AlfalfaGEDB | MS.gene76503

M. sativa cultivar XinJiangDaYe / MS.gene76503

KEGG: K02961
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00366.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76503.t1	RHN76391.1	99	105	1	0	1	105	71	175	1.10E-49	205.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76503.t1	P17092	79.6	98	19	1	1	98	38	134	3.4e-36	152.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76503.t1	B7FMT1	99.0	105	1	0	1	105	47	151	7.7e-50	205.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049171	MS.gene76503	0.874063	9.05E-68	-1.69E-46
MS.gene049173	MS.gene76503	0.850715	1.42E-60	-1.69E-46
MS.gene049217	MS.gene76503	0.856079	4.10E-62	-1.69E-46
MS.gene049222	MS.gene76503	0.858608	7.33E-63	-1.69E-46
MS.gene049232	MS.gene76503	0.847047	1.48E-59	-1.69E-46
MS.gene049285	MS.gene76503	0.898029	7.92E-77	-1.69E-46
MS.gene049320	MS.gene76503	0.884278	2.20E-71	-1.69E-46
MS.gene04939	MS.gene76503	0.810066	1.39E-50	-1.69E-46
MS.gene049627	MS.gene76503	0.842501	2.49E-58	-1.69E-46
MS.gene049633	MS.gene76503	0.813352	2.67E-51	-1.69E-46
MS.gene05010	MS.gene76503	0.846266	2.42E-59	-1.69E-46
MS.gene050163	MS.gene76503	0.925503	1.68E-90	-1.69E-46
MS.gene050165	MS.gene76503	0.938798	3.71E-99	-1.69E-46
MS.gene050323	MS.gene76503	0.81521	1.04E-51	-1.69E-46
MS.gene050333	MS.gene76503	0.902498	9.12E-79	-1.69E-46
MS.gene050444	MS.gene76503	0.817827	2.69E-52	-1.69E-46
MS.gene050496	MS.gene76503	0.815562	8.68E-52	-1.69E-46
MS.gene050504	MS.gene76503	0.902539	8.74E-79	-1.69E-46
MS.gene050518	MS.gene76503	0.895114	1.30E-75	-1.69E-46
MS.gene050577	MS.gene76503	0.840398	8.91E-58	-1.69E-46
MS.gene050612	MS.gene76503	0.90093	4.47E-78	-1.69E-46
MS.gene050675	MS.gene76503	0.861462	1.01E-63	-1.69E-46
MS.gene050699	MS.gene76503	0.872629	2.75E-67	-1.69E-46
MS.gene050772	MS.gene76503	0.82692	2.08E-54	-1.69E-46
MS.gene050832	MS.gene76503	-0.827999	1.15E-54	-1.69E-46
MS.gene050833	MS.gene76503	-0.806929	6.48E-50	-1.69E-46
MS.gene050876	MS.gene76503	0.876197	1.69E-68	-1.69E-46
MS.gene05087	MS.gene76503	0.864812	9.28E-65	-1.69E-46
MS.gene050904	MS.gene76503	0.835133	2.00E-56	-1.69E-46
MS.gene050986	MS.gene76503	0.881342	2.60E-70	-1.69E-46
MS.gene05100	MS.gene76503	0.864583	1.10E-64	-1.69E-46
MS.gene051317	MS.gene76503	0.854374	1.28E-61	-1.69E-46
MS.gene051364	MS.gene76503	-0.808454	3.07E-50	-1.69E-46
MS.gene051366	MS.gene76503	-0.81913	1.36E-52	-1.69E-46
MS.gene051367	MS.gene76503	-0.804642	1.96E-49	-1.69E-46
MS.gene051551	MS.gene76503	0.843463	1.38E-58	-1.69E-46
MS.gene051579	MS.gene76503	-0.811303	7.49E-51	-1.69E-46
MS.gene051810	MS.gene76503	0.867543	1.27E-65	-1.69E-46
MS.gene051949	MS.gene76503	0.871362	7.24E-67	-1.69E-46
MS.gene052164	MS.gene76503	0.807294	5.42E-50	-1.69E-46
MS.gene052271	MS.gene76503	0.814656	1.38E-51	-1.69E-46
MS.gene052379	MS.gene76503	0.805714	1.17E-49	-1.69E-46
MS.gene052482	MS.gene76503	0.815395	9.45E-52	-1.69E-46
MS.gene052486	MS.gene76503	0.823536	1.31E-53	-1.69E-46
MS.gene052916	MS.gene76503	0.82885	7.15E-55	-1.69E-46
MS.gene053203	MS.gene76503	0.823356	1.45E-53	-1.69E-46
MS.gene053213	MS.gene76503	0.849888	2.42E-60	-1.69E-46
MS.gene053284	MS.gene76503	0.825079	5.70E-54	-1.69E-46
MS.gene053285	MS.gene76503	0.805945	1.04E-49	-1.69E-46
MS.gene053286	MS.gene76503	0.848912	4.53E-60	-1.69E-46
MS.gene053632	MS.gene76503	0.808102	3.65E-50	-1.69E-46
MS.gene054600	MS.gene76503	0.81984	9.40E-53	-1.69E-46
MS.gene054638	MS.gene76503	0.837113	6.29E-57	-1.69E-46
MS.gene054642	MS.gene76503	0.884875	1.32E-71	-1.69E-46
MS.gene05470	MS.gene76503	0.848795	4.88E-60	-1.69E-46
MS.gene054954	MS.gene76503	0.906284	1.75E-80	-1.69E-46
MS.gene055204	MS.gene76503	-0.845963	2.92E-59	-1.69E-46
MS.gene055362	MS.gene76503	0.886624	2.91E-72	-1.69E-46
MS.gene055502	MS.gene76503	0.869158	3.82E-66	-1.69E-46
MS.gene055577	MS.gene76503	0.842056	3.26E-58	-1.69E-46
MS.gene055634	MS.gene76503	0.863579	2.25E-64	-1.69E-46
MS.gene055636	MS.gene76503	0.813702	2.24E-51	-1.69E-46
MS.gene055638	MS.gene76503	0.842235	2.93E-58	-1.69E-46
MS.gene055748	MS.gene76503	0.846837	1.69E-59	-1.69E-46
MS.gene055750	MS.gene76503	0.815662	8.24E-52	-1.69E-46
MS.gene055751	MS.gene76503	0.860955	1.44E-63	-1.69E-46
MS.gene055776	MS.gene76503	0.874849	4.90E-68	-1.69E-46
MS.gene055849	MS.gene76503	0.868286	7.31E-66	-1.69E-46
MS.gene05589	MS.gene76503	0.816982	4.18E-52	-1.69E-46
MS.gene055956	MS.gene76503	-0.818021	2.44E-52	-1.69E-46
MS.gene056083	MS.gene76503	0.811978	5.34E-51	-1.69E-46
MS.gene056374	MS.gene76503	0.820233	7.64E-53	-1.69E-46
MS.gene056504	MS.gene76503	0.86136	1.08E-63	-1.69E-46
MS.gene056510	MS.gene76503	0.857656	1.41E-62	-1.69E-46
MS.gene056549	MS.gene76503	0.836389	9.63E-57	-1.69E-46
MS.gene056550	MS.gene76503	-0.817749	2.81E-52	-1.69E-46
MS.gene056657	MS.gene76503	0.885453	8.03E-72	-1.69E-46
MS.gene056692	MS.gene76503	0.865819	4.48E-65	-1.69E-46
MS.gene056761	MS.gene76503	-0.820796	5.68E-53	-1.69E-46
MS.gene056898	MS.gene76503	0.82168	3.55E-53	-1.69E-46
MS.gene056910	MS.gene76503	0.809758	1.62E-50	-1.69E-46
MS.gene057129	MS.gene76503	0.806797	6.91E-50	-1.69E-46
MS.gene057148	MS.gene76503	0.849204	3.76E-60	-1.69E-46
MS.gene057246	MS.gene76503	0.805073	1.59E-49	-1.69E-46
MS.gene057416	MS.gene76503	0.807958	3.92E-50	-1.69E-46
MS.gene057831	MS.gene76503	0.841514	4.54E-58	-1.69E-46
MS.gene057986	MS.gene76503	0.852126	5.66E-61	-1.69E-46
MS.gene058334	MS.gene76503	-0.820587	6.34E-53	-1.69E-46
MS.gene058335	MS.gene76503	0.832437	9.46E-56	-1.69E-46
MS.gene058419	MS.gene76503	0.881216	2.89E-70	-1.69E-46
MS.gene058544	MS.gene76503	0.833269	5.88E-56	-1.69E-46
MS.gene058719	MS.gene76503	0.841773	3.88E-58	-1.69E-46
MS.gene058723	MS.gene76503	0.810211	1.29E-50	-1.69E-46
MS.gene058773	MS.gene76503	0.873682	1.22E-67	-1.69E-46
MS.gene059022	MS.gene76503	0.903395	3.63E-79	-1.69E-46
MS.gene059023	MS.gene76503	0.839814	1.26E-57	-1.69E-46
MS.gene059026	MS.gene76503	0.816122	6.51E-52	-1.69E-46
MS.gene059029	MS.gene76503	0.830433	2.95E-55	-1.69E-46
MS.gene059079	MS.gene76503	0.853388	2.47E-61	-1.69E-46
MS.gene05912	MS.gene76503	0.874615	5.88E-68	-1.69E-46
MS.gene059479	MS.gene76503	-0.8181	2.34E-52	-1.69E-46
MS.gene059677	MS.gene76503	0.916769	1.20E-85	-1.69E-46
MS.gene059718	MS.gene76503	0.852731	3.81E-61	-1.69E-46
MS.gene059756	MS.gene76503	0.842124	3.13E-58	-1.69E-46
MS.gene059801	MS.gene76503	0.804518	2.08E-49	-1.69E-46
MS.gene059865	MS.gene76503	0.873155	1.83E-67	-1.69E-46
MS.gene060337	MS.gene76503	-0.861433	1.03E-63	-1.69E-46
MS.gene060703	MS.gene76503	0.906783	1.03E-80	-1.69E-46
MS.gene060762	MS.gene76503	0.878854	2.00E-69	-1.69E-46
MS.gene06077	MS.gene76503	0.860422	2.09E-63	-1.69E-46
MS.gene060823	MS.gene76503	-0.842506	2.48E-58	-1.69E-46
MS.gene060982	MS.gene76503	0.847657	1.01E-59	-1.69E-46
MS.gene061150	MS.gene76503	0.868578	5.88E-66	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76503.t1	MTR_2g101770	99.048	105	1	0	1	105	47	151	9.40e-75	218

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76503.t1	AT1G79850	69.697	99	27	2	1	97	50	147	2.05e-44	141

Find 19 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTAATACGTTTCTTGTATTT+AGG	0.176759	2.3:-2370787	None:intergenic
CCTGTTCCTGCTAGGAATTC+TGG	0.226525	2.3:+2370922	MS.gene76503:CDS
GGTAAGGCTGATAATTCTTC+TGG	0.342682	2.3:+2370943	MS.gene76503:CDS
CTAAATACAAGAAACGTATT+AGG	0.362043	2.3:+2370788	MS.gene76503:CDS
TTGCTGTTCCTGTTCCTGCT+AGG	0.444869	2.3:+2370914	MS.gene76503:CDS
CCAGAATTCCTAGCAGGAAC+AGG	0.463154	2.3:-2370922	None:intergenic
TAGGATGAAGAAGAAGTATC+AGG	0.470403	2.3:+2370807	MS.gene76503:CDS
CGTTTCTTGTATTTAGGATG+AGG	0.484431	2.3:-2370781	None:intergenic
AGAAGAACAGCCTCAGACTC+AGG	0.489039	2.3:+2370996	MS.gene76503:CDS
CCTCGACATTCCGCTTCAAT+CGG	0.508100	2.3:+2370969	MS.gene76503:CDS
TCCTGCTAGGAATTCTGGTA+AGG	0.524466	2.3:+2370927	MS.gene76503:CDS
TCTTCTTGCTCCGATTGAAG+CGG	0.531289	2.3:-2370979	None:intergenic
GCCTTACCAGAATTCCTAGC+AGG	0.562008	2.3:-2370928	None:intergenic
CAAAACTAAGCCTGAGTCTG+AGG	0.567568	2.3:-2371006	None:intergenic
AATCAAAGCTATGAAAACAA+TGG	0.574259	2.3:+2370696	None:intergenic
CAACGACAAAACCGTGTCTG+TGG	0.582090	2.3:+2370741	MS.gene76503:CDS
AAAGCTATGAAAACAATGGA+AGG	0.583695	2.3:+2370700	None:intergenic
ACGAGTTACTTCCACAGACA+CGG	0.603802	2.3:-2370752	None:intergenic
CCGATTGAAGCGGAATGTCG+AGG	0.647031	2.3:-2370969	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAAGTTTTATTCTTACTAAT+AGG	-	chr2.3:2370892-2370911	None:intergenic	15.0%
!	CTAAATACAAGAAACGTATT+AGG	+	chr2.3:2370788-2370807	MS.gene76503:CDS	25.0%
!	CTAATACGTTTCTTGTATTT+AGG	-	chr2.3:2370790-2370809	None:intergenic	25.0%
!	CCTGAAAACGTTTTCAAAGT+TGG	+	chr2.3:2370838-2370857	MS.gene76503:CDS	35.0%
!	CGTTTCTTGTATTTAGGATG+AGG	-	chr2.3:2370784-2370803	None:intergenic	35.0%
!!	CCAACTTTGAAAACGTTTTC+AGG	-	chr2.3:2370841-2370860	None:intergenic	35.0%
!!	TAGGATGAAGAAGAAGTATC+AGG	+	chr2.3:2370807-2370826	MS.gene76503:CDS	35.0%
	GGTAAGGCTGATAATTCTTC+TGG	+	chr2.3:2370943-2370962	MS.gene76503:CDS	40.0%
	ACGAGTTACTTCCACAGACA+CGG	-	chr2.3:2370755-2370774	None:intergenic	45.0%
	TCCTGCTAGGAATTCTGGTA+AGG	+	chr2.3:2370927-2370946	MS.gene76503:CDS	45.0%
	TCTTCTTGCTCCGATTGAAG+CGG	-	chr2.3:2370982-2371001	None:intergenic	45.0%
	AGAAGAACAGCCTCAGACTC+AGG	+	chr2.3:2370996-2371015	MS.gene76503:CDS	50.0%
	CAACGACAAAACCGTGTCTG+TGG	+	chr2.3:2370741-2370760	MS.gene76503:CDS	50.0%
	CCAGAATTCCTAGCAGGAAC+AGG	-	chr2.3:2370925-2370944	None:intergenic	50.0%
	CCTCGACATTCCGCTTCAAT+CGG	+	chr2.3:2370969-2370988	MS.gene76503:CDS	50.0%
	CCTGTTCCTGCTAGGAATTC+TGG	+	chr2.3:2370922-2370941	MS.gene76503:CDS	50.0%
	GCCTTACCAGAATTCCTAGC+AGG	-	chr2.3:2370931-2370950	None:intergenic	50.0%
!	TTGCTGTTCCTGTTCCTGCT+AGG	+	chr2.3:2370914-2370933	MS.gene76503:CDS	50.0%
!!	AGACACGGTTTTGTCGTTGG+TGG	-	chr2.3:2370740-2370759	None:intergenic	50.0%
!!	CACAGACACGGTTTTGTCGT+TGG	-	chr2.3:2370743-2370762	None:intergenic	50.0%
	CCGATTGAAGCGGAATGTCG+AGG	-	chr2.3:2370972-2370991	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	2370706	2371023	2370706	ID=MS.gene76503
chr2.3	mRNA	2370706	2371023	2370706	ID=MS.gene76503.t1;Parent=MS.gene76503
chr2.3	exon	2370706	2371023	2370706	ID=MS.gene76503.t1.exon1;Parent=MS.gene76503.t1
chr2.3	CDS	2370706	2371023	2370706	ID=cds.MS.gene76503.t1;Parent=MS.gene76503.t1

Gene Sequence	>MS.gene76503 ATGAAAACAATGGAAGGAAAAGTTGTGTGTGCCACCAACGACAAAACCGTGTCTGTGGAAGTAACTCGTTTAGCACCTCATCCTAAATACAAGAAACGTATTAGGATGAAGAAGAAGTATCAGGCTCATGATCCTGAAAACGTTTTCAAAGTTGGTGATGTTGTTCAGCTTTGTAAAATTAGACCTATTAGTAAGAATAAAACTTTTCTTGCTGTTCCTGTTCCTGCTAGGAATTCTGGTAAGGCTGATAATTCTTCTGGTGACCTCGACATTCCGCTTCAATCGGAGCAAGAAGAACAGCCTCAGACTCAGGCTTAG
Protein sequence	>MS.gene76503.t1 MKTMEGKVVCATNDKTVSVEVTRLAPHPKYKKRIRMKKKYQAHDPENVFKVGDVVQLCKIRPISKNKTFLAVPVPARNSGKADNSSGDLDIPLQSEQEEQPQTQA