Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019270.01.T01 | XP_039688882.1 | 81.319 | 364 | 65 | 3 | 1 | 361 | 1 | 364 | 0 | 596 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019270.01.T01 | A0A396I3P7 | 81.319 | 364 | 65 | 3 | 1 | 361 | 1 | 364 | 0.0 | 596 |
Co-expression Network
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019270.01.T01 | MTR_4g028510 | 81.319 | 364 | 65 | 3 | 1 | 361 | 1 | 364 | 0.0 | 596 |
| MsG0480019270.01.T01 | MTR_4g028660 | 79.834 | 362 | 71 | 2 | 1 | 361 | 1 | 361 | 0.0 | 594 |
| MsG0480019270.01.T01 | MTR_4g027450 | 79.834 | 362 | 71 | 2 | 1 | 361 | 1 | 361 | 0.0 | 594 |
| MsG0480019270.01.T01 | MTR_4g028680 | 80.165 | 363 | 69 | 3 | 1 | 361 | 1 | 362 | 0.0 | 583 |
| MsG0480019270.01.T01 | MTR_4g028750 | 70.523 | 363 | 102 | 4 | 1 | 361 | 1 | 360 | 1.38e-180 | 506 |
| MsG0480019270.01.T01 | MTR_4g130330 | 68.362 | 354 | 104 | 3 | 12 | 361 | 3 | 352 | 4.29e-173 | 487 |
| MsG0480019270.01.T01 | MTR_6g079810 | 61.022 | 372 | 125 | 6 | 5 | 361 | 59 | 425 | 1.35e-145 | 420 |
| MsG0480019270.01.T01 | MTR_0054s0100 | 58.649 | 370 | 140 | 5 | 2 | 361 | 4 | 370 | 3.92e-143 | 412 |
| MsG0480019270.01.T01 | MTR_0054s0090 | 59.189 | 370 | 136 | 6 | 2 | 361 | 4 | 368 | 6.97e-143 | 411 |
| MsG0480019270.01.T01 | MTR_0134s0060 | 57.937 | 378 | 135 | 6 | 4 | 361 | 1 | 374 | 3.52e-142 | 409 |
Find 84 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACAATCATCTAAGTATGA+GGG | 0.586128 | 4:+17500614 | None:intergenic |
| AAAGCCCATTCCTCCCACCC+CGG | 0.611077 | 4:-17501011 | MsG0480019270.01.T01:CDS |
| AAATTGTAGGGGTTTGCATA+TGG | 0.388917 | 4:+17501133 | None:intergenic |
| AAGCCCATTCCTCCCACCCC+GGG | 0.554120 | 4:-17501010 | MsG0480019270.01.T01:CDS |
| AAGCTATATATCTCCCAGAA+TGG | 0.515315 | 4:+17500821 | None:intergenic |
| AAGGAGAAATATTCTTCATC+AGG | 0.428216 | 4:-17500403 | MsG0480019270.01.T01:CDS |
| AAGTAAAAGGATACCAAACA+TGG | 0.678525 | 4:+17500668 | None:intergenic |
| AATAGGCAATTTAGATAGAA+TGG | 0.360830 | 4:+17501381 | None:intergenic |
| AATATGGTATAAAGGAATCA+TGG | 0.495415 | 4:-17500487 | MsG0480019270.01.T01:CDS |
| AATCGCTTAATAGATTTAAT+AGG | 0.262034 | 4:+17501364 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGGAGAAATATTCTTCATC+AGG | - | Chr4:17501424-17501443 | MsG0480019270.01.T01:CDS | 30.0% | |
| AAGTAAAAGGATACCAAACA+TGG | + | Chr4:17501162-17501181 | None:intergenic | 30.0% | |
| AGCTATATCATCAGGTATAT+AGG | + | Chr4:17500422-17500441 | None:intergenic | 30.0% | |
| ATGATATGAGATCAATGCAA+TGG | + | Chr4:17501283-17501302 | None:intergenic | 30.0% | |
| GAAACAATCATCTAAGTATG+AGG | + | Chr4:17501217-17501236 | None:intergenic | 30.0% | |
| TAAGAAAATGCTCCACATTT+GGG | + | Chr4:17500864-17500883 | None:intergenic | 30.0% | |
| TATATCATCAGGTATATAGG+TGG | + | Chr4:17500419-17500438 | None:intergenic | 30.0% | |
| TCTCCATCATGATCATATTT+GGG | - | Chr4:17500756-17500775 | MsG0480019270.01.T01:CDS | 30.0% | |
| TTTGGGTGAATATGGTATAA+AGG | - | Chr4:17501332-17501351 | MsG0480019270.01.T01:CDS | 30.0% | |
| AAATTGTAGGGGTTTGCATA+TGG | + | Chr4:17500697-17500716 | None:intergenic | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 17500379 | 17501470 | 17500379 | ID=MsG0480019270.01;Name=MsG0480019270.01 |
| Chr4 | mRNA | 17500379 | 17501470 | 17500379 | ID=MsG0480019270.01.T01;Parent=MsG0480019270.01;Name=MsG0480019270.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|363 |
| Chr4 | exon | 17500379 | 17501470 | 17500379 | ID=MsG0480019270.01.T01:exon:4583;Parent=MsG0480019270.01.T01 |
| Chr4 | CDS | 17500379 | 17501470 | 17500379 | ID=MsG0480019270.01.T01:cds;Parent=MsG0480019270.01.T01 |
| Gene Sequence |
>MsG0480019270.01.T01 ATGGAGTCAGTGGCCGCAAAAAAAAGAAAGGTGAGCACCACCTATATACCTGATGATATAGCTTTCTCCATTCTATCTAAATTGCCTATTAAATCTATTAAGCGATTTGAATGTGTTCGCAAATCATGGTCTCGCTTATCTAAGACCCATCGTTTCATGAAAATGTTTCGCAATAATCTCTTGTCTAATTCTCACCGATGTGCTTATTACGACGGGGTATCTCTGCTCCTAAAGGATTTAGAACTTGGTAATGACGTTTTCTATTCTATTTTTGGTGAAGGGTTTGAGAACAAAGTTAAATTAGATTTCTCAAATCCATATGCAAACCCCTACAATTTTCGTATTTTTGGTTTTGGTAGTATTAATGGCACACTTTGTCTCCATCATGATCATATTTGGGGCAAAATTATATTGTGGAACCCATCGACCCATGCAATAAAGCCCATTCCTCCCACCCCGGGTGAGTTAGTTGAGTCGGGTATCCCAAATGTGGAGCATTTTCTTACTATACATGATACGTTTTATCTTCATGGATTTGGTTACGATGATCTTAGGAATGACTATAATGTCATTTGTTATGTATCTCTTAGTGGTGAACATGCTGGCTTTGGAGATATGTCTTTAGACCCATTCTGGGAGATATATAGCTTAAGAACTGACTCATGGAGGATACTTGATGTTGACATGCCTTTTTCCTTGGCATGTATTGACGGCACTCAAGTCTACATGGATGGTGTGTGTCATTGGTTGGCTGAAGAAGTTGATGATTCCCTGGAGGGACCATGTTTGGTATCCTTTTACTTGAGCAATGAGGTGTTCTTCATAACATACATACCCTCATACTTAGATGATTGTTTCGATCTTCACACATTGTGGATAAACTTGGCGGTGTTAAACGGGTCCATTGCATTGATCTCATATCATGAAAAGACGACTAAGTTTCACATATCAATTTTGGGTGAATATGGTATAAAGGAATCATGGACCAAACTCTTCATTGTTGGACCGTTGTCTTGCATTGAGCGTCCTATCGGAGTGGGTACGAAAGGAGAAATATTCTTCATCAGGAAAGATAAAGAACTATTTTCTTGA |
| Protein sequence |
>MsG0480019270.01.T01 MESVAAKKRKVSTTYIPDDIAFSILSKLPIKSIKRFECVRKSWSRLSKTHRFMKMFRNNLLSNSHRCAYYDGVSLLLKDLELGNDVFYSIFGEGFENKVKLDFSNPYANPYNFRIFGFGSINGTLCLHHDHIWGKIILWNPSTHAIKPIPPTPGELVESGIPNVEHFLTIHDTFYLHGFGYDDLRNDYNVICYVSLSGEHAGFGDMSLDPFWEIYSLRTDSWRILDVDMPFSLACIDGTQVYMDGVCHWLAEEVDDSLEGPCLVSFYLSNEVFFITYIPSYLDDCFDLHTLWINLAVLNGSIALISYHEKTTKFHISILGEYGIKESWTKLFIVGPLSCIERPIGVGTKGEIFFIRKDKELFS* |
