AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene47809


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47809.t1 MTR_2g101770 99.048 105 1 0 1 105 47 151 9.40e-75 218
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47809.t1 AT1G79850 69.697 99 27 2 1 97 50 147 2.05e-44 141

Find 19 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAATACGTTTCTTGTATTT+AGG 0.176759 2.1:-3407103 None:intergenic
CCTGTTCCTGCTAGGAATTC+TGG 0.226525 2.1:+3407238 MS.gene47809:CDS
GGTAAGGCTGATAATTCTTC+TGG 0.342682 2.1:+3407259 MS.gene47809:CDS
CTAAATACAAGAAACGTATT+AGG 0.362043 2.1:+3407104 MS.gene47809:CDS
TTGCTGTTCCTGTTCCTGCT+AGG 0.444869 2.1:+3407230 MS.gene47809:CDS
CCAGAATTCCTAGCAGGAAC+AGG 0.463154 2.1:-3407238 None:intergenic
TAGGATGAAGAAGAAGTATC+AGG 0.470403 2.1:+3407123 MS.gene47809:CDS
CGTTTCTTGTATTTAGGATG+AGG 0.484431 2.1:-3407097 None:intergenic
AGAAGAACAGCCTCAGACTC+AGG 0.489039 2.1:+3407312 MS.gene47809:CDS
CCTCGACATTCCGCTTCAAT+CGG 0.508100 2.1:+3407285 MS.gene47809:CDS
TCCTGCTAGGAATTCTGGTA+AGG 0.524466 2.1:+3407243 MS.gene47809:CDS
TCTTCTTGCTCCGATTGAAG+CGG 0.531289 2.1:-3407295 None:intergenic
GCCTTACCAGAATTCCTAGC+AGG 0.562008 2.1:-3407244 None:intergenic
CAAAACTAAGCCTGAGTCTG+AGG 0.567568 2.1:-3407322 None:intergenic
AATCAAAGCTATGAAAACAA+TGG 0.574259 2.1:+3407012 None:intergenic
CAACGACAAAACCGTGTCTG+TGG 0.582090 2.1:+3407057 MS.gene47809:CDS
AAAGCTATGAAAACAATGGA+AGG 0.583695 2.1:+3407016 None:intergenic
ACGAGTTACTTCCACAGACA+CGG 0.603802 2.1:-3407068 None:intergenic
CCGATTGAAGCGGAATGTCG+AGG 0.647031 2.1:-3407285 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAGTTTTATTCTTACTAAT+AGG - chr2.1:3407208-3407227 None:intergenic 15.0%
! CTAAATACAAGAAACGTATT+AGG + chr2.1:3407104-3407123 MS.gene47809:CDS 25.0%
! CTAATACGTTTCTTGTATTT+AGG - chr2.1:3407106-3407125 None:intergenic 25.0%
! CCTGAAAACGTTTTCAAAGT+TGG + chr2.1:3407154-3407173 MS.gene47809:CDS 35.0%
! CGTTTCTTGTATTTAGGATG+AGG - chr2.1:3407100-3407119 None:intergenic 35.0%
!! CCAACTTTGAAAACGTTTTC+AGG - chr2.1:3407157-3407176 None:intergenic 35.0%
!! TAGGATGAAGAAGAAGTATC+AGG + chr2.1:3407123-3407142 MS.gene47809:CDS 35.0%
GGTAAGGCTGATAATTCTTC+TGG + chr2.1:3407259-3407278 MS.gene47809:CDS 40.0%
ACGAGTTACTTCCACAGACA+CGG - chr2.1:3407071-3407090 None:intergenic 45.0%
TCCTGCTAGGAATTCTGGTA+AGG + chr2.1:3407243-3407262 MS.gene47809:CDS 45.0%
TCTTCTTGCTCCGATTGAAG+CGG - chr2.1:3407298-3407317 None:intergenic 45.0%
AGAAGAACAGCCTCAGACTC+AGG + chr2.1:3407312-3407331 MS.gene47809:CDS 50.0%
CAACGACAAAACCGTGTCTG+TGG + chr2.1:3407057-3407076 MS.gene47809:CDS 50.0%
CCAGAATTCCTAGCAGGAAC+AGG - chr2.1:3407241-3407260 None:intergenic 50.0%
CCTCGACATTCCGCTTCAAT+CGG + chr2.1:3407285-3407304 MS.gene47809:CDS 50.0%
CCTGTTCCTGCTAGGAATTC+TGG + chr2.1:3407238-3407257 MS.gene47809:CDS 50.0%
GCCTTACCAGAATTCCTAGC+AGG - chr2.1:3407247-3407266 None:intergenic 50.0%
! TTGCTGTTCCTGTTCCTGCT+AGG + chr2.1:3407230-3407249 MS.gene47809:CDS 50.0%
!! AGACACGGTTTTGTCGTTGG+TGG - chr2.1:3407056-3407075 None:intergenic 50.0%
!! CACAGACACGGTTTTGTCGT+TGG - chr2.1:3407059-3407078 None:intergenic 50.0%
CCGATTGAAGCGGAATGTCG+AGG - chr2.1:3407288-3407307 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 3407022 3407339 3407022 ID=MS.gene47809
chr2.1 mRNA 3407022 3407339 3407022 ID=MS.gene47809.t1;Parent=MS.gene47809
chr2.1 exon 3407022 3407339 3407022 ID=MS.gene47809.t1.exon1;Parent=MS.gene47809.t1
chr2.1 CDS 3407022 3407339 3407022 ID=cds.MS.gene47809.t1;Parent=MS.gene47809.t1
Gene Sequence

>MS.gene47809

ATGAAAACAATGGAAGGAAAAGTTGTGTGTGCCACCAACGACAAAACCGTGTCTGTGGAAGTAACTCGTTTAGCACCTCATCCTAAATACAAGAAACGTATTAGGATGAAGAAGAAGTATCAGGCTCATGATCCTGAAAACGTTTTCAAAGTTGGTGATGTTGTTCAGCTTTGTAAAATTAGACCTATTAGTAAGAATAAAACTTTTCTTGCTGTTCCTGTTCCTGCTAGGAATTCTGGTAAGGCTGATAATTCTTCTGGTGACCTCGACATTCCGCTTCAATCGGAGCAAGAAGAACAGCCTCAGACTCAGGCTTAG

Protein sequence

>MS.gene47809.t1

MKTMEGKVVCATNDKTVSVEVTRLAPHPKYKKRIRMKKKYQAHDPENVFKVGDVVQLCKIRPISKNKTFLAVPVPARNSGKADNSSGDLDIPLQSEQEEQPQTQA