AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene97396


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97396.t1 MTR_3g015440 89.412 85 9 0 1 85 143 227 2.95e-52 163
MS.gene97396.t1 MTR_3g015440 82.979 47 8 0 1 47 143 189 7.69e-23 87.8
MS.gene97396.t1 MTR_5g012030 43.373 83 45 1 1 83 28 108 4.58e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97396.t1 AT3G17670 58.824 85 35 0 1 85 136 220 1.51e-31 110
MS.gene97396.t1 AT3G17670 58.824 85 35 0 1 85 136 220 2.93e-31 110
MS.gene97396.t1 AT1G62740 42.169 83 46 1 1 83 28 108 1.46e-11 58.9
MS.gene97396.t1 AT1G12270 40.964 83 47 1 1 83 28 108 2.89e-11 57.8

Find 24 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTATAGATTTAAAACCATT+TGG 0.341600 3.1:-10101985 MS.gene97396:CDS
GGCATTGAACAAATTCGATT+TGG 0.354216 3.1:-10100643 MS.gene97396:CDS
CTGTCAAGGTGATGCCCATT+TGG 0.356146 3.1:-10100664 MS.gene97396:CDS
CGAATTTGTTCAATGCCAAA+TGG 0.393150 3.1:+10100649 None:intergenic
TTGGGTCACCTCTGAATACT+TGG 0.395476 3.1:+10101778 None:intergenic
CAAATTCGATTTGGCAGAGC+AGG 0.423742 3.1:-10100634 MS.gene97396:CDS
ACTGGAAATGGCAAACTACT+TGG 0.432811 3.1:-10101840 MS.gene97396:CDS
GTTTGCCATTTCCAGTCTTA+CGG 0.445012 3.1:+10101847 None:intergenic
GTCATCCGTAAGACTGGAAA+TGG 0.453951 3.1:-10101852 MS.gene97396:CDS
CCGTCAGTCTAAATCATTCA+AGG 0.480103 3.1:-10100577 None:intergenic
GAATTTGTTCAATGCCAAAT+GGG 0.511521 3.1:+10100650 None:intergenic
ATTCAGGTCATCCGTAAGAC+TGG 0.532385 3.1:-10101858 MS.gene97396:intron
AAGCATCCTTAGCATCTTCG+AGG 0.540528 3.1:+10101813 None:intergenic
ATAGATTTAAAACCATTTGG+AGG 0.543444 3.1:-10101982 MS.gene97396:CDS
GTGACATGTATACCTCCAAA+TGG 0.550224 3.1:+10101970 None:intergenic
GGGTGCCCTCGAAGATGCTA+AGG 0.560777 3.1:-10101819 MS.gene97396:CDS
TTGCAGGCTTATATCTGTCA+AGG 0.572022 3.1:-10100678 MS.gene97396:intron
CTGGAAATGGCAAACTACTT+GGG 0.572784 3.1:-10101839 MS.gene97396:CDS
TACATGTCACATATAAATGC+AGG 0.573446 3.1:-10101957 MS.gene97396:intron
TGGGTCACCTCTGAATACTT+GGG 0.585710 3.1:+10101779 None:intergenic
GATTTAGACTGACGGATCGA+AGG 0.592838 3.1:+10100585 None:intergenic
CCTTGAATGATTTAGACTGA+CGG 0.623941 3.1:+10100577 None:intergenic
AGCATCCTTAGCATCTTCGA+GGG 0.633887 3.1:+10101814 None:intergenic
ATTAGCTCCCAAGTATTCAG+AGG 0.657616 3.1:-10101786 MS.gene97396:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAATTATTATATGTATAT+TGG - chr3.1:10100645-10100664 MS.gene97396:CDS 10.0%
!! ATTTGTAATAACAAGTTAAA+TGG - chr3.1:10101069-10101088 MS.gene97396:intron 15.0%
!! AAGAAAAGAATATGAGAATT+TGG + chr3.1:10100807-10100826 None:intergenic 20.0%
!! ACAAAATTATAAGCTGATAT+TGG - chr3.1:10101322-10101341 MS.gene97396:intron 20.0%
!! AGAAAAGAATATGAGAATTT+GGG + chr3.1:10100806-10100825 None:intergenic 20.0%
!! AGCATTTGTAATTAATAAAG+TGG + chr3.1:10101026-10101045 None:intergenic 20.0%
!!! CTTTCTTATTGTTCTTATTT+CGG - chr3.1:10100681-10100700 MS.gene97396:intron 20.0%
!!! TTTCAAAACACTTGTTATAT+TGG + chr3.1:10101105-10101124 None:intergenic 20.0%
! AAATTTCAAGATTTGAACTC+CGG - chr3.1:10100929-10100948 MS.gene97396:intron 25.0%
! AACAAAGTTGACAAAAATAG+TGG - chr3.1:10101412-10101431 MS.gene97396:intron 25.0%
! ACTAGAAACTAATATGGATT+GGG - chr3.1:10101529-10101548 MS.gene97396:intron 25.0%
! ATATCAATATATGCAATGAC+CGG + chr3.1:10100951-10100970 None:intergenic 25.0%
! ATTCACTAACTACCTAAATA+TGG + chr3.1:10101642-10101661 None:intergenic 25.0%
! ATTGATGTTTGTTTAAATGC+AGG + chr3.1:10101801-10101820 None:intergenic 25.0%
! TATTTCTTTATTCACTTTGC+AGG - chr3.1:10101869-10101888 MS.gene97396:intron 25.0%
! TTCACTAACTACCTAAATAT+GGG + chr3.1:10101641-10101660 None:intergenic 25.0%
! TTGTAATTAATAAAGTGGCA+AGG + chr3.1:10101021-10101040 None:intergenic 25.0%
!! ATAAGAATTGATTTAGCAGT+CGG + chr3.1:10101240-10101259 None:intergenic 25.0%
!! ATAGATTTAAAACCATTTGG+AGG - chr3.1:10100581-10100600 MS.gene97396:CDS 25.0%
!! GCTATAGATTTAAAACCATT+TGG - chr3.1:10100578-10100597 MS.gene97396:CDS 25.0%
!!! GATAATAGGATATTGATTCT+AGG + chr3.1:10100838-10100857 None:intergenic 25.0%
!!! GTTTTTTTTTGATCCTTTTG+AGG - chr3.1:10101139-10101158 MS.gene97396:intron 25.0%
!!! TAGTTTTTAGCAAGGATAAT+AGG + chr3.1:10100852-10100871 None:intergenic 25.0%
CACTAGAAACTAATATGGAT+TGG - chr3.1:10101528-10101547 MS.gene97396:intron 30.0%
CTTCAAATACTTATACGGAA+AGG + chr3.1:10101169-10101188 None:intergenic 30.0%
TACATGTCACATATAAATGC+AGG - chr3.1:10100606-10100625 MS.gene97396:CDS 30.0%
TTGAGTTAGCATTAAGAACA+TGG + chr3.1:10101602-10101621 None:intergenic 30.0%
! AATGTCACAACAAGAGATTT+TGG - chr3.1:10101699-10101718 MS.gene97396:intron 30.0%
! GAATTTGTTCAATGCCAAAT+GGG + chr3.1:10101916-10101935 None:intergenic 30.0%
! TCTTGAAATTTCTAGACTCT+AGG + chr3.1:10100920-10100939 None:intergenic 30.0%
!! CAGAATCTTGAGTTATTTTG+AGG + chr3.1:10101355-10101374 None:intergenic 30.0%
!!! GTTACTAGTAGTTTTTAGCA+AGG + chr3.1:10100860-10100879 None:intergenic 30.0%
AAATAGTGGCTACGAAGTTT+AGG - chr3.1:10101426-10101445 MS.gene97396:intron 35.0%
AACATACGATCTACAGGTAT+CGG - chr3.1:10101571-10101590 MS.gene97396:intron 35.0%
AATGATCATACCCACATTGA+TGG + chr3.1:10101758-10101777 None:intergenic 35.0%
ACTGAGATTCAAATCGTGAT+TGG - chr3.1:10101201-10101220 MS.gene97396:intron 35.0%
ATGCGCACTAGAAACTAATA+TGG - chr3.1:10101523-10101542 MS.gene97396:intron 35.0%
CCGTGCTTCAAATACTTATA+CGG + chr3.1:10101174-10101193 None:intergenic 35.0%
CTAATTGTTGCCAAAATCCA+TGG - chr3.1:10101672-10101691 MS.gene97396:intron 35.0%
GTCACATATAAATGCAGGTA+CGG - chr3.1:10100611-10100630 MS.gene97396:CDS 35.0%
TAAATATGGGAAACACGCAT+GGG + chr3.1:10101628-10101647 None:intergenic 35.0%
TAAGCTTAGCTAAACTGCTA+AGG + chr3.1:10100986-10101005 None:intergenic 35.0%
TGAATCAACATACGATCTAC+AGG - chr3.1:10101565-10101584 MS.gene97396:intron 35.0%
! CCGTATAAGTATTTGAAGCA+CGG - chr3.1:10101171-10101190 MS.gene97396:intron 35.0%
! CGAATTTGTTCAATGCCAAA+TGG + chr3.1:10101917-10101936 None:intergenic 35.0%
! GGCATTGAACAAATTCGATT+TGG - chr3.1:10101920-10101939 MS.gene97396:intron 35.0%
! TTGTTCTTATTTCGGCATTC+AGG - chr3.1:10100689-10100708 MS.gene97396:intron 35.0%
!! ACAACAAGAGATTTTGGTCT+TGG - chr3.1:10101705-10101724 MS.gene97396:intron 35.0%
!! TTTCTAGTGCGCATCTATTT+TGG + chr3.1:10101517-10101536 None:intergenic 35.0%
ACTGGAAATGGCAAACTACT+TGG - chr3.1:10100723-10100742 MS.gene97396:intron 40.0%
ATTAGCTCCCAAGTATTCAG+AGG - chr3.1:10100777-10100796 MS.gene97396:intron 40.0%
CATGCGTGTTTCCCATATTT+AGG - chr3.1:10101627-10101646 MS.gene97396:intron 40.0%
CCGTCAGTCTAAATCATTCA+AGG - chr3.1:10101986-10102005 MS.gene97396:CDS 40.0%
CTAAATATGGGAAACACGCA+TGG + chr3.1:10101629-10101648 None:intergenic 40.0%
CTGGAAATGGCAAACTACTT+GGG - chr3.1:10100724-10100743 MS.gene97396:intron 40.0%
GTGACATGTATACCTCCAAA+TGG + chr3.1:10100596-10100615 None:intergenic 40.0%
TCTAGACTCTAGGCTAAAAG+TGG + chr3.1:10100910-10100929 None:intergenic 40.0%
TTGCAGGCTTATATCTGTCA+AGG - chr3.1:10101885-10101904 MS.gene97396:intron 40.0%
TTGTTGTGACATTGTAGCCA+TGG + chr3.1:10101692-10101711 None:intergenic 40.0%
! ATCATACCCACATTGATGGT+AGG + chr3.1:10101754-10101773 None:intergenic 40.0%
! GACATTGTAGCCATGGATTT+TGG + chr3.1:10101685-10101704 None:intergenic 40.0%
! GTTTGCCATTTCCAGTCTTA+CGG + chr3.1:10100719-10100738 None:intergenic 40.0%
AAGCATCCTTAGCATCTTCG+AGG + chr3.1:10100753-10100772 None:intergenic 45.0%
ACGGAAAGGTGAACCTCAAA+AGG + chr3.1:10101155-10101174 None:intergenic 45.0%
ACTCTAGGCTAAAAGTGGCA+AGG + chr3.1:10100905-10100924 None:intergenic 45.0%
AGCATCCTTAGCATCTTCGA+GGG + chr3.1:10100752-10100771 None:intergenic 45.0%
ATTCAGGTCATCCGTAAGAC+TGG - chr3.1:10100705-10100724 MS.gene97396:intron 45.0%
GCAGTTTAGCTAAGCTTACG+AGG - chr3.1:10100988-10101007 MS.gene97396:intron 45.0%
GTCATCCGTAAGACTGGAAA+TGG - chr3.1:10100711-10100730 MS.gene97396:intron 45.0%
TGCAGGCACAACAAATACAC+AGG + chr3.1:10101784-10101803 None:intergenic 45.0%
TGCCTACCTACCATCAATGT+GGG - chr3.1:10101745-10101764 MS.gene97396:intron 45.0%
TGGGTCACCTCTGAATACTT+GGG + chr3.1:10100787-10100806 None:intergenic 45.0%
TTGCCTACCTACCATCAATG+TGG - chr3.1:10101744-10101763 MS.gene97396:intron 45.0%
TTGGGTCACCTCTGAATACT+TGG + chr3.1:10100788-10100807 None:intergenic 45.0%
! GATTTAGACTGACGGATCGA+AGG + chr3.1:10101981-10102000 None:intergenic 45.0%
! TACCCACATTGATGGTAGGT+AGG + chr3.1:10101750-10101769 None:intergenic 45.0%
!! CAAATTCGATTTGGCAGAGC+AGG - chr3.1:10101929-10101948 MS.gene97396:intron 45.0%
!! AATAAATTAATAATGATTTA+CGG - chr3.1:10101830-10101849 MS.gene97396:CDS 5.0%
CTGTCAAGGTGATGCCCATT+TGG - chr3.1:10101899-10101918 MS.gene97396:intron 50.0%
GGGTGCCCTCGAAGATGCTA+AGG - chr3.1:10100744-10100763 MS.gene97396:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 10100578 10102007 10100578 ID=MS.gene97396
chr3.1 mRNA 10100578 10102007 10100578 ID=MS.gene97396.t1;Parent=MS.gene97396
chr3.1 exon 10101958 10102007 10101958 ID=MS.gene97396.t1.exon1;Parent=MS.gene97396.t1
chr3.1 CDS 10101958 10102007 10101958 ID=cds.MS.gene97396.t1;Parent=MS.gene97396.t1
chr3.1 exon 10101787 10101874 10101787 ID=MS.gene97396.t1.exon2;Parent=MS.gene97396.t1
chr3.1 CDS 10101787 10101874 10101787 ID=cds.MS.gene97396.t1;Parent=MS.gene97396.t1
chr3.1 exon 10100578 10100694 10100578 ID=MS.gene97396.t1.exon3;Parent=MS.gene97396.t1
chr3.1 CDS 10100578 10100694 10100578 ID=cds.MS.gene97396.t1;Parent=MS.gene97396.t1
Gene Sequence

>MS.gene97396

GCTATAGATTTAAAACCATTTGGAGGTATACATGTCACATATAAATGCAGGTACGGTTTGCACAAATCAAAATTATTATATGTATATTGGTGTTATCGTTTGTCTTTCTTATTGTTCTTATTTCGGCATTCAGGTCATCCGTAAGACTGGAAATGGCAAACTACTTGGGTGCCCTCGAAGATGCTAAGGATGCTTTGATATTAGCTCCCAAGTATTCAGAGGTGACCCAAATTCTCATATTCTTTTCTTATCAACTACCTAGAATCAATATCCTATTATCCTTGCTAAAAACTACTAGTAACTCATTCATTCTCATTTTAAAAACCTTGCCACTTTTAGCCTAGAGTCTAGAAATTTCAAGATTTGAACTCCGGTCATTGCATATATTGATATATAATGCAATGTCCTTAGCAGTTTAGCTAAGCTTACGAGGATAAAAACCTTGCCACTTTATTAATTACAAATGCTAAAGTTACACAACTAAACTTTTAATTTGTAATAACAAGTTAAATGGTTGAGAATTTCCAATATAACAAGTGTTTTGAAAATGCAATGTTGTGTGTTTTTTTTTGATCCTTTTGAGGTTCACCTTTCCGTATAAGTATTTGAAGCACGGTTATCAAACTGAGATTCAAATCGTGATTGGAAAGACGTATTTTCCGACTGCTAAATCAATTCTTATGATGAATAGTAAGATACTTATTAATAAAAATATGACATTTTTAAGTAAAAATAAGTTACATAACAAAATTATAAGCTGATATTGGATACAAACCTCAAAATAACTCAAGATTCTGATCATAAATCAAATGACAAAAGAAAAGTTGTTGACTAAACAAAGTTGACAAAAATAGTGGCTACGAAGTTTAGGTAGTGAATTATTAGAATGACTCGTGATTTAACACAGTGAATCATGACTTATGATAAGATTCATGTCCAAAATAGATGCGCACTAGAAACTAATATGGATTGGGTTCAATTATGTAGTGAATCAACATACGATCTACAGGTATCGGAAGAACCATGTTCTTAATGCTAACTCAATCTCCCATGCGTGTTTCCCATATTTAGGTAGTTAGTGAATGTCATACTTTCTAATTGTTGCCAAAATCCATGGCTACAATGTCACAACAAGAGATTTTGGTCTTGGTCTTGCTCGATTTAATTTGCCTACCTACCATCAATGTGGGTATGATCATTTTTCCTGTGTATTTGTTGTGCCTGCATTTAAACAAACATCAATTCAACGAAAAATAAATTAATAATGATTTACGGCTTCTCATCACTCTAATATTTCTTTATTCACTTTGCAGGCTTATATCTGTCAAGGTGATGCCCATTTGGCATTGAACAAATTCGATTTGGCAGAGCAGGCATATTTAGCATGTTTAGATATAGATCCTTCGATCCGTCAGTCTAAATCATTCAAG

Protein sequence

>MS.gene97396.t1

AIDLKPFGGIHVTYKCRSSVRLEMANYLGALEDAKDALILAPKYSEAYICQGDAHLALNKFDLAEQAYLACLDIDPSIRQSKSFK