Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012220.01.T01 | XP_003617550.1 | 71.066 | 394 | 93 | 8 | 1 | 387 | 1 | 380 | 7.54E-180 | 525 |
| MsG0380012220.01.T01 | XP_003617550.1 | 69.668 | 211 | 53 | 4 | 413 | 620 | 198 | 400 | 5.61E-83 | 277 |
Co-expression Network
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012220.01.T01 | MTR_5g092850 | 71.066 | 394 | 93 | 8 | 1 | 387 | 1 | 380 | 0.0 | 525 |
| MsG0380012220.01.T01 | MTR_5g092850 | 69.668 | 211 | 53 | 4 | 413 | 620 | 198 | 400 | 6.80e-87 | 277 |
| MsG0380012220.01.T01 | MTR_5g074850 | 61.779 | 416 | 134 | 9 | 15 | 421 | 34 | 433 | 5.52e-165 | 496 |
| MsG0380012220.01.T01 | MTR_5g074850 | 63.902 | 205 | 59 | 5 | 415 | 613 | 222 | 417 | 4.52e-73 | 253 |
| MsG0380012220.01.T01 | MTR_5g075370 | 64.230 | 383 | 114 | 9 | 14 | 384 | 6 | 377 | 6.95e-158 | 473 |
| MsG0380012220.01.T01 | MTR_5g075370 | 63.500 | 200 | 58 | 5 | 415 | 608 | 198 | 388 | 2.08e-70 | 243 |
| MsG0380012220.01.T01 | MTR_3g018750 | 87.773 | 229 | 24 | 2 | 165 | 393 | 1 | 225 | 2.45e-140 | 408 |
| MsG0380012220.01.T01 | MTR_3g018750 | 90.865 | 208 | 18 | 1 | 415 | 622 | 35 | 241 | 1.11e-132 | 389 |
| MsG0380012220.01.T01 | MTR_5g097220 | 45.669 | 381 | 187 | 11 | 16 | 385 | 13 | 384 | 6.60e-89 | 282 |
| MsG0380012220.01.T01 | MTR_5g097220 | 47.087 | 206 | 99 | 4 | 418 | 622 | 212 | 408 | 1.19e-45 | 167 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012220.01.T01 | AT4G12560 | 26.289 | 388 | 231 | 16 | 19 | 394 | 7 | 351 | 5.03e-21 | 96.3 |
| MsG0380012220.01.T01 | AT4G12560 | 26.289 | 388 | 231 | 16 | 19 | 394 | 7 | 351 | 5.03e-21 | 96.3 |
| MsG0380012220.01.T01 | AT4G12560 | 26.289 | 388 | 231 | 16 | 19 | 394 | 7 | 351 | 5.03e-21 | 96.3 |
| MsG0380012220.01.T01 | AT4G22390 | 24.051 | 316 | 194 | 13 | 92 | 398 | 74 | 352 | 7.18e-12 | 68.2 |
| MsG0380012220.01.T01 | AT4G22390 | 24.051 | 316 | 194 | 13 | 92 | 398 | 74 | 352 | 8.10e-12 | 68.2 |
| MsG0380012220.01.T01 | AT2G40920 | 23.250 | 400 | 225 | 17 | 19 | 385 | 57 | 407 | 4.02e-11 | 65.9 |
| MsG0380012220.01.T01 | AT2G40920 | 23.291 | 395 | 221 | 17 | 24 | 385 | 3 | 348 | 5.86e-11 | 65.1 |
| MsG0380012220.01.T01 | AT2G40925 | 21.481 | 405 | 250 | 16 | 16 | 397 | 27 | 386 | 6.04e-11 | 65.1 |
| MsG0380012220.01.T01 | AT3G07870 | 25.070 | 359 | 215 | 14 | 18 | 359 | 30 | 351 | 8.17e-11 | 65.1 |
Find 99 sgRNAs with CRISPR-Local
Find 190 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATCTGCCAAAAGGGTTGA+AGG | 0.449446 | 3:-12888820 | None:intergenic |
| AAATCTGCCAAAAGGGTTTA+AGG | 0.147956 | 3:-12890791 | None:intergenic |
| AACAACGTAGCTAGAGCGCA+AGG | 0.635054 | 3:-12891188 | None:intergenic |
| AAGTTGGTGTGAAAGAATCA+TGG | 0.567256 | 3:+12891141 | MsG0380012220.01.T01:CDS |
| AATCGTTTCAAAGATTTAAT+CGG | 0.291137 | 3:-12888272 | None:intergenic |
| AATCTGCCAAAAGGGTTGAA+GGG | 0.564047 | 3:-12888819 | None:intergenic |
| AATCTGCCAAAAGGGTTTAA+GGG | 0.371094 | 3:-12890790 | None:intergenic |
| AATGGCTTTGCGAGTGTTAA+TGG | 0.242296 | 3:+12888515 | MsG0380012220.01.T01:CDS |
| AATGTCACTGCTACAGAAAT+TGG | 0.311940 | 3:-12888195 | None:intergenic |
| AATTTCCGAGTGTATATGAA+TGG | 0.524704 | 3:+12888926 | MsG0380012220.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACAAAGAAAAGGGAAGTTA+TGG | - | Chr3:12889998-12890017 | None:intergenic | 30.0% | |
| AACTACAATGAAGAATATGC+AGG | - | Chr3:12889489-12889508 | None:intergenic | 30.0% | |
| AACTTGAGTGAAAATCCTAA+AGG | - | Chr3:12890513-12890532 | None:intergenic | 30.0% | |
| AATTTCCGAGTGTATATGAA+TGG | + | Chr3:12888926-12888945 | MsG0380012220.01.T01:CDS | 30.0% | |
| ACTACAAAAGTTTCTCACTA+TGG | + | Chr3:12888555-12888574 | MsG0380012220.01.T01:CDS | 30.0% | |
| ACTTGAGTGAAAATCCTAAA+GGG | - | Chr3:12890512-12890531 | None:intergenic | 30.0% | |
| AGAATATCAATCTGCTAGAA+TGG | - | Chr3:12890434-12890453 | None:intergenic | 30.0% | |
| AGTAAGAGATTCAAATGAAC+CGG | - | Chr3:12890248-12890267 | None:intergenic | 30.0% | |
| ATAAATTACCACAACTCAGA+AGG | - | Chr3:12889836-12889855 | None:intergenic | 30.0% | |
| ATACATGCTTCTGCAAAAAA+AGG | - | Chr3:12888995-12889014 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 12888188 | 12891391 | 12888188 | ID=MsG0380012220.01;Name=MsG0380012220.01 |
| Chr3 | mRNA | 12888188 | 12891391 | 12888188 | ID=MsG0380012220.01.T01;Parent=MsG0380012220.01;Name=MsG0380012220.01.T01;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|622 |
| Chr3 | exon | 12888188 | 12889364 | 12888188 | ID=MsG0380012220.01.T01:exon:412;Parent=MsG0380012220.01.T01 |
| Chr3 | exon | 12890700 | 12891391 | 12890700 | ID=MsG0380012220.01.T01:exon:413;Parent=MsG0380012220.01.T01 |
| Chr3 | CDS | 12888188 | 12889364 | 12888188 | ID=MsG0380012220.01.T01:cds;Parent=MsG0380012220.01.T01 |
| Chr3 | CDS | 12890700 | 12891391 | 12890700 | ID=MsG0380012220.01.T01:cds;Parent=MsG0380012220.01.T01 |
| Gene Sequence |
>MsG0380012220.01.T01 ATGGCTTCCAATTTCTGTAGCAGTGACATTGTAAGTGGAGGCTATATTCACAATGATATTGCCTTCTTTATTCTATCAAAGCTCCCGATTAAATCTTTGAAACGATTTGAATGCGTAAGTAAATCATGGTCTCGCTTTACTGAAGACTCTCTTTTCATGACAATGTATCGCAAAAATCTTCTTTTATCTCAATCTTATGACGGTGATACATCTCTCCTCGTAAATATCTGCTCTGACAGTAAAAAGTTCCATTTTCTATCAGGTGAAAGGTTTGAGAATTGGGTCAGTTTAATTTGTCCAGTTAAATCCTATCGTGCAGTTGAAATCAATGGCTTTGCGAGTGTTAATGGCATACTTTGTTTGAAACACTACAAAAGTTTCTCACTATGGAATCCAACTACGGACGAAGTCAAGGTTATTCCTCCTTCTGGGACACTTCTTCCACCTATTGTTCATAAATATAAGGCGGCGGATTACTTTCACCATCGCACTATGATTCATGGATTTGGTTATGACTCTATTGCTGATGACTATAAGGTGATTTGTCTTGAAACATTTGAATCATTATTCTTTAGTGATGATGAGCTAGCTATAGGCAAGAAATTACAATCATTGTTGTTGCAAGATGGATCCCTTCAACCCTTTTGGCAGATTTATAGCCTAAAAAGTAACTCGTGGAAGAAACTCTATGTCAATATGCCTCATTCTTCAACTTTGCTCGTGGCCGGTTATTATGGAAATTTCCGAGTGTATATGAATGGAGCGTGTCATTGGTTGTCTATGCCTCGTTGTCATTGGACTGTTCCTTTTTTTGCAGAAGCATGTATGGTATCATTTGACTTAAACAATGAGACATTCTTTGTCACACCCATACCATCGTACGTTAGACTGACATGGACACACTTGACAGTGTTGAATAATTCCATTGCCTTGATCTTCTTCTCTGTGAACTCAAATATTTTTCACATATCAATTTTGGGTGAATTTGGTGTGAAGGAATCATGGATCAAACTGTTCACTGTTGAAATACCTTGCGCTCTAGCTTCGTTTCCTATGGGGGTCGTTATGAATGGTGAAATAGTCTTTGCAAATGACGACAACAAACTACTTTTGTTTGATTTAAATACCAACAAGATTGTAGAGCTTGGACTTAAAATAAGAGATAGATTGACTCTTGGTATTTCATGCTCAAATTGTTTGGAACTCTTTTGTTGTTGCAATCATTGTTGTTGCAAGAAATTACAATCATTGTTGTTGCAAGATGAATCCCTTAAACCCTTTTGGCAGATTTATAGCCTAAAAAGTAACTCGTGGAAGAAACTCAATGTCAATATGCCTCATTCTTCAACTTTGCTCGTGGCCAGTTTGCATGGAAATTTCCGAGTGTATATGAATGGAGTGTGTCATTGGTTGTCTATGCCTCGTTGTCATTGGACTGTTCCTTTTTCTGCAGAAGCATGTATGGTATCATTTGACTTAAACAATGAGACGTTCTTTGTCACACCCCTACCCTCGTACGTTAGACTGACATGGACACAATTGACAGTGTTGAATAATTCCATTGCCTTGATCTTCTTCTCTATAATCACAAATATTTTTCACATATCAATTTTGGGTGAAGTTGGTGTGAAAGAATCATGGATCAAACTCTTCACTATTGAAAAACCTTGCGCTCTAGCTACGTTGTTTCCTATGGGAGTCGGTATGAATGGTGAAATAGTCTTTGCAAATGACGACAACAAACTACTTTTGTTTGATTTAAATACCAACAAGATTGTAGAGCTTGGACTTAAAATAAGAGATAAATTGACTCTTGGTCAGATTAAAGTTTACAAGAGAAGCCTTCTTCCAATCAAAAGAATAAATTAA |
| Protein sequence |
>MsG0380012220.01.T01 MASNFCSSDIVSGGYIHNDIAFFILSKLPIKSLKRFECVSKSWSRFTEDSLFMTMYRKNLLLSQSYDGDTSLLVNICSDSKKFHFLSGERFENWVSLICPVKSYRAVEINGFASVNGILCLKHYKSFSLWNPTTDEVKVIPPSGTLLPPIVHKYKAADYFHHRTMIHGFGYDSIADDYKVICLETFESLFFSDDELAIGKKLQSLLLQDGSLQPFWQIYSLKSNSWKKLYVNMPHSSTLLVAGYYGNFRVYMNGACHWLSMPRCHWTVPFFAEACMVSFDLNNETFFVTPIPSYVRLTWTHLTVLNNSIALIFFSVNSNIFHISILGEFGVKESWIKLFTVEIPCALASFPMGVVMNGEIVFANDDNKLLLFDLNTNKIVELGLKIRDRLTLGISCSNCLELFCCCNHCCCKKLQSLLLQDESLKPFWQIYSLKSNSWKKLNVNMPHSSTLLVASLHGNFRVYMNGVCHWLSMPRCHWTVPFSAEACMVSFDLNNETFFVTPLPSYVRLTWTQLTVLNNSIALIFFSIITNIFHISILGEVGVKESWIKLFTIEKPCALATLFPMGVGMNGEIVFANDDNKLLLFDLNTNKIVELGLKIRDKLTLGQIKVYKRSLLPIKRIN* |
