AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89652


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89652.t1 MTR_8g075960 96.175 183 7 0 1 183 1 183 2.80e-129 360
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89652.t1 AT2G17972 47.794 136 57 3 46 181 37 158 1.15e-35 122

Find 53 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTCAATCTTCATAATGAT+TGG 0.317068 8.3:-27886210 MS.gene89652:CDS
CGCCGCAGCCTTAGTTCAAC+TGG 0.318125 8.3:+27886411 None:intergenic
GAGAGAAAGGTGCTCTCTTT+TGG 0.319102 8.3:-27886251 MS.gene89652:CDS
CTCATGTCCTCCCATTTCTT+AGG 0.342760 8.3:+27886306 None:intergenic
GGTGCTACTGCTGCTGCTGC+AGG 0.353627 8.3:+27886497 None:intergenic
ATGGATAGCGTTTAGGTTTG+TGG 0.358027 8.3:-27886184 MS.gene89652:CDS
TTGGGCTATGGATAGCGTTT+AGG 0.362432 8.3:-27886191 MS.gene89652:CDS
CAAAGGCTGCTCCTAAGAAA+TGG 0.371169 8.3:-27886317 MS.gene89652:CDS
TTAGGAGCAGCCTTTGCCTT+GGG 0.384532 8.3:+27886324 None:intergenic
TGGATAGCGTTTAGGTTTGT+GGG 0.391731 8.3:-27886183 MS.gene89652:CDS
ACGTCTAGTTAGAATAATAT+TGG 0.400280 8.3:+27886599 None:intergenic
ACTGCTGCTGCTGCAGGTGA+TGG 0.408197 8.3:+27886503 None:intergenic
AACTGGTTGTGTCTGGTTGA+TGG 0.417616 8.3:+27886564 None:intergenic
CTTCAATCTTCATAATGATT+GGG 0.425261 8.3:-27886209 MS.gene89652:CDS
CTTAGGAGCAGCCTTTGCCT+TGG 0.425440 8.3:+27886323 None:intergenic
TGCACTTAATATTTATCAGT+TGG 0.425443 8.3:-27886157 MS.gene89652:CDS
GCCAGAAAACAGAGAGAGAA+TGG 0.442782 8.3:-27886372 MS.gene89652:CDS
ACTGGTTGTGTCTGGTTGAT+GGG 0.451612 8.3:+27886565 None:intergenic
GAGCTTTATGTAGGAGAGAA+AGG 0.453819 8.3:-27886264 MS.gene89652:CDS
CTTAGTTCAACTGGTTCAGC+TGG 0.457887 8.3:+27886420 None:intergenic
TGAAGGTGAGTTTGAAGAGA+GGG 0.458101 8.3:+27886626 None:intergenic
TCAACTGGTTCAGCTGGTGT+TGG 0.466879 8.3:+27886426 None:intergenic
GTGAAGGTGAGTTTGAAGAG+AGG 0.477627 8.3:+27886625 None:intergenic
AAGGCACTTGAGCTTTATGT+AGG 0.483643 8.3:-27886273 MS.gene89652:CDS
GGTTCAGCTGGTGTTGGAGT+TGG 0.493855 8.3:+27886432 None:intergenic
ATCGTCGTTGGTGTTTGTGT+TGG 0.496861 8.3:+27886476 None:intergenic
TGAGTTTGAAGAGAGGGAGG+TGG 0.502944 8.3:+27886632 None:intergenic
TGAGAGGAACTGGTTGTGTC+TGG 0.505814 8.3:+27886557 None:intergenic
TAGGTTGAAATGAGAGGAAC+TGG 0.508744 8.3:+27886547 None:intergenic
CAGCTGAACCAGTTGAACTA+AGG 0.513049 8.3:-27886419 MS.gene89652:CDS
AAAGGCTGCTCCTAAGAAAT+GGG 0.520681 8.3:-27886316 MS.gene89652:CDS
TGCTGCTGCAGGTGATGGAT+TGG 0.523749 8.3:+27886508 None:intergenic
AGGTGAGTTTGAAGAGAGGG+AGG 0.534562 8.3:+27886629 None:intergenic
AACTGATAAATATTAAGTGC+AGG 0.538359 8.3:+27886159 None:intergenic
GATCCCTTCAGTACATCCTC+AGG 0.542081 8.3:+27886117 None:intergenic
TCTTCATAATGATTGGGCTA+TGG 0.548140 8.3:-27886203 MS.gene89652:CDS
GTTGTGTCTGGTTGATGGGA+GGG 0.554560 8.3:+27886569 None:intergenic
GTCACCTGAGGATGTACTGA+AGG 0.556978 8.3:-27886121 MS.gene89652:CDS
AAGTGGTAGGTTGAAATGAG+AGG 0.590851 8.3:+27886541 None:intergenic
AGAATAATATTGGTGAGTGA+AGG 0.591713 8.3:+27886609 None:intergenic
GGACATGAGTTTCACAGAGA+AGG 0.593588 8.3:-27886292 MS.gene89652:CDS
GGTTGTGTCTGGTTGATGGG+AGG 0.602056 8.3:+27886568 None:intergenic
CATACAGCAACCCAAGGCAA+AGG 0.612470 8.3:-27886334 MS.gene89652:CDS
TGGAAAGTGAGGGAAGTGGT+AGG 0.621474 8.3:+27886528 None:intergenic
AACCAGTTGAACTAAGGCTG+CGG 0.639608 8.3:-27886413 MS.gene89652:CDS
ACATCCTCAGGTGACAGTAG+AGG 0.640948 8.3:+27886129 None:intergenic
GGTGATGGATTGGAAAGTGA+GGG 0.642731 8.3:+27886518 None:intergenic
TCACCTGAGGATGTACTGAA+GGG 0.654909 8.3:-27886120 MS.gene89652:CDS
GGCTGCTCCTAAGAAATGGG+AGG 0.656833 8.3:-27886313 MS.gene89652:CDS
AAATAGCATACAGCAACCCA+AGG 0.666926 8.3:-27886340 MS.gene89652:CDS
AGGTGATGGATTGGAAAGTG+AGG 0.686558 8.3:+27886517 None:intergenic
GGATTGGAAAGTGAGGGAAG+TGG 0.688837 8.3:+27886524 None:intergenic
TCAACCTCTACTGTCACCTG+AGG 0.718719 8.3:-27886133 MS.gene89652:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AACTGATAAATATTAAGTGC+AGG + chr8.3:27886593-27886612 None:intergenic 25.0%
! ACGTCTAGTTAGAATAATAT+TGG + chr8.3:27886153-27886172 None:intergenic 25.0%
! CTTCAATCTTCATAATGATT+GGG - chr8.3:27886540-27886559 MS.gene89652:CDS 25.0%
! TGCACTTAATATTTATCAGT+TGG - chr8.3:27886592-27886611 MS.gene89652:CDS 25.0%
AGAATAATATTGGTGAGTGA+AGG + chr8.3:27886143-27886162 None:intergenic 30.0%
GCTTCAATCTTCATAATGAT+TGG - chr8.3:27886539-27886558 MS.gene89652:CDS 30.0%
TCTTCATAATGATTGGGCTA+TGG - chr8.3:27886546-27886565 MS.gene89652:CDS 35.0%
!! TGAATCTTTTTCATCGTCGT+TGG + chr8.3:27886288-27886307 None:intergenic 35.0%
AAAGGCTGCTCCTAAGAAAT+GGG - chr8.3:27886433-27886452 MS.gene89652:CDS 40.0%
AAATAGCATACAGCAACCCA+AGG - chr8.3:27886409-27886428 MS.gene89652:CDS 40.0%
TGAAGGTGAGTTTGAAGAGA+GGG + chr8.3:27886126-27886145 None:intergenic 40.0%
! AAGGCACTTGAGCTTTATGT+AGG - chr8.3:27886476-27886495 MS.gene89652:CDS 40.0%
! AAGTGGTAGGTTGAAATGAG+AGG + chr8.3:27886211-27886230 None:intergenic 40.0%
! ATGGATAGCGTTTAGGTTTG+TGG - chr8.3:27886565-27886584 MS.gene89652:CDS 40.0%
! GAGCTTTATGTAGGAGAGAA+AGG - chr8.3:27886485-27886504 MS.gene89652:CDS 40.0%
! TAGGTTGAAATGAGAGGAAC+TGG + chr8.3:27886205-27886224 None:intergenic 40.0%
! TCCATTCTCTCTCTGTTTTC+TGG + chr8.3:27886381-27886400 None:intergenic 40.0%
! TGGATAGCGTTTAGGTTTGT+GGG - chr8.3:27886566-27886585 MS.gene89652:CDS 40.0%
AACCAGTTGAACTAAGGCTG+CGG - chr8.3:27886336-27886355 MS.gene89652:CDS 45.0%
AACTGGTTGTGTCTGGTTGA+TGG + chr8.3:27886188-27886207 None:intergenic 45.0%
ACTGGTTGTGTCTGGTTGAT+GGG + chr8.3:27886187-27886206 None:intergenic 45.0%
CAAAGGCTGCTCCTAAGAAA+TGG - chr8.3:27886432-27886451 MS.gene89652:CDS 45.0%
CAGCTGAACCAGTTGAACTA+AGG - chr8.3:27886330-27886349 MS.gene89652:CDS 45.0%
CTCATGTCCTCCCATTTCTT+AGG + chr8.3:27886446-27886465 None:intergenic 45.0%
GCCAGAAAACAGAGAGAGAA+TGG - chr8.3:27886377-27886396 MS.gene89652:CDS 45.0%
GGACATGAGTTTCACAGAGA+AGG - chr8.3:27886457-27886476 MS.gene89652:CDS 45.0%
GTGAAGGTGAGTTTGAAGAG+AGG + chr8.3:27886127-27886146 None:intergenic 45.0%
TCACCTGAGGATGTACTGAA+GGG - chr8.3:27886629-27886648 MS.gene89652:CDS 45.0%
TTGGGCTATGGATAGCGTTT+AGG - chr8.3:27886558-27886577 MS.gene89652:CDS 45.0%
! ATCGTCGTTGGTGTTTGTGT+TGG + chr8.3:27886276-27886295 None:intergenic 45.0%
! CTTAGTTCAACTGGTTCAGC+TGG + chr8.3:27886332-27886351 None:intergenic 45.0%
!! AGGTGATGGATTGGAAAGTG+AGG + chr8.3:27886235-27886254 None:intergenic 45.0%
!! GGTGATGGATTGGAAAGTGA+GGG + chr8.3:27886234-27886253 None:intergenic 45.0%
!!! GAGAGAAAGGTGCTCTCTTT+TGG - chr8.3:27886498-27886517 MS.gene89652:CDS 45.0%
ACATCCTCAGGTGACAGTAG+AGG + chr8.3:27886623-27886642 None:intergenic 50.0%
AGGTGAGTTTGAAGAGAGGG+AGG + chr8.3:27886123-27886142 None:intergenic 50.0%
CATACAGCAACCCAAGGCAA+AGG - chr8.3:27886415-27886434 MS.gene89652:CDS 50.0%
GATCCCTTCAGTACATCCTC+AGG + chr8.3:27886635-27886654 None:intergenic 50.0%
GGATTGGAAAGTGAGGGAAG+TGG + chr8.3:27886228-27886247 None:intergenic 50.0%
GTCACCTGAGGATGTACTGA+AGG - chr8.3:27886628-27886647 MS.gene89652:CDS 50.0%
TCAACCTCTACTGTCACCTG+AGG - chr8.3:27886616-27886635 MS.gene89652:CDS 50.0%
TGAGAGGAACTGGTTGTGTC+TGG + chr8.3:27886195-27886214 None:intergenic 50.0%
TGAGTTTGAAGAGAGGGAGG+TGG + chr8.3:27886120-27886139 None:intergenic 50.0%
TGGAAAGTGAGGGAAGTGGT+AGG + chr8.3:27886224-27886243 None:intergenic 50.0%
TTAGGAGCAGCCTTTGCCTT+GGG + chr8.3:27886428-27886447 None:intergenic 50.0%
! TCAACTGGTTCAGCTGGTGT+TGG + chr8.3:27886326-27886345 None:intergenic 50.0%
!! GTTGTGTCTGGTTGATGGGA+GGG + chr8.3:27886183-27886202 None:intergenic 50.0%
CTTAGGAGCAGCCTTTGCCT+TGG + chr8.3:27886429-27886448 None:intergenic 55.0%
GGCTGCTCCTAAGAAATGGG+AGG - chr8.3:27886436-27886455 MS.gene89652:CDS 55.0%
! GGTTCAGCTGGTGTTGGAGT+TGG + chr8.3:27886320-27886339 None:intergenic 55.0%
!! GGTTGTGTCTGGTTGATGGG+AGG + chr8.3:27886184-27886203 None:intergenic 55.0%
!! TGCTGCTGCAGGTGATGGAT+TGG + chr8.3:27886244-27886263 None:intergenic 55.0%
ACTGCTGCTGCTGCAGGTGA+TGG + chr8.3:27886249-27886268 None:intergenic 60.0%
CGCCGCAGCCTTAGTTCAAC+TGG + chr8.3:27886341-27886360 None:intergenic 60.0%
!! GGTGCTACTGCTGCTGCTGC+AGG + chr8.3:27886255-27886274 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 27886110 27886661 27886110 ID=MS.gene89652
chr8.3 mRNA 27886110 27886661 27886110 ID=MS.gene89652.t1;Parent=MS.gene89652
chr8.3 exon 27886110 27886661 27886110 ID=MS.gene89652.t1.exon1;Parent=MS.gene89652.t1
chr8.3 CDS 27886110 27886661 27886110 ID=cds.MS.gene89652.t1;Parent=MS.gene89652.t1
Gene Sequence

>MS.gene89652

ATGGGAACCACCTCCCTCTCTTCAAACTCACCTTCACTCACCAATATTATTCTAACTAGACGTTCAAGTTCCCTCCCATCAACCAGACACAACCAGTTCCTCTCATTTCAACCTACCACTTCCCTCACTTTCCAATCCATCACCTGCAGCAGCAGCAGTAGCACCAACACAAACACCAACGACGATGAAAAAGATTCAGTTCAAGCACCAACTCCAACACCAGCTGAACCAGTTGAACTAAGGCTGCGGCGAAGATCAAAAAGACAAGCCAGAAAACAGAGAGAGAATGGAACTCTCACAAATAGCATACAGCAACCCAAGGCAAAGGCTGCTCCTAAGAAATGGGAGGACATGAGTTTCACAGAGAAGGCACTTGAGCTTTATGTAGGAGAGAAAGGTGCTCTCTTTTGGCATAACAAATTTGCATATGCTTCAATCTTCATAATGATTGGGCTATGGATAGCGTTTAGGTTTGTGGGTCCTGCACTTAATATTTATCAGTTGGATCAACCTCTACTGTCACCTGAGGATGTACTGAAGGGATCTTCCTGA

Protein sequence

>MS.gene89652.t1

MGTTSLSSNSPSLTNIILTRRSSSLPSTRHNQFLSFQPTTSLTFQSITCSSSSSTNTNTNDDEKDSVQAPTPTPAEPVELRLRRRSKRQARKQRENGTLTNSIQQPKAKAAPKKWEDMSFTEKALELYVGEKGALFWHNKFAYASIFIMIGLWIAFRFVGPALNIYQLDQPLLSPEDVLKGSS