AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0128650


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0128650 MtrunA17_Chr1g0195361 96.875 128 4 0 1 128 38 165 4.50e-89 255
Msa0128650 MtrunA17_Chr1g0195381 50.407 123 56 2 3 120 31 153 9.51e-43 138
Msa0128650 MtrunA17_Chr7g0269431 49.587 121 31 2 2 122 37 127 1.15e-32 112
Msa0128650 MtrunA17_Chr6g0485071 33.333 108 69 2 3 107 42 149 5.66e-20 80.1
Msa0128650 MtrunA17_Chr7g0249501 30.172 116 75 3 4 115 49 162 1.73e-13 63.5
Msa0128650 MtrunA17_Chr7g0243261 29.670 91 62 2 2 91 60 149 2.09e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0128650 AT1G02816.1 59.167 120 48 1 3 121 39 158 2.55e-49 155
Msa0128650 AT4G02360.1 62.609 115 43 0 3 117 36 150 1.70e-48 152
Msa0128650 AT4G02370.1 60.526 114 44 1 3 115 39 152 2.98e-48 152
Msa0128650 AT1G02813.1 51.351 111 54 0 3 113 32 142 1.70e-38 127
Msa0128650 AT3G07460.1 36.000 125 65 6 4 124 38 151 8.39e-15 67.4
Msa0128650 AT3G07460.2 36.000 125 65 6 4 124 38 151 2.92e-14 67.4
Msa0128650 AT5G19590.1 29.907 107 73 1 3 107 38 144 5.01e-14 64.7
Msa0128650 AT5G19590.2 29.907 107 73 1 3 107 38 144 5.01e-14 64.7
Msa0128650 AT5G37070.1 26.786 112 78 3 2 112 60 168 2.67e-12 60.8
Msa0128650 AT5G19860.1 30.275 109 71 3 2 107 42 148 1.69e-11 58.9
Msa0128650 AT3G07470.3 32.710 107 65 4 4 106 39 142 5.18e-11 57.0
Msa0128650 AT3G07470.2 32.710 107 65 4 4 106 39 142 7.01e-11 57.0
Msa0128650 AT3G07470.1 32.710 107 65 4 4 106 39 142 7.63e-11 57.0

Find 29 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGATTACTTCTGCTGGTTT+TGG 0.248892 1_3:+58484678 Msa0128650:CDS
TGTTGAAGTTTCTTCAATTT+AGG 0.268351 1_3:+58484776 Msa0128650:CDS
ATTGACCTGTTTCTCTGTTA+AGG 0.276277 1_3:-58484464 None:intergenic
TTGACCTGTTTCTCTGTTAA+GGG 0.318122 1_3:-58484463 None:intergenic
GAATACCCAATTGCTCCTTT+TGG 0.335755 1_3:-58484441 None:intergenic
CTTCTTCCAAAAGGAGCAAT+TGG 0.352750 1_3:+58484435 Msa0128650:CDS
TCTGTTGGGATTACTTCTGC+TGG 0.400761 1_3:+58484672 Msa0128650:CDS
GAATGCCCTCAGTGTGGTTG+TGG 0.403933 1_3:+58484717 Msa0128650:CDS
TATTCCCTTAACAGAGAAAC+AGG 0.430305 1_3:+58484459 Msa0128650:CDS
GTGGTGTGATTTCGCAGAAT+AGG 0.437695 1_3:+58484559 Msa0128650:CDS
AACCACACTGAGGGCATTCA+AGG 0.446234 1_3:-58484713 None:intergenic
CCAATGGGTCTTCTTCCAAA+AGG 0.464533 1_3:+58484426 Msa0128650:CDS
GATAATCATCTGAGGTTAAA+CGG 0.466866 1_3:+58484750 Msa0128650:CDS
TACGATCTTCTAATGGAATA+CGG 0.472455 1_3:+58484399 None:intergenic
AATGCCCTCAGTGTGGTTGT+GGG 0.473689 1_3:+58484718 Msa0128650:CDS
TATTGTTGAGGTTTCTCGTA+AGG 0.475808 1_3:+58484632 Msa0128650:CDS
ATGGAATACGGTTTCCCAAT+GGG 0.477542 1_3:+58484411 Msa0128650:CDS
TTCTTCCAAAAGGAGCAATT+GGG 0.488774 1_3:+58484436 Msa0128650:CDS
CCACAAAAGCAAAATCTTGA+CGG 0.495996 1_3:-58484604 None:intergenic
ATTGTTGAGGTTTCTCGTAA+GGG 0.528099 1_3:+58484633 Msa0128650:CDS
GAATAGGCTTTATAAACTCA+AGG 0.537506 1_3:+58484575 Msa0128650:CDS
AATGGAATACGGTTTCCCAA+TGG 0.576232 1_3:+58484410 None:intergenic
TTTGTGGTTGAATATTGTTG+AGG 0.605032 1_3:+58484620 Msa0128650:CDS
AATAGGCTTTATAAACTCAA+GGG 0.613735 1_3:+58484576 Msa0128650:CDS
AGCTACAAATCTACTATCAG+TGG 0.618877 1_3:+58484540 Msa0128650:CDS
TTGATTGCGATAATCATCTG+AGG 0.632187 1_3:+58484742 Msa0128650:CDS
CAAACCCACAACCACACTGA+GGG 0.636398 1_3:-58484722 None:intergenic
TTCCTTGAATGCCCTCAGTG+TGG 0.651724 1_3:+58484711 Msa0128650:CDS
TCAAACCCACAACCACACTG+AGG 0.728360 1_3:-58484723 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGATATTGATTTTTCTGT+TGG + chr1_3:58484657-58484676 Msa0128650:CDS 20.0%
!!! ATGATATTGATTTTTCTGTT+GGG + chr1_3:58484658-58484677 Msa0128650:CDS 20.0%
!! AATAGGCTTTATAAACTCAA+GGG + chr1_3:58484576-58484595 Msa0128650:CDS 25.0%
! GAATAGGCTTTATAAACTCA+AGG + chr1_3:58484575-58484594 Msa0128650:CDS 30.0%
TTTGTGGTTGAATATTGTTG+AGG + chr1_3:58484620-58484639 Msa0128650:CDS 30.0%
! GATAATCATCTGAGGTTAAA+CGG + chr1_3:58484750-58484769 Msa0128650:CDS 30.0%
TTCTTCCAAAAGGAGCAATT+GGG + chr1_3:58484436-58484455 Msa0128650:CDS 35.0%
TATTCCCTTAACAGAGAAAC+AGG + chr1_3:58484459-58484478 Msa0128650:CDS 35.0%
! TTGACCTGTTTCTCTGTTAA+GGG - chr1_3:58484466-58484485 None:intergenic 35.0%
! ATTGACCTGTTTCTCTGTTA+AGG - chr1_3:58484467-58484486 None:intergenic 35.0%
AGCTACAAATCTACTATCAG+TGG + chr1_3:58484540-58484559 Msa0128650:CDS 35.0%
CCACAAAAGCAAAATCTTGA+CGG - chr1_3:58484607-58484626 None:intergenic 35.0%
TATTGTTGAGGTTTCTCGTA+AGG + chr1_3:58484632-58484651 Msa0128650:CDS 35.0%
ATTGTTGAGGTTTCTCGTAA+GGG + chr1_3:58484633-58484652 Msa0128650:CDS 35.0%
TTGATTGCGATAATCATCTG+AGG + chr1_3:58484742-58484761 Msa0128650:CDS 35.0%
ATGGAATACGGTTTCCCAAT+GGG + chr1_3:58484411-58484430 Msa0128650:CDS 40.0%
!! CTTTTGGAAGAAGACCCATT+GGG - chr1_3:58484428-58484447 None:intergenic 40.0%
CTTCTTCCAAAAGGAGCAAT+TGG + chr1_3:58484435-58484454 Msa0128650:CDS 40.0%
! GAATACCCAATTGCTCCTTT+TGG - chr1_3:58484444-58484463 None:intergenic 40.0%
!! CCGTCAAGATTTTGCTTTTG+TGG + chr1_3:58484604-58484623 Msa0128650:CDS 40.0%
!! CCTTTTGGAAGAAGACCCAT+TGG - chr1_3:58484429-58484448 None:intergenic 45.0%
CCAATGGGTCTTCTTCCAAA+AGG + chr1_3:58484426-58484445 Msa0128650:CDS 45.0%
GTGGTGTGATTTCGCAGAAT+AGG + chr1_3:58484559-58484578 Msa0128650:CDS 45.0%
TCTGTTGGGATTACTTCTGC+TGG + chr1_3:58484672-58484691 Msa0128650:CDS 45.0%
!!! GGGATTACTTCTGCTGGTTT+TGG + chr1_3:58484678-58484697 Msa0128650:CDS 45.0%
TTCCTTGAATGCCCTCAGTG+TGG + chr1_3:58484711-58484730 Msa0128650:CDS 50.0%
!! AACCACACTGAGGGCATTCA+AGG - chr1_3:58484716-58484735 None:intergenic 50.0%
AATGCCCTCAGTGTGGTTGT+GGG + chr1_3:58484718-58484737 Msa0128650:CDS 50.0%
CAAACCCACAACCACACTGA+GGG - chr1_3:58484725-58484744 None:intergenic 50.0%
TCAAACCCACAACCACACTG+AGG - chr1_3:58484726-58484745 None:intergenic 50.0%
GAATGCCCTCAGTGTGGTTG+TGG + chr1_3:58484717-58484736 Msa0128650:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 58484411 58484797 58484411 ID=Msa0128650;Name=Msa0128650
chr1_3 mRNA 58484411 58484797 58484411 ID=Msa0128650-mRNA-1;Parent=Msa0128650;Name=Msa0128650-mRNA-1;_AED=0.31;_eAED=0.31;_QI=0|-1|0|1|-1|1|1|0|128
chr1_3 exon 58484411 58484797 58484411 ID=Msa0128650-mRNA-1:exon:18780;Parent=Msa0128650-mRNA-1
chr1_3 CDS 58484411 58484797 58484411 ID=Msa0128650-mRNA-1:cds;Parent=Msa0128650-mRNA-1
Gene Sequence

>Msa0128650

ATGGAATACGGTTTCCCAATGGGTCTTCTTCCAAAAGGAGCAATTGGGTATTCCCTTAACAGAGAAACAGGTCAATTTTCTGTGTATTTCGAGAAAACTTGTAGCTTTGTTATTGAAAGCTATACACTTAGCTACAAATCTACTATCAGTGGTGTGATTTCGCAGAATAGGCTTTATAAACTCAAGGGTGTTTCCGTCAAGATTTTGCTTTTGTGGTTGAATATTGTTGAGGTTTCTCGTAAGGGTAATGATATTGATTTTTCTGTTGGGATTACTTCTGCTGGTTTTGGAGTTGAGAATTTCCTTGAATGCCCTCAGTGTGGTTGTGGGTTTGATTGCGATAATCATCTGAGGTTAAACGGTGATGTTGAAGTTTCTTCAATTTAG

Protein sequence

>Msa0128650

MEYGFPMGLLPKGAIGYSLNRETGQFSVYFEKTCSFVIESYTLSYKSTISGVISQNRLYKLKGVSVKILLLWLNIVEVSRKGNDIDFSVGITSAGFGVENFLECPQCGCGFDCDNHLRLNGDVEVSSI*