AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001954.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001954.01.T01 MTR_1g047080 59.105 313 32 5 84 362 34 284 2.04e-110 324
MsG0180001954.01.T01 MTR_8g046220 45.426 317 123 10 84 357 93 402 3.64e-68 220
MsG0180001954.01.T01 MTR_8g046310 48.148 297 117 11 84 357 92 374 4.07e-67 216
MsG0180001954.01.T01 MTR_8g046200 44.838 339 114 14 84 357 88 418 4.89e-67 218
MsG0180001954.01.T01 MTR_1g053510 42.405 316 134 10 84 357 93 402 8.56e-65 211
MsG0180001954.01.T01 MTR_8g033000 41.916 334 133 8 85 361 34 363 1.91e-63 206
MsG0180001954.01.T01 MTR_8g035690 53.738 214 89 5 154 360 92 302 7.74e-63 203
MsG0180001954.01.T01 MTR_8g033140 42.378 328 133 11 84 361 97 418 1.09e-61 203
MsG0180001954.01.T01 MTR_8g032980 41.888 339 134 10 84 361 85 421 3.66e-61 202
MsG0180001954.01.T01 MTR_8g033090 40.122 329 139 10 84 361 91 412 3.03e-58 194
MsG0180001954.01.T01 MTR_8g033110 39.940 333 137 11 84 361 91 415 1.69e-55 187
MsG0180001954.01.T01 MTR_4g028510 42.188 320 133 13 84 361 93 402 5.77e-55 187
MsG0180001954.01.T01 MTR_8g018110 37.771 323 140 9 84 361 91 397 1.95e-53 181
MsG0180001954.01.T01 MTR_4g028680 39.297 313 151 10 84 361 92 400 4.29e-51 175
MsG0180001954.01.T01 MTR_6g080680 40.717 307 140 12 81 355 93 389 5.40e-50 172
MsG0180001954.01.T01 MTR_5g037680 39.867 301 149 11 81 355 86 380 1.80e-49 172
MsG0180001954.01.T01 MTR_4g130330 39.319 323 143 15 83 361 78 391 4.45e-48 167
MsG0180001954.01.T01 MTR_8g464770 37.457 291 157 8 84 355 90 374 1.22e-46 163
MsG0180001954.01.T01 MTR_4g028660 38.562 306 150 10 84 355 92 393 3.03e-46 162
MsG0180001954.01.T01 MTR_4g027450 38.562 306 150 10 84 355 92 393 3.03e-46 162
MsG0180001954.01.T01 MTR_8g063440 34.768 302 166 8 84 361 89 383 4.57e-46 162
MsG0180001954.01.T01 MTR_6g086825 36.446 332 159 12 74 361 137 460 1.04e-45 162
MsG0180001954.01.T01 MTR_2g034700 36.875 320 164 11 57 354 66 369 1.43e-45 160
MsG0180001954.01.T01 MTR_6g079810 37.349 332 151 12 81 362 140 464 2.97e-45 161
MsG0180001954.01.T01 MTR_7g017040 37.383 321 152 12 81 361 87 398 3.63e-45 159
MsG0180001954.01.T01 MTR_0054s0090 37.963 324 151 12 81 361 90 406 4.02e-45 160
MsG0180001954.01.T01 MTR_5g097350 38.796 299 150 15 78 355 95 381 2.05e-44 157
MsG0180001954.01.T01 MTR_0134s0060 35.821 335 152 14 81 360 85 411 4.95e-44 157
MsG0180001954.01.T01 MTR_7g017130 33.441 311 174 8 81 362 92 398 7.81e-44 156
MsG0180001954.01.T01 MTR_2g038680 37.538 325 145 13 84 360 96 410 7.84e-44 156
MsG0180001954.01.T01 MTR_0129s0070 37.107 318 148 14 83 361 92 396 8.93e-44 156
MsG0180001954.01.T01 MTR_0831s0010 36.184 304 157 10 85 362 130 422 1.20e-43 156
MsG0180001954.01.T01 MTR_6g079770 36.716 335 151 14 81 361 90 417 2.42e-43 155
MsG0180001954.01.T01 MTR_0054s0100 37.346 324 155 12 81 361 90 408 2.65e-43 155
MsG0180001954.01.T01 MTR_5g096630 33.746 323 164 12 84 361 97 414 3.83e-43 154
MsG0180001954.01.T01 MTR_4g007940 36.593 317 141 15 84 354 33 335 4.85e-43 152
MsG0180001954.01.T01 MTR_5g096910 35.831 307 159 11 84 360 94 392 7.28e-43 153
MsG0180001954.01.T01 MTR_7g023800 39.590 293 131 15 81 352 35 302 3.34e-42 149
MsG0180001954.01.T01 MTR_4g102410 37.931 319 144 15 84 359 141 448 3.45e-42 153
MsG0180001954.01.T01 MTR_5g068490 38.434 281 149 10 88 358 8 274 4.02e-42 148
MsG0180001954.01.T01 MTR_7g017170 32.198 323 174 9 81 362 92 410 1.47e-41 150
MsG0180001954.01.T01 MTR_5g059430 36.280 328 155 14 79 361 108 426 2.34e-41 150
MsG0180001954.01.T01 MTR_4g028750 37.049 305 142 13 76 339 81 376 2.65e-41 149
MsG0180001954.01.T01 MTR_2g104340 34.347 329 157 14 81 362 81 397 5.25e-41 149
MsG0180001954.01.T01 MTR_5g097220 34.911 338 153 15 81 361 81 408 1.22e-40 148
MsG0180001954.01.T01 MTR_3g011120 39.446 289 144 13 84 350 90 369 1.66e-40 146
MsG0180001954.01.T01 MTR_5g069390 36.533 323 153 13 79 359 103 415 2.42e-40 149
MsG0180001954.01.T01 MTR_2g104280 35.671 328 153 14 84 362 88 406 6.91e-40 145
MsG0180001954.01.T01 MTR_8g467670 35.667 300 159 11 84 360 90 378 1.84e-39 144
MsG0180001954.01.T01 MTR_0129s0120 34.615 338 171 13 57 356 66 391 2.24e-39 144
MsG0180001954.01.T01 MTR_6g078010 36.721 305 153 14 81 356 37 330 9.69e-39 141
MsG0180001954.01.T01 MTR_5g043605 34.006 347 186 16 40 358 5 336 2.17e-38 141
MsG0180001954.01.T01 MTR_2g066200 34.641 306 167 11 83 360 87 387 2.65e-38 141
MsG0180001954.01.T01 MTR_0129s0050 34.954 329 154 13 84 361 89 408 3.60e-38 141
MsG0180001954.01.T01 MTR_2g065840 36.482 307 160 13 84 360 87 388 3.61e-38 140
MsG0180001954.01.T01 MTR_1g007410 35.484 310 148 13 84 353 101 398 3.81e-38 141
MsG0180001954.01.T01 MTR_5g074800 34.405 311 151 14 84 353 38 336 4.50e-38 139
MsG0180001954.01.T01 MTR_2g021770 34.365 323 157 12 84 361 95 407 9.59e-38 140
MsG0180001954.01.T01 MTR_4g055820 39.179 268 109 12 84 306 92 350 9.91e-38 139
MsG0180001954.01.T01 MTR_6g079790 35.077 325 150 13 80 361 3 309 1.02e-37 137
MsG0180001954.01.T01 MTR_2g065860 36.808 307 162 12 84 362 87 389 1.04e-37 139
MsG0180001954.01.T01 MTR_8g012090 38.519 270 115 15 81 321 82 329 1.53e-37 138
MsG0180001954.01.T01 MTR_6g478230 36.991 319 145 15 84 361 104 407 2.35e-37 139
MsG0180001954.01.T01 MTR_2g066170 35.526 304 163 11 84 360 88 385 4.73e-37 137
MsG0180001954.01.T01 MTR_2g021800 34.713 314 156 11 84 354 102 409 1.37e-36 137
MsG0180001954.01.T01 MTR_5g097330 34.161 322 152 16 81 356 91 398 1.58e-36 137
MsG0180001954.01.T01 MTR_2g022000 34.603 315 151 11 84 352 101 406 2.21e-36 137
MsG0180001954.01.T01 MTR_4g014370 35.366 328 151 15 84 360 91 408 2.49e-36 136
MsG0180001954.01.T01 MTR_6g090180 34.700 317 156 13 84 356 124 433 7.19e-36 135
MsG0180001954.01.T01 MTR_2g066150 34.146 328 181 13 52 361 50 360 9.44e-36 134
MsG0180001954.01.T01 MTR_7g111700 35.144 313 148 14 81 361 76 365 1.68e-35 133
MsG0180001954.01.T01 MTR_2g021950 34.416 308 158 12 84 356 94 392 3.73e-35 133
MsG0180001954.01.T01 MTR_8g041270 34.969 326 155 16 85 362 91 407 4.54e-35 133
MsG0180001954.01.T01 MTR_4g117550 34.628 309 140 13 84 361 96 373 4.59e-35 132
MsG0180001954.01.T01 MTR_7g079640 36.786 280 137 13 84 339 91 354 1.28e-34 131
MsG0180001954.01.T01 MTR_1g007450 33.553 304 153 12 84 347 88 382 5.15e-34 130
MsG0180001954.01.T01 MTR_6g090250 32.081 346 158 14 81 357 83 420 7.53e-34 130
MsG0180001954.01.T01 MTR_8g064890 33.866 313 157 14 84 361 91 388 8.90e-34 129
MsG0180001954.01.T01 MTR_5g064700 33.041 342 154 15 83 361 39 368 2.00e-33 127
MsG0180001954.01.T01 MTR_5g097680 32.370 346 155 15 84 362 95 428 2.92e-33 128
MsG0180001954.01.T01 MTR_1g050312 35.789 285 144 11 84 350 21 284 3.51e-33 125
MsG0180001954.01.T01 MTR_7g069550 34.202 307 155 16 84 353 95 391 7.72e-33 126
MsG0180001954.01.T01 MTR_6g004450 41.885 191 95 6 179 360 85 268 1.05e-32 123
MsG0180001954.01.T01 MTR_1g046950 61.468 109 37 2 1 104 28 136 2.20e-32 119
MsG0180001954.01.T01 MTR_5g092850 33.628 339 160 16 70 356 72 397 2.55e-32 125
MsG0180001954.01.T01 MTR_6g087620 34.122 296 147 15 83 351 5 279 3.21e-32 122
MsG0180001954.01.T01 MTR_2g021920 41.146 192 96 6 178 356 81 268 4.88e-31 119
MsG0180001954.01.T01 MTR_5g008695 68.182 88 25 1 1 85 1 88 1.15e-30 113
MsG0180001954.01.T01 MTR_5g097850 35.273 275 122 9 101 360 11 244 1.32e-30 117
MsG0180001954.01.T01 MTR_5g008705 58.879 107 41 2 1 104 18 124 5.16e-30 112
MsG0180001954.01.T01 MTR_5g079340 63.529 85 31 0 1 85 28 112 7.12e-30 112
MsG0180001954.01.T01 MTR_1g047100 67.816 87 26 1 1 85 28 114 8.11e-30 112
MsG0180001954.01.T01 MTR_1g007430 36.704 267 120 14 84 316 72 323 1.03e-29 117
MsG0180001954.01.T01 MTR_5g074850 33.670 297 147 12 84 336 109 399 1.16e-29 120
MsG0180001954.01.T01 MTR_5g023970 36.649 191 109 6 180 361 9 196 1.74e-29 112
MsG0180001954.01.T01 MTR_5g024260 34.459 296 146 13 84 339 97 384 2.18e-29 117
MsG0180001954.01.T01 MTR_5g092860 33.882 304 135 16 78 336 66 348 1.19e-28 114
MsG0180001954.01.T01 MTR_4g014370 32.313 294 123 11 84 360 102 336 1.26e-28 114
MsG0180001954.01.T01 MTR_7g016930 38.034 234 116 9 83 290 74 304 2.12e-28 113
MsG0180001954.01.T01 MTR_8g039500 37.586 290 144 15 69 330 66 346 4.04e-28 112
MsG0180001954.01.T01 MTR_5g076490 38.384 198 102 6 181 362 144 337 8.30e-28 112
MsG0180001954.01.T01 MTR_8g036970 33.700 273 120 13 84 340 76 303 1.06e-27 112
MsG0180001954.01.T01 MTR_6g461660 32.588 313 168 14 81 356 89 395 1.54e-27 112
MsG0180001954.01.T01 MTR_3g067610 56.471 85 34 1 1 85 29 110 1.75e-27 105
MsG0180001954.01.T01 MTR_7g104710 40.278 216 82 12 84 274 93 286 1.89e-27 110
MsG0180001954.01.T01 MTR_5g086590 34.361 227 124 7 125 334 14 232 3.44e-27 107
MsG0180001954.01.T01 MTR_5g022850 31.173 324 170 15 81 362 42 354 5.61e-27 110
MsG0180001954.01.T01 MTR_6g090260 39.691 194 95 8 180 357 73 260 6.50e-27 107
MsG0180001954.01.T01 MTR_4g016000 37.931 203 90 9 129 309 2 190 6.95e-27 106
MsG0180001954.01.T01 MTR_5g075370 32.890 301 147 13 84 336 82 375 1.05e-26 112
MsG0180001954.01.T01 MTR_8g063350 31.849 292 172 12 84 352 50 337 2.77e-25 105
MsG0180001954.01.T01 MTR_8g064790 30.070 286 155 10 84 361 89 337 3.89e-25 104
MsG0180001954.01.T01 MTR_5g072060 31.186 295 134 12 84 337 82 348 4.53e-25 107
MsG0180001954.01.T01 MTR_8g063330 33.460 263 141 11 84 317 94 351 8.05e-25 105
MsG0180001954.01.T01 MTR_2g064950 30.769 325 138 16 84 352 87 380 1.00e-24 104
MsG0180001954.01.T01 MTR_8g013000 32.292 288 130 13 84 323 89 359 1.64e-24 103
MsG0180001954.01.T01 MTR_3g018750 36.634 202 100 8 176 356 42 236 1.84e-24 100
MsG0180001954.01.T01 MTR_1g050308 29.831 295 133 9 84 361 110 347 8.16e-24 100
MsG0180001954.01.T01 MTR_7g019250 32.584 267 134 10 100 338 165 413 1.52e-23 101
MsG0180001954.01.T01 MTR_8g063370 27.027 296 181 10 84 352 96 383 9.47e-23 98.6
MsG0180001954.01.T01 MTR_5g091140 32.028 281 145 12 84 335 127 390 9.53e-23 99.0
MsG0180001954.01.T01 MTR_5g066420 40.000 135 70 3 222 350 20 149 6.33e-21 88.6
MsG0180001954.01.T01 MTR_5g096900 33.735 249 120 12 70 281 72 312 1.36e-20 91.7
MsG0180001954.01.T01 MTR_6g079730 39.716 141 75 4 222 357 11 146 1.96e-20 87.0
MsG0180001954.01.T01 MTR_3g030620 39.161 143 77 3 224 359 41 180 2.59e-20 87.8
MsG0180001954.01.T01 MTR_5g098950 32.589 224 108 7 115 318 75 275 5.04e-20 89.0
MsG0180001954.01.T01 MTR_1g098180 32.171 258 134 8 126 358 122 363 3.01e-19 88.2
MsG0180001954.01.T01 MTR_5g096920 33.548 155 91 5 212 359 1 150 5.77e-19 83.2
MsG0180001954.01.T01 MTR_5g097660 43.689 103 57 1 255 357 15 116 3.21e-18 80.1
MsG0180001954.01.T01 MTR_5g036250 43.925 107 57 2 255 361 8 111 4.82e-18 81.6
MsG0180001954.01.T01 MTR_5g091150 33.333 207 86 9 91 267 2 186 1.16e-17 80.9
MsG0180001954.01.T01 MTR_2g022020 42.991 107 55 3 251 357 16 116 2.78e-17 77.4
MsG0180001954.01.T01 MTR_6g009350 36.601 153 81 5 179 319 44 192 8.77e-17 78.2
MsG0180001954.01.T01 MTR_5g097540 34.286 175 102 6 181 349 96 263 9.59e-17 80.1
MsG0180001954.01.T01 MTR_8g107110 49.412 85 43 0 1 85 48 132 1.67e-16 76.6
MsG0180001954.01.T01 MTR_7g017150 33.679 193 112 7 181 362 81 268 2.10e-16 78.6
MsG0180001954.01.T01 MTR_7g017120 33.161 193 113 7 181 362 80 267 7.70e-16 77.0
MsG0180001954.01.T01 MTR_5g097820 31.250 208 91 10 60 229 76 269 1.13e-15 76.6
MsG0180001954.01.T01 MTR_5g096050 49.351 77 37 1 225 299 30 106 2.52e-15 72.0
MsG0180001954.01.T01 MTR_6g079570 45.977 87 44 3 1 85 50 135 9.90e-15 72.0
MsG0180001954.01.T01 MTR_1g046960 57.627 59 23 1 1 57 1 59 1.06e-13 67.0
MsG0180001954.01.T01 MTR_5g086780 37.234 94 57 1 243 336 136 227 1.68e-13 69.7
MsG0180001954.01.T01 MTR_8g063310 25.329 304 183 14 84 352 94 388 5.59e-13 70.1
MsG0180001954.01.T01 MTR_4g133170 22.697 304 183 15 72 340 53 339 5.28e-12 67.0
MsG0180001954.01.T01 MTR_3g009240 24.809 262 155 12 75 313 93 335 8.18e-12 66.2
MsG0180001954.01.T01 MTR_1g032070 37.209 86 53 1 1 85 70 155 9.77e-12 63.9
MsG0180001954.01.T01 MTR_0168s0120 33.696 92 59 2 2 92 1 91 1.32e-11 61.2
MsG0180001954.01.T01 MTR_6g025320 33.696 92 59 2 2 92 1 91 1.32e-11 61.2
MsG0180001954.01.T01 MTR_3g030250 23.828 256 158 9 116 355 144 378 1.57e-11 65.5
MsG0180001954.01.T01 MTR_8g101580 25.267 281 170 12 101 355 127 393 1.70e-11 65.5
MsG0180001954.01.T01 MTR_1g050305 48.235 85 37 3 260 339 21 103 2.34e-11 61.6
MsG0180001954.01.T01 MTR_1g097560 26.562 256 165 10 108 355 132 372 3.03e-11 64.7
MsG0180001954.01.T01 MTR_3g010970 27.136 199 106 8 108 297 155 323 3.71e-11 64.3
MsG0180001954.01.T01 MTR_4g067270 45.833 72 36 2 13 82 41 111 8.66e-11 60.1
MsG0180001954.01.T01 MTR_3g009310 24.609 256 150 12 79 315 109 340 9.27e-11 63.2
MsG0180001954.01.T01 MTR_3g089090 36.905 84 53 0 2 85 30 113 9.66e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001954.01.T01 AT1G18210 41.935 93 47 3 4 96 50 135 2.03e-14 70.9
MsG0180001954.01.T01 AT1G18210 41.935 93 47 3 4 96 50 135 2.03e-14 70.9
MsG0180001954.01.T01 AT5G17470 40.506 79 44 1 10 85 35 113 2.92e-13 67.0
MsG0180001954.01.T01 AT2G36180 43.590 78 40 1 10 83 33 110 1.53e-12 65.1
MsG0180001954.01.T01 AT1G73630 38.889 90 48 3 4 93 47 129 2.56e-12 64.7
MsG0180001954.01.T01 AT1G66410 34.783 92 58 2 2 92 1 91 3.58e-12 63.2
MsG0180001954.01.T01 AT1G66410 34.783 92 58 2 2 92 1 91 3.58e-12 63.2
MsG0180001954.01.T01 AT3G03400 40.000 75 42 1 12 83 40 114 9.83e-12 62.4
MsG0180001954.01.T01 AT1G66400 36.047 86 52 1 1 83 39 124 1.28e-11 62.8
MsG0180001954.01.T01 AT3G43810 33.696 92 59 2 2 92 1 91 1.38e-11 61.2
MsG0180001954.01.T01 AT3G43810 33.696 92 59 2 2 92 1 91 1.38e-11 61.2
MsG0180001954.01.T01 AT1G24620 37.931 87 51 2 1 85 61 146 2.25e-11 62.8
MsG0180001954.01.T01 AT5G37780 34.409 93 59 2 1 92 23 114 4.48e-11 60.5

Find 85 sgRNAs with CRISPR-Local

Find 262 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACATAGTTCTTAATAAAAT+TGG 0.142946 1:-29838258 MsG0180001954.01.T01:CDS
AAGGAAAATCTTCTTCCTTT+TGG 0.192708 1:-29834322 MsG0180001954.01.T01:CDS
TTCGGTTCGGTTAGTGTTAA+TGG 0.218248 1:-29836140 MsG0180001954.01.T01:CDS
AGTTGCTTGGTGATGTTAAA+TGG 0.250919 1:-29834616 MsG0180001954.01.T01:CDS
CGTCTTTCCTAAAGAATCTA+TGG 0.284132 1:+29834443 None:intergenic
AAAATCCATATGTGAAAATA+AGG 0.285202 1:+29834559 None:intergenic
TTCACATTAGTCTCCCAAAA+AGG 0.302674 1:+29834904 None:intergenic
CTTTGTCTCTGCAAAGACTA+TGG 0.306363 1:-29836113 MsG0180001954.01.T01:CDS
CTAATGTGAATGACCATTAT+TGG 0.308616 1:-29834891 MsG0180001954.01.T01:CDS
CACAGAAAGGGTCATACTTC+TGG 0.331017 1:+29834848 None:intergenic
ATAAGAGCCATAGATTCTTT+AGG 0.340887 1:-29834450 MsG0180001954.01.T01:CDS
TGAGTTGAACATTGACTATA+AGG 0.341469 1:-29836009 MsG0180001954.01.T01:intron
AATGACATCATAACATTTCC+TGG 0.342007 1:+29834712 None:intergenic
GATTGCAGAAATTGAGGTTA+AGG 0.351081 1:-29834377 MsG0180001954.01.T01:CDS
GGACTCATAGCATAACTTAA+TGG 0.354046 1:+29834487 None:intergenic
AGAACTATGTGCAATTCTTT+CGG 0.363167 1:+29838270 None:intergenic
GGGTTGATATGCCTATTTCT+TGG 0.391475 1:-29834783 MsG0180001954.01.T01:CDS
GTCCTACCTGGCCAAGAAAT+AGG 0.395770 1:+29834772 None:intergenic
GACTTAAAGTCCCTGGTAGC+AGG 0.410289 1:+29836062 None:intergenic
ATTGCAGAAATTGAGGTTAA+GGG 0.414219 1:-29834376 MsG0180001954.01.T01:CDS
GGAAGAATGCTTTCAACACT+TGG 0.420989 1:-29838474 None:intergenic
CAGGTGAAAGGTTTGAGGAC+AGG 0.430604 1:-29836202 MsG0180001954.01.T01:CDS
TTCAACTCAATGTTCTCAAA+TGG 0.431749 1:+29836023 None:intergenic
CTCAATGTTCTCAAATGGTT+GGG 0.441410 1:+29836028 None:intergenic
TAATGTGAATGACCATTATT+GGG 0.453429 1:-29834890 MsG0180001954.01.T01:CDS
ACTCAATGTTCTCAAATGGT+TGG 0.460685 1:+29836027 None:intergenic
AACTCTTGGAGGAAACTTGA+AGG 0.465371 1:-29834805 MsG0180001954.01.T01:CDS
TCAAAGGATTGCAGAAATTG+AGG 0.478612 1:-29834383 MsG0180001954.01.T01:CDS
TGACCATTATTGGGAGTTGA+AGG 0.478648 1:-29834881 MsG0180001954.01.T01:CDS
CCTCCTTCAACTCCCAATAA+TGG 0.479010 1:+29834878 None:intergenic
TTCTCAAATGGTTGGGTAGG+AGG 0.481077 1:+29836035 None:intergenic
AGGACTCATGTGAACTTGAA+TGG 0.481172 1:-29834754 MsG0180001954.01.T01:CDS
ATAGCCTCAGAAGTAACTCT+TGG 0.482779 1:-29834819 MsG0180001954.01.T01:CDS
TCTTTCAGGTGAAAGGTTTG+AGG 0.483208 1:-29836207 MsG0180001954.01.T01:intron
TTTATTACTTCATCAAATGT+TGG 0.486517 1:+29838444 None:intergenic
GCCTCAGAAGTAACTCTTGG+AGG 0.494079 1:-29834816 MsG0180001954.01.T01:CDS
AGGTGAAAGGTTTGAGGACA+GGG 0.496008 1:-29836201 MsG0180001954.01.T01:CDS
TATTGTGGAACCCTGCTACC+AGG 0.496790 1:-29836073 MsG0180001954.01.T01:CDS
AGTGCATTCTTTCAGGTGAA+AGG 0.498126 1:-29836214 MsG0180001954.01.T01:intron
AGGACAGGGTTAGATTAGAC+TGG 0.509396 1:-29836187 MsG0180001954.01.T01:CDS
ATTGTGGAACCCTGCTACCA+GGG 0.509463 1:-29836072 MsG0180001954.01.T01:CDS
AAATGGTTGGGTAGGAGGAA+TGG 0.509592 1:+29836040 None:intergenic
AACCCGGTGTCAAGGAATCA+TGG 0.510712 1:-29834531 MsG0180001954.01.T01:CDS
ACTCAGGTGTAATACTTGAT+TGG 0.511634 1:-29834645 MsG0180001954.01.T01:CDS
AACGGATGTTGTAATTTACA+AGG 0.511737 1:-29834341 MsG0180001954.01.T01:CDS
GTTGCTTGGTGATGTTAAAT+GGG 0.519218 1:-29834615 MsG0180001954.01.T01:CDS
TTAACATCACCAAGCAACTC+TGG 0.519755 1:+29834620 None:intergenic
TTTACACAACCACTGTATTG+TGG 0.520861 1:-29836088 MsG0180001954.01.T01:CDS
TGATGAAGTAATAAAGATGA+TGG 0.523203 1:-29838436 MsG0180001954.01.T01:CDS
GGCTATATATCTCACAGAAA+GGG 0.524232 1:+29834836 None:intergenic
ATGGACTTACCCTCTGACTC+AGG 0.524458 1:-29834661 MsG0180001954.01.T01:CDS
CGTCCTTGTCGACGTCGTAA+AGG 0.532322 1:+29838304 None:intergenic
TAACGGCTCATTCCTCCAAA+AGG 0.532652 1:+29834307 None:intergenic
AGTCCATGATTCCTTGACAC+CGG 0.542807 1:+29834528 None:intergenic
AGGCTATATATCTCACAGAA+AGG 0.550853 1:+29834835 None:intergenic
TTCTTTAGGAAAGACGAAGA+AGG 0.551485 1:-29834436 MsG0180001954.01.T01:CDS
TTTGGGTCAACCCGGTGTCA+AGG 0.551674 1:-29834539 MsG0180001954.01.T01:CDS
GATATGCCTATTTCTTGGCC+AGG 0.557406 1:-29834778 MsG0180001954.01.T01:CDS
AGGAATGGACTTAAAGTCCC+TGG 0.559384 1:+29836055 None:intergenic
GTCCATGATTCCTTGACACC+GGG 0.561844 1:+29834529 None:intergenic
ACTCTTGGAGGAAACTTGAA+GGG 0.562682 1:-29834804 MsG0180001954.01.T01:CDS
GATATTGATCAGAACGGCGA+CGG 0.563636 1:-29838411 MsG0180001954.01.T01:CDS
CTCTTATTCACTGTTCCGAT+AGG 0.564259 1:+29834466 None:intergenic
ATGTTCTCAAATGGTTGGGT+AGG 0.565331 1:+29836032 None:intergenic
AGTTATGCTATGAGTCCTAT+CGG 0.570786 1:-29834481 MsG0180001954.01.T01:CDS
TGATTGGTGCCAGAGTTGCT+TGG 0.571098 1:-29834629 MsG0180001954.01.T01:CDS
TTACAACATCCGTTGCAAGC+AGG 0.572541 1:+29834350 None:intergenic
CGACCTTTACGACGTCGACA+AGG 0.572934 1:-29838307 MsG0180001954.01.T01:CDS
AAGTATTACACCTGAGTCAG+AGG 0.577228 1:+29834651 None:intergenic
TTTGCCGATGTCTACTGCAC+CGG 0.581636 1:-29838369 MsG0180001954.01.T01:CDS
ATGACATCATAACATTTCCT+GGG 0.597763 1:+29834713 None:intergenic
AAGTTCACATGAGTCCTACC+TGG 0.601231 1:+29834760 None:intergenic
CCATTATTGGGAGTTGAAGG+AGG 0.602464 1:-29834878 MsG0180001954.01.T01:CDS
ACTTAAAGTCCCTGGTAGCA+GGG 0.609668 1:+29836063 None:intergenic
TAAGGGAAACCTGCTTGCAA+CGG 0.615831 1:-29834359 MsG0180001954.01.T01:CDS
CTCTTGGAGGAAACTTGAAG+GGG 0.616599 1:-29834803 MsG0180001954.01.T01:CDS
ATTGGTGAGAAATACTCCCT+CGG 0.618828 1:-29838240 MsG0180001954.01.T01:intron
TCCTCCAAGAGTTACTTCTG+AGG 0.634400 1:+29834815 None:intergenic
TGCCTATTTCTTGGCCAGGT+AGG 0.635063 1:-29834774 MsG0180001954.01.T01:CDS
ATGGAAGATATTGATCAGAA+CGG 0.635154 1:-29838417 MsG0180001954.01.T01:CDS
CTTTACGACGTCGACAAGGA+CGG 0.635439 1:-29838303 MsG0180001954.01.T01:CDS
AGCACCGGTGCAGTAGACAT+CGG 0.635909 1:+29838365 None:intergenic
GCTTTGATATTAGAACTCAA+AGG 0.646079 1:-29834399 MsG0180001954.01.T01:CDS
AGTATTACACCTGAGTCAGA+GGG 0.668148 1:+29834652 None:intergenic
AGCAGGGTTCCACAATACAG+TGG 0.710592 1:+29836079 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAAATAAAAAGGAAA+AGG + Chr1:29834673-29834692 None:intergenic 10.0%
!!! AATTAATAATTACTTTTAGA+TGG + Chr1:29837216-29837235 None:intergenic 10.0%
!! AAATTGAAAAATCAAAGAAA+AGG - Chr1:29835402-29835421 MsG0180001954.01.T01:intron 15.0%
!! AACAAATATTAGATGAAATT+TGG + Chr1:29837065-29837084 None:intergenic 15.0%
!! GTATGTATAACATATTATTA+AGG - Chr1:29835823-29835842 MsG0180001954.01.T01:intron 15.0%
!! TATAACATATTATTAAGGTT+TGG - Chr1:29835828-29835847 MsG0180001954.01.T01:intron 15.0%
!! TATATATATTAAGTTTATGC+TGG - Chr1:29835510-29835529 MsG0180001954.01.T01:intron 15.0%
!!! TTATTTTGAAATTGTTGTTA+AGG - Chr1:29834686-29834705 MsG0180001954.01.T01:CDS 15.0%
!! AAAATCCATATGTGAAAATA+AGG + Chr1:29838218-29838237 None:intergenic 20.0%
!! ATAATGAATACATTGATGTT+TGG - Chr1:29835281-29835300 MsG0180001954.01.T01:intron 20.0%
!! ATGAAATTTGGAGAAAAATT+GGG + Chr1:29837053-29837072 None:intergenic 20.0%
!! ATTATATCTGCATTAATGAA+TGG - Chr1:29834985-29835004 MsG0180001954.01.T01:intron 20.0%
!! CACATAGTTCTTAATAAAAT+TGG - Chr1:29834516-29834535 MsG0180001954.01.T01:CDS 20.0%
!! GAAAATTCAGTAATTTAAAC+AGG + Chr1:29837602-29837621 None:intergenic 20.0%
!! TAATCAACTACAATAATGAA+TGG - Chr1:29835079-29835098 MsG0180001954.01.T01:intron 20.0%
!! TTCAAAATTTGTCTATAAGA+TGG + Chr1:29835315-29835334 None:intergenic 20.0%
!! TTTATTACTTCATCAAATGT+TGG + Chr1:29834333-29834352 None:intergenic 20.0%
!!! ATATTATATGATTTAAAGCC+TGG - Chr1:29835547-29835566 MsG0180001954.01.T01:intron 20.0%
!!! ATTTCTTTAAAGAGAGAATA+TGG + Chr1:29835035-29835054 None:intergenic 20.0%
!!! TATGTTTGATTTAAGGATTT+AGG - Chr1:29834761-29834780 MsG0180001954.01.T01:CDS 20.0%
!!! TATTTTCACATATGGATTTT+GGG - Chr1:29838218-29838237 MsG0180001954.01.T01:intron 20.0%
!!! TCTGTATTATGTTTGATTTA+AGG - Chr1:29834754-29834773 MsG0180001954.01.T01:CDS 20.0%
!!! TTATTTTCACATATGGATTT+TGG - Chr1:29838217-29838236 MsG0180001954.01.T01:intron 20.0%
!!! TTTATCAAGGTTCTAAATAA+CGG - Chr1:29836800-29836819 MsG0180001954.01.T01:intron 20.0%
!!! TTTTTTTGTTTGAGTGAATA+AGG + Chr1:29837321-29837340 None:intergenic 20.0%
!!! TTTTTTTTTCATAAACAACC+AGG + Chr1:29835991-29836010 None:intergenic 20.0%
! AAAGAAAAAGTTGCAACTTT+AGG - Chr1:29836121-29836140 MsG0180001954.01.T01:CDS 25.0%
! AAGCAAAATGAAAACCTATT+AGG + Chr1:29836035-29836054 None:intergenic 25.0%
! AATGCATCAGAATATAAACA+AGG + Chr1:29836214-29836233 None:intergenic 25.0%
! AATTACTTACTTCTCTTCTT+TGG + Chr1:29837506-29837525 None:intergenic 25.0%
! ACCTAAATACTCAAATTAAG+TGG - Chr1:29837085-29837104 MsG0180001954.01.T01:intron 25.0%
! AGTTTATGCTGGAAATTATT+TGG - Chr1:29835576-29835595 MsG0180001954.01.T01:intron 25.0%
! ATACATGACACAATTTACTT+AGG + Chr1:29834934-29834953 None:intergenic 25.0%
! ATATTTGAAGAGAAAACTTC+TGG + Chr1:29834812-29834831 None:intergenic 25.0%
! GAAACATGTTCATGAAATAA+GGG + Chr1:29836475-29836494 None:intergenic 25.0%
! GATGAAATTTGGAGAAAAAT+TGG + Chr1:29837054-29837073 None:intergenic 25.0%
! GTTTATGCTGGAAATTATTT+GGG - Chr1:29835577-29835596 MsG0180001954.01.T01:intron 25.0%
! TAAACAAGGACTGATATAAA+TGG + Chr1:29836200-29836219 None:intergenic 25.0%
! TAATGTGAATGACCATTATT+GGG - Chr1:29837884-29837903 MsG0180001954.01.T01:intron 25.0%
! TGAATCTTATCTTGAAAATC+AGG + Chr1:29837566-29837585 None:intergenic 25.0%
! TGATGAAGTAATAAAGATGA+TGG - Chr1:29834338-29834357 MsG0180001954.01.T01:CDS 25.0%
!! AAATATTCTCTGTTCTCTTT+TGG - Chr1:29834834-29834853 MsG0180001954.01.T01:CDS 25.0%
!! ACCACTTAATTTGAGTATTT+AGG + Chr1:29837089-29837108 None:intergenic 25.0%
!! ATTTTGCTTGTTCAAAGAAT+AGG - Chr1:29836046-29836065 MsG0180001954.01.T01:CDS 25.0%
!! CATGAGATTGCAAATTATTT+TGG - Chr1:29835170-29835189 MsG0180001954.01.T01:intron 25.0%
!! GCTTTGATTTAGATGAAATT+AGG - Chr1:29835732-29835751 MsG0180001954.01.T01:intron 25.0%
!!! AACACCAAGGTTTTAAAAAA+CGG + Chr1:29837796-29837815 None:intergenic 25.0%
!!! ACTTGAATGGATTTTTCTAT+TGG - Chr1:29838033-29838052 MsG0180001954.01.T01:intron 25.0%
!!! CAAAGAGATATTTTTTGCAA+TGG - Chr1:29838094-29838113 MsG0180001954.01.T01:intron 25.0%
!!! TTTTTTTAGGATTTTCGGTT+CGG - Chr1:29836621-29836640 MsG0180001954.01.T01:intron 25.0%
AAATTAGGTACGGATGAAAT+TGG - Chr1:29835665-29835684 MsG0180001954.01.T01:intron 30.0%
AACGGATGTTGTAATTTACA+AGG - Chr1:29838433-29838452 MsG0180001954.01.T01:CDS 30.0%
AATCGTGTGAGAGATTTAAT+AGG + Chr1:29836414-29836433 None:intergenic 30.0%
AATGAATGGAAAAAGCTTTG+TGG - Chr1:29835093-29835112 MsG0180001954.01.T01:intron 30.0%
AATGACATCATAACATTTCC+TGG + Chr1:29838065-29838084 None:intergenic 30.0%
AATGACGGTGATGAAAAATT+AGG - Chr1:29835650-29835669 MsG0180001954.01.T01:intron 30.0%
AGAACTATGTGCAATTCTTT+CGG + Chr1:29834507-29834526 None:intergenic 30.0%
ATAAGAGCCATAGATTCTTT+AGG - Chr1:29838324-29838343 MsG0180001954.01.T01:CDS 30.0%
ATCATCATGATCGTCAAATT+TGG + Chr1:29836513-29836532 None:intergenic 30.0%
ATGACACAATTTACTTAGGA+GGG + Chr1:29834930-29834949 None:intergenic 30.0%
ATGACATCATAACATTTCCT+GGG + Chr1:29838064-29838083 None:intergenic 30.0%
ATGGAAGATATTGATCAGAA+CGG - Chr1:29834357-29834376 MsG0180001954.01.T01:CDS 30.0%
ATTGCAGAAATTGAGGTTAA+GGG - Chr1:29838398-29838417 MsG0180001954.01.T01:CDS 30.0%
CCATATACTGTCAATTCTTT+CGG - Chr1:29836253-29836272 MsG0180001954.01.T01:intron 30.0%
CGAAACATGTTCATGAAATA+AGG + Chr1:29836476-29836495 None:intergenic 30.0%
CTAATGTGAATGACCATTAT+TGG - Chr1:29837883-29837902 MsG0180001954.01.T01:intron 30.0%
GAACCGAAAATCCTAAAAAA+AGG + Chr1:29836622-29836641 None:intergenic 30.0%
GCTTTGATATTAGAACTCAA+AGG - Chr1:29838375-29838394 MsG0180001954.01.T01:CDS 30.0%
GTAATTTAAACAGGAGAAGA+AGG + Chr1:29837593-29837612 None:intergenic 30.0%
TATACAAATAACGGTCTTTG+CGG - Chr1:29836883-29836902 MsG0180001954.01.T01:intron 30.0%
TGAGAAAAGCTAATCCTAAT+AGG - Chr1:29836018-29836037 MsG0180001954.01.T01:CDS 30.0%
TGAGATTTCAATTCATGTCA+TGG - Chr1:29836310-29836329 MsG0180001954.01.T01:intron 30.0%
TTCAACTCAATGTTCTCAAA+TGG + Chr1:29836754-29836773 None:intergenic 30.0%
! AAGGAAAATCTTCTTCCTTT+TGG - Chr1:29838452-29838471 MsG0180001954.01.T01:CDS 30.0%
! CTAATGATTACCGATTGATA+TGG - Chr1:29835627-29835646 MsG0180001954.01.T01:intron 30.0%
! CTTTTTCTTTGCACTTTGAA+AGG + Chr1:29834962-29834981 None:intergenic 30.0%
! TGAGTTGAACATTGACTATA+AGG - Chr1:29836765-29836784 MsG0180001954.01.T01:intron 30.0%
! TGTTCATGAAATAAGGGTTT+TGG + Chr1:29836469-29836488 None:intergenic 30.0%
! TGTTGCTACTTCAAAGAATA+AGG - Chr1:29836334-29836353 MsG0180001954.01.T01:intron 30.0%
! TTTTTGTTCTCAACGTGAAA+AGG - Chr1:29837708-29837727 MsG0180001954.01.T01:intron 30.0%
!! ATTAGGCACTTGTTTTCATT+TGG - Chr1:29835749-29835768 MsG0180001954.01.T01:intron 30.0%
!! GAATAAGGTTTGTTATCACT+TGG + Chr1:29837306-29837325 None:intergenic 30.0%
!! GTGATTTCTAGTGTTTATCA+AGG - Chr1:29836787-29836806 MsG0180001954.01.T01:intron 30.0%
!!! AGACACCTTATTTTCACATA+TGG - Chr1:29838210-29838229 MsG0180001954.01.T01:intron 30.0%
!!! CGTCCTTTTTTTAGGATTTT+CGG - Chr1:29836616-29836635 MsG0180001954.01.T01:intron 30.0%
!!! CTGAATTTTCAAGTCTGTAA+CGG - Chr1:29837612-29837631 MsG0180001954.01.T01:intron 30.0%
!!! GCATTTGCTTTTTGTTAATG+TGG - Chr1:29835424-29835443 MsG0180001954.01.T01:intron 30.0%
AACGTGAAAAGGACCAAAAA+CGG - Chr1:29837719-29837738 MsG0180001954.01.T01:intron 35.0%
ACTCAATGTTCTCAAATGGT+TGG + Chr1:29836750-29836769 None:intergenic 35.0%
ACTCAGGTGTAATACTTGAT+TGG - Chr1:29838129-29838148 MsG0180001954.01.T01:intron 35.0%
AGGCTATATATCTCACAGAA+AGG + Chr1:29837942-29837961 None:intergenic 35.0%
AGTTATGCTATGAGTCCTAT+CGG - Chr1:29838293-29838312 MsG0180001954.01.T01:CDS 35.0%
ATCCTAAAAAAAGGACGAGA+AGG + Chr1:29836613-29836632 None:intergenic 35.0%
CAATCTCATGAAAGATCCTA+CGG + Chr1:29835160-29835179 None:intergenic 35.0%
CATGACACAATTTACTTAGG+AGG + Chr1:29834931-29834950 None:intergenic 35.0%
CCGAAAGAATTGACAGTATA+TGG + Chr1:29836256-29836275 None:intergenic 35.0%
CGGTGATGAAAAATTAGGTA+CGG - Chr1:29835655-29835674 MsG0180001954.01.T01:intron 35.0%
CGTCTTTCCTAAAGAATCTA+TGG + Chr1:29838334-29838353 None:intergenic 35.0%
CTCAATGTTCTCAAATGGTT+GGG + Chr1:29836749-29836768 None:intergenic 35.0%
GATTGCAGAAATTGAGGTTA+AGG - Chr1:29838397-29838416 MsG0180001954.01.T01:CDS 35.0%
GGACTCATAGCATAACTTAA+TGG + Chr1:29838290-29838309 None:intergenic 35.0%
GGCTATATATCTCACAGAAA+GGG + Chr1:29837941-29837960 None:intergenic 35.0%
TCAAAGGATTGCAGAAATTG+AGG - Chr1:29838391-29838410 MsG0180001954.01.T01:CDS 35.0%
TGTGGTATATTGCTTTGATG+TGG - Chr1:29835442-29835461 MsG0180001954.01.T01:intron 35.0%
TTACTAGTTCATGTGTTACC+TGG - Chr1:29835970-29835989 MsG0180001954.01.T01:intron 35.0%
TTCACATGTGCTCAAAATCA+TGG - Chr1:29836432-29836451 MsG0180001954.01.T01:intron 35.0%
TTTACACAACCACTGTATTG+TGG - Chr1:29836686-29836705 MsG0180001954.01.T01:intron 35.0%
! AACGAATTAATAAGTGCCGT+AGG - Chr1:29835141-29835160 MsG0180001954.01.T01:intron 35.0%
! GAAAATCTTCTTCCTTTTGG+AGG - Chr1:29838455-29838474 MsG0180001954.01.T01:CDS 35.0%
! GTAAGTCTTATGACTGTCAA+CGG - Chr1:29835792-29835811 MsG0180001954.01.T01:intron 35.0%
! GTGCATTCGTCTCTTATTTA+AGG + Chr1:29837145-29837164 None:intergenic 35.0%
! TACCGATTGATATGGAATGA+CGG - Chr1:29835635-29835654 MsG0180001954.01.T01:intron 35.0%
! TCACATTAGTCTCCCAAAAA+GGG + Chr1:29837872-29837891 None:intergenic 35.0%
! TCGTCTCTTATTTAAGGTCA+AGG + Chr1:29837139-29837158 None:intergenic 35.0%
! TGGACTAAAGTTTTCGTTGT+TGG - Chr1:29838263-29838282 MsG0180001954.01.T01:CDS 35.0%
! TTCACATTAGTCTCCCAAAA+AGG + Chr1:29837873-29837892 None:intergenic 35.0%
! TTCTTTAGGAAAGACGAAGA+AGG - Chr1:29838338-29838357 MsG0180001954.01.T01:CDS 35.0%
!! AGTTGCTTGGTGATGTTAAA+TGG - Chr1:29838158-29838177 MsG0180001954.01.T01:intron 35.0%
!! CTTGTTTTCATTTGGATGCA+CGG - Chr1:29835757-29835776 MsG0180001954.01.T01:intron 35.0%
!! GTTGCTTGGTGATGTTAAAT+GGG - Chr1:29838159-29838178 MsG0180001954.01.T01:intron 35.0%
!!! CAAGGTTTTAAAAAACGGAC+CGG + Chr1:29837791-29837810 None:intergenic 35.0%
!!! TTTTTCTATTGGTTTAGCCC+AGG - Chr1:29838044-29838063 MsG0180001954.01.T01:intron 35.0%
AAAAGGACCAAAAACGGTCA+CGG - Chr1:29837725-29837744 MsG0180001954.01.T01:intron 40.0%
AACTCTTGGAGGAAACTTGA+AGG - Chr1:29837969-29837988 MsG0180001954.01.T01:intron 40.0%
AAGCAGAACGAAGCAACAAA+CGG - Chr1:29837368-29837387 MsG0180001954.01.T01:intron 40.0%
AAGTATTACACCTGAGTCAG+AGG + Chr1:29838126-29838145 None:intergenic 40.0%
AAGTTGTGCATACGGTGTAT+TGG - Chr1:29837277-29837296 MsG0180001954.01.T01:intron 40.0%
ACTCTTGGAGGAAACTTGAA+GGG - Chr1:29837970-29837989 MsG0180001954.01.T01:intron 40.0%
AGGACTCATGTGAACTTGAA+TGG - Chr1:29838020-29838039 MsG0180001954.01.T01:intron 40.0%
AGTATTACACCTGAGTCAGA+GGG + Chr1:29838125-29838144 None:intergenic 40.0%
ATAGCCTCAGAAGTAACTCT+TGG - Chr1:29837955-29837974 MsG0180001954.01.T01:intron 40.0%
ATGTTCTCAAATGGTTGGGT+AGG + Chr1:29836745-29836764 None:intergenic 40.0%
ATTGGTGAGAAATACTCCCT+CGG - Chr1:29834534-29834553 MsG0180001954.01.T01:CDS 40.0%
CAACTAACGTCAAAGTGTAC+GGG + Chr1:29837825-29837844 None:intergenic 40.0%
CACCGTCATTCCATATCAAT+CGG + Chr1:29835640-29835659 None:intergenic 40.0%
CCAACTAACGTCAAAGTGTA+CGG + Chr1:29837826-29837845 None:intergenic 40.0%
CGTACACTTTGACGTTAGTT+GGG - Chr1:29837824-29837843 MsG0180001954.01.T01:intron 40.0%
CTCTTATTCACTGTTCCGAT+AGG + Chr1:29838311-29838330 None:intergenic 40.0%
CTGATGCATTGCGTGTAATT+TGG - Chr1:29836224-29836243 MsG0180001954.01.T01:intron 40.0%
CTTTGTCTCTGCAAAGACTA+TGG - Chr1:29836661-29836680 MsG0180001954.01.T01:intron 40.0%
GAAGAGTGAAGTTGTGCATA+CGG - Chr1:29837269-29837288 MsG0180001954.01.T01:intron 40.0%
GCAGAACAAGAAGCAACAAA+CGG - Chr1:29837394-29837413 MsG0180001954.01.T01:intron 40.0%
GGAAACTAGTGCATTCTTTC+AGG - Chr1:29836553-29836572 MsG0180001954.01.T01:intron 40.0%
GGACGCTGCTATACAAATAA+CGG - Chr1:29836874-29836893 MsG0180001954.01.T01:intron 40.0%
GTACGGGATAATAAACACCA+AGG + Chr1:29837809-29837828 None:intergenic 40.0%
TGACCATTATTGGGAGTTGA+AGG - Chr1:29837893-29837912 MsG0180001954.01.T01:intron 40.0%
TTAACATCACCAAGCAACTC+TGG + Chr1:29838157-29838176 None:intergenic 40.0%
TTAGTTGGGTTTATGTGCCT+GGG - Chr1:29837838-29837857 MsG0180001954.01.T01:intron 40.0%
TTCTTGGGTCAGAAACAACT+GGG + Chr1:29836151-29836170 None:intergenic 40.0%
TTGGATGCACGGTTAAGATT+TGG - Chr1:29835768-29835787 MsG0180001954.01.T01:intron 40.0%
TTGTTTGCGATTTGCGATTC+TGG + Chr1:29837425-29837444 None:intergenic 40.0%
TTTAAAAAACGGACCGGTAC+CGG + Chr1:29837785-29837804 None:intergenic 40.0%
! AGTGCATTCTTTCAGGTGAA+AGG - Chr1:29836560-29836579 MsG0180001954.01.T01:intron 40.0%
! GAAATTGGTTTAACTCGTCG+CGG - Chr1:29835680-29835699 MsG0180001954.01.T01:intron 40.0%
! TCTTTCAGGTGAAAGGTTTG+AGG - Chr1:29836567-29836586 MsG0180001954.01.T01:intron 40.0%
! TTACTTCTCTTCTTTGGTGC+TGG + Chr1:29837500-29837519 None:intergenic 40.0%
! TTTGTTCAGGTTTGCGATTC+TGG + Chr1:29837350-29837369 None:intergenic 40.0%
!! AAGGTTCTAAATAACGGTCG+CGG - Chr1:29836806-29836825 MsG0180001954.01.T01:intron 40.0%
!! ATATGGATTTTGGGTCAACC+CGG - Chr1:29838227-29838246 MsG0180001954.01.T01:intron 40.0%
!! GGGTTGATATGCCTATTTCT+TGG - Chr1:29837991-29838010 MsG0180001954.01.T01:intron 40.0%
!! TTCGGTTCGGTTAGTGTTAA+TGG - Chr1:29836634-29836653 MsG0180001954.01.T01:intron 40.0%
!!! CGGTCCGTTTTTTAAAACCT+TGG - Chr1:29837789-29837808 MsG0180001954.01.T01:intron 40.0%
AAATGGTTGGGTAGGAGGAA+TGG + Chr1:29836737-29836756 None:intergenic 45.0%
AAGTTCACATGAGTCCTACC+TGG + Chr1:29838017-29838036 None:intergenic 45.0%
ACTTAAAGTCCCTGGTAGCA+GGG + Chr1:29836714-29836733 None:intergenic 45.0%
AGGACAGGGTTAGATTAGAC+TGG - Chr1:29836587-29836606 MsG0180001954.01.T01:intron 45.0%
AGGTGAAAGGTTTGAGGACA+GGG - Chr1:29836573-29836592 MsG0180001954.01.T01:intron 45.0%
AGTCCATGATTCCTTGACAC+CGG + Chr1:29838249-29838268 None:intergenic 45.0%
ATCACAGCAAAATCGCCGTA+CGG + Chr1:29837689-29837708 None:intergenic 45.0%
CACAGAAAGGGTCATACTTC+TGG + Chr1:29837929-29837948 None:intergenic 45.0%
CCAGCATAAACTCATAGACC+AGG + Chr1:29835568-29835587 None:intergenic 45.0%
CCATTATTGGGAGTTGAAGG+AGG - Chr1:29837896-29837915 MsG0180001954.01.T01:intron 45.0%
CCGTACACTTTGACGTTAGT+TGG - Chr1:29837823-29837842 MsG0180001954.01.T01:intron 45.0%
CCTCCTTCAACTCCCAATAA+TGG + Chr1:29837899-29837918 None:intergenic 45.0%
CCTGGTCTATGAGTTTATGC+TGG - Chr1:29835565-29835584 MsG0180001954.01.T01:intron 45.0%
CGAGGAGTTCAAGAAGATGA+TGG - Chr1:29834612-29834631 MsG0180001954.01.T01:CDS 45.0%
CGTTAAGGTTGTCGCGATTT+CGG - Chr1:29836844-29836863 MsG0180001954.01.T01:intron 45.0%
CTCTTGGAGGAAACTTGAAG+GGG - Chr1:29837971-29837990 MsG0180001954.01.T01:intron 45.0%
CTTCTTGGGTCAGAAACAAC+TGG + Chr1:29836152-29836171 None:intergenic 45.0%
GATATTGATCAGAACGGCGA+CGG - Chr1:29834363-29834382 MsG0180001954.01.T01:CDS 45.0%
GTTAGTTGGGTTTATGTGCC+TGG - Chr1:29837837-29837856 MsG0180001954.01.T01:intron 45.0%
TAACGGCTCATTCCTCCAAA+AGG + Chr1:29838470-29838489 None:intergenic 45.0%
TAAGGGAAACCTGCTTGCAA+CGG - Chr1:29838415-29838434 MsG0180001954.01.T01:CDS 45.0%
TCCTCCAAGAGTTACTTCTG+AGG + Chr1:29837962-29837981 None:intergenic 45.0%
TGATGCGATGTTGATGCTGA+TGG - Chr1:29834571-29834590 MsG0180001954.01.T01:CDS 45.0%
TGCTGCAACCGAAAAAGATG+AGG - Chr1:29834428-29834447 MsG0180001954.01.T01:CDS 45.0%
TTAAAAAACGGACCGGTACC+GGG + Chr1:29837784-29837803 None:intergenic 45.0%
TTACAACATCCGTTGCAAGC+AGG + Chr1:29838427-29838446 None:intergenic 45.0%
TTCTCAAATGGTTGGGTAGG+AGG + Chr1:29836742-29836761 None:intergenic 45.0%
TTGGGAAAAGAGTTCACGCA+GGG - Chr1:29835595-29835614 MsG0180001954.01.T01:intron 45.0%
TTTGGGAAAAGAGTTCACGC+AGG - Chr1:29835594-29835613 MsG0180001954.01.T01:intron 45.0%
! AGGAATGGACTTAAAGTCCC+TGG + Chr1:29836722-29836741 None:intergenic 45.0%
! ATGTTGATGCTGATGGTGAC+GGG - Chr1:29834578-29834597 MsG0180001954.01.T01:CDS 45.0%
! CACTAGTTTCCTTTACGACC+AGG + Chr1:29836544-29836563 None:intergenic 45.0%
! GATATGCCTATTTCTTGGCC+AGG - Chr1:29837996-29838015 MsG0180001954.01.T01:intron 45.0%
! GATGTTGATGCTGATGGTGA+CGG - Chr1:29834577-29834596 MsG0180001954.01.T01:CDS 45.0%
! GCGAAGCTCCTCATCTTTTT+CGG + Chr1:29834439-29834458 None:intergenic 45.0%
! TAAATGGCTCTACTGACTGC+AGG + Chr1:29836184-29836203 None:intergenic 45.0%
! TGCTTCGTTCTGCTTTGTTC+AGG + Chr1:29837363-29837382 None:intergenic 45.0%
! TGGGAAGGATGACCCTTTTT+GGG - Chr1:29837857-29837876 MsG0180001954.01.T01:intron 45.0%
!! CTGCCGATACCGATTTGTAA+CGG - Chr1:29837751-29837770 MsG0180001954.01.T01:intron 45.0%
!!! ATCTTTTTCGGTTGCAGCAC+CGG + Chr1:29834427-29834446 None:intergenic 45.0%
!!! GGCCTTCTCGTCCTTTTTTT+AGG - Chr1:29836608-29836627 MsG0180001954.01.T01:intron 45.0%
!! AATTTCAAAATAAAATAAAA+AGG + Chr1:29834679-29834698 None:intergenic 5.0%
!! TATTTATTATGTTATAATTA+TGG + Chr1:29836997-29837016 None:intergenic 5.0%
!! TTAAATCATATAATATATTA+GGG + Chr1:29835543-29835562 None:intergenic 5.0%
!! TTTAAATCATATAATATATT+AGG + Chr1:29835544-29835563 None:intergenic 5.0%
!!! TTTTTCTTTTTTTATAATTT+TGG + Chr1:29836110-29836129 None:intergenic 5.0%
AAGTCTGTAACGGCGTTTCG+CGG - Chr1:29837622-29837641 MsG0180001954.01.T01:intron 50.0%
ACGACAGGCACACGATTCAT+GGG + Chr1:29835229-29835248 None:intergenic 50.0%
AGCAGGGTTCCACAATACAG+TGG + Chr1:29836698-29836717 None:intergenic 50.0%
ATGGACTTACCCTCTGACTC+AGG - Chr1:29838113-29838132 MsG0180001954.01.T01:intron 50.0%
ATTGTGGAACCCTGCTACCA+GGG - Chr1:29836702-29836721 MsG0180001954.01.T01:intron 50.0%
ATTTGTAACGGCCGCAATCC+CGG - Chr1:29837763-29837782 MsG0180001954.01.T01:intron 50.0%
CAAAAAGGGTCATCCTTCCC+AGG + Chr1:29837858-29837877 None:intergenic 50.0%
CAGGTGAAAGGTTTGAGGAC+AGG - Chr1:29836572-29836591 MsG0180001954.01.T01:intron 50.0%
CATTCTCTCACAGTCACCGA+GGG + Chr1:29834553-29834572 None:intergenic 50.0%
CGGCCGTTACAAATCGGTAT+CGG + Chr1:29837757-29837776 None:intergenic 50.0%
CTTTACGACGTCGACAAGGA+CGG - Chr1:29834471-29834490 MsG0180001954.01.T01:CDS 50.0%
GACTTAAAGTCCCTGGTAGC+AGG + Chr1:29836715-29836734 None:intergenic 50.0%
GCACACGATTCATGGGATAG+TGG + Chr1:29835222-29835241 None:intergenic 50.0%
GCAGGTTGTGCTTGCTTCTT+GGG + Chr1:29836166-29836185 None:intergenic 50.0%
GCCTCAGAAGTAACTCTTGG+AGG - Chr1:29837958-29837977 MsG0180001954.01.T01:intron 50.0%
GCGAGAAACAATAGCACGAC+AGG + Chr1:29835244-29835263 None:intergenic 50.0%
GGATTGCGGCCGTTACAAAT+CGG + Chr1:29837763-29837782 None:intergenic 50.0%
GTCCATGATTCCTTGACACC+GGG + Chr1:29838248-29838267 None:intergenic 50.0%
GTCCTACCTGGCCAAGAAAT+AGG + Chr1:29838005-29838024 None:intergenic 50.0%
TATTGTGGAACCCTGCTACC+AGG - Chr1:29836701-29836720 MsG0180001954.01.T01:intron 50.0%
TCATTCTCTCACAGTCACCG+AGG + Chr1:29834554-29834573 None:intergenic 50.0%
TGACGGGAAAGTCAACTTCG+AGG - Chr1:29834594-29834613 MsG0180001954.01.T01:CDS 50.0%
TGCAGGTTGTGCTTGCTTCT+TGG + Chr1:29836167-29836186 None:intergenic 50.0%
TGGGAAAAGAGTTCACGCAG+GGG - Chr1:29835596-29835615 MsG0180001954.01.T01:intron 50.0%
TGTCGCGATTTCGGCTGTAA+CGG - Chr1:29836853-29836872 MsG0180001954.01.T01:intron 50.0%
TTGGGTTTATGTGCCTGGGA+AGG - Chr1:29837842-29837861 MsG0180001954.01.T01:intron 50.0%
TTTGCCGATGTCTACTGCAC+CGG - Chr1:29834405-29834424 MsG0180001954.01.T01:CDS 50.0%
! AACCCGGTGTCAAGGAATCA+TGG - Chr1:29838243-29838262 MsG0180001954.01.T01:CDS 50.0%
! AGAAGATGATGGCTCGCTGA+TGG - Chr1:29834623-29834642 MsG0180001954.01.T01:CDS 50.0%
! CTGGGAAGGATGACCCTTTT+TGG - Chr1:29837856-29837875 MsG0180001954.01.T01:intron 50.0%
! TCATGATGATGACACACGCC+TGG - Chr1:29836523-29836542 MsG0180001954.01.T01:intron 50.0%
! TGCCTATTTCTTGGCCAGGT+AGG - Chr1:29838000-29838019 MsG0180001954.01.T01:intron 50.0%
!! TCTCTTCTTTGGTGCTGGAC+TGG + Chr1:29837495-29837514 None:intergenic 50.0%
!! TGATTGGTGCCAGAGTTGCT+TGG - Chr1:29838145-29838164 MsG0180001954.01.T01:intron 50.0%
AAATCGCCGTACGGCCGTTA+CGG + Chr1:29837680-29837699 None:intergenic 55.0%
AGGACCAAAAACGGTCACGG+TGG - Chr1:29837728-29837747 MsG0180001954.01.T01:intron 55.0%
CACGACAGGCACACGATTCA+TGG + Chr1:29835230-29835249 None:intergenic 55.0%
CGACCTTTACGACGTCGACA+AGG - Chr1:29834467-29834486 MsG0180001954.01.T01:CDS 55.0%
CGTCCTTGTCGACGTCGTAA+AGG + Chr1:29834473-29834492 None:intergenic 55.0%
TGACACACGCCTGGTCGTAA+AGG - Chr1:29836532-29836551 MsG0180001954.01.T01:intron 55.0%
! TTTGGGTCAACCCGGTGTCA+AGG - Chr1:29838235-29838254 MsG0180001954.01.T01:intron 55.0%
!! AGCACCGGTGCAGTAGACAT+CGG + Chr1:29834412-29834431 None:intergenic 55.0%
CCCGTAACGGCGTTCCGTAA+CGG - Chr1:29837663-29837682 MsG0180001954.01.T01:intron 60.0%
CCGTTACGGAACGCCGTTAC+GGG + Chr1:29837666-29837685 None:intergenic 60.0%
GCCGTTACGGAACGCCGTTA+CGG + Chr1:29837667-29837686 None:intergenic 60.0%
! GCAGCCACCGTGACCGTTTT+TGG + Chr1:29837735-29837754 None:intergenic 60.0%
AACGGCCGCAATCCCGGTAC+CGG - Chr1:29837769-29837788 MsG0180001954.01.T01:intron 65.0%
ACGGCGTTTCGCGCCCGTAA+CGG - Chr1:29837650-29837669 MsG0180001954.01.T01:intron 65.0%
ACGGCGTTTCGCGGCCGTAA+CGG - Chr1:29837631-29837650 MsG0180001954.01.T01:intron 65.0%
ACGGGCGCGAAACGCCGTTA+CGG + Chr1:29837648-29837667 None:intergenic 65.0%
GGCGTTCCGTAACGGCCGTA+CGG - Chr1:29837671-29837690 MsG0180001954.01.T01:intron 65.0%
TCGCGATCGCGGTCGCGTTA+AGG - Chr1:29836829-29836848 MsG0180001954.01.T01:intron 65.0%
! ACGGACCGGTACCGGGATTG+CGG + Chr1:29837777-29837796 None:intergenic 65.0%
AACGGTCGCGGTCGCGATCG+CGG - Chr1:29836818-29836837 MsG0180001954.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 29834306 29838490 29834306 ID=MsG0180001954.01;Name=MsG0180001954.01
Chr1 mRNA 29834306 29838490 29834306 ID=MsG0180001954.01.T01;Parent=MsG0180001954.01;Name=MsG0180001954.01.T01;_AED=0.18;_eAED=0.18;_QI=0|0|0|1|1|1|3|0|362
Chr1 exon 29838241 29838490 29838241 ID=MsG0180001954.01.T01:exon:42405;Parent=MsG0180001954.01.T01
Chr1 exon 29836010 29836221 29836010 ID=MsG0180001954.01.T01:exon:42404;Parent=MsG0180001954.01.T01
Chr1 exon 29834306 29834932 29834306 ID=MsG0180001954.01.T01:exon:42403;Parent=MsG0180001954.01.T01
Chr1 CDS 29838241 29838490 29838241 ID=MsG0180001954.01.T01:cds;Parent=MsG0180001954.01.T01
Chr1 CDS 29836010 29836221 29836010 ID=MsG0180001954.01.T01:cds;Parent=MsG0180001954.01.T01
Chr1 CDS 29834306 29834932 29834306 ID=MsG0180001954.01.T01:cds;Parent=MsG0180001954.01.T01
Gene Sequence

>MsG0180001954.01.T01

ATGCTTTCAACACTTGGAAGCAAACCAACATTTGATGAAGTAATAAAGATGATGGAAGATATTGATCAGAACGGCGACGGTTATGTTGATCTTAAAGAATTTGCCGATGTCTACTGCACCGGTGCTGCAACCGAAAAAGATGAGGAGCTTCGCGATGCTTTCGACCTTTACGACGTCGACAAGGACGGTCTCATCTCTCCGAAAGAATTGCACATAGTTCTTAATAAAATTGGTGAGAAATACTCCCTCGGTGAAAGGTTTGAGGACAGGGTTAGATTAGACTGGCCTTCTCGTCCTTTTTTTAGGATTTTCGGTTCGGTTAGTGTTAATGGCACTCTTTGTCTCTGCAAAGACTATGGTTTTTACACAACCACTGTATTGTGGAACCCTGCTACCAGGGACTTTAAGTCCATTCCTCCTACCCAACCATTTGAGAACATTGAGTTGAACATTGACTATAAGGATGACCCTTTTTGGGAGACTAATGTGAATGACCATTATTGGGAGTTGAAGGAGGTCACAGTCCAGAAGTATGACCCTTTCTGTGAGATATATAGCCTCAGAAGTAACTCTTGGAGGAAACTTGAAGGGGTTGATATGCCTATTTCTTGGCCAGGTAGGACTCATGTGAACTTGAATGGATTTTTCTATTGGTTTAGCCCAGGAAATGTTATGATGTCATTTGACTTTAGCAAAGAGATATTTTTTGCAATGGACTTACCCTCTGACTCAGGTGTAATACTTGATTGGTGCCAGAGTTGCTTGGTGATGTTAAATGGGTCTGTTTCTCTCATCTCTAATCATGACAAGACACCTTATTTTCACATATGGATTTTGGGTCAACCCGGTGTCAAGGAATCATGGACTAAAGTTTTCGTTGTTGGACCATTAAGTTATGCTATGAGTCCTATCGGAACAGTGAATAAGAGCCATAGATTCTTTAGGAAAGACGAAGAAGGTTGTGAGCTAGTTAGCTTTGATATTAGAACTCAAAGGATTGCAGAAATTGAGGTTAAGGGAAACCTGCTTGCAACGGATGTTGTAATTTACAAGGAAAATCTTCTTCCTTTTGGAGGAATGAGCCGTTAA
Protein sequence

>MsG0180001954.01.T01

MLSTLGSKPTFDEVIKMMEDIDQNGDGYVDLKEFADVYCTGAATEKDEELRDAFDLYDVDKDGLISPKELHIVLNKIGEKYSLGERFEDRVRLDWPSRPFFRIFGSVSVNGTLCLCKDYGFYTTTVLWNPATRDFKSIPPTQPFENIELNIDYKDDPFWETNVNDHYWELKEVTVQKYDPFCEIYSLRSNSWRKLEGVDMPISWPGRTHVNLNGFFYWFSPGNVMMSFDFSKEIFFAMDLPSDSGVILDWCQSCLVMLNGSVSLISNHDKTPYFHIWILGQPGVKESWTKVFVVGPLSYAMSPIGTVNKSHRFFRKDEEGCELVSFDIRTQRIAEIEVKGNLLATDVVIYKENLLPFGGMSR*