 0.276277 1_4:-60453357 None:intergenic
TTGACCTGTTTCTCTGTTAA+GGG 0.318122 1_4:-60453356 None:intergenic
GAATACCCAATTGCTCCTTT+TGG 0.335755 1_4:-60453334 None:intergenic
CTTCTTCCAAAAGGAGCAAT+TGG 0.352750 1_4:+60453328 Msa0174690:CDS
TCTGTTGGGATTACTTCTGC+TGG 0.400761 1_4:+60453565 Msa0174690:CDS
GAATGCCCTCAGTGTGGTTG+TGG 0.403933 1_4:+60453610 Msa0174690:CDS
TATTCCCTTAACAGAGAAAC+AGG 0.430305 1_4:+60453352 Msa0174690:CDS
GTGGTGTGATTTCGCAGAAT+AGG 0.437695 1_4:+60453452 Msa0174690:CDS
ATCAGCGTACGATCTTCTAA+TGG 0.446069 1_4:+60453285 Msa0174690:CDS
AACCACACTGAGGGCATTCA+AGG 0.446234 1_4:-60453606 None:intergenic
CCAATGGGTCTTCTTCCAAA+AGG 0.464533 1_4:+60453319 Msa0174690:CDS
GATAATCATCTGAGGTTAAA+CGG 0.466866 1_4:+60453643 Msa0174690:CDS
TACGATCTTCTAATGGAATA+CGG 0.472455 1_4:+60453292 Msa0174690:CDS
AATGCCCTCAGTGTGGTTGT+GGG 0.473689 1_4:+60453611 Msa0174690:CDS
TATTGTTGAGGTTTCTCGTA+AGG 0.475808 1_4:+60453525 Msa0174690:CDS
ATGGAATACGGTTTCCCAAT+GGG 0.477542 1_4:+60453304 Msa0174690:CDS
TTCTTCCAAAAGGAGCAATT+GGG 0.488774 1_4:+60453329 Msa0174690:CDS
CCACAAAAGCAAAATCTTGA+CGG 0.495996 1_4:-60453497 None:intergenic
ATTGTTGAGGTTTCTCGTAA+GGG 0.528099 1_4:+60453526 Msa0174690:CDS
GAATAGGCTTTATAAACTCA+AGG 0.537506 1_4:+60453468 Msa0174690:CDS
AATGGAATACGGTTTCCCAA+TGG 0.576232 1_4:+60453303 Msa0174690:CDS
TTTGTGGTTGAATATTGTTG+AGG 0.605032 1_4:+60453513 Msa0174690:CDS
AATAGGCTTTATAAACTCAA+GGG 0.613735 1_4:+60453469 Msa0174690:CDS
AGCTACAAATCTACTATCAG+TGG 0.618877 1_4:+60453433 Msa0174690:CDS
TTGATTGCGATAATCATCTG+AGG 0.632187 1_4:+60453635 Msa0174690:CDS
CAAACCCACAACCACACTGA+GGG 0.636398 1_4:-60453615 None:intergenic
TTCCTTGAATGCCCTCAGTG+TGG 0.651724 1_4:+60453604 Msa0174690:CDS
TCAAACCCACAACCACACTG+AGG 0.728360 1_4:-60453616 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGATATTGATTTTTCTGT+TGG + chr1_4:60453550-60453569 Msa0174690:CDS 20.0%
!!! ATGATATTGATTTTTCTGTT+GGG + chr1_4:60453551-60453570 Msa0174690:CDS 20.0%
!! AATAGGCTTTATAAACTCAA+GGG + chr1_4:60453469-60453488 Msa0174690:CDS 25.0%
TACGATCTTCTAATGGAATA+CGG + chr1_4:60453292-60453311 Msa0174690:CDS 30.0%
! GAATAGGCTTTATAAACTCA+AGG + chr1_4:60453468-60453487 Msa0174690:CDS 30.0%
TTTGTGGTTGAATATTGTTG+AGG + chr1_4:60453513-60453532 Msa0174690:CDS 30.0%
! GATAATCATCTGAGGTTAAA+CGG + chr1_4:60453643-60453662 Msa0174690:CDS 30.0%
!!! TAGCTTTTTCGGTTCGATTA+AGG - chr1_4:60453266-60453285 None:intergenic 35.0%
TTCTTCCAAAAGGAGCAATT+GGG + chr1_4:60453329-60453348 Msa0174690:CDS 35.0%
TATTCCCTTAACAGAGAAAC+AGG + chr1_4:60453352-60453371 Msa0174690:CDS 35.0%
! TTGACCTGTTTCTCTGTTAA+GGG - chr1_4:60453359-60453378 None:intergenic 35.0%
! ATTGACCTGTTTCTCTGTTA+AGG - chr1_4:60453360-60453379 None:intergenic 35.0%
AGCTACAAATCTACTATCAG+TGG + chr1_4:60453433-60453452 Msa0174690:CDS 35.0%
CCACAAAAGCAAAATCTTGA+CGG - chr1_4:60453500-60453519 None:intergenic 35.0%
TATTGTTGAGGTTTCTCGTA+AGG + chr1_4:60453525-60453544 Msa0174690:CDS 35.0%
ATTGTTGAGGTTTCTCGTAA+GGG + chr1_4:60453526-60453545 Msa0174690:CDS 35.0%
TTGATTGCGATAATCATCTG+AGG + chr1_4:60453635-60453654 Msa0174690:CDS 35.0%
! TCGTACGCTGATAGCTTTTT+CGG - chr1_4:60453277-60453296 None:intergenic 40.0%
ATCAGCGTACGATCTTCTAA+TGG + chr1_4:60453285-60453304 Msa0174690:CDS 40.0%
AATGGAATACGGTTTCCCAA+TGG + chr1_4:60453303-60453322 Msa0174690:CDS 40.0%
ATGGAATACGGTTTCCCAAT+GGG + chr1_4:60453304-60453323 Msa0174690:CDS 40.0%
!! CTTTTGGAAGAAGACCCATT+GGG - chr1_4:60453321-60453340 None:intergenic 40.0%
CTTCTTCCAAAAGGAGCAAT+TGG + chr1_4:60453328-60453347 Msa0174690:CDS 40.0%
! GAATACCCAATTGCTCCTTT+TGG - chr1_4:60453337-60453356 None:intergenic 40.0%
!! CCGTCAAGATTTTGCTTTTG+TGG + chr1_4:60453497-60453516 Msa0174690:CDS 40.0%
!! CCTTTTGGAAGAAGACCCAT+TGG - chr1_4:60453322-60453341 None:intergenic 45.0%
CCAATGGGTCTTCTTCCAAA+AGG + chr1_4:60453319-60453338 Msa0174690:CDS 45.0%
GTGGTGTGATTTCGCAGAAT+AGG + chr1_4:60453452-60453471 Msa0174690:CDS 45.0%
TCTGTTGGGATTACTTCTGC+TGG + chr1_4:60453565-60453584 Msa0174690:CDS 45.0%
!!! GGGATTACTTCTGCTGGTTT+TGG + chr1_4:60453571-60453590 Msa0174690:CDS 45.0%
TTCCTTGAATGCCCTCAGTG+TGG + chr1_4:60453604-60453623 Msa0174690:CDS 50.0%
!! AACCACACTGAGGGCATTCA+AGG - chr1_4:60453609-60453628 None:intergenic 50.0%
AATGCCCTCAGTGTGGTTGT+GGG + chr1_4:60453611-60453630 Msa0174690:CDS 50.0%
CAAACCCACAACCACACTGA+GGG - chr1_4:60453618-60453637 None:intergenic 50.0%
TCAAACCCACAACCACACTG+AGG - chr1_4:60453619-60453638 None:intergenic 50.0%
GAATGCCCTCAGTGTGGTTG+TGG + chr1_4:60453610-60453629 Msa0174690:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 60453193 60453690 60453193 ID=Msa0174690;Name=Msa0174690
chr1_4 mRNA 60453193 60453690 60453193 ID=Msa0174690-mRNA-1;Parent=Msa0174690;Name=Msa0174690-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|165
chr1_4 exon 60453193 60453690 60453193 ID=Msa0174690-mRNA-1:exon:19129;Parent=Msa0174690-mRNA-1
chr1_4 CDS 60453193 60453690 60453193 ID=Msa0174690-mRNA-1:cds;Parent=Msa0174690-mRNA-1
Gene Sequence

>Msa0174690

ATGTATTCGATTAAATTCATGTTGATTTTGTTAATCTCTCTAACAATATCATCATCGTTATCATCATGTACCTTAATCGAACCGAAAAAGCTATCAGCGTACGATCTTCTAATGGAATACGGTTTCCCAATGGGTCTTCTTCCAAAAGGAGCAATTGGGTATTCCCTTAACAGAGAAACAGGTCAATTTTCTGTGTATTTCGAGAAAACTTGTAGCTTTGTTATTGAAAGCTATACACTTAGCTACAAATCTACTATCAGTGGTGTGATTTCGCAGAATAGGCTTTATAAACTCAAGGGTGTTTCCGTCAAGATTTTGCTTTTGTGGTTGAATATTGTTGAGGTTTCTCGTAAGGGTAATGATATTGATTTTTCTGTTGGGATTACTTCTGCTGGTTTTGGAGTTGAGAATTTCCTTGAATGCCCTCAGTGTGGTTGTGGGTTTGATTGCGATAATCATCTGAGGTTAAACGGTGATGTTGAAGTTTCTTCAATTTAG

Protein sequence

>Msa0174690

MYSIKFMLILLISLTISSSLSSCTLIEPKKLSAYDLLMEYGFPMGLLPKGAIGYSLNRETGQFSVYFEKTCSFVIESYTLSYKSTISGVISQNRLYKLKGVSVKILLLWLNIVEVSRKGNDIDFSVGITSAGFGVENFLECPQCGCGFDCDNHLRLNGDVEVSSI*