AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043307.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043307.01.T01 MTR_5g097220 44.856 243 107 5 37 258 6 242 1.95e-60 197
MsG0880043307.01.T01 MTR_0129s0070 49.558 226 96 6 37 250 10 229 1.61e-59 194
MsG0880043307.01.T01 MTR_0134s0060 46.862 239 104 5 35 250 3 241 2.19e-59 194
MsG0880043307.01.T01 MTR_4g117550 45.798 238 108 7 35 260 8 236 5.42e-58 189
MsG0880043307.01.T01 MTR_2g021800 44.015 259 105 8 36 263 8 257 1.88e-57 189
MsG0880043307.01.T01 MTR_0129s0050 47.200 250 98 7 32 254 2 244 2.95e-57 189
MsG0880043307.01.T01 MTR_0054s0090 44.726 237 114 5 30 250 3 238 5.34e-57 188
MsG0880043307.01.T01 MTR_8g012090 46.531 245 84 8 29 259 1 212 6.08e-57 186
MsG0880043307.01.T01 MTR_7g017040 50.226 221 94 8 39 250 11 224 8.73e-57 187
MsG0880043307.01.T01 MTR_2g022000 44.574 258 104 8 36 263 8 256 1.21e-56 187
MsG0880043307.01.T01 MTR_7g017170 47.737 243 100 9 20 250 2 229 1.58e-55 184
MsG0880043307.01.T01 MTR_7g017130 47.737 243 100 9 20 250 2 229 1.64e-55 184
MsG0880043307.01.T01 MTR_0054s0100 45.494 233 110 6 35 250 8 240 1.74e-55 184
MsG0880043307.01.T01 MTR_0129s0120 47.137 227 102 6 36 250 9 229 6.21e-55 182
MsG0880043307.01.T01 MTR_6g070880 43.210 243 112 8 30 250 18 256 1.78e-54 177
MsG0880043307.01.T01 MTR_6g071420 43.210 243 112 8 30 250 18 256 1.78e-54 177
MsG0880043307.01.T01 MTR_6g465360 47.549 204 96 4 27 219 45 248 2.54e-54 177
MsG0880043307.01.T01 MTR_6g090180 46.304 257 101 10 22 250 23 270 2.77e-54 181
MsG0880043307.01.T01 MTR_5g037680 44.398 241 97 6 42 264 6 227 5.61e-54 181
MsG0880043307.01.T01 MTR_6g086825 46.637 223 109 4 41 254 67 288 3.14e-53 179
MsG0880043307.01.T01 MTR_5g097330 42.739 241 108 7 35 253 7 239 1.15e-52 176
MsG0880043307.01.T01 MTR_2g021950 42.083 240 119 9 29 256 1 232 2.14e-52 176
MsG0880043307.01.T01 MTR_5g097350 44.493 227 96 8 53 259 25 241 2.43e-52 175
MsG0880043307.01.T01 MTR_1g076810 43.265 245 111 8 35 262 9 242 1.09e-51 179
MsG0880043307.01.T01 MTR_7g069550 43.902 246 105 9 39 263 9 242 2.08e-51 173
MsG0880043307.01.T01 MTR_6g079770 43.154 241 114 7 35 252 8 248 3.45e-50 170
MsG0880043307.01.T01 MTR_6g080680 44.628 242 106 8 35 259 10 240 7.70e-50 169
MsG0880043307.01.T01 MTR_8g013000 43.673 245 110 9 39 265 5 239 7.94e-50 169
MsG0880043307.01.T01 MTR_1g053510 42.857 238 98 6 41 247 12 242 5.34e-49 167
MsG0880043307.01.T01 MTR_8g063440 43.750 224 111 6 35 250 3 219 5.47e-49 166
MsG0880043307.01.T01 MTR_2g021770 40.486 247 113 8 34 254 2 240 8.78e-49 166
MsG0880043307.01.T01 MTR_2g038680 40.637 251 121 6 29 253 1 249 1.19e-48 166
MsG0880043307.01.T01 MTR_8g039500 43.694 222 107 8 36 253 4 211 1.30e-48 164
MsG0880043307.01.T01 MTR_4g130330 43.891 221 107 7 41 250 4 218 1.53e-48 165
MsG0880043307.01.T01 MTR_5g024260 40.909 242 117 8 41 270 19 246 4.67e-48 165
MsG0880043307.01.T01 MTR_6g461660 44.628 242 108 8 30 252 5 239 8.91e-48 164
MsG0880043307.01.T01 MTR_4g028510 44.068 236 112 8 41 259 13 245 1.61e-47 164
MsG0880043307.01.T01 MTR_2g034700 46.696 227 97 10 41 256 11 224 1.97e-47 162
MsG0880043307.01.T01 MTR_8g046310 43.578 218 92 7 42 247 14 212 2.03e-47 162
MsG0880043307.01.T01 MTR_8g073570 48.824 170 74 3 43 207 9 170 2.20e-47 155
MsG0880043307.01.T01 MTR_2g104340 42.400 250 109 9 35 262 5 241 2.21e-47 162
MsG0880043307.01.T01 MTR_1g007410 40.562 249 123 7 24 253 2 244 3.45e-47 162
MsG0880043307.01.T01 MTR_5g097680 41.732 254 114 8 29 248 1 254 3.74e-47 162
MsG0880043307.01.T01 MTR_4g028660 46.119 219 102 9 41 248 13 226 1.25e-46 160
MsG0880043307.01.T01 MTR_4g027450 46.119 219 102 9 41 248 13 226 1.25e-46 160
MsG0880043307.01.T01 MTR_5g096630 45.872 218 100 6 47 250 21 234 2.53e-46 160
MsG0880043307.01.T01 MTR_6g079810 40.075 267 130 8 19 259 44 306 3.69e-46 160
MsG0880043307.01.T01 MTR_5g097820 39.662 237 119 7 29 250 8 235 3.88e-46 155
MsG0880043307.01.T01 MTR_8g064890 44.344 221 105 7 42 250 14 228 4.75e-46 159
MsG0880043307.01.T01 MTR_4g014370 39.669 242 116 7 37 250 4 243 4.82e-46 159
MsG0880043307.01.T01 MTR_1g007450 40.476 252 122 8 36 272 4 242 1.16e-45 159
MsG0880043307.01.T01 MTR_5g072060 46.009 213 93 7 43 239 5 211 1.33e-45 162
MsG0880043307.01.T01 MTR_2g065860 42.793 222 110 6 39 250 7 221 2.13e-45 157
MsG0880043307.01.T01 MTR_5g069390 40.611 229 110 6 42 250 24 246 7.91e-45 158
MsG0880043307.01.T01 MTR_2g066170 40.693 231 117 7 36 259 4 221 1.43e-44 155
MsG0880043307.01.T01 MTR_5g059430 40.741 243 103 9 42 253 24 256 1.49e-44 155
MsG0880043307.01.T01 MTR_7g104710 41.850 227 92 6 41 259 14 208 2.49e-44 152
MsG0880043307.01.T01 MTR_5g096900 45.570 237 90 10 37 251 5 224 2.50e-44 152
MsG0880043307.01.T01 MTR_4g055820 42.083 240 109 9 39 260 9 236 2.58e-44 154
MsG0880043307.01.T01 MTR_4g028680 46.119 219 103 9 41 248 13 227 4.19e-44 154
MsG0880043307.01.T01 MTR_7g016930 43.318 217 99 7 39 250 11 208 6.87e-44 152
MsG0880043307.01.T01 MTR_7g111700 40.417 240 91 8 35 245 6 222 8.30e-44 152
MsG0880043307.01.T01 MTR_8g041270 40.400 250 114 10 39 264 10 248 8.43e-44 153
MsG0880043307.01.T01 MTR_0831s0010 39.344 244 116 8 29 255 32 260 1.23e-43 153
MsG0880043307.01.T01 MTR_2g104280 39.130 253 121 7 35 264 5 247 1.84e-43 152
MsG0880043307.01.T01 MTR_4g102410 37.354 257 132 7 10 250 34 277 2.26e-43 153
MsG0880043307.01.T01 MTR_8g464770 40.991 222 117 5 39 253 7 221 6.66e-43 150
MsG0880043307.01.T01 MTR_2g064950 42.358 229 100 8 39 250 5 218 1.27e-42 150
MsG0880043307.01.T01 MTR_2g065840 40.426 235 117 9 35 258 3 225 1.80e-42 149
MsG0880043307.01.T01 MTR_8g032980 41.833 251 95 11 35 247 5 242 2.28e-42 150
MsG0880043307.01.T01 MTR_8g046200 37.795 254 99 6 42 247 14 256 2.64e-42 150
MsG0880043307.01.T01 MTR_3g011120 41.441 222 111 8 41 253 11 222 5.00e-42 148
MsG0880043307.01.T01 MTR_8g033140 37.681 276 120 10 26 258 1 267 9.54e-42 148
MsG0880043307.01.T01 MTR_1g050308 37.657 239 129 7 20 250 10 236 1.04e-41 146
MsG0880043307.01.T01 MTR_5g037190 43.103 232 101 10 22 237 2 218 1.20e-41 144
MsG0880043307.01.T01 MTR_7g079640 41.553 219 98 7 43 253 12 208 1.99e-41 146
MsG0880043307.01.T01 MTR_8g467670 37.273 220 123 6 42 253 12 224 2.77e-41 146
MsG0880043307.01.T01 MTR_2g066150 42.135 178 96 2 36 213 4 174 5.85e-41 145
MsG0880043307.01.T01 MTR_5g074850 43.777 233 108 9 35 250 26 252 2.22e-40 149
MsG0880043307.01.T01 MTR_2g066200 39.474 228 119 6 35 253 3 220 2.76e-40 144
MsG0880043307.01.T01 MTR_8g033090 37.815 238 109 7 41 247 11 240 8.85e-40 143
MsG0880043307.01.T01 MTR_4g028750 44.037 218 105 10 41 246 13 225 9.47e-40 142
MsG0880043307.01.T01 MTR_8g033110 38.115 244 112 7 35 247 5 240 1.30e-39 142
MsG0880043307.01.T01 MTR_1g007430 43.333 210 102 7 55 252 7 211 1.34e-39 140
MsG0880043307.01.T01 MTR_5g094350 41.485 229 93 8 41 248 10 218 1.60e-39 139
MsG0880043307.01.T01 MTR_8g063330 36.638 232 128 6 41 259 11 236 3.98e-39 142
MsG0880043307.01.T01 MTR_8g064790 47.727 176 86 4 42 214 13 185 7.01e-39 139
MsG0880043307.01.T01 MTR_8g046220 36.032 247 107 6 37 247 10 241 1.69e-37 137
MsG0880043307.01.T01 MTR_8g018110 35.294 238 118 6 41 248 11 242 4.35e-37 135
MsG0880043307.01.T01 MTR_6g478230 37.657 239 124 5 19 250 2 222 8.61e-37 135
MsG0880043307.01.T01 MTR_2g104260 38.723 235 111 7 39 250 9 233 1.45e-36 131
MsG0880043307.01.T01 MTR_5g096910 39.256 242 110 10 41 259 14 241 2.01e-36 134
MsG0880043307.01.T01 MTR_5g075370 42.609 230 106 10 41 250 5 228 6.84e-36 135
MsG0880043307.01.T01 MTR_5g092850 40.090 222 113 7 43 250 15 230 2.66e-35 131
MsG0880043307.01.T01 MTR_5g091140 37.344 241 102 9 35 259 39 246 4.46e-35 130
MsG0880043307.01.T01 MTR_6g090250 41.102 236 93 12 64 259 31 260 1.97e-33 126
MsG0880043307.01.T01 MTR_8g063370 34.562 217 126 6 35 247 19 223 3.71e-33 125
MsG0880043307.01.T01 MTR_1g098180 39.320 206 93 7 41 240 16 195 9.25e-33 123
MsG0880043307.01.T01 MTR_5g097650 45.578 147 73 3 34 179 12 152 1.06e-32 117
MsG0880043307.01.T01 MTR_5g092860 40.580 207 86 8 63 251 21 208 1.18e-32 123
MsG0880043307.01.T01 MTR_1g115205 36.220 254 117 9 12 255 2 220 1.65e-31 117
MsG0880043307.01.T01 MTR_5g074800 40.609 197 91 9 83 263 1 187 8.71e-31 117
MsG0880043307.01.T01 MTR_6g078010 40.223 179 83 6 81 250 1 164 4.82e-29 113
MsG0880043307.01.T01 MTR_8g036970 38.028 213 72 7 48 250 2 164 9.18e-28 110
MsG0880043307.01.T01 MTR_3g019290 34.928 209 85 6 41 239 11 178 2.57e-27 104
MsG0880043307.01.T01 MTR_5g022850 35.841 226 86 6 39 252 11 189 8.54e-27 107
MsG0880043307.01.T01 MTR_5g096070 40.719 167 64 5 41 203 23 158 7.36e-26 100
MsG0880043307.01.T01 MTR_5g064700 39.796 196 85 8 83 248 1 193 2.78e-25 103
MsG0880043307.01.T01 MTR_4g014370 43.089 123 63 2 37 152 15 137 4.52e-25 102
MsG0880043307.01.T01 MTR_8g063310 30.328 244 141 10 32 265 10 234 3.98e-24 100
MsG0880043307.01.T01 MTR_4g007940 36.413 184 92 8 92 259 2 176 4.60e-24 99.8
MsG0880043307.01.T01 MTR_1g050312 40.278 144 63 6 112 250 16 141 2.25e-22 94.0
MsG0880043307.01.T01 MTR_8g041440 50.000 96 34 2 36 131 7 88 2.71e-22 89.4
MsG0880043307.01.T01 MTR_5g043605 29.707 239 97 6 22 255 3 175 1.30e-21 93.2
MsG0880043307.01.T01 MTR_7g023800 38.562 153 80 5 83 224 1 150 5.70e-21 90.5
MsG0880043307.01.T01 MTR_1g047080 36.413 184 93 11 81 258 1 166 3.94e-20 87.8
MsG0880043307.01.T01 MTR_5g076490 38.172 186 79 7 81 252 1 164 2.66e-19 86.3
MsG0880043307.01.T01 MTR_6g079790 37.956 137 76 3 124 254 2 135 1.01e-17 81.6
MsG0880043307.01.T01 MTR_5g049560 34.444 180 90 8 81 238 1 174 5.04e-17 79.0
MsG0880043307.01.T01 MTR_5g068490 37.037 135 62 5 121 254 8 120 8.29e-17 78.6
MsG0880043307.01.T01 MTR_8g063350 28.636 220 101 9 41 254 11 180 1.37e-16 79.0
MsG0880043307.01.T01 MTR_0667s0010 27.670 206 120 9 47 243 9 194 5.67e-16 77.4
MsG0880043307.01.T01 MTR_6g087620 34.459 148 62 4 112 254 1 118 6.47e-16 76.3
MsG0880043307.01.T01 MTR_3g013740 28.767 219 126 8 39 245 39 239 7.80e-16 77.0
MsG0880043307.01.T01 MTR_5g098950 40.909 110 56 4 161 265 90 195 1.13e-15 75.5
MsG0880043307.01.T01 MTR_4g117770 29.612 206 117 7 43 247 10 188 2.13e-15 75.5
MsG0880043307.01.T01 MTR_4g117810 29.612 206 117 7 43 247 10 188 2.13e-15 75.5
MsG0880043307.01.T01 MTR_3g047580 32.847 137 68 5 41 173 11 127 2.63e-15 71.2
MsG0880043307.01.T01 MTR_2g028130 30.137 219 110 10 47 243 8 205 5.52e-15 74.7
MsG0880043307.01.T01 MTR_2g024110 28.780 205 120 8 44 246 4 184 2.25e-14 72.8
MsG0880043307.01.T01 MTR_4g016000 41.000 100 50 3 164 259 2 96 2.43e-14 70.9
MsG0880043307.01.T01 MTR_4g116330 30.732 205 113 7 47 248 26 204 2.57e-14 72.4
MsG0880043307.01.T01 MTR_3g014570 27.536 207 120 8 47 246 32 215 2.77e-14 72.4
MsG0880043307.01.T01 MTR_4g116330 30.732 205 113 7 47 248 26 204 2.88e-14 72.4
MsG0880043307.01.T01 MTR_8g101580 30.542 203 104 7 52 246 54 227 3.09e-14 72.4
MsG0880043307.01.T01 MTR_8g033000 32.323 198 101 8 81 250 1 193 4.92e-14 71.6
MsG0880043307.01.T01 MTR_1g068835 28.708 209 120 9 42 246 2 185 6.21e-14 71.2
MsG0880043307.01.T01 MTR_2g021860 45.263 95 44 4 122 213 13 102 6.83e-14 67.8
MsG0880043307.01.T01 MTR_4g080200 27.313 227 126 10 46 263 5 201 7.89e-14 70.9
MsG0880043307.01.T01 MTR_3g006570 30.144 209 112 12 47 246 24 207 7.94e-14 71.2
MsG0880043307.01.T01 MTR_0044s0300 28.571 196 112 8 27 210 17 196 2.35e-13 69.7
MsG0880043307.01.T01 MTR_5g086780 53.226 62 27 1 29 88 1 62 3.01e-13 67.8
MsG0880043307.01.T01 MTR_3g014540 28.436 211 113 10 47 246 32 215 3.37e-13 69.3
MsG0880043307.01.T01 MTR_1g068905 30.168 179 103 8 47 223 7 165 4.29e-13 68.6
MsG0880043307.01.T01 MTR_6g090260 49.296 71 30 1 20 90 2 66 5.70e-13 67.4
MsG0880043307.01.T01 MTR_3g013770 30.409 171 93 6 47 208 36 189 1.00e-12 67.8
MsG0880043307.01.T01 MTR_2g021150 30.288 208 115 10 46 246 16 200 1.02e-12 67.8
MsG0880043307.01.T01 MTR_1g094210 26.587 252 151 8 10 256 22 244 1.23e-12 67.8
MsG0880043307.01.T01 MTR_1g094210 26.587 252 151 8 10 256 22 244 1.48e-12 67.4
MsG0880043307.01.T01 MTR_8g013010 26.667 210 126 7 43 247 53 239 2.53e-12 66.2
MsG0880043307.01.T01 MTR_4g117780 30.542 203 111 9 47 247 14 188 2.62e-12 66.6
MsG0880043307.01.T01 MTR_3g034130 29.439 214 127 10 38 247 3 196 3.41e-12 66.2
MsG0880043307.01.T01 MTR_3g103020 28.169 213 118 10 47 253 23 206 7.73e-12 65.1
MsG0880043307.01.T01 MTR_2g055970 29.646 226 121 12 29 246 195 390 1.03e-11 65.1
MsG0880043307.01.T01 MTR_1g097560 31.390 223 115 11 41 255 33 225 1.06e-11 64.7
MsG0880043307.01.T01 MTR_6g087660 47.692 65 33 1 41 104 15 79 1.70e-11 62.4
MsG0880043307.01.T01 MTR_4g117850 27.700 213 112 8 43 247 22 200 1.83e-11 63.9
MsG0880043307.01.T01 MTR_2g046900 26.733 202 129 6 46 246 30 213 1.99e-11 63.9
MsG0880043307.01.T01 MTR_7g070440 27.488 211 125 7 42 246 5 193 2.05e-11 63.9
MsG0880043307.01.T01 MTR_6g048140 27.823 248 127 11 24 250 19 235 2.55e-11 63.5
MsG0880043307.01.T01 MTR_3g034180 29.268 205 121 9 47 247 126 310 2.64e-11 63.9
MsG0880043307.01.T01 MTR_5g086590 37.500 96 43 3 159 250 13 95 2.96e-11 62.4
MsG0880043307.01.T01 MTR_3g058480 27.397 219 122 9 47 250 42 238 3.26e-11 63.2
MsG0880043307.01.T01 MTR_3g058470 26.606 218 125 8 47 250 40 236 3.86e-11 63.2
MsG0880043307.01.T01 MTR_4g133170 25.743 202 127 8 47 246 7 187 4.08e-11 63.2
MsG0880043307.01.T01 MTR_4g023090 28.431 204 119 8 47 247 19 198 4.76e-11 62.8
MsG0880043307.01.T01 MTR_2g031720 27.000 200 124 6 47 246 9 186 5.15e-11 62.8
MsG0880043307.01.T01 MTR_1g050542 28.169 213 102 8 46 246 60 233 5.99e-11 62.8
MsG0880043307.01.T01 MTR_8g467140 27.317 205 118 10 46 246 34 211 6.14e-11 62.4
MsG0880043307.01.T01 MTR_2g426060 27.315 216 121 11 47 246 9 204 6.67e-11 62.4
MsG0880043307.01.T01 MTR_2g046830 27.184 206 119 6 46 246 27 206 7.36e-11 62.4
MsG0880043307.01.T01 MTR_4g055060 28.195 266 149 14 22 269 2 243 8.28e-11 62.0
MsG0880043307.01.T01 MTR_2g028110 25.728 206 128 7 47 246 7 193 8.61e-11 62.0
MsG0880043307.01.T01 MTR_2g426020 28.889 180 101 9 47 211 9 176 8.62e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043307.01.T01 AT3G16210 25.962 208 112 8 41 245 2 170 2.08e-11 63.9

Find 56 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATACAACTTTATCAGAATT+TGG 0.172761 8:-22343379 None:intergenic
GGTGATTTGTATTCTCTTTC+CGG 0.188030 8:+22343244 MsG0880043307.01.T01:CDS
TAATTGTTTATTCCTCTAAT+TGG 0.199209 8:-22343728 None:intergenic
AAGATTAGTCTTCATGGATT+TGG 0.237806 8:+22343490 MsG0880043307.01.T01:CDS
TTTGGTTCTTGTAGTGCTAA+TGG 0.290140 8:+22343337 MsG0880043307.01.T01:CDS
CCGGTTAAGATTAGTCTTCA+TGG 0.298551 8:+22343484 MsG0880043307.01.T01:CDS
AGAATAGGACCAAACTAAAT+TGG 0.306819 8:+22343278 MsG0880043307.01.T01:CDS
CTCCTAAGTCATGGTGTAAC+AGG 0.312537 8:+22343223 MsG0880043307.01.T01:CDS
AATCGTGTCAACGATTTAAA+AGG 0.320054 8:-22343085 None:intergenic
TTGGTTCTTGTAGTGCTAAT+GGG 0.322715 8:+22343338 MsG0880043307.01.T01:CDS
AGATTAGTCTTCATGGATTT+GGG 0.331514 8:+22343491 MsG0880043307.01.T01:CDS
GTCTTTCCCTTATGATTTCC+CGG 0.345096 8:+22343465 MsG0880043307.01.T01:CDS
TAGATTTGGCCAATTTAGTT+TGG 0.348039 8:-22343287 None:intergenic
CCATGAAGACTAATCTTAAC+CGG 0.354118 8:-22343484 None:intergenic
AACGATTTAAAAGGAAGTTT+TGG 0.367916 8:-22343076 None:intergenic
GTTTGCTCTTGAGATAGATT+TGG 0.372603 8:-22343301 None:intergenic
ATGCAACGTGGACCAAAATA+AGG 0.390628 8:+22342949 MsG0880043307.01.T01:CDS
CCGGCGAGAGATTCGAGAAT+AGG 0.393214 8:+22343263 MsG0880043307.01.T01:CDS
TAGGCTATATATTTCCCATA+TGG 0.394161 8:-22343608 None:intergenic
ATCTTAACCGGGAAATCATA+AGG 0.398116 8:-22343472 None:intergenic
ACCTTGAATTCCTCGGTGGC+TGG 0.399965 8:-22343409 None:intergenic
GCCTCTTAGTCCTCTTAGTC+TGG 0.402793 8:+22343435 MsG0880043307.01.T01:CDS
AAAGGACTCATTCCAATTAG+AGG 0.404008 8:+22343716 MsG0880043307.01.T01:CDS
CGATACATGGTCATGAAACT+AGG 0.411845 8:-22343148 None:intergenic
ATTTGGGTATAACCATGTTA+TGG 0.435966 8:+22343507 MsG0880043307.01.T01:CDS
AGTACGAAGAAGAATTTGAT+TGG 0.437613 8:+22342921 MsG0880043307.01.T01:CDS
TCTTAACCGGGAAATCATAA+GGG 0.480304 8:-22343471 None:intergenic
GACGACTCAACCAGACTAAG+AGG 0.483248 8:-22343445 None:intergenic
TCCAGCCACCGAGGAATTCA+AGG 0.486712 8:+22343408 MsG0880043307.01.T01:CDS
ATAGCCTACGAAGTAACTCT+TGG 0.487141 8:+22343623 MsG0880043307.01.T01:CDS
TACGAAATTGTTGCGATACA+TGG 0.489647 8:-22343161 None:intergenic
TAATCAAATGATAATTTACA+AGG 0.495519 8:+22343693 MsG0880043307.01.T01:CDS
TGTTGATTTAAGTACTCAGA+TGG 0.520702 8:+22343657 MsG0880043307.01.T01:CDS
TCGCATGCGTGCGCAAGTCA+TGG 0.533863 8:+22343107 MsG0880043307.01.T01:CDS
ACCAGACTAAGAGGACTAAG+AGG 0.536570 8:-22343436 None:intergenic
AAATGATAATTTACAAGGAA+AGG 0.536945 8:+22343698 MsG0880043307.01.T01:CDS
GTGTCTCTCCTCCTAAGTCA+TGG 0.551520 8:+22343214 MsG0880043307.01.T01:CDS
ATTCTGATAAAGTTGTATTG+TGG 0.558638 8:+22343383 MsG0880043307.01.T01:CDS
GTGTTGTAATCTGAAGATAA+TGG 0.560617 8:-22343562 None:intergenic
CATGAAGACTAATCTTAACC+GGG 0.582470 8:-22343483 None:intergenic
CACCTGTTACACCATGACTT+AGG 0.588682 8:-22343225 None:intergenic
CTGTTACACCATGACTTAGG+AGG 0.589219 8:-22343222 None:intergenic
TAGACAAGGCAATATCATCA+TGG 0.590579 8:-22343048 None:intergenic
ATTTATAGTCATCCATAACA+TGG 0.597974 8:-22343519 None:intergenic
AGGCAATATCATCATGGACA+TGG 0.599494 8:-22343042 None:intergenic
GATACATGGTCATGAAACTA+GGG 0.602918 8:-22343147 None:intergenic
GGCAATATCATCATGGACAT+GGG 0.615477 8:-22343041 None:intergenic
AGGCACGACCTTGAATTCCT+CGG 0.619451 8:-22343416 None:intergenic
CCTATTCTCGAATCTCTCGC+CGG 0.628875 8:-22343263 None:intergenic
GTTGATTTAAGTACTCAGAT+GGG 0.633463 8:+22343658 MsG0880043307.01.T01:CDS
ATCATTTGATTATAACACTG+TGG 0.659744 8:-22343682 None:intergenic
CACGACCTTGAATTCCTCGG+TGG 0.679786 8:-22343413 None:intergenic
AATTGTTCTTCTCATCACGA+CGG 0.696708 8:+22343190 MsG0880043307.01.T01:CDS
GACTCCAAGAGTTACTTCGT+AGG 0.705260 8:-22343627 None:intergenic
TGATTGGTTAGCATGCAACG+TGG 0.715475 8:+22342937 MsG0880043307.01.T01:CDS
ATTGTGGAATCCAGCCACCG+AGG 0.751022 8:+22343399 MsG0880043307.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATCAAATGATAATTTACA+AGG + Chr8:22343693-22343712 MsG0880043307.01.T01:CDS 15.0%
!!! CAAACAAATTTTCTATTTTT+TGG + Chr8:22343319-22343338 MsG0880043307.01.T01:CDS 15.0%
!! AAATGATAATTTACAAGGAA+AGG + Chr8:22343698-22343717 MsG0880043307.01.T01:CDS 20.0%
!! AATACAACTTTATCAGAATT+TGG - Chr8:22343382-22343401 None:intergenic 20.0%
!!! TTTGTTTTTGTTCCTTATTT+TGG - Chr8:22342964-22342983 None:intergenic 20.0%
! ATCATTTGATTATAACACTG+TGG - Chr8:22343685-22343704 None:intergenic 25.0%
! ATTCTGATAAAGTTGTATTG+TGG + Chr8:22343383-22343402 MsG0880043307.01.T01:CDS 25.0%
! ATTTATAGTCATCCATAACA+TGG - Chr8:22343522-22343541 None:intergenic 25.0%
!! AACGATTTAAAAGGAAGTTT+TGG - Chr8:22343079-22343098 None:intergenic 25.0%
!!! ATTTAAAAGGAAGTTTTGGT+AGG - Chr8:22343075-22343094 None:intergenic 25.0%
AATCGTGTCAACGATTTAAA+AGG - Chr8:22343088-22343107 None:intergenic 30.0%
AGAATAGGACCAAACTAAAT+TGG + Chr8:22343278-22343297 MsG0880043307.01.T01:CDS 30.0%
AGTACGAAGAAGAATTTGAT+TGG + Chr8:22342921-22342940 MsG0880043307.01.T01:CDS 30.0%
ATTTGGGTATAACCATGTTA+TGG + Chr8:22343507-22343526 MsG0880043307.01.T01:CDS 30.0%
GTGTTGTAATCTGAAGATAA+TGG - Chr8:22343565-22343584 None:intergenic 30.0%
TAGATTTGGCCAATTTAGTT+TGG - Chr8:22343290-22343309 None:intergenic 30.0%
TAGGCTATATATTTCCCATA+TGG - Chr8:22343611-22343630 None:intergenic 30.0%
! GTTGATTTAAGTACTCAGAT+GGG + Chr8:22343658-22343677 MsG0880043307.01.T01:CDS 30.0%
! TGAGTTTTCAGAAACCATAT+GGG + Chr8:22343594-22343613 MsG0880043307.01.T01:CDS 30.0%
! TTGAGTTTTCAGAAACCATA+TGG + Chr8:22343593-22343612 MsG0880043307.01.T01:CDS 30.0%
!! AAGATTAGTCTTCATGGATT+TGG + Chr8:22343490-22343509 MsG0880043307.01.T01:CDS 30.0%
!! AGATTAGTCTTCATGGATTT+GGG + Chr8:22343491-22343510 MsG0880043307.01.T01:CDS 30.0%
!! TGTTGATTTAAGTACTCAGA+TGG + Chr8:22343657-22343676 MsG0880043307.01.T01:CDS 30.0%
AATTGTTCTTCTCATCACGA+CGG + Chr8:22343190-22343209 MsG0880043307.01.T01:CDS 35.0%
ATCTTAACCGGGAAATCATA+AGG - Chr8:22343475-22343494 None:intergenic 35.0%
CATGAAGACTAATCTTAACC+GGG - Chr8:22343486-22343505 None:intergenic 35.0%
CCATGAAGACTAATCTTAAC+CGG - Chr8:22343487-22343506 None:intergenic 35.0%
GATACATGGTCATGAAACTA+GGG - Chr8:22343150-22343169 None:intergenic 35.0%
GTTTGCTCTTGAGATAGATT+TGG - Chr8:22343304-22343323 None:intergenic 35.0%
TACGAAATTGTTGCGATACA+TGG - Chr8:22343164-22343183 None:intergenic 35.0%
TAGACAAGGCAATATCATCA+TGG - Chr8:22343051-22343070 None:intergenic 35.0%
TCTTAACCGGGAAATCATAA+GGG - Chr8:22343474-22343493 None:intergenic 35.0%
! GGTGATTTGTATTCTCTTTC+CGG + Chr8:22343244-22343263 MsG0880043307.01.T01:CDS 35.0%
! TTGGTTCTTGTAGTGCTAAT+GGG + Chr8:22343338-22343357 MsG0880043307.01.T01:CDS 35.0%
!! AAGTTTTGGTAGGATAGACA+AGG - Chr8:22343065-22343084 None:intergenic 35.0%
!! TTTGGTTCTTGTAGTGCTAA+TGG + Chr8:22343337-22343356 MsG0880043307.01.T01:CDS 35.0%
AGGCAATATCATCATGGACA+TGG - Chr8:22343045-22343064 None:intergenic 40.0%
ATAGCCTACGAAGTAACTCT+TGG + Chr8:22343623-22343642 MsG0880043307.01.T01:CDS 40.0%
ATGCAACGTGGACCAAAATA+AGG + Chr8:22342949-22342968 MsG0880043307.01.T01:CDS 40.0%
CGATACATGGTCATGAAACT+AGG - Chr8:22343151-22343170 None:intergenic 40.0%
GGCAATATCATCATGGACAT+GGG - Chr8:22343044-22343063 None:intergenic 40.0%
GTCTTTCCCTTATGATTTCC+CGG + Chr8:22343465-22343484 MsG0880043307.01.T01:CDS 40.0%
!! CCGGTTAAGATTAGTCTTCA+TGG + Chr8:22343484-22343503 MsG0880043307.01.T01:CDS 40.0%
ACCAGACTAAGAGGACTAAG+AGG - Chr8:22343439-22343458 None:intergenic 45.0%
CACCTGTTACACCATGACTT+AGG - Chr8:22343228-22343247 None:intergenic 45.0%
CTCCTAAGTCATGGTGTAAC+AGG + Chr8:22343223-22343242 MsG0880043307.01.T01:CDS 45.0%
CTGTTACACCATGACTTAGG+AGG - Chr8:22343225-22343244 None:intergenic 45.0%
GACTCCAAGAGTTACTTCGT+AGG - Chr8:22343630-22343649 None:intergenic 45.0%
! TGATTGGTTAGCATGCAACG+TGG + Chr8:22342937-22342956 MsG0880043307.01.T01:CDS 45.0%
AGGCACGACCTTGAATTCCT+CGG - Chr8:22343419-22343438 None:intergenic 50.0%
CCTATTCTCGAATCTCTCGC+CGG - Chr8:22343266-22343285 None:intergenic 50.0%
GACGACTCAACCAGACTAAG+AGG - Chr8:22343448-22343467 None:intergenic 50.0%
GCCTCTTAGTCCTCTTAGTC+TGG + Chr8:22343435-22343454 MsG0880043307.01.T01:CDS 50.0%
GTGTCTCTCCTCCTAAGTCA+TGG + Chr8:22343214-22343233 MsG0880043307.01.T01:CDS 50.0%
ACCTTGAATTCCTCGGTGGC+TGG - Chr8:22343412-22343431 None:intergenic 55.0%
ATTGTGGAATCCAGCCACCG+AGG + Chr8:22343399-22343418 MsG0880043307.01.T01:CDS 55.0%
CACGACCTTGAATTCCTCGG+TGG - Chr8:22343416-22343435 None:intergenic 55.0%
CCGGCGAGAGATTCGAGAAT+AGG + Chr8:22343263-22343282 MsG0880043307.01.T01:CDS 55.0%
TCCAGCCACCGAGGAATTCA+AGG + Chr8:22343408-22343427 MsG0880043307.01.T01:CDS 55.0%
TCGCATGCGTGCGCAAGTCA+TGG + Chr8:22343107-22343126 MsG0880043307.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 22342917 22343735 22342917 ID=MsG0880043307.01;Name=MsG0880043307.01
Chr8 mRNA 22342917 22343735 22342917 ID=MsG0880043307.01.T01;Parent=MsG0880043307.01;Name=MsG0880043307.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|272
Chr8 exon 22342917 22343735 22342917 ID=MsG0880043307.01.T01:exon:847;Parent=MsG0880043307.01.T01
Chr8 CDS 22342917 22343735 22342917 ID=MsG0880043307.01.T01:cds;Parent=MsG0880043307.01.T01
Gene Sequence

>MsG0880043307.01.T01

ATGGAGTACGAAGAAGAATTTGATTGGTTAGCATGCAACGTGGACCAAAATAAGGAACAAAAACAAAATTCTTATGTCGCTAAACTCAAACAAGAGTATGATACGTCAAGTACAAAGTTTAGCACCCATGTCCATGATGATATTGCCTTGTCTATCCTACCAAAACTTCCTTTTAAATCGTTGACACGATTCGCATGCGTGCGCAAGTCATGGTCACTCTTGTTTGAAAACCCTAGTTTCATGACCATGTATCGCAACAATTTCGTATCTAAAAATTGTTCTTCTCATCACGACGGTGTGTCTCTCCTCCTAAGTCATGGTGTAACAGGTGATTTGTATTCTCTTTCCGGCGAGAGATTCGAGAATAGGACCAAACTAAATTGGCCAAATCTATCTCAAGAGCAAACAAATTTTCTATTTTTTGGTTCTTGTAGTGCTAATGGGATTGTTTGTCTTCATGATCCAAATTCTGATAAAGTTGTATTGTGGAATCCAGCCACCGAGGAATTCAAGGTCGTGCCTCTTAGTCCTCTTAGTCTGGTTGAGTCGTCTTTCCCTTATGATTTCCCGGTTAAGATTAGTCTTCATGGATTTGGGTATAACCATGTTATGGATGACTATAAATTGATTCGCAGTGTAGCTCTTCCATTATCTTCAGATTACAACACAATGCGTGTTGAGTTTTCAGAAACCATATGGGAAATATATAGCCTACGAAGTAACTCTTGGAGTCAACTTGATGTTGATTTAAGTACTCAGATGGGACCACAGTGTTATAATCAAATGATAATTTACAAGGAAAGGACTCATTCCAATTAG
Protein sequence

>MsG0880043307.01.T01

MEYEEEFDWLACNVDQNKEQKQNSYVAKLKQEYDTSSTKFSTHVHDDIALSILPKLPFKSLTRFACVRKSWSLLFENPSFMTMYRNNFVSKNCSSHHDGVSLLLSHGVTGDLYSLSGERFENRTKLNWPNLSQEQTNFLFFGSCSANGIVCLHDPNSDKVVLWNPATEEFKVVPLSPLSLVESSFPYDFPVKISLHGFGYNHVMDDYKLIRSVALPLSSDYNTMRVEFSETIWEIYSLRSNSWSQLDVDLSTQMGPQCYNQMIIYKERTHSN*