AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680035289.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035289.01.T01 MTR_0129s0050 71.517 323 73 4 1 314 53 365 1.35e-151 431
MsG0680035289.01.T01 MTR_0129s0070 58.360 317 112 7 1 315 56 354 1.09e-113 335
MsG0680035289.01.T01 MTR_0129s0120 56.467 317 118 7 1 315 56 354 5.93e-108 320
MsG0680035289.01.T01 MTR_4g117550 52.038 319 106 8 1 315 56 331 3.72e-93 281
MsG0680035289.01.T01 MTR_8g063440 45.938 320 139 10 1 315 51 341 1.83e-74 234
MsG0680035289.01.T01 MTR_4g055820 46.429 308 151 10 8 312 62 358 8.03e-72 226
MsG0680035289.01.T01 MTR_2g022000 42.943 333 160 10 1 315 55 375 1.24e-71 228
MsG0680035289.01.T01 MTR_2g021800 42.342 333 163 9 1 315 55 376 2.00e-71 227
MsG0680035289.01.T01 MTR_2g104340 43.963 323 155 14 2 315 50 355 1.05e-67 217
MsG0680035289.01.T01 MTR_8g012090 41.945 329 117 8 1 315 55 323 4.16e-67 213
MsG0680035289.01.T01 MTR_2g038680 43.902 328 155 11 1 314 55 367 6.35e-67 215
MsG0680035289.01.T01 MTR_5g097330 42.296 331 150 11 1 315 55 360 3.99e-66 213
MsG0680035289.01.T01 MTR_1g007450 44.479 326 148 9 1 315 51 354 9.46e-66 213
MsG0680035289.01.T01 MTR_4g014370 40.401 349 143 12 1 315 50 367 9.35e-65 209
MsG0680035289.01.T01 MTR_0054s0090 41.212 330 158 10 1 315 56 364 3.92e-64 208
MsG0680035289.01.T01 MTR_0134s0060 41.964 336 156 9 2 315 52 370 8.14e-64 207
MsG0680035289.01.T01 MTR_8g464770 43.279 305 139 8 14 315 65 338 1.08e-63 206
MsG0680035289.01.T01 MTR_7g069550 40.752 319 167 11 1 312 53 356 2.98e-63 205
MsG0680035289.01.T01 MTR_2g021770 40.741 324 168 12 1 312 51 362 4.47e-63 205
MsG0680035289.01.T01 MTR_7g017170 43.218 317 140 14 1 305 57 345 1.30e-62 204
MsG0680035289.01.T01 MTR_7g017130 43.218 317 140 14 1 305 57 345 1.70e-62 203
MsG0680035289.01.T01 MTR_6g086825 40.785 331 157 8 1 314 109 417 3.41e-62 204
MsG0680035289.01.T01 MTR_6g079770 43.976 332 157 9 1 315 56 375 3.57e-62 203
MsG0680035289.01.T01 MTR_6g079810 42.378 328 161 12 1 315 109 421 5.13e-62 204
MsG0680035289.01.T01 MTR_0054s0100 40.841 333 157 12 1 315 56 366 6.22e-62 202
MsG0680035289.01.T01 MTR_1g007430 42.006 319 152 9 1 315 35 324 1.09e-61 199
MsG0680035289.01.T01 MTR_6g090180 41.411 326 165 10 1 315 86 396 3.05e-61 201
MsG0680035289.01.T01 MTR_5g097220 40.240 333 161 11 1 315 52 364 3.55e-61 200
MsG0680035289.01.T01 MTR_8g041270 42.683 328 161 13 2 315 51 365 3.80e-61 200
MsG0680035289.01.T01 MTR_5g074800 40.379 317 168 13 1 312 1 301 4.27e-61 198
MsG0680035289.01.T01 MTR_4g028510 42.357 314 144 9 14 315 72 360 5.76e-61 201
MsG0680035289.01.T01 MTR_2g021950 40.062 322 165 8 1 314 55 356 2.17e-60 198
MsG0680035289.01.T01 MTR_2g104280 39.940 333 163 13 2 315 50 364 3.10e-60 197
MsG0680035289.01.T01 MTR_0831s0010 40.187 321 154 14 1 314 89 378 1.61e-59 196
MsG0680035289.01.T01 MTR_4g102410 43.448 290 137 10 34 314 136 407 1.86e-59 197
MsG0680035289.01.T01 MTR_8g013000 42.216 334 145 16 1 315 49 353 3.29e-59 195
MsG0680035289.01.T01 MTR_3g011120 41.745 321 144 15 2 315 54 338 6.03e-59 193
MsG0680035289.01.T01 MTR_4g028680 42.121 330 150 12 1 315 55 358 7.33e-59 194
MsG0680035289.01.T01 MTR_4g028750 41.534 313 145 9 14 315 71 356 2.06e-58 193
MsG0680035289.01.T01 MTR_5g059430 39.645 338 162 13 1 315 65 383 2.12e-58 194
MsG0680035289.01.T01 MTR_4g007940 38.710 310 164 9 11 312 6 297 4.12e-58 190
MsG0680035289.01.T01 MTR_1g007410 43.125 320 159 12 1 315 61 362 7.95e-58 191
MsG0680035289.01.T01 MTR_8g467670 40.909 308 141 10 13 315 66 337 8.45e-58 191
MsG0680035289.01.T01 MTR_5g037680 40.541 333 141 15 1 315 47 340 1.64e-57 192
MsG0680035289.01.T01 MTR_5g096630 39.458 332 167 11 1 315 57 371 6.69e-57 189
MsG0680035289.01.T01 MTR_2g066170 41.883 308 139 12 16 314 67 343 8.68e-57 188
MsG0680035289.01.T01 MTR_2g066200 41.853 313 138 13 14 314 65 345 2.21e-56 187
MsG0680035289.01.T01 MTR_4g028660 40.575 313 149 9 14 315 71 357 2.24e-56 187
MsG0680035289.01.T01 MTR_4g027450 40.575 313 149 9 14 315 71 357 2.24e-56 187
MsG0680035289.01.T01 MTR_7g079640 38.769 325 144 12 1 314 52 332 4.05e-56 186
MsG0680035289.01.T01 MTR_6g078010 39.423 312 142 7 14 315 19 293 1.17e-55 184
MsG0680035289.01.T01 MTR_5g097350 41.237 291 132 11 27 314 91 345 1.20e-55 185
MsG0680035289.01.T01 MTR_6g080680 38.739 333 149 11 1 315 58 353 1.24e-55 186
MsG0680035289.01.T01 MTR_5g069390 37.764 331 170 11 1 315 65 375 9.61e-55 186
MsG0680035289.01.T01 MTR_7g017040 42.056 321 138 13 11 315 68 356 3.41e-54 182
MsG0680035289.01.T01 MTR_6g461660 39.877 326 158 11 1 314 58 357 5.13e-54 181
MsG0680035289.01.T01 MTR_4g130330 39.873 316 151 8 14 315 58 348 6.77e-54 181
MsG0680035289.01.T01 MTR_8g063330 38.050 318 169 10 1 312 53 348 7.36e-54 183
MsG0680035289.01.T01 MTR_2g034700 37.963 324 148 9 1 315 53 332 1.59e-53 179
MsG0680035289.01.T01 MTR_6g090250 40.367 327 154 11 14 312 66 379 2.09e-53 180
MsG0680035289.01.T01 MTR_8g039500 40.411 292 139 10 29 314 72 334 3.37e-53 178
MsG0680035289.01.T01 MTR_5g096910 40.268 298 130 12 32 315 87 350 3.84e-53 179
MsG0680035289.01.T01 MTR_6g478230 37.805 328 163 6 1 315 66 365 4.51e-53 179
MsG0680035289.01.T01 MTR_2g064950 39.385 325 162 13 1 315 49 348 7.71e-52 176
MsG0680035289.01.T01 MTR_2g065860 38.245 319 141 12 14 314 65 345 2.41e-50 171
MsG0680035289.01.T01 MTR_7g111700 39.252 321 134 11 10 315 50 324 3.25e-50 171
MsG0680035289.01.T01 MTR_5g072060 38.629 321 151 14 1 312 45 328 5.51e-50 175
MsG0680035289.01.T01 MTR_5g022850 38.871 319 154 12 13 315 19 312 6.60e-50 169
MsG0680035289.01.T01 MTR_6g079790 41.509 265 117 7 68 315 24 267 1.60e-49 167
MsG0680035289.01.T01 MTR_5g091140 38.580 324 153 11 1 315 87 373 3.98e-49 169
MsG0680035289.01.T01 MTR_5g074850 39.222 334 157 15 1 315 76 382 5.91e-49 175
MsG0680035289.01.T01 MTR_8g064890 37.895 285 146 6 34 313 86 344 8.70e-49 167
MsG0680035289.01.T01 MTR_5g064700 37.249 349 164 14 1 315 1 328 2.07e-48 166
MsG0680035289.01.T01 MTR_2g065840 37.143 315 149 10 14 314 65 344 3.48e-48 166
MsG0680035289.01.T01 MTR_2g066150 37.217 309 129 9 14 314 65 316 3.96e-48 165
MsG0680035289.01.T01 MTR_6g004450 42.478 226 101 6 92 314 27 226 7.53e-48 162
MsG0680035289.01.T01 MTR_8g046310 37.651 332 134 16 1 312 55 333 1.03e-47 164
MsG0680035289.01.T01 MTR_5g086590 39.496 238 107 7 81 315 14 217 3.59e-47 159
MsG0680035289.01.T01 MTR_5g024260 38.623 334 155 15 1 315 61 363 5.22e-47 163
MsG0680035289.01.T01 MTR_5g098950 41.026 234 89 4 82 315 90 274 1.08e-46 159
MsG0680035289.01.T01 MTR_5g075370 38.209 335 162 14 1 315 49 358 2.36e-46 167
MsG0680035289.01.T01 MTR_5g076490 38.730 315 146 12 1 303 3 282 3.60e-46 159
MsG0680035289.01.T01 MTR_5g097680 36.103 349 172 12 1 315 56 387 9.54e-46 160
MsG0680035289.01.T01 MTR_1g050312 38.542 288 115 12 34 312 16 250 1.71e-45 156
MsG0680035289.01.T01 MTR_1g053510 39.583 336 156 16 1 314 54 364 8.17e-45 157
MsG0680035289.01.T01 MTR_5g092850 37.584 298 148 12 34 315 83 358 2.06e-44 156
MsG0680035289.01.T01 MTR_2g021920 44.444 207 93 5 115 312 37 230 4.92e-44 152
MsG0680035289.01.T01 MTR_7g016930 40.304 263 117 11 41 293 77 309 2.45e-43 152
MsG0680035289.01.T01 MTR_6g087620 35.154 293 130 8 35 315 2 246 3.74e-43 150
MsG0680035289.01.T01 MTR_8g046200 37.539 317 140 13 36 315 85 380 4.95e-43 153
MsG0680035289.01.T01 MTR_5g023970 50.980 153 66 3 163 315 10 153 7.94e-43 146
MsG0680035289.01.T01 MTR_8g033140 33.041 342 178 11 1 315 58 375 3.09e-42 151
MsG0680035289.01.T01 MTR_8g032980 34.582 347 173 12 1 315 55 379 1.36e-41 149
MsG0680035289.01.T01 MTR_4g016000 39.462 223 99 7 85 306 2 189 6.09e-41 142
MsG0680035289.01.T01 MTR_8g046220 35.417 336 163 14 1 312 56 361 6.52e-41 147
MsG0680035289.01.T01 MTR_5g043605 38.462 260 118 9 63 315 74 298 1.96e-40 145
MsG0680035289.01.T01 MTR_8g036970 33.436 326 125 11 1 315 37 281 9.57e-40 143
MsG0680035289.01.T01 MTR_6g090260 47.712 153 72 2 164 315 75 220 9.98e-40 140
MsG0680035289.01.T01 MTR_8g033110 35.650 331 184 12 1 314 53 371 1.01e-39 144
MsG0680035289.01.T01 MTR_7g023800 35.152 330 138 11 1 315 1 269 2.41e-39 140
MsG0680035289.01.T01 MTR_8g033090 34.545 330 183 12 1 312 53 367 2.41e-38 140
MsG0680035289.01.T01 MTR_5g092860 33.537 328 168 12 1 315 41 331 2.99e-38 139
MsG0680035289.01.T01 MTR_6g009350 40.686 204 95 6 115 314 8 189 4.30e-38 134
MsG0680035289.01.T01 MTR_5g068490 34.799 273 133 9 43 314 8 236 4.50e-38 136
MsG0680035289.01.T01 MTR_8g063370 34.286 280 153 8 36 312 93 344 5.48e-38 139
MsG0680035289.01.T01 MTR_5g097850 47.651 149 69 3 169 315 63 204 1.20e-36 132
MsG0680035289.01.T01 MTR_4g014370 44.828 174 73 5 146 315 141 295 1.24e-36 134
MsG0680035289.01.T01 MTR_1g098180 35.077 325 137 14 1 312 58 321 1.63e-36 134
MsG0680035289.01.T01 MTR_7g019250 38.532 218 101 6 94 311 206 390 1.81e-36 136
MsG0680035289.01.T01 MTR_8g018110 35.542 332 160 14 1 311 53 351 2.98e-36 134
MsG0680035289.01.T01 MTR_8g033000 33.529 340 180 14 1 315 3 321 1.42e-35 132
MsG0680035289.01.T01 MTR_5g096900 36.424 302 131 13 1 280 51 313 2.76e-35 130
MsG0680035289.01.T01 MTR_2g104260 38.655 238 119 9 2 224 50 275 5.00e-35 128
MsG0680035289.01.T01 MTR_8g063350 33.904 292 142 12 34 312 45 298 3.82e-34 127
MsG0680035289.01.T01 MTR_3g018750 37.327 217 96 6 114 313 2 195 8.69e-34 124
MsG0680035289.01.T01 MTR_8g064790 32.753 287 115 8 33 315 83 295 3.77e-33 125
MsG0680035289.01.T01 MTR_5g097820 41.739 230 100 12 1 218 62 269 1.23e-32 122
MsG0680035289.01.T01 MTR_8g063310 32.653 294 151 12 34 312 89 350 4.88e-30 118
MsG0680035289.01.T01 MTR_7g104710 33.681 288 118 9 1 272 56 286 2.29e-29 114
MsG0680035289.01.T01 MTR_5g097540 39.869 153 75 4 164 315 97 233 3.02e-28 111
MsG0680035289.01.T01 MTR_5g066420 44.355 124 60 2 195 315 1 118 1.21e-27 105
MsG0680035289.01.T01 MTR_8g035690 35.268 224 105 9 91 295 38 240 2.87e-27 108
MsG0680035289.01.T01 MTR_7g101200 34.167 240 106 9 77 312 35 226 2.84e-26 104
MsG0680035289.01.T01 MTR_7g017150 43.023 172 79 6 139 307 61 216 2.50e-25 102
MsG0680035289.01.T01 MTR_7g017120 42.353 170 78 6 146 307 58 215 1.41e-24 100
MsG0680035289.01.T01 MTR_3g030620 45.185 135 68 3 180 311 5 136 1.70e-24 98.2
MsG0680035289.01.T01 MTR_5g094350 35.047 214 98 7 33 231 85 272 1.26e-23 98.2
MsG0680035289.01.T01 MTR_1g047080 34.454 238 111 12 1 231 3 202 1.82e-23 98.2
MsG0680035289.01.T01 MTR_1g050308 37.791 172 81 6 14 181 87 236 1.54e-22 96.7
MsG0680035289.01.T01 MTR_1g050308 48.611 72 33 2 243 312 236 305 7.05e-12 65.9
MsG0680035289.01.T01 MTR_1g076810 36.275 204 103 8 1 196 57 241 3.30e-22 97.4
MsG0680035289.01.T01 MTR_5g096920 43.443 122 56 3 195 315 1 110 6.04e-22 90.5
MsG0680035289.01.T01 MTR_6g070880 37.245 196 104 7 1 184 71 259 7.10e-22 93.2
MsG0680035289.01.T01 MTR_6g071420 37.245 196 104 7 1 184 71 259 7.10e-22 93.2
MsG0680035289.01.T01 MTR_6g079730 48.039 102 46 1 214 315 14 108 8.68e-22 90.1
MsG0680035289.01.T01 MTR_8g073570 43.651 126 65 5 1 125 49 169 1.15e-21 90.1
MsG0680035289.01.T01 MTR_5g037190 37.054 224 91 13 10 221 66 251 2.01e-20 89.0
MsG0680035289.01.T01 MTR_4g133170 26.953 256 135 8 69 309 93 311 6.22e-20 89.7
MsG0680035289.01.T01 MTR_5g086780 42.017 119 61 5 200 315 97 210 2.71e-18 82.8
MsG0680035289.01.T01 MTR_6g465360 37.433 187 95 10 1 171 101 281 3.56e-18 83.6
MsG0680035289.01.T01 MTR_5g097660 52.113 71 33 1 246 315 8 78 3.24e-17 76.6
MsG0680035289.01.T01 MTR_5g096050 41.441 111 48 2 195 295 1 104 3.35e-17 76.6
MsG0680035289.01.T01 MTR_4g094400 42.342 111 44 3 193 290 8 111 3.73e-17 77.0
MsG0680035289.01.T01 MTR_5g036250 70.833 48 14 0 268 315 23 70 3.67e-16 75.9
MsG0680035289.01.T01 MTR_2g022020 46.875 64 34 0 252 315 17 80 1.55e-15 72.0
MsG0680035289.01.T01 MTR_3g007430 25.941 239 135 10 69 302 137 338 4.13e-15 75.5
MsG0680035289.01.T01 MTR_2g026010 25.692 253 148 10 69 308 93 318 4.26e-15 75.5
MsG0680035289.01.T01 MTR_1g115205 33.721 172 86 5 37 202 87 236 3.69e-14 71.6
MsG0680035289.01.T01 MTR_2g024110 23.864 264 169 10 30 290 57 291 7.41e-14 72.0
MsG0680035289.01.T01 MTR_3g436150 31.111 225 120 11 69 290 137 329 7.93e-14 72.0
MsG0680035289.01.T01 MTR_1g097560 26.587 252 133 11 63 311 129 331 1.63e-13 70.9
MsG0680035289.01.T01 MTR_4g023080 27.801 241 113 9 80 310 117 306 3.71e-13 69.7
MsG0680035289.01.T01 MTR_0064s0160 28.185 259 147 13 63 311 149 378 7.47e-13 69.3
MsG0680035289.01.T01 MTR_5g048160 32.995 197 97 11 48 230 92 267 8.41e-13 68.9
MsG0680035289.01.T01 MTR_3g006670 28.194 227 121 11 69 292 149 336 1.34e-12 68.2
MsG0680035289.01.T01 MTR_3g014540 25.000 240 142 9 69 306 126 329 3.86e-12 66.6
MsG0680035289.01.T01 MTR_3g083640 29.825 228 98 13 69 289 159 331 3.89e-12 67.0
MsG0680035289.01.T01 MTR_4g021785 26.866 268 134 11 53 310 92 307 5.94e-12 66.2
MsG0680035289.01.T01 MTR_5g057790 26.695 236 121 10 69 297 145 335 6.40e-12 66.2
MsG0680035289.01.T01 MTR_4g023130 26.141 241 117 9 80 310 76 265 6.48e-12 65.5
MsG0680035289.01.T01 MTR_3g011060 28.000 225 126 9 69 292 135 324 6.87e-12 65.9
MsG0680035289.01.T01 MTR_3g047540 45.679 81 39 3 164 243 6 82 1.01e-11 60.8
MsG0680035289.01.T01 MTR_1g050305 44.304 79 33 3 245 312 2 80 1.64e-11 61.6
MsG0680035289.01.T01 MTR_2g031720 23.247 271 169 11 30 292 60 299 2.27e-11 64.3
MsG0680035289.01.T01 MTR_5g057710 26.939 245 109 11 69 297 145 335 3.43e-11 63.9
MsG0680035289.01.T01 MTR_1g068835 25.818 275 154 14 30 293 57 292 4.09e-11 63.5
MsG0680035289.01.T01 MTR_3g009240 26.222 225 122 10 69 290 131 314 4.92e-11 63.5
MsG0680035289.01.T01 MTR_3g007410 29.185 233 116 13 69 293 148 339 5.69e-11 63.2
MsG0680035289.01.T01 MTR_3g007360 28.632 234 116 12 69 293 148 339 7.02e-11 63.2
MsG0680035289.01.T01 MTR_3g007270 28.916 249 129 12 59 301 146 352 9.30e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 69 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAACCATCGACGAATTTA+AGG 0.207153 6:-101543300 MsG0680035289.01.T01:CDS
TGGACTAAACTCTTTATTCT+TGG 0.262083 6:-101542699 MsG0680035289.01.T01:CDS
CCGGGCATTCATCGTCCTAT+TGG 0.276259 6:-101542669 MsG0680035289.01.T01:CDS
TTATGATAAAGAAGCATATT+TGG 0.277040 6:-101542934 MsG0680035289.01.T01:CDS
CTCTTGGAGGAAACTTGATT+TGG 0.289577 6:-101543039 MsG0680035289.01.T01:CDS
CTTTATTCTTGGACCCTTAC+CGG 0.317936 6:-101542688 MsG0680035289.01.T01:CDS
ATCATGTGTCTTTGCCAATA+TGG 0.333166 6:-101543344 MsG0680035289.01.T01:CDS
GATTTAGTAGAGTCTGTTGT+AGG 0.344511 6:+101543498 None:intergenic
ATACAATATTTGTACCATAT+TGG 0.352124 6:+101543330 None:intergenic
TTTCATATCTGTTGCTTATG+AGG 0.367521 6:-101543540 MsG0680035289.01.T01:CDS
GGCTCTAACGGAGTAGGGCT+TGG 0.380768 6:+101543269 None:intergenic
CCATTCTTTCCCTCTCCAAT+AGG 0.382033 6:+101542654 None:intergenic
AAGAATGGGGATTTATTCTT+CGG 0.419637 6:-101542639 MsG0680035289.01.T01:CDS
ACCTTAAATTCGTCGATGGT+TGG 0.427797 6:+101543299 None:intergenic
ATATTGGGTCCCGTTATTAA+CGG 0.441956 6:-101543368 MsG0680035289.01.T01:CDS
ATGGTACAAATATTGTATTA+TGG 0.443096 6:-101543325 MsG0680035289.01.T01:CDS
ATAGCTTAAGAAATAACTCT+TGG 0.451447 6:-101543055 MsG0680035289.01.T01:CDS
TCTGAAGGTGATGATGAAAA+TGG 0.454622 6:-101543116 MsG0680035289.01.T01:CDS
TTTGGGTGAAGTTGGTGTAA+AGG 0.460837 6:-101542727 MsG0680035289.01.T01:CDS
CCTATTGGAGAGGGAAAGAA+TGG 0.462273 6:-101542654 MsG0680035289.01.T01:CDS
CACATGATTCCGTTAATAAC+GGG 0.476435 6:+101543359 None:intergenic
TTAGTAGAGTCTGTTGTAGG+AGG 0.484281 6:+101543501 None:intergenic
TCTAACGGAGTAGGGCTTGG+AGG 0.496217 6:+101543272 None:intergenic
ACACATGATTCCGTTAATAA+CGG 0.504481 6:+101543358 None:intergenic
TTTGGATATGCCTTGTGGTA+TGG 0.504658 6:-101543021 MsG0680035289.01.T01:CDS
ACTTGAATGGAGTGTGTCAT+TGG 0.506463 6:-101542974 MsG0680035289.01.T01:CDS
GCTTAAGAAATAACTCTTGG+AGG 0.509132 6:-101543052 MsG0680035289.01.T01:CDS
CTACTAAATCCATCAGATCA+TGG 0.509189 6:-101543485 MsG0680035289.01.T01:CDS
GGTGTCTATGTGTACTTGAA+TGG 0.510870 6:-101542987 MsG0680035289.01.T01:CDS
ACGTGAGATGAGACACTTGA+CGG 0.513236 6:-101542814 MsG0680035289.01.T01:CDS
CCCTACTCCGTTAGAGCCCT+CGG 0.514549 6:-101543264 MsG0680035289.01.T01:CDS
AACACATAACCATGATCTGA+TGG 0.517139 6:+101543476 None:intergenic
TTTATTCTTGGACCCTTACC+GGG 0.519024 6:-101542687 MsG0680035289.01.T01:CDS
GTGTGTCACGCGACGACGTG+TGG 0.519077 6:-101543085 MsG0680035289.01.T01:CDS
TAACGTACCGAGGGCTCTAA+CGG 0.520216 6:+101543257 None:intergenic
AAAATTATCAATCAAAGTTG+AGG 0.524428 6:+101542866 None:intergenic
AGGACGATGAATGCCCGGTA+AGG 0.524816 6:+101542674 None:intergenic
AAGTTGGTGTAAAGGAGTCT+TGG 0.532885 6:-101542719 MsG0680035289.01.T01:CDS
AAATTATCAATCAAAGTTGA+GGG 0.543167 6:+101542867 None:intergenic
ACGGAGTAGGGCTTGGAGGA+AGG 0.553604 6:+101543276 None:intergenic
GGACGATGAATGCCCGGTAA+GGG 0.555837 6:+101542675 None:intergenic
CCAATAGGACGATGAATGCC+CGG 0.555886 6:+101542669 None:intergenic
TTAATCTCTCTAAACATAGG+TGG 0.563672 6:+101543404 None:intergenic
CCGAGGGCTCTAACGGAGTA+GGG 0.571946 6:+101543264 None:intergenic
CAAAATTATAGTGACTCTGA+AGG 0.575610 6:-101543131 MsG0680035289.01.T01:CDS
GATCACATATTATAATCAGT+CGG 0.580068 6:-101542769 MsG0680035289.01.T01:CDS
ATCTCTCTAAACATAGGTGG+CGG 0.580164 6:+101543407 None:intergenic
GGTGTCATTTGACTTAAGCA+AGG 0.581015 6:-101542913 MsG0680035289.01.T01:CDS
TTTATTTGCTTTCTGGCGAG+AGG 0.590851 6:-101543454 MsG0680035289.01.T01:CDS
TGAATGGAGTGTGTCATTGG+TGG 0.597382 6:-101542971 MsG0680035289.01.T01:CDS
AAGGACCTTAAATTCGTCGA+TGG 0.607041 6:+101543295 None:intergenic
ACACGTCGTCGCGTGACACA+CGG 0.607304 6:+101543087 None:intergenic
ACGGGACCCAATATAAAACC+AGG 0.608292 6:+101543377 None:intergenic
ACCGAGGGCTCTAACGGAGT+AGG 0.609208 6:+101543263 None:intergenic
CTATTGGAGAGGGAAAGAAT+GGG 0.609846 6:-101542653 MsG0680035289.01.T01:CDS
TTCAATCTCATAACGTACCG+AGG 0.616467 6:+101543247 None:intergenic
ATTCATCGTCCTATTGGAGA+GGG 0.632527 6:-101542663 MsG0680035289.01.T01:CDS
CATTCATCGTCCTATTGGAG+AGG 0.635960 6:-101542664 MsG0680035289.01.T01:CDS
TCAATCTCATAACGTACCGA+GGG 0.639245 6:+101543248 None:intergenic
CGTTACAGTCATCTCTAACA+CGG 0.651494 6:+101543195 None:intergenic
CTTGATTTGGATATGCCTTG+TGG 0.655058 6:-101543026 MsG0680035289.01.T01:CDS
TCTGTTGCTTATGAGGACGA+TGG 0.655118 6:-101543533 MsG0680035289.01.T01:CDS
TGTGTCACGCGACGACGTGT+GGG 0.657396 6:-101543084 MsG0680035289.01.T01:CDS
CAACAGATATGAAACTGTTG+CGG 0.663097 6:+101543549 None:intergenic
ATAAAAGTCGCCATACCACA+AGG 0.667734 6:+101543011 None:intergenic
GTCATTTGACTTAAGCAAGG+AGG 0.684952 6:-101542910 MsG0680035289.01.T01:CDS
TATTGGAGAGGGAAAGAATG+GGG 0.686240 6:-101542652 MsG0680035289.01.T01:CDS
CGGGACCCAATATAAAACCA+GGG 0.700222 6:+101543378 None:intergenic
AATATGTGATCAAAGCAATG+TGG 0.717573 6:+101542781 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTATCAATCAAAGTTG+AGG + Chr6:101543320-101543339 None:intergenic 20.0%
!! AAATTATCAATCAAAGTTGA+GGG + Chr6:101543319-101543338 None:intergenic 20.0%
!! ATACAATATTTGTACCATAT+TGG + Chr6:101542856-101542875 None:intergenic 20.0%
!! ATGGTACAAATATTGTATTA+TGG - Chr6:101542858-101542877 MsG0680035289.01.T01:CDS 20.0%
!!! ACTTTTCACATATCAATTTT+GGG - Chr6:101543439-101543458 MsG0680035289.01.T01:CDS 20.0%
!!! TACTTTTCACATATCAATTT+TGG - Chr6:101543438-101543457 MsG0680035289.01.T01:CDS 20.0%
!!! TTATGATAAAGAAGCATATT+TGG - Chr6:101543249-101543268 MsG0680035289.01.T01:CDS 20.0%
!!! TTGATTGATAATTTTGATTC+TGG - Chr6:101543325-101543344 MsG0680035289.01.T01:CDS 20.0%
!!! TTTTTAATCTCTCTAAACAT+AGG + Chr6:101542785-101542804 None:intergenic 20.0%
! ATAGCTTAAGAAATAACTCT+TGG - Chr6:101543128-101543147 MsG0680035289.01.T01:CDS 25.0%
! GATCACATATTATAATCAGT+CGG - Chr6:101543414-101543433 MsG0680035289.01.T01:CDS 25.0%
!!! AAAAACCCTGGTTTTATATT+GGG - Chr6:101542800-101542819 MsG0680035289.01.T01:CDS 25.0%
!!! TAAAAACCCTGGTTTTATAT+TGG - Chr6:101542799-101542818 MsG0680035289.01.T01:CDS 25.0%
!!! TATGTGTTTTATTTGCTTTC+TGG - Chr6:101542722-101542741 MsG0680035289.01.T01:CDS 25.0%
AAGAATGGGGATTTATTCTT+CGG - Chr6:101543544-101543563 MsG0680035289.01.T01:CDS 30.0%
AATATGTGATCAAAGCAATG+TGG + Chr6:101543405-101543424 None:intergenic 30.0%
ACACATGATTCCGTTAATAA+CGG + Chr6:101542828-101542847 None:intergenic 30.0%
ATTGAACTGAAAAAGCTTCA+TGG - Chr6:101542953-101542972 MsG0680035289.01.T01:CDS 30.0%
CAAAATTATAGTGACTCTGA+AGG - Chr6:101543052-101543071 MsG0680035289.01.T01:CDS 30.0%
TGGACTAAACTCTTTATTCT+TGG - Chr6:101543484-101543503 MsG0680035289.01.T01:CDS 30.0%
TTAATCTCTCTAAACATAGG+TGG + Chr6:101542782-101542801 None:intergenic 30.0%
TTTAGAGAGATTAAAAACCC+TGG - Chr6:101542788-101542807 MsG0680035289.01.T01:CDS 30.0%
! TTTCATATCTGTTGCTTATG+AGG - Chr6:101542643-101542662 MsG0680035289.01.T01:CDS 30.0%
!! ATATCAATTTTGGGTGAAGT+TGG - Chr6:101543448-101543467 MsG0680035289.01.T01:CDS 30.0%
AACACATAACCATGATCTGA+TGG + Chr6:101542710-101542729 None:intergenic 35.0%
ATATTGGGTCCCGTTATTAA+CGG - Chr6:101542815-101542834 MsG0680035289.01.T01:CDS 35.0%
ATCATGTGTCTTTGCCAATA+TGG - Chr6:101542839-101542858 MsG0680035289.01.T01:CDS 35.0%
CAACAGATATGAAACTGTTG+CGG + Chr6:101542637-101542656 None:intergenic 35.0%
CACATGATTCCGTTAATAAC+GGG + Chr6:101542827-101542846 None:intergenic 35.0%
CTACTAAATCCATCAGATCA+TGG - Chr6:101542698-101542717 MsG0680035289.01.T01:CDS 35.0%
CTGAAAAAGCTTCATGGATT+TGG - Chr6:101542959-101542978 MsG0680035289.01.T01:CDS 35.0%
GATTTAGTAGAGTCTGTTGT+AGG + Chr6:101542688-101542707 None:intergenic 35.0%
GCTTAAGAAATAACTCTTGG+AGG - Chr6:101543131-101543150 MsG0680035289.01.T01:CDS 35.0%
! TCTGAAGGTGATGATGAAAA+TGG - Chr6:101543067-101543086 MsG0680035289.01.T01:CDS 35.0%
AAGGACCTTAAATTCGTCGA+TGG + Chr6:101542891-101542910 None:intergenic 40.0%
ACCTTAAATTCGTCGATGGT+TGG + Chr6:101542887-101542906 None:intergenic 40.0%
ACTTGAATGGAGTGTGTCAT+TGG - Chr6:101543209-101543228 MsG0680035289.01.T01:CDS 40.0%
ATAAAAGTCGCCATACCACA+AGG + Chr6:101543175-101543194 None:intergenic 40.0%
ATCTCTCTAAACATAGGTGG+CGG + Chr6:101542779-101542798 None:intergenic 40.0%
ATTCATCGTCCTATTGGAGA+GGG - Chr6:101543520-101543539 MsG0680035289.01.T01:CDS 40.0%
CGTTACAGTCATCTCTAACA+CGG + Chr6:101542991-101543010 None:intergenic 40.0%
CTATTGGAGAGGGAAAGAAT+GGG - Chr6:101543530-101543549 MsG0680035289.01.T01:CDS 40.0%
CTCTTGGAGGAAACTTGATT+TGG - Chr6:101543144-101543163 MsG0680035289.01.T01:CDS 40.0%
CTTTATTCTTGGACCCTTAC+CGG - Chr6:101543495-101543514 MsG0680035289.01.T01:CDS 40.0%
GGTGTCTATGTGTACTTGAA+TGG - Chr6:101543196-101543215 MsG0680035289.01.T01:CDS 40.0%
TATTGGAGAGGGAAAGAATG+GGG - Chr6:101543531-101543550 MsG0680035289.01.T01:CDS 40.0%
TCAATCTCATAACGTACCGA+GGG + Chr6:101542938-101542957 None:intergenic 40.0%
TCCAACCATCGACGAATTTA+AGG - Chr6:101542883-101542902 MsG0680035289.01.T01:CDS 40.0%
TTAGTAGAGTCTGTTGTAGG+AGG + Chr6:101542685-101542704 None:intergenic 40.0%
TTCAATCTCATAACGTACCG+AGG + Chr6:101542939-101542958 None:intergenic 40.0%
TTTATTCTTGGACCCTTACC+GGG - Chr6:101543496-101543515 MsG0680035289.01.T01:CDS 40.0%
TTTGGATATGCCTTGTGGTA+TGG - Chr6:101543162-101543181 MsG0680035289.01.T01:CDS 40.0%
! GTCATTTGACTTAAGCAAGG+AGG - Chr6:101543273-101543292 MsG0680035289.01.T01:CDS 40.0%
! TGTGGTATGGCGACTTTTAT+AGG - Chr6:101543175-101543194 MsG0680035289.01.T01:CDS 40.0%
! TTTATTTGCTTTCTGGCGAG+AGG - Chr6:101542729-101542748 MsG0680035289.01.T01:CDS 40.0%
!! AAGTTGGTGTAAAGGAGTCT+TGG - Chr6:101543464-101543483 MsG0680035289.01.T01:CDS 40.0%
!! CTTGATTTGGATATGCCTTG+TGG - Chr6:101543157-101543176 MsG0680035289.01.T01:CDS 40.0%
!! GGTGTCATTTGACTTAAGCA+AGG - Chr6:101543270-101543289 MsG0680035289.01.T01:CDS 40.0%
!! TTTGGGTGAAGTTGGTGTAA+AGG - Chr6:101543456-101543475 MsG0680035289.01.T01:CDS 40.0%
ACGGGACCCAATATAAAACC+AGG + Chr6:101542809-101542828 None:intergenic 45.0%
ACGTGAGATGAGACACTTGA+CGG - Chr6:101543369-101543388 MsG0680035289.01.T01:CDS 45.0%
CATTCATCGTCCTATTGGAG+AGG - Chr6:101543519-101543538 MsG0680035289.01.T01:CDS 45.0%
CCATTCTTTCCCTCTCCAAT+AGG + Chr6:101543532-101543551 None:intergenic 45.0%
CCTATTGGAGAGGGAAAGAA+TGG - Chr6:101543529-101543548 MsG0680035289.01.T01:CDS 45.0%
CGGGACCCAATATAAAACCA+GGG + Chr6:101542808-101542827 None:intergenic 45.0%
! TCTGTTGCTTATGAGGACGA+TGG - Chr6:101542650-101542669 MsG0680035289.01.T01:CDS 45.0%
! TGAATGGAGTGTGTCATTGG+TGG - Chr6:101543212-101543231 MsG0680035289.01.T01:CDS 45.0%
CCAATAGGACGATGAATGCC+CGG + Chr6:101543517-101543536 None:intergenic 50.0%
! TAACGTACCGAGGGCTCTAA+CGG + Chr6:101542929-101542948 None:intergenic 50.0%
AGGACGATGAATGCCCGGTA+AGG + Chr6:101543512-101543531 None:intergenic 55.0%
CCGGGCATTCATCGTCCTAT+TGG - Chr6:101543514-101543533 MsG0680035289.01.T01:CDS 55.0%
GGACGATGAATGCCCGGTAA+GGG + Chr6:101543511-101543530 None:intergenic 55.0%
TCTAACGGAGTAGGGCTTGG+AGG + Chr6:101542914-101542933 None:intergenic 55.0%
ACACGTCGTCGCGTGACACA+CGG + Chr6:101543099-101543118 None:intergenic 60.0%
ACGGAGTAGGGCTTGGAGGA+AGG + Chr6:101542910-101542929 None:intergenic 60.0%
CCCTACTCCGTTAGAGCCCT+CGG - Chr6:101542919-101542938 MsG0680035289.01.T01:CDS 60.0%
TGTGTCACGCGACGACGTGT+GGG - Chr6:101543099-101543118 MsG0680035289.01.T01:CDS 60.0%
! ACCGAGGGCTCTAACGGAGT+AGG + Chr6:101542923-101542942 None:intergenic 60.0%
! CCGAGGGCTCTAACGGAGTA+GGG + Chr6:101542922-101542941 None:intergenic 60.0%
! GGCTCTAACGGAGTAGGGCT+TGG + Chr6:101542917-101542936 None:intergenic 60.0%
GTGTGTCACGCGACGACGTG+TGG - Chr6:101543098-101543117 MsG0680035289.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr6 gene 101542629 101543576 101542629 ID=MsG0680035289.01;Name=MsG0680035289.01
Chr6 mRNA 101542629 101543576 101542629 ID=MsG0680035289.01.T01;Parent=MsG0680035289.01;Name=MsG0680035289.01.T01;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|315
Chr6 exon 101542629 101543576 101542629 ID=MsG0680035289.01.T01:exon:10758;Parent=MsG0680035289.01.T01
Chr6 CDS 101542629 101543576 101542629 ID=MsG0680035289.01.T01:cds;Parent=MsG0680035289.01.T01
Gene Sequence

>MsG0680035289.01.T01

ATGTACCGCAACAGTTTCATATCTGTTGCTTATGAGGACGATGGTTCATGTCTCCTCCTACAACAGACTCTACTAAATCCATCAGATCATGGTTATGTGTTTTATTTGCTTTCTGGCGAGAGGTTTGAGAAGAAAGTAAAATTATGTCCGCCACCTATGTTTAGAGAGATTAAAAACCCTGGTTTTATATTGGGTCCCGTTATTAACGGAATCATGTGTCTTTGCCAATATGGTACAAATATTGTATTATGGAATCCAACCATCGACGAATTTAAGGTCCTTCCTCCAAGCCCTACTCCGTTAGAGCCCTCGGTACGTTATGAGATTGAACTGAAAAAGCTTCATGGATTTGGTTATGACCGTGTTAGAGATGACTGTAACGTGATTAGATATGTACATTATGAATTAAATTTATCTAACTATCAAAATTATAGTGACTCTGAAGGTGATGATGAAAATGGTAGTTACCGTGTGTCACGCGACGACGTGTGGGAGATATATAGCTTAAGAAATAACTCTTGGAGGAAACTTGATTTGGATATGCCTTGTGGTATGGCGACTTTTATAGGTGTCTATGTGTACTTGAATGGAGTGTGTCATTGGTGGACTTATGATGATGATTATGATAAAGAAGCATATTTGGTGTCATTTGACTTAAGCAAGGAGGTGTTTTGTAAAACACTCATGCCCTCAACTTTGATTGATAATTTTGATTCTGGATGTGCGATGAGACGCTTTAGACGTGAGATGAGACACTTGACGGTGTTCAATGACCACATTGCTTTGATCACATATTATAATCAGTCGGCTACTTTTCACATATCAATTTTGGGTGAAGTTGGTGTAAAGGAGTCTTGGACTAAACTCTTTATTCTTGGACCCTTACCGGGCATTCATCGTCCTATTGGAGAGGGAAAGAATGGGGATTTATTCTTCGGAAGAAAATGA
Protein sequence

>MsG0680035289.01.T01

MYRNSFISVAYEDDGSCLLLQQTLLNPSDHGYVFYLLSGERFEKKVKLCPPPMFREIKNPGFILGPVINGIMCLCQYGTNIVLWNPTIDEFKVLPPSPTPLEPSVRYEIELKKLHGFGYDRVRDDCNVIRYVHYELNLSNYQNYSDSEGDDENGSYRVSRDDVWEIYSLRNNSWRKLDLDMPCGMATFIGVYVYLNGVCHWWTYDDDYDKEAYLVSFDLSKEVFCKTLMPSTLIDNFDSGCAMRRFRREMRHLTVFNDHIALITYYNQSATFHISILGEVGVKESWTKLFILGPLPGIHRPIGEGKNGDLFFGRK*