Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57121.t1 | XP_013450869.1 | 96.3 | 81 | 3 | 0 | 47 | 127 | 106 | 186 | 7.20E-32 | 146.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57121.t1 | I3SYC3 | 96.3 | 81 | 3 | 0 | 47 | 127 | 106 | 186 | 5.2e-32 | 146.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049260 | MS.gene57121 | 0.804967 | 1.67E-49 | -1.69E-46 |
| MS.gene049284 | MS.gene57121 | 0.81012 | 1.35E-50 | -1.69E-46 |
| MS.gene049297 | MS.gene57121 | 0.84526 | 4.54E-59 | -1.69E-46 |
| MS.gene049346 | MS.gene57121 | 0.842425 | 2.61E-58 | -1.69E-46 |
| MS.gene049351 | MS.gene57121 | 0.859465 | 4.06E-63 | -1.69E-46 |
| MS.gene049389 | MS.gene57121 | 0.820778 | 5.73E-53 | -1.69E-46 |
| MS.gene049491 | MS.gene57121 | 0.842741 | 2.15E-58 | -1.69E-46 |
| MS.gene049599 | MS.gene57121 | -0.832915 | 7.20E-56 | -1.69E-46 |
| MS.gene049661 | MS.gene57121 | 0.828099 | 1.09E-54 | -1.69E-46 |
| MS.gene049662 | MS.gene57121 | 0.833346 | 5.62E-56 | -1.69E-46 |
| MS.gene049758 | MS.gene57121 | 0.912934 | 1.10E-83 | -1.69E-46 |
| MS.gene049910 | MS.gene57121 | 0.859039 | 5.45E-63 | -1.69E-46 |
| MS.gene050076 | MS.gene57121 | 0.855784 | 5.00E-62 | -1.69E-46 |
| MS.gene050099 | MS.gene57121 | 0.844912 | 5.64E-59 | -1.69E-46 |
| MS.gene050128 | MS.gene57121 | 0.840346 | 9.19E-58 | -1.69E-46 |
| MS.gene050226 | MS.gene57121 | 0.802713 | 4.92E-49 | -1.69E-46 |
| MS.gene050227 | MS.gene57121 | 0.809035 | 2.31E-50 | -1.69E-46 |
| MS.gene050231 | MS.gene57121 | 0.804242 | 2.37E-49 | -1.69E-46 |
| MS.gene050301 | MS.gene57121 | 0.912971 | 1.06E-83 | -1.69E-46 |
| MS.gene050319 | MS.gene57121 | -0.815542 | 8.77E-52 | -1.69E-46 |
| MS.gene050330 | MS.gene57121 | -0.818469 | 1.93E-52 | -1.69E-46 |
| MS.gene050397 | MS.gene57121 | 0.856619 | 2.85E-62 | -1.69E-46 |
| MS.gene050432 | MS.gene57121 | 0.812277 | 4.60E-51 | -1.69E-46 |
| MS.gene050464 | MS.gene57121 | 0.825017 | 5.90E-54 | -1.69E-46 |
| MS.gene050516 | MS.gene57121 | 0.806299 | 8.80E-50 | -1.69E-46 |
| MS.gene050529 | MS.gene57121 | 0.838898 | 2.19E-57 | -1.69E-46 |
| MS.gene05053 | MS.gene57121 | 0.809604 | 1.74E-50 | -1.69E-46 |
| MS.gene050558 | MS.gene57121 | 0.84232 | 2.78E-58 | -1.69E-46 |
| MS.gene050623 | MS.gene57121 | -0.810674 | 1.03E-50 | -1.69E-46 |
| MS.gene050642 | MS.gene57121 | 0.836996 | 6.75E-57 | -1.69E-46 |
| MS.gene050762 | MS.gene57121 | 0.800336 | 1.51E-48 | -1.69E-46 |
| MS.gene050763 | MS.gene57121 | 0.80277 | 4.79E-49 | -1.69E-46 |
| MS.gene050770 | MS.gene57121 | 0.80077 | 1.23E-48 | -1.69E-46 |
| MS.gene05101 | MS.gene57121 | 0.878963 | 1.84E-69 | -1.69E-46 |
| MS.gene05119 | MS.gene57121 | -0.823788 | 1.15E-53 | -1.69E-46 |
| MS.gene051471 | MS.gene57121 | 0.889052 | 3.42E-73 | -1.69E-46 |
| MS.gene051582 | MS.gene57121 | 0.818783 | 1.64E-52 | -1.69E-46 |
| MS.gene051662 | MS.gene57121 | 0.875496 | 2.94E-68 | -1.69E-46 |
| MS.gene051663 | MS.gene57121 | 0.878014 | 3.95E-69 | -1.69E-46 |
| MS.gene051860 | MS.gene57121 | 0.859577 | 3.76E-63 | -1.69E-46 |
| MS.gene051877 | MS.gene57121 | 0.811741 | 6.02E-51 | -1.69E-46 |
| MS.gene051983 | MS.gene57121 | 0.828246 | 1.00E-54 | -1.69E-46 |
| MS.gene052077 | MS.gene57121 | 0.819756 | 9.83E-53 | -1.69E-46 |
| MS.gene052078 | MS.gene57121 | 0.870026 | 1.99E-66 | -1.69E-46 |
| MS.gene052178 | MS.gene57121 | 0.874291 | 7.58E-68 | -1.69E-46 |
| MS.gene052188 | MS.gene57121 | 0.844773 | 6.14E-59 | -1.69E-46 |
| MS.gene052207 | MS.gene57121 | 0.85532 | 6.83E-62 | -1.69E-46 |
| MS.gene052253 | MS.gene57121 | 0.833471 | 5.23E-56 | -1.69E-46 |
| MS.gene052256 | MS.gene57121 | 0.809423 | 1.91E-50 | -1.69E-46 |
| MS.gene052287 | MS.gene57121 | 0.803055 | 4.18E-49 | -1.69E-46 |
| MS.gene052290 | MS.gene57121 | 0.801187 | 1.01E-48 | -1.69E-46 |
| MS.gene052310 | MS.gene57121 | 0.865842 | 4.40E-65 | -1.69E-46 |
| MS.gene052326 | MS.gene57121 | 0.878041 | 3.87E-69 | -1.69E-46 |
| MS.gene052340 | MS.gene57121 | 0.846815 | 1.71E-59 | -1.69E-46 |
| MS.gene052485 | MS.gene57121 | 0.804255 | 2.36E-49 | -1.69E-46 |
| MS.gene053073 | MS.gene57121 | 0.814698 | 1.35E-51 | -1.69E-46 |
| MS.gene053209 | MS.gene57121 | 0.831074 | 2.05E-55 | -1.69E-46 |
| MS.gene053557 | MS.gene57121 | 0.853529 | 2.25E-61 | -1.69E-46 |
| MS.gene053836 | MS.gene57121 | 0.921678 | 2.63E-88 | -1.69E-46 |
| MS.gene053907 | MS.gene57121 | 0.855257 | 7.12E-62 | -1.69E-46 |
| MS.gene053928 | MS.gene57121 | 0.818754 | 1.66E-52 | -1.69E-46 |
| MS.gene053971 | MS.gene57121 | 0.831027 | 2.11E-55 | -1.69E-46 |
| MS.gene054010 | MS.gene57121 | 0.804346 | 2.26E-49 | -1.69E-46 |
| MS.gene054011 | MS.gene57121 | 0.847384 | 1.20E-59 | -1.69E-46 |
| MS.gene054012 | MS.gene57121 | 0.827658 | 1.39E-54 | -1.69E-46 |
| MS.gene054013 | MS.gene57121 | 0.826159 | 3.16E-54 | -1.69E-46 |
| MS.gene054123 | MS.gene57121 | 0.838877 | 2.22E-57 | -1.69E-46 |
| MS.gene054376 | MS.gene57121 | 0.813646 | 2.30E-51 | -1.69E-46 |
| MS.gene054379 | MS.gene57121 | 0.806189 | 9.28E-50 | -1.69E-46 |
| MS.gene054473 | MS.gene57121 | 0.881531 | 2.22E-70 | -1.69E-46 |
| MS.gene05455 | MS.gene57121 | 0.850865 | 1.29E-60 | -1.69E-46 |
| MS.gene054667 | MS.gene57121 | 0.816814 | 4.56E-52 | -1.69E-46 |
| MS.gene054802 | MS.gene57121 | 0.808055 | 3.74E-50 | -1.69E-46 |
| MS.gene054803 | MS.gene57121 | 0.809268 | 2.06E-50 | -1.69E-46 |
| MS.gene054804 | MS.gene57121 | 0.808685 | 2.74E-50 | -1.69E-46 |
| MS.gene054930 | MS.gene57121 | 0.833209 | 6.08E-56 | -1.69E-46 |
| MS.gene054942 | MS.gene57121 | 0.855708 | 5.26E-62 | -1.69E-46 |
| MS.gene054983 | MS.gene57121 | 0.843722 | 1.18E-58 | -1.69E-46 |
| MS.gene055116 | MS.gene57121 | 0.812017 | 5.24E-51 | -1.69E-46 |
| MS.gene055277 | MS.gene57121 | 0.913756 | 4.25E-84 | -1.69E-46 |
| MS.gene055303 | MS.gene57121 | 0.803543 | 3.32E-49 | -1.69E-46 |
| MS.gene055390 | MS.gene57121 | 0.883359 | 4.80E-71 | -1.69E-46 |
| MS.gene055412 | MS.gene57121 | 0.800278 | 1.55E-48 | -1.69E-46 |
| MS.gene055422 | MS.gene57121 | 0.917239 | 6.77E-86 | -1.69E-46 |
| MS.gene055428 | MS.gene57121 | 0.9116 | 5.06E-83 | -1.69E-46 |
| MS.gene055604 | MS.gene57121 | 0.846884 | 1.64E-59 | -1.69E-46 |
| MS.gene055712 | MS.gene57121 | 0.82726 | 1.73E-54 | -1.69E-46 |
| MS.gene056069 | MS.gene57121 | -0.825064 | 5.74E-54 | -1.69E-46 |
| MS.gene056191 | MS.gene57121 | 0.818113 | 2.32E-52 | -1.69E-46 |
| MS.gene056314 | MS.gene57121 | 0.856211 | 3.75E-62 | -1.69E-46 |
| MS.gene056407 | MS.gene57121 | 0.837304 | 5.63E-57 | -1.69E-46 |
| MS.gene056410 | MS.gene57121 | 0.814693 | 1.35E-51 | -1.69E-46 |
| MS.gene056451 | MS.gene57121 | 0.806617 | 7.54E-50 | -1.69E-46 |
| MS.gene056612 | MS.gene57121 | 0.892807 | 1.13E-74 | -1.69E-46 |
| MS.gene056772 | MS.gene57121 | 0.810262 | 1.26E-50 | -1.69E-46 |
| MS.gene056875 | MS.gene57121 | 0.819323 | 1.23E-52 | -1.69E-46 |
| MS.gene056979 | MS.gene57121 | 0.832212 | 1.08E-55 | -1.69E-46 |
| MS.gene05702 | MS.gene57121 | 0.867633 | 1.19E-65 | -1.69E-46 |
| MS.gene057121 | MS.gene57121 | 0.871932 | 4.69E-67 | -1.69E-46 |
| MS.gene057123 | MS.gene57121 | 0.838496 | 2.78E-57 | -1.69E-46 |
| MS.gene057245 | MS.gene57121 | 0.883143 | 5.76E-71 | -1.69E-46 |
| MS.gene057260 | MS.gene57121 | 0.802549 | 5.32E-49 | -1.69E-46 |
| MS.gene05733 | MS.gene57121 | 0.866413 | 2.90E-65 | -1.69E-46 |
| MS.gene057439 | MS.gene57121 | 0.863914 | 1.77E-64 | -1.69E-46 |
| MS.gene057448 | MS.gene57121 | 0.810221 | 1.28E-50 | -1.69E-46 |
| MS.gene057449 | MS.gene57121 | 0.801851 | 7.41E-49 | -1.69E-46 |
| MS.gene057450 | MS.gene57121 | 0.825918 | 3.61E-54 | -1.69E-46 |
| MS.gene057469 | MS.gene57121 | 0.828349 | 9.45E-55 | -1.69E-46 |
| MS.gene057754 | MS.gene57121 | 0.800377 | 1.48E-48 | -1.69E-46 |
| MS.gene058002 | MS.gene57121 | 0.877535 | 5.81E-69 | -1.69E-46 |
| MS.gene058082 | MS.gene57121 | 0.812281 | 4.59E-51 | -1.69E-46 |
| MS.gene05811 | MS.gene57121 | 0.898914 | 3.32E-77 | -1.69E-46 |
| MS.gene058142 | MS.gene57121 | 0.838378 | 2.98E-57 | -1.69E-46 |
| MS.gene058233 | MS.gene57121 | 0.845649 | 3.56E-59 | -1.69E-46 |
| MS.gene058276 | MS.gene57121 | 0.815932 | 7.18E-52 | -1.69E-46 |
| MS.gene058293 | MS.gene57121 | 0.842417 | 2.62E-58 | -1.69E-46 |
| MS.gene058365 | MS.gene57121 | 0.804435 | 2.16E-49 | -1.69E-46 |
| MS.gene058660 | MS.gene57121 | 0.887241 | 1.70E-72 | -1.69E-46 |
| MS.gene058710 | MS.gene57121 | 0.803799 | 2.93E-49 | -1.69E-46 |
| MS.gene058749 | MS.gene57121 | 0.827561 | 1.46E-54 | -1.69E-46 |
| MS.gene058787 | MS.gene57121 | 0.812326 | 4.48E-51 | -1.69E-46 |
| MS.gene058845 | MS.gene57121 | 0.85539 | 6.51E-62 | -1.69E-46 |
| MS.gene05886 | MS.gene57121 | 0.808434 | 3.10E-50 | -1.69E-46 |
| MS.gene059033 | MS.gene57121 | -0.827077 | 1.91E-54 | -1.69E-46 |
| MS.gene05907 | MS.gene57121 | 0.891914 | 2.57E-74 | -1.69E-46 |
| MS.gene059228 | MS.gene57121 | 0.839519 | 1.51E-57 | -1.69E-46 |
| MS.gene059294 | MS.gene57121 | 0.804489 | 2.11E-49 | -1.69E-46 |
| MS.gene059326 | MS.gene57121 | 0.819632 | 1.05E-52 | -1.69E-46 |
| MS.gene059342 | MS.gene57121 | 0.806603 | 7.59E-50 | -1.69E-46 |
| MS.gene059518 | MS.gene57121 | 0.878543 | 2.58E-69 | -1.69E-46 |
| MS.gene059597 | MS.gene57121 | 0.883659 | 3.72E-71 | -1.69E-46 |
| MS.gene05959 | MS.gene57121 | 0.82016 | 7.94E-53 | -1.69E-46 |
| MS.gene059676 | MS.gene57121 | 0.85057 | 1.56E-60 | -1.69E-46 |
| MS.gene059688 | MS.gene57121 | 0.842973 | 1.86E-58 | -1.69E-46 |
| MS.gene059692 | MS.gene57121 | 0.854556 | 1.14E-61 | -1.69E-46 |
| MS.gene059695 | MS.gene57121 | 0.847561 | 1.07E-59 | -1.69E-46 |
| MS.gene059708 | MS.gene57121 | 0.832576 | 8.74E-56 | -1.69E-46 |
| MS.gene05984 | MS.gene57121 | 0.884284 | 2.19E-71 | -1.69E-46 |
| MS.gene059894 | MS.gene57121 | 0.916751 | 1.22E-85 | -1.69E-46 |
| MS.gene059899 | MS.gene57121 | 0.821212 | 4.55E-53 | -1.69E-46 |
| MS.gene060005 | MS.gene57121 | 0.806393 | 8.41E-50 | -1.69E-46 |
| MS.gene06037 | MS.gene57121 | 0.88099 | 3.48E-70 | -1.69E-46 |
| MS.gene060407 | MS.gene57121 | 0.809706 | 1.66E-50 | -1.69E-46 |
| MS.gene060476 | MS.gene57121 | 0.851206 | 1.03E-60 | -1.69E-46 |
| MS.gene060559 | MS.gene57121 | 0.811582 | 6.51E-51 | -1.69E-46 |
| MS.gene06090 | MS.gene57121 | 0.824004 | 1.02E-53 | -1.69E-46 |
| MS.gene061049 | MS.gene57121 | 0.910002 | 3.05E-82 | -1.69E-46 |
| MS.gene061051 | MS.gene57121 | -0.816794 | 4.60E-52 | -1.69E-46 |
| MS.gene061112 | MS.gene57121 | 0.811105 | 8.27E-51 | -1.69E-46 |
| MS.gene061265 | MS.gene57121 | 0.811026 | 8.60E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57121.t1 | MTR_6g007887 | 96.296 | 81 | 3 | 0 | 47 | 127 | 106 | 186 | 2.83e-49 | 156 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57121.t1 | AT5G14910 | 67.901 | 81 | 26 | 0 | 47 | 127 | 98 | 178 | 9.01e-33 | 113 |
| MS.gene57121.t1 | AT5G14910 | 67.901 | 81 | 26 | 0 | 47 | 127 | 98 | 178 | 9.01e-33 | 113 |
Find 24 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCTTTGACCAAAGCTTTAG+AGG | 0.283689 | 6.2:+3930283 | MS.gene57121:CDS |
| TTTGAAGCCTAAACCTTGTA+TGG | 0.362693 | 6.2:-3931879 | None:intergenic |
| TGAGGGACTCGCAACTTTAG+AGG | 0.364918 | 6.2:+3930909 | MS.gene57121:CDS |
| TTACAATCTCTCCAGCCTCC+AGG | 0.397196 | 6.2:-3924396 | None:intergenic |
| TAGAGCTACCTCTAAAGCTT+TGG | 0.398332 | 6.2:-3930291 | None:intergenic |
| TTTGAAGGTTAATGTATTTG+AGG | 0.412702 | 6.2:+3930891 | MS.gene57121:CDS |
| TACAGGTGTGGCTTCAAGTC+TGG | 0.428978 | 6.2:+3931850 | MS.gene57121:CDS |
| TTGAAGGTTAATGTATTTGA+GGG | 0.430100 | 6.2:+3930892 | MS.gene57121:CDS |
| GTAGAAACCATACAAGGTTT+AGG | 0.441670 | 6.2:+3931872 | MS.gene57121:CDS |
| AGGTGCTATTGAAGCGTTCG+AGG | 0.479622 | 6.2:+3924472 | MS.gene57121:CDS |
| TACAATCTCTCCAGCCTCCA+GGG | 0.498185 | 6.2:-3924395 | None:intergenic |
| AAGCTTGGAGATGGCGTTCG+AGG | 0.498428 | 6.2:+3924452 | MS.gene57121:CDS |
| ACCTCTAAAGCTTTGGTCAA+AGG | 0.511214 | 6.2:-3930284 | None:intergenic |
| TTGAAGCCACACCTGTAGCT+TGG | 0.514796 | 6.2:-3931844 | None:intergenic |
| CAGCAATGAGAAAACAAGCT+TGG | 0.534413 | 6.2:+3924437 | MS.gene57121:CDS |
| TTTCAATGTTTGAAGGCAGA+GGG | 0.537717 | 6.2:+3930236 | MS.gene57121:intron |
| CAGACAACTATCCAAGCTAC+AGG | 0.538730 | 6.2:+3931833 | MS.gene57121:CDS |
| TGAGAAAACAAGCTTGGAGA+TGG | 0.545195 | 6.2:+3924443 | MS.gene57121:CDS |
| TAGAAACTGAGATGAAGAAA+TGG | 0.558576 | 6.2:+3924343 | None:intergenic |
| TCTAAAGCTTTGGTCAAAGG+TGG | 0.599903 | 6.2:-3930281 | None:intergenic |
| GGTGCTATTGAAGCGTTCGA+GGG | 0.611330 | 6.2:+3924473 | MS.gene57121:CDS |
| AAACTGAGATGAAGAAATGG+TGG | 0.618572 | 6.2:+3924346 | None:intergenic |
| AGTCTGGTAGAAACCATACA+AGG | 0.620327 | 6.2:+3931866 | MS.gene57121:CDS |
| AACTATCCAAGCTACAGGTG+TGG | 0.646935 | 6.2:+3931838 | MS.gene57121:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 3924354 | 3931955 | 3924354 | ID=MS.gene57121 |
| chr6.2 | mRNA | 3924354 | 3931955 | 3924354 | ID=MS.gene57121.t1;Parent=MS.gene57121 |
| chr6.2 | exon | 3924354 | 3924494 | 3924354 | ID=MS.gene57121.t1.exon1;Parent=MS.gene57121.t1 |
| chr6.2 | CDS | 3924354 | 3924494 | 3924354 | ID=cds.MS.gene57121.t1;Parent=MS.gene57121.t1 |
| chr6.2 | exon | 3930251 | 3930304 | 3930251 | ID=MS.gene57121.t1.exon2;Parent=MS.gene57121.t1 |
| chr6.2 | CDS | 3930251 | 3930304 | 3930251 | ID=cds.MS.gene57121.t1;Parent=MS.gene57121.t1 |
| chr6.2 | exon | 3930871 | 3930930 | 3930871 | ID=MS.gene57121.t1.exon3;Parent=MS.gene57121.t1 |
| chr6.2 | CDS | 3930871 | 3930930 | 3930871 | ID=cds.MS.gene57121.t1;Parent=MS.gene57121.t1 |
| chr6.2 | exon | 3931824 | 3931955 | 3931824 | ID=MS.gene57121.t1.exon4;Parent=MS.gene57121.t1 |
| chr6.2 | CDS | 3931824 | 3931955 | 3931824 | ID=cds.MS.gene57121.t1;Parent=MS.gene57121.t1 |
| Gene Sequence |
| Protein sequence |
