Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071407.t1 | XP_003602045.1 | 98.8 | 160 | 2 | 0 | 1 | 160 | 277 | 436 | 6.50E-83 | 316.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071407.t1 | Q42593 | 78.0 | 159 | 31 | 1 | 1 | 159 | 262 | 416 | 2.6e-64 | 246.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071407.t1 | G7J4Y2 | 98.8 | 160 | 2 | 0 | 1 | 160 | 277 | 436 | 4.7e-83 | 316.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049149 | MS.gene071407 | 0.829352 | 5.40E-55 | -1.69E-46 |
| MS.gene049150 | MS.gene071407 | 0.836522 | 8.91E-57 | -1.69E-46 |
| MS.gene049151 | MS.gene071407 | 0.82774 | 1.32E-54 | -1.69E-46 |
| MS.gene049153 | MS.gene071407 | 0.835382 | 1.73E-56 | -1.69E-46 |
| MS.gene049260 | MS.gene071407 | 0.837123 | 6.26E-57 | -1.69E-46 |
| MS.gene049346 | MS.gene071407 | 0.839911 | 1.19E-57 | -1.69E-46 |
| MS.gene049378 | MS.gene071407 | 0.824433 | 8.09E-54 | -1.69E-46 |
| MS.gene049491 | MS.gene071407 | 0.803225 | 3.86E-49 | -1.69E-46 |
| MS.gene049599 | MS.gene071407 | -0.861219 | 1.20E-63 | -1.69E-46 |
| MS.gene049758 | MS.gene071407 | 0.918183 | 2.13E-86 | -1.69E-46 |
| MS.gene049910 | MS.gene071407 | 0.870284 | 1.64E-66 | -1.69E-46 |
| MS.gene049915 | MS.gene071407 | 0.84152 | 4.52E-58 | -1.69E-46 |
| MS.gene050076 | MS.gene071407 | 0.816556 | 5.20E-52 | -1.69E-46 |
| MS.gene050128 | MS.gene071407 | 0.8356 | 1.53E-56 | -1.69E-46 |
| MS.gene050226 | MS.gene071407 | 0.803321 | 3.69E-49 | -1.69E-46 |
| MS.gene050227 | MS.gene071407 | 0.811484 | 6.84E-51 | -1.69E-46 |
| MS.gene050228 | MS.gene071407 | 0.804153 | 2.48E-49 | -1.69E-46 |
| MS.gene050301 | MS.gene071407 | 0.877634 | 5.37E-69 | -1.69E-46 |
| MS.gene050330 | MS.gene071407 | -0.827089 | 1.90E-54 | -1.69E-46 |
| MS.gene050397 | MS.gene071407 | 0.837914 | 3.92E-57 | -1.69E-46 |
| MS.gene050432 | MS.gene071407 | 0.831066 | 2.06E-55 | -1.69E-46 |
| MS.gene050464 | MS.gene071407 | 0.849935 | 2.35E-60 | -1.69E-46 |
| MS.gene050529 | MS.gene071407 | 0.846298 | 2.37E-59 | -1.69E-46 |
| MS.gene050558 | MS.gene071407 | 0.834517 | 2.86E-56 | -1.69E-46 |
| MS.gene050623 | MS.gene071407 | -0.824232 | 9.03E-54 | -1.69E-46 |
| MS.gene050642 | MS.gene071407 | 0.850218 | 1.96E-60 | -1.69E-46 |
| MS.gene050670 | MS.gene071407 | 0.806147 | 9.47E-50 | -1.69E-46 |
| MS.gene050762 | MS.gene071407 | 0.800301 | 1.54E-48 | -1.69E-46 |
| MS.gene050763 | MS.gene071407 | 0.804091 | 2.55E-49 | -1.69E-46 |
| MS.gene050820 | MS.gene071407 | 0.806755 | 7.05E-50 | -1.69E-46 |
| MS.gene05101 | MS.gene071407 | 0.820742 | 5.84E-53 | -1.69E-46 |
| MS.gene05119 | MS.gene071407 | -0.877884 | 4.39E-69 | -1.69E-46 |
| MS.gene051471 | MS.gene071407 | 0.82593 | 3.58E-54 | -1.69E-46 |
| MS.gene051535 | MS.gene071407 | 0.805926 | 1.05E-49 | -1.69E-46 |
| MS.gene051582 | MS.gene071407 | 0.809581 | 1.76E-50 | -1.69E-46 |
| MS.gene051662 | MS.gene071407 | 0.845337 | 4.32E-59 | -1.69E-46 |
| MS.gene051663 | MS.gene071407 | 0.846907 | 1.62E-59 | -1.69E-46 |
| MS.gene051860 | MS.gene071407 | 0.866341 | 3.06E-65 | -1.69E-46 |
| MS.gene051983 | MS.gene071407 | 0.856218 | 3.73E-62 | -1.69E-46 |
| MS.gene052077 | MS.gene071407 | 0.86892 | 4.56E-66 | -1.69E-46 |
| MS.gene052078 | MS.gene071407 | 0.826626 | 2.45E-54 | -1.69E-46 |
| MS.gene052178 | MS.gene071407 | 0.841315 | 5.12E-58 | -1.69E-46 |
| MS.gene052188 | MS.gene071407 | 0.850268 | 1.89E-60 | -1.69E-46 |
| MS.gene052207 | MS.gene071407 | 0.818223 | 2.19E-52 | -1.69E-46 |
| MS.gene052253 | MS.gene071407 | 0.833262 | 5.90E-56 | -1.69E-46 |
| MS.gene052255 | MS.gene071407 | 0.813497 | 2.48E-51 | -1.69E-46 |
| MS.gene052309 | MS.gene071407 | -0.81761 | 3.02E-52 | -1.69E-46 |
| MS.gene052310 | MS.gene071407 | 0.828308 | 9.66E-55 | -1.69E-46 |
| MS.gene052340 | MS.gene071407 | 0.86999 | 2.05E-66 | -1.69E-46 |
| MS.gene053073 | MS.gene071407 | 0.821929 | 3.11E-53 | -1.69E-46 |
| MS.gene05316 | MS.gene071407 | 0.822672 | 2.09E-53 | -1.69E-46 |
| MS.gene053184 | MS.gene071407 | 0.810773 | 9.76E-51 | -1.69E-46 |
| MS.gene053209 | MS.gene071407 | 0.871243 | 7.93E-67 | -1.69E-46 |
| MS.gene053494 | MS.gene071407 | 0.809134 | 2.20E-50 | -1.69E-46 |
| MS.gene053557 | MS.gene071407 | 0.852177 | 5.48E-61 | -1.69E-46 |
| MS.gene053559 | MS.gene071407 | 0.822003 | 2.99E-53 | -1.69E-46 |
| MS.gene053577 | MS.gene071407 | 0.80504 | 1.62E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene071407 | 0.900553 | 6.53E-78 | -1.69E-46 |
| MS.gene053907 | MS.gene071407 | 0.805665 | 1.20E-49 | -1.69E-46 |
| MS.gene054123 | MS.gene071407 | 0.806129 | 9.55E-50 | -1.69E-46 |
| MS.gene054349 | MS.gene071407 | 0.806306 | 8.77E-50 | -1.69E-46 |
| MS.gene054379 | MS.gene071407 | 0.816734 | 4.75E-52 | -1.69E-46 |
| MS.gene054473 | MS.gene071407 | 0.842348 | 2.73E-58 | -1.69E-46 |
| MS.gene054542 | MS.gene071407 | 0.852638 | 4.05E-61 | -1.69E-46 |
| MS.gene054667 | MS.gene071407 | 0.808482 | 3.03E-50 | -1.69E-46 |
| MS.gene054930 | MS.gene071407 | 0.882624 | 8.91E-71 | -1.69E-46 |
| MS.gene054942 | MS.gene071407 | 0.812578 | 3.95E-51 | -1.69E-46 |
| MS.gene054983 | MS.gene071407 | 0.826749 | 2.29E-54 | -1.69E-46 |
| MS.gene055116 | MS.gene071407 | 0.851272 | 9.89E-61 | -1.69E-46 |
| MS.gene055277 | MS.gene071407 | 0.875116 | 3.97E-68 | -1.69E-46 |
| MS.gene055390 | MS.gene071407 | 0.829993 | 3.77E-55 | -1.69E-46 |
| MS.gene055422 | MS.gene071407 | 0.886199 | 4.21E-72 | -1.69E-46 |
| MS.gene055428 | MS.gene071407 | 0.85534 | 6.74E-62 | -1.69E-46 |
| MS.gene05553 | MS.gene071407 | 0.823553 | 1.30E-53 | -1.69E-46 |
| MS.gene055604 | MS.gene071407 | 0.804704 | 1.90E-49 | -1.69E-46 |
| MS.gene055712 | MS.gene071407 | 0.825019 | 5.89E-54 | -1.69E-46 |
| MS.gene055859 | MS.gene071407 | 0.838879 | 2.21E-57 | -1.69E-46 |
| MS.gene056069 | MS.gene071407 | -0.810199 | 1.30E-50 | -1.69E-46 |
| MS.gene056314 | MS.gene071407 | 0.81078 | 9.72E-51 | -1.69E-46 |
| MS.gene056407 | MS.gene071407 | 0.836375 | 9.71E-57 | -1.69E-46 |
| MS.gene056447 | MS.gene071407 | 0.805319 | 1.41E-49 | -1.69E-46 |
| MS.gene056609 | MS.gene071407 | 0.802824 | 4.67E-49 | -1.69E-46 |
| MS.gene056612 | MS.gene071407 | 0.854288 | 1.36E-61 | -1.69E-46 |
| MS.gene056659 | MS.gene071407 | 0.823592 | 1.28E-53 | -1.69E-46 |
| MS.gene056723 | MS.gene071407 | 0.811912 | 5.52E-51 | -1.69E-46 |
| MS.gene056875 | MS.gene071407 | 0.802487 | 5.48E-49 | -1.69E-46 |
| MS.gene056899 | MS.gene071407 | -0.808327 | 3.27E-50 | -1.69E-46 |
| MS.gene056979 | MS.gene071407 | 0.852802 | 3.63E-61 | -1.69E-46 |
| MS.gene05702 | MS.gene071407 | 0.856901 | 2.35E-62 | -1.69E-46 |
| MS.gene057121 | MS.gene071407 | 0.840705 | 7.40E-58 | -1.69E-46 |
| MS.gene057245 | MS.gene071407 | 0.830459 | 2.90E-55 | -1.69E-46 |
| MS.gene057260 | MS.gene071407 | 0.808288 | 3.33E-50 | -1.69E-46 |
| MS.gene057396 | MS.gene071407 | 0.805794 | 1.12E-49 | -1.69E-46 |
| MS.gene057439 | MS.gene071407 | 0.843218 | 1.60E-58 | -1.69E-46 |
| MS.gene057448 | MS.gene071407 | 0.871323 | 7.46E-67 | -1.69E-46 |
| MS.gene057449 | MS.gene071407 | 0.858676 | 7.00E-63 | -1.69E-46 |
| MS.gene057450 | MS.gene071407 | 0.877189 | 7.67E-69 | -1.69E-46 |
| MS.gene057469 | MS.gene071407 | 0.821477 | 3.95E-53 | -1.69E-46 |
| MS.gene057754 | MS.gene071407 | 0.822953 | 1.80E-53 | -1.69E-46 |
| MS.gene057796 | MS.gene071407 | 0.801417 | 9.09E-49 | -1.69E-46 |
| MS.gene058002 | MS.gene071407 | 0.874983 | 4.41E-68 | -1.69E-46 |
| MS.gene058082 | MS.gene071407 | 0.813262 | 2.80E-51 | -1.69E-46 |
| MS.gene05811 | MS.gene071407 | 0.862349 | 5.40E-64 | -1.69E-46 |
| MS.gene058142 | MS.gene071407 | 0.837245 | 5.82E-57 | -1.69E-46 |
| MS.gene058276 | MS.gene071407 | 0.822088 | 2.86E-53 | -1.69E-46 |
| MS.gene058293 | MS.gene071407 | 0.802882 | 4.54E-49 | -1.69E-46 |
| MS.gene058660 | MS.gene071407 | 0.820623 | 6.22E-53 | -1.69E-46 |
| MS.gene058710 | MS.gene071407 | 0.837846 | 4.08E-57 | -1.69E-46 |
| MS.gene058749 | MS.gene071407 | 0.816886 | 4.39E-52 | -1.69E-46 |
| MS.gene058833 | MS.gene071407 | 0.801406 | 9.14E-49 | -1.69E-46 |
| MS.gene058845 | MS.gene071407 | 0.854908 | 8.99E-62 | -1.69E-46 |
| MS.gene05886 | MS.gene071407 | 0.823749 | 1.17E-53 | -1.69E-46 |
| MS.gene058928 | MS.gene071407 | 0.815458 | 9.15E-52 | -1.69E-46 |
| MS.gene059033 | MS.gene071407 | -0.801304 | 9.59E-49 | -1.69E-46 |
| MS.gene05907 | MS.gene071407 | 0.852915 | 3.37E-61 | -1.69E-46 |
| MS.gene059285 | MS.gene071407 | 0.866757 | 2.26E-65 | -1.69E-46 |
| MS.gene059366 | MS.gene071407 | 0.811368 | 7.25E-51 | -1.69E-46 |
| MS.gene059420 | MS.gene071407 | 0.820933 | 5.28E-53 | -1.69E-46 |
| MS.gene059421 | MS.gene071407 | 0.804198 | 2.42E-49 | -1.69E-46 |
| MS.gene059518 | MS.gene071407 | 0.859059 | 5.37E-63 | -1.69E-46 |
| MS.gene059597 | MS.gene071407 | 0.85686 | 2.42E-62 | -1.69E-46 |
| MS.gene05959 | MS.gene071407 | 0.807302 | 5.40E-50 | -1.69E-46 |
| MS.gene059676 | MS.gene071407 | 0.828447 | 8.95E-55 | -1.69E-46 |
| MS.gene059688 | MS.gene071407 | 0.853234 | 2.73E-61 | -1.69E-46 |
| MS.gene059692 | MS.gene071407 | 0.863792 | 1.93E-64 | -1.69E-46 |
| MS.gene059695 | MS.gene071407 | 0.857048 | 2.13E-62 | -1.69E-46 |
| MS.gene05984 | MS.gene071407 | 0.88644 | 3.41E-72 | -1.69E-46 |
| MS.gene059894 | MS.gene071407 | 0.887595 | 1.24E-72 | -1.69E-46 |
| MS.gene059899 | MS.gene071407 | 0.86336 | 2.63E-64 | -1.69E-46 |
| MS.gene060084 | MS.gene071407 | 0.800894 | 1.16E-48 | -1.69E-46 |
| MS.gene06037 | MS.gene071407 | 0.853926 | 1.73E-61 | -1.69E-46 |
| MS.gene060407 | MS.gene071407 | 0.800502 | 1.40E-48 | -1.69E-46 |
| MS.gene060476 | MS.gene071407 | 0.829754 | 4.31E-55 | -1.69E-46 |
| MS.gene060495 | MS.gene071407 | 0.842617 | 2.32E-58 | -1.69E-46 |
| MS.gene060940 | MS.gene071407 | 0.818283 | 2.13E-52 | -1.69E-46 |
| MS.gene060942 | MS.gene071407 | 0.806919 | 6.51E-50 | -1.69E-46 |
| MS.gene060943 | MS.gene071407 | 0.815083 | 1.11E-51 | -1.69E-46 |
| MS.gene061002 | MS.gene071407 | 0.835065 | 2.08E-56 | -1.69E-46 |
| MS.gene061049 | MS.gene071407 | 0.895396 | 9.98E-76 | -1.69E-46 |
| MS.gene061051 | MS.gene071407 | -0.859423 | 4.18E-63 | -1.69E-46 |
| MS.gene061112 | MS.gene071407 | 0.807615 | 4.64E-50 | -1.69E-46 |
| MS.gene061114 | MS.gene071407 | -0.80483 | 1.79E-49 | -1.69E-46 |
| MS.gene061154 | MS.gene071407 | 0.813421 | 2.58E-51 | -1.69E-46 |
| MS.gene061159 | MS.gene071407 | 0.842443 | 2.58E-58 | -1.69E-46 |
| MS.gene061265 | MS.gene071407 | 0.808934 | 2.43E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071407.t1 | MTR_3g088160 | 98.750 | 160 | 2 | 0 | 1 | 160 | 277 | 436 | 1.65e-111 | 323 |
| MS.gene071407.t1 | MTR_3g088160 | 97.297 | 111 | 3 | 0 | 1 | 111 | 277 | 387 | 4.94e-74 | 226 |
| MS.gene071407.t1 | MTR_5g022510 | 48.980 | 98 | 37 | 2 | 12 | 98 | 151 | 246 | 3.51e-21 | 87.0 |
| MS.gene071407.t1 | MTR_5g022510 | 48.485 | 99 | 38 | 2 | 11 | 98 | 175 | 271 | 5.25e-21 | 87.0 |
| MS.gene071407.t1 | MTR_4g073410 | 54.762 | 84 | 29 | 2 | 2 | 85 | 174 | 248 | 7.26e-21 | 86.7 |
| MS.gene071407.t1 | MTR_4g061140 | 57.353 | 68 | 29 | 0 | 12 | 79 | 145 | 212 | 1.43e-19 | 81.6 |
| MS.gene071407.t1 | MTR_4g061140 | 57.353 | 68 | 29 | 0 | 12 | 79 | 179 | 246 | 2.60e-19 | 81.6 |
| MS.gene071407.t1 | MTR_4g061140 | 57.353 | 68 | 29 | 0 | 12 | 79 | 179 | 246 | 3.04e-19 | 81.6 |
| MS.gene071407.t1 | MTR_5g008440 | 50.000 | 68 | 34 | 0 | 12 | 79 | 114 | 181 | 2.49e-14 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071407.t1 | AT1G77490 | 77.987 | 159 | 31 | 1 | 1 | 159 | 262 | 416 | 6.16e-83 | 250 |
| MS.gene071407.t1 | AT1G77490 | 77.987 | 159 | 31 | 1 | 1 | 159 | 262 | 416 | 6.22e-83 | 250 |
| MS.gene071407.t1 | AT4G08390 | 84.444 | 90 | 14 | 0 | 1 | 90 | 282 | 371 | 2.60e-49 | 162 |
| MS.gene071407.t1 | AT4G08390 | 84.444 | 90 | 14 | 0 | 1 | 90 | 283 | 372 | 2.75e-49 | 162 |
| MS.gene071407.t1 | AT4G08390 | 84.444 | 90 | 14 | 0 | 1 | 90 | 283 | 372 | 2.75e-49 | 162 |
| MS.gene071407.t1 | AT4G08390 | 84.444 | 90 | 14 | 0 | 1 | 90 | 283 | 372 | 2.75e-49 | 162 |
| MS.gene071407.t1 | AT4G08390 | 61.111 | 90 | 9 | 1 | 1 | 90 | 283 | 346 | 8.15e-28 | 106 |
| MS.gene071407.t1 | AT3G09640 | 60.870 | 69 | 27 | 0 | 11 | 79 | 178 | 246 | 4.26e-21 | 86.7 |
| MS.gene071407.t1 | AT3G09640 | 60.870 | 69 | 27 | 0 | 11 | 79 | 178 | 246 | 4.26e-21 | 86.7 |
| MS.gene071407.t1 | AT4G35000 | 51.807 | 83 | 31 | 2 | 2 | 84 | 173 | 246 | 1.10e-19 | 83.6 |
| MS.gene071407.t1 | AT1G07890 | 57.971 | 69 | 29 | 0 | 11 | 79 | 178 | 246 | 1.19e-19 | 82.8 |
| MS.gene071407.t1 | AT1G07890 | 57.971 | 69 | 29 | 0 | 11 | 79 | 178 | 246 | 1.40e-19 | 82.4 |
| MS.gene071407.t1 | AT1G07890 | 57.971 | 69 | 29 | 0 | 11 | 79 | 178 | 246 | 1.40e-19 | 82.4 |
| MS.gene071407.t1 | AT1G07890 | 57.971 | 69 | 29 | 0 | 11 | 79 | 178 | 246 | 1.40e-19 | 82.4 |
| MS.gene071407.t1 | AT1G07890 | 57.971 | 69 | 29 | 0 | 11 | 79 | 178 | 246 | 1.40e-19 | 82.4 |
| MS.gene071407.t1 | AT1G07890 | 57.971 | 69 | 29 | 0 | 11 | 79 | 178 | 246 | 1.40e-19 | 82.4 |
| MS.gene071407.t1 | AT1G07890 | 57.971 | 69 | 29 | 0 | 11 | 79 | 178 | 246 | 1.40e-19 | 82.4 |
| MS.gene071407.t1 | AT1G07890 | 57.971 | 69 | 29 | 0 | 11 | 79 | 178 | 246 | 1.40e-19 | 82.4 |
| MS.gene071407.t1 | AT4G35970 | 49.351 | 77 | 37 | 1 | 12 | 88 | 174 | 248 | 3.95e-15 | 71.2 |
Find 36 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTCCAAGGTTGCTGAGTT+TGG | 0.256056 | 3.1:+71232612 | None:intergenic |
| CATAATTGTTATTGCTGCTT+TGG | 0.283340 | 3.1:-71231888 | MS.gene071407:CDS |
| TTTGACTTTAGAGCCTTATC+TGG | 0.287367 | 3.1:+71231926 | None:intergenic |
| CAATAACAATTATGATGTTT+AGG | 0.296597 | 3.1:+71231897 | None:intergenic |
| AACTTCTCTCCCACTACATT+TGG | 0.336185 | 3.1:+71232342 | None:intergenic |
| AAAGATGGGCCAGGAGCACC+TGG | 0.354863 | 3.1:-71233042 | MS.gene071407:CDS |
| TTTCAGGGCATTGTAATAGA+TGG | 0.363698 | 3.1:-71232369 | MS.gene071407:intron |
| AACCTTTCCAGAGGAGTACT+TGG | 0.382167 | 3.1:+71232311 | None:intergenic |
| GTTTGACAACTCTTACTTCA+AGG | 0.396786 | 3.1:-71232989 | MS.gene071407:intron |
| TTTCTCATTCTACAGAAAGA+TGG | 0.433341 | 3.1:-71233057 | None:intergenic |
| CTTTAACCATTGTGCAGTCC+AGG | 0.440549 | 3.1:+71233011 | None:intergenic |
| CAGGTTCAAACTTTGCTCCA+AGG | 0.468266 | 3.1:+71232599 | None:intergenic |
| CATGCCAAACTCAGCAACCT+TGG | 0.474570 | 3.1:-71232616 | MS.gene071407:CDS |
| ATGCTATGAGGAAGAAGATA+CGG | 0.479276 | 3.1:-71231976 | MS.gene071407:CDS |
| CAACGTGAACAGACCTCTGC+AGG | 0.481098 | 3.1:+71232580 | None:intergenic |
| TTGACTTTAGAGCCTTATCT+GGG | 0.486207 | 3.1:+71231927 | None:intergenic |
| TTCTCATTCTACAGAAAGAT+GGG | 0.495885 | 3.1:-71233056 | None:intergenic |
| TGAGAAATATGCTGTAGATC+AGG | 0.501966 | 3.1:-71232668 | MS.gene071407:CDS |
| GAAGATACGGGCTGAATATG+AGG | 0.502305 | 3.1:-71231963 | MS.gene071407:CDS |
| GCTGAATATGAGGCGGTTGG+CGG | 0.508251 | 3.1:-71231953 | MS.gene071407:CDS |
| GTAGCAGCCAAGTACTCCTC+TGG | 0.510718 | 3.1:-71232318 | MS.gene071407:CDS |
| AGATGGGCCAGGAGCACCTG+GGG | 0.518802 | 3.1:-71233040 | MS.gene071407:CDS |
| GGTTGGCGGAAGCCCAGATA+AGG | 0.547496 | 3.1:-71231939 | MS.gene071407:CDS |
| TGCTATGAGGAAGAAGATAC+GGG | 0.568469 | 3.1:-71231975 | MS.gene071407:CDS |
| AAGATGGGCCAGGAGCACCT+GGG | 0.570888 | 3.1:-71233041 | MS.gene071407:CDS |
| AAGAGCTGTCTGATGCTATG+AGG | 0.570930 | 3.1:-71231988 | MS.gene071407:CDS |
| AGATGGTTCTCCAAATGTAG+TGG | 0.571290 | 3.1:-71232352 | MS.gene071407:CDS |
| ATTCTACAGAAAGATGGGCC+AGG | 0.587158 | 3.1:-71233051 | None:intergenic |
| CGGGCTGAATATGAGGCGGT+TGG | 0.594896 | 3.1:-71231956 | MS.gene071407:CDS |
| AGCAAAGTTTGAACCTGCAG+AGG | 0.602470 | 3.1:-71232593 | MS.gene071407:intron |
| GATGGTTCTCCAAATGTAGT+GGG | 0.610180 | 3.1:-71232351 | MS.gene071407:CDS |
| GACAATCCTGGACTGCACAA+TGG | 0.620331 | 3.1:-71233017 | MS.gene071407:CDS |
| AGCCAAGTACTCCTCTGGAA+AGG | 0.627771 | 3.1:-71232313 | MS.gene071407:intron |
| CAAGCTTAAAACCTTTCCAG+AGG | 0.659132 | 3.1:+71232302 | None:intergenic |
| GATGGGCCAGGAGCACCTGG+GGG | 0.675083 | 3.1:-71233039 | MS.gene071407:CDS |
| GATACGGGCTGAATATGAGG+CGG | 0.710767 | 3.1:-71231960 | MS.gene071407:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAAAAAGAAAACTTTAAT+AGG | + | chr3.1:71232507-71232526 | None:intergenic | 10.0% |
| !!! | ATATTTCACAAATAATTTTA+TGG | + | chr3.1:71232201-71232220 | None:intergenic | 10.0% |
| !!! | TAATTTTATGTGAATAAATA+TGG | + | chr3.1:71232165-71232184 | None:intergenic | 10.0% |
| !!! | TATTTCACAAATAATTTTAT+GGG | + | chr3.1:71232200-71232219 | None:intergenic | 10.0% |
| !!! | AGATTTTTTGATTATTTTGT+TGG | - | chr3.1:71231937-71231956 | MS.gene071407:CDS | 15.0% |
| !!! | ATTATTATCACATGTAATTT+TGG | - | chr3.1:71232832-71232851 | MS.gene071407:CDS | 15.0% |
| !!! | GATTTTTTGATTATTTTGTT+GGG | - | chr3.1:71231938-71231957 | MS.gene071407:CDS | 15.0% |
| !! | AAAACTTTAATAGGTGATTT+GGG | + | chr3.1:71232498-71232517 | None:intergenic | 20.0% |
| !! | AAATCTTAAATGAAGAAGAA+AGG | - | chr3.1:71232855-71232874 | MS.gene071407:intron | 20.0% |
| !! | AAATTATTTGTGAAATATGC+AGG | - | chr3.1:71232203-71232222 | MS.gene071407:intron | 20.0% |
| !! | CAATAACAATTATGATGTTT+AGG | + | chr3.1:71233007-71233026 | None:intergenic | 20.0% |
| !! | TTGTAATTATTGTTACATAC+AGG | - | chr3.1:71232035-71232054 | MS.gene071407:intron | 20.0% |
| !!! | ATTTTATAACAGTATAGGAA+GGG | - | chr3.1:71231974-71231993 | MS.gene071407:CDS | 20.0% |
| !!! | TCTTTTTTATCTGTTTTTCA+GGG | - | chr3.1:71232517-71232536 | MS.gene071407:intron | 20.0% |
| !!! | TTCTTTTTTATCTGTTTTTC+AGG | - | chr3.1:71232516-71232535 | MS.gene071407:intron | 20.0% |
| ! | AAAAAAGGATGAAGATCTAT+TGG | - | chr3.1:71232068-71232087 | MS.gene071407:intron | 25.0% |
| ! | ATGTGAATAAATATGGACAT+GGG | + | chr3.1:71232158-71232177 | None:intergenic | 25.0% |
| ! | GAAAACTTTAATAGGTGATT+TGG | + | chr3.1:71232499-71232518 | None:intergenic | 25.0% |
| ! | TATGTGAATAAATATGGACA+TGG | + | chr3.1:71232159-71232178 | None:intergenic | 25.0% |
| !! | GATTTTATAACAGTATAGGA+AGG | - | chr3.1:71231973-71231992 | MS.gene071407:CDS | 25.0% |
| !!! | TGTTTTGTGCATTTTTAGAT+GGG | - | chr3.1:71232350-71232369 | MS.gene071407:CDS | 25.0% |
| !!! | TTTTGATTATTTTGTTGGGA+GGG | - | chr3.1:71231942-71231961 | MS.gene071407:CDS | 25.0% |
| !!! | TTTTTGATTATTTTGTTGGG+AGG | - | chr3.1:71231941-71231960 | MS.gene071407:CDS | 25.0% |
| ACAGGACATCAAAGAAAAAA+AGG | - | chr3.1:71232053-71232072 | MS.gene071407:intron | 30.0% | |
| ATGCAGACATGAAAAAGAAA+AGG | + | chr3.1:71232434-71232453 | None:intergenic | 30.0% | |
| ! | ATTCAGCAATTTTACCTTGA+AGG | + | chr3.1:71232130-71232149 | None:intergenic | 30.0% |
| ! | CATAATTGTTATTGCTGCTT+TGG | - | chr3.1:71233013-71233032 | MS.gene071407:CDS | 30.0% |
| ! | GGGTGATTTTATAACAGTAT+AGG | - | chr3.1:71231969-71231988 | MS.gene071407:CDS | 30.0% |
| ! | TTCATTTTCTTGATGCTTGT+TGG | - | chr3.1:71232644-71232663 | MS.gene071407:CDS | 30.0% |
| !! | AAGAAGCTATTTTCAAATCG+TGG | - | chr3.1:71232808-71232827 | MS.gene071407:CDS | 30.0% |
| !! | AGAAGCTATTTTCAAATCGT+GGG | - | chr3.1:71232809-71232828 | MS.gene071407:CDS | 30.0% |
| !! | CTGTTTTGTGCATTTTTAGA+TGG | - | chr3.1:71232349-71232368 | MS.gene071407:CDS | 30.0% |
| !! | TTTGATTATTTTGTTGGGAG+GGG | - | chr3.1:71231943-71231962 | MS.gene071407:CDS | 30.0% |
| !!! | GCTATTTTGACATATCTGTT+TGG | - | chr3.1:71233035-71233054 | MS.gene071407:CDS | 30.0% |
| ATGCTATGAGGAAGAAGATA+CGG | - | chr3.1:71232925-71232944 | MS.gene071407:intron | 35.0% | |
| GTTTGACAACTCTTACTTCA+AGG | - | chr3.1:71231912-71231931 | MS.gene071407:CDS | 35.0% | |
| TGAGAAATATGCTGTAGATC+AGG | - | chr3.1:71232233-71232252 | MS.gene071407:intron | 35.0% | |
| ! | AGCAATTTTACCTTGAAGGA+AGG | + | chr3.1:71232126-71232145 | None:intergenic | 35.0% |
| ! | GTTCATTGTTTCTCTATTCC+TGG | - | chr3.1:71232723-71232742 | MS.gene071407:intron | 35.0% |
| !! | TTGACTTTAGAGCCTTATCT+GGG | + | chr3.1:71232977-71232996 | None:intergenic | 35.0% |
| !! | TTTCAGGGCATTGTAATAGA+TGG | - | chr3.1:71232532-71232551 | MS.gene071407:intron | 35.0% |
| !! | TTTGACTTTAGAGCCTTATC+TGG | + | chr3.1:71232978-71232997 | None:intergenic | 35.0% |
| AACTTCTCTCCCACTACATT+TGG | + | chr3.1:71232562-71232581 | None:intergenic | 40.0% | |
| AGATGGTTCTCCAAATGTAG+TGG | - | chr3.1:71232549-71232568 | MS.gene071407:intron | 40.0% | |
| CAAGCTTAAAACCTTTCCAG+AGG | + | chr3.1:71232602-71232621 | None:intergenic | 40.0% | |
| GATGGTTCTCCAAATGTAGT+GGG | - | chr3.1:71232550-71232569 | MS.gene071407:intron | 40.0% | |
| TAACAGTATAGGAAGGGAAG+AGG | - | chr3.1:71231980-71231999 | MS.gene071407:CDS | 40.0% | |
| TCAAAAAGAGCAGCATCAGT+TGG | + | chr3.1:71232099-71232118 | None:intergenic | 40.0% | |
| TGCTATGAGGAAGAAGATAC+GGG | - | chr3.1:71232926-71232945 | MS.gene071407:intron | 40.0% | |
| ! | TTTTACTCCTGCATCAAACC+AGG | + | chr3.1:71232744-71232763 | None:intergenic | 40.0% |
| ! | TTTTGACGATCCTTCCTTCA+AGG | - | chr3.1:71232113-71232132 | MS.gene071407:intron | 40.0% |
| !! | ATTATTTTGTTGGGAGGGGA+GGG | - | chr3.1:71231947-71231966 | MS.gene071407:CDS | 40.0% |
| AAGAGCTGTCTGATGCTATG+AGG | - | chr3.1:71232913-71232932 | MS.gene071407:intron | 45.0% | |
| AGCAAAGTTTGAACCTGCAG+AGG | - | chr3.1:71232308-71232327 | MS.gene071407:intron | 45.0% | |
| CAGGTTCAAACTTTGCTCCA+AGG | + | chr3.1:71232305-71232324 | None:intergenic | 45.0% | |
| CTTTAACCATTGTGCAGTCC+AGG | + | chr3.1:71231893-71231912 | None:intergenic | 45.0% | |
| ! | CTCTATTCCTGGTTTGATGC+AGG | - | chr3.1:71232734-71232753 | MS.gene071407:intron | 45.0% |
| ! | GAAGATACGGGCTGAATATG+AGG | - | chr3.1:71232938-71232957 | MS.gene071407:intron | 45.0% |
| !! | AACCTTTCCAGAGGAGTACT+TGG | + | chr3.1:71232593-71232612 | None:intergenic | 45.0% |
| !! | GATTATTTTGTTGGGAGGGG+AGG | - | chr3.1:71231946-71231965 | MS.gene071407:CDS | 45.0% |
| !! | TTATTTTGTTGGGAGGGGAG+GGG | - | chr3.1:71231948-71231967 | MS.gene071407:CDS | 45.0% |
| !! | TATTATATATATTTAATGAA+AGG | - | chr3.1:71232672-71232691 | MS.gene071407:CDS | 5.0% |
| CATGCCAAACTCAGCAACCT+TGG | - | chr3.1:71232285-71232304 | MS.gene071407:intron | 50.0% | |
| GACAATCCTGGACTGCACAA+TGG | - | chr3.1:71231884-71231903 | MS.gene071407:CDS | 50.0% | |
| GTATAGGAAGGGAAGAGGAG+AGG | - | chr3.1:71231985-71232004 | MS.gene071407:CDS | 50.0% | |
| TGCTCCAAGGTTGCTGAGTT+TGG | + | chr3.1:71232292-71232311 | None:intergenic | 50.0% | |
| ! | AGCCAAGTACTCCTCTGGAA+AGG | - | chr3.1:71232588-71232607 | MS.gene071407:intron | 50.0% |
| ! | GATACGGGCTGAATATGAGG+CGG | - | chr3.1:71232941-71232960 | MS.gene071407:intron | 50.0% |
| ! | TATTTTGTTGGGAGGGGAGG+GGG | - | chr3.1:71231949-71231968 | MS.gene071407:CDS | 50.0% |
| CAACGTGAACAGACCTCTGC+AGG | + | chr3.1:71232324-71232343 | None:intergenic | 55.0% | |
| GCTGAATATGAGGCGGTTGG+CGG | - | chr3.1:71232948-71232967 | MS.gene071407:intron | 55.0% | |
| GTAGCAGCCAAGTACTCCTC+TGG | - | chr3.1:71232583-71232602 | MS.gene071407:intron | 55.0% | |
| CGGGCTGAATATGAGGCGGT+TGG | - | chr3.1:71232945-71232964 | MS.gene071407:intron | 60.0% | |
| GGTTGGCGGAAGCCCAGATA+AGG | - | chr3.1:71232962-71232981 | MS.gene071407:intron | 60.0% | |
| !! | AAAGATGGGCCAGGAGCACC+TGG | - | chr3.1:71231859-71231878 | MS.gene071407:CDS | 60.0% |
| !! | AAGATGGGCCAGGAGCACCT+GGG | - | chr3.1:71231860-71231879 | MS.gene071407:CDS | 60.0% |
| GAGCACCTGGGGGACAATCC+TGG | - | chr3.1:71231872-71231891 | MS.gene071407:CDS | 65.0% | |
| GATTGTCCCCCAGGTGCTCC+TGG | + | chr3.1:71231871-71231890 | None:intergenic | 65.0% | |
| GCAGTCCAGGATTGTCCCCC+AGG | + | chr3.1:71231880-71231899 | None:intergenic | 65.0% | |
| !! | AGATGGGCCAGGAGCACCTG+GGG | - | chr3.1:71231861-71231880 | MS.gene071407:CDS | 65.0% |
| !! | GATGGGCCAGGAGCACCTGG+GGG | - | chr3.1:71231862-71231881 | MS.gene071407:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 71231859 | 71233064 | 71231859 | ID=MS.gene071407 |
| chr3.1 | mRNA | 71231859 | 71233064 | 71231859 | ID=MS.gene071407.t1;Parent=MS.gene071407 |
| chr3.1 | exon | 71232990 | 71233064 | 71232990 | ID=MS.gene071407.t1.exon1;Parent=MS.gene071407.t1 |
| chr3.1 | CDS | 71232990 | 71233064 | 71232990 | ID=cds.MS.gene071407.t1;Parent=MS.gene071407.t1 |
| chr3.1 | exon | 71232789 | 71232866 | 71232789 | ID=MS.gene071407.t1.exon2;Parent=MS.gene071407.t1 |
| chr3.1 | CDS | 71232789 | 71232866 | 71232789 | ID=cds.MS.gene071407.t1;Parent=MS.gene071407.t1 |
| chr3.1 | exon | 71232594 | 71232698 | 71232594 | ID=MS.gene071407.t1.exon3;Parent=MS.gene071407.t1 |
| chr3.1 | CDS | 71232594 | 71232698 | 71232594 | ID=cds.MS.gene071407.t1;Parent=MS.gene071407.t1 |
| chr3.1 | exon | 71232314 | 71232385 | 71232314 | ID=MS.gene071407.t1.exon4;Parent=MS.gene071407.t1 |
| chr3.1 | CDS | 71232314 | 71232385 | 71232314 | ID=cds.MS.gene071407.t1;Parent=MS.gene071407.t1 |
| chr3.1 | exon | 71231859 | 71232011 | 71231859 | ID=MS.gene071407.t1.exon5;Parent=MS.gene071407.t1 |
| chr3.1 | CDS | 71231859 | 71232011 | 71231859 | ID=cds.MS.gene071407.t1;Parent=MS.gene071407.t1 |
| Gene Sequence |
| Protein sequence |
