AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045001.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045001.01.T01 MTR_8g063350 73.724 392 53 5 1 390 1 344 0.0 556
MsG0880045001.01.T01 MTR_8g063370 59.591 391 143 9 1 384 11 393 1.44e-145 419
MsG0880045001.01.T01 MTR_8g063310 51.980 404 160 11 1 384 9 398 5.86e-124 364
MsG0880045001.01.T01 MTR_2g034700 41.176 391 199 12 1 379 1 372 5.46e-81 253
MsG0880045001.01.T01 MTR_3g011120 41.600 375 186 12 13 372 13 369 3.74e-78 246
MsG0880045001.01.T01 MTR_8g064890 41.495 388 188 11 8 374 10 379 8.40e-78 245
MsG0880045001.01.T01 MTR_2g021800 36.124 418 209 15 2 375 5 408 2.20e-72 232
MsG0880045001.01.T01 MTR_8g063440 39.370 381 191 14 14 373 12 373 2.65e-72 231
MsG0880045001.01.T01 MTR_8g467670 37.500 392 205 12 6 379 6 375 3.09e-72 231
MsG0880045001.01.T01 MTR_2g066200 39.031 392 186 18 14 375 12 380 9.90e-69 222
MsG0880045001.01.T01 MTR_8g036970 39.554 359 148 9 18 363 2 304 2.23e-68 220
MsG0880045001.01.T01 MTR_2g066170 37.659 393 204 13 5 374 3 377 8.40e-68 219
MsG0880045001.01.T01 MTR_2g022000 35.012 417 218 16 2 377 5 409 1.27e-67 220
MsG0880045001.01.T01 MTR_0129s0070 38.462 390 194 14 14 375 17 388 2.07e-67 219
MsG0880045001.01.T01 MTR_8g039500 38.356 365 186 14 5 351 3 346 4.27e-66 214
MsG0880045001.01.T01 MTR_8g064790 40.157 381 163 12 4 374 3 328 5.45e-66 213
MsG0880045001.01.T01 MTR_0129s0050 36.855 407 192 18 14 375 14 400 7.06e-65 213
MsG0880045001.01.T01 MTR_0129s0120 36.935 398 205 14 6 375 9 388 3.59e-64 210
MsG0880045001.01.T01 MTR_4g028510 37.975 395 206 14 8 374 10 393 3.64e-63 209
MsG0880045001.01.T01 MTR_8g464770 35.809 377 211 12 14 375 12 372 7.54e-63 206
MsG0880045001.01.T01 MTR_2g066150 36.412 379 202 14 5 374 3 351 1.86e-62 205
MsG0880045001.01.T01 MTR_2g065840 37.143 385 206 15 13 375 11 381 4.92e-62 204
MsG0880045001.01.T01 MTR_6g080680 37.596 391 197 15 11 374 16 386 5.77e-62 204
MsG0880045001.01.T01 MTR_5g024260 37.056 394 199 13 8 372 16 389 2.26e-61 203
MsG0880045001.01.T01 MTR_4g028750 37.795 381 198 15 8 362 10 377 8.17e-61 201
MsG0880045001.01.T01 MTR_4g014370 34.826 402 211 14 14 375 11 401 9.16e-61 202
MsG0880045001.01.T01 MTR_7g069550 35.577 416 200 19 1 374 1 390 8.41e-60 199
MsG0880045001.01.T01 MTR_4g028660 36.132 393 215 15 8 375 10 391 2.00e-59 198
MsG0880045001.01.T01 MTR_4g027450 36.132 393 215 15 8 375 10 391 2.00e-59 198
MsG0880045001.01.T01 MTR_2g065860 36.434 387 203 15 13 374 11 379 2.69e-59 197
MsG0880045001.01.T01 MTR_1g098180 35.825 388 185 13 9 375 14 358 7.19e-59 196
MsG0880045001.01.T01 MTR_4g028680 37.340 391 214 14 8 375 10 392 9.85e-59 196
MsG0880045001.01.T01 MTR_5g097330 34.080 402 217 15 14 383 16 401 2.94e-58 195
MsG0880045001.01.T01 MTR_4g130330 37.722 395 203 19 10 375 3 383 8.12e-58 194
MsG0880045001.01.T01 MTR_0054s0100 34.474 409 223 15 1 374 1 399 8.74e-57 191
MsG0880045001.01.T01 MTR_5g043605 35.620 379 163 14 11 376 22 332 1.16e-56 190
MsG0880045001.01.T01 MTR_6g086825 37.065 402 196 17 13 375 69 452 1.29e-56 192
MsG0880045001.01.T01 MTR_0054s0090 35.533 394 210 15 13 374 16 397 1.51e-56 191
MsG0880045001.01.T01 MTR_0134s0060 33.820 411 217 15 6 373 4 402 1.52e-56 191
MsG0880045001.01.T01 MTR_5g097220 35.381 407 213 17 14 383 13 406 1.53e-56 191
MsG0880045001.01.T01 MTR_6g079810 34.091 396 217 13 12 373 68 453 1.41e-55 189
MsG0880045001.01.T01 MTR_6g090180 34.988 403 218 15 6 375 39 430 1.73e-55 189
MsG0880045001.01.T01 MTR_0831s0010 34.309 376 192 13 9 355 45 394 2.65e-55 188
MsG0880045001.01.T01 MTR_7g079640 35.878 393 195 18 7 374 6 366 2.89e-55 187
MsG0880045001.01.T01 MTR_2g021950 35.385 390 208 18 14 375 16 389 1.65e-54 185
MsG0880045001.01.T01 MTR_8g063330 34.551 356 188 8 9 335 9 348 3.06e-54 186
MsG0880045001.01.T01 MTR_5g092850 32.754 403 228 13 10 383 12 400 3.47e-54 184
MsG0880045001.01.T01 MTR_8g032980 33.573 417 203 16 14 375 16 413 8.85e-54 184
MsG0880045001.01.T01 MTR_2g064950 33.668 398 201 18 14 374 10 381 1.10e-53 183
MsG0880045001.01.T01 MTR_5g096910 33.419 389 203 16 1 356 1 366 2.04e-53 182
MsG0880045001.01.T01 MTR_2g038680 34.286 385 195 16 14 356 16 384 2.81e-53 182
MsG0880045001.01.T01 MTR_7g017130 32.653 392 214 18 14 375 18 389 1.01e-52 180
MsG0880045001.01.T01 MTR_2g021770 32.506 403 210 16 17 375 15 399 1.03e-52 181
MsG0880045001.01.T01 MTR_7g017170 33.244 373 200 17 14 356 18 371 1.56e-52 180
MsG0880045001.01.T01 MTR_4g102410 34.465 383 208 15 6 356 53 424 1.81e-52 181
MsG0880045001.01.T01 MTR_5g097680 32.506 403 215 13 11 363 14 409 2.45e-52 180
MsG0880045001.01.T01 MTR_4g055820 33.154 371 199 13 1 335 1 358 2.83e-52 178
MsG0880045001.01.T01 MTR_6g461660 35.450 378 201 16 6 355 11 373 2.89e-52 179
MsG0880045001.01.T01 MTR_1g007410 34.761 397 208 17 14 377 22 400 5.02e-52 179
MsG0880045001.01.T01 MTR_7g017040 35.806 391 197 18 1 356 1 372 5.89e-52 178
MsG0880045001.01.T01 MTR_6g079770 34.398 407 208 15 13 374 16 408 8.17e-52 178
MsG0880045001.01.T01 MTR_5g037680 33.417 398 202 15 14 375 8 378 8.91e-52 179
MsG0880045001.01.T01 MTR_1g053510 32.143 420 218 16 1 375 1 398 9.43e-52 178
MsG0880045001.01.T01 MTR_8g012090 32.865 356 184 11 14 351 16 334 1.71e-51 175
MsG0880045001.01.T01 MTR_1g050308 33.506 385 172 12 5 375 25 339 3.58e-51 175
MsG0880045001.01.T01 MTR_5g091140 32.048 415 212 16 6 385 40 419 9.53e-51 176
MsG0880045001.01.T01 MTR_8g013000 35.196 358 181 15 14 336 10 351 8.11e-50 173
MsG0880045001.01.T01 MTR_5g096630 32.982 379 204 16 17 356 21 388 9.43e-50 173
MsG0880045001.01.T01 MTR_5g097350 32.552 384 215 17 23 384 25 386 1.20e-49 172
MsG0880045001.01.T01 MTR_8g046310 33.162 389 217 16 14 385 16 378 1.65e-49 171
MsG0880045001.01.T01 MTR_5g059430 30.900 411 227 14 8 374 20 417 1.82e-49 172
MsG0880045001.01.T01 MTR_1g007450 33.693 371 204 14 14 355 12 369 2.28e-48 169
MsG0880045001.01.T01 MTR_8g046200 32.333 433 200 18 8 375 10 414 9.12e-48 168
MsG0880045001.01.T01 MTR_6g478230 34.988 403 203 16 8 375 21 399 2.21e-47 167
MsG0880045001.01.T01 MTR_5g072060 33.511 376 182 15 14 356 6 346 7.15e-47 169
MsG0880045001.01.T01 MTR_4g117550 31.458 391 213 14 6 375 9 365 2.35e-46 163
MsG0880045001.01.T01 MTR_2g104340 29.457 387 226 12 1 356 1 371 1.09e-45 162
MsG0880045001.01.T01 MTR_4g007940 35.714 308 149 11 78 355 27 315 2.44e-45 159
MsG0880045001.01.T01 MTR_5g069390 31.948 385 211 14 8 355 20 390 5.94e-45 162
MsG0880045001.01.T01 MTR_5g074850 33.095 420 228 22 8 390 29 432 6.94e-45 166
MsG0880045001.01.T01 MTR_2g104280 31.265 419 206 17 14 381 14 401 8.31e-45 159
MsG0880045001.01.T01 MTR_6g078010 33.333 354 197 13 50 385 4 336 2.82e-44 157
MsG0880045001.01.T01 MTR_8g033140 30.913 427 203 17 9 374 14 409 5.69e-44 157
MsG0880045001.01.T01 MTR_6g090250 33.081 396 205 14 32 375 29 416 8.76e-44 157
MsG0880045001.01.T01 MTR_8g033110 29.724 434 231 17 1 382 1 412 7.60e-43 154
MsG0880045001.01.T01 MTR_1g050312 36.426 291 137 12 78 356 15 269 3.78e-42 149
MsG0880045001.01.T01 MTR_8g033090 30.861 418 222 18 12 382 12 409 1.92e-41 151
MsG0880045001.01.T01 MTR_5g075370 32.468 385 206 19 9 354 3 372 2.52e-41 155
MsG0880045001.01.T01 MTR_8g046220 30.412 388 201 13 14 356 17 380 3.11e-41 150
MsG0880045001.01.T01 MTR_8g041270 31.392 395 191 19 14 356 15 381 8.46e-41 149
MsG0880045001.01.T01 MTR_1g007430 32.440 336 177 12 25 333 7 319 3.33e-40 145
MsG0880045001.01.T01 MTR_8g018110 30.508 413 215 19 12 382 12 394 4.07e-40 147
MsG0880045001.01.T01 MTR_7g016930 35.294 323 162 15 8 311 10 304 7.59e-40 145
MsG0880045001.01.T01 MTR_5g068490 34.014 294 158 11 88 375 6 269 1.12e-39 142
MsG0880045001.01.T01 MTR_5g074800 32.394 355 187 20 54 375 2 336 6.98e-38 139
MsG0880045001.01.T01 MTR_5g092860 31.667 360 190 15 32 362 20 352 1.21e-36 137
MsG0880045001.01.T01 MTR_6g087620 33.013 312 156 13 79 371 1 278 3.10e-36 134
MsG0880045001.01.T01 MTR_5g022850 29.923 391 198 16 12 381 14 349 4.50e-36 135
MsG0880045001.01.T01 MTR_5g064700 32.622 328 166 15 79 363 35 350 6.22e-36 135
MsG0880045001.01.T01 MTR_7g111700 30.073 409 193 17 14 386 15 366 2.22e-35 133
MsG0880045001.01.T01 MTR_5g096900 33.537 328 172 14 5 303 3 313 1.05e-34 130
MsG0880045001.01.T01 MTR_6g079790 35.165 273 141 11 129 375 39 301 3.66e-34 129
MsG0880045001.01.T01 MTR_7g104710 33.016 315 151 12 6 296 9 287 1.38e-32 125
MsG0880045001.01.T01 MTR_5g094350 33.333 267 140 10 11 255 10 260 1.80e-32 123
MsG0880045001.01.T01 MTR_3g018750 34.979 243 133 8 162 383 1 239 2.52e-32 122
MsG0880045001.01.T01 MTR_7g023800 32.588 313 154 12 77 374 32 302 7.01e-32 122
MsG0880045001.01.T01 MTR_5g076490 32.817 323 166 12 79 375 31 328 4.91e-31 121
MsG0880045001.01.T01 MTR_5g097820 31.227 269 143 12 10 254 19 269 1.10e-30 118
MsG0880045001.01.T01 MTR_5g086590 31.513 238 138 8 125 354 11 231 4.17e-30 116
MsG0880045001.01.T01 MTR_2g021920 37.500 240 111 12 164 375 37 265 1.90e-29 115
MsG0880045001.01.T01 MTR_6g004450 35.897 234 125 10 154 375 41 261 2.70e-29 115
MsG0880045001.01.T01 MTR_6g009350 38.710 186 94 8 165 336 9 188 1.41e-28 110
MsG0880045001.01.T01 MTR_4g014370 36.979 192 114 3 188 375 141 329 1.51e-28 114
MsG0880045001.01.T01 MTR_4g014370 37.288 118 60 4 14 119 22 137 5.80e-16 78.6
MsG0880045001.01.T01 MTR_4g016000 37.158 183 90 5 161 333 26 193 1.97e-28 111
MsG0880045001.01.T01 MTR_8g033000 28.267 375 199 18 51 375 1 355 2.34e-28 114
MsG0880045001.01.T01 MTR_6g090260 41.317 167 84 5 197 355 75 235 8.20e-28 110
MsG0880045001.01.T01 MTR_5g023970 37.195 164 92 3 196 354 10 167 2.43e-27 107
MsG0880045001.01.T01 MTR_1g076810 34.043 235 129 10 14 233 18 241 4.14e-27 113
MsG0880045001.01.T01 MTR_8g073570 37.288 177 96 6 6 175 2 170 1.03e-26 105
MsG0880045001.01.T01 MTR_7g019250 37.089 213 90 7 162 355 222 409 1.81e-26 110
MsG0880045001.01.T01 MTR_8g035690 33.696 276 126 14 139 375 38 295 1.40e-25 105
MsG0880045001.01.T01 MTR_5g097850 36.765 204 91 8 168 356 40 220 3.41e-25 103
MsG0880045001.01.T01 MTR_1g115205 32.283 254 96 13 14 238 29 235 2.40e-24 100
MsG0880045001.01.T01 MTR_5g098950 33.333 219 99 10 129 336 90 272 8.67e-24 100
MsG0880045001.01.T01 MTR_6g070880 34.335 233 122 11 7 212 26 254 3.73e-23 97.8
MsG0880045001.01.T01 MTR_6g071420 34.335 233 122 11 7 212 26 254 3.73e-23 97.8
MsG0880045001.01.T01 MTR_6g465360 32.773 238 125 11 6 211 54 288 3.76e-23 98.6
MsG0880045001.01.T01 MTR_7g101200 30.472 233 123 11 122 344 33 236 4.73e-23 97.1
MsG0880045001.01.T01 MTR_5g097650 39.416 137 78 3 13 148 21 153 2.79e-22 92.8
MsG0880045001.01.T01 MTR_5g037190 28.148 270 132 12 14 256 16 250 1.23e-21 93.6
MsG0880045001.01.T01 MTR_3g019290 31.754 211 103 7 1 203 1 178 4.22e-21 90.1
MsG0880045001.01.T01 MTR_5g097540 36.000 150 83 4 196 341 96 236 8.45e-21 92.0
MsG0880045001.01.T01 MTR_1g037540 25.197 381 215 15 17 348 8 367 1.79e-19 89.7
MsG0880045001.01.T01 MTR_7g017150 32.178 202 118 8 189 379 70 263 1.26e-18 85.5
MsG0880045001.01.T01 MTR_5g096920 37.692 130 72 4 232 356 1 126 1.38e-18 82.4
MsG0880045001.01.T01 MTR_6g079730 40.909 132 69 3 250 375 14 142 2.65e-18 81.6
MsG0880045001.01.T01 MTR_2g104260 28.674 279 144 12 14 257 14 272 5.51e-18 83.6
MsG0880045001.01.T01 MTR_2g046830 27.034 381 203 21 5 347 13 356 5.95e-18 85.1
MsG0880045001.01.T01 MTR_3g030620 32.317 164 95 5 229 380 20 179 9.00e-18 80.9
MsG0880045001.01.T01 MTR_2g046920 24.800 375 219 20 5 347 12 355 9.02e-18 84.3
MsG0880045001.01.T01 MTR_7g017120 32.338 201 118 8 189 379 70 262 1.11e-17 82.8
MsG0880045001.01.T01 MTR_3g047580 38.298 141 57 4 1 133 1 119 1.39e-17 79.0
MsG0880045001.01.T01 MTR_2g022020 42.342 111 49 4 275 381 17 116 1.48e-17 78.6
MsG0880045001.01.T01 MTR_8g101580 24.339 378 209 17 22 363 54 390 1.92e-17 83.6
MsG0880045001.01.T01 MTR_2g021155 24.786 351 222 16 14 354 36 354 2.34e-17 84.7
MsG0880045001.01.T01 MTR_5g066420 37.037 135 72 3 232 355 1 133 2.26e-16 76.3
MsG0880045001.01.T01 MTR_2g029990 25.397 315 189 15 17 315 54 338 6.78e-16 79.0
MsG0880045001.01.T01 MTR_1g094210 24.796 367 219 17 5 353 48 375 1.65e-15 77.8
MsG0880045001.01.T01 MTR_2g047832 24.810 395 214 21 5 347 17 380 1.88e-15 77.8
MsG0880045001.01.T01 MTR_1g097560 23.514 387 244 17 1 368 23 376 3.70e-15 76.6
MsG0880045001.01.T01 MTR_1g094210 25.625 320 190 15 5 313 48 330 6.10e-15 76.3
MsG0880045001.01.T01 MTR_2g021150 24.465 327 204 11 13 323 13 312 6.58e-15 75.9
MsG0880045001.01.T01 MTR_1g097330 24.855 346 205 15 14 339 16 326 7.38e-15 75.9
MsG0880045001.01.T01 MTR_5g097660 48.889 90 41 3 268 355 7 93 1.27e-14 70.5
MsG0880045001.01.T01 MTR_2g046900 23.861 373 216 18 13 348 27 368 2.10e-14 74.3
MsG0880045001.01.T01 MTR_4g133170 22.671 322 211 15 1 316 1 290 2.83e-14 74.3
MsG0880045001.01.T01 MTR_5g096070 31.953 169 80 8 8 171 20 158 7.22e-14 69.3
MsG0880045001.01.T01 MTR_1g068860 24.620 329 199 18 17 327 7 304 7.64e-14 72.8
MsG0880045001.01.T01 MTR_2g021190 26.214 309 194 16 17 315 24 308 9.77e-14 72.4
MsG0880045001.01.T01 MTR_7g407070 25.538 325 196 14 17 316 35 338 2.07e-13 71.6
MsG0880045001.01.T01 MTR_2g024110 22.018 327 220 10 14 329 4 306 2.49e-13 71.2
MsG0880045001.01.T01 MTR_5g091150 29.612 206 98 10 102 301 35 199 2.73e-13 68.6
MsG0880045001.01.T01 MTR_2g031720 25.266 376 211 22 13 355 5 343 2.89e-13 71.2
MsG0880045001.01.T01 MTR_3g009310 25.478 314 199 15 17 321 45 332 3.27e-13 70.9
MsG0880045001.01.T01 MTR_4g055060 24.260 338 203 16 17 336 33 335 4.18e-13 70.5
MsG0880045001.01.T01 MTR_3g013770 27.190 331 181 20 17 324 36 329 4.40e-13 70.5
MsG0880045001.01.T01 MTR_4g061340 25.000 324 201 15 17 325 29 325 8.24e-13 69.7
MsG0880045001.01.T01 MTR_3g058480 26.360 239 141 10 17 241 42 259 1.07e-12 68.9
MsG0880045001.01.T01 MTR_1g097490 24.586 362 230 17 5 355 40 369 1.10e-12 69.3
MsG0880045001.01.T01 MTR_5g036250 35.965 114 70 2 269 382 1 111 1.47e-12 66.2
MsG0880045001.01.T01 MTR_3g036335 22.222 378 238 15 17 383 44 376 1.70e-12 68.6
MsG0880045001.01.T01 MTR_2g021252 22.767 347 202 16 17 346 50 347 2.28e-12 68.2
MsG0880045001.01.T01 MTR_3g007360 25.159 314 182 15 22 316 60 339 2.30e-12 68.6
MsG0880045001.01.T01 MTR_3g006670 24.290 317 196 14 17 318 52 339 2.31e-12 68.6
MsG0880045001.01.T01 MTR_1g009110 24.706 340 202 16 6 319 6 317 2.58e-12 68.2
MsG0880045001.01.T01 MTR_1g050305 49.383 81 34 3 281 356 21 99 2.68e-12 64.3
MsG0880045001.01.T01 MTR_2g028140 22.727 352 204 16 17 327 8 332 2.70e-12 68.2
MsG0880045001.01.T01 MTR_3g036290 24.458 323 204 12 17 334 25 312 2.91e-12 67.8
MsG0880045001.01.T01 MTR_2g055970 26.230 366 199 19 13 344 210 538 2.96e-12 68.6
MsG0880045001.01.T01 MTR_2g025420 23.739 337 198 14 17 316 9 323 3.87e-12 67.8
MsG0880045001.01.T01 MTR_4g037465 24.779 339 196 15 7 316 14 322 5.31e-12 67.4
MsG0880045001.01.T01 MTR_3g009240 23.647 351 228 13 17 360 39 356 7.83e-12 66.6
MsG0880045001.01.T01 MTR_3g058470 25.287 348 179 14 17 344 40 326 8.65e-12 66.6
MsG0880045001.01.T01 MTR_1g068875 21.865 311 206 13 17 316 7 291 8.79e-12 66.6
MsG0880045001.01.T01 MTR_3g007330 24.786 351 206 17 22 344 56 376 1.25e-11 66.2
MsG0880045001.01.T01 MTR_7g070440 25.826 333 188 18 12 316 5 306 1.93e-11 65.5
MsG0880045001.01.T01 MTR_4g070550 24.324 333 195 18 17 316 8 316 2.77e-11 65.1
MsG0880045001.01.T01 MTR_0064s0160 23.513 353 222 22 14 334 42 378 3.11e-11 65.1
MsG0880045001.01.T01 MTR_0667s0010 20.635 315 217 9 17 315 9 306 3.94e-11 64.7
MsG0880045001.01.T01 MTR_3g034130 22.581 372 246 15 10 362 5 353 5.63e-11 63.9
MsG0880045001.01.T01 MTR_8g467140 24.194 310 199 13 13 312 31 314 6.15e-11 63.5
MsG0880045001.01.T01 MTR_5g070080 22.161 361 232 12 7 339 3 342 6.27e-11 63.9
MsG0880045001.01.T01 MTR_5g086780 65.854 41 14 0 14 54 16 56 8.43e-11 62.0
MsG0880045001.01.T01 MTR_5g070088 23.457 324 206 10 12 311 5 310 8.66e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045001.01.T01 AT4G22390 22.812 320 211 12 89 384 71 378 2.29e-11 65.5
MsG0880045001.01.T01 AT4G22390 22.812 320 211 12 89 384 71 378 2.39e-11 65.5

Find 65 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTATGGCTATAATGATTT+AGG 0.169187 8:+52597245 MsG0880045001.01.T01:CDS
TTGAAATTGGTGGTGTTAAA+TGG 0.199974 8:+52597518 MsG0880045001.01.T01:CDS
ATTCAACTTGTGAATTATTA+TGG 0.217033 8:+52597230 MsG0880045001.01.T01:CDS
GATAGATTTAAGAGGAAGTT+TGG 0.264442 8:-52596769 None:intergenic
TTTCTTCTGCTTGCAAATCT+TGG 0.315310 8:+52596799 MsG0880045001.01.T01:CDS
ATCATCATCATCATGAGATT+TGG 0.319862 8:-52596877 None:intergenic
AACTGAATCGTCTTTGAAAT+TGG 0.323062 8:+52597505 MsG0880045001.01.T01:CDS
ATGTAAACCTCCAAACCATT+AGG 0.331465 8:-52597371 None:intergenic
TCATAAGATTCCATTTCTTC+AGG 0.346334 8:+52597343 MsG0880045001.01.T01:CDS
GATAGGTTGTATTTGCGTTC+TGG 0.359347 8:+52596927 MsG0880045001.01.T01:CDS
TCAGGATAACCCTAATGGTT+TGG 0.364228 8:+52597361 MsG0880045001.01.T01:CDS
TTTCTTCAGGATAACCCTAA+TGG 0.382039 8:+52597356 MsG0880045001.01.T01:CDS
CCACCAACTGGCAAGAATAA+TGG 0.384394 8:+52596708 MsG0880045001.01.T01:CDS
TTTAAGGATCCTATCTCAGC+TGG 0.407616 8:+52597659 MsG0880045001.01.T01:CDS
TGTAAACCTCCAAACCATTA+GGG 0.410380 8:-52597370 None:intergenic
CCCTTCTTCCCAGCTGAGAT+AGG 0.413249 8:-52597668 None:intergenic
AATCGCTTGATAGATTTAAG+AGG 0.421126 8:-52596777 None:intergenic
TTAGGGTTATCCTGAAGAAA+TGG 0.422985 8:-52597353 None:intergenic
CCATTATTCTTGCCAGTTGG+TGG 0.426616 8:-52596708 None:intergenic
TGCAGCTGAAAGCTTAGAAT+TGG 0.431001 8:-52597465 None:intergenic
TGAAAATGCTTAGCATCAAA+TGG 0.432594 8:-52596996 None:intergenic
CACTGTTAATCATGATTTCA+AGG 0.434744 8:+52597205 MsG0880045001.01.T01:CDS
AAATCGGTGTCGGAAATTCC+TGG 0.436340 8:+52597603 MsG0880045001.01.T01:CDS
GTGATGATGATGATACCTTT+TGG 0.437422 8:+52597267 MsG0880045001.01.T01:CDS
TAATGGTTTGGAGGTTTACA+TGG 0.437521 8:+52597373 MsG0880045001.01.T01:CDS
TCCTATCTCAGCTGGGAAGA+AGG 0.438071 8:+52597667 MsG0880045001.01.T01:CDS
TGAAATTGGTGGTGTTAAAT+GGG 0.458418 8:+52597519 MsG0880045001.01.T01:CDS
TCTTCTTTCTCTTCATTGAA+AGG 0.458533 8:-52597832 None:intergenic
CTATACTGACCACTAACATC+AGG 0.467357 8:-52597095 None:intergenic
GGTTTGGAGGTTTACATGGA+TGG 0.484373 8:+52597377 MsG0880045001.01.T01:CDS
ACCTTCAGCTCAACGACGAC+CGG 0.490270 8:-52597858 None:intergenic
GGATAACCCTAATGGTTTGG+AGG 0.504009 8:+52597364 MsG0880045001.01.T01:CDS
TCAATGGCAAATCCACCAAC+TGG 0.512413 8:+52596696 None:intergenic
GAGATAGGATCCTTAAAAGA+AGG 0.529126 8:-52597653 None:intergenic
CCTATCTCAGCTGGGAAGAA+GGG 0.535589 8:+52597668 MsG0880045001.01.T01:CDS
AAGGGTGTTATATTCTTCAA+AGG 0.535738 8:+52597686 MsG0880045001.01.T01:CDS
ATTGAAATGCTAAAAGACAT+AGG 0.542826 8:-52597572 None:intergenic
ACTGACTATGCCGAGGACCT+AGG 0.545392 8:+52597128 MsG0880045001.01.T01:CDS
CTGACTATGCCGAGGACCTA+GGG 0.548230 8:+52597129 MsG0880045001.01.T01:CDS
TTGTGGAACCCTGATGTTAG+TGG 0.559281 8:+52597086 MsG0880045001.01.T01:CDS
ATCAGAAGAATATAATCTTG+TGG 0.560900 8:+52597069 MsG0880045001.01.T01:CDS
CTTTGACTCTGCCCAACCAA+TGG 0.561403 8:-52597406 None:intergenic
GAGACAATACACTTGCCAAA+AGG 0.561919 8:-52597282 None:intergenic
TGCTTAGCATCAAATGGAGA+TGG 0.578406 8:-52596990 None:intergenic
CTCTCTTTAACATCAACGAT+AGG 0.586154 8:+52596910 MsG0880045001.01.T01:CDS
TGAATCGTCTTTGAAATTGG+TGG 0.586889 8:+52597508 MsG0880045001.01.T01:CDS
AACTACCGGGATGATTGAGA+AGG 0.593822 8:+52597745 MsG0880045001.01.T01:CDS
GTACCATTATTCTTGCCAGT+TGG 0.595191 8:-52596711 None:intergenic
GTGTGGATACCCTAGGTCCT+CGG 0.595279 8:-52597138 None:intergenic
AATTTCCTTCTCAATCATCC+CGG 0.600741 8:-52597750 None:intergenic
TTCAAAGGAGATAATAACAA+TGG 0.603159 8:+52597701 MsG0880045001.01.T01:CDS
TTAAGGATCCTATCTCAGCT+GGG 0.604146 8:+52597660 MsG0880045001.01.T01:CDS
ATTGTCTCCGCACTAACAAG+TGG 0.607609 8:+52597297 MsG0880045001.01.T01:CDS
AATGGAATCTTATGACCACT+AGG 0.609719 8:-52597335 None:intergenic
AGGTCCTCGGCATAGTCAGT+AGG 0.617892 8:-52597125 None:intergenic
AGTCCTCGTGTGGATACCCT+AGG 0.620499 8:-52597145 None:intergenic
CTATCGTTGATGTTAAAGAG+AGG 0.627836 8:-52596909 None:intergenic
TATACTGACCACTAACATCA+GGG 0.638891 8:-52597094 None:intergenic
CCTCGGCATAGTCAGTAGGA+AGG 0.641727 8:-52597121 None:intergenic
ATGAAGAGAAAGAAGAACAC+CGG 0.665888 8:+52597839 MsG0880045001.01.T01:CDS
TTAGCGACGAAGTCCTCGTG+TGG 0.668780 8:-52597155 None:intergenic
ACAAAACTTAACATACCTAG+TGG 0.685183 8:+52597320 MsG0880045001.01.T01:CDS
CCTTCCTACTGACTATGCCG+AGG 0.689313 8:+52597121 MsG0880045001.01.T01:CDS
GGACCTAGGGTATCCACACG+AGG 0.717793 8:+52597142 MsG0880045001.01.T01:CDS
TTGGGCAGAGTCAAAGCACG+TGG 0.724731 8:+52597413 MsG0880045001.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTCAACTTGTGAATTATTA+TGG + Chr8:52597230-52597249 MsG0880045001.01.T01:CDS 20.0%
!! TATTATGGCTATAATGATTT+AGG + Chr8:52597245-52597264 MsG0880045001.01.T01:CDS 20.0%
!!! TCTTTTAGCATTTCAATTTT+GGG + Chr8:52597578-52597597 MsG0880045001.01.T01:CDS 20.0%
! ATCAGAAGAATATAATCTTG+TGG + Chr8:52597069-52597088 MsG0880045001.01.T01:CDS 25.0%
! ATTGAAATGCTAAAAGACAT+AGG - Chr8:52597575-52597594 None:intergenic 25.0%
! TTCAAAGGAGATAATAACAA+TGG + Chr8:52597701-52597720 MsG0880045001.01.T01:CDS 25.0%
!! AAGAACTTTTGTAAGAAACT+AGG - Chr8:52596843-52596862 None:intergenic 25.0%
!! GCTAAGCATTTTCATATTTT+AGG + Chr8:52597005-52597024 MsG0880045001.01.T01:CDS 25.0%
!! GTCTTTTAGCATTTCAATTT+TGG + Chr8:52597577-52597596 MsG0880045001.01.T01:CDS 25.0%
!!! ATTTCAATTTTGGGTCAAAT+CGG + Chr8:52597587-52597606 MsG0880045001.01.T01:CDS 25.0%
AATCGCTTGATAGATTTAAG+AGG - Chr8:52596780-52596799 None:intergenic 30.0%
ACAAAACTTAACATACCTAG+TGG + Chr8:52597320-52597339 MsG0880045001.01.T01:CDS 30.0%
ATCATCATCATCATGAGATT+TGG - Chr8:52596880-52596899 None:intergenic 30.0%
CACTGTTAATCATGATTTCA+AGG + Chr8:52597205-52597224 MsG0880045001.01.T01:CDS 30.0%
GATAGATTTAAGAGGAAGTT+TGG - Chr8:52596772-52596791 None:intergenic 30.0%
TCATAAGATTCCATTTCTTC+AGG + Chr8:52597343-52597362 MsG0880045001.01.T01:CDS 30.0%
TCTTCTTTCTCTTCATTGAA+AGG - Chr8:52597835-52597854 None:intergenic 30.0%
TGAAAATGCTTAGCATCAAA+TGG - Chr8:52596999-52597018 None:intergenic 30.0%
!! AACATTGAAAAGTTTTGTCC+AGG - Chr8:52597624-52597643 None:intergenic 30.0%
!! AACTGAATCGTCTTTGAAAT+TGG + Chr8:52597505-52597524 MsG0880045001.01.T01:CDS 30.0%
!! AAGGGTGTTATATTCTTCAA+AGG + Chr8:52597686-52597705 MsG0880045001.01.T01:CDS 30.0%
!! TGAAATTGGTGGTGTTAAAT+GGG + Chr8:52597519-52597538 MsG0880045001.01.T01:CDS 30.0%
!! TTGAAATTGGTGGTGTTAAA+TGG + Chr8:52597518-52597537 MsG0880045001.01.T01:CDS 30.0%
!! TTTTCAGGATGAAGAAGAAT+TGG + Chr8:52596956-52596975 MsG0880045001.01.T01:CDS 30.0%
!!! AATTTTCAGGTTCATGGATT+TGG + Chr8:52597176-52597195 MsG0880045001.01.T01:CDS 30.0%
!!! ATTTTAGGTTCTGCTATTCA+CGG + Chr8:52597020-52597039 MsG0880045001.01.T01:CDS 30.0%
!!! CTTGTTTTGATTTAACTACC+GGG + Chr8:52597732-52597751 MsG0880045001.01.T01:CDS 30.0%
!!! GCTTGTTTTGATTTAACTAC+CGG + Chr8:52597731-52597750 MsG0880045001.01.T01:CDS 30.0%
AATGGAATCTTATGACCACT+AGG - Chr8:52597338-52597357 None:intergenic 35.0%
AATTTCCTTCTCAATCATCC+CGG - Chr8:52597753-52597772 None:intergenic 35.0%
ATGAAGAGAAAGAAGAACAC+CGG + Chr8:52597839-52597858 MsG0880045001.01.T01:CDS 35.0%
ATGTAAACCTCCAAACCATT+AGG - Chr8:52597374-52597393 None:intergenic 35.0%
CTATCGTTGATGTTAAAGAG+AGG - Chr8:52596912-52596931 None:intergenic 35.0%
CTCTCTTTAACATCAACGAT+AGG + Chr8:52596910-52596929 MsG0880045001.01.T01:CDS 35.0%
GAGATAGGATCCTTAAAAGA+AGG - Chr8:52597656-52597675 None:intergenic 35.0%
TATACTGACCACTAACATCA+GGG - Chr8:52597097-52597116 None:intergenic 35.0%
TGTAAACCTCCAAACCATTA+GGG - Chr8:52597373-52597392 None:intergenic 35.0%
TTAGGGTTATCCTGAAGAAA+TGG - Chr8:52597356-52597375 None:intergenic 35.0%
TTTCTTCAGGATAACCCTAA+TGG + Chr8:52597356-52597375 MsG0880045001.01.T01:CDS 35.0%
TTTCTTCTGCTTGCAAATCT+TGG + Chr8:52596799-52596818 MsG0880045001.01.T01:CDS 35.0%
! TAATGGTTTGGAGGTTTACA+TGG + Chr8:52597373-52597392 MsG0880045001.01.T01:CDS 35.0%
! TGCATCTTTGCCTTCTTTTA+AGG + Chr8:52597643-52597662 MsG0880045001.01.T01:CDS 35.0%
!! GTGATGATGATGATACCTTT+TGG + Chr8:52597267-52597286 MsG0880045001.01.T01:CDS 35.0%
!! TGAATCGTCTTTGAAATTGG+TGG + Chr8:52597508-52597527 MsG0880045001.01.T01:CDS 35.0%
CTATACTGACCACTAACATC+AGG - Chr8:52597098-52597117 None:intergenic 40.0%
GAGACAATACACTTGCCAAA+AGG - Chr8:52597285-52597304 None:intergenic 40.0%
GATAGGTTGTATTTGCGTTC+TGG + Chr8:52596927-52596946 MsG0880045001.01.T01:CDS 40.0%
GTACCATTATTCTTGCCAGT+TGG - Chr8:52596714-52596733 None:intergenic 40.0%
TCAGGATAACCCTAATGGTT+TGG + Chr8:52597361-52597380 MsG0880045001.01.T01:CDS 40.0%
TGCAGCTGAAAGCTTAGAAT+TGG - Chr8:52597468-52597487 None:intergenic 40.0%
TGCTTAGCATCAAATGGAGA+TGG - Chr8:52596993-52597012 None:intergenic 40.0%
TTAAGGATCCTATCTCAGCT+GGG + Chr8:52597660-52597679 MsG0880045001.01.T01:CDS 40.0%
TTTAAGGATCCTATCTCAGC+TGG + Chr8:52597659-52597678 MsG0880045001.01.T01:CDS 40.0%
! ACATGGATGGTTTTTGCCAT+TGG + Chr8:52597390-52597409 MsG0880045001.01.T01:CDS 40.0%
!! ATTTTGGGTCAAATCGGTGT+CGG + Chr8:52597593-52597612 MsG0880045001.01.T01:CDS 40.0%
!! GATGGTTTTTGCCATTGGTT+GGG + Chr8:52597395-52597414 MsG0880045001.01.T01:CDS 40.0%
!! GTTTTGTCCACTTGTTAGTG+CGG - Chr8:52597307-52597326 None:intergenic 40.0%
!!! GTCGCTAATTTTCAGGTTCA+TGG + Chr8:52597170-52597189 MsG0880045001.01.T01:CDS 40.0%
AACTACCGGGATGATTGAGA+AGG + Chr8:52597745-52597764 MsG0880045001.01.T01:CDS 45.0%
ATTGTCTCCGCACTAACAAG+TGG + Chr8:52597297-52597316 MsG0880045001.01.T01:CDS 45.0%
CCACCAACTGGCAAGAATAA+TGG + Chr8:52596708-52596727 MsG0880045001.01.T01:CDS 45.0%
CCATTATTCTTGCCAGTTGG+TGG - Chr8:52596711-52596730 None:intergenic 45.0%
GGTTTGGAGGTTTACATGGA+TGG + Chr8:52597377-52597396 MsG0880045001.01.T01:CDS 45.0%
TTGTGGAACCCTGATGTTAG+TGG + Chr8:52597086-52597105 MsG0880045001.01.T01:CDS 45.0%
! GCGTTCTGGTGACAATTTTC+AGG + Chr8:52596941-52596960 MsG0880045001.01.T01:CDS 45.0%
! GGACTTCGTCGCTAATTTTC+AGG + Chr8:52597163-52597182 MsG0880045001.01.T01:CDS 45.0%
! GGATAACCCTAATGGTTTGG+AGG + Chr8:52597364-52597383 MsG0880045001.01.T01:CDS 45.0%
! GGATGGTTTTTGCCATTGGT+TGG + Chr8:52597394-52597413 MsG0880045001.01.T01:CDS 45.0%
!! AAATCGGTGTCGGAAATTCC+TGG + Chr8:52597603-52597622 MsG0880045001.01.T01:CDS 45.0%
CCTATCTCAGCTGGGAAGAA+GGG + Chr8:52597668-52597687 MsG0880045001.01.T01:CDS 50.0%
CTTTGACTCTGCCCAACCAA+TGG - Chr8:52597409-52597428 None:intergenic 50.0%
TCCTATCTCAGCTGGGAAGA+AGG + Chr8:52597667-52597686 MsG0880045001.01.T01:CDS 50.0%
ACTGACTATGCCGAGGACCT+AGG + Chr8:52597128-52597147 MsG0880045001.01.T01:CDS 55.0%
AGGTCCTCGGCATAGTCAGT+AGG - Chr8:52597128-52597147 None:intergenic 55.0%
AGTCCTCGTGTGGATACCCT+AGG - Chr8:52597148-52597167 None:intergenic 55.0%
CCCTTCTTCCCAGCTGAGAT+AGG - Chr8:52597671-52597690 None:intergenic 55.0%
CCTCGGCATAGTCAGTAGGA+AGG - Chr8:52597124-52597143 None:intergenic 55.0%
CTGACTATGCCGAGGACCTA+GGG + Chr8:52597129-52597148 MsG0880045001.01.T01:CDS 55.0%
GTGTGGATACCCTAGGTCCT+CGG - Chr8:52597141-52597160 None:intergenic 55.0%
TTAGCGACGAAGTCCTCGTG+TGG - Chr8:52597158-52597177 None:intergenic 55.0%
TTGGGCAGAGTCAAAGCACG+TGG + Chr8:52597413-52597432 MsG0880045001.01.T01:CDS 55.0%
! CCTTCCTACTGACTATGCCG+AGG + Chr8:52597121-52597140 MsG0880045001.01.T01:CDS 55.0%
! GGACCTAGGGTATCCACACG+AGG + Chr8:52597142-52597161 MsG0880045001.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 52596699 52597871 52596699 ID=MsG0880045001.01;Name=MsG0880045001.01
Chr8 mRNA 52596699 52597871 52596699 ID=MsG0880045001.01.T01;Parent=MsG0880045001.01;Name=MsG0880045001.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|390
Chr8 exon 52596699 52597871 52596699 ID=MsG0880045001.01.T01:exon:22539;Parent=MsG0880045001.01.T01
Chr8 CDS 52596699 52597871 52596699 ID=MsG0880045001.01.T01:cds;Parent=MsG0880045001.01.T01
Gene Sequence

>MsG0880045001.01.T01

ATGGCAAATCCACCAACTGGCAAGAATAATGGTACGTTTGTTACTGATGACATTGCTTTCTCTGTTCTCTCCAAACTTCCTCTTAAATCTATCAAGCGATTTTCTTCTGCTTGCAAATCTTGGTCTCAAGAGTTTGAAAAACCTAGTTTCTTACAAAAGTTCTTCAACAATCTTGTTTCCAAATCTCATGATGATGATGATGATGTGAAACCTCTCTTTAACATCAACGATAGGTTGTATTTGCGTTCTGGTGACAATTTTCAGGATGAAGAAGAATTGGTTATGAATCTGCCATCTCCATTTGATGCTAAGCATTTTCATATTTTAGGTTCTGCTATTCACGGTATTCTTTGTCTCTATATTGTTGACAATCAGAAGAATATAATCTTGTGGAACCCTGATGTTAGTGGTCAGTATAGAGTCCTTCCTACTGACTATGCCGAGGACCTAGGGTATCCACACGAGGACTTCGTCGCTAATTTTCAGGTTCATGGATTTGGTTATGACACTGTTAATCATGATTTCAAGGTCATTCAACTTGTGAATTATTATGGCTATAATGATTTAGGTGATGATGATGATACCTTTTGGCAAGTGTATTGTCTCCGCACTAACAAGTGGACAAAACTTAACATACCTAGTGGTCATAAGATTCCATTTCTTCAGGATAACCCTAATGGTTTGGAGGTTTACATGGATGGTTTTTGCCATTGGTTGGGCAGAGTCAAAGCACGTGGTCAACTTTATCTCGTGTCATTCAACTTGACCAATTCTAAGCTTTCAGCTGCACGTGTCAATGTAGATGTAACTGAATCGTCTTTGAAATTGGTGGTGTTAAATGGGTCTGTTGCTTTGATCACACAACATGATGATCCTATGTCTTTTAGCATTTCAATTTTGGGTCAAATCGGTGTCGGAAATTCCTGGACAAAACTTTTCAATGTTGCATCTTTGCCTTCTTTTAAGGATCCTATCTCAGCTGGGAAGAAGGGTGTTATATTCTTCAAAGGAGATAATAACAATGGCAGAGTAGCTTGTTTTGATTTAACTACCGGGATGATTGAGAAGGAAATTGATTTTAAAGAAGAAATAGATATTCAGCAAATCGTATTTTACAAGAAAAGTGACAAACTCCTTTCAATGAAGAGAAAGAAGAACACCGGTCGTCGTTGA
Protein sequence

>MsG0880045001.01.T01

MANPPTGKNNGTFVTDDIAFSVLSKLPLKSIKRFSSACKSWSQEFEKPSFLQKFFNNLVSKSHDDDDDVKPLFNINDRLYLRSGDNFQDEEELVMNLPSPFDAKHFHILGSAIHGILCLYIVDNQKNIILWNPDVSGQYRVLPTDYAEDLGYPHEDFVANFQVHGFGYDTVNHDFKVIQLVNYYGYNDLGDDDDTFWQVYCLRTNKWTKLNIPSGHKIPFLQDNPNGLEVYMDGFCHWLGRVKARGQLYLVSFNLTNSKLSAARVNVDVTESSLKLVVLNGSVALITQHDDPMSFSISILGQIGVGNSWTKLFNVASLPSFKDPISAGKKGVIFFKGDNNNGRVACFDLTTGMIEKEIDFKEEIDIQQIVFYKKSDKLLSMKRKKNTGRR*