AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029569.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029569.01.T01 MTR_8g012090 61.328 256 70 8 1 235 1 248 1.24e-92 277
MsG0580029569.01.T01 MTR_5g097330 51.211 289 83 7 1 234 1 286 7.12e-83 254
MsG0580029569.01.T01 MTR_7g111700 46.048 291 61 8 1 235 1 251 7.30e-65 207
MsG0580029569.01.T01 MTR_1g076810 51.293 232 72 8 8 198 10 241 7.81e-60 201
MsG0580029569.01.T01 MTR_7g104710 42.578 256 125 6 1 235 1 255 1.16e-54 179
MsG0580029569.01.T01 MTR_5g097350 43.929 280 106 8 1 233 1 276 2.70e-53 177
MsG0580029569.01.T01 MTR_0129s0120 40.559 286 110 8 1 234 2 279 4.97e-51 172
MsG0580029569.01.T01 MTR_7g017130 39.928 278 118 6 2 232 4 279 1.41e-50 171
MsG0580029569.01.T01 MTR_7g017170 39.928 278 118 6 2 232 4 279 1.49e-50 171
MsG0580029569.01.T01 MTR_0129s0070 40.559 286 110 8 1 234 2 279 1.79e-50 171
MsG0580029569.01.T01 MTR_8g039500 40.602 266 110 10 5 231 1 257 6.94e-48 162
MsG0580029569.01.T01 MTR_2g066170 38.235 272 117 9 5 231 1 266 8.13e-46 158
MsG0580029569.01.T01 MTR_4g028510 38.462 286 122 11 3 235 2 286 1.12e-45 159
MsG0580029569.01.T01 MTR_2g021800 35.762 302 127 9 1 235 1 302 2.81e-45 157
MsG0580029569.01.T01 MTR_8g063440 38.376 271 125 6 5 235 1 269 2.92e-45 157
MsG0580029569.01.T01 MTR_2g022000 36.877 301 124 11 1 235 1 301 3.46e-45 157
MsG0580029569.01.T01 MTR_5g096630 38.328 287 119 9 4 235 6 289 9.15e-45 156
MsG0580029569.01.T01 MTR_2g066200 36.264 273 133 7 5 236 1 273 1.05e-44 155
MsG0580029569.01.T01 MTR_6g080680 39.298 285 117 10 1 235 1 279 1.09e-44 155
MsG0580029569.01.T01 MTR_4g102410 36.395 294 123 8 1 235 44 332 1.30e-44 156
MsG0580029569.01.T01 MTR_4g028660 38.163 283 124 11 3 235 2 283 1.93e-44 155
MsG0580029569.01.T01 MTR_4g027450 38.163 283 124 11 3 235 2 283 1.93e-44 155
MsG0580029569.01.T01 MTR_7g017040 37.367 281 124 6 1 231 1 279 2.23e-44 155
MsG0580029569.01.T01 MTR_5g037680 37.546 269 118 7 11 235 3 265 2.59e-44 155
MsG0580029569.01.T01 MTR_2g065860 36.630 273 129 8 5 235 1 271 3.49e-44 154
MsG0580029569.01.T01 MTR_1g007450 37.770 278 122 5 5 232 1 277 1.40e-43 153
MsG0580029569.01.T01 MTR_2g021950 37.143 280 125 9 1 231 1 278 1.69e-43 152
MsG0580029569.01.T01 MTR_7g079640 38.610 259 128 7 1 234 1 253 5.10e-43 150
MsG0580029569.01.T01 MTR_1g007410 35.986 289 128 8 1 235 7 292 7.70e-43 150
MsG0580029569.01.T01 MTR_2g021770 35.640 289 119 8 10 235 6 290 8.48e-43 150
MsG0580029569.01.T01 MTR_2g065840 36.957 276 123 10 5 234 1 271 1.15e-42 150
MsG0580029569.01.T01 MTR_4g028680 38.028 284 124 11 3 235 2 284 1.17e-41 147
MsG0580029569.01.T01 MTR_4g130330 39.273 275 107 11 15 235 6 274 1.72e-41 147
MsG0580029569.01.T01 MTR_3g011120 38.007 271 117 10 5 231 1 264 2.12e-41 146
MsG0580029569.01.T01 MTR_7g016930 36.630 273 112 8 1 231 1 254 2.55e-41 145
MsG0580029569.01.T01 MTR_6g086825 36.396 283 113 7 14 235 68 344 3.20e-41 147
MsG0580029569.01.T01 MTR_2g066150 36.897 290 150 10 5 270 1 281 3.27e-41 145
MsG0580029569.01.T01 MTR_8g041270 35.959 292 121 9 10 242 9 293 4.29e-41 146
MsG0580029569.01.T01 MTR_8g046310 38.636 264 122 9 6 233 3 262 7.44e-41 145
MsG0580029569.01.T01 MTR_8g036970 40.278 216 108 8 24 230 6 209 9.03e-41 144
MsG0580029569.01.T01 MTR_8g464770 36.861 274 126 8 5 237 1 268 2.04e-40 144
MsG0580029569.01.T01 MTR_5g091140 38.202 267 126 10 5 236 37 299 2.99e-40 144
MsG0580029569.01.T01 MTR_2g064950 35.424 271 127 9 7 231 1 269 4.12e-40 143
MsG0580029569.01.T01 MTR_8g046220 35.461 282 116 9 13 233 14 290 5.13e-40 143
MsG0580029569.01.T01 MTR_5g037190 39.921 253 117 8 1 221 1 250 6.04e-40 139
MsG0580029569.01.T01 MTR_2g104340 36.332 289 123 10 6 242 4 283 1.31e-39 142
MsG0580029569.01.T01 MTR_5g097220 36.012 336 135 13 1 268 1 324 5.05e-39 140
MsG0580029569.01.T01 MTR_2g034700 36.090 266 131 8 8 236 5 268 6.08e-39 140
MsG0580029569.01.T01 MTR_4g014370 37.860 243 116 7 1 233 8 225 1.62e-38 138
MsG0580029569.01.T01 MTR_8g064890 30.769 312 153 9 14 273 14 314 2.43e-38 139
MsG0580029569.01.T01 MTR_6g465360 52.985 134 61 1 2 133 48 181 6.11e-38 135
MsG0580029569.01.T01 MTR_1g053510 32.727 330 145 12 4 268 2 319 1.26e-37 137
MsG0580029569.01.T01 MTR_5g024260 37.743 257 118 8 15 232 21 274 2.02e-37 137
MsG0580029569.01.T01 MTR_5g096910 38.321 274 117 9 6 232 7 275 2.21e-37 136
MsG0580029569.01.T01 MTR_5g069390 35.667 300 119 11 1 231 1 295 5.17e-37 137
MsG0580029569.01.T01 MTR_5g097820 35.849 265 117 8 5 219 8 269 5.72e-37 132
MsG0580029569.01.T01 MTR_8g063330 34.532 278 129 7 5 235 1 272 9.37e-37 136
MsG0580029569.01.T01 MTR_5g094350 34.799 273 123 7 12 235 9 275 1.79e-36 131
MsG0580029569.01.T01 MTR_5g096900 35.036 274 131 7 11 240 7 277 2.76e-36 132
MsG0580029569.01.T01 MTR_2g104280 35.017 297 123 9 7 242 5 292 9.45e-36 132
MsG0580029569.01.T01 MTR_6g079770 52.239 134 62 1 2 133 3 136 2.26e-35 131
MsG0580029569.01.T01 MTR_6g079770 54.667 75 30 2 165 235 227 301 1.59e-16 79.3
MsG0580029569.01.T01 MTR_1g050308 38.813 219 98 5 1 184 19 236 1.28e-34 128
MsG0580029569.01.T01 MTR_5g059430 33.798 287 126 8 7 231 17 301 2.07e-34 129
MsG0580029569.01.T01 MTR_0134s0060 54.198 131 58 1 5 133 1 131 2.09e-34 129
MsG0580029569.01.T01 MTR_0134s0060 56.579 76 29 2 164 235 221 296 4.19e-17 80.9
MsG0580029569.01.T01 MTR_6g478230 35.185 324 141 12 1 267 1 312 5.06e-34 127
MsG0580029569.01.T01 MTR_0831s0010 36.260 262 115 8 13 231 47 299 5.39e-34 127
MsG0580029569.01.T01 MTR_0054s0090 53.125 128 58 1 2 127 3 130 7.99e-34 127
MsG0580029569.01.T01 MTR_0054s0090 54.118 85 36 2 150 233 206 288 4.57e-18 83.6
MsG0580029569.01.T01 MTR_1g007430 35.433 254 107 7 27 234 7 249 3.41e-33 124
MsG0580029569.01.T01 MTR_6g070880 51.515 132 59 3 1 128 15 145 4.97e-33 122
MsG0580029569.01.T01 MTR_6g071420 51.515 132 59 3 1 128 15 145 4.97e-33 122
MsG0580029569.01.T01 MTR_6g079810 34.333 300 126 10 1 235 54 347 8.81e-33 125
MsG0580029569.01.T01 MTR_5g074850 35.034 294 121 11 1 232 20 305 1.95e-32 126
MsG0580029569.01.T01 MTR_0054s0100 51.969 127 59 1 3 127 4 130 2.71e-32 123
MsG0580029569.01.T01 MTR_0054s0100 54.118 85 36 2 150 233 208 290 4.90e-18 83.6
MsG0580029569.01.T01 MTR_6g461660 52.308 130 57 2 2 127 5 133 1.34e-31 120
MsG0580029569.01.T01 MTR_6g461660 56.944 72 26 2 165 235 218 285 4.41e-17 80.5
MsG0580029569.01.T01 MTR_2g038680 48.872 133 62 2 1 127 1 133 4.69e-31 119
MsG0580029569.01.T01 MTR_2g038680 48.052 77 26 3 166 234 228 298 3.79e-12 66.2
MsG0580029569.01.T01 MTR_1g115205 36.100 241 94 10 1 203 17 235 5.68e-31 116
MsG0580029569.01.T01 MTR_5g072060 33.463 257 136 8 14 239 4 256 6.41e-31 121
MsG0580029569.01.T01 MTR_4g117550 51.515 132 57 4 1 126 1 131 2.77e-30 117
MsG0580029569.01.T01 MTR_4g117550 52.703 74 24 4 166 234 213 280 3.79e-14 72.0
MsG0580029569.01.T01 MTR_8g467670 32.700 263 132 7 9 231 6 263 3.13e-30 117
MsG0580029569.01.T01 MTR_5g023970 75.000 72 17 1 165 235 9 80 1.05e-29 111
MsG0580029569.01.T01 MTR_8g064790 32.830 265 159 9 14 273 13 263 2.37e-29 114
MsG0580029569.01.T01 MTR_8g013000 31.481 270 130 7 7 225 1 266 4.06e-29 114
MsG0580029569.01.T01 MTR_8g033140 50.376 133 59 4 1 126 1 133 4.21e-29 114
MsG0580029569.01.T01 MTR_7g023800 36.410 195 106 4 55 233 1 193 5.74e-29 112
MsG0580029569.01.T01 MTR_0129s0050 48.120 133 61 3 1 130 1 128 7.82e-29 113
MsG0580029569.01.T01 MTR_0129s0050 42.391 92 51 2 153 242 209 300 5.63e-14 71.6
MsG0580029569.01.T01 MTR_4g055820 51.562 128 54 4 6 127 4 129 8.25e-29 112
MsG0580029569.01.T01 MTR_4g055820 43.023 86 48 1 158 242 205 290 2.85e-16 78.2
MsG0580029569.01.T01 MTR_5g097680 53.636 110 48 1 2 108 3 112 1.42e-28 113
MsG0580029569.01.T01 MTR_5g097680 44.156 77 37 2 165 235 237 313 6.92e-12 65.5
MsG0580029569.01.T01 MTR_6g078010 35.023 217 105 6 53 233 1 217 1.67e-27 108
MsG0580029569.01.T01 MTR_8g032980 54.701 117 43 4 15 127 15 125 3.76e-27 109
MsG0580029569.01.T01 MTR_5g075370 34.043 282 115 12 15 232 7 281 8.97e-27 109
MsG0580029569.01.T01 MTR_7g069550 44.444 135 64 4 1 127 1 132 1.03e-26 107
MsG0580029569.01.T01 MTR_7g069550 43.590 78 38 3 166 242 215 287 3.01e-12 66.6
MsG0580029569.01.T01 MTR_8g063370 30.769 273 145 8 24 257 32 299 1.39e-26 107
MsG0580029569.01.T01 MTR_6g090180 44.615 130 69 2 3 129 34 163 2.27e-26 107
MsG0580029569.01.T01 MTR_6g090180 51.389 72 34 1 165 235 251 322 9.24e-15 73.9
MsG0580029569.01.T01 MTR_5g092850 31.900 279 122 8 15 232 15 286 2.51e-26 106
MsG0580029569.01.T01 MTR_8g033110 30.952 294 130 10 5 232 1 287 6.21e-26 105
MsG0580029569.01.T01 MTR_4g028750 51.163 129 56 5 3 126 2 128 7.61e-26 105
MsG0580029569.01.T01 MTR_4g028750 46.667 75 36 3 165 235 210 284 1.51e-11 64.3
MsG0580029569.01.T01 MTR_8g041440 53.398 103 31 2 1 103 1 86 1.58e-25 97.8
MsG0580029569.01.T01 MTR_1g098180 32.787 244 120 8 13 231 16 240 7.24e-25 102
MsG0580029569.01.T01 MTR_5g092860 31.923 260 122 8 35 245 21 274 7.99e-25 102
MsG0580029569.01.T01 MTR_8g073570 42.623 122 66 2 7 126 1 120 8.21e-25 97.8
MsG0580029569.01.T01 MTR_8g046200 46.043 139 53 5 6 126 3 137 7.48e-24 100
MsG0580029569.01.T01 MTR_8g046200 43.836 73 36 2 165 235 240 309 5.49e-11 62.8
MsG0580029569.01.T01 MTR_8g018110 30.405 296 128 11 5 231 1 287 1.70e-23 99.0
MsG0580029569.01.T01 MTR_5g097650 47.154 123 50 4 9 126 13 125 2.10e-23 93.6
MsG0580029569.01.T01 MTR_2g104260 33.333 279 122 9 6 226 4 276 3.38e-23 96.3
MsG0580029569.01.T01 MTR_5g022850 28.438 320 127 10 1 268 1 270 1.78e-22 95.5
MsG0580029569.01.T01 MTR_5g076490 32.377 244 104 9 53 251 1 228 3.00e-22 94.7
MsG0580029569.01.T01 MTR_4g014370 44.186 129 67 3 9 132 4 132 9.59e-22 94.0
MsG0580029569.01.T01 MTR_5g074800 29.752 242 112 10 55 247 1 233 2.01e-21 92.4
MsG0580029569.01.T01 MTR_5g097540 33.043 230 85 10 53 265 1 178 3.56e-20 88.2
MsG0580029569.01.T01 MTR_4g007940 28.837 215 104 7 66 235 4 214 2.88e-19 86.3
MsG0580029569.01.T01 MTR_3g019290 34.946 186 96 8 5 173 1 178 1.26e-18 81.6
MsG0580029569.01.T01 MTR_8g063350 25.080 311 146 11 8 273 6 274 1.82e-18 84.0
MsG0580029569.01.T01 MTR_5g096070 42.336 137 41 6 7 134 14 121 4.82e-18 79.3
MsG0580029569.01.T01 MTR_8g033090 43.966 116 60 3 15 126 13 127 1.05e-17 82.4
MsG0580029569.01.T01 MTR_5g043605 31.884 207 101 8 87 256 45 248 1.44e-17 81.6
MsG0580029569.01.T01 MTR_8g063310 24.904 261 152 10 7 229 13 267 2.18e-17 81.6
MsG0580029569.01.T01 MTR_6g090250 46.875 96 46 4 36 127 31 125 2.32e-16 78.6
MsG0580029569.01.T01 MTR_3g047580 35.772 123 53 3 10 128 7 107 4.11e-16 73.6
MsG0580029569.01.T01 MTR_7g101200 29.469 207 96 5 30 231 1 162 1.68e-15 74.3
MsG0580029569.01.T01 MTR_3g047540 47.059 68 34 1 164 231 3 68 2.72e-15 70.1
MsG0580029569.01.T01 MTR_5g064700 48.718 78 32 2 165 234 176 253 6.08e-15 74.3
MsG0580029569.01.T01 MTR_5g086780 61.290 62 22 1 1 60 1 62 8.61e-15 72.0
MsG0580029569.01.T01 MTR_5g086590 52.239 67 29 2 166 232 77 140 1.13e-14 72.0
MsG0580029569.01.T01 MTR_5g068490 32.804 189 92 7 95 256 8 188 3.31e-14 71.2
MsG0580029569.01.T01 MTR_6g004450 50.000 76 28 3 165 234 86 157 1.05e-13 69.7
MsG0580029569.01.T01 MTR_6g090260 50.000 74 34 2 165 235 73 146 1.19e-13 69.3
MsG0580029569.01.T01 MTR_6g090260 55.072 69 27 2 1 65 1 69 1.62e-12 66.2
MsG0580029569.01.T01 MTR_3g018750 42.222 90 30 4 159 231 40 124 2.93e-13 68.2
MsG0580029569.01.T01 MTR_6g087660 49.351 77 36 2 2 75 3 79 1.72e-12 65.1
MsG0580029569.01.T01 MTR_6g009350 42.683 82 41 3 159 234 39 120 1.22e-11 62.8
MsG0580029569.01.T01 MTR_7g017150 45.000 80 39 3 159 235 74 151 1.51e-11 63.5
MsG0580029569.01.T01 MTR_2g021920 41.772 79 41 2 158 231 76 154 1.87e-11 63.2
MsG0580029569.01.T01 MTR_7g017120 44.304 79 39 3 160 235 74 150 6.02e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029569.01.T01 AT3G22730 28.163 245 137 10 19 236 9 241 5.64e-11 62.8

Find 59 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTAACAAATTCATGAAATA+AGG 0.114074 5:-98690074 None:intergenic
ATTCATTGTATTGAAGAATT+AGG 0.176108 5:+98690326 MsG0580029569.01.T01:CDS
ATTCTATCTTCATAAGTTCT+AGG 0.205762 5:-98690536 None:intergenic
AGAATAAGGTTAAATTAAAT+TGG 0.213096 5:+98690210 MsG0580029569.01.T01:CDS
CCTGTGTTGTATTCTCTTCT+TGG 0.213598 5:+98690176 MsG0580029569.01.T01:CDS
ACGAAAGAATTTCTTATCTA+AGG 0.230349 5:+98690094 MsG0580029569.01.T01:CDS
CAATGTTCTGCTAGTGTTAA+TGG 0.244446 5:+98690269 MsG0580029569.01.T01:CDS
AACATGGATAATAAAGTTTC+CGG 0.265249 5:+98690827 MsG0580029569.01.T01:CDS
ATTTGTAATCCATATGATTC+AGG 0.279368 5:-98690148 None:intergenic
GCCTTTACAATACTATGGTT+TGG 0.296302 5:+98690646 MsG0580029569.01.T01:CDS
AATTGTTCATCATATGGATT+TGG 0.305705 5:-98690233 None:intergenic
TTCATTGTATTGAAGAATTA+GGG 0.312151 5:+98690327 MsG0580029569.01.T01:CDS
TGGCGAGAGATTCGAGAATA+AGG 0.313737 5:+98690196 MsG0580029569.01.T01:CDS
TAGGCAATGTCTTGTGATTC+CGG 0.320830 5:-98690846 None:intergenic
CCTTTACAATACTATGGTTT+GGG 0.321447 5:+98690647 MsG0580029569.01.T01:CDS
GACATGCCTTTACAATACTA+TGG 0.322238 5:+98690641 MsG0580029569.01.T01:CDS
AAATCAAATTGTTCATCATA+TGG 0.336239 5:-98690239 None:intergenic
TAAGGATCATTCTTATTATG+AGG 0.362501 5:+98690112 MsG0580029569.01.T01:CDS
GTAACAAATTCATGAAATAA+GGG 0.374764 5:-98690073 None:intergenic
CACCTCCATGTTTGAGGGTA+TGG 0.375586 5:-98690780 None:intergenic
AACGTGTGATAATGTTTATT+TGG 0.388714 5:+98690718 MsG0580029569.01.T01:CDS
CTCTTCACTAATGTCAATAG+TGG 0.403231 5:-98691159 None:intergenic
ATCTCTCCTCCTGAATCATA+TGG 0.428722 5:+98690139 MsG0580029569.01.T01:CDS
AATCACAAGACATTGCCTAT+TGG 0.432155 5:+98690850 MsG0580029569.01.T01:CDS
AACTTTATTATCCATGTTTG+AGG 0.435142 5:-98690822 None:intergenic
CCCTTCTTACCAACTCCAAT+AGG 0.452976 5:-98690865 None:intergenic
TTTAGAGATGGAGAAATCAT+TGG 0.461768 5:+98689920 None:intergenic
TCTAGGATGACAATCTTCTT+CGG 0.463534 5:-98690519 None:intergenic
AACATACACCTCCATGTTTG+AGG 0.475209 5:-98690786 None:intergenic
ATTCTTTGTATTCAAGATGT+GGG 0.491423 5:+98690293 MsG0580029569.01.T01:CDS
ATTGGCCGTAACAACTGAAA+AGG 0.499447 5:+98689938 MsG0580029569.01.T01:CDS
TATTCAAGATGTGGGGAGAA+GGG 0.507909 5:+98690301 MsG0580029569.01.T01:CDS
GTATTCAAGATGTGGGGAGA+AGG 0.515024 5:+98690300 MsG0580029569.01.T01:CDS
CCCAAACCATAGTATTGTAA+AGG 0.517915 5:-98690647 None:intergenic
ACACAGATGGAGTGTGTCAC+TGG 0.521115 5:+98690681 MsG0580029569.01.T01:CDS
AAGACATTGCCTATTGGAGT+TGG 0.530015 5:+98690856 MsG0580029569.01.T01:CDS
TATTCTTTGTATTCAAGATG+TGG 0.533134 5:+98690292 MsG0580029569.01.T01:CDS
ACATACACCTCCATGTTTGA+GGG 0.540694 5:-98690785 None:intergenic
AAGGATCATTCTTATTATGA+GGG 0.541585 5:+98690113 MsG0580029569.01.T01:CDS
TAGACACCGTCGCCCCTTGT+GGG 0.554634 5:+98690574 MsG0580029569.01.T01:CDS
CACTATTGACATTAGTGAAG+AGG 0.555530 5:+98691160 MsG0580029569.01.T01:CDS
ACTTTATTATCCATGTTTGA+GGG 0.557560 5:-98690821 None:intergenic
GGTGTTCAAGTGTACACAGA+TGG 0.572148 5:+98690668 MsG0580029569.01.T01:CDS
ATAGACACCGTCGCCCCTTG+TGG 0.575699 5:+98690573 MsG0580029569.01.T01:CDS
TTCTTTGTATTCAAGATGTG+GGG 0.584238 5:+98690294 MsG0580029569.01.T01:CDS
AACATCAATACCCTCAAACA+TGG 0.587220 5:+98690811 MsG0580029569.01.T01:CDS
ATATCTCCCACAAGGGGCGA+CGG 0.594720 5:-98690580 None:intergenic
AACATCCATACCCTCAAACA+TGG 0.608765 5:+98690775 MsG0580029569.01.T01:CDS
ATTCAAGATGTGGGGAGAAG+GGG 0.611107 5:+98690302 MsG0580029569.01.T01:CDS
AATCTAAACAAAGATTTGAG+AGG 0.611998 5:-98690011 None:intergenic
TAGACAATATATCTCCCACA+AGG 0.624339 5:-98690588 None:intergenic
TTTGTTGTGCGAATAAATCG+TGG 0.627762 5:+98690033 MsG0580029569.01.T01:CDS
TGTAATCCATATGATTCAGG+AGG 0.638814 5:-98690145 None:intergenic
ATGAAGATAGAATAAAGATG+TGG 0.649467 5:+98690546 MsG0580029569.01.T01:CDS
GACAATATATCTCCCACAAG+GGG 0.658931 5:-98690586 None:intergenic
AGACAATATATCTCCCACAA+GGG 0.670595 5:-98690587 None:intergenic
CAGATGGAGTGTGTCACTGG+CGG 0.676705 5:+98690684 MsG0580029569.01.T01:CDS
CCAAGAAGAGAATACAACAC+AGG 0.708880 5:-98690176 None:intergenic
ATCCATACCCTCAAACATGG+AGG 0.738802 5:+98690778 MsG0580029569.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAATAAGGTTAAATTAAAT+TGG + Chr5:98690210-98690229 MsG0580029569.01.T01:CDS 15.0%
!! AAATCAAATTGTTCATCATA+TGG - Chr5:98690242-98690261 None:intergenic 20.0%
!! AATCCATGAATAGTAATATA+TGG - Chr5:98690437-98690456 None:intergenic 20.0%
!! ATTCATTGTATTGAAGAATT+AGG + Chr5:98690326-98690345 MsG0580029569.01.T01:CDS 20.0%
!! GTAACAAATTCATGAAATAA+GGG - Chr5:98690076-98690095 None:intergenic 20.0%
!! TATATTACTATTCATGGATT+TGG + Chr5:98690437-98690456 MsG0580029569.01.T01:intron 20.0%
!! TTCATTGTATTGAAGAATTA+GGG + Chr5:98690327-98690346 MsG0580029569.01.T01:CDS 20.0%
!!! TTTACTTTTTTTGAGCATAT+TGG + Chr5:98691124-98691143 MsG0580029569.01.T01:intron 20.0%
! AACATGGATAATAAAGTTTC+CGG + Chr5:98690827-98690846 MsG0580029569.01.T01:CDS 25.0%
! AACTTTATTATCCATGTTTG+AGG - Chr5:98690825-98690844 None:intergenic 25.0%
! AAGGATCATTCTTATTATGA+GGG + Chr5:98690113-98690132 MsG0580029569.01.T01:CDS 25.0%
! AATCTAAACAAAGATTTGAG+AGG - Chr5:98690014-98690033 None:intergenic 25.0%
! AATTGTTCATCATATGGATT+TGG - Chr5:98690236-98690255 None:intergenic 25.0%
! ACGAAAGAATTTCTTATCTA+AGG + Chr5:98690094-98690113 MsG0580029569.01.T01:CDS 25.0%
! ACTTTATTATCCATGTTTGA+GGG - Chr5:98690824-98690843 None:intergenic 25.0%
! ATGAAGATAGAATAAAGATG+TGG + Chr5:98690546-98690565 MsG0580029569.01.T01:CDS 25.0%
! ATTCTATCTTCATAAGTTCT+AGG - Chr5:98690539-98690558 None:intergenic 25.0%
! ATTCTTTGTATTCAAGATGT+GGG + Chr5:98690293-98690312 MsG0580029569.01.T01:CDS 25.0%
! ATTGTCTAAAAAGTAACTCT+TGG + Chr5:98690603-98690622 MsG0580029569.01.T01:CDS 25.0%
! ATTTGTAATCCATATGATTC+AGG - Chr5:98690151-98690170 None:intergenic 25.0%
! CGTAACAAATTCATGAAATA+AGG - Chr5:98690077-98690096 None:intergenic 25.0%
! GATCCATATATTACTATTCA+TGG + Chr5:98690431-98690450 MsG0580029569.01.T01:intron 25.0%
! GTAATATATGGATCTCAATA+AGG - Chr5:98690425-98690444 None:intergenic 25.0%
! TAAGGATCATTCTTATTATG+AGG + Chr5:98690112-98690131 MsG0580029569.01.T01:CDS 25.0%
! TATTCTTTGTATTCAAGATG+TGG + Chr5:98690292-98690311 MsG0580029569.01.T01:CDS 25.0%
!! AACGTGTGATAATGTTTATT+TGG + Chr5:98690718-98690737 MsG0580029569.01.T01:CDS 25.0%
!!! AAGTTTTGATAGAATGACTA+AGG - Chr5:98689991-98690010 None:intergenic 25.0%
!!! CGATATCTTTTTCATGAAAA+AGG + Chr5:98690888-98690907 MsG0580029569.01.T01:intron 25.0%
!!! CTTTTTCATGAAAAAGGATA+GGG + Chr5:98690894-98690913 MsG0580029569.01.T01:intron 25.0%
!!! TCTTTTTCATGAAAAAGGAT+AGG + Chr5:98690893-98690912 MsG0580029569.01.T01:intron 25.0%
!!! TTTTTTTGAGCATATTGGTA+TGG + Chr5:98691129-98691148 MsG0580029569.01.T01:intron 25.0%
ACAAAAAGAATAGAAGACCT+TGG + Chr5:98690940-98690959 MsG0580029569.01.T01:intron 30.0%
ATAGAAGACCTTGGTATTAA+AGG + Chr5:98690949-98690968 MsG0580029569.01.T01:intron 30.0%
CCTTTACAATACTATGGTTT+GGG + Chr5:98690647-98690666 MsG0580029569.01.T01:CDS 30.0%
TAAGGAGATTCAAATGCAAA+AGG - Chr5:98690407-98690426 None:intergenic 30.0%
TTCTTTGTATTCAAGATGTG+GGG + Chr5:98690294-98690313 MsG0580029569.01.T01:CDS 30.0%
! GTTAACCTTTTCAGTTGTTA+CGG - Chr5:98689946-98689965 None:intergenic 30.0%
AACATCAATACCCTCAAACA+TGG + Chr5:98690811-98690830 MsG0580029569.01.T01:CDS 35.0%
AATCACAAGACATTGCCTAT+TGG + Chr5:98690850-98690869 MsG0580029569.01.T01:CDS 35.0%
AGACAATATATCTCCCACAA+GGG - Chr5:98690590-98690609 None:intergenic 35.0%
CAATGTTCTGCTAGTGTTAA+TGG + Chr5:98690269-98690288 MsG0580029569.01.T01:CDS 35.0%
CACTATTGACATTAGTGAAG+AGG + Chr5:98691160-98691179 MsG0580029569.01.T01:CDS 35.0%
CCAAATTGGAGATGACTATA+AGG + Chr5:98690466-98690485 MsG0580029569.01.T01:intron 35.0%
CCCAAACCATAGTATTGTAA+AGG - Chr5:98690650-98690669 None:intergenic 35.0%
CCTTATAGTCATCTCCAATT+TGG - Chr5:98690469-98690488 None:intergenic 35.0%
CTCTTCACTAATGTCAATAG+TGG - Chr5:98691162-98691181 None:intergenic 35.0%
GACATGCCTTTACAATACTA+TGG + Chr5:98690641-98690660 MsG0580029569.01.T01:CDS 35.0%
GCCTTTACAATACTATGGTT+TGG + Chr5:98690646-98690665 MsG0580029569.01.T01:CDS 35.0%
GTCTTTCACCTTTAATACCA+AGG - Chr5:98690960-98690979 None:intergenic 35.0%
TAGACAATATATCTCCCACA+AGG - Chr5:98690591-98690610 None:intergenic 35.0%
TGTAATCCATATGATTCAGG+AGG - Chr5:98690148-98690167 None:intergenic 35.0%
! GGATTTGGTTACAACCAAAT+TGG + Chr5:98690452-98690471 MsG0580029569.01.T01:intron 35.0%
! TCAAATGCAAAAGGACTAGT+AGG - Chr5:98690398-98690417 None:intergenic 35.0%
! TCTAGGATGACAATCTTCTT+CGG - Chr5:98690522-98690541 None:intergenic 35.0%
! TTTGTTGTGCGAATAAATCG+TGG + Chr5:98690033-98690052 MsG0580029569.01.T01:CDS 35.0%
AACATACACCTCCATGTTTG+AGG - Chr5:98690789-98690808 None:intergenic 40.0%
AACATCCATACCCTCAAACA+TGG + Chr5:98690775-98690794 MsG0580029569.01.T01:CDS 40.0%
AAGACATTGCCTATTGGAGT+TGG + Chr5:98690856-98690875 MsG0580029569.01.T01:CDS 40.0%
AATTAGGGTGAGTTGTGTTG+TGG + Chr5:98690342-98690361 MsG0580029569.01.T01:intron 40.0%
ACATACACCTCCATGTTTGA+GGG - Chr5:98690788-98690807 None:intergenic 40.0%
ACTTTGAATTCTCCGGTAGA+AGG - Chr5:98690371-98690390 None:intergenic 40.0%
AGGAGTGACTTTGAATTCTC+CGG - Chr5:98690378-98690397 None:intergenic 40.0%
ATCTCTCCTCCTGAATCATA+TGG + Chr5:98690139-98690158 MsG0580029569.01.T01:CDS 40.0%
ATTGGCCGTAACAACTGAAA+AGG + Chr5:98689938-98689957 MsG0580029569.01.T01:CDS 40.0%
CCAAGAAGAGAATACAACAC+AGG - Chr5:98690179-98690198 None:intergenic 40.0%
CCTGTGTTGTATTCTCTTCT+TGG + Chr5:98690176-98690195 MsG0580029569.01.T01:CDS 40.0%
GACAATATATCTCCCACAAG+GGG - Chr5:98690589-98690608 None:intergenic 40.0%
TAGGCAATGTCTTGTGATTC+CGG - Chr5:98690849-98690868 None:intergenic 40.0%
TATTCAAGATGTGGGGAGAA+GGG + Chr5:98690301-98690320 MsG0580029569.01.T01:CDS 40.0%
TGACAATCTTCTTCGGTTCT+AGG - Chr5:98690515-98690534 None:intergenic 40.0%
!! CCTATTGGAGTTGGTAAGAA+GGG + Chr5:98690865-98690884 MsG0580029569.01.T01:intron 40.0%
ATCCATACCCTCAAACATGG+AGG + Chr5:98690778-98690797 MsG0580029569.01.T01:CDS 45.0%
ATTCAAGATGTGGGGAGAAG+GGG + Chr5:98690302-98690321 MsG0580029569.01.T01:CDS 45.0%
CCCTTCTTACCAACTCCAAT+AGG - Chr5:98690868-98690887 None:intergenic 45.0%
GGTGTTCAAGTGTACACAGA+TGG + Chr5:98690668-98690687 MsG0580029569.01.T01:CDS 45.0%
GTATTCAAGATGTGGGGAGA+AGG + Chr5:98690300-98690319 MsG0580029569.01.T01:CDS 45.0%
GTGTTGTGGAATCCTTCTAC+CGG + Chr5:98690356-98690375 MsG0580029569.01.T01:intron 45.0%
TGGCGAGAGATTCGAGAATA+AGG + Chr5:98690196-98690215 MsG0580029569.01.T01:CDS 45.0%
!! GCCTATTGGAGTTGGTAAGA+AGG + Chr5:98690864-98690883 MsG0580029569.01.T01:intron 45.0%
!! ATTATTTATAAACTATTATA+CGG - Chr5:98690987-98691006 None:intergenic 5.0%
ACACAGATGGAGTGTGTCAC+TGG + Chr5:98690681-98690700 MsG0580029569.01.T01:CDS 50.0%
CACCTCCATGTTTGAGGGTA+TGG - Chr5:98690783-98690802 None:intergenic 50.0%
ATATCTCCCACAAGGGGCGA+CGG - Chr5:98690583-98690602 None:intergenic 55.0%
! CAGATGGAGTGTGTCACTGG+CGG + Chr5:98690684-98690703 MsG0580029569.01.T01:CDS 55.0%
ATAGACACCGTCGCCCCTTG+TGG + Chr5:98690573-98690592 MsG0580029569.01.T01:CDS 60.0%
TAGACACCGTCGCCCCTTGT+GGG + Chr5:98690574-98690593 MsG0580029569.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 98689927 98691193 98689927 ID=MsG0580029569.01;Name=MsG0580029569.01
Chr5 mRNA 98689927 98691193 98689927 ID=MsG0580029569.01.T01;Parent=MsG0580029569.01;Name=MsG0580029569.01.T01;_AED=0.41;_eAED=0.43;_QI=0|0|0|0.66|1|1|3|0|273
Chr5 exon 98689927 98690348 98689927 ID=MsG0580029569.01.T01:exon:25843;Parent=MsG0580029569.01.T01
Chr5 exon 98690517 98690877 98690517 ID=MsG0580029569.01.T01:exon:25844;Parent=MsG0580029569.01.T01
Chr5 exon 98691155 98691193 98691155 ID=MsG0580029569.01.T01:exon:25845;Parent=MsG0580029569.01.T01
Chr5 CDS 98689927 98690348 98689927 ID=MsG0580029569.01.T01:cds;Parent=MsG0580029569.01.T01
Chr5 CDS 98690517 98690877 98690517 ID=MsG0580029569.01.T01:cds;Parent=MsG0580029569.01.T01
Chr5 CDS 98691155 98691193 98691155 ID=MsG0580029569.01.T01:cds;Parent=MsG0580029569.01.T01
Gene Sequence

>MsG0580029569.01.T01

ATGGAGAAATCATTGGCCGTAACAACTGAAAAGGTTAACGATCACATATATGATGATGTTGCCTTAGTCATTCTATCAAAACTTCCTCTCAAATCTTTGTTTAGATTTTGTTGTGCGAATAAATCGTGGTCTCTCTTATTTGAAAACCCTTATTTCATGAATTTGTTACGAAAGAATTTCTTATCTAAGGATCATTCTTATTATGAGGGTACATCTCTCCTCCTGAATCATATGGATTACAAATATAAACCTGTGTTGTATTCTCTTCTTGGCGAGAGATTCGAGAATAAGGTTAAATTAAATTGGCCAAATCCATATGATGAACAATTTGATTTTATTGTACAATGTTCTGCTAGTGTTAATGGTATTCTTTGTATTCAAGATGTGGGGAGAAGGGGCATTCATTGTATTGAAGAATTAGGAACCGAAGAAGATTGTCATCCTAGAACTTATGAAGATAGAATAAAGATGTGGAAAGATAGACACCGTCGCCCCTTGTGGGAGATATATTGTCTAAAAAGTAACTCTTGGAGAAAACTTGATATAGACATGCCTTTACAATACTATGGTTTGGGTGTTCAAGTGTACACAGATGGAGTGTGTCACTGGCGGCGTGAGAGTGAAACGTGTGATAATGTTTATTTGGTGTCATTTGACTTGAGCAACGAGACATTTGTTAGAACATCCATACCCTCAAACATGGAGGTGTATGTTGAAACATCAATACCCTCAAACATGGATAATAAAGTTTCCGGAATCACAAGACATTGCCTATTGGAGTTGTACACCACTATTGACATTAGTGAAGAGGAAACCAAATGA
Protein sequence

>MsG0580029569.01.T01

MEKSLAVTTEKVNDHIYDDVALVILSKLPLKSLFRFCCANKSWSLLFENPYFMNLLRKNFLSKDHSYYEGTSLLLNHMDYKYKPVLYSLLGERFENKVKLNWPNPYDEQFDFIVQCSASVNGILCIQDVGRRGIHCIEELGTEEDCHPRTYEDRIKMWKDRHRRPLWEIYCLKSNSWRKLDIDMPLQYYGLGVQVYTDGVCHWRRESETCDNVYLVSFDLSNETFVRTSIPSNMEVYVETSIPSNMDNKVSGITRHCLLELYTTIDISEEETK*