AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039751.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039751.01.T01 MTR_7g079640 88.554 332 37 1 1 332 52 382 0.0 595
MsG0780039751.01.T01 MTR_2g064950 65.753 365 76 8 1 332 49 397 4.27e-149 425
MsG0780039751.01.T01 MTR_8g013000 64.742 329 82 7 1 303 49 369 2.61e-125 365
MsG0780039751.01.T01 MTR_7g069550 52.738 347 123 9 1 315 53 390 2.44e-106 317
MsG0780039751.01.T01 MTR_5g074800 50.862 348 135 10 1 320 1 340 1.20e-99 297
MsG0780039751.01.T01 MTR_5g072060 48.214 336 115 9 1 305 45 352 1.72e-84 267
MsG0780039751.01.T01 MTR_2g104340 46.591 352 149 12 2 322 50 393 4.48e-83 257
MsG0780039751.01.T01 MTR_4g055820 50.323 310 116 9 2 279 54 357 7.27e-82 253
MsG0780039751.01.T01 MTR_8g041270 46.240 359 149 13 2 322 51 403 7.78e-80 249
MsG0780039751.01.T01 MTR_8g063440 44.582 323 150 9 1 305 51 362 1.50e-76 240
MsG0780039751.01.T01 MTR_5g097330 41.888 339 149 11 1 304 55 380 5.14e-75 236
MsG0780039751.01.T01 MTR_6g086825 41.667 360 159 14 1 321 109 456 1.86e-68 221
MsG0780039751.01.T01 MTR_0129s0070 40.854 328 161 9 1 304 56 374 1.99e-68 219
MsG0780039751.01.T01 MTR_8g012090 42.718 309 134 10 1 295 55 334 4.96e-68 216
MsG0780039751.01.T01 MTR_5g097350 41.420 338 161 12 1 315 55 378 8.15e-68 217
MsG0780039751.01.T01 MTR_2g022000 40.688 349 154 11 1 304 55 395 1.67e-67 218
MsG0780039751.01.T01 MTR_2g104280 41.783 359 165 13 2 322 50 402 2.70e-67 217
MsG0780039751.01.T01 MTR_8g464770 41.195 318 161 8 2 304 52 358 3.41e-67 216
MsG0780039751.01.T01 MTR_5g069390 40.336 357 171 16 1 322 65 414 6.62e-67 218
MsG0780039751.01.T01 MTR_5g037680 42.105 342 161 16 1 316 47 377 7.03e-67 217
MsG0780039751.01.T01 MTR_0054s0090 40.000 355 161 14 1 316 56 397 9.51e-67 215
MsG0780039751.01.T01 MTR_5g059430 40.730 356 141 18 1 303 65 403 1.83e-66 215
MsG0780039751.01.T01 MTR_5g097220 39.118 363 153 14 1 316 52 393 2.65e-66 214
MsG0780039751.01.T01 MTR_0129s0120 40.176 341 169 10 1 315 56 387 3.77e-66 213
MsG0780039751.01.T01 MTR_3g011120 41.379 319 157 9 2 304 54 358 1.93e-65 211
MsG0780039751.01.T01 MTR_2g021770 39.535 344 159 12 1 304 51 385 2.50e-64 209
MsG0780039751.01.T01 MTR_5g096630 38.192 343 166 9 1 304 57 392 3.32e-64 209
MsG0780039751.01.T01 MTR_4g102410 39.583 336 164 10 1 304 100 428 6.90e-64 209
MsG0780039751.01.T01 MTR_7g017040 41.379 348 158 12 1 315 55 389 2.66e-63 206
MsG0780039751.01.T01 MTR_0054s0100 38.936 357 164 15 1 316 56 399 8.25e-63 205
MsG0780039751.01.T01 MTR_2g021800 38.567 363 169 11 1 317 55 409 1.28e-62 205
MsG0780039751.01.T01 MTR_2g038680 40.456 351 145 12 1 304 55 388 3.08e-62 204
MsG0780039751.01.T01 MTR_2g034700 42.236 322 148 12 1 304 53 354 9.11e-62 201
MsG0780039751.01.T01 MTR_2g021950 38.304 342 176 10 1 315 55 388 1.30e-61 201
MsG0780039751.01.T01 MTR_7g017130 41.515 330 156 10 1 304 57 375 2.60e-61 201
MsG0780039751.01.T01 MTR_6g090180 40.294 340 158 11 1 304 86 416 3.69e-61 201
MsG0780039751.01.T01 MTR_8g467670 39.820 334 166 11 1 315 53 370 5.25e-61 200
MsG0780039751.01.T01 MTR_6g078010 42.368 321 154 15 1 302 3 311 6.91e-61 198
MsG0780039751.01.T01 MTR_7g017170 41.212 330 157 10 1 304 57 375 7.33e-61 200
MsG0780039751.01.T01 MTR_1g007410 40.179 336 151 11 1 301 61 381 1.24e-60 199
MsG0780039751.01.T01 MTR_0831s0010 40.000 325 160 13 1 304 89 399 2.62e-60 199
MsG0780039751.01.T01 MTR_5g024260 40.361 332 162 9 1 304 61 384 1.61e-59 197
MsG0780039751.01.T01 MTR_4g007940 40.240 333 149 15 12 307 4 323 2.55e-59 194
MsG0780039751.01.T01 MTR_4g117550 37.464 347 160 11 1 322 56 370 2.28e-58 192
MsG0780039751.01.T01 MTR_4g130330 40.476 336 153 15 2 302 43 366 3.16e-58 192
MsG0780039751.01.T01 MTR_5g022850 38.841 345 158 12 10 315 14 344 1.65e-57 190
MsG0780039751.01.T01 MTR_4g028510 40.056 357 166 18 1 322 56 399 6.72e-57 191
MsG0780039751.01.T01 MTR_0134s0060 39.080 348 154 13 2 302 52 388 1.74e-56 189
MsG0780039751.01.T01 MTR_6g478230 39.193 347 176 13 1 321 66 403 2.12e-56 189
MsG0780039751.01.T01 MTR_5g096910 40.690 290 140 10 35 302 89 368 2.76e-56 187
MsG0780039751.01.T01 MTR_2g066170 38.123 341 171 14 1 315 51 377 3.83e-56 187
MsG0780039751.01.T01 MTR_6g079770 39.189 370 166 17 1 322 56 414 7.24e-56 187
MsG0780039751.01.T01 MTR_7g111700 39.117 317 161 11 5 304 43 344 1.36e-55 185
MsG0780039751.01.T01 MTR_6g079810 39.769 347 148 16 1 302 109 439 1.71e-55 187
MsG0780039751.01.T01 MTR_8g064890 37.135 342 172 14 1 316 55 379 1.94e-55 185
MsG0780039751.01.T01 MTR_0129s0050 39.368 348 153 14 1 304 53 386 2.33e-55 186
MsG0780039751.01.T01 MTR_6g080680 40.643 342 163 16 1 315 58 386 4.01e-55 185
MsG0780039751.01.T01 MTR_2g066200 38.551 345 166 15 1 315 51 379 8.07e-55 184
MsG0780039751.01.T01 MTR_7g023800 38.872 337 149 15 1 315 1 302 1.63e-54 181
MsG0780039751.01.T01 MTR_4g028680 38.547 358 169 19 1 322 55 397 7.50e-54 181
MsG0780039751.01.T01 MTR_2g065860 38.012 342 174 12 1 316 51 380 8.18e-54 181
MsG0780039751.01.T01 MTR_6g461660 38.095 336 165 11 1 306 58 380 8.25e-54 181
MsG0780039751.01.T01 MTR_4g028660 38.873 355 167 18 1 320 55 394 1.01e-53 181
MsG0780039751.01.T01 MTR_4g027450 38.873 355 167 18 1 320 55 394 1.01e-53 181
MsG0780039751.01.T01 MTR_8g032980 35.602 382 168 14 1 322 55 418 1.04e-52 179
MsG0780039751.01.T01 MTR_6g090250 37.088 364 154 14 1 302 50 400 1.05e-52 179
MsG0780039751.01.T01 MTR_8g046310 39.130 322 158 13 1 304 55 356 1.98e-52 177
MsG0780039751.01.T01 MTR_6g079790 40.064 312 144 13 47 322 2 306 2.04e-52 175
MsG0780039751.01.T01 MTR_2g065840 36.550 342 178 11 1 315 51 380 2.32e-52 177
MsG0780039751.01.T01 MTR_5g091140 37.690 329 151 7 1 300 87 390 3.01e-52 178
MsG0780039751.01.T01 MTR_2g066150 37.855 317 161 12 1 307 51 341 1.40e-51 174
MsG0780039751.01.T01 MTR_4g014370 36.056 355 159 13 1 304 50 387 1.47e-51 176
MsG0780039751.01.T01 MTR_1g007450 39.104 335 162 12 1 304 51 374 1.92e-51 176
MsG0780039751.01.T01 MTR_8g033140 34.959 369 169 14 1 315 58 409 2.06e-51 176
MsG0780039751.01.T01 MTR_8g036970 37.618 319 143 11 1 310 37 308 2.60e-51 174
MsG0780039751.01.T01 MTR_8g039500 38.365 318 151 13 1 295 51 346 2.98e-51 173
MsG0780039751.01.T01 MTR_7g016930 41.877 277 112 9 1 255 55 304 1.59e-50 171
MsG0780039751.01.T01 MTR_5g068490 40.070 287 144 11 44 322 8 274 8.26e-50 167
MsG0780039751.01.T01 MTR_4g028750 38.166 338 165 14 2 308 56 380 8.73e-50 171
MsG0780039751.01.T01 MTR_5g074850 35.475 358 174 12 1 318 76 416 1.55e-49 177
MsG0780039751.01.T01 MTR_5g064700 35.616 365 179 15 1 315 1 359 2.19e-49 169
MsG0780039751.01.T01 MTR_1g007430 41.503 306 138 10 1 282 35 323 3.04e-49 167
MsG0780039751.01.T01 MTR_5g043605 38.333 300 154 14 41 322 50 336 6.63e-49 168
MsG0780039751.01.T01 MTR_8g064790 37.152 323 147 15 1 315 54 328 1.36e-48 166
MsG0780039751.01.T01 MTR_8g063350 34.539 304 167 11 35 316 45 338 1.61e-48 166
MsG0780039751.01.T01 MTR_6g087620 38.255 298 148 12 35 315 1 279 2.00e-48 164
MsG0780039751.01.T01 MTR_8g033000 34.759 374 176 14 1 322 3 360 6.88e-48 165
MsG0780039751.01.T01 MTR_5g092860 34.262 359 157 13 1 316 41 363 1.32e-47 164
MsG0780039751.01.T01 MTR_5g097680 36.290 372 171 16 1 315 56 418 1.75e-47 166
MsG0780039751.01.T01 MTR_1g050312 37.979 287 132 12 35 304 16 273 1.64e-45 157
MsG0780039751.01.T01 MTR_5g086590 39.655 232 118 8 79 298 10 231 2.03e-45 155
MsG0780039751.01.T01 MTR_5g075370 34.884 344 168 11 1 303 49 377 3.59e-45 164
MsG0780039751.01.T01 MTR_6g004450 46.479 213 79 8 111 301 45 244 7.09e-45 154
MsG0780039751.01.T01 MTR_1g053510 33.065 372 179 12 1 323 54 404 1.30e-44 157
MsG0780039751.01.T01 MTR_8g063330 36.964 303 163 10 1 281 53 349 1.92e-44 158
MsG0780039751.01.T01 MTR_8g046200 33.777 376 168 12 5 315 54 413 4.10e-44 156
MsG0780039751.01.T01 MTR_1g098180 34.844 353 152 16 1 322 58 363 4.29e-44 155
MsG0780039751.01.T01 MTR_8g033110 32.787 366 183 12 1 315 53 406 1.16e-43 155
MsG0780039751.01.T01 MTR_2g021920 40.171 234 101 7 115 315 37 264 6.03e-43 149
MsG0780039751.01.T01 MTR_2g104260 41.126 231 97 8 2 196 50 277 1.70e-41 146
MsG0780039751.01.T01 MTR_4g016000 40.598 234 97 10 87 300 2 213 8.20e-41 142
MsG0780039751.01.T01 MTR_5g092850 33.429 347 167 11 1 305 55 379 2.11e-40 146
MsG0780039751.01.T01 MTR_8g063370 32.153 339 188 13 1 315 63 383 6.79e-40 145
MsG0780039751.01.T01 MTR_8g063310 32.544 338 192 15 5 315 60 388 1.23e-39 144
MsG0780039751.01.T01 MTR_5g098950 35.401 274 120 7 30 282 36 273 1.24e-38 138
MsG0780039751.01.T01 MTR_5g097820 39.048 210 104 8 1 188 62 269 3.57e-38 137
MsG0780039751.01.T01 MTR_5g076490 34.444 360 169 15 1 322 3 333 3.68e-38 139
MsG0780039751.01.T01 MTR_8g046220 32.479 351 168 10 1 304 56 384 5.17e-38 140
MsG0780039751.01.T01 MTR_6g090260 42.995 207 103 9 102 302 41 238 7.50e-38 136
MsG0780039751.01.T01 MTR_8g033090 30.790 367 186 14 1 315 53 403 1.46e-37 139
MsG0780039751.01.T01 MTR_8g018110 33.149 362 169 14 1 315 53 388 3.81e-37 137
MsG0780039751.01.T01 MTR_4g014370 33.123 317 137 11 1 304 61 315 9.95e-37 135
MsG0780039751.01.T01 MTR_5g097850 36.029 272 123 9 56 316 5 236 8.01e-36 130
MsG0780039751.01.T01 MTR_5g023970 41.618 173 91 5 135 304 8 173 3.00e-34 124
MsG0780039751.01.T01 MTR_7g104710 41.016 256 108 12 1 239 56 285 9.46e-34 126
MsG0780039751.01.T01 MTR_1g050308 32.229 332 148 14 1 318 73 341 1.32e-33 127
MsG0780039751.01.T01 MTR_5g096900 37.956 274 132 12 1 247 51 313 1.51e-33 126
MsG0780039751.01.T01 MTR_7g019250 38.095 231 97 9 96 303 206 413 1.99e-33 129
MsG0780039751.01.T01 MTR_5g066420 47.917 144 64 6 169 306 2 140 4.80e-32 117
MsG0780039751.01.T01 MTR_3g018750 34.934 229 96 7 115 304 3 217 5.74e-31 117
MsG0780039751.01.T01 MTR_6g009350 39.500 200 84 9 115 289 8 195 3.28e-30 114
MsG0780039751.01.T01 MTR_5g096920 42.953 149 76 3 169 315 2 143 1.49e-28 108
MsG0780039751.01.T01 MTR_6g070880 36.269 193 82 8 1 157 71 258 4.35e-27 107
MsG0780039751.01.T01 MTR_6g071420 36.269 193 82 8 1 157 71 258 4.35e-27 107
MsG0780039751.01.T01 MTR_7g101200 35.909 220 95 8 81 282 37 228 1.00e-26 105
MsG0780039751.01.T01 MTR_8g035690 33.455 275 120 12 96 322 41 300 1.90e-26 106
MsG0780039751.01.T01 MTR_3g030620 43.478 161 80 7 169 322 24 180 2.06e-26 103
MsG0780039751.01.T01 MTR_5g086780 32.727 220 90 6 84 302 66 228 3.53e-26 104
MsG0780039751.01.T01 MTR_5g097540 29.617 287 140 10 1 282 3 232 9.60e-26 104
MsG0780039751.01.T01 MTR_6g079730 52.101 119 51 4 184 302 14 126 4.20e-25 99.0
MsG0780039751.01.T01 MTR_1g076810 37.143 175 91 5 1 158 57 229 4.34e-25 106
MsG0780039751.01.T01 MTR_7g017150 42.690 171 82 8 138 302 82 242 1.06e-24 101
MsG0780039751.01.T01 MTR_7g017120 42.105 171 83 8 138 302 81 241 1.88e-24 100
MsG0780039751.01.T01 MTR_5g091150 37.647 170 82 6 62 230 38 184 7.30e-24 97.4
MsG0780039751.01.T01 MTR_2g022020 45.794 107 53 3 212 315 7 111 7.97e-23 92.0
MsG0780039751.01.T01 MTR_5g037190 33.484 221 93 7 1 191 55 251 1.26e-22 95.1
MsG0780039751.01.T01 MTR_1g047080 28.940 349 150 16 1 322 3 280 1.35e-21 93.2
MsG0780039751.01.T01 MTR_8g073570 40.157 127 70 5 1 126 49 170 5.18e-20 86.3
MsG0780039751.01.T01 MTR_5g036250 42.991 107 59 2 216 322 4 108 9.85e-20 85.9
MsG0780039751.01.T01 MTR_2g026010 25.796 314 181 11 31 301 39 343 5.77e-19 87.4
MsG0780039751.01.T01 MTR_5g094350 31.405 242 102 11 1 200 52 271 9.68e-19 85.1
MsG0780039751.01.T01 MTR_5g049560 37.838 148 75 5 1 132 3 149 2.38e-18 84.0
MsG0780039751.01.T01 MTR_4g133170 25.228 329 198 11 5 309 39 343 2.50e-18 85.5
MsG0780039751.01.T01 MTR_5g097660 48.780 82 41 1 220 301 15 95 1.84e-17 77.8
MsG0780039751.01.T01 MTR_4g023130 28.512 242 143 10 85 315 79 301 3.61e-16 78.2
MsG0780039751.01.T01 MTR_4g023080 28.099 242 144 10 85 315 120 342 8.52e-16 77.8
MsG0780039751.01.T01 MTR_2g031640 26.846 298 174 14 35 295 45 335 1.45e-15 77.4
MsG0780039751.01.T01 MTR_6g465360 34.694 147 72 6 1 130 101 240 5.61e-15 74.7
MsG0780039751.01.T01 MTR_2g031720 25.000 264 166 9 61 301 91 345 6.50e-15 75.5
MsG0780039751.01.T01 MTR_5g097650 40.909 110 52 4 2 110 62 159 1.35e-14 70.9
MsG0780039751.01.T01 MTR_2g028130 25.455 275 164 12 69 309 107 374 2.31e-14 73.9
MsG0780039751.01.T01 MTR_1g068875 25.159 314 196 12 5 298 39 333 3.16e-14 73.2
MsG0780039751.01.T01 MTR_1g097560 27.935 247 145 11 69 303 132 357 4.02e-14 72.8
MsG0780039751.01.T01 MTR_8g101580 29.130 230 132 12 62 276 136 349 5.46e-14 72.8
MsG0780039751.01.T01 MTR_1g050305 47.126 87 39 4 225 306 21 105 5.59e-14 68.9
MsG0780039751.01.T01 MTR_5g022420 26.357 258 149 12 56 296 112 345 1.32e-13 71.2
MsG0780039751.01.T01 MTR_3g030350 25.541 231 147 8 53 272 113 329 1.68e-13 71.2
MsG0780039751.01.T01 MTR_4g117720 28.082 292 166 14 37 300 68 343 1.76e-13 70.9
MsG0780039751.01.T01 MTR_2g425980 25.237 317 179 14 35 298 44 355 4.51e-13 70.1
MsG0780039751.01.T01 MTR_5g096050 41.964 112 47 5 169 267 2 108 4.75e-13 65.5
MsG0780039751.01.T01 MTR_2g028160 24.820 278 163 12 69 309 107 375 2.10e-12 67.8
MsG0780039751.01.T01 MTR_4g023200 25.767 326 211 14 6 315 38 348 2.26e-12 67.4
MsG0780039751.01.T01 MTR_4g094400 42.157 102 47 3 169 258 11 112 2.27e-12 63.9
MsG0780039751.01.T01 MTR_4g070550 24.910 277 162 12 69 306 102 371 2.42e-12 67.8
MsG0780039751.01.T01 MTR_5g057790 24.151 265 166 10 17 265 91 336 4.49e-12 67.0
MsG0780039751.01.T01 MTR_4g021785 25.514 243 150 9 85 315 120 343 5.39e-12 66.2
MsG0780039751.01.T01 MTR_4g116330 27.798 277 161 14 50 307 100 356 7.79e-12 66.2
MsG0780039751.01.T01 MTR_0667s0010 26.159 302 168 17 50 307 72 362 7.93e-12 66.2
MsG0780039751.01.T01 MTR_3g036290 28.431 204 113 9 69 264 120 298 8.00e-12 65.9
MsG0780039751.01.T01 MTR_3g007360 27.803 223 115 10 69 269 146 344 8.75e-12 65.9
MsG0780039751.01.T01 MTR_4g023190 25.076 331 206 14 6 315 38 347 1.14e-11 65.5
MsG0780039751.01.T01 MTR_3g007410 27.803 223 115 10 69 269 146 344 1.22e-11 65.5
MsG0780039751.01.T01 MTR_5g057710 23.396 265 168 11 17 265 91 336 1.48e-11 65.5
MsG0780039751.01.T01 MTR_2g021150 25.564 266 158 10 69 318 116 357 1.60e-11 65.1
MsG0780039751.01.T01 MTR_4g117430 36.937 111 58 5 85 189 16 120 1.89e-11 62.0
MsG0780039751.01.T01 MTR_5g096070 37.255 102 54 3 25 123 65 159 2.27e-11 62.0
MsG0780039751.01.T01 MTR_4g023150 25.166 302 187 12 33 315 67 348 2.38e-11 64.3
MsG0780039751.01.T01 MTR_3g058480 27.136 199 105 8 12 189 92 271 2.43e-11 64.3
MsG0780039751.01.T01 MTR_2g029990 24.434 221 134 8 54 259 136 338 3.69e-11 63.9
MsG0780039751.01.T01 MTR_4g023380 25.325 308 184 13 36 315 76 365 3.99e-11 63.9
MsG0780039751.01.T01 MTR_5g070080 24.609 256 166 7 59 300 114 356 4.01e-11 63.9
MsG0780039751.01.T01 MTR_3g047540 36.782 87 51 1 138 224 6 88 4.50e-11 58.9
MsG0780039751.01.T01 MTR_6g060650 28.340 247 144 11 73 299 110 343 6.52e-11 63.2
MsG0780039751.01.T01 MTR_1g097330 26.749 243 147 11 69 294 110 338 7.44e-11 63.2
MsG0780039751.01.T01 MTR_5g094360 39.773 88 32 5 92 159 40 126 7.47e-11 60.1
MsG0780039751.01.T01 MTR_4g118380 30.040 253 156 11 62 299 108 354 7.58e-11 62.8
MsG0780039751.01.T01 MTR_4g117850 27.559 254 144 11 85 315 132 368 7.62e-11 63.2
MsG0780039751.01.T01 MTR_6g048120 26.126 222 136 9 52 259 123 330 8.79e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039751.01.T01 AT4G12560 25.342 292 166 13 57 309 79 357 3.89e-16 79.3
MsG0780039751.01.T01 AT4G12560 25.342 292 166 13 57 309 79 357 3.89e-16 79.3
MsG0780039751.01.T01 AT4G12560 25.342 292 166 13 57 309 79 357 3.89e-16 79.3
MsG0780039751.01.T01 AT4G22390 24.476 286 171 13 50 304 78 349 9.66e-12 65.9
MsG0780039751.01.T01 AT4G22390 24.476 286 171 13 50 304 78 349 1.04e-11 65.9

Find 69 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTAATATGTGATCTATTA+TGG 0.152705 7:-68622506 None:intergenic
CTTTGAGAATAGACTAAAAT+TGG 0.211718 7:+68622033 MsG0780039751.01.T01:CDS
GCTAGAATCCAAAATATAAA+AGG 0.243589 7:-68622089 None:intergenic
TTGTAAGTTATTCTATTTGA+TGG 0.273402 7:-68622826 None:intergenic
GTCGAAATTATCCTAAAGTA+TGG 0.275940 7:+68622299 MsG0780039751.01.T01:CDS
AAGGCAAAGTGTTCCGTTAA+TGG 0.334143 7:-68622113 None:intergenic
TATGAGGATCCCAATGATTA+TGG 0.352679 7:+68622529 MsG0780039751.01.T01:CDS
CTATAAATCTCCCATACTTT+AGG 0.356787 7:-68622310 None:intergenic
ACTAGACAGTAAATGCAAAA+AGG 0.361358 7:-68622005 None:intergenic
TGGACTAGACTCTTCACTAT+TGG 0.377675 7:+68622664 MsG0780039751.01.T01:CDS
TCCTATATCGAGGAACCTAT+TGG 0.386230 7:+68622697 MsG0780039751.01.T01:CDS
TCTAATATGTGATCTATTAT+GGG 0.390101 7:-68622505 None:intergenic
TTGGATTCTAGCACCATTAA+CGG 0.405104 7:+68622100 MsG0780039751.01.T01:CDS
GCACTATTGGTGATGTTAAA+CGG 0.408278 7:+68622559 MsG0780039751.01.T01:CDS
TGCCGCCATACCATAATCAT+TGG 0.408627 7:-68622539 None:intergenic
AGTATTTCATCACCATGTTC+AGG 0.412911 7:+68621894 None:intergenic
CCATTCCTCCCTACTCCAAT+AGG 0.426857 7:-68622712 None:intergenic
CACTATTGGTGATGTTAAAC+GGG 0.433911 7:+68622560 MsG0780039751.01.T01:CDS
AGGAATGTCATTGACTTCAT+TGG 0.434102 7:-68622176 None:intergenic
GAATTCGGTTACACATTAGT+TGG 0.437234 7:+68622865 MsG0780039751.01.T01:CDS
CTAGAATCCAAAATATAAAA+GGG 0.444875 7:-68622088 None:intergenic
ACACACATGGTTGAGGAAAT+CGG 0.454794 7:+68622790 MsG0780039751.01.T01:CDS
TTATGGTATGGCGGCACTAT+TGG 0.458031 7:+68622546 MsG0780039751.01.T01:CDS
GAAATAATAGAGCTCCAATC+AGG 0.461115 7:-68622223 None:intergenic
GATCTATTAAACACGAATGA+TGG 0.462837 7:+68622394 MsG0780039751.01.T01:CDS
TCCAATAGGTTCCTCGATAT+AGG 0.466442 7:-68622698 None:intergenic
AGATCACATATTAGATTATG+AGG 0.470272 7:+68622513 MsG0780039751.01.T01:CDS
TGGAGTCTGTGTCACCTGAT+TGG 0.470650 7:+68622209 MsG0780039751.01.T01:CDS
TGACATTCCTCCCAGTCCTA+TGG 0.474124 7:+68622189 MsG0780039751.01.T01:CDS
GCGATAAAACATTTGTATTG+TGG 0.482872 7:+68622143 MsG0780039751.01.T01:CDS
GAGTGATGTATCATCATAGT+AGG 0.484957 7:-68621942 None:intergenic
GTATTTCATCACCATGTTCA+GGG 0.502151 7:+68621895 None:intergenic
CTTGCCAACTCCACTTCAAA+CGG 0.502173 7:+68622057 MsG0780039751.01.T01:CDS
AACTGATGATCAACTTAGCT+TGG 0.505158 7:+68622438 MsG0780039751.01.T01:CDS
TCGAGGAACCTATTGGAGTA+GGG 0.512226 7:+68622704 MsG0780039751.01.T01:CDS
AACTTGGTGTGAGTGAATCA+TGG 0.519514 7:+68622644 MsG0780039751.01.T01:CDS
GCCGCCATACCATAATCATT+GGG 0.522887 7:-68622538 None:intergenic
ATACACCAGCTTGTGCATAA+AGG 0.528233 7:+68621968 MsG0780039751.01.T01:CDS
TGACACAGACTCCATAGGAC+TGG 0.530406 7:-68622200 None:intergenic
TCATTGACTTCATTGGTTGT+TGG 0.532657 7:-68622169 None:intergenic
CGAATGATGGAATATGTCAT+TGG 0.534886 7:+68622407 MsG0780039751.01.T01:CDS
TGCTCCGTTTGAAGTGGAGT+TGG 0.538804 7:-68622061 None:intergenic
CCTATTGGAGTAGGGAGGAA+TGG 0.540683 7:+68622712 MsG0780039751.01.T01:CDS
TAGAATCCAAAATATAAAAG+GGG 0.542524 7:-68622087 None:intergenic
TTTAAGTACACACATGGTTG+AGG 0.553483 7:+68622783 MsG0780039751.01.T01:CDS
ATCGAGGAACCTATTGGAGT+AGG 0.565568 7:+68622703 MsG0780039751.01.T01:CDS
TCGAAATTATCCTAAAGTAT+GGG 0.571607 7:+68622300 MsG0780039751.01.T01:CDS
AATAGGTTCCTCGATATAGG+AGG 0.574393 7:-68622695 None:intergenic
GGATCCCAATGATTATGGTA+TGG 0.579920 7:+68622534 MsG0780039751.01.T01:CDS
ACCTAAAATTAATATGTCAA+AGG 0.580043 7:-68622620 None:intergenic
CTATGATTTAAGTACACACA+TGG 0.582076 7:+68622777 MsG0780039751.01.T01:CDS
ATAGTCTAAGATGTAACACT+TGG 0.589208 7:+68622329 MsG0780039751.01.T01:CDS
TCAGGTGACACAGACTCCAT+AGG 0.598844 7:-68622205 None:intergenic
TGCGACCTTTATGCACAAGC+TGG 0.599923 7:-68621973 None:intergenic
AGAATCCAAAATATAAAAGG+GGG 0.600061 7:-68622086 None:intergenic
GGAACACTTTGCCTTTCCAA+AGG 0.601813 7:+68622121 MsG0780039751.01.T01:CDS
GACACAGACTCCATAGGACT+GGG 0.613261 7:-68622199 None:intergenic
AATCCAAAATATAAAAGGGG+GGG 0.615274 7:-68622084 None:intergenic
TCCTCGATATAGGAGGGCAG+AGG 0.626531 7:-68622688 None:intergenic
GAATCCAAAATATAAAAGGG+GGG 0.631039 7:-68622085 None:intergenic
ATACGAAGAGATCAAAGCGA+TGG 0.636805 7:-68622584 None:intergenic
TCCCAATGATTATGGTATGG+CGG 0.641490 7:+68622537 MsG0780039751.01.T01:CDS
ACCTCTGCCCTCCTATATCG+AGG 0.655488 7:+68622687 MsG0780039751.01.T01:CDS
ATAGGTTCCTCGATATAGGA+GGG 0.657135 7:-68622694 None:intergenic
TATGAAATTATCCCTGAACA+TGG 0.673776 7:-68621906 None:intergenic
TCTTATAGTCATCTCTAACA+CGG 0.682948 7:-68622267 None:intergenic
AGGAACCTATTGGAGTAGGG+AGG 0.683456 7:+68622707 MsG0780039751.01.T01:CDS
TCTCTGTAGTGTGAACGCGA+AGG 0.706159 7:-68622373 None:intergenic
ACAGACTCCATAGGACTGGG+AGG 0.718285 7:-68622196 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACCTAAAATTAATATGTCAA+AGG - Chr7:68622623-68622642 None:intergenic 20.0%
!! ATCTAATATGTGATCTATTA+TGG - Chr7:68622509-68622528 None:intergenic 20.0%
!! CTAGAATCCAAAATATAAAA+GGG - Chr7:68622091-68622110 None:intergenic 20.0%
!! TAGAATCCAAAATATAAAAG+GGG - Chr7:68622090-68622109 None:intergenic 20.0%
!! TCTAATATGTGATCTATTAT+GGG - Chr7:68622508-68622527 None:intergenic 20.0%
!! TTGTAAGTTATTCTATTTGA+TGG - Chr7:68622829-68622848 None:intergenic 20.0%
!!! ACCTTTGACATATTAATTTT+AGG + Chr7:68622619-68622638 MsG0780039751.01.T01:CDS 20.0%
!!! ATATTAATTTTAGGTGAACT+TGG + Chr7:68622628-68622647 MsG0780039751.01.T01:CDS 20.0%
!!! ATTATTTCTTTTCATGGATT+TGG + Chr7:68622238-68622257 MsG0780039751.01.T01:CDS 20.0%
!!! ATTTTCCATCGTTTATTTTT+TGG - Chr7:68622750-68622769 None:intergenic 20.0%
! AAAAAGTGGTTTATCGAATT+CGG + Chr7:68622850-68622869 MsG0780039751.01.T01:CDS 25.0%
! AGAATCCAAAATATAAAAGG+GGG - Chr7:68622089-68622108 None:intergenic 25.0%
! AGATCACATATTAGATTATG+AGG + Chr7:68622513-68622532 MsG0780039751.01.T01:CDS 25.0%
! GAATAACTTACAAGAAAAAG+TGG + Chr7:68622836-68622855 MsG0780039751.01.T01:CDS 25.0%
! GCTAGAATCCAAAATATAAA+AGG - Chr7:68622092-68622111 None:intergenic 25.0%
! TCGAAATTATCCTAAAGTAT+GGG + Chr7:68622300-68622319 MsG0780039751.01.T01:CDS 25.0%
! TTCTTCCAAAAAATAAACGA+TGG + Chr7:68622742-68622761 MsG0780039751.01.T01:CDS 25.0%
!! CTTTGAGAATAGACTAAAAT+TGG + Chr7:68622033-68622052 MsG0780039751.01.T01:CDS 25.0%
!!! AGCTCTATTATTTCTTTTCA+TGG + Chr7:68622232-68622251 MsG0780039751.01.T01:CDS 25.0%
AATCCAAAATATAAAAGGGG+GGG - Chr7:68622087-68622106 None:intergenic 30.0%
ACTAGACAGTAAATGCAAAA+AGG - Chr7:68622008-68622027 None:intergenic 30.0%
CTATAAATCTCCCATACTTT+AGG - Chr7:68622313-68622332 None:intergenic 30.0%
CTATGATTTAAGTACACACA+TGG + Chr7:68622777-68622796 MsG0780039751.01.T01:CDS 30.0%
GAATCCAAAATATAAAAGGG+GGG - Chr7:68622088-68622107 None:intergenic 30.0%
GATCTATTAAACACGAATGA+TGG + Chr7:68622394-68622413 MsG0780039751.01.T01:CDS 30.0%
GCGATAAAACATTTGTATTG+TGG + Chr7:68622143-68622162 MsG0780039751.01.T01:CDS 30.0%
GTCGAAATTATCCTAAAGTA+TGG + Chr7:68622299-68622318 MsG0780039751.01.T01:CDS 30.0%
TCTTATAGTCATCTCTAACA+CGG - Chr7:68622270-68622289 None:intergenic 30.0%
! ATAGTCTAAGATGTAACACT+TGG + Chr7:68622329-68622348 MsG0780039751.01.T01:CDS 30.0%
! TACAAATGTTTTATCGCCTT+TGG - Chr7:68622140-68622159 None:intergenic 30.0%
AACTGATGATCAACTTAGCT+TGG + Chr7:68622438-68622457 MsG0780039751.01.T01:CDS 35.0%
GAAATAATAGAGCTCCAATC+AGG - Chr7:68622226-68622245 None:intergenic 35.0%
GAATTCGGTTACACATTAGT+TGG + Chr7:68622865-68622884 MsG0780039751.01.T01:CDS 35.0%
GAGTGATGTATCATCATAGT+AGG - Chr7:68621945-68621964 None:intergenic 35.0%
TATGAGGATCCCAATGATTA+TGG + Chr7:68622529-68622548 MsG0780039751.01.T01:CDS 35.0%
TTAAAAACACATAGCTGCTG+AGG - Chr7:68622474-68622493 None:intergenic 35.0%
TTGGATTCTAGCACCATTAA+CGG + Chr7:68622100-68622119 MsG0780039751.01.T01:CDS 35.0%
TTTAAGTACACACATGGTTG+AGG + Chr7:68622783-68622802 MsG0780039751.01.T01:CDS 35.0%
! CGAATGATGGAATATGTCAT+TGG + Chr7:68622407-68622426 MsG0780039751.01.T01:CDS 35.0%
!! AGGAATGTCATTGACTTCAT+TGG - Chr7:68622179-68622198 None:intergenic 35.0%
!! ATGTTTTATCGCCTTTGGAA+AGG - Chr7:68622135-68622154 None:intergenic 35.0%
!! CACTATTGGTGATGTTAAAC+GGG + Chr7:68622560-68622579 MsG0780039751.01.T01:CDS 35.0%
!! GCACTATTGGTGATGTTAAA+CGG + Chr7:68622559-68622578 MsG0780039751.01.T01:CDS 35.0%
!! TCATTGACTTCATTGGTTGT+TGG - Chr7:68622172-68622191 None:intergenic 35.0%
AATAGGTTCCTCGATATAGG+AGG - Chr7:68622698-68622717 None:intergenic 40.0%
ACACACATGGTTGAGGAAAT+CGG + Chr7:68622790-68622809 MsG0780039751.01.T01:CDS 40.0%
ATACACCAGCTTGTGCATAA+AGG + Chr7:68621968-68621987 MsG0780039751.01.T01:CDS 40.0%
ATACGAAGAGATCAAAGCGA+TGG - Chr7:68622587-68622606 None:intergenic 40.0%
ATAGGTTCCTCGATATAGGA+GGG - Chr7:68622697-68622716 None:intergenic 40.0%
GGATCCCAATGATTATGGTA+TGG + Chr7:68622534-68622553 MsG0780039751.01.T01:CDS 40.0%
TCCAATAGGTTCCTCGATAT+AGG - Chr7:68622701-68622720 None:intergenic 40.0%
TCCCAATGATTATGGTATGG+CGG + Chr7:68622537-68622556 MsG0780039751.01.T01:CDS 40.0%
TCCTATATCGAGGAACCTAT+TGG + Chr7:68622697-68622716 MsG0780039751.01.T01:CDS 40.0%
! AAGGCAAAGTGTTCCGTTAA+TGG - Chr7:68622116-68622135 None:intergenic 40.0%
! TGGACTAGACTCTTCACTAT+TGG + Chr7:68622664-68622683 MsG0780039751.01.T01:CDS 40.0%
!! AACTTGGTGTGAGTGAATCA+TGG + Chr7:68622644-68622663 MsG0780039751.01.T01:CDS 40.0%
ATCGAGGAACCTATTGGAGT+AGG + Chr7:68622703-68622722 MsG0780039751.01.T01:CDS 45.0%
CTTGCCAACTCCACTTCAAA+CGG + Chr7:68622057-68622076 MsG0780039751.01.T01:CDS 45.0%
GCCGCCATACCATAATCATT+GGG - Chr7:68622541-68622560 None:intergenic 45.0%
GGAACACTTTGCCTTTCCAA+AGG + Chr7:68622121-68622140 MsG0780039751.01.T01:CDS 45.0%
TCGAGGAACCTATTGGAGTA+GGG + Chr7:68622704-68622723 MsG0780039751.01.T01:CDS 45.0%
TGCCGCCATACCATAATCAT+TGG - Chr7:68622542-68622561 None:intergenic 45.0%
TTATGGTATGGCGGCACTAT+TGG + Chr7:68622546-68622565 MsG0780039751.01.T01:CDS 45.0%
! GCACCCCCCCTTTTATATTT+TGG + Chr7:68622081-68622100 MsG0780039751.01.T01:CDS 45.0%
AGGAACCTATTGGAGTAGGG+AGG + Chr7:68622707-68622726 MsG0780039751.01.T01:CDS 50.0%
CCATTCCTCCCTACTCCAAT+AGG - Chr7:68622715-68622734 None:intergenic 50.0%
CCTATTGGAGTAGGGAGGAA+TGG + Chr7:68622712-68622731 MsG0780039751.01.T01:CDS 50.0%
GACACAGACTCCATAGGACT+GGG - Chr7:68622202-68622221 None:intergenic 50.0%
TCAGGTGACACAGACTCCAT+AGG - Chr7:68622208-68622227 None:intergenic 50.0%
TCTCTGTAGTGTGAACGCGA+AGG - Chr7:68622376-68622395 None:intergenic 50.0%
TGACACAGACTCCATAGGAC+TGG - Chr7:68622203-68622222 None:intergenic 50.0%
TGACATTCCTCCCAGTCCTA+TGG + Chr7:68622189-68622208 MsG0780039751.01.T01:CDS 50.0%
TGCGACCTTTATGCACAAGC+TGG - Chr7:68621976-68621995 None:intergenic 50.0%
TGGAGTCTGTGTCACCTGAT+TGG + Chr7:68622209-68622228 MsG0780039751.01.T01:CDS 50.0%
! TGCTCCGTTTGAAGTGGAGT+TGG - Chr7:68622064-68622083 None:intergenic 50.0%
ACAGACTCCATAGGACTGGG+AGG - Chr7:68622199-68622218 None:intergenic 55.0%
ACCTCTGCCCTCCTATATCG+AGG + Chr7:68622687-68622706 MsG0780039751.01.T01:CDS 55.0%
TCCTCGATATAGGAGGGCAG+AGG - Chr7:68622691-68622710 None:intergenic 55.0%
!! GGGGGGTGCTCCGTTTGAAG+TGG - Chr7:68622070-68622089 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 68621908 68622906 68621908 ID=MsG0780039751.01;Name=MsG0780039751.01
Chr7 mRNA 68621908 68622906 68621908 ID=MsG0780039751.01.T01;Parent=MsG0780039751.01;Name=MsG0780039751.01.T01;_AED=0.15;_eAED=0.15;_QI=0|-1|0|1|-1|1|1|0|332
Chr7 exon 68621908 68622906 68621908 ID=MsG0780039751.01.T01:exon:11974;Parent=MsG0780039751.01.T01
Chr7 CDS 68621908 68622906 68621908 ID=MsG0780039751.01.T01:cds;Parent=MsG0780039751.01.T01
Gene Sequence

>MsG0780039751.01.T01

ATGTTCAGGGATAATTTCATATCTATTTCTCACTCCTACTATGATGATACATCACTCATCATACACCAGCTTGTGCATAAAGGTCGCAGCACTGTGTCCTTTTTGCATTTACTGTCTAGTCAGAGCTTTGAGAATAGACTAAAATTGGACTTGCCAACTCCACTTCAAACGGAGCACCCCCCCTTTTATATTTTGGATTCTAGCACCATTAACGGAACACTTTGCCTTTCCAAAGGCGATAAAACATTTGTATTGTGGAATCCAACAACCAATGAAGTCAATGACATTCCTCCCAGTCCTATGGAGTCTGTGTCACCTGATTGGAGCTCTATTATTTCTTTTCATGGATTTGGTTATGACCGTGTTAGAGATGACTATAAGATTATTAAGTGTCGAAATTATCCTAAAGTATGGGAGATTTATAGTCTAAGATGTAACACTTGGAAAAAACTTGATGTCAATATGCCTTCGCGTTCACACTACAGAGATCTATTAAACACGAATGATGGAATATGTCATTGGTTGTGTGAAACTGATGATCAACTTAGCTTGGTATCATTTGACCTCAGCAGCTATGTGTTTTTAACAACAAGCACGCCCATAATAGATCACATATTAGATTATGAGGATCCCAATGATTATGGTATGGCGGCACTATTGGTGATGTTAAACGGGTCCATCGCTTTGATCTCTTCGTATGTATGCAAGACTACCTTTGACATATTAATTTTAGGTGAACTTGGTGTGAGTGAATCATGGACTAGACTCTTCACTATTGGACCTCTGCCCTCCTATATCGAGGAACCTATTGGAGTAGGGAGGAATGGTGATATATTCTTCCAAAAAATAAACGATGGAAAATTAGTCTGCTATGATTTAAGTACACACATGGTTGAGGAAATCGGTCTTGAAGAAGCACCATCAAATAGAATAACTTACAAGAAAAAGTGGTTTATCGAATTCGGTTACACATTAGTTGGTTTTCTTGTTCTCAACTAA
Protein sequence

>MsG0780039751.01.T01

MFRDNFISISHSYYDDTSLIIHQLVHKGRSTVSFLHLLSSQSFENRLKLDLPTPLQTEHPPFYILDSSTINGTLCLSKGDKTFVLWNPTTNEVNDIPPSPMESVSPDWSSIISFHGFGYDRVRDDYKIIKCRNYPKVWEIYSLRCNTWKKLDVNMPSRSHYRDLLNTNDGICHWLCETDDQLSLVSFDLSSYVFLTTSTPIIDHILDYEDPNDYGMAALLVMLNGSIALISSYVCKTTFDILILGELGVSESWTRLFTIGPLPSYIEEPIGVGRNGDIFFQKINDGKLVCYDLSTHMVEEIGLEEAPSNRITYKKKWFIEFGYTLVGFLVLN*