AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048294.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048294.01.T01 MTR_4g055820 88.846 260 29 0 1 260 51 310 1.13e-172 480
MsG0080048294.01.T01 MTR_8g041270 63.498 263 90 2 4 260 51 313 6.40e-114 333
MsG0080048294.01.T01 MTR_2g104340 63.035 257 92 2 4 260 50 303 1.60e-109 322
MsG0080048294.01.T01 MTR_2g104280 58.555 263 103 2 4 260 50 312 1.11e-101 302
MsG0080048294.01.T01 MTR_2g104260 60.088 228 85 2 4 225 50 277 1.15e-92 275
MsG0080048294.01.T01 MTR_5g074800 50.193 259 119 5 4 260 2 252 3.08e-83 253
MsG0080048294.01.T01 MTR_7g069550 49.813 267 117 7 1 260 51 307 6.57e-78 241
MsG0080048294.01.T01 MTR_5g072060 47.510 261 112 4 1 260 43 279 1.09e-72 233
MsG0080048294.01.T01 MTR_2g021770 41.971 274 137 6 1 260 49 314 1.27e-64 207
MsG0080048294.01.T01 MTR_2g022000 41.516 277 140 7 1 260 53 324 6.61e-64 206
MsG0080048294.01.T01 MTR_2g021800 41.727 278 139 6 1 260 53 325 4.38e-63 203
MsG0080048294.01.T01 MTR_4g102410 43.704 270 130 8 1 260 98 355 9.68e-63 203
MsG0080048294.01.T01 MTR_5g096630 43.893 262 129 6 7 260 61 312 1.46e-61 199
MsG0080048294.01.T01 MTR_8g013000 46.591 264 128 8 1 260 47 301 5.04e-59 192
MsG0080048294.01.T01 MTR_8g063440 42.529 261 130 7 1 260 49 290 3.02e-58 190
MsG0080048294.01.T01 MTR_8g464770 43.130 262 122 9 2 260 50 287 3.80e-58 189
MsG0080048294.01.T01 MTR_5g097330 42.647 272 127 9 1 259 53 308 5.39e-55 182
MsG0080048294.01.T01 MTR_7g017130 40.741 270 130 8 1 260 55 304 3.52e-53 177
MsG0080048294.01.T01 MTR_7g017170 40.741 270 130 8 1 260 55 304 3.78e-53 177
MsG0080048294.01.T01 MTR_7g079640 43.396 265 112 9 1 260 50 281 7.41e-53 176
MsG0080048294.01.T01 MTR_0129s0070 40.293 273 127 10 1 260 54 303 2.92e-52 175
MsG0080048294.01.T01 MTR_2g021950 41.264 269 134 8 1 260 53 306 4.95e-52 174
MsG0080048294.01.T01 MTR_5g022850 42.105 228 119 4 37 260 41 259 7.12e-52 172
MsG0080048294.01.T01 MTR_4g117550 42.975 242 123 6 1 237 54 285 8.65e-52 173
MsG0080048294.01.T01 MTR_3g011120 41.353 266 121 10 1 260 51 287 9.23e-52 173
MsG0080048294.01.T01 MTR_2g064950 41.353 266 134 9 1 260 47 296 1.97e-51 172
MsG0080048294.01.T01 MTR_8g012090 39.483 271 102 6 1 260 53 272 3.80e-51 170
MsG0080048294.01.T01 MTR_7g016930 39.623 265 118 7 1 260 53 280 7.87e-51 169
MsG0080048294.01.T01 MTR_0129s0120 38.828 273 131 9 1 260 54 303 9.91e-51 171
MsG0080048294.01.T01 MTR_2g038680 38.768 276 141 7 1 259 53 317 1.88e-50 170
MsG0080048294.01.T01 MTR_6g086825 40.000 275 136 6 1 260 107 367 3.56e-50 171
MsG0080048294.01.T01 MTR_6g090180 41.727 278 128 8 1 260 84 345 2.72e-49 168
MsG0080048294.01.T01 MTR_1g007410 41.392 273 129 10 1 260 59 313 4.75e-49 167
MsG0080048294.01.T01 MTR_2g034700 41.791 268 111 12 1 260 51 281 8.02e-49 165
MsG0080048294.01.T01 MTR_6g461660 39.706 272 133 9 1 260 56 308 1.09e-48 166
MsG0080048294.01.T01 MTR_0129s0050 41.155 277 135 10 1 260 51 316 2.76e-48 165
MsG0080048294.01.T01 MTR_5g097350 38.745 271 122 9 4 260 56 296 3.14e-48 164
MsG0080048294.01.T01 MTR_7g017040 40.959 271 125 8 4 260 56 305 3.26e-47 162
MsG0080048294.01.T01 MTR_5g097680 39.394 297 130 10 1 260 54 337 9.60e-47 161
MsG0080048294.01.T01 MTR_5g064700 38.621 290 130 12 5 260 3 278 1.09e-46 159
MsG0080048294.01.T01 MTR_8g467670 40.226 266 123 12 1 260 51 286 9.63e-46 158
MsG0080048294.01.T01 MTR_6g079770 40.714 280 137 10 1 260 54 324 5.86e-45 156
MsG0080048294.01.T01 MTR_0134s0060 41.281 281 134 11 1 260 49 319 6.99e-45 156
MsG0080048294.01.T01 MTR_5g024260 37.956 274 127 12 1 260 59 303 3.50e-44 154
MsG0080048294.01.T01 MTR_5g037680 36.996 273 129 9 1 259 45 288 5.49e-44 154
MsG0080048294.01.T01 MTR_5g059430 37.011 281 143 8 1 259 63 331 1.56e-43 152
MsG0080048294.01.T01 MTR_4g007940 39.163 263 127 13 14 260 4 249 1.40e-42 148
MsG0080048294.01.T01 MTR_8g064890 39.098 266 133 9 1 260 53 295 1.63e-42 149
MsG0080048294.01.T01 MTR_1g053510 38.214 280 133 11 1 259 52 312 2.39e-42 149
MsG0080048294.01.T01 MTR_5g096900 38.929 280 118 13 1 259 49 296 3.72e-42 146
MsG0080048294.01.T01 MTR_8g039500 35.472 265 132 9 1 259 49 280 4.42e-42 147
MsG0080048294.01.T01 MTR_5g097220 37.184 277 138 11 4 260 53 313 4.43e-42 148
MsG0080048294.01.T01 MTR_5g069390 38.129 278 136 9 1 259 63 323 9.65e-42 149
MsG0080048294.01.T01 MTR_2g066170 35.472 265 141 9 1 259 49 289 1.25e-41 147
MsG0080048294.01.T01 MTR_0054s0090 37.363 273 145 9 1 260 54 313 2.15e-41 147
MsG0080048294.01.T01 MTR_7g111700 37.313 268 115 6 7 259 43 272 3.24e-41 145
MsG0080048294.01.T01 MTR_1g007450 39.850 266 143 8 1 260 49 303 3.44e-41 146
MsG0080048294.01.T01 MTR_4g028680 37.770 278 132 10 1 260 53 307 4.30e-41 145
MsG0080048294.01.T01 MTR_4g028510 36.786 280 133 11 1 260 54 309 4.32e-41 147
MsG0080048294.01.T01 MTR_0054s0100 37.868 272 141 8 1 257 54 312 4.41e-41 146
MsG0080048294.01.T01 MTR_6g090250 36.242 298 138 10 1 260 48 331 4.57e-41 146
MsG0080048294.01.T01 MTR_8g033140 35.739 291 132 12 1 259 56 323 5.51e-41 145
MsG0080048294.01.T01 MTR_0831s0010 38.636 264 134 10 1 259 87 327 7.40e-41 145
MsG0080048294.01.T01 MTR_8g046310 35.581 267 128 7 1 259 53 283 7.55e-41 144
MsG0080048294.01.T01 MTR_6g080680 39.068 279 119 10 1 260 56 302 8.49e-41 145
MsG0080048294.01.T01 MTR_5g076490 39.416 274 121 10 1 260 1 243 9.48e-41 143
MsG0080048294.01.T01 MTR_2g066200 33.708 267 145 8 1 259 49 291 5.08e-40 142
MsG0080048294.01.T01 MTR_4g028750 35.870 276 138 11 1 260 53 305 6.12e-40 142
MsG0080048294.01.T01 MTR_6g079810 36.713 286 133 12 1 260 107 370 1.23e-38 140
MsG0080048294.01.T01 MTR_4g130330 36.918 279 134 11 2 260 41 297 2.76e-38 138
MsG0080048294.01.T01 MTR_6g078010 35.252 278 126 9 1 260 1 242 5.08e-38 136
MsG0080048294.01.T01 MTR_1g007430 35.849 265 141 8 1 260 33 273 1.43e-37 135
MsG0080048294.01.T01 MTR_5g075370 35.842 279 131 12 6 260 51 305 2.66e-37 139
MsG0080048294.01.T01 MTR_5g097820 35.683 227 119 7 1 217 60 269 6.34e-37 131
MsG0080048294.01.T01 MTR_5g037190 37.118 229 103 6 1 219 53 250 8.30e-37 130
MsG0080048294.01.T01 MTR_8g032980 37.037 297 127 15 1 259 53 327 1.00e-36 134
MsG0080048294.01.T01 MTR_5g091140 37.079 267 126 9 4 260 88 322 1.07e-36 134
MsG0080048294.01.T01 MTR_4g014370 34.471 293 133 14 1 259 48 315 1.78e-36 134
MsG0080048294.01.T01 MTR_1g098180 36.328 256 111 10 4 259 59 262 4.27e-36 132
MsG0080048294.01.T01 MTR_4g028660 35.842 279 135 12 1 260 53 306 5.83e-36 132
MsG0080048294.01.T01 MTR_4g027450 35.842 279 135 12 1 260 53 306 5.83e-36 132
MsG0080048294.01.T01 MTR_8g063330 35.662 272 139 12 1 259 51 299 8.19e-36 133
MsG0080048294.01.T01 MTR_5g096910 37.657 239 105 10 36 259 88 297 1.46e-35 131
MsG0080048294.01.T01 MTR_5g092860 34.783 276 131 11 1 260 39 281 2.78e-35 129
MsG0080048294.01.T01 MTR_5g074850 35.461 282 134 13 1 260 74 329 3.49e-35 133
MsG0080048294.01.T01 MTR_2g065860 32.847 274 141 11 1 260 49 293 8.42e-35 129
MsG0080048294.01.T01 MTR_5g094350 36.653 251 112 9 1 231 50 273 2.82e-34 125
MsG0080048294.01.T01 MTR_8g033090 36.458 288 137 14 1 260 51 320 3.30e-34 127
MsG0080048294.01.T01 MTR_7g104710 37.319 276 106 12 1 260 54 278 3.99e-34 125
MsG0080048294.01.T01 MTR_2g065840 29.779 272 151 8 1 260 49 292 5.66e-34 126
MsG0080048294.01.T01 MTR_6g004450 42.771 166 71 5 98 259 31 176 2.34e-33 122
MsG0080048294.01.T01 MTR_5g043605 35.065 231 111 8 38 259 45 245 2.62e-33 124
MsG0080048294.01.T01 MTR_8g046200 34.201 269 120 10 7 232 54 308 4.05e-33 125
MsG0080048294.01.T01 MTR_6g478230 33.929 280 136 9 1 260 64 314 5.39e-33 124
MsG0080048294.01.T01 MTR_8g033110 34.035 285 150 10 1 260 51 322 3.60e-32 122
MsG0080048294.01.T01 MTR_8g033000 35.664 286 140 14 1 259 1 269 7.99e-32 120
MsG0080048294.01.T01 MTR_6g070880 39.196 199 98 7 2 185 70 260 9.50e-32 118
MsG0080048294.01.T01 MTR_6g071420 39.196 199 98 7 2 185 70 260 9.50e-32 118
MsG0080048294.01.T01 MTR_1g047080 34.848 264 126 11 1 258 1 224 1.62e-31 118
MsG0080048294.01.T01 MTR_5g086590 41.667 180 75 6 81 257 11 163 1.70e-31 116
MsG0080048294.01.T01 MTR_1g050312 34.071 226 103 7 37 260 16 197 4.54e-31 117
MsG0080048294.01.T01 MTR_5g092850 34.286 280 138 13 1 260 53 306 3.12e-30 117
MsG0080048294.01.T01 MTR_6g079790 34.934 229 118 6 49 260 2 216 6.53e-30 114
MsG0080048294.01.T01 MTR_5g068490 33.953 215 104 7 46 259 8 185 6.60e-30 113
MsG0080048294.01.T01 MTR_1g076810 39.409 203 96 7 1 194 55 239 2.30e-29 116
MsG0080048294.01.T01 MTR_8g046220 34.766 256 119 11 1 229 54 288 2.76e-29 114
MsG0080048294.01.T01 MTR_2g066150 31.835 267 123 9 1 260 49 263 2.04e-28 111
MsG0080048294.01.T01 MTR_2g021920 43.421 152 77 4 114 260 35 182 3.58e-28 108
MsG0080048294.01.T01 MTR_7g023800 33.212 274 109 9 4 260 2 218 4.42e-28 109
MsG0080048294.01.T01 MTR_6g087620 35.021 237 99 8 37 260 1 195 1.18e-27 107
MsG0080048294.01.T01 MTR_8g018110 33.071 254 140 11 1 235 51 293 2.08e-27 109
MsG0080048294.01.T01 MTR_5g098950 44.094 127 59 4 85 210 90 205 2.94e-27 106
MsG0080048294.01.T01 MTR_8g063370 29.323 266 152 11 1 260 61 296 4.45e-26 105
MsG0080048294.01.T01 MTR_6g465360 41.579 190 75 11 1 166 99 276 6.62e-26 103
MsG0080048294.01.T01 MTR_6g009350 40.000 150 66 6 116 259 8 139 2.51e-25 99.4
MsG0080048294.01.T01 MTR_1g115205 37.778 180 78 6 32 202 82 236 8.94e-25 99.4
MsG0080048294.01.T01 MTR_4g016000 36.000 175 81 5 88 260 2 147 9.31e-24 95.9
MsG0080048294.01.T01 MTR_8g073570 44.531 128 64 5 1 126 47 169 1.35e-23 94.4
MsG0080048294.01.T01 MTR_8g064790 42.466 146 72 5 1 140 52 191 3.86e-23 96.7
MsG0080048294.01.T01 MTR_5g091150 33.617 235 80 12 49 259 2 184 7.67e-23 93.2
MsG0080048294.01.T01 MTR_5g023970 47.525 101 48 2 159 259 6 101 1.47e-22 92.0
MsG0080048294.01.T01 MTR_8g036970 29.811 265 112 9 1 260 35 230 1.80e-21 92.4
MsG0080048294.01.T01 MTR_1g050308 34.197 193 99 8 1 187 71 241 2.10e-21 92.0
MsG0080048294.01.T01 MTR_8g063310 28.947 266 152 13 7 259 60 301 2.99e-21 92.4
MsG0080048294.01.T01 MTR_5g049560 37.952 166 87 5 1 150 1 166 5.38e-21 89.7
MsG0080048294.01.T01 MTR_8g063350 29.362 235 126 10 37 260 45 250 6.37e-20 87.8
MsG0080048294.01.T01 MTR_3g018750 37.975 158 72 6 114 259 1 144 6.80e-20 86.3
MsG0080048294.01.T01 MTR_7g101200 34.973 183 79 10 82 259 37 184 9.76e-18 80.1
MsG0080048294.01.T01 MTR_6g090260 42.718 103 49 3 162 260 73 169 3.57e-16 76.3
MsG0080048294.01.T01 MTR_3g047540 45.000 80 40 3 163 241 5 81 7.72e-16 71.2
MsG0080048294.01.T01 MTR_5g097650 39.823 113 54 4 1 111 59 159 1.30e-15 72.8
MsG0080048294.01.T01 MTR_3g030620 50.704 71 34 1 190 259 18 88 3.20e-14 69.3
MsG0080048294.01.T01 MTR_5g097850 42.391 92 46 3 172 260 66 153 1.32e-12 66.2
MsG0080048294.01.T01 MTR_4g014370 34.188 117 59 4 147 259 141 243 1.52e-12 67.0
MsG0080048294.01.T01 MTR_8g035690 27.778 198 99 8 89 260 30 209 5.73e-12 65.1
MsG0080048294.01.T01 MTR_3g058480 26.829 164 84 7 74 229 147 282 3.80e-11 62.8
MsG0080048294.01.T01 MTR_0115s0020 29.534 193 108 9 47 229 94 268 7.82e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 57 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTCTTTCTATTCACTTTC+TGG 0.014456 contig266end:+22965 MsG0080048294.01.T01:CDS
AAATATCCTATGAACCTTTA+TGG 0.145075 contig266end:+23332 MsG0080048294.01.T01:CDS
CCCAACTACCAATGAATTTA+AGG 0.172366 contig266end:+23126 MsG0080048294.01.T01:CDS
AGACATTTGGTGGTGTTTAA+TGG 0.225380 contig266end:+23601 MsG0080048294.01.T01:CDS
AAGATGACATTAAGATTATT+AGG 0.241147 contig266end:+23233 MsG0080048294.01.T01:CDS
TGTTAATCTAAAACTCAAAT+TGG 0.264418 contig266end:-23575 None:intergenic
TCGACTTTATTTCATGGATT+TGG 0.281069 contig266end:+23196 MsG0080048294.01.T01:CDS
ATTGATTGTGATATTATTAC+CGG 0.315569 contig266end:+23061 MsG0080048294.01.T01:CDS
TGAACCAGATTCAACATATT+CGG 0.317640 contig266end:+24774 MsG0080048294.01.T01:intron
GAACTATCAGTCCAACATTT+AGG 0.348474 contig266end:-23406 None:intergenic
TCTTAATGTCATCTTGAATA+TGG 0.362368 contig266end:-23225 None:intergenic
ACCTTAAATTCATTGGTAGT+TGG 0.369095 contig266end:-23127 None:intergenic
TACATCTCTCATCTTATATC+AGG 0.370233 contig266end:+22916 MsG0080048294.01.T01:CDS
AATATCCTATGAACCTTTAT+GGG 0.375214 contig266end:+23333 MsG0080048294.01.T01:CDS
GAGGCTTCGACTTTATTTCA+TGG 0.382507 contig266end:+23190 MsG0080048294.01.T01:CDS
CCTTAAATTCATTGGTAGTT+GGG 0.383028 contig266end:-23126 None:intergenic
AATGAGCGATTGTACTTGAA+TGG 0.385402 contig266end:+23430 MsG0080048294.01.T01:CDS
AAGAGGAGCTTACCCTACTA+TGG 0.385977 contig266end:-24835 None:intergenic
TATGTCTGAAGGTATGTCTA+AGG 0.395912 contig266end:-23548 None:intergenic
TAGATTAACAAAGAGACATT+TGG 0.397296 contig266end:+23588 MsG0080048294.01.T01:CDS
AATCTCCCATAAAGGTTCAT+AGG 0.399252 contig266end:-23338 None:intergenic
ACGCTATAAATCTCCCATAA+AGG 0.408323 contig266end:-23346 None:intergenic
AGGAAGGACCTTAAATTCAT+TGG 0.414976 contig266end:-23134 None:intergenic
AGTCACAATGCAGAACAAAT+AGG 0.432697 contig266end:-25034 None:intergenic
TTCATATTGTTGTAGATGCT+GGG 0.442524 contig266end:+24975 MsG0080048294.01.T01:intron
TGCTGTGATATTGCTATCGT+TGG 0.454725 contig266end:+25083 MsG0080048294.01.T01:three_prime_UTR
ATTTCAAGATGCCTAAATGT+TGG 0.461244 contig266end:+23395 MsG0080048294.01.T01:CDS
GATTCAACATATTCGGATTG+AGG 0.467640 contig266end:+24781 MsG0080048294.01.T01:intron
ATAGCGTAAGATGTAACTCT+TGG 0.474490 contig266end:+23362 MsG0080048294.01.T01:CDS
TCATAGGATATTTCATTCCA+TGG 0.482117 contig266end:-23322 None:intergenic
TCTATTCACTTTCTGGTGAG+AGG 0.486761 contig266end:+22972 MsG0080048294.01.T01:CDS
CAATCAATAAAATCAAAGAA+AGG 0.493804 contig266end:-23046 None:intergenic
GCGTAAGATGTAACTCTTGG+AGG 0.496197 contig266end:+23365 MsG0080048294.01.T01:CDS
ACTTGAATGGAATATGTCAT+TGG 0.510554 contig266end:+23443 MsG0080048294.01.T01:CDS
TTTGTTTGATGTCCATAGTA+GGG 0.511020 contig266end:+24823 MsG0080048294.01.T01:three_prime_UTR
AATCAATAAAATCAAAGAAA+GGG 0.513018 contig266end:-23045 None:intergenic
TCGACTTCTCAACGTGCGAC+GGG 0.522901 contig266end:+23291 MsG0080048294.01.T01:CDS
AAAGTCGAAGCCTCTCGATA+AGG 0.531483 contig266end:-23181 None:intergenic
TTCGACTTCTCAACGTGCGA+CGG 0.534033 contig266end:+23290 MsG0080048294.01.T01:CDS
GGTATCGACTCAGCTGAACT+AGG 0.540661 contig266end:-23157 None:intergenic
GATCCATTGCTTCGATCTCA+TGG 0.550302 contig266end:+23623 MsG0080048294.01.T01:CDS
ATTAACAAAGAGACATTTGG+TGG 0.556549 contig266end:+23591 MsG0080048294.01.T01:CDS
GTGGCATGTGACTGAAGATC+GGG 0.557383 contig266end:+23465 MsG0080048294.01.T01:CDS
CTTACCAAATCCATTTCAAG+AGG 0.561311 contig266end:+23018 MsG0080048294.01.T01:CDS
CTTCAAAAGCACGAATGCAA+TGG 0.575110 contig266end:-25007 None:intergenic
ATACCATGAGATCGAAGCAA+TGG 0.576085 contig266end:-23626 None:intergenic
ATGTCTGAAGGTATGTCTAA+GGG 0.595397 contig266end:-23547 None:intergenic
AAATTGGTATCTATGTCTGA+AGG 0.609303 contig266end:-23559 None:intergenic
TGCGACGGGAAGATGTGCCA+TGG 0.609776 contig266end:+23305 MsG0080048294.01.T01:CDS
GGTGGCATGTGACTGAAGAT+CGG 0.610968 contig266end:+23464 MsG0080048294.01.T01:CDS
TCATATTGTTGTAGATGCTG+GGG 0.613531 contig266end:+24976 MsG0080048294.01.T01:intron
GTCGAAGCCTCTCGATAAGG+TGG 0.632668 contig266end:-23178 None:intergenic
ACTCAGCTGAACTAGGAGGA+AGG 0.637957 contig266end:-23150 None:intergenic
TGAATGGAATATGTCATTGG+TGG 0.650004 contig266end:+23446 MsG0080048294.01.T01:CDS
GTCGATACCACCTTATCGAG+AGG 0.653573 contig266end:+23171 MsG0080048294.01.T01:CDS
ATTTAGATATGAAGTTAGTG+CGG 0.670374 contig266end:-22874 None:intergenic
ATCGACTCAGCTGAACTAGG+AGG 0.685967 contig266end:-23154 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAAAAATGATTTATATA+TGG + contig266end:24401-24420 MsG0080048294.01.T01:intron 10.0%
!! ATATATAATTCTAATAACAA+TGG - contig266end:24130-24149 None:intergenic 10.0%
!!! ATAAATCATTTTTTCTTTAA+AGG - contig266end:24398-24417 None:intergenic 10.0%
!! AATCAATAAAATCAAAGAAA+GGG - contig266end:23048-23067 None:intergenic 15.0%
!! TATTTCCTAAAACAAAAAAA+TGG + contig266end:23775-23794 MsG0080048294.01.T01:intron 15.0%
!! TTTGTCATTAATAATTTAGA+GGG - contig266end:24612-24631 None:intergenic 15.0%
!!! CAACTATTTCTATATAAAAT+TGG - contig266end:24173-24192 None:intergenic 15.0%
!!! TTTGATAATATTTATGATCA+GGG - contig266end:23989-24008 None:intergenic 15.0%
!!! TTTTGTCATTAATAATTTAG+AGG - contig266end:24613-24632 None:intergenic 15.0%
!! AACACAATCATAAATTTCTA+AGG - contig266end:23940-23959 None:intergenic 20.0%
!! AAGATGACATTAAGATTATT+AGG + contig266end:23233-23252 MsG0080048294.01.T01:CDS 20.0%
!! AATTTATGATTGTGTTTAGT+AGG + contig266end:23944-23963 MsG0080048294.01.T01:intron 20.0%
!! ATTGATTGTGATATTATTAC+CGG + contig266end:23061-23080 MsG0080048294.01.T01:CDS 20.0%
!! CAATCAATAAAATCAAAGAA+AGG - contig266end:23049-23068 None:intergenic 20.0%
!! TATTTGCACATTTAATGAAA+TGG - contig266end:24492-24511 None:intergenic 20.0%
!! TGAATTTCAATTAATTATGC+TGG + contig266end:24237-24256 MsG0080048294.01.T01:intron 20.0%
!! TTAAAGCATAAACTTAAACA+TGG - contig266end:24277-24296 None:intergenic 20.0%
!!! CTTTGATAATATTTATGATC+AGG - contig266end:23990-24009 None:intergenic 20.0%
!!! TACTTTTCACATATCAATTT+AGG + contig266end:23660-23679 MsG0080048294.01.T01:intron 20.0%
!!! TGTTAATCTAAAACTCAAAT+TGG - contig266end:23578-23597 None:intergenic 20.0%
! AAATATCCTATGAACCTTTA+TGG + contig266end:23332-23351 MsG0080048294.01.T01:CDS 25.0%
! AACAAAAAACACTTGTATTG+TGG + contig266end:23101-23120 MsG0080048294.01.T01:CDS 25.0%
! AATATCCTATGAACCTTTAT+GGG + contig266end:23333-23352 MsG0080048294.01.T01:CDS 25.0%
! ATTGAAATTCAAACAACCAT+AGG - contig266end:24228-24247 None:intergenic 25.0%
! ATTTAGATATGAAGTTAGTG+CGG - contig266end:22877-22896 None:intergenic 25.0%
! TAGATTAACAAAGAGACATT+TGG + contig266end:23588-23607 MsG0080048294.01.T01:CDS 25.0%
! TCTTAATGTCATCTTGAATA+TGG - contig266end:23228-23247 None:intergenic 25.0%
! TTGCAAGCAAAAATATTTGT+AGG + contig266end:24338-24357 MsG0080048294.01.T01:intron 25.0%
!! TTCTCTTTCTATTTTCAAAC+TGG + contig266end:24090-24109 MsG0080048294.01.T01:intron 25.0%
!!! ACTGACCATTTTTTTGTTTT+AGG - contig266end:23783-23802 None:intergenic 25.0%
!!! CAAGTGTTTTTTGTTTTTTG+AGG - contig266end:23096-23115 None:intergenic 25.0%
AAATTGGTATCTATGTCTGA+AGG - contig266end:23562-23581 None:intergenic 30.0%
AAGCATAAACTTAAACATGG+TGG - contig266end:24274-24293 None:intergenic 30.0%
ACTTGAATGGAATATGTCAT+TGG + contig266end:23443-23462 MsG0080048294.01.T01:CDS 30.0%
ATAATCTAACAAGCAACCTA+TGG + contig266end:24209-24228 MsG0080048294.01.T01:intron 30.0%
ATTAACAAAGAGACATTTGG+TGG + contig266end:23591-23610 MsG0080048294.01.T01:CDS 30.0%
ATTGGAGACAGTTCAATAAA+AGG - contig266end:24155-24174 None:intergenic 30.0%
ATTTCAAGATGCCTAAATGT+TGG + contig266end:23395-23414 MsG0080048294.01.T01:CDS 30.0%
CATATCAATTTAGGTGAACT+CGG + contig266end:23669-23688 MsG0080048294.01.T01:intron 30.0%
TACATCTCTCATCTTATATC+AGG + contig266end:22916-22935 MsG0080048294.01.T01:CDS 30.0%
TCATAGGATATTTCATTCCA+TGG - contig266end:23325-23344 None:intergenic 30.0%
TGAACCAGATTCAACATATT+CGG + contig266end:24774-24793 MsG0080048294.01.T01:intron 30.0%
TTCATATTGTTGTAGATGCT+GGG + contig266end:24975-24994 MsG0080048294.01.T01:intron 30.0%
TTTGTTTGATGTCCATAGTA+GGG + contig266end:24823-24842 MsG0080048294.01.T01:three_prime_UTR 30.0%
! ACCTTAAATTCATTGGTAGT+TGG - contig266end:23130-23149 None:intergenic 30.0%
! CCTTAAATTCATTGGTAGTT+GGG - contig266end:23129-23148 None:intergenic 30.0%
! GTGCATTTTTGTCGAATAAA+TGG + contig266end:23259-23278 MsG0080048294.01.T01:CDS 30.0%
! TCGACTTTATTTCATGGATT+TGG + contig266end:23196-23215 MsG0080048294.01.T01:CDS 30.0%
! TTTCTATTTTCAAACTGGTG+TGG + contig266end:24095-24114 MsG0080048294.01.T01:intron 30.0%
!! TTTTCCTCTTGAAATGGATT+TGG - contig266end:23025-23044 None:intergenic 30.0%
!! TTTTGTTTGATGTCCATAGT+AGG + contig266end:24822-24841 MsG0080048294.01.T01:three_prime_UTR 30.0%
!!! TTTTTTGAGGCAAAGAATTC+CGG - contig266end:23083-23102 None:intergenic 30.0%
AATCTCCCATAAAGGTTCAT+AGG - contig266end:23341-23360 None:intergenic 35.0%
AATGAGCGATTGTACTTGAA+TGG + contig266end:23430-23449 MsG0080048294.01.T01:CDS 35.0%
AATTGCAGATCATTCTGATC+AGG + contig266end:24558-24577 MsG0080048294.01.T01:intron 35.0%
ACGCTATAAATCTCCCATAA+AGG - contig266end:23349-23368 None:intergenic 35.0%
ACTGAAAAACTTGGGTGTAT+GGG + contig266end:23831-23850 MsG0080048294.01.T01:intron 35.0%
AGGAAGGACCTTAAATTCAT+TGG - contig266end:23137-23156 None:intergenic 35.0%
AGTCACAATGCAGAACAAAT+AGG - contig266end:25037-25056 None:intergenic 35.0%
ATAGCGTAAGATGTAACTCT+TGG + contig266end:23362-23381 MsG0080048294.01.T01:CDS 35.0%
CAATCCGAATATGTTGAATC+TGG - contig266end:24781-24800 None:intergenic 35.0%
CCCAACTACCAATGAATTTA+AGG + contig266end:23126-23145 MsG0080048294.01.T01:CDS 35.0%
CTACAACAATATGAACGAGT+CGG - contig266end:24970-24989 None:intergenic 35.0%
CTTACCAAATCCATTTCAAG+AGG + contig266end:23018-23037 MsG0080048294.01.T01:CDS 35.0%
CTTATGATCATAGACGATAC+AGG + contig266end:24712-24731 MsG0080048294.01.T01:intron 35.0%
GAACTATCAGTCCAACATTT+AGG - contig266end:23409-23428 None:intergenic 35.0%
GATTCAACATATTCGGATTG+AGG + contig266end:24781-24800 MsG0080048294.01.T01:intron 35.0%
GTTCATATTGTTGTAGATGC+TGG + contig266end:24974-24993 MsG0080048294.01.T01:intron 35.0%
TACAACAATATGAACGAGTC+GGG - contig266end:24969-24988 None:intergenic 35.0%
TACTGATGATCATGCTTTCT+CGG + contig266end:24368-24387 MsG0080048294.01.T01:intron 35.0%
TAGAGGGCTTAAAAAAGAAC+AGG - contig266end:24596-24615 None:intergenic 35.0%
TCATATTGTTGTAGATGCTG+GGG + contig266end:24976-24995 MsG0080048294.01.T01:intron 35.0%
TGAATGGAATATGTCATTGG+TGG + contig266end:23446-23465 MsG0080048294.01.T01:CDS 35.0%
TGCTCTTTCTATTCACTTTC+TGG + contig266end:22965-22984 MsG0080048294.01.T01:CDS 35.0%
TTATTCCTCCAATCGAAACA+AGG - contig266end:23890-23909 None:intergenic 35.0%
! AAAGGGTTTTCCTCTTGAAA+TGG - contig266end:23031-23050 None:intergenic 35.0%
! ATGTCTGAAGGTATGTCTAA+GGG - contig266end:23550-23569 None:intergenic 35.0%
! ATGTTGAGACTCTGAAAACA+TGG + contig266end:24914-24933 MsG0080048294.01.T01:intron 35.0%
! TATGTCTGAAGGTATGTCTA+AGG - contig266end:23551-23570 None:intergenic 35.0%
! TTTTGTCGAATAAATGGTCG+TGG + contig266end:23265-23284 MsG0080048294.01.T01:CDS 35.0%
!! AAGAAAAGCCTTGTTTCGAT+TGG + contig266end:23879-23898 MsG0080048294.01.T01:intron 35.0%
!! AGACATTTGGTGGTGTTTAA+TGG + contig266end:23601-23620 MsG0080048294.01.T01:CDS 35.0%
!!! ACTGTTGCTTTTAGGTCTTT+TGG + contig266end:25054-25073 MsG0080048294.01.T01:three_prime_UTR 35.0%
!!! AGATCGGGATGAACATTTTT+TGG + contig266end:23480-23499 MsG0080048294.01.T01:CDS 35.0%
ACCCAGAAGACTGAAAAACT+TGG + contig266end:23822-23841 MsG0080048294.01.T01:intron 40.0%
ATACCATGAGATCGAAGCAA+TGG - contig266end:23629-23648 None:intergenic 40.0%
CCCAGAAGACTGAAAAACTT+GGG + contig266end:23823-23842 MsG0080048294.01.T01:intron 40.0%
CTCTACATTGAGCGTCTTAT+TGG + contig266end:23735-23754 MsG0080048294.01.T01:intron 40.0%
CTGTTACTGTCCTTGGTTTA+GGG + contig266end:24662-24681 MsG0080048294.01.T01:intron 40.0%
GACTGAAAAACTTGGGTGTA+TGG + contig266end:23830-23849 MsG0080048294.01.T01:intron 40.0%
TCTTATTGGAGTGGGAAGAA+CGG + contig266end:23749-23768 MsG0080048294.01.T01:intron 40.0%
TGATCAACCACAGTGTAAAG+AGG - contig266end:24855-24874 None:intergenic 40.0%
TGCTGTGATATTGCTATCGT+TGG + contig266end:25083-25102 MsG0080048294.01.T01:three_prime_UTR 40.0%
TGGTCCAACAACAAAGAGTT+TGG - contig266end:23712-23731 None:intergenic 40.0%
! ACAGCACTTTGGAAGGAAAA+TGG - contig266end:24646-24665 None:intergenic 40.0%
! ATTGAGCGTCTTATTGGAGT+GGG + contig266end:23741-23760 MsG0080048294.01.T01:intron 40.0%
! CTTCAAAAGCACGAATGCAA+TGG - contig266end:25010-25029 None:intergenic 40.0%
! GAGGCTTCGACTTTATTTCA+TGG + contig266end:23190-23209 MsG0080048294.01.T01:CDS 40.0%
! TCTATTCACTTTCTGGTGAG+AGG + contig266end:22972-22991 MsG0080048294.01.T01:CDS 40.0%
! TGGACCAAACTCTTTGTTGT+TGG + contig266end:23705-23724 MsG0080048294.01.T01:intron 40.0%
!! AAAAGCCTTGTTTCGATTGG+AGG + contig266end:23882-23901 MsG0080048294.01.T01:intron 40.0%
!! ACCCAAGTTTTTCAGTCTTC+TGG - contig266end:23827-23846 None:intergenic 40.0%
!! CCCAAGTTTTTCAGTCTTCT+GGG - contig266end:23826-23845 None:intergenic 40.0%
!! TTGCATTCGTGCTTTTGAAG+TGG + contig266end:25010-25029 MsG0080048294.01.T01:three_prime_UTR 40.0%
!!! GCATTGTGACTGTTGCTTTT+AGG + contig266end:25046-25065 MsG0080048294.01.T01:three_prime_UTR 40.0%
!!! TGCATTCGTGCTTTTGAAGT+GGG + contig266end:25011-25030 MsG0080048294.01.T01:three_prime_UTR 40.0%
AAAGTCGAAGCCTCTCGATA+AGG - contig266end:23184-23203 None:intergenic 45.0%
AAGAGGAGCTTACCCTACTA+TGG - contig266end:24838-24857 None:intergenic 45.0%
AATATGAACGAGTCGGGAAG+AGG - contig266end:24963-24982 None:intergenic 45.0%
AGACGCTCAATGTAGAGCAA+TGG - contig266end:23732-23751 None:intergenic 45.0%
ATGCAATTGTCGTACATGCC+AGG + contig266end:24883-24902 MsG0080048294.01.T01:intron 45.0%
CAAGGACAGTAACAGCACTT+TGG - contig266end:24657-24676 None:intergenic 45.0%
CTGTCCTTGGTTTAGGGAAA+AGG + contig266end:24668-24687 MsG0080048294.01.T01:intron 45.0%
GACAGTAACAGCACTTTGGA+AGG - contig266end:24653-24672 None:intergenic 45.0%
GATCCATTGCTTCGATCTCA+TGG + contig266end:23623-23642 MsG0080048294.01.T01:CDS 45.0%
GCGTAAGATGTAACTCTTGG+AGG + contig266end:23365-23384 MsG0080048294.01.T01:CDS 45.0%
GCTGTTACTGTCCTTGGTTT+AGG + contig266end:24661-24680 MsG0080048294.01.T01:intron 45.0%
GTAAGCTCCTCTTTACACTG+TGG + contig266end:24845-24864 MsG0080048294.01.T01:intron 45.0%
TGCAATTGTCGTACATGCCA+GGG + contig266end:24884-24903 MsG0080048294.01.T01:intron 45.0%
! CATTGAGCGTCTTATTGGAG+TGG + contig266end:23740-23759 MsG0080048294.01.T01:intron 45.0%
! CTCTCCTTTTCCCTAAACCA+AGG - contig266end:24675-24694 None:intergenic 45.0%
!! CAAAGTGCTGTTACTGTCCT+TGG + contig266end:24655-24674 MsG0080048294.01.T01:intron 45.0%
ACTCAGCTGAACTAGGAGGA+AGG - contig266end:23153-23172 None:intergenic 50.0%
GGTGGCATGTGACTGAAGAT+CGG + contig266end:23464-23483 MsG0080048294.01.T01:CDS 50.0%
GTCGATACCACCTTATCGAG+AGG + contig266end:23171-23190 MsG0080048294.01.T01:CDS 50.0%
GTGGCATGTGACTGAAGATC+GGG + contig266end:23465-23484 MsG0080048294.01.T01:CDS 50.0%
TTCGACTTCTCAACGTGCGA+CGG + contig266end:23290-23309 MsG0080048294.01.T01:CDS 50.0%
TTTAGGTGAACTCGGCGTGA+AGG + contig266end:23677-23696 MsG0080048294.01.T01:intron 50.0%
! ATCGACTCAGCTGAACTAGG+AGG - contig266end:23157-23176 None:intergenic 50.0%
!! GGTATCGACTCAGCTGAACT+AGG - contig266end:23160-23179 None:intergenic 50.0%
AACTCGGCGTGAAGGAATCG+TGG + contig266end:23685-23704 MsG0080048294.01.T01:intron 55.0%
GTCGAAGCCTCTCGATAAGG+TGG - contig266end:23181-23200 None:intergenic 55.0%
GTCTCAACATTGCGTCTCCC+TGG - contig266end:24904-24923 None:intergenic 55.0%
TCGACTTCTCAACGTGCGAC+GGG + contig266end:23291-23310 MsG0080048294.01.T01:CDS 55.0%
TGCGACGGGAAGATGTGCCA+TGG + contig266end:23305-23324 MsG0080048294.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
contig266end gene 22863 25131 22863 ID=MsG0080048294.01;Name=MsG0080048294.01
contig266end mRNA 22863 25131 22863 ID=MsG0080048294.01.T01;Parent=MsG0080048294.01;Name=MsG0080048294.01.T01;_AED=0.57;_eAED=0.57;_QI=0|0|0|0.33|1|1|3|204|260
contig266end exon 22863 23644 22863 ID=MsG0080048294.01.T01:exon:11591;Parent=MsG0080048294.01.T01
contig266end exon 24782 24844 24782 ID=MsG0080048294.01.T01:exon:11592;Parent=MsG0080048294.01.T01
contig266end exon 24990 25131 24990 ID=MsG0080048294.01.T01:exon:11593;Parent=MsG0080048294.01.T01
contig266end CDS 22863 23644 22863 ID=MsG0080048294.01.T01:cds;Parent=MsG0080048294.01.T01
contig266end CDS 24782 24782 24782 ID=MsG0080048294.01.T01:cds;Parent=MsG0080048294.01.T01
contig266end three_prime_UTR 24783 24844 24783 ID=MsG0080048294.01.T01:three_prime_utr;Parent=MsG0080048294.01.T01
contig266end three_prime_UTR 24990 25131 24990 ID=MsG0080048294.01.T01:three_prime_utr;Parent=MsG0080048294.01.T01
Gene Sequence

>MsG0080048294.01.T01

ATGAACAAGTTCCGCACTAACTTCATATCTAAATCTCACTCTTACTACAATGATACATCTCTCATCTTATATCAGGTTATCGATTCTCATGACTCTCTTCATTGCTCTTTCTATTCACTTTCTGGTGAGAGGTTTGAGAATAGAGTCAAATTAGACTTACCAAATCCATTTCAAGAGGAAAACCCTTTCTTTGATTTTATTGATTGTGATATTATTACCGGAATTCTTTGCCTCAAAAAACAAAAAACACTTGTATTGTGGAACCCAACTACCAATGAATTTAAGGTCCTTCCTCCTAGTTCAGCTGAGTCGATACCACCTTATCGAGAGGCTTCGACTTTATTTCATGGATTTGGTTATGACCATATTCAAGATGACATTAAGATTATTAGGTATGTGCATTTTTGTCGAATAAATGGTCGTGGTCTTCGACTTCTCAACGTGCGACGGGAAGATGTGCCATGGAATGAAATATCCTATGAACCTTTATGGGAGATTTATAGCGTAAGATGTAACTCTTGGAGGAAACTTGATTTCAAGATGCCTAAATGTTGGACTGATAGTTCAAATGAGCGATTGTACTTGAATGGAATATGTCATTGGTGGCATGTGACTGAAGATCGGGATGAACATTTTTTGGTGTCATTTGACTTTAGCAATGAGATGTTTTTTACAACAAAGATACCCTTAGACATACCTTCAGACATAGATACCAATTTGAGTTTTAGATTAACAAAGAGACATTTGGTGGTGTTTAATGGATCCATTGCTTCGATCTCATGA
Protein sequence

>MsG0080048294.01.T01

MNKFRTNFISKSHSYYNDTSLILYQVIDSHDSLHCSFYSLSGERFENRVKLDLPNPFQEENPFFDFIDCDIITGILCLKKQKTLVLWNPTTNEFKVLPPSSAESIPPYREASTLFHGFGYDHIQDDIKIIRYVHFCRINGRGLRLLNVRREDVPWNEISYEPLWEIYSVRCNSWRKLDFKMPKCWTDSSNERLYLNGICHWWHVTEDRDEHFLVSFDFSNEMFFTTKIPLDIPSDIDTNLSFRLTKRHLVVFNGSIASIS*