AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043358.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043358.01.T01 MTR_8g032980 82.227 422 50 2 1 397 1 422 0.0 677
MsG0880043358.01.T01 MTR_8g033000 79.019 367 50 4 53 396 1 363 0.0 575
MsG0880043358.01.T01 MTR_8g033140 54.128 436 139 12 2 397 5 419 2.87e-141 409
MsG0880043358.01.T01 MTR_8g033090 48.712 427 173 10 1 396 1 412 2.40e-119 353
MsG0880043358.01.T01 MTR_8g033110 46.620 429 182 10 1 396 1 415 2.62e-113 337
MsG0880043358.01.T01 MTR_1g053510 48.471 425 163 11 1 392 1 402 5.79e-111 331
MsG0880043358.01.T01 MTR_8g018110 46.620 429 156 11 1 392 1 393 1.88e-110 330
MsG0880043358.01.T01 MTR_8g046200 46.067 445 164 14 1 394 1 420 1.71e-100 305
MsG0880043358.01.T01 MTR_8g046220 44.111 433 171 12 2 394 3 404 1.57e-96 294
MsG0880043358.01.T01 MTR_8g046310 47.073 410 167 15 1 394 1 376 6.24e-96 291
MsG0880043358.01.T01 MTR_0129s0070 41.766 419 198 15 1 396 1 396 9.14e-80 251
MsG0880043358.01.T01 MTR_8g063440 39.173 411 209 11 1 397 1 384 7.57e-76 240
MsG0880043358.01.T01 MTR_0129s0120 40.436 413 200 15 1 390 1 390 9.16e-75 238
MsG0880043358.01.T01 MTR_0831s0010 39.036 415 200 12 3 395 37 420 3.59e-74 237
MsG0880043358.01.T01 MTR_5g097220 39.486 428 192 13 10 396 7 408 4.39e-74 236
MsG0880043358.01.T01 MTR_3g011120 40.500 400 189 13 3 385 2 369 1.09e-73 234
MsG0880043358.01.T01 MTR_2g022000 38.626 422 196 15 2 382 4 403 5.56e-73 234
MsG0880043358.01.T01 MTR_8g464770 39.043 397 190 10 14 390 10 374 1.23e-72 232
MsG0880043358.01.T01 MTR_0129s0050 37.931 435 206 15 1 397 1 409 1.38e-72 233
MsG0880043358.01.T01 MTR_2g021800 38.710 434 194 16 8 392 2 412 3.48e-72 232
MsG0880043358.01.T01 MTR_2g066200 39.409 406 203 14 3 390 2 382 4.19e-72 231
MsG0880043358.01.T01 MTR_0054s0090 38.425 419 208 10 2 392 6 402 1.31e-71 230
MsG0880043358.01.T01 MTR_6g086825 39.423 416 220 11 2 396 56 460 5.40e-71 230
MsG0880043358.01.T01 MTR_0054s0100 37.890 417 211 11 2 390 6 402 2.02e-70 227
MsG0880043358.01.T01 MTR_5g097330 36.643 423 209 13 7 397 9 404 4.20e-70 226
MsG0880043358.01.T01 MTR_7g017130 38.741 413 216 12 2 397 6 398 1.16e-69 225
MsG0880043358.01.T01 MTR_8g064890 37.150 428 199 14 1 397 1 389 2.70e-69 223
MsG0880043358.01.T01 MTR_8g467670 36.908 401 204 10 14 397 12 380 5.36e-69 223
MsG0880043358.01.T01 MTR_8g012090 39.005 382 168 12 1 365 1 334 8.50e-69 221
MsG0880043358.01.T01 MTR_4g028680 37.385 436 196 14 2 397 3 401 1.98e-68 222
MsG0880043358.01.T01 MTR_7g017170 38.353 425 213 14 2 397 6 410 3.36e-68 221
MsG0880043358.01.T01 MTR_2g034700 40.050 402 182 16 8 389 7 369 4.37e-68 220
MsG0880043358.01.T01 MTR_4g117550 40.000 410 178 17 8 396 11 373 9.86e-68 219
MsG0880043358.01.T01 MTR_2g066170 38.329 407 213 13 4 395 2 385 2.05e-67 219
MsG0880043358.01.T01 MTR_8g039500 41.129 372 182 14 4 365 2 346 3.55e-67 217
MsG0880043358.01.T01 MTR_5g096910 37.284 405 204 10 12 393 13 390 6.33e-67 218
MsG0880043358.01.T01 MTR_4g028510 36.986 438 195 15 2 396 3 402 1.61e-66 218
MsG0880043358.01.T01 MTR_6g090180 37.232 419 210 13 6 390 33 432 2.77e-66 217
MsG0880043358.01.T01 MTR_2g038680 36.552 435 211 12 1 395 1 410 5.38e-66 216
MsG0880043358.01.T01 MTR_2g021950 35.663 415 214 14 2 390 4 391 6.66e-66 215
MsG0880043358.01.T01 MTR_4g028660 36.830 429 197 13 2 392 3 395 2.39e-65 213
MsG0880043358.01.T01 MTR_4g027450 36.830 429 197 13 2 392 3 395 2.39e-65 213
MsG0880043358.01.T01 MTR_7g079640 37.685 406 193 14 1 387 1 365 3.04e-65 213
MsG0880043358.01.T01 MTR_5g097680 36.795 443 202 16 7 396 10 427 3.34e-65 214
MsG0880043358.01.T01 MTR_8g064790 36.765 408 177 12 1 397 1 338 3.82e-65 211
MsG0880043358.01.T01 MTR_2g104340 38.837 430 195 21 1 396 1 396 5.05e-65 213
MsG0880043358.01.T01 MTR_2g021770 34.803 431 218 14 6 397 2 408 6.32e-65 213
MsG0880043358.01.T01 MTR_2g065860 36.342 421 212 13 1 397 1 389 1.88e-64 211
MsG0880043358.01.T01 MTR_5g069390 36.493 422 205 12 12 396 22 417 1.56e-63 212
MsG0880043358.01.T01 MTR_2g065840 36.451 417 214 14 1 395 1 388 1.87e-63 208
MsG0880043358.01.T01 MTR_6g079770 35.566 433 211 12 9 397 10 418 2.14e-63 209
MsG0880043358.01.T01 MTR_5g024260 37.531 405 189 14 1 371 7 381 1.03e-62 207
MsG0880043358.01.T01 MTR_4g102410 36.879 423 207 15 1 390 49 444 1.08e-62 208
MsG0880043358.01.T01 MTR_4g055820 40.945 381 174 15 1 352 1 359 1.85e-62 205
MsG0880043358.01.T01 MTR_7g069550 38.554 415 204 17 1 388 1 391 3.51e-62 205
MsG0880043358.01.T01 MTR_1g007450 37.838 407 203 16 1 382 1 382 6.06e-62 205
MsG0880043358.01.T01 MTR_6g080680 37.296 429 181 13 3 390 8 389 1.79e-61 203
MsG0880043358.01.T01 MTR_5g037680 36.253 411 198 14 12 391 4 381 3.54e-61 204
MsG0880043358.01.T01 MTR_2g064950 35.610 410 201 15 8 387 4 380 4.38e-61 202
MsG0880043358.01.T01 MTR_5g059430 36.468 436 212 19 1 392 8 422 4.96e-61 203
MsG0880043358.01.T01 MTR_4g014370 35.280 428 217 15 8 397 5 410 5.65e-61 202
MsG0880043358.01.T01 MTR_4g028750 36.186 409 187 12 1 371 1 373 2.56e-59 198
MsG0880043358.01.T01 MTR_6g079810 34.873 433 207 14 7 393 57 460 2.94e-59 199
MsG0880043358.01.T01 MTR_7g017040 35.476 420 225 11 1 396 1 398 3.29e-59 197
MsG0880043358.01.T01 MTR_4g007940 40.179 336 161 9 80 396 28 342 3.74e-59 196
MsG0880043358.01.T01 MTR_8g036970 36.533 375 152 10 20 378 2 306 9.88e-59 196
MsG0880043358.01.T01 MTR_4g130330 35.437 412 220 12 10 396 1 391 1.26e-58 196
MsG0880043358.01.T01 MTR_5g096630 36.175 434 215 16 1 397 7 415 1.65e-58 196
MsG0880043358.01.T01 MTR_1g007410 37.469 403 198 14 15 388 21 398 1.65e-58 196
MsG0880043358.01.T01 MTR_6g478230 35.407 418 208 12 11 397 22 408 3.02e-58 196
MsG0880043358.01.T01 MTR_8g041270 35.797 433 214 18 1 396 1 406 3.63e-58 195
MsG0880043358.01.T01 MTR_0134s0060 36.322 435 210 16 3 395 2 411 9.60e-58 194
MsG0880043358.01.T01 MTR_8g063330 37.566 378 175 13 4 351 2 348 5.22e-57 194
MsG0880043358.01.T01 MTR_5g097350 36.364 407 176 15 25 394 25 385 1.25e-56 191
MsG0880043358.01.T01 MTR_8g013000 36.339 366 194 10 15 358 9 357 1.77e-56 191
MsG0880043358.01.T01 MTR_2g104280 37.470 419 211 18 9 396 7 405 1.47e-55 188
MsG0880043358.01.T01 MTR_6g461660 36.318 402 219 13 14 396 17 400 6.09e-55 186
MsG0880043358.01.T01 MTR_8g035690 41.786 280 120 7 144 395 38 302 2.33e-54 182
MsG0880043358.01.T01 MTR_5g092850 33.958 427 222 11 1 394 1 400 2.11e-53 182
MsG0880043358.01.T01 MTR_7g111700 33.255 427 167 12 15 397 14 366 1.53e-52 179
MsG0880043358.01.T01 MTR_5g072060 35.572 402 190 15 15 382 5 371 4.56e-51 181
MsG0880043358.01.T01 MTR_5g074850 34.856 416 202 16 1 378 22 406 1.72e-50 182
MsG0880043358.01.T01 MTR_1g047080 34.771 371 127 10 53 396 1 283 3.50e-50 171
MsG0880043358.01.T01 MTR_1g098180 33.017 421 196 12 1 393 1 363 5.63e-50 172
MsG0880043358.01.T01 MTR_2g066150 35.135 407 204 14 4 397 2 361 6.68e-50 172
MsG0880043358.01.T01 MTR_5g091140 33.747 403 185 13 2 370 36 390 3.71e-47 166
MsG0880043358.01.T01 MTR_6g078010 34.916 358 184 13 53 390 1 329 2.71e-46 162
MsG0880043358.01.T01 MTR_5g075370 32.524 412 198 14 11 376 3 380 5.64e-46 169
MsG0880043358.01.T01 MTR_5g022850 31.981 419 198 18 1 397 1 354 9.30e-45 158
MsG0880043358.01.T01 MTR_4g014370 30.556 396 196 10 8 397 16 338 2.42e-44 157
MsG0880043358.01.T01 MTR_1g007430 36.937 333 170 13 27 344 7 314 2.62e-44 156
MsG0880043358.01.T01 MTR_6g090250 33.416 404 208 12 36 392 31 420 3.80e-43 155
MsG0880043358.01.T01 MTR_8g063370 29.975 407 240 13 1 387 1 382 1.13e-42 154
MsG0880043358.01.T01 MTR_5g043605 34.321 405 156 18 14 396 23 339 1.25e-42 153
MsG0880043358.01.T01 MTR_5g096900 35.312 337 166 11 8 319 4 313 3.33e-41 148
MsG0880043358.01.T01 MTR_5g074800 35.342 365 176 17 55 388 1 336 3.63e-41 149
MsG0880043358.01.T01 MTR_7g016930 35.604 323 177 10 8 327 10 304 4.60e-41 148
MsG0880043358.01.T01 MTR_5g064700 31.980 394 190 13 55 396 1 368 4.10e-40 146
MsG0880043358.01.T01 MTR_8g063310 28.788 396 233 12 14 387 20 388 2.25e-39 145
MsG0880043358.01.T01 MTR_5g092860 32.440 373 177 13 35 374 21 351 5.71e-39 143
MsG0880043358.01.T01 MTR_7g104710 36.111 324 132 14 2 302 6 277 1.12e-38 141
MsG0880043358.01.T01 MTR_5g097820 36.981 265 126 9 1 242 8 254 6.85e-38 138
MsG0880043358.01.T01 MTR_5g086590 36.782 261 108 7 128 368 8 231 6.86e-38 137
MsG0880043358.01.T01 MTR_5g068490 34.688 320 149 12 89 397 8 278 8.74e-38 138
MsG0880043358.01.T01 MTR_7g023800 33.143 350 169 13 55 387 1 302 1.06e-37 138
MsG0880043358.01.T01 MTR_8g063350 29.602 402 198 12 11 394 9 343 2.07e-37 139
MsG0880043358.01.T01 MTR_5g097850 36.330 267 99 7 115 371 16 221 7.45e-36 132
MsG0880043358.01.T01 MTR_6g070880 39.357 249 120 10 3 228 21 261 2.07e-35 131
MsG0880043358.01.T01 MTR_6g071420 39.357 249 120 10 3 228 21 261 2.07e-35 131
MsG0880043358.01.T01 MTR_6g090260 42.424 198 91 6 206 392 75 260 1.07e-34 129
MsG0880043358.01.T01 MTR_6g090260 62.687 67 23 2 1 67 7 71 5.60e-16 77.8
MsG0880043358.01.T01 MTR_6g079790 33.876 307 160 8 116 397 22 310 1.08e-33 128
MsG0880043358.01.T01 MTR_1g050308 36.515 241 121 8 1 230 23 242 1.09e-32 126
MsG0880043358.01.T01 MTR_1g050308 41.739 115 62 2 285 397 237 348 6.56e-17 81.6
MsG0880043358.01.T01 MTR_8g073570 48.630 146 61 6 10 148 4 142 5.19e-32 119
MsG0880043358.01.T01 MTR_1g076810 35.510 245 112 7 2 224 6 226 4.03e-31 125
MsG0880043358.01.T01 MTR_1g050312 31.902 326 171 12 65 385 5 284 5.41e-31 120
MsG0880043358.01.T01 MTR_5g094350 32.423 293 126 9 14 265 11 272 1.14e-30 119
MsG0880043358.01.T01 MTR_5g023970 37.931 203 105 5 203 397 8 197 1.21e-30 116
MsG0880043358.01.T01 MTR_5g097650 42.138 159 83 4 1 159 7 156 4.60e-30 114
MsG0880043358.01.T01 MTR_5g037190 32.872 289 133 11 2 270 4 251 1.01e-29 115
MsG0880043358.01.T01 MTR_2g021920 37.931 203 97 7 201 390 81 267 6.03e-29 114
MsG0880043358.01.T01 MTR_6g087620 31.288 326 158 12 80 386 1 279 1.33e-28 113
MsG0880043358.01.T01 MTR_6g465360 46.061 165 61 8 4 150 45 199 3.19e-28 112
MsG0880043358.01.T01 MTR_5g076490 31.937 382 176 15 53 396 1 336 5.37e-28 113
MsG0880043358.01.T01 MTR_3g018750 36.620 213 96 5 205 396 47 241 2.63e-27 108
MsG0880043358.01.T01 MTR_7g019250 40.642 187 88 6 186 371 247 411 3.09e-27 113
MsG0880043358.01.T01 MTR_6g004450 40.206 194 89 4 206 390 88 263 1.34e-26 107
MsG0880043358.01.T01 MTR_4g016000 37.557 221 100 8 138 349 2 193 2.63e-26 105
MsG0880043358.01.T01 MTR_3g019290 37.799 209 68 6 4 184 2 176 3.66e-26 103
MsG0880043358.01.T01 MTR_1g115205 33.068 251 128 8 2 241 14 235 1.43e-24 101
MsG0880043358.01.T01 MTR_3g047580 39.310 145 69 4 3 147 2 127 2.37e-24 97.4
MsG0880043358.01.T01 MTR_6g009350 39.674 184 79 7 188 359 32 195 5.33e-24 98.6
MsG0880043358.01.T01 MTR_6g079730 43.609 133 68 2 265 392 16 146 2.38e-23 95.5
MsG0880043358.01.T01 MTR_5g066420 39.706 136 71 1 250 385 25 149 8.00e-23 94.0
MsG0880043358.01.T01 MTR_5g096920 36.196 163 86 4 235 393 1 149 3.12e-22 92.4
MsG0880043358.01.T01 MTR_3g030620 37.870 169 89 6 232 393 20 179 5.12e-22 92.8
MsG0880043358.01.T01 MTR_5g096070 43.478 138 53 5 14 150 24 137 1.38e-21 90.9
MsG0880043358.01.T01 MTR_5g097660 45.133 113 61 1 283 394 6 118 1.39e-20 87.0
MsG0880043358.01.T01 MTR_5g098950 34.222 225 103 8 135 354 90 274 1.61e-20 90.9
MsG0880043358.01.T01 MTR_7g017150 39.196 199 97 7 205 393 81 265 2.45e-20 90.1
MsG0880043358.01.T01 MTR_7g017120 39.196 199 97 7 205 393 80 264 3.46e-20 89.7
MsG0880043358.01.T01 MTR_5g036250 51.136 88 42 1 309 396 25 111 1.03e-19 86.7
MsG0880043358.01.T01 MTR_2g104260 33.447 293 142 13 1 258 1 275 2.13e-19 87.8
MsG0880043358.01.T01 MTR_2g022020 39.091 110 65 1 282 391 8 115 1.28e-18 81.3
MsG0880043358.01.T01 MTR_5g097540 37.908 153 77 4 205 354 96 233 1.86e-18 85.5
MsG0880043358.01.T01 MTR_8g041440 50.000 88 34 1 10 97 9 86 9.60e-18 79.0
MsG0880043358.01.T01 MTR_8g090195 64.407 59 21 0 314 372 57 115 2.13e-17 78.2
MsG0880043358.01.T01 MTR_5g086780 39.850 133 77 3 240 371 97 227 3.55e-17 80.5
MsG0880043358.01.T01 MTR_5g086780 58.065 62 24 1 1 60 1 62 7.15e-14 70.9
MsG0880043358.01.T01 MTR_0064s0160 24.038 416 255 16 10 394 36 421 9.69e-17 82.0
MsG0880043358.01.T01 MTR_1g061330 23.308 399 240 13 19 396 7 360 2.39e-16 80.5
MsG0880043358.01.T01 MTR_0115s0020 26.415 371 198 16 10 343 4 336 1.63e-15 78.2
MsG0880043358.01.T01 MTR_3g030350 22.753 356 207 12 19 359 38 340 3.46e-14 73.9
MsG0880043358.01.T01 MTR_7g101200 30.973 226 114 11 134 353 39 228 4.91e-14 71.6
MsG0880043358.01.T01 MTR_0044s0300 22.750 400 246 14 1 382 14 368 6.41e-14 73.2
MsG0880043358.01.T01 MTR_3g006530 23.476 328 194 12 19 331 43 328 7.87e-14 72.8
MsG0880043358.01.T01 MTR_4g116330 25.123 406 238 17 7 392 14 373 1.42e-13 72.0
MsG0880043358.01.T01 MTR_1g442850 26.946 334 197 18 14 331 3 305 1.70e-13 71.6
MsG0880043358.01.T01 MTR_5g091150 30.412 194 83 7 115 305 43 187 3.20e-13 68.6
MsG0880043358.01.T01 MTR_2g021150 25.373 335 191 15 18 335 16 308 3.76e-13 70.5
MsG0880043358.01.T01 MTR_2g031720 26.250 320 200 13 19 331 9 299 3.95e-13 70.9
MsG0880043358.01.T01 MTR_5g096050 46.591 88 33 2 248 334 30 104 6.37e-13 65.5
MsG0880043358.01.T01 MTR_7g060960 25.152 330 182 13 11 332 27 299 6.68e-13 69.7
MsG0880043358.01.T01 MTR_1g050305 49.367 79 35 3 297 370 21 99 2.63e-12 64.3
MsG0880043358.01.T01 MTR_5g048160 24.645 422 234 21 19 394 7 390 3.19e-11 64.7
MsG0880043358.01.T01 MTR_1g054725 22.841 359 218 13 14 358 18 331 4.71e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 88 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGAGGAAGTTTGGCTTAA+TGG 0.159889 8:+23602631 MsG0880043358.01.T01:CDS
TTGTATTCACATATGGGTTT+TGG 0.187456 8:+23602456 MsG0880043358.01.T01:CDS
CTGTATATCTCCCAATAATT+AGG 0.191262 8:-23602136 None:intergenic
ACAAAATTGGTGGTATTAAA+TGG 0.194059 8:+23602397 MsG0880043358.01.T01:CDS
TTAGGGTCATACATATTTAT+TGG 0.223413 8:-23602118 None:intergenic
GAACACCAATTGCGAATAAA+TGG 0.254719 8:-23601785 None:intergenic
GTCTTCTTCCTCATTATGTT+TGG 0.260793 8:-23601648 None:intergenic
GAGGGATTTAAGAGGCAATT+TGG 0.261100 8:-23601540 None:intergenic
CAGAGAAGAATAGCCCTTTC+TGG 0.264414 8:+23602047 MsG0880043358.01.T01:CDS
TGGTGTTCAGCTAGTGTTAA+TGG 0.277178 8:+23601800 MsG0880043358.01.T01:CDS
TGTACAAAGCGTCATGATTT+AGG 0.280398 8:-23601685 None:intergenic
TGACTTCTTGTATTCACATA+TGG 0.286028 8:+23602449 MsG0880043358.01.T01:CDS
ATATGTATGACCCTAATTAT+TGG 0.291690 8:+23602125 MsG0880043358.01.T01:CDS
CCGAAAGGATTGAGGAAGTT+TGG 0.295469 8:+23602623 MsG0880043358.01.T01:CDS
CGATCATAACCAAATGAAAA+AGG 0.300970 8:-23601950 None:intergenic
ACGATGATGTTATGCATTCT+TGG 0.318927 8:+23602188 MsG0880043358.01.T01:CDS
TATGTATGACCCTAATTATT+GGG 0.362027 8:+23602126 MsG0880043358.01.T01:CDS
GTTGGGCCTAGATAATGATA+TGG 0.363205 8:+23602252 MsG0880043358.01.T01:CDS
CAGGAAGTTGGAGACAAAAT+TGG 0.373652 8:+23602384 MsG0880043358.01.T01:CDS
GTCTTAAATTCCCTGGTGGC+TGG 0.386234 8:-23601878 None:intergenic
GTTGTCCATTTATTCGCAAT+TGG 0.395813 8:+23601780 MsG0880043358.01.T01:CDS
GACTTCTTGTATTCACATAT+GGG 0.397176 8:+23602450 MsG0880043358.01.T01:CDS
TGTATATCTCCCAATAATTA+GGG 0.397454 8:-23602135 None:intergenic
GATAGAGGATACTCCCTCTA+AGG 0.398703 8:-23602347 None:intergenic
TAGTAAAGAGACATCCTTAG+AGG 0.399313 8:+23602333 MsG0880043358.01.T01:CDS
AGTACTAACCTTCTTCTTAT+CGG 0.399380 8:-23601477 None:intergenic
CCAAACTTCCTCAATCCTTT+CGG 0.434132 8:-23602623 None:intergenic
ACGACCCCATATCATTATCT+AGG 0.437117 8:-23602258 None:intergenic
GTTATAATTTGCAACCTTCG+TGG 0.456109 8:-23602658 None:intergenic
TTTGTTGTTGGACCTTTACC+TGG 0.456180 8:+23602514 MsG0880043358.01.T01:CDS
GGCATTCTTTGTCTCTCTCA+AGG 0.460806 8:+23601821 MsG0880043358.01.T01:CDS
AGAGAAGAATAGCCCTTTCT+GGG 0.461754 8:+23602048 MsG0880043358.01.T01:CDS
CAGAAAGGGCTATTCTTCTC+TGG 0.462996 8:-23602046 None:intergenic
CGATGATGTTATGCATTCTT+GGG 0.466567 8:+23602189 MsG0880043358.01.T01:CDS
TGATTTCAATCGCAGGAAGT+TGG 0.468967 8:+23602372 MsG0880043358.01.T01:CDS
TTGGGCCTAGATAATGATAT+GGG 0.471212 8:+23602253 MsG0880043358.01.T01:CDS
TTCTTGGGATGAGCGTTGTC+AGG 0.479205 8:+23602204 MsG0880043358.01.T01:CDS
GAATAGCCCTTTCTGGGAGA+TGG 0.483505 8:+23602054 MsG0880043358.01.T01:CDS
CGAAACATGTTCATGAAATG+AGG 0.484377 8:-23601611 None:intergenic
GCGATTGAAATCATAGATAG+AGG 0.490135 8:-23602362 None:intergenic
TCATTCTTCCAAAAGAAAGA+AGG 0.493128 8:-23602693 None:intergenic
AATCTTGTGAGGGATTTAAG+AGG 0.495597 8:-23601548 None:intergenic
ACAGCCTCAGAAGTAACTCT+TGG 0.497294 8:+23602155 MsG0880043358.01.T01:CDS
GCCTCAGAAGTAACTCTTGG+AGG 0.497466 8:+23602158 MsG0880043358.01.T01:CDS
TCTAAGTCCATCTCCCAGAA+AGG 0.503391 8:-23602061 None:intergenic
TCACTTGTGCTAAGAAATCA+TGG 0.504012 8:+23601570 MsG0880043358.01.T01:CDS
CTTCTACATAAAAGAAGATA+CGG 0.512467 8:+23602576 MsG0880043358.01.T01:CDS
TATTGTGGAATCCAGCCACC+AGG 0.527866 8:+23601867 MsG0880043358.01.T01:CDS
CTTATCGGTTGCAGCAGCCA+TGG 0.528350 8:-23601462 None:intergenic
CTAAGTCCATCTCCCAGAAA+GGG 0.529945 8:-23602060 None:intergenic
TTTCATATCCAAACATAATG+AGG 0.530352 8:+23601640 MsG0880043358.01.T01:CDS
TTTAAGTACCGAAAGGATTG+AGG 0.538703 8:+23602615 MsG0880043358.01.T01:CDS
CTCTTTATTCCTACTGCGAT+AGG 0.544707 8:-23602547 None:intergenic
TCGTATGGTCGATGGCTACA+AGG 0.544850 8:-23601905 None:intergenic
ATTCCACAATACAATGGTCA+TGG 0.555152 8:-23601855 None:intergenic
TCTATTCTCAAAACTCTCAC+CGG 0.559574 8:-23601714 None:intergenic
CGTTGAAAATGAAGAGGAGG+TGG 0.562134 8:-23601752 None:intergenic
TTAAACTCAGTGTTCTCGTA+TGG 0.568341 8:-23601920 None:intergenic
AGTAAAGAGACATCCTTAGA+GGG 0.575472 8:+23602334 MsG0880043358.01.T01:CDS
AGGAACGGTCTTAAATTCCC+TGG 0.576392 8:-23601885 None:intergenic
TGCTGCAACCGATAAGAAGA+AGG 0.577624 8:+23601469 MsG0880043358.01.T01:CDS
CAGTGTTCTCGTATGGTCGA+TGG 0.577759 8:-23601913 None:intergenic
CTATCTATGATTTCAATCGC+AGG 0.578132 8:+23602365 MsG0880043358.01.T01:CDS
GGACGGATAATACCAGGTAA+AGG 0.578490 8:-23602526 None:intergenic
TCTTTACTAAAGTCAAATGA+AGG 0.579020 8:-23602319 None:intergenic
TTCCACAATACAATGGTCAT+GGG 0.589187 8:-23601854 None:intergenic
CCCTCTCGTTGAAAATGAAG+AGG 0.591712 8:-23601758 None:intergenic
AGCACAAGTGAATCTTGTGA+GGG 0.591745 8:-23601558 None:intergenic
ATTATCCGTCCTATCGCAGT+AGG 0.596125 8:+23602538 MsG0880043358.01.T01:CDS
TAGCACAAGTGAATCTTGTG+AGG 0.598088 8:-23601559 None:intergenic
ATCCCATGACCATTGTATTG+TGG 0.598550 8:+23601852 MsG0880043358.01.T01:CDS
TGGTCGATGGCTACAAGGAA+CGG 0.598781 8:-23601900 None:intergenic
AACTTGGTGTTAAAGAATCG+TGG 0.605182 8:+23602482 MsG0880043358.01.T01:CDS
GAAACATGTTCATGAAATGA+GGG 0.606512 8:-23601610 None:intergenic
GAAGTTGGAGACAAAATTGG+TGG 0.606616 8:+23602387 MsG0880043358.01.T01:CDS
TTATTCCTACTGCGATAGGA+CGG 0.613701 8:-23602543 None:intergenic
GGCTTAATGGAGAGCCACGA+AGG 0.614050 8:+23602644 MsG0880043358.01.T01:CDS
TCCTCCAAGAGTTACTTCTG+AGG 0.626964 8:-23602159 None:intergenic
ATGTCATCGTGTATATGCGA+GGG 0.631015 8:-23601500 None:intergenic
GCGATAGGACGGATAATACC+AGG 0.642333 8:-23602532 None:intergenic
CATTATCTAGGCCCAACCAA+TGG 0.642611 8:-23602246 None:intergenic
TCTCGTTGAAAATGAAGAGG+AGG 0.658054 8:-23601755 None:intergenic
TGTGAATACAAGAAGTCATG+AGG 0.659013 8:-23602444 None:intergenic
AACGGTCTTAAATTCCCTGG+TGG 0.665952 8:-23601882 None:intergenic
AATGTCATCGTGTATATGCG+AGG 0.666213 8:-23601501 None:intergenic
GGCTGGATTCCACAATACAA+TGG 0.669031 8:-23601861 None:intergenic
ATTGTGGAATCCAGCCACCA+GGG 0.683215 8:+23601868 MsG0880043358.01.T01:CDS
TGGGCCTAGATAATGATATG+GGG 0.710319 8:+23602254 MsG0880043358.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGATGATAATGATGATTTTT+GGG + Chr8:23602087-23602106 MsG0880043358.01.T01:CDS 20.0%
!!! TTGATGATAATGATGATTTT+TGG + Chr8:23602086-23602105 MsG0880043358.01.T01:CDS 20.0%
! ATATGTATGACCCTAATTAT+TGG + Chr8:23602125-23602144 MsG0880043358.01.T01:CDS 25.0%
! CTTCTACATAAAAGAAGATA+CGG + Chr8:23602576-23602595 MsG0880043358.01.T01:CDS 25.0%
! TATGTATGACCCTAATTATT+GGG + Chr8:23602126-23602145 MsG0880043358.01.T01:CDS 25.0%
! TCTTTACTAAAGTCAAATGA+AGG - Chr8:23602322-23602341 None:intergenic 25.0%
! TGTATATCTCCCAATAATTA+GGG - Chr8:23602138-23602157 None:intergenic 25.0%
! TTAGGGTCATACATATTTAT+TGG - Chr8:23602121-23602140 None:intergenic 25.0%
! TTTCATATCCAAACATAATG+AGG + Chr8:23601640-23601659 MsG0880043358.01.T01:CDS 25.0%
!! AAGAAAAACCTTCTTTCTTT+TGG + Chr8:23602685-23602704 MsG0880043358.01.T01:CDS 25.0%
!! ACAAAATTGGTGGTATTAAA+TGG + Chr8:23602397-23602416 MsG0880043358.01.T01:CDS 25.0%
!! ATTTGAATGAATTTTGCCAT+TGG + Chr8:23602230-23602249 MsG0880043358.01.T01:CDS 25.0%
!!! AATACTCAACCTTTTTCATT+TGG + Chr8:23601941-23601960 MsG0880043358.01.T01:CDS 25.0%
!!! AATTGTCACATATTTTTCGA+AGG + Chr8:23602007-23602026 MsG0880043358.01.T01:intron 25.0%
AGTACTAACCTTCTTCTTAT+CGG - Chr8:23601480-23601499 None:intergenic 30.0%
ATTTGGTTATGATCGTGTTA+GGG + Chr8:23601958-23601977 MsG0880043358.01.T01:intron 30.0%
CGATCATAACCAAATGAAAA+AGG - Chr8:23601953-23601972 None:intergenic 30.0%
CTGTATATCTCCCAATAATT+AGG - Chr8:23602139-23602158 None:intergenic 30.0%
GAAACATGTTCATGAAATGA+GGG - Chr8:23601613-23601632 None:intergenic 30.0%
GACTTCTTGTATTCACATAT+GGG + Chr8:23602450-23602469 MsG0880043358.01.T01:CDS 30.0%
TCATTCTTCCAAAAGAAAGA+AGG - Chr8:23602696-23602715 None:intergenic 30.0%
TGACTTCTTGTATTCACATA+TGG + Chr8:23602449-23602468 MsG0880043358.01.T01:CDS 30.0%
! TGTATTCACATATGGGTTTT+GGG + Chr8:23602457-23602476 MsG0880043358.01.T01:CDS 30.0%
! TTGTATTCACATATGGGTTT+TGG + Chr8:23602456-23602475 MsG0880043358.01.T01:CDS 30.0%
!! AATGAATTTTGCCATTGGTT+GGG + Chr8:23602235-23602254 MsG0880043358.01.T01:CDS 30.0%
!! GATAATGATGATTTTTGGGA+GGG + Chr8:23602091-23602110 MsG0880043358.01.T01:CDS 30.0%
!! TGATAATGATGATTTTTGGG+AGG + Chr8:23602090-23602109 MsG0880043358.01.T01:CDS 30.0%
!! TGGACAAAACTTTTTGTTGT+TGG + Chr8:23602502-23602521 MsG0880043358.01.T01:CDS 30.0%
!!! GTTTTGATTTAAGTACCGAA+AGG + Chr8:23602608-23602627 MsG0880043358.01.T01:CDS 30.0%
AATCTTGTGAGGGATTTAAG+AGG - Chr8:23601551-23601570 None:intergenic 35.0%
ACGATGATGTTATGCATTCT+TGG + Chr8:23602188-23602207 MsG0880043358.01.T01:CDS 35.0%
AGTAAAGAGACATCCTTAGA+GGG + Chr8:23602334-23602353 MsG0880043358.01.T01:CDS 35.0%
ATTCCACAATACAATGGTCA+TGG - Chr8:23601858-23601877 None:intergenic 35.0%
CATTTGGTTATGATCGTGTT+AGG + Chr8:23601957-23601976 MsG0880043358.01.T01:intron 35.0%
CGAAACATGTTCATGAAATG+AGG - Chr8:23601614-23601633 None:intergenic 35.0%
CGATGATGTTATGCATTCTT+GGG + Chr8:23602189-23602208 MsG0880043358.01.T01:CDS 35.0%
CTATCTATGATTTCAATCGC+AGG + Chr8:23602365-23602384 MsG0880043358.01.T01:CDS 35.0%
GAACACCAATTGCGAATAAA+TGG - Chr8:23601788-23601807 None:intergenic 35.0%
GCGATTGAAATCATAGATAG+AGG - Chr8:23602365-23602384 None:intergenic 35.0%
GTCTTCTTCCTCATTATGTT+TGG - Chr8:23601651-23601670 None:intergenic 35.0%
GTTGTCCATTTATTCGCAAT+TGG + Chr8:23601780-23601799 MsG0880043358.01.T01:CDS 35.0%
TAGTAAAGAGACATCCTTAG+AGG + Chr8:23602333-23602352 MsG0880043358.01.T01:CDS 35.0%
TCACTTGTGCTAAGAAATCA+TGG + Chr8:23601570-23601589 MsG0880043358.01.T01:CDS 35.0%
TCTATTCTCAAAACTCTCAC+CGG - Chr8:23601717-23601736 None:intergenic 35.0%
TGTGAATACAAGAAGTCATG+AGG - Chr8:23602447-23602466 None:intergenic 35.0%
TTAAACTCAGTGTTCTCGTA+TGG - Chr8:23601923-23601942 None:intergenic 35.0%
TTCCACAATACAATGGTCAT+GGG - Chr8:23601857-23601876 None:intergenic 35.0%
TTGGGCCTAGATAATGATAT+GGG + Chr8:23602253-23602272 MsG0880043358.01.T01:CDS 35.0%
! ATTGAGGAAGTTTGGCTTAA+TGG + Chr8:23602631-23602650 MsG0880043358.01.T01:CDS 35.0%
! GAATGAATTTTGCCATTGGT+TGG + Chr8:23602234-23602253 MsG0880043358.01.T01:CDS 35.0%
! GTTATAATTTGCAACCTTCG+TGG - Chr8:23602661-23602680 None:intergenic 35.0%
! TGTACAAAGCGTCATGATTT+AGG - Chr8:23601688-23601707 None:intergenic 35.0%
!! AACTTGGTGTTAAAGAATCG+TGG + Chr8:23602482-23602501 MsG0880043358.01.T01:CDS 35.0%
!! TTTAAGTACCGAAAGGATTG+AGG + Chr8:23602615-23602634 MsG0880043358.01.T01:CDS 35.0%
!!! ACATATTTTTCGAAGGTGCT+TGG + Chr8:23602014-23602033 MsG0880043358.01.T01:intron 35.0%
!!! CATATTTTTCGAAGGTGCTT+GGG + Chr8:23602015-23602034 MsG0880043358.01.T01:intron 35.0%
AATGTCATCGTGTATATGCG+AGG - Chr8:23601504-23601523 None:intergenic 40.0%
ACGACCCCATATCATTATCT+AGG - Chr8:23602261-23602280 None:intergenic 40.0%
AGAGAAGAATAGCCCTTTCT+GGG + Chr8:23602048-23602067 MsG0880043358.01.T01:CDS 40.0%
AGCACAAGTGAATCTTGTGA+GGG - Chr8:23601561-23601580 None:intergenic 40.0%
ATCCCATGACCATTGTATTG+TGG + Chr8:23601852-23601871 MsG0880043358.01.T01:CDS 40.0%
ATGTCATCGTGTATATGCGA+GGG - Chr8:23601503-23601522 None:intergenic 40.0%
CAGGAAGTTGGAGACAAAAT+TGG + Chr8:23602384-23602403 MsG0880043358.01.T01:CDS 40.0%
CCAAACTTCCTCAATCCTTT+CGG - Chr8:23602626-23602645 None:intergenic 40.0%
CTCTTTATTCCTACTGCGAT+AGG - Chr8:23602550-23602569 None:intergenic 40.0%
GAAGTTGGAGACAAAATTGG+TGG + Chr8:23602387-23602406 MsG0880043358.01.T01:CDS 40.0%
GAGGGATTTAAGAGGCAATT+TGG - Chr8:23601543-23601562 None:intergenic 40.0%
GTTGGGCCTAGATAATGATA+TGG + Chr8:23602252-23602271 MsG0880043358.01.T01:CDS 40.0%
TAGCACAAGTGAATCTTGTG+AGG - Chr8:23601562-23601581 None:intergenic 40.0%
TCTCGTTGAAAATGAAGAGG+AGG - Chr8:23601758-23601777 None:intergenic 40.0%
TGATTTCAATCGCAGGAAGT+TGG + Chr8:23602372-23602391 MsG0880043358.01.T01:CDS 40.0%
TGGGCCTAGATAATGATATG+GGG + Chr8:23602254-23602273 MsG0880043358.01.T01:CDS 40.0%
TGGTGTTCAGCTAGTGTTAA+TGG + Chr8:23601800-23601819 MsG0880043358.01.T01:CDS 40.0%
TTATTCCTACTGCGATAGGA+CGG - Chr8:23602546-23602565 None:intergenic 40.0%
! CCTCTTCATTTTCAACGAGA+GGG + Chr8:23601758-23601777 MsG0880043358.01.T01:CDS 40.0%
! GACGCTTTGTACACATTTTC+CGG + Chr8:23601695-23601714 MsG0880043358.01.T01:CDS 40.0%
! TCCTCTTCATTTTCAACGAG+AGG + Chr8:23601757-23601776 MsG0880043358.01.T01:CDS 40.0%
!! TTTGTTGTTGGACCTTTACC+TGG + Chr8:23602514-23602533 MsG0880043358.01.T01:CDS 40.0%
!!! ATATGGGTTTTGGGTGAACT+TGG + Chr8:23602466-23602485 MsG0880043358.01.T01:CDS 40.0%
AACGGTCTTAAATTCCCTGG+TGG - Chr8:23601885-23601904 None:intergenic 45.0%
ACAGCCTCAGAAGTAACTCT+TGG + Chr8:23602155-23602174 MsG0880043358.01.T01:CDS 45.0%
AGGAACGGTCTTAAATTCCC+TGG - Chr8:23601888-23601907 None:intergenic 45.0%
ATTATCCGTCCTATCGCAGT+AGG + Chr8:23602538-23602557 MsG0880043358.01.T01:CDS 45.0%
CAGAAAGGGCTATTCTTCTC+TGG - Chr8:23602049-23602068 None:intergenic 45.0%
CAGAGAAGAATAGCCCTTTC+TGG + Chr8:23602047-23602066 MsG0880043358.01.T01:CDS 45.0%
CATTATCTAGGCCCAACCAA+TGG - Chr8:23602249-23602268 None:intergenic 45.0%
CCCTCTCGTTGAAAATGAAG+AGG - Chr8:23601761-23601780 None:intergenic 45.0%
CCGAAAGGATTGAGGAAGTT+TGG + Chr8:23602623-23602642 MsG0880043358.01.T01:CDS 45.0%
CGTTGAAAATGAAGAGGAGG+TGG - Chr8:23601755-23601774 None:intergenic 45.0%
CTAAGTCCATCTCCCAGAAA+GGG - Chr8:23602063-23602082 None:intergenic 45.0%
GATAGAGGATACTCCCTCTA+AGG - Chr8:23602350-23602369 None:intergenic 45.0%
GGACGGATAATACCAGGTAA+AGG - Chr8:23602529-23602548 None:intergenic 45.0%
GGCATTCTTTGTCTCTCTCA+AGG + Chr8:23601821-23601840 MsG0880043358.01.T01:CDS 45.0%
GGCTGGATTCCACAATACAA+TGG - Chr8:23601864-23601883 None:intergenic 45.0%
TCCTCCAAGAGTTACTTCTG+AGG - Chr8:23602162-23602181 None:intergenic 45.0%
TCTAAGTCCATCTCCCAGAA+AGG - Chr8:23602064-23602083 None:intergenic 45.0%
TGCTGCAACCGATAAGAAGA+AGG + Chr8:23601469-23601488 MsG0880043358.01.T01:CDS 45.0%
ATTGTGGAATCCAGCCACCA+GGG + Chr8:23601868-23601887 MsG0880043358.01.T01:CDS 50.0%
CAGTGTTCTCGTATGGTCGA+TGG - Chr8:23601916-23601935 None:intergenic 50.0%
GCCTCAGAAGTAACTCTTGG+AGG + Chr8:23602158-23602177 MsG0880043358.01.T01:CDS 50.0%
GCGATAGGACGGATAATACC+AGG - Chr8:23602535-23602554 None:intergenic 50.0%
GTCTTAAATTCCCTGGTGGC+TGG - Chr8:23601881-23601900 None:intergenic 50.0%
TATTGTGGAATCCAGCCACC+AGG + Chr8:23601867-23601886 MsG0880043358.01.T01:CDS 50.0%
TCGTATGGTCGATGGCTACA+AGG - Chr8:23601908-23601927 None:intergenic 50.0%
TGGTCGATGGCTACAAGGAA+CGG - Chr8:23601903-23601922 None:intergenic 50.0%
! GAATAGCCCTTTCTGGGAGA+TGG + Chr8:23602054-23602073 MsG0880043358.01.T01:CDS 50.0%
! TTCTTGGGATGAGCGTTGTC+AGG + Chr8:23602204-23602223 MsG0880043358.01.T01:CDS 50.0%
GGCTTAATGGAGAGCCACGA+AGG + Chr8:23602644-23602663 MsG0880043358.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 23601464 23602723 23601464 ID=MsG0880043358.01;Name=MsG0880043358.01
Chr8 mRNA 23601464 23602723 23601464 ID=MsG0880043358.01.T01;Parent=MsG0880043358.01;Name=MsG0880043358.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|1|2|0|397
Chr8 exon 23601464 23601962 23601464 ID=MsG0880043358.01.T01:exon:8633;Parent=MsG0880043358.01.T01
Chr8 exon 23602029 23602723 23602029 ID=MsG0880043358.01.T01:exon:8634;Parent=MsG0880043358.01.T01
Chr8 CDS 23601464 23601962 23601464 ID=MsG0880043358.01.T01:cds;Parent=MsG0880043358.01.T01
Chr8 CDS 23602029 23602723 23602029 ID=MsG0880043358.01.T01:cds;Parent=MsG0880043358.01.T01
Gene Sequence

>MsG0880043358.01.T01

ATGGCTGCTGCAACCGATAAGAAGAAGGTTAGTACTCCCTCGCATATACACGATGACATTGCTTTCTCTATACTCTCCAAATTGCCTCTTAAATCCCTCACAAGATTCACTTGTGCTAAGAAATCATGGTCTCGTTTATTTCAAAACCCTCATTTCATGAACATGTTTCGCACTAATTTCATATCCAAACATAATGAGGAAGAAGACAGCACGAATCTTCTCCTAAATCATGACGCTTTGTACACATTTTCCGGTGAGAGTTTTGAGAATAGAGTCAGATTAGAGTTGCCACCTCCTCTTCATTTTCAACGAGAGGGTTGTCCATTTATTCGCAATTGGTGTTCAGCTAGTGTTAATGGCATTCTTTGTCTCTCTCAAGGTTTTCTTGATCCCATGACCATTGTATTGTGGAATCCAGCCACCAGGGAATTTAAGACCGTTCCTTGTAGCCATCGACCATACGAGAACACTGAGTTTAATACTCAACCTTTTTCATTTGGTGCTTGGGTAATTTTGCCAGAGAAGAATAGCCCTTTCTGGGAGATGGACTTAGATTTTGATGATAATGATGATTTTTGGGAGGGTGTGCCAATAAATATGTATGACCCTAATTATTGGGAGATATACAGCCTCAGAAGTAACTCTTGGAGGAAACTCAACGATGATGTTATGCATTCTTGGGATGAGCGTTGTCAGGTGAATTTGAATGAATTTTGCCATTGGTTGGGCCTAGATAATGATATGGGGTCGTTTGACTTTATCAATGAGATATTTTTTGTAACAAACTCACCTTCATTTGACTTTAGTAAAGAGACATCCTTAGAGGGAGTATCCTCTATCTATGATTTCAATCGCAGGAAGTTGGAGACAAAATTGGTGGTATTAAATGGTTATGTTGCTTTCATTTGTACTGTCCTCATGACTTCTTGTATTCACATATGGGTTTTGGGTGAACTTGGTGTTAAAGAATCGTGGACAAAACTTTTTGTTGTTGGACCTTTACCTGGTATTATCCGTCCTATCGCAGTAGGAATAAAGAGCATTATCTTCTACATAAAAGAAGATACGGAGTTAGCATGTTTTGATTTAAGTACCGAAAGGATTGAGGAAGTTTGGCTTAATGGAGAGCCACGAAGGTTGCAAATTATAACTTACAAGAAAAACCTTCTTTCTTTTGGAAGAATGAATAGTTAA
Protein sequence

>MsG0880043358.01.T01

MAAATDKKKVSTPSHIHDDIAFSILSKLPLKSLTRFTCAKKSWSRLFQNPHFMNMFRTNFISKHNEEEDSTNLLLNHDALYTFSGESFENRVRLELPPPLHFQREGCPFIRNWCSASVNGILCLSQGFLDPMTIVLWNPATREFKTVPCSHRPYENTEFNTQPFSFGAWVILPEKNSPFWEMDLDFDDNDDFWEGVPINMYDPNYWEIYSLRSNSWRKLNDDVMHSWDERCQVNLNEFCHWLGLDNDMGSFDFINEIFFVTNSPSFDFSKETSLEGVSSIYDFNRRKLETKLVVLNGYVAFICTVLMTSCIHIWVLGELGVKESWTKLFVVGPLPGIIRPIAVGIKSIIFYIKEDTELACFDLSTERIEEVWLNGEPRRLQIITYKKNLLSFGRMNS*