AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018234.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018234.01.T01 MTR_4g007940 85.714 343 46 2 9 349 1 342 0.0 596
MsG0480018234.01.T01 MTR_0831s0010 59.599 349 124 3 1 348 89 421 1.94e-138 400
MsG0480018234.01.T01 MTR_8g063330 66.558 308 92 4 1 306 53 351 1.90e-132 386
MsG0480018234.01.T01 MTR_4g016000 72.038 211 37 2 88 298 2 190 9.22e-104 304
MsG0480018234.01.T01 MTR_0129s0070 42.061 359 183 5 4 349 51 397 8.07e-90 275
MsG0480018234.01.T01 MTR_8g063440 45.480 354 168 8 1 349 51 384 9.62e-90 274
MsG0480018234.01.T01 MTR_0129s0120 42.416 356 180 5 5 347 52 395 1.97e-88 271
MsG0480018234.01.T01 MTR_2g066170 44.293 368 155 14 1 349 51 387 6.22e-83 257
MsG0480018234.01.T01 MTR_7g019250 59.914 232 66 4 97 325 206 413 2.29e-81 256
MsG0480018234.01.T01 MTR_2g021800 40.526 380 178 11 1 349 55 417 3.72e-81 253
MsG0480018234.01.T01 MTR_8g464770 41.449 345 176 8 2 342 52 374 2.67e-79 247
MsG0480018234.01.T01 MTR_2g022000 40.782 358 167 11 1 328 55 397 6.46e-79 248
MsG0480018234.01.T01 MTR_2g066200 41.758 364 172 11 1 349 51 389 5.50e-77 242
MsG0480018234.01.T01 MTR_3g011120 44.282 341 158 9 2 336 54 368 9.50e-76 238
MsG0480018234.01.T01 MTR_5g097330 41.257 366 178 13 1 347 55 402 1.57e-75 238
MsG0480018234.01.T01 MTR_4g117550 40.449 356 168 7 1 349 56 374 7.26e-75 236
MsG0480018234.01.T01 MTR_2g038680 39.837 369 177 13 1 342 55 405 9.67e-74 234
MsG0480018234.01.T01 MTR_4g102410 40.111 359 184 10 1 342 100 444 1.42e-73 234
MsG0480018234.01.T01 MTR_5g097350 38.827 358 180 10 1 346 55 385 1.29e-72 230
MsG0480018234.01.T01 MTR_6g086825 39.024 369 189 8 1 349 109 461 1.39e-72 233
MsG0480018234.01.T01 MTR_2g104340 40.720 361 174 13 2 342 50 390 4.00e-71 227
MsG0480018234.01.T01 MTR_2g065860 41.257 366 171 11 1 349 51 389 4.93e-71 226
MsG0480018234.01.T01 MTR_0129s0050 40.108 369 175 12 1 342 53 402 1.41e-70 226
MsG0480018234.01.T01 MTR_2g021770 38.251 366 187 11 1 342 51 401 5.18e-70 224
MsG0480018234.01.T01 MTR_8g467670 39.106 358 179 10 1 349 53 380 1.04e-68 221
MsG0480018234.01.T01 MTR_6g080680 37.912 364 172 10 1 342 58 389 1.35e-68 220
MsG0480018234.01.T01 MTR_5g097220 40.157 381 169 11 1 347 52 407 1.51e-68 221
MsG0480018234.01.T01 MTR_8g041270 39.782 367 178 14 2 342 51 400 2.26e-68 220
MsG0480018234.01.T01 MTR_8g064890 41.379 319 155 8 34 341 84 381 2.41e-68 219
MsG0480018234.01.T01 MTR_2g021950 37.921 356 188 11 1 342 55 391 4.08e-68 219
MsG0480018234.01.T01 MTR_2g034700 41.436 362 157 13 1 348 53 373 1.37e-67 217
MsG0480018234.01.T01 MTR_1g007450 40.173 346 179 9 1 333 51 381 2.30e-66 215
MsG0480018234.01.T01 MTR_5g074800 41.916 334 169 12 1 323 1 320 4.32e-66 212
MsG0480018234.01.T01 MTR_4g014370 37.337 383 170 12 1 342 50 403 1.88e-65 213
MsG0480018234.01.T01 MTR_5g069390 39.402 368 180 13 1 344 65 413 2.70e-65 214
MsG0480018234.01.T01 MTR_7g079640 39.521 334 163 11 1 326 52 354 3.74e-65 211
MsG0480018234.01.T01 MTR_2g066150 38.674 362 158 10 1 349 51 361 4.25e-65 210
MsG0480018234.01.T01 MTR_5g096630 38.251 366 188 10 1 342 57 408 5.72e-65 211
MsG0480018234.01.T01 MTR_8g039500 42.378 328 146 12 1 317 51 346 6.55e-65 209
MsG0480018234.01.T01 MTR_2g065840 41.279 344 155 11 1 326 51 365 7.90e-65 210
MsG0480018234.01.T01 MTR_6g078010 38.674 362 167 10 1 342 3 329 8.67e-65 209
MsG0480018234.01.T01 MTR_1g050312 43.226 310 126 12 29 329 8 276 1.71e-64 206
MsG0480018234.01.T01 MTR_4g055820 41.066 319 159 10 2 304 54 359 2.54e-64 208
MsG0480018234.01.T01 MTR_0054s0100 38.138 333 175 8 36 349 89 409 2.65e-64 209
MsG0480018234.01.T01 MTR_8g012090 38.554 332 137 8 1 317 55 334 3.20e-64 207
MsG0480018234.01.T01 MTR_5g037680 38.462 364 172 15 1 342 47 380 6.71e-64 210
MsG0480018234.01.T01 MTR_6g090180 38.017 363 188 13 1 342 86 432 1.41e-63 208
MsG0480018234.01.T01 MTR_0054s0090 38.554 332 173 8 36 349 89 407 3.10e-63 207
MsG0480018234.01.T01 MTR_7g069550 39.769 347 177 12 1 331 53 383 3.62e-63 206
MsG0480018234.01.T01 MTR_4g130330 37.637 364 173 13 3 339 45 381 4.26e-63 206
MsG0480018234.01.T01 MTR_5g064700 37.831 378 183 14 1 342 1 362 1.05e-62 204
MsG0480018234.01.T01 MTR_5g024260 41.210 347 160 13 1 326 61 384 1.37e-62 205
MsG0480018234.01.T01 MTR_6g079810 40.361 332 160 10 36 342 139 457 1.56e-62 206
MsG0480018234.01.T01 MTR_7g017130 37.394 353 192 9 1 342 57 391 2.32e-62 204
MsG0480018234.01.T01 MTR_5g097680 37.240 384 181 11 1 342 56 421 9.87e-61 201
MsG0480018234.01.T01 MTR_7g017170 37.500 336 181 9 1 325 57 374 1.26e-60 200
MsG0480018234.01.T01 MTR_4g028510 37.363 364 183 13 1 342 56 396 3.04e-60 200
MsG0480018234.01.T01 MTR_1g007410 38.187 364 188 13 1 348 61 403 3.79e-60 199
MsG0480018234.01.T01 MTR_5g043605 39.557 316 146 8 46 347 54 338 4.17e-60 197
MsG0480018234.01.T01 MTR_6g478230 36.290 372 185 10 1 349 66 408 4.79e-60 199
MsG0480018234.01.T01 MTR_0134s0060 35.753 372 191 11 2 342 52 406 8.71e-60 198
MsG0480018234.01.T01 MTR_8g033140 35.567 388 185 12 1 349 58 419 1.22e-59 197
MsG0480018234.01.T01 MTR_7g017040 37.466 363 181 11 1 342 55 392 2.05e-59 196
MsG0480018234.01.T01 MTR_6g090250 36.338 355 168 9 36 346 82 422 3.72e-59 196
MsG0480018234.01.T01 MTR_5g059430 37.401 377 184 12 1 344 65 422 4.81e-59 196
MsG0480018234.01.T01 MTR_8g036970 38.187 364 138 12 1 346 37 331 4.81e-59 195
MsG0480018234.01.T01 MTR_2g104280 38.904 365 184 15 2 342 50 399 5.53e-59 196
MsG0480018234.01.T01 MTR_8g013000 41.066 319 159 11 1 305 49 352 3.39e-58 194
MsG0480018234.01.T01 MTR_6g079770 36.193 373 190 13 1 342 56 411 4.28e-58 194
MsG0480018234.01.T01 MTR_4g028660 38.188 309 155 10 36 326 87 377 1.35e-57 192
MsG0480018234.01.T01 MTR_4g027450 38.188 309 155 10 36 326 87 377 1.35e-57 192
MsG0480018234.01.T01 MTR_8g032980 37.018 389 184 13 1 349 55 422 1.61e-57 192
MsG0480018234.01.T01 MTR_8g046200 36.709 395 172 13 5 346 51 420 2.24e-57 192
MsG0480018234.01.T01 MTR_3g019290 59.748 159 28 1 1 159 53 175 5.01e-57 183
MsG0480018234.01.T01 MTR_4g028680 37.297 370 188 15 1 349 55 401 1.30e-56 189
MsG0480018234.01.T01 MTR_7g111700 37.808 365 166 12 5 349 43 366 1.89e-56 188
MsG0480018234.01.T01 MTR_1g098180 36.695 357 161 12 1 344 58 362 8.79e-56 186
MsG0480018234.01.T01 MTR_4g028750 37.419 310 155 11 36 326 87 376 4.73e-55 185
MsG0480018234.01.T01 MTR_6g461660 36.712 365 188 14 1 346 58 398 1.55e-54 184
MsG0480018234.01.T01 MTR_8g046310 38.122 362 168 17 1 346 55 376 3.62e-54 182
MsG0480018234.01.T01 MTR_5g068490 38.511 309 148 9 45 349 8 278 4.82e-54 179
MsG0480018234.01.T01 MTR_5g072060 39.763 337 155 12 1 323 45 347 5.60e-54 187
MsG0480018234.01.T01 MTR_1g053510 36.000 375 183 14 1 344 54 402 2.18e-53 181
MsG0480018234.01.T01 MTR_2g064950 35.429 350 173 13 1 326 49 369 3.65e-53 180
MsG0480018234.01.T01 MTR_5g096910 36.858 331 155 14 35 342 88 387 4.48e-53 180
MsG0480018234.01.T01 MTR_6g079790 36.102 313 166 9 58 349 11 310 7.75e-53 177
MsG0480018234.01.T01 MTR_5g091140 37.500 368 175 16 1 349 87 418 1.05e-52 179
MsG0480018234.01.T01 MTR_6g004450 42.797 236 110 5 113 342 47 263 1.20e-51 172
MsG0480018234.01.T01 MTR_5g022850 35.583 326 174 9 35 342 40 347 1.77e-51 174
MsG0480018234.01.T01 MTR_2g021920 39.062 256 121 7 103 342 31 267 2.02e-51 172
MsG0480018234.01.T01 MTR_8g046220 35.079 382 179 13 1 346 56 404 2.85e-50 173
MsG0480018234.01.T01 MTR_5g086590 41.767 249 119 8 84 330 14 238 7.32e-50 167
MsG0480018234.01.T01 MTR_8g033110 33.773 379 202 13 1 347 53 414 9.26e-50 172
MsG0480018234.01.T01 MTR_8g033000 34.211 380 191 11 1 344 3 359 1.52e-49 170
MsG0480018234.01.T01 MTR_7g023800 32.773 357 167 10 1 339 1 302 2.13e-49 168
MsG0480018234.01.T01 MTR_5g092860 34.278 353 161 13 1 325 41 350 2.87e-49 169
MsG0480018234.01.T01 MTR_6g087620 34.853 307 143 5 36 326 1 266 5.04e-49 166
MsG0480018234.01.T01 MTR_6g090260 44.059 202 92 4 154 346 73 262 1.59e-48 164
MsG0480018234.01.T01 MTR_8g063350 36.624 314 144 9 36 326 45 326 1.93e-48 166
MsG0480018234.01.T01 MTR_8g063310 35.430 302 163 11 36 323 89 372 5.50e-48 167
MsG0480018234.01.T01 MTR_1g007430 37.342 316 160 9 1 305 35 323 6.34e-48 164
MsG0480018234.01.T01 MTR_5g092850 35.072 345 173 13 31 346 78 400 2.60e-47 165
MsG0480018234.01.T01 MTR_5g076490 38.720 328 152 12 36 342 31 330 3.22e-47 163
MsG0480018234.01.T01 MTR_5g023970 45.226 199 83 5 145 336 4 183 4.47e-47 158
MsG0480018234.01.T01 MTR_8g064790 34.294 347 152 10 1 341 54 330 2.26e-46 161
MsG0480018234.01.T01 MTR_8g063370 34.503 342 176 11 1 326 63 372 2.94e-45 159
MsG0480018234.01.T01 MTR_4g014370 45.178 197 79 5 156 342 154 331 4.07e-45 157
MsG0480018234.01.T01 MTR_5g074850 32.676 355 186 14 1 328 76 404 1.61e-44 164
MsG0480018234.01.T01 MTR_5g097850 35.034 294 124 9 57 342 5 239 3.12e-44 153
MsG0480018234.01.T01 MTR_8g035690 37.075 294 129 10 89 347 30 302 3.95e-44 154
MsG0480018234.01.T01 MTR_8g033090 33.333 378 202 14 1 347 53 411 1.23e-43 155
MsG0480018234.01.T01 MTR_8g018110 31.398 379 193 13 1 347 53 396 1.22e-41 150
MsG0480018234.01.T01 MTR_7g016930 37.193 285 138 8 1 279 55 304 2.25e-41 147
MsG0480018234.01.T01 MTR_3g018750 36.047 258 122 10 114 347 2 240 1.55e-40 143
MsG0480018234.01.T01 MTR_5g075370 31.667 360 186 16 1 328 49 380 2.22e-39 148
MsG0480018234.01.T01 MTR_5g098950 38.053 226 93 6 85 305 90 273 2.78e-39 140
MsG0480018234.01.T01 MTR_1g050308 33.837 331 137 9 1 325 73 327 4.33e-39 142
MsG0480018234.01.T01 MTR_4g106530 48.921 139 23 1 94 232 1 91 1.19e-37 130
MsG0480018234.01.T01 MTR_6g009350 38.318 214 89 7 115 311 8 195 1.08e-35 129
MsG0480018234.01.T01 MTR_3g030620 42.690 171 86 4 176 343 16 177 8.64e-34 123
MsG0480018234.01.T01 MTR_6g079730 44.218 147 70 1 200 346 14 148 3.45e-33 120
MsG0480018234.01.T01 MTR_5g066420 40.881 159 78 3 183 336 1 148 3.88e-32 118
MsG0480018234.01.T01 MTR_5g096900 34.122 296 137 12 1 271 51 313 9.77e-31 119
MsG0480018234.01.T01 MTR_5g097540 43.421 152 70 4 155 305 96 232 1.68e-30 118
MsG0480018234.01.T01 MTR_5g036250 57.732 97 40 1 244 340 8 103 2.65e-30 114
MsG0480018234.01.T01 MTR_5g096920 38.509 161 83 4 183 342 1 146 3.40e-28 107
MsG0480018234.01.T01 MTR_5g097660 53.846 104 47 1 244 346 15 118 4.42e-28 106
MsG0480018234.01.T01 MTR_2g022020 45.312 128 56 2 215 342 1 114 7.47e-28 105
MsG0480018234.01.T01 MTR_5g094350 35.749 207 90 6 36 218 86 273 5.25e-27 108
MsG0480018234.01.T01 MTR_2g104260 35.776 232 124 11 2 212 50 277 5.88e-27 107
MsG0480018234.01.T01 MTR_7g104710 31.541 279 127 13 1 263 56 286 1.65e-26 107
MsG0480018234.01.T01 MTR_5g097820 33.180 217 123 7 1 204 62 269 2.27e-26 106
MsG0480018234.01.T01 MTR_7g017150 37.186 199 106 5 155 347 81 266 1.34e-25 103
MsG0480018234.01.T01 MTR_7g101200 33.191 235 99 9 84 305 39 228 1.43e-25 103
MsG0480018234.01.T01 MTR_7g017120 36.683 199 107 5 155 347 80 265 6.68e-25 102
MsG0480018234.01.T01 MTR_1g047080 28.205 351 171 13 1 344 3 279 1.70e-22 95.9
MsG0480018234.01.T01 MTR_5g086780 53.086 81 38 0 244 324 148 228 1.70e-22 94.7
MsG0480018234.01.T01 MTR_1g050305 54.167 96 35 3 240 326 8 103 8.55e-22 90.5
MsG0480018234.01.T01 MTR_1g076810 34.010 197 105 8 1 184 57 241 9.70e-22 96.7
MsG0480018234.01.T01 MTR_6g070880 35.052 194 99 8 1 174 71 257 2.93e-20 89.4
MsG0480018234.01.T01 MTR_6g071420 35.052 194 99 8 1 174 71 257 2.93e-20 89.4
MsG0480018234.01.T01 MTR_1g115205 36.875 160 85 6 39 191 87 237 1.36e-19 87.4
MsG0480018234.01.T01 MTR_4g133170 23.262 374 214 14 4 349 38 366 2.91e-19 88.6
MsG0480018234.01.T01 MTR_8g073570 41.270 126 70 2 1 126 49 170 3.30e-19 84.0
MsG0480018234.01.T01 MTR_5g096050 40.000 120 48 2 183 290 1 108 6.30e-19 82.0
MsG0480018234.01.T01 MTR_5g037190 30.000 220 116 8 1 206 55 250 1.23e-17 81.6
MsG0480018234.01.T01 MTR_3g006670 28.571 252 138 9 66 310 144 360 8.50e-16 78.2
MsG0480018234.01.T01 MTR_1g097330 31.197 234 121 11 55 283 99 297 1.06e-14 75.1
MsG0480018234.01.T01 MTR_2g026010 24.518 363 206 18 6 342 40 360 2.98e-14 73.6
MsG0480018234.01.T01 MTR_4g117720 26.596 282 151 11 84 344 124 370 3.03e-14 73.6
MsG0480018234.01.T01 MTR_5g091150 27.727 220 107 8 44 258 19 191 4.20e-14 70.9
MsG0480018234.01.T01 MTR_3g047540 47.143 70 33 2 152 218 2 70 8.37e-14 66.6
MsG0480018234.01.T01 MTR_4g094400 36.752 117 47 4 180 281 7 111 2.51e-13 66.6
MsG0480018234.01.T01 MTR_2g024100 26.531 245 138 10 80 305 116 337 2.72e-12 67.8
MsG0480018234.01.T01 MTR_6g060650 26.449 276 155 12 81 342 123 364 2.90e-12 67.4
MsG0480018234.01.T01 MTR_0115s0020 23.662 355 215 14 2 326 46 374 7.33e-12 66.2
MsG0480018234.01.T01 MTR_4g023130 26.316 228 119 9 84 301 77 265 9.92e-12 65.5
MsG0480018234.01.T01 MTR_2g021150 26.217 267 149 14 50 304 100 330 1.04e-11 65.9
MsG0480018234.01.T01 MTR_7g079370 25.556 270 153 11 81 332 93 332 1.08e-11 65.9
MsG0480018234.01.T01 MTR_5g057710 25.333 225 127 10 71 288 145 335 1.67e-11 65.5
MsG0480018234.01.T01 MTR_1g068875 23.739 337 196 16 5 321 39 334 3.03e-11 64.3
MsG0480018234.01.T01 MTR_4g061340 25.000 236 132 11 71 293 122 325 3.12e-11 64.3
MsG0480018234.01.T01 MTR_2g021050 23.295 352 188 16 6 321 25 330 3.64e-11 64.3
MsG0480018234.01.T01 MTR_8g090195 49.123 57 29 0 266 322 57 113 3.75e-11 60.1
MsG0480018234.01.T01 MTR_3g097190 50.000 54 27 0 268 321 28 81 4.40e-11 61.6
MsG0480018234.01.T01 MTR_4g023080 26.316 228 119 9 84 301 118 306 5.72e-11 63.5
MsG0480018234.01.T01 MTR_1g068835 25.000 276 152 16 56 309 80 322 7.14e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 73 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGATTTGGTGGTGTTAAA+TGG 0.207464 4:+2014214 MsG0480018234.01.T01:CDS
ATGCTATAAATCTCCCAAAA+AGG 0.231462 4:-2013950 None:intergenic
CACCCAGATCTTCAATTGTT+TGG 0.246054 4:-2014441 None:intergenic
ATGATGAATTTGTTGCATTA+TGG 0.252855 4:+2013729 MsG0480018234.01.T01:CDS
GTTTCGCCGAATTAGGAATT+AGG 0.253803 4:+2014519 MsG0480018234.01.T01:CDS
TATACTAGTATGTTGGATTC+TGG 0.258550 4:+2013674 MsG0480018234.01.T01:CDS
ACCAGAGAGCAAATATAAAT+TGG 0.262515 4:-2013591 None:intergenic
TGGTTTCATCTTCATGGATA+TGG 0.263987 4:+2013821 MsG0480018234.01.T01:CDS
ATTGAAGATCTGGGTGTTAA+AGG 0.267472 4:+2014448 MsG0480018234.01.T01:CDS
ACGGTTTGGTTTCATCTTCA+TGG 0.286237 4:+2013815 MsG0480018234.01.T01:CDS
ATCTTATAGTCGTCATTAAC+AGG 0.293168 4:-2013851 None:intergenic
TGAAACCAAACCGTATTAAA+AGG 0.313213 4:-2013806 None:intergenic
TTAGATATGCCTATAGTAAT+AGG 0.323097 4:+2014001 MsG0480018234.01.T01:CDS
ATAGCATAAGAAGTCATTCT+TGG 0.329702 4:+2013966 MsG0480018234.01.T01:CDS
ACCCAGATCTTCAATTGTTT+GGG 0.334960 4:-2014440 None:intergenic
TACCCAAACAATTGAAGATC+TGG 0.340404 4:+2014438 MsG0480018234.01.T01:CDS
CCTAGCGTGGAGCGTCCTAT+TGG 0.343627 4:+2014349 MsG0480018234.01.T01:CDS
TCCAATTTATATTTGCTCTC+TGG 0.359683 4:+2013590 MsG0480018234.01.T01:CDS
TGGTGAGAAATTTGAGAATA+AGG 0.366936 4:+2013610 MsG0480018234.01.T01:CDS
AAAATACCTAATTCCTAATT+CGG 0.368563 4:-2014525 None:intergenic
GTCCAATCAACATTGTCATT+AGG 0.371785 4:-2013905 None:intergenic
ACGATGCATGGATATGAAAT+TGG 0.373361 4:-2013501 None:intergenic
AGAATAAGGTCTATTTGAAA+TGG 0.374892 4:+2013624 MsG0480018234.01.T01:CDS
AATTCCAATACCTTAGTAGT+TGG 0.394196 4:-2013755 None:intergenic
TTGATTTGGTGGTGTTAAAT+GGG 0.395370 4:+2014215 MsG0480018234.01.T01:CDS
GAATCCAACTACTAAGGTAT+TGG 0.400828 4:+2013751 MsG0480018234.01.T01:CDS
TCATCCTGTTGAAACGAAAC+TGG 0.401243 4:-2013647 None:intergenic
TTGGGTATTTAAATCAAAAC+AGG 0.402196 4:-2014422 None:intergenic
ATGGCCAGTTTCGTTTCAAC+AGG 0.404053 4:+2013643 MsG0480018234.01.T01:CDS
AACCTAATGACAATGTTGAT+TGG 0.412338 4:+2013903 MsG0480018234.01.T01:CDS
TGGACTAAACTCTTCATTGT+TGG 0.431221 4:+2014319 MsG0480018234.01.T01:CDS
TGAGATTTATACTAGTATGT+TGG 0.444568 4:+2013667 MsG0480018234.01.T01:CDS
GGTGAAGAGATACTTTGATT+TGG 0.449012 4:+2014201 MsG0480018234.01.T01:CDS
CGTTACTACACATTTACCCT+TGG 0.452434 4:+2014120 MsG0480018234.01.T01:CDS
GACAAAGTGATCACGAGGAG+TGG 0.456769 4:+2014179 MsG0480018234.01.T01:CDS
GGTCGTAAGGTGTACTTGAA+TGG 0.473243 4:+2014022 MsG0480018234.01.T01:CDS
TTGTCTCTCCACCCACTCAT+CGG 0.477861 4:-2014161 None:intergenic
AATGACGACTATAAGATCAT+TGG 0.493034 4:+2013857 MsG0480018234.01.T01:CDS
GAAGAGATACTTTGATTTGG+TGG 0.495675 4:+2014204 MsG0480018234.01.T01:CDS
TGTCTCTCCACCCACTCATC+GGG 0.497681 4:-2014160 None:intergenic
ACCTTACGACCTATTACTAT+AGG 0.507718 4:-2014010 None:intergenic
CCAATAGGACGCTCCACGCT+AGG 0.509674 4:-2014349 None:intergenic
GCCTATAGTAATAGGTCGTA+AGG 0.510291 4:+2014009 MsG0480018234.01.T01:CDS
GGAATTTGTTCCTTGTAGCC+CGG 0.522715 4:+2013772 MsG0480018234.01.T01:CDS
ATAGCTTTCTTGCATATCCA+AGG 0.527711 4:-2014137 None:intergenic
TATAAATTGGAACCCTCTTG+TGG 0.529967 4:-2013578 None:intergenic
ATTATGGAATCCAACTACTA+AGG 0.536354 4:+2013745 MsG0480018234.01.T01:CDS
AGATGGAGCTGATGCATACA+TGG 0.540461 4:+2014069 MsG0480018234.01.T01:CDS
GTGGAGCGTCCTATTGGAGC+AGG 0.543004 4:+2014355 MsG0480018234.01.T01:CDS
ACTTGAATGGAGTGTGCCAT+TGG 0.544988 4:+2014035 MsG0480018234.01.T01:CDS
ACCCAAACAATTGAAGATCT+GGG 0.545614 4:+2014439 MsG0480018234.01.T01:CDS
CATCTGTATCCGCCCACCAA+TGG 0.546303 4:-2014051 None:intergenic
AAGAAAGCTATCCCGATGAG+TGG 0.547028 4:+2014149 MsG0480018234.01.T01:CDS
ATTGTATAACGAACGATGCA+TGG 0.548537 4:-2013513 None:intergenic
TATGATCTCAAAGCATGTAA+AGG 0.550893 4:+2014246 MsG0480018234.01.T01:CDS
TGTTGGACCCTTGCCTAGCG+TGG 0.551040 4:+2014336 MsG0480018234.01.T01:CDS
TAATTCGGCGAAACAAAAGA+AGG 0.551996 4:-2014510 None:intergenic
TTGGAACCCTCTTGTGGATG+TGG 0.552257 4:-2013572 None:intergenic
TGAATGGAGTGTGCCATTGG+TGG 0.556775 4:+2014038 MsG0480018234.01.T01:CDS
AGGACGCTCCACGCTAGGCA+AGG 0.563463 4:-2014344 None:intergenic
ACAAAGTGATCACGAGGAGT+GGG 0.576937 4:+2014180 MsG0480018234.01.T01:CDS
AACTTGGTGTTAGAGAATCA+TGG 0.580239 4:+2014299 MsG0480018234.01.T01:CDS
GGACGCTCCACGCTAGGCAA+GGG 0.596284 4:-2014343 None:intergenic
CATTGGTGGGCGGATACAGA+TGG 0.597951 4:+2014052 MsG0480018234.01.T01:CDS
TAGCTTTCTTGCATATCCAA+GGG 0.609335 4:-2014136 None:intergenic
GAATGGAGTGTGCCATTGGT+GGG 0.611168 4:+2014039 MsG0480018234.01.T01:CDS
AAGCTATCCCGATGAGTGGG+TGG 0.633452 4:+2014153 MsG0480018234.01.T01:CDS
GATATTGCCACATCCACAAG+AGG 0.634472 4:+2013565 MsG0480018234.01.T01:CDS
AGAAAGCTATCCCGATGAGT+GGG 0.637828 4:+2014150 MsG0480018234.01.T01:CDS
TCTTATAGTCGTCATTAACA+GGG 0.656418 4:-2013850 None:intergenic
TGGAGTGTGCCATTGGTGGG+CGG 0.687105 4:+2014042 MsG0480018234.01.T01:CDS
GGAGAGACAAAGTGATCACG+AGG 0.697413 4:+2014174 MsG0480018234.01.T01:CDS
ATATTGCCACATCCACAAGA+GGG 0.703006 4:+2013566 MsG0480018234.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GCAATATCTCTTTTATATTT+AGG - Chr4:2013553-2013572 None:intergenic 20.0%
!!! TCAGTTTTTACCTTTTAATA+CGG + Chr4:2013796-2013815 MsG0480018234.01.T01:CDS 20.0%
!!! TCTTTTCACATATATGTTTT+GGG + Chr4:2014274-2014293 MsG0480018234.01.T01:CDS 20.0%
!!! TTCTTTTCACATATATGTTT+TGG + Chr4:2014273-2014292 MsG0480018234.01.T01:CDS 20.0%
! AGAATAAGGTCTATTTGAAA+TGG + Chr4:2013624-2013643 MsG0480018234.01.T01:CDS 25.0%
! TGAGATTTATACTAGTATGT+TGG + Chr4:2013667-2013686 MsG0480018234.01.T01:CDS 25.0%
! TTAGATATGCCTATAGTAAT+AGG + Chr4:2014001-2014020 MsG0480018234.01.T01:CDS 25.0%
! TTGGGTATTTAAATCAAAAC+AGG - Chr4:2014425-2014444 None:intergenic 25.0%
!! TTTTACCTTTTAATACGGTT+TGG + Chr4:2013801-2013820 MsG0480018234.01.T01:CDS 25.0%
!!! ATGATGAATTTGTTGCATTA+TGG + Chr4:2013729-2013748 MsG0480018234.01.T01:CDS 25.0%
AACCTAATGACAATGTTGAT+TGG + Chr4:2013903-2013922 MsG0480018234.01.T01:CDS 30.0%
AATGACGACTATAAGATCAT+TGG + Chr4:2013857-2013876 MsG0480018234.01.T01:CDS 30.0%
AATTCCAATACCTTAGTAGT+TGG - Chr4:2013758-2013777 None:intergenic 30.0%
ACCAGAGAGCAAATATAAAT+TGG - Chr4:2013594-2013613 None:intergenic 30.0%
ATAGCATAAGAAGTCATTCT+TGG + Chr4:2013966-2013985 MsG0480018234.01.T01:CDS 30.0%
ATCTTATAGTCGTCATTAAC+AGG - Chr4:2013854-2013873 None:intergenic 30.0%
ATGCTATAAATCTCCCAAAA+AGG - Chr4:2013953-2013972 None:intergenic 30.0%
ATTATGGAATCCAACTACTA+AGG + Chr4:2013745-2013764 MsG0480018234.01.T01:CDS 30.0%
TATACTAGTATGTTGGATTC+TGG + Chr4:2013674-2013693 MsG0480018234.01.T01:CDS 30.0%
TATGATCTCAAAGCATGTAA+AGG + Chr4:2014246-2014265 MsG0480018234.01.T01:CDS 30.0%
TCCAATTTATATTTGCTCTC+TGG + Chr4:2013590-2013609 MsG0480018234.01.T01:CDS 30.0%
TCTTATAGTCGTCATTAACA+GGG - Chr4:2013853-2013872 None:intergenic 30.0%
TGAAACCAAACCGTATTAAA+AGG - Chr4:2013809-2013828 None:intergenic 30.0%
TGCTATAAATCTCCCAAAAA+GGG - Chr4:2013952-2013971 None:intergenic 30.0%
TGGTGAGAAATTTGAGAATA+AGG + Chr4:2013610-2013629 MsG0480018234.01.T01:CDS 30.0%
TTAAAAGGTAAAAACTGAGC+CGG - Chr4:2013794-2013813 None:intergenic 30.0%
!! TTGATTTGGTGGTGTTAAAT+GGG + Chr4:2014215-2014234 MsG0480018234.01.T01:CDS 30.0%
!! TTTGATTTGGTGGTGTTAAA+TGG + Chr4:2014214-2014233 MsG0480018234.01.T01:CDS 30.0%
ACCCAAACAATTGAAGATCT+GGG + Chr4:2014439-2014458 MsG0480018234.01.T01:CDS 35.0%
ACCCAGATCTTCAATTGTTT+GGG - Chr4:2014443-2014462 None:intergenic 35.0%
ACCTTACGACCTATTACTAT+AGG - Chr4:2014013-2014032 None:intergenic 35.0%
ACGATGCATGGATATGAAAT+TGG - Chr4:2013504-2013523 None:intergenic 35.0%
ATAGCTTTCTTGCATATCCA+AGG - Chr4:2014140-2014159 None:intergenic 35.0%
ATTGAAGATCTGGGTGTTAA+AGG + Chr4:2014448-2014467 MsG0480018234.01.T01:CDS 35.0%
ATTGTATAACGAACGATGCA+TGG - Chr4:2013516-2013535 None:intergenic 35.0%
GAATCCAACTACTAAGGTAT+TGG + Chr4:2013751-2013770 MsG0480018234.01.T01:CDS 35.0%
GGTGAAGAGATACTTTGATT+TGG + Chr4:2014201-2014220 MsG0480018234.01.T01:CDS 35.0%
GTCCAATCAACATTGTCATT+AGG - Chr4:2013908-2013927 None:intergenic 35.0%
TAAAAGGTAAAAACTGAGCC+GGG - Chr4:2013793-2013812 None:intergenic 35.0%
TAATTCGGCGAAACAAAAGA+AGG - Chr4:2014513-2014532 None:intergenic 35.0%
TACCCAAACAATTGAAGATC+TGG + Chr4:2014438-2014457 MsG0480018234.01.T01:CDS 35.0%
TAGCTTTCTTGCATATCCAA+GGG - Chr4:2014139-2014158 None:intergenic 35.0%
TATAAATTGGAACCCTCTTG+TGG - Chr4:2013581-2013600 None:intergenic 35.0%
TGGACTAAACTCTTCATTGT+TGG + Chr4:2014319-2014338 MsG0480018234.01.T01:CDS 35.0%
TGGTTTCATCTTCATGGATA+TGG + Chr4:2013821-2013840 MsG0480018234.01.T01:CDS 35.0%
!! AACTTGGTGTTAGAGAATCA+TGG + Chr4:2014299-2014318 MsG0480018234.01.T01:CDS 35.0%
!! ATATATGTTTTGGGCGAACT+TGG + Chr4:2014283-2014302 MsG0480018234.01.T01:CDS 35.0%
!! GAAGAGATACTTTGATTTGG+TGG + Chr4:2014204-2014223 MsG0480018234.01.T01:CDS 35.0%
!! TTCTTTTGTTTCGCCGAATT+AGG + Chr4:2014512-2014531 MsG0480018234.01.T01:CDS 35.0%
ATATTGCCACATCCACAAGA+GGG + Chr4:2013566-2013585 MsG0480018234.01.T01:CDS 40.0%
CACCCAGATCTTCAATTGTT+TGG - Chr4:2014444-2014463 None:intergenic 40.0%
CCTATTGGAGCAGGAAAAAA+AGG + Chr4:2014364-2014383 MsG0480018234.01.T01:CDS 40.0%
CGTTACTACACATTTACCCT+TGG + Chr4:2014120-2014139 MsG0480018234.01.T01:CDS 40.0%
GCCTATAGTAATAGGTCGTA+AGG + Chr4:2014009-2014028 MsG0480018234.01.T01:CDS 40.0%
TAAATCTCCCAAAAAGGGTG+TGG - Chr4:2013947-2013966 None:intergenic 40.0%
TCATCCTGTTGAAACGAAAC+TGG - Chr4:2013650-2013669 None:intergenic 40.0%
!! ACGGTTTGGTTTCATCTTCA+TGG + Chr4:2013815-2013834 MsG0480018234.01.T01:CDS 40.0%
!!! CCTTTTTTTCCTGCTCCAAT+AGG - Chr4:2014367-2014386 None:intergenic 40.0%
AAGAAAGCTATCCCGATGAG+TGG + Chr4:2014149-2014168 MsG0480018234.01.T01:CDS 45.0%
ACAAAGTGATCACGAGGAGT+GGG + Chr4:2014180-2014199 MsG0480018234.01.T01:CDS 45.0%
ACTTGAATGGAGTGTGCCAT+TGG + Chr4:2014035-2014054 MsG0480018234.01.T01:CDS 45.0%
AGAAAGCTATCCCGATGAGT+GGG + Chr4:2014150-2014169 MsG0480018234.01.T01:CDS 45.0%
AGATGGAGCTGATGCATACA+TGG + Chr4:2014069-2014088 MsG0480018234.01.T01:CDS 45.0%
ATGGCCAGTTTCGTTTCAAC+AGG + Chr4:2013643-2013662 MsG0480018234.01.T01:CDS 45.0%
GATATTGCCACATCCACAAG+AGG + Chr4:2013565-2013584 MsG0480018234.01.T01:CDS 45.0%
GGTCGTAAGGTGTACTTGAA+TGG + Chr4:2014022-2014041 MsG0480018234.01.T01:CDS 45.0%
! GGAATTTGTTCCTTGTAGCC+CGG + Chr4:2013772-2013791 MsG0480018234.01.T01:CDS 45.0%
AAAAACTGAGCCGGGCTACA+AGG - Chr4:2013785-2013804 None:intergenic 50.0%
GACAAAGTGATCACGAGGAG+TGG + Chr4:2014179-2014198 MsG0480018234.01.T01:CDS 50.0%
GGAGAGACAAAGTGATCACG+AGG + Chr4:2014174-2014193 MsG0480018234.01.T01:CDS 50.0%
TTGGAACCCTCTTGTGGATG+TGG - Chr4:2013575-2013594 None:intergenic 50.0%
TTGTCTCTCCACCCACTCAT+CGG - Chr4:2014164-2014183 None:intergenic 50.0%
! AACGACGCCACACCCTTTTT+GGG + Chr4:2013937-2013956 MsG0480018234.01.T01:CDS 50.0%
! GAATGGAGTGTGCCATTGGT+GGG + Chr4:2014039-2014058 MsG0480018234.01.T01:CDS 50.0%
! TGAATGGAGTGTGCCATTGG+TGG + Chr4:2014038-2014057 MsG0480018234.01.T01:CDS 50.0%
AAGCTATCCCGATGAGTGGG+TGG + Chr4:2014153-2014172 MsG0480018234.01.T01:CDS 55.0%
CATCTGTATCCGCCCACCAA+TGG - Chr4:2014054-2014073 None:intergenic 55.0%
TGTCTCTCCACCCACTCATC+GGG - Chr4:2014163-2014182 None:intergenic 55.0%
! CAAAAAGGGTGTGGCGTCGT+TGG - Chr4:2013938-2013957 None:intergenic 55.0%
! CAACGACGCCACACCCTTTT+TGG + Chr4:2013936-2013955 MsG0480018234.01.T01:CDS 55.0%
! CATTGGTGGGCGGATACAGA+TGG + Chr4:2014052-2014071 MsG0480018234.01.T01:CDS 55.0%
CCAATAGGACGCTCCACGCT+AGG - Chr4:2014352-2014371 None:intergenic 60.0%
TGTTGGACCCTTGCCTAGCG+TGG + Chr4:2014336-2014355 MsG0480018234.01.T01:CDS 60.0%
! CCTAGCGTGGAGCGTCCTAT+TGG + Chr4:2014349-2014368 MsG0480018234.01.T01:CDS 60.0%
! GTGGAGCGTCCTATTGGAGC+AGG + Chr4:2014355-2014374 MsG0480018234.01.T01:CDS 60.0%
! TGGAGTGTGCCATTGGTGGG+CGG + Chr4:2014042-2014061 MsG0480018234.01.T01:CDS 60.0%
AGGACGCTCCACGCTAGGCA+AGG - Chr4:2014347-2014366 None:intergenic 65.0%
GGACGCTCCACGCTAGGCAA+GGG - Chr4:2014346-2014365 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 2013491 2014540 2013491 ID=MsG0480018234.01;Name=MsG0480018234.01
Chr4 mRNA 2013491 2014540 2013491 ID=MsG0480018234.01.T01;Parent=MsG0480018234.01;Name=MsG0480018234.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|349
Chr4 exon 2013491 2014540 2013491 ID=MsG0480018234.01.T01:exon:643;Parent=MsG0480018234.01.T01
Chr4 CDS 2013491 2014540 2013491 ID=MsG0480018234.01.T01:cds;Parent=MsG0480018234.01.T01
Gene Sequence

>MsG0480018234.01.T01

ATGTTTCGCACCAATTTCATATCCATGCATCGTTCGTTATACAATGACACACATCTCTTCCTAAATATAAAAGAGATATTGCCACATCCACAAGAGGGTTCCAATTTATATTTGCTCTCTGGTGAGAAATTTGAGAATAAGGTCTATTTGAAATGGCCAGTTTCGTTTCAACAGGATGAGATTTATACTAGTATGTTGGATTCTGGTATTAACGATGTTCTTTGTTTCTCTGATCTTGATGATGAATTTGTTGCATTATGGAATCCAACTACTAAGGTATTGGAATTTGTTCCTTGTAGCCCGGCTCAGTTTTTACCTTTTAATACGGTTTGGTTTCATCTTCATGGATATGGTTATGACCCTGTTAATGACGACTATAAGATCATTGGATGTGTTGATGTTAATCAATATAAACCTAATGACAATGTTGATTGGACTTATCTGCCAACGACGCCACACCCTTTTTGGGAGATTTATAGCATAAGAAGTCATTCTTGGAAGAGACTTGATTTAGATATGCCTATAGTAATAGGTCGTAAGGTGTACTTGAATGGAGTGTGCCATTGGTGGGCGGATACAGATGGAGCTGATGCATACATGGTTTCTTTTAACTTGAGCAATGAAAACTTCGTTACTACACATTTACCCTTGGATATGCAAGAAAGCTATCCCGATGAGTGGGTGGAGAGACAAAGTGATCACGAGGAGTGGGTGAAGAGATACTTTGATTTGGTGGTGTTAAATGGGTTTGTTGCTATGATCTCAAAGCATGTAAAGGCATCTTCTTTTCACATATATGTTTTGGGCGAACTTGGTGTTAGAGAATCATGGACTAAACTCTTCATTGTTGGACCCTTGCCTAGCGTGGAGCGTCCTATTGGAGCAGGAAAAAAAGGTGATATATTCTTTCTAAAAAATGACGATGAAGTAGCCTGTTTTGATTTAAATACCCAAACAATTGAAGATCTGGGTGTTAAAGGAGAGTCTTTTTGTTGTCACTTTGCAATTTGTAAAAAAAGCCTTCTTTTGTTTCGCCGAATTAGGAATTAG
Protein sequence

>MsG0480018234.01.T01

MFRTNFISMHRSLYNDTHLFLNIKEILPHPQEGSNLYLLSGEKFENKVYLKWPVSFQQDEIYTSMLDSGINDVLCFSDLDDEFVALWNPTTKVLEFVPCSPAQFLPFNTVWFHLHGYGYDPVNDDYKIIGCVDVNQYKPNDNVDWTYLPTTPHPFWEIYSIRSHSWKRLDLDMPIVIGRKVYLNGVCHWWADTDGADAYMVSFNLSNENFVTTHLPLDMQESYPDEWVERQSDHEEWVKRYFDLVVLNGFVAMISKHVKASSFHIYVLGELGVRESWTKLFIVGPLPSVERPIGAGKKGDIFFLKNDDEVACFDLNTQTIEDLGVKGESFCCHFAICKKSLLLFRRIRN*