AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680035825.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035825.01.T01 MTR_6g086825 84.127 252 40 0 20 271 210 461 9.88e-160 452
MsG0680035825.01.T01 MTR_6g079790 77.165 254 56 1 20 271 57 310 1.29e-146 413
MsG0680035825.01.T01 MTR_6g478230 71.648 261 47 2 20 271 166 408 7.25e-131 377
MsG0680035825.01.T01 MTR_0134s0060 60.674 267 86 4 18 271 153 413 1.10e-101 303
MsG0680035825.01.T01 MTR_6g087620 62.857 245 58 4 18 260 66 279 1.03e-100 295
MsG0680035825.01.T01 MTR_6g078010 60.324 247 74 4 19 265 108 330 5.29e-98 291
MsG0680035825.01.T01 MTR_6g079770 55.556 279 107 4 5 271 145 418 2.96e-97 291
MsG0680035825.01.T01 MTR_0054s0100 60.744 242 81 3 35 271 177 409 4.62e-97 291
MsG0680035825.01.T01 MTR_0054s0090 59.917 242 83 3 35 271 175 407 2.81e-95 286
MsG0680035825.01.T01 MTR_6g079810 57.088 261 95 4 18 268 208 461 7.87e-94 284
MsG0680035825.01.T01 MTR_6g080680 57.895 247 84 3 19 264 162 389 2.26e-92 278
MsG0680035825.01.T01 MTR_6g090260 65.700 207 62 4 64 267 61 261 1.16e-90 269
MsG0680035825.01.T01 MTR_6g090180 58.431 255 92 4 19 267 189 435 1.77e-90 275
MsG0680035825.01.T01 MTR_4g130330 54.737 285 101 8 2 270 119 391 1.05e-89 271
MsG0680035825.01.T01 MTR_4g028510 56.471 255 98 5 19 270 158 402 2.28e-88 270
MsG0680035825.01.T01 MTR_6g090250 57.787 244 88 4 37 267 180 421 8.82e-88 267
MsG0680035825.01.T01 MTR_4g028660 52.727 275 111 6 2 267 132 396 5.40e-84 257
MsG0680035825.01.T01 MTR_4g027450 52.727 275 111 6 2 267 132 396 5.40e-84 257
MsG0680035825.01.T01 MTR_4g028680 50.890 281 115 7 2 271 133 401 1.07e-82 254
MsG0680035825.01.T01 MTR_8g063440 51.250 240 96 5 37 271 161 384 1.41e-70 222
MsG0680035825.01.T01 MTR_5g076490 54.132 242 96 8 37 270 102 336 5.65e-70 219
MsG0680035825.01.T01 MTR_4g028750 51.515 231 89 7 25 248 162 376 7.60e-70 221
MsG0680035825.01.T01 MTR_6g461660 47.970 271 124 5 3 270 144 400 2.04e-68 217
MsG0680035825.01.T01 MTR_5g097330 50.424 236 105 5 37 270 178 403 3.62e-68 216
MsG0680035825.01.T01 MTR_0129s0070 49.388 245 102 4 36 271 166 397 1.27e-67 215
MsG0680035825.01.T01 MTR_5g097680 50.198 253 108 8 26 271 187 428 2.42e-67 215
MsG0680035825.01.T01 MTR_5g097220 50.199 251 96 6 37 270 170 408 2.71e-67 214
MsG0680035825.01.T01 MTR_7g023800 46.825 252 86 3 18 267 103 308 1.11e-66 210
MsG0680035825.01.T01 MTR_0129s0120 48.971 243 102 4 36 269 166 395 1.37e-66 212
MsG0680035825.01.T01 MTR_2g021800 47.410 251 113 4 33 271 174 417 2.48e-66 212
MsG0680035825.01.T01 MTR_6g079730 69.444 144 44 0 124 267 4 147 4.58e-66 202
MsG0680035825.01.T01 MTR_6g004450 46.415 265 118 8 13 269 20 268 5.53e-66 207
MsG0680035825.01.T01 MTR_2g022000 47.581 248 109 5 33 267 173 412 2.11e-65 210
MsG0680035825.01.T01 MTR_5g064700 49.593 246 113 7 30 270 129 368 4.81e-65 207
MsG0680035825.01.T01 MTR_2g021920 44.118 272 129 6 8 266 8 269 9.31e-65 204
MsG0680035825.01.T01 MTR_5g037680 49.569 232 90 7 38 264 171 380 1.87e-62 203
MsG0680035825.01.T01 MTR_2g021950 49.785 233 103 5 38 266 171 393 5.08e-62 200
MsG0680035825.01.T01 MTR_4g102410 48.333 240 110 6 36 268 216 448 1.07e-61 201
MsG0680035825.01.T01 MTR_0129s0050 45.276 254 110 4 37 270 164 408 1.83e-61 199
MsG0680035825.01.T01 MTR_4g117550 44.151 265 109 5 11 271 145 374 5.96e-61 197
MsG0680035825.01.T01 MTR_5g096630 44.615 260 123 7 25 270 162 414 1.01e-60 197
MsG0680035825.01.T01 MTR_2g038680 45.635 252 109 8 34 269 171 410 1.59e-60 197
MsG0680035825.01.T01 MTR_5g069390 43.621 243 123 5 31 270 186 417 2.05e-60 199
MsG0680035825.01.T01 MTR_2g021770 45.161 248 110 6 39 271 172 408 3.94e-60 196
MsG0680035825.01.T01 MTR_2g104340 45.817 251 109 6 37 271 158 397 4.99e-59 193
MsG0680035825.01.T01 MTR_5g092850 45.749 247 118 6 30 267 160 399 4.82e-58 191
MsG0680035825.01.T01 MTR_3g018750 45.020 251 110 10 37 270 2 241 6.81e-58 185
MsG0680035825.01.T01 MTR_5g092860 46.185 249 102 8 17 247 116 350 3.08e-57 187
MsG0680035825.01.T01 MTR_5g097350 47.210 233 100 8 36 267 174 384 3.28e-57 188
MsG0680035825.01.T01 MTR_7g017130 44.444 234 122 4 38 271 173 398 5.99e-57 187
MsG0680035825.01.T01 MTR_5g023970 50.000 198 89 3 75 271 9 197 9.85e-57 181
MsG0680035825.01.T01 MTR_4g007940 42.857 252 112 8 36 271 107 342 2.64e-56 184
MsG0680035825.01.T01 MTR_2g065860 43.307 254 124 5 20 271 154 389 1.69e-55 183
MsG0680035825.01.T01 MTR_5g059430 41.860 258 120 6 24 268 184 424 2.86e-55 184
MsG0680035825.01.T01 MTR_8g041270 44.223 251 113 6 37 271 168 407 3.93e-55 183
MsG0680035825.01.T01 MTR_8g064890 45.000 240 107 5 37 271 170 389 4.71e-55 182
MsG0680035825.01.T01 MTR_2g066200 43.333 240 116 6 37 271 165 389 1.95e-54 181
MsG0680035825.01.T01 MTR_5g097850 50.510 196 75 5 82 269 63 244 2.37e-54 176
MsG0680035825.01.T01 MTR_3g011120 44.000 225 106 6 37 260 165 370 2.44e-54 180
MsG0680035825.01.T01 MTR_1g007450 46.781 233 106 7 33 256 159 382 2.54e-54 181
MsG0680035825.01.T01 MTR_7g017170 41.870 246 123 5 38 271 173 410 2.90e-54 181
MsG0680035825.01.T01 MTR_8g467670 41.296 247 122 6 26 271 156 380 5.45e-54 179
MsG0680035825.01.T01 MTR_7g111700 45.339 236 93 5 37 271 166 366 2.90e-53 177
MsG0680035825.01.T01 MTR_5g074800 44.167 240 105 7 37 262 112 336 1.24e-52 174
MsG0680035825.01.T01 MTR_5g096910 45.299 234 105 7 38 269 180 392 4.52e-52 174
MsG0680035825.01.T01 MTR_2g065840 42.857 245 114 7 29 269 166 388 5.38e-52 174
MsG0680035825.01.T01 MTR_0831s0010 42.500 240 116 6 37 269 196 420 7.21e-52 175
MsG0680035825.01.T01 MTR_5g043605 41.880 234 115 5 38 270 126 339 7.43e-52 173
MsG0680035825.01.T01 MTR_4g014370 42.292 253 121 6 23 270 177 409 8.38e-52 174
MsG0680035825.01.T01 MTR_2g104280 42.000 250 118 6 37 270 167 405 1.07e-51 174
MsG0680035825.01.T01 MTR_8g464770 42.918 233 116 5 34 266 161 376 1.19e-51 173
MsG0680035825.01.T01 MTR_5g068490 41.803 244 115 7 28 271 62 278 1.28e-51 170
MsG0680035825.01.T01 MTR_2g066170 41.245 257 128 7 23 271 146 387 1.47e-51 173
MsG0680035825.01.T01 MTR_2g034700 42.231 251 111 6 23 267 151 373 4.87e-51 171
MsG0680035825.01.T01 MTR_8g012090 46.078 204 70 3 37 239 170 334 1.68e-50 169
MsG0680035825.01.T01 MTR_4g055820 45.455 209 85 5 35 226 163 359 2.02e-50 169
MsG0680035825.01.T01 MTR_5g074850 45.339 236 102 9 31 251 182 405 2.79e-50 177
MsG0680035825.01.T01 MTR_5g086590 45.050 202 95 3 41 242 46 231 9.78e-50 164
MsG0680035825.01.T01 MTR_7g069550 43.952 248 106 10 37 271 169 396 1.27e-49 168
MsG0680035825.01.T01 MTR_6g009350 46.231 199 97 5 36 233 6 195 2.72e-49 161
MsG0680035825.01.T01 MTR_2g064950 44.215 242 104 10 38 261 153 381 9.03e-49 166
MsG0680035825.01.T01 MTR_5g075370 45.923 233 97 10 31 247 158 377 2.21e-48 171
MsG0680035825.01.T01 MTR_5g091140 45.333 225 103 8 37 249 180 396 3.06e-48 165
MsG0680035825.01.T01 MTR_8g033140 39.015 264 111 9 39 271 175 419 1.01e-47 164
MsG0680035825.01.T01 MTR_7g079640 42.520 254 106 10 20 267 153 372 5.32e-46 158
MsG0680035825.01.T01 MTR_5g022850 41.270 252 121 7 33 271 117 354 6.45e-46 157
MsG0680035825.01.T01 MTR_1g007410 43.621 243 109 10 38 267 176 403 2.68e-45 157
MsG0680035825.01.T01 MTR_4g014370 43.781 201 95 3 74 270 151 337 1.21e-44 154
MsG0680035825.01.T01 MTR_7g017040 42.017 238 126 6 38 270 168 398 3.87e-44 154
MsG0680035825.01.T01 MTR_2g066150 41.079 241 101 7 37 271 156 361 5.31e-44 153
MsG0680035825.01.T01 MTR_5g066420 47.712 153 75 2 108 259 1 149 1.44e-43 145
MsG0680035825.01.T01 MTR_8g046310 40.086 232 113 5 38 266 166 374 2.66e-43 151
MsG0680035825.01.T01 MTR_7g017150 46.429 196 95 5 77 271 82 268 2.39e-42 146
MsG0680035825.01.T01 MTR_7g104710 54.745 137 53 1 68 204 169 296 2.69e-42 147
MsG0680035825.01.T01 MTR_8g018110 37.354 257 112 9 38 270 166 397 3.35e-42 149
MsG0680035825.01.T01 MTR_8g032980 36.268 284 124 8 29 271 155 422 8.10e-42 148
MsG0680035825.01.T01 MTR_8g046200 35.974 303 136 10 1 266 137 418 8.54e-42 148
MsG0680035825.01.T01 MTR_8g033090 38.168 262 115 8 39 270 168 412 8.55e-42 148
MsG0680035825.01.T01 MTR_8g035690 36.177 293 135 10 8 269 31 302 1.16e-41 145
MsG0680035825.01.T01 MTR_7g017120 45.918 196 96 5 77 271 81 267 1.24e-41 144
MsG0680035825.01.T01 MTR_4g016000 41.038 212 85 6 23 223 11 193 1.91e-41 142
MsG0680035825.01.T01 MTR_1g098180 38.306 248 109 7 37 271 151 367 2.95e-41 145
MsG0680035825.01.T01 MTR_8g036970 46.961 181 77 5 70 248 139 302 3.10e-41 145
MsG0680035825.01.T01 MTR_5g024260 40.851 235 109 9 28 249 168 385 5.51e-41 146
MsG0680035825.01.T01 MTR_8g033000 43.842 203 90 6 77 270 176 363 6.44e-41 145
MsG0680035825.01.T01 MTR_1g007430 42.584 209 92 4 27 228 137 324 7.96e-41 144
MsG0680035825.01.T01 MTR_7g019250 36.434 258 107 8 7 246 194 412 1.49e-40 146
MsG0680035825.01.T01 MTR_8g046220 35.484 279 126 9 23 266 143 402 5.48e-40 143
MsG0680035825.01.T01 MTR_3g030620 44.633 177 85 4 97 268 12 180 5.76e-40 137
MsG0680035825.01.T01 MTR_1g053510 36.290 248 123 3 38 265 169 401 1.36e-39 142
MsG0680035825.01.T01 MTR_5g096920 46.914 162 73 4 108 268 1 150 2.06e-39 135
MsG0680035825.01.T01 MTR_8g063350 37.600 250 122 8 22 261 112 337 2.61e-39 140
MsG0680035825.01.T01 MTR_8g013000 45.933 209 86 8 32 228 160 353 3.50e-39 141
MsG0680035825.01.T01 MTR_8g033110 34.932 292 139 9 11 270 143 415 3.03e-38 139
MsG0680035825.01.T01 MTR_8g039500 37.946 224 115 6 23 239 140 346 3.04e-38 137
MsG0680035825.01.T01 MTR_5g097660 55.652 115 50 1 154 267 3 117 4.96e-38 130
MsG0680035825.01.T01 MTR_5g072060 42.152 223 84 7 37 245 156 347 5.12e-38 141
MsG0680035825.01.T01 MTR_1g050312 39.655 232 100 6 37 262 90 287 6.33e-38 135
MsG0680035825.01.T01 MTR_5g098950 39.367 221 91 5 10 228 95 274 3.88e-37 132
MsG0680035825.01.T01 MTR_8g063370 36.087 230 125 7 37 262 173 384 4.05e-37 135
MsG0680035825.01.T01 MTR_4g094400 57.143 112 32 2 104 204 6 112 3.12e-36 126
MsG0680035825.01.T01 MTR_8g063330 36.986 219 113 5 16 225 146 348 2.19e-35 132
MsG0680035825.01.T01 MTR_8g064790 41.566 166 83 2 106 271 187 338 2.62e-34 127
MsG0680035825.01.T01 MTR_8g063310 35.124 242 124 10 37 262 165 389 6.09e-33 124
MsG0680035825.01.T01 MTR_5g097540 44.444 153 68 5 77 228 97 233 5.59e-32 119
MsG0680035825.01.T01 MTR_5g086780 50.000 116 58 0 131 246 113 228 3.55e-31 115
MsG0680035825.01.T01 MTR_2g022020 47.458 118 60 1 149 266 1 116 8.81e-31 111
MsG0680035825.01.T01 MTR_5g036250 56.701 97 41 1 166 262 8 103 3.65e-30 112
MsG0680035825.01.T01 MTR_7g016930 40.341 176 88 4 30 199 138 302 5.12e-28 110
MsG0680035825.01.T01 MTR_5g094350 45.385 130 60 3 30 155 154 276 2.07e-24 99.4
MsG0680035825.01.T01 MTR_1g050308 50.000 110 50 3 164 271 242 348 6.40e-24 99.4
MsG0680035825.01.T01 MTR_5g096900 39.655 174 82 5 23 193 160 313 1.65e-23 97.8
MsG0680035825.01.T01 MTR_2g026010 30.303 231 122 9 33 245 134 343 3.51e-22 95.1
MsG0680035825.01.T01 MTR_7g101200 37.572 173 79 7 65 227 75 228 2.26e-21 90.1
MsG0680035825.01.T01 MTR_5g096050 44.954 109 55 2 108 212 1 108 3.81e-20 84.0
MsG0680035825.01.T01 MTR_2g426070 31.336 217 115 12 37 243 165 357 3.22e-19 86.7
MsG0680035825.01.T01 MTR_4g133170 27.570 214 128 7 33 240 138 330 5.04e-19 86.3
MsG0680035825.01.T01 MTR_2g028130 28.378 222 126 8 37 243 163 366 1.10e-18 85.5
MsG0680035825.01.T01 MTR_2g028140 29.032 217 126 9 37 243 167 365 3.07e-18 84.0
MsG0680035825.01.T01 MTR_2g426090 28.216 241 137 12 14 243 139 354 4.14e-18 83.6
MsG0680035825.01.T01 MTR_3g083010 26.577 222 131 7 37 243 168 372 5.29e-18 83.6
MsG0680035825.01.T01 MTR_5g091150 45.378 119 44 7 61 176 84 184 1.25e-17 79.3
MsG0680035825.01.T01 MTR_2g025960 25.610 246 142 12 14 242 44 265 4.35e-17 79.7
MsG0680035825.01.T01 MTR_2g024100 28.319 226 127 9 36 243 153 361 6.06e-17 80.1
MsG0680035825.01.T01 MTR_4g070550 27.155 232 142 9 37 258 161 375 9.78e-17 79.7
MsG0680035825.01.T01 MTR_2g425980 27.273 220 124 9 37 242 158 355 1.01e-16 79.7
MsG0680035825.01.T01 MTR_2g028110 30.806 211 122 11 37 243 147 337 1.12e-16 79.3
MsG0680035825.01.T01 MTR_3g097190 61.111 54 21 0 190 243 28 81 1.69e-16 75.9
MsG0680035825.01.T01 MTR_1g115205 46.591 88 43 3 33 116 150 237 3.02e-16 76.6
MsG0680035825.01.T01 MTR_2g104260 38.462 117 55 2 37 144 167 275 3.40e-16 77.0
MsG0680035825.01.T01 MTR_1g050305 48.810 84 38 2 171 249 21 104 3.58e-16 73.9
MsG0680035825.01.T01 MTR_1g068860 28.704 216 126 11 37 243 141 337 4.64e-16 77.4
MsG0680035825.01.T01 MTR_2g031720 27.948 229 137 10 26 243 132 343 3.41e-15 75.1
MsG0680035825.01.T01 MTR_2g031680 28.378 222 127 9 33 242 134 335 1.90e-14 72.8
MsG0680035825.01.T01 MTR_2g426020 26.939 245 135 11 37 265 160 376 5.67e-14 71.6
MsG0680035825.01.T01 MTR_2g021050 28.251 223 120 12 38 243 131 330 1.71e-13 70.1
MsG0680035825.01.T01 MTR_3g047540 43.878 98 36 5 74 169 3 83 7.56e-13 63.5
MsG0680035825.01.T01 MTR_2g025420 25.551 227 129 10 37 243 166 372 4.27e-12 66.2
MsG0680035825.01.T01 MTR_6g070880 44.681 94 35 5 19 99 172 261 7.77e-12 64.3
MsG0680035825.01.T01 MTR_6g071420 44.681 94 35 5 19 99 172 261 7.77e-12 64.3
MsG0680035825.01.T01 MTR_2g024110 24.569 232 133 9 31 242 133 342 8.24e-12 65.1
MsG0680035825.01.T01 MTR_7g017270 63.636 44 16 0 154 197 38 81 1.23e-11 60.1
MsG0680035825.01.T01 MTR_0064s0160 26.016 246 144 11 39 267 196 420 4.65e-11 63.2
MsG0680035825.01.T01 MTR_8g090195 50.000 56 28 0 189 244 58 113 5.60e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035825.01.T01 AT4G12560 24.561 228 140 9 38 248 140 352 4.18e-11 63.2
MsG0680035825.01.T01 AT4G12560 24.561 228 140 9 38 248 140 352 4.18e-11 63.2
MsG0680035825.01.T01 AT4G12560 24.561 228 140 9 38 248 140 352 4.18e-11 63.2

Find 72 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAATATTTCGAACCCTTA+TGG 0.196734 6:-112872232 MsG0680035825.01.T01:CDS
ACCTTTGTGTCTTATCTTCA+TGG 0.196915 6:-112872347 MsG0680035825.01.T01:CDS
AGAAACTTGGCAGTGCTAAA+TGG 0.273887 6:-112871951 MsG0680035825.01.T01:CDS
TAAATTGTTATCCGACATTC+AGG 0.275746 6:+112871687 None:intergenic
AACCAAGCTCCTCAATCATT+TGG 0.279056 6:+112871724 None:intergenic
AGGTTGTATTTCTGAGTAGA+TGG 0.283406 6:+112872426 None:intergenic
GTGTCTTATCTTCATGGATT+TGG 0.287225 6:-112872341 MsG0680035825.01.T01:CDS
TGGTTGTGTCAGAAGGATTA+TGG 0.288305 6:-112872101 MsG0680035825.01.T01:CDS
CAAATATTTCGAACCCTTAT+GGG 0.294700 6:-112872231 MsG0680035825.01.T01:CDS
GTCAGAAGGATTATGGATAT+TGG 0.312134 6:-112872094 MsG0680035825.01.T01:CDS
TATCCCATTTCAACCTAGTT+TGG 0.313670 6:-112872060 MsG0680035825.01.T01:CDS
TGGATCAATCTCTTCACTAT+TGG 0.329723 6:-112871846 MsG0680035825.01.T01:CDS
GATACCAAACTAGGTTGAAA+TGG 0.334259 6:+112872056 None:intergenic
AACCTCCTTCCTCCAAGTTT+CGG 0.364486 6:-112872408 MsG0680035825.01.T01:CDS
CCTTGTGTTGAACGTCCTAT+TGG 0.385776 6:-112871816 MsG0680035825.01.T01:CDS
ATCATCTACATCTGAGGGTA+TGG 0.393256 6:+112871998 None:intergenic
TCATGGATGGAGTGTGTCAT+TGG 0.399727 6:-112872121 MsG0680035825.01.T01:CDS
ACCAAGCTCCTCAATCATTT+GGG 0.408989 6:+112871725 None:intergenic
TAGGCTATATATCTCCCATA+AGG 0.415397 6:+112872217 None:intergenic
GAAACTTGGCAGTGCTAAAT+GGG 0.417001 6:-112871950 MsG0680035825.01.T01:CDS
CAGTCAGCACTGCAGATGCC+TGG 0.420613 6:-112872258 MsG0680035825.01.T01:CDS
TGAGGGAGCTCATGTCTTCA+TGG 0.435101 6:-112872138 MsG0680035825.01.T01:CDS
AAAGAACGCTTAACATCATC+CGG 0.438507 6:+112872371 None:intergenic
TTCGGTTGATTCATATGTCC+CGG 0.446415 6:-112872390 MsG0680035825.01.T01:CDS
GAAAGCATTCTTCCACTTGA+AGG 0.453255 6:-112871660 MsG0680035825.01.T01:CDS
CCCTTCGTACCCACTCCAAT+AGG 0.463699 6:+112871801 None:intergenic
GTTCGAAATATTTGTTTCCC+AGG 0.469085 6:+112872240 None:intergenic
CATATGAATCAACCGAAACT+TGG 0.474594 6:+112872396 None:intergenic
TCCTCCACGAGTTACTTCTT+AGG 0.477396 6:+112872198 None:intergenic
GTGGTTACGAACATGTATTA+TGG 0.486339 6:-112872452 None:intergenic
ATACCAAACTAGGTTGAAAT+GGG 0.489308 6:+112872057 None:intergenic
ACCCAAATGATTGAGGAGCT+TGG 0.502571 6:-112871726 MsG0680035825.01.T01:CDS
CAGTGCTGACTGTTAAGATC+TGG 0.508504 6:+112872269 None:intergenic
TTTGAGTACCCAAATGATTG+AGG 0.510774 6:-112871733 MsG0680035825.01.T01:CDS
TGAAACAAATTGGAGAAACT+TGG 0.511083 6:-112871964 MsG0680035825.01.T01:CDS
GAAAAGATGAAGAACTAGCT+TGG 0.514947 6:-112871761 MsG0680035825.01.T01:CDS
AGCTTGGTGTGAAGGAATCA+TGG 0.516634 6:-112871866 MsG0680035825.01.T01:CDS
CCTATTGGAGTGGGTACGAA+GGG 0.517897 6:-112871801 MsG0680035825.01.T01:CDS
AGTCAGCACTGCAGATGCCT+GGG 0.533183 6:-112872257 MsG0680035825.01.T01:CDS
AGGCTATATATCTCCCATAA+GGG 0.541596 6:+112872218 None:intergenic
TAATTATTTATTCCTTCAAG+TGG 0.548656 6:+112871648 None:intergenic
AAAACAATCATCTACATCTG+AGG 0.552052 6:+112871992 None:intergenic
GTGTCATTGGTTGTGTCAGA+AGG 0.553432 6:-112872108 MsG0680035825.01.T01:CDS
AAGTAAAACGATACCAAACT+AGG 0.554028 6:+112872047 None:intergenic
GTATCAATTATGGGTGAGCT+TGG 0.554666 6:-112871882 MsG0680035825.01.T01:CDS
CCCTCACTATAGTCGAAAGA+AGG 0.561528 6:+112872155 None:intergenic
ATGAATCAACCGAAACTTGG+AGG 0.561998 6:+112872399 None:intergenic
GGAGCTCATGTCTTCATGGA+TGG 0.562816 6:-112872134 MsG0680035825.01.T01:CDS
GGTTGTATTTCTGAGTAGAT+GGG 0.570867 6:+112872427 None:intergenic
ATGTCGGATAACAATTTACA+AGG 0.573289 6:-112871682 MsG0680035825.01.T01:CDS
CCAATAGGACGTTCAACACA+AGG 0.583174 6:+112871816 None:intergenic
AAACAATCATCTACATCTGA+GGG 0.586277 6:+112871993 None:intergenic
TCCTATTGGAGTGGGTACGA+AGG 0.586640 6:-112871802 MsG0680035825.01.T01:CDS
CCTTCTTTCGACTATAGTGA+GGG 0.590875 6:-112872155 MsG0680035825.01.T01:CDS
TCATCTACATCTGAGGGTAT+GGG 0.593933 6:+112871999 None:intergenic
GCCTTCTTTCGACTATAGTG+AGG 0.595670 6:-112872156 MsG0680035825.01.T01:CDS
TATGGGTGAGCTTGGTGTGA+AGG 0.601488 6:-112871874 MsG0680035825.01.T01:CDS
TGTTGAACGTCCTATTGGAG+TGG 0.607214 6:-112871811 MsG0680035825.01.T01:CDS
CTTTACAGTCATTTGCAACA+CGG 0.607217 6:+112872312 None:intergenic
ATCAACCGAAACTTGGAGGA+AGG 0.610927 6:+112872403 None:intergenic
ATAATATGAGATCAATGCAA+TGG 0.614224 6:+112871926 None:intergenic
TATTGGAGTGGGTACGAAGG+GGG 0.628720 6:-112871799 MsG0680035825.01.T01:CDS
AAGTGGATGAACCTGAATGT+CGG 0.631712 6:-112871698 MsG0680035825.01.T01:CDS
GCCTAAGAAGTAACTCGTGG+AGG 0.632578 6:-112872199 MsG0680035825.01.T01:CDS
AACCGAAACTTGGAGGAAGG+AGG 0.640596 6:+112872406 None:intergenic
ATAGCCTAAGAAGTAACTCG+TGG 0.647029 6:-112872202 MsG0680035825.01.T01:CDS
GTTGAACGTCCTATTGGAGT+GGG 0.647750 6:-112871810 MsG0680035825.01.T01:CDS
TCACTATAGTCGAAAGAAGG+CGG 0.647958 6:+112872158 None:intergenic
GGACGTTCAACACAAGGCAA+TGG 0.649002 6:+112871822 None:intergenic
CTATTGGAGTGGGTACGAAG+GGG 0.656356 6:-112871800 MsG0680035825.01.T01:CDS
AAGAACGCTTAACATCATCC+GGG 0.666045 6:+112872372 None:intergenic
TCCATGAAGATAAGACACAA+AGG 0.680230 6:+112872346 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTATTTATTCCTTCAAG+TGG + Chr6:112872442-112872461 None:intergenic 20.0%
! ATAATATGAGATCAATGCAA+TGG + Chr6:112872164-112872183 None:intergenic 25.0%
!! ACTTTTCACGTATCAATTAT+GGG - Chr6:112872196-112872215 MsG0680035825.01.T01:CDS 25.0%
!! TACTTTTCACGTATCAATTA+TGG - Chr6:112872195-112872214 MsG0680035825.01.T01:CDS 25.0%
!!! GTTTTGATGTTGAAACAAAT+TGG - Chr6:112872113-112872132 MsG0680035825.01.T01:CDS 25.0%
AAAACAATCATCTACATCTG+AGG + Chr6:112872098-112872117 None:intergenic 30.0%
AAACAATCATCTACATCTGA+GGG + Chr6:112872097-112872116 None:intergenic 30.0%
AAGTAAAACGATACCAAACT+AGG + Chr6:112872043-112872062 None:intergenic 30.0%
ACAAATATTTCGAACCCTTA+TGG - Chr6:112871855-112871874 MsG0680035825.01.T01:CDS 30.0%
ATGTCGGATAACAATTTACA+AGG - Chr6:112872405-112872424 MsG0680035825.01.T01:CDS 30.0%
CAAATATTTCGAACCCTTAT+GGG - Chr6:112871856-112871875 MsG0680035825.01.T01:CDS 30.0%
TAAATTGTTATCCGACATTC+AGG + Chr6:112872403-112872422 None:intergenic 30.0%
TGAAACAAATTGGAGAAACT+TGG - Chr6:112872123-112872142 MsG0680035825.01.T01:CDS 30.0%
! ATACCAAACTAGGTTGAAAT+GGG + Chr6:112872033-112872052 None:intergenic 30.0%
AAAGAACGCTTAACATCATC+CGG + Chr6:112871719-112871738 None:intergenic 35.0%
ACCTTTGTGTCTTATCTTCA+TGG - Chr6:112871740-112871759 MsG0680035825.01.T01:CDS 35.0%
AGGCTATATATCTCCCATAA+GGG + Chr6:112871872-112871891 None:intergenic 35.0%
CATATGAATCAACCGAAACT+TGG + Chr6:112871694-112871713 None:intergenic 35.0%
CTTTACAGTCATTTGCAACA+CGG + Chr6:112871778-112871797 None:intergenic 35.0%
GAAAAGATGAAGAACTAGCT+TGG - Chr6:112872326-112872345 MsG0680035825.01.T01:CDS 35.0%
GTCAGAAGGATTATGGATAT+TGG - Chr6:112871993-112872012 MsG0680035825.01.T01:CDS 35.0%
GTGTCTTATCTTCATGGATT+TGG - Chr6:112871746-112871765 MsG0680035825.01.T01:CDS 35.0%
GTTCGAAATATTTGTTTCCC+AGG + Chr6:112871850-112871869 None:intergenic 35.0%
TAGGCTATATATCTCCCATA+AGG + Chr6:112871873-112871892 None:intergenic 35.0%
TATCCCATTTCAACCTAGTT+TGG - Chr6:112872027-112872046 MsG0680035825.01.T01:CDS 35.0%
TCCATGAAGATAAGACACAA+AGG + Chr6:112871744-112871763 None:intergenic 35.0%
TGGATCAATCTCTTCACTAT+TGG - Chr6:112872241-112872260 MsG0680035825.01.T01:CDS 35.0%
TTTGAGTACCCAAATGATTG+AGG - Chr6:112872354-112872373 MsG0680035825.01.T01:CDS 35.0%
! AGGTTGTATTTCTGAGTAGA+TGG + Chr6:112871664-112871683 None:intergenic 35.0%
! GATACCAAACTAGGTTGAAA+TGG + Chr6:112872034-112872053 None:intergenic 35.0%
! GGTTGTATTTCTGAGTAGAT+GGG + Chr6:112871663-112871682 None:intergenic 35.0%
AACCAAGCTCCTCAATCATT+TGG + Chr6:112872366-112872385 None:intergenic 40.0%
AAGAACGCTTAACATCATCC+GGG + Chr6:112871718-112871737 None:intergenic 40.0%
AAGTGGATGAACCTGAATGT+CGG - Chr6:112872389-112872408 MsG0680035825.01.T01:CDS 40.0%
ACCAAGCTCCTCAATCATTT+GGG + Chr6:112872365-112872384 None:intergenic 40.0%
AGAAACTTGGCAGTGCTAAA+TGG - Chr6:112872136-112872155 MsG0680035825.01.T01:CDS 40.0%
ATAGCCTAAGAAGTAACTCG+TGG - Chr6:112871885-112871904 MsG0680035825.01.T01:CDS 40.0%
ATCATCTACATCTGAGGGTA+TGG + Chr6:112872092-112872111 None:intergenic 40.0%
ATGAATCAACCGAAACTTGG+AGG + Chr6:112871691-112871710 None:intergenic 40.0%
CCTTCTTTCGACTATAGTGA+GGG - Chr6:112871932-112871951 MsG0680035825.01.T01:CDS 40.0%
GAAACTTGGCAGTGCTAAAT+GGG - Chr6:112872137-112872156 MsG0680035825.01.T01:CDS 40.0%
GTATCAATTATGGGTGAGCT+TGG - Chr6:112872205-112872224 MsG0680035825.01.T01:CDS 40.0%
TCACTATAGTCGAAAGAAGG+CGG + Chr6:112871932-112871951 None:intergenic 40.0%
TGGTTGTGTCAGAAGGATTA+TGG - Chr6:112871986-112872005 MsG0680035825.01.T01:CDS 40.0%
! TCATCTACATCTGAGGGTAT+GGG + Chr6:112872091-112872110 None:intergenic 40.0%
! TTCGGTTGATTCATATGTCC+CGG - Chr6:112871697-112871716 MsG0680035825.01.T01:CDS 40.0%
!! GAAAGCATTCTTCCACTTGA+AGG - Chr6:112872427-112872446 MsG0680035825.01.T01:CDS 40.0%
!!! TGAGGAGCTTGGTTTTAAAG+TGG - Chr6:112872372-112872391 MsG0680035825.01.T01:CDS 40.0%
AACCTCCTTCCTCCAAGTTT+CGG - Chr6:112871679-112871698 MsG0680035825.01.T01:CDS 45.0%
ACCCAAATGATTGAGGAGCT+TGG - Chr6:112872361-112872380 MsG0680035825.01.T01:CDS 45.0%
ATCAACCGAAACTTGGAGGA+AGG + Chr6:112871687-112871706 None:intergenic 45.0%
CCAATAGGACGTTCAACACA+AGG + Chr6:112872274-112872293 None:intergenic 45.0%
CCCTCACTATAGTCGAAAGA+AGG + Chr6:112871935-112871954 None:intergenic 45.0%
CCTTGTGTTGAACGTCCTAT+TGG - Chr6:112872271-112872290 MsG0680035825.01.T01:CDS 45.0%
GCCTTCTTTCGACTATAGTG+AGG - Chr6:112871931-112871950 MsG0680035825.01.T01:CDS 45.0%
GTTGAACGTCCTATTGGAGT+GGG - Chr6:112872277-112872296 MsG0680035825.01.T01:CDS 45.0%
TCATGGATGGAGTGTGTCAT+TGG - Chr6:112871966-112871985 MsG0680035825.01.T01:CDS 45.0%
TCCTCCACGAGTTACTTCTT+AGG + Chr6:112871892-112871911 None:intergenic 45.0%
TGTTGAACGTCCTATTGGAG+TGG - Chr6:112872276-112872295 MsG0680035825.01.T01:CDS 45.0%
! GTGTCATTGGTTGTGTCAGA+AGG - Chr6:112871979-112871998 MsG0680035825.01.T01:CDS 45.0%
!! AGCTTGGTGTGAAGGAATCA+TGG - Chr6:112872221-112872240 MsG0680035825.01.T01:CDS 45.0%
!! CAGTGCTGACTGTTAAGATC+TGG + Chr6:112871821-112871840 None:intergenic 45.0%
AACCGAAACTTGGAGGAAGG+AGG + Chr6:112871684-112871703 None:intergenic 50.0%
CCTATTGGAGTGGGTACGAA+GGG - Chr6:112872286-112872305 MsG0680035825.01.T01:CDS 50.0%
CTATTGGAGTGGGTACGAAG+GGG - Chr6:112872287-112872306 MsG0680035825.01.T01:CDS 50.0%
GCCTAAGAAGTAACTCGTGG+AGG - Chr6:112871888-112871907 MsG0680035825.01.T01:CDS 50.0%
GGACGTTCAACACAAGGCAA+TGG + Chr6:112872268-112872287 None:intergenic 50.0%
GGAGCTCATGTCTTCATGGA+TGG - Chr6:112871953-112871972 MsG0680035825.01.T01:CDS 50.0%
TATTGGAGTGGGTACGAAGG+GGG - Chr6:112872288-112872307 MsG0680035825.01.T01:CDS 50.0%
TCCTATTGGAGTGGGTACGA+AGG - Chr6:112872285-112872304 MsG0680035825.01.T01:CDS 50.0%
TGAGGGAGCTCATGTCTTCA+TGG - Chr6:112871949-112871968 MsG0680035825.01.T01:CDS 50.0%
!! TATGGGTGAGCTTGGTGTGA+AGG - Chr6:112872213-112872232 MsG0680035825.01.T01:CDS 50.0%
AGTCAGCACTGCAGATGCCT+GGG - Chr6:112871830-112871849 MsG0680035825.01.T01:CDS 55.0%
CCCTTCGTACCCACTCCAAT+AGG + Chr6:112872289-112872308 None:intergenic 55.0%
CAGTCAGCACTGCAGATGCC+TGG - Chr6:112871829-112871848 MsG0680035825.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr6 gene 112871647 112872462 112871647 ID=MsG0680035825.01;Name=MsG0680035825.01
Chr6 mRNA 112871647 112872462 112871647 ID=MsG0680035825.01.T01;Parent=MsG0680035825.01;Name=MsG0680035825.01.T01;_AED=0.39;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|271
Chr6 exon 112871647 112872462 112871647 ID=MsG0680035825.01.T01:exon:9765;Parent=MsG0680035825.01.T01
Chr6 CDS 112871647 112872462 112871647 ID=MsG0680035825.01.T01:cds;Parent=MsG0680035825.01.T01
Gene Sequence

>MsG0680035825.01.T01

ATGTATTATGGCACCCATCTACTCAGAAATACAACCTCCTTCCTCCAAGTTTCGGTTGATTCATATGTCCCGGATGATGTTAAGCGTTCTTTTACCTTTGTGTCTTATCTTCATGGATTTGGTTATGACCGTGTTGCAAATGACTGTAAAGTCATTCGTTATTTATTCTTTCCAGATCTTAACAGTCAGCACTGCAGATGCCTGGGAAACAAATATTTCGAACCCTTATGGGAGATATATAGCCTAAGAAGTAACTCGTGGAGGAAACTTGATGTTGAAATGCCGCCTTCTTTCGACTATAGTGAGGGAGCTCATGTCTTCATGGATGGAGTGTGTCATTGGTTGTGTCAGAAGGATTATGGATATTGGAAAAAACATAATATCCCATTTCAACCTAGTTTGGTATCGTTTTACTTGAGCAACGAAGTGTTCTTCATAACACCCATACCCTCAGATGTAGATGATTGTTTTGATGTTGAAACAAATTGGAGAAACTTGGCAGTGCTAAATGGGACCATTGCATTGATCTCATATTATGAAAAGACGACTACTTTTCACGTATCAATTATGGGTGAGCTTGGTGTGAAGGAATCATGGATCAATCTCTTCACTATTGGACCATTGCCTTGTGTTGAACGTCCTATTGGAGTGGGTACGAAGGGGGAAATATTCTTCATAAGAAAAGATGAAGAACTAGCTTGGTTTGATTTGAGTACCCAAATGATTGAGGAGCTTGGTTTTAAAGTGGATGAACCTGAATGTCGGATAACAATTTACAAGGAAAGCATTCTTCCACTTGAAGGAATAAATAATTAA
Protein sequence

>MsG0680035825.01.T01

MYYGTHLLRNTTSFLQVSVDSYVPDDVKRSFTFVSYLHGFGYDRVANDCKVIRYLFFPDLNSQHCRCLGNKYFEPLWEIYSLRSNSWRKLDVEMPPSFDYSEGAHVFMDGVCHWLCQKDYGYWKKHNIPFQPSLVSFYLSNEVFFITPIPSDVDDCFDVETNWRNLAVLNGTIALISYYEKTTTFHVSIMGELGVKESWINLFTIGPLPCVERPIGVGTKGEIFFIRKDEELAWFDLSTQMIEELGFKVDEPECRITIYKESILPLEGINN*