Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0004950 | Msa0429210 | 0.824668 | 7.124324e-54 | -8.615850e-47 |
| Msa0004980 | Msa0429210 | 0.810542 | 1.094471e-50 | -8.615850e-47 |
| Msa0016670 | Msa0429210 | 0.803517 | 3.357018e-49 | -8.615850e-47 |
| Msa0027260 | Msa0429210 | 0.813713 | 2.226973e-51 | -8.615850e-47 |
| Msa0030310 | Msa0429210 | 0.802842 | 4.631124e-49 | -8.615850e-47 |
| Msa0032840 | Msa0429210 | -0.808835 | 2.548004e-50 | -8.615850e-47 |
| Msa0073670 | Msa0429210 | 0.812815 | 3.506657e-51 | -8.615850e-47 |
| Msa0074320 | Msa0429210 | 0.805115 | 1.559636e-49 | -8.615850e-47 |
| Msa0094950 | Msa0429210 | 0.807216 | 5.633210e-50 | -8.615850e-47 |
| Msa0128860 | Msa0429210 | 0.821225 | 4.522035e-53 | -8.615850e-47 |
| Msa0167480 | Msa0429210 | 0.820416 | 6.939586e-53 | -8.615850e-47 |
| Msa0174730 | Msa0429210 | 0.820922 | 5.309063e-53 | -8.615850e-47 |
| Msa0187930 | Msa0429210 | 0.822539 | 2.244249e-53 | -8.615850e-47 |
| Msa0194540 | Msa0429210 | 0.827913 | 1.202779e-54 | -8.615850e-47 |
| Msa0199690 | Msa0429210 | 0.806875 | 6.650349e-50 | -8.615850e-47 |
| Msa0199880 | Msa0429210 | 0.807548 | 4.789736e-50 | -8.615850e-47 |
| Msa0230550 | Msa0429210 | -0.807161 | 5.785745e-50 | -8.615850e-47 |
| Msa0369220 | Msa0429210 | 0.802728 | 4.888121e-49 | -8.615850e-47 |
| Msa0370160 | Msa0429210 | -0.815223 | 1.031774e-51 | -8.615850e-47 |
| Msa0429210 | Msa0437690 | -0.827073 | 1.913112e-54 | -8.615850e-47 |
| Msa0429210 | Msa0443390 | 0.816604 | 5.077515e-52 | -8.615850e-47 |
| Msa0429210 | Msa0469910 | -0.803265 | 3.785222e-49 | -8.615850e-47 |
| Msa0429210 | Msa0508590 | 0.829113 | 6.172626e-55 | -8.615850e-47 |
| Msa0429210 | Msa0508630 | 0.820575 | 6.380721e-53 | -8.615850e-47 |
| Msa0429210 | Msa0509950 | -0.822402 | 2.415048e-53 | -8.615850e-47 |
| Msa0429210 | Msa0525340 | 0.805909 | 1.063065e-49 | -8.615850e-47 |
| Msa0429210 | Msa0526540 | 0.830593 | 2.690544e-55 | -8.615850e-47 |
| Msa0429210 | Msa0576940 | 0.837548 | 4.872252e-57 | -8.615850e-47 |
| Msa0429210 | Msa0581070 | 0.805845 | 1.096350e-49 | -8.615850e-47 |
| Msa0429210 | Msa0616240 | 0.808846 | 2.533685e-50 | -8.615850e-47 |
| Msa0429210 | Msa0647380 | 0.813595 | 2.364074e-51 | -8.615850e-47 |
| Msa0429210 | Msa0669850 | 0.802255 | 6.119992e-49 | -8.615850e-47 |
| Msa0429210 | Msa0677290 | 0.818579 | 1.821314e-52 | -8.615850e-47 |
| Msa0429210 | Msa0678860 | 0.815399 | 9.432104e-52 | -8.615850e-47 |
| Msa0429210 | Msa0680240 | 0.816699 | 4.833092e-52 | -8.615850e-47 |
| Msa0429210 | Msa0687630 | 0.823166 | 1.603354e-53 | -8.615850e-47 |
| Msa0429210 | Msa0699570 | 0.810651 | 1.036739e-50 | -8.615850e-47 |
| Msa0429210 | Msa0712310 | 0.812618 | 3.871344e-51 | -8.615850e-47 |
| Msa0429210 | Msa0718740 | 0.805657 | 1.200662e-49 | -8.615850e-47 |
| Msa0429210 | Msa0747700 | 0.808337 | 3.254846e-50 | -8.615850e-47 |
| Msa0429210 | Msa0755340 | 0.803911 | 2.780798e-49 | -8.615850e-47 |
| Msa0429210 | Msa0795030 | 0.808730 | 2.683481e-50 | -8.615850e-47 |
| Msa0429210 | Msa0813720 | 0.836317 | 1.004572e-56 | -8.615850e-47 |
| Msa0429210 | Msa0830990 | 0.817172 | 3.785714e-52 | -8.615850e-47 |
| Msa0429210 | Msa0900630 | 0.818707 | 1.703261e-52 | -8.615850e-47 |
| Msa0429210 | Msa0926460 | 0.802269 | 6.077574e-49 | -8.615850e-47 |
| Msa0429210 | Msa0957450 | -0.806103 | 9.677823e-50 | -8.615850e-47 |
| Msa0429210 | Msa0974810 | 0.823345 | 1.456350e-53 | -8.615850e-47 |
| Msa0429210 | Msa1008060 | -0.800605 | 1.332259e-48 | -8.615850e-47 |
| Msa0429210 | Msa1019240 | 0.842230 | 2.936174e-58 | -8.615850e-47 |
| Msa0429210 | Msa1019260 | 0.841368 | 4.957571e-58 | -8.615850e-47 |
| Msa0429210 | Msa1054240 | 0.800944 | 1.136535e-48 | -8.615850e-47 |
| Msa0429210 | Msa1054730 | 0.805794 | 1.123988e-49 | -8.615850e-47 |
| Msa0429210 | Msa1069660 | -0.807540 | 4.808469e-50 | -8.615850e-47 |
| Msa0429210 | Msa1082700 | 0.801034 | 1.089377e-48 | -8.615850e-47 |
| Msa0429210 | Msa1084470 | 0.801799 | 7.593893e-49 | -8.615850e-47 |
| Msa0429210 | Msa1094420 | 0.815972 | 7.028933e-52 | -8.615850e-47 |
| Msa0429210 | Msa1104140 | 0.814579 | 1.434181e-51 | -8.615850e-47 |
| Msa0429210 | Msa1104210 | 0.843358 | 1.471778e-58 | -8.615850e-47 |
| Msa0429210 | Msa1126120 | -0.803726 | 3.037329e-49 | -8.615850e-47 |
| Msa0429210 | Msa1126510 | 0.820309 | 7.340927e-53 | -8.615850e-47 |
| Msa0429210 | Msa1126770 | 0.800020 | 1.752930e-48 | -8.615850e-47 |
| Msa0429210 | Msa1138870 | 0.803373 | 3.595200e-49 | -8.615850e-47 |
| Msa0429210 | Msa1166010 | 0.841689 | 4.079938e-58 | -8.615850e-47 |
| Msa0429210 | Msa1166860 | 0.819491 | 1.129240e-52 | -8.615850e-47 |
| Msa0429210 | Msa1166870 | 0.834006 | 3.847120e-56 | -8.615850e-47 |
| Msa0429210 | Msa1276930 | 0.813315 | 2.723441e-51 | -8.615850e-47 |
| Msa0429210 | Msa1310690 | 0.801038 | 1.086963e-48 | -8.615850e-47 |
| Msa0429210 | Msa1335940 | 0.819709 | 1.007354e-52 | -8.615850e-47 |
| Msa0429210 | Msa1349570 | 0.809978 | 1.448465e-50 | -8.615850e-47 |
| Msa0429210 | Msa1363330 | 0.828393 | 9.215795e-55 | -8.615850e-47 |
| Msa0429210 | Msa1365150 | 0.815851 | 7.480657e-52 | -8.615850e-47 |
| Msa0429210 | Msa1367880 | 0.800990 | 1.111755e-48 | -8.615850e-47 |
| Msa0429210 | Msa1379760 | 0.830606 | 2.671216e-55 | -8.615850e-47 |
| Msa0429210 | Msa1386600 | 0.827664 | 1.380545e-54 | -8.615850e-47 |
| Msa0429210 | Msa1404190 | 0.819638 | 1.045744e-52 | -8.615850e-47 |
| Msa0429210 | Msa1406040 | 0.811139 | 8.130144e-51 | -8.615850e-47 |
| Msa0429210 | Msa1438640 | 0.805706 | 1.172490e-49 | -8.615850e-47 |
| Msa0429210 | Msa1442850 | 0.800044 | 1.733707e-48 | -8.615850e-47 |
| Msa0429210 | Msa1448690 | 0.812725 | 3.669030e-51 | -8.615850e-47 |
| Msa0429210 | Msa1450280 | 0.820783 | 5.714029e-53 | -8.615850e-47 |
| Msa0429210 | Msa1461490 | 0.818015 | 2.443362e-52 | -8.615850e-47 |
| Msa0241800 | Msa0429210 | 0.801897 | 7.248715e-49 | -8.615850e-47 |
| Msa0244780 | Msa0429210 | 0.819979 | 8.740461e-53 | -8.615850e-47 |
| Msa0260240 | Msa0429210 | 0.811575 | 6.538134e-51 | -8.615850e-47 |
| Msa0260280 | Msa0429210 | 0.811243 | 7.718343e-51 | -8.615850e-47 |
| Msa0262310 | Msa0429210 | 0.804289 | 2.320720e-49 | -8.615850e-47 |
| Msa0268130 | Msa0429210 | 0.822724 | 2.032096e-53 | -8.615850e-47 |
| Msa0280430 | Msa0429210 | 0.818015 | 2.443205e-52 | -8.615850e-47 |
| Msa0298990 | Msa0429210 | 0.817022 | 4.091186e-52 | -8.615850e-47 |
| Msa0304260 | Msa0429210 | 0.809346 | 1.980433e-50 | -8.615850e-47 |
| Msa0337650 | Msa0429210 | 0.827567 | 1.456467e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGAGTTCCATTGCTTAAT+TGG | 0.222933 | 3_2:+80444634 | None:intergenic |
| TCTTACGTGGACAATTGATA+TGG | 0.322055 | 3_2:+80444872 | None:intergenic |
| CTAAAAGACGTTATAAATCT+TGG | 0.340563 | 3_2:-80444849 | Msa0429210:five_prime_UTR |
| AGTACTTTGATGTGTTTGTT+AGG | 0.349467 | 3_2:-80444440 | Msa0429210:CDS |
| TGACAGTGACAGTGAGATAA+TGG | 0.395569 | 3_2:-80444403 | Msa0429210:CDS |
| TGGTTTAGTACAATCGTTAC+CGG | 0.434065 | 3_2:-80444767 | Msa0429210:intron |
| GAAATTACAATGAGTTAACT+TGG | 0.462389 | 3_2:+80444580 | None:intergenic |
| ATTCCACTTGATTCTCCATC+CGG | 0.489687 | 3_2:+80444906 | None:intergenic |
| GGATGGAGAATCAAGTGGAA+TGG | 0.607473 | 3_2:-80444904 | Msa0429210:five_prime_UTR |
| TCTGCTCTAGACTCCGCCGT+CGG | 0.612404 | 3_2:+80444955 | None:intergenic |
| TATCCGGATGGAGAATCAAG+TGG | 0.622649 | 3_2:-80444909 | Msa0429210:five_prime_UTR |
| CAATCAAGCCAATTAAGCAA+TGG | 0.626444 | 3_2:-80444642 | Msa0429210:CDS |
| AAGATTCACATGCTTCAAAG+AGG | 0.642884 | 3_2:-80445007 | Msa0429210:five_prime_UTR |
| AGATTCACATGCTTCAAAGA+GGG | 0.654356 | 3_2:-80445006 | Msa0429210:five_prime_UTR |
| TGACAAAAGACGTCAACCGA+CGG | 0.667263 | 3_2:-80444971 | Msa0429210:five_prime_UTR |
| CAAAAGACGTCAACCGACGG+CGG | 0.706901 | 3_2:-80444968 | Msa0429210:five_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAAAGTTAAATAAATGAT+AGG | + | chr3_2:80444725-80444744 | None:intergenic | 15.0% |
| !! | AAATTGAAAAAATCTCAAGA+TGG | - | chr3_2:80444639-80444658 | Msa0429210:CDS | 20.0% |
| ! | TATGTTGTCCAAAAAATATC+CGG | - | chr3_2:80444501-80444520 | Msa0429210:intron | 25.0% |
| ! | CTAAAAGACGTTATAAATCT+TGG | - | chr3_2:80444577-80444596 | Msa0429210:intron | 25.0% |
| ! | GATAAAAGAAAAATACGTAC+CGG | + | chr3_2:80444681-80444700 | None:intergenic | 25.0% |
| ! | GAAATTACAATGAGTTAACT+TGG | + | chr3_2:80444849-80444868 | None:intergenic | 25.0% |
| !! | AAAAAACACATCAAAGTACT+TGG | + | chr3_2:80444908-80444927 | None:intergenic | 25.0% |
| GCTTGAAAAAGAAGCTAAAA+AGG | - | chr3_2:80444599-80444618 | Msa0429210:CDS | 30.0% | |
| ! | ACAGTTTTCATGTGTGTATT+AGG | - | chr3_2:80444869-80444888 | Msa0429210:five_prime_UTR | 30.0% |
| AGTACTTTGATGTGTTTGTT+AGG | - | chr3_2:80444986-80445005 | Msa0429210:five_prime_UTR | 30.0% | |
| AAGATTCACATGCTTCAAAG+AGG | - | chr3_2:80444419-80444438 | Msa0429210:CDS | 35.0% | |
| AGATTCACATGCTTCAAAGA+GGG | - | chr3_2:80444420-80444439 | Msa0429210:CDS | 35.0% | |
| TTGTCCAAAAAATATCCGGA+TGG | - | chr3_2:80444505-80444524 | Msa0429210:intron | 35.0% | |
| !!! | TTCTCCATCCGGATATTTTT+TGG | + | chr3_2:80444512-80444531 | None:intergenic | 35.0% |
| TCTTACGTGGACAATTGATA+TGG | + | chr3_2:80444557-80444576 | None:intergenic | 35.0% | |
| !!! | TAACGTCTTTTAGTCTTACG+TGG | + | chr3_2:80444570-80444589 | None:intergenic | 35.0% |
| TGGTTTAGTACAATCGTTAC+CGG | - | chr3_2:80444659-80444678 | Msa0429210:CDS | 35.0% | |
| CAATCAAGCCAATTAAGCAA+TGG | - | chr3_2:80444784-80444803 | Msa0429210:CDS | 35.0% | |
| GTTGAGTTCCATTGCTTAAT+TGG | + | chr3_2:80444795-80444814 | None:intergenic | 35.0% | |
| AACAAACACATCAAAGGACT+TGG | + | chr3_2:80444947-80444966 | None:intergenic | 35.0% | |
| AGTCCTTTGATGTGTTTGTT+AGG | - | chr3_2:80444947-80444966 | Msa0429210:five_prime_UTR | 35.0% | |
| TCTCCTAACAAACACATCAA+AGG | + | chr3_2:80444953-80444972 | None:intergenic | 35.0% | |
| ATTCCACTTGATTCTCCATC+CGG | + | chr3_2:80444523-80444542 | None:intergenic | 40.0% | |
| TGACAGTGACAGTGAGATAA+TGG | - | chr3_2:80445023-80445042 | Msa0429210:five_prime_UTR | 40.0% | |
| TGACAAAAGACGTCAACCGA+CGG | - | chr3_2:80444455-80444474 | Msa0429210:CDS | 45.0% | |
| TATCCGGATGGAGAATCAAG+TGG | - | chr3_2:80444517-80444536 | Msa0429210:intron | 45.0% | |
| GGATGGAGAATCAAGTGGAA+TGG | - | chr3_2:80444522-80444541 | Msa0429210:intron | 45.0% | |
| CAAAAGACGTCAACCGACGG+CGG | - | chr3_2:80444458-80444477 | Msa0429210:CDS | 55.0% | |
| ! | TCTGCTCTAGACTCCGCCGT+CGG | + | chr3_2:80444474-80444493 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 80444398 | 80445050 | 80444398 | ID=Msa0429210;Name=Msa0429210 |
| chr3_2 | mRNA | 80444398 | 80445050 | 80444398 | ID=Msa0429210-mRNA-1;Parent=Msa0429210;Name=Msa0429210-mRNA-1;_AED=0.19;_eAED=0.19;_QI=229|0.5|0.33|1|1|1|3|0|79 |
| chr3_2 | exon | 80444398 | 80444479 | 80444398 | ID=Msa0429210-mRNA-1:exon:22054;Parent=Msa0429210-mRNA-1 |
| chr3_2 | exon | 80444588 | 80444691 | 80444588 | ID=Msa0429210-mRNA-1:exon:22053;Parent=Msa0429210-mRNA-1 |
| chr3_2 | exon | 80444768 | 80445050 | 80444768 | ID=Msa0429210-mRNA-1:exon:22052;Parent=Msa0429210-mRNA-1 |
| chr3_2 | five_prime_UTR | 80444822 | 80445050 | 80444822 | ID=Msa0429210-mRNA-1:five_prime_utr;Parent=Msa0429210-mRNA-1 |
| chr3_2 | CDS | 80444768 | 80444821 | 80444768 | ID=Msa0429210-mRNA-1:cds;Parent=Msa0429210-mRNA-1 |
| chr3_2 | CDS | 80444588 | 80444691 | 80444588 | ID=Msa0429210-mRNA-1:cds;Parent=Msa0429210-mRNA-1 |
| chr3_2 | CDS | 80444398 | 80444479 | 80444398 | ID=Msa0429210-mRNA-1:cds;Parent=Msa0429210-mRNA-1 |
| Gene Sequence |
| Protein sequence |
