AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045002.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045002.01.T01 MTR_8g063350 78.788 198 30 3 120 315 157 344 3.03e-102 303
MsG0880045002.01.T01 MTR_8g063350 64.348 115 7 1 1 115 1 81 4.01e-38 138
MsG0880045002.01.T01 MTR_8g063370 67.010 194 61 1 116 309 203 393 7.89e-85 261
MsG0880045002.01.T01 MTR_8g063370 50.420 119 53 4 1 115 11 127 1.23e-20 91.7
MsG0880045002.01.T01 MTR_8g063310 54.589 207 78 5 116 309 195 398 4.11e-64 208
MsG0880045002.01.T01 MTR_8g063310 49.167 120 53 3 1 115 9 125 5.22e-23 98.6
MsG0880045002.01.T01 MTR_8g036970 42.675 314 126 10 18 288 2 304 9.54e-64 206
MsG0880045002.01.T01 MTR_8g064790 39.118 340 149 12 4 299 3 328 8.63e-52 174
MsG0880045002.01.T01 MTR_4g014370 35.783 313 170 9 14 300 22 329 9.01e-51 171
MsG0880045002.01.T01 MTR_5g043605 36.391 327 156 14 11 301 22 332 9.18e-43 151
MsG0880045002.01.T01 MTR_8g064890 46.961 181 87 5 119 299 208 379 1.79e-42 151
MsG0880045002.01.T01 MTR_8g064890 49.425 87 39 1 8 89 10 96 7.31e-13 68.9
MsG0880045002.01.T01 MTR_7g079640 31.915 376 158 14 7 299 6 366 1.42e-39 143
MsG0880045002.01.T01 MTR_2g034700 44.086 186 94 4 119 304 197 372 1.91e-39 142
MsG0880045002.01.T01 MTR_2g034700 41.732 127 60 4 1 115 1 125 8.94e-18 83.2
MsG0880045002.01.T01 MTR_5g091140 30.311 386 182 14 6 310 40 419 3.91e-37 137
MsG0880045002.01.T01 MTR_0831s0010 28.022 364 156 9 9 280 45 394 3.60e-34 129
MsG0880045002.01.T01 MTR_1g050308 33.333 333 167 13 5 300 25 339 5.51e-34 127
MsG0880045002.01.T01 MTR_6g478230 30.829 386 167 13 8 300 21 399 9.47e-34 128
MsG0880045002.01.T01 MTR_8g063440 42.541 181 91 6 122 298 202 373 1.52e-33 127
MsG0880045002.01.T01 MTR_8g063440 42.342 111 53 3 14 115 12 120 3.90e-16 78.6
MsG0880045002.01.T01 MTR_4g028510 36.179 246 130 9 66 299 163 393 3.30e-33 127
MsG0880045002.01.T01 MTR_4g028510 34.810 158 81 7 8 153 10 157 3.27e-11 64.3
MsG0880045002.01.T01 MTR_6g090260 33.808 281 119 10 8 280 14 235 8.25e-33 122
MsG0880045002.01.T01 MTR_8g012090 29.141 326 161 8 14 276 16 334 1.29e-32 123
MsG0880045002.01.T01 MTR_0054s0090 39.037 187 101 4 119 299 218 397 1.78e-32 124
MsG0880045002.01.T01 MTR_6g086825 36.400 250 131 9 67 300 215 452 6.34e-32 124
MsG0880045002.01.T01 MTR_3g011120 42.246 187 92 7 115 297 195 369 6.96e-32 122
MsG0880045002.01.T01 MTR_3g011120 47.748 111 50 2 13 115 13 123 2.85e-20 90.5
MsG0880045002.01.T01 MTR_2g021800 37.173 191 105 5 119 300 224 408 1.02e-31 122
MsG0880045002.01.T01 MTR_2g021800 37.500 128 59 3 2 111 5 129 3.12e-17 82.0
MsG0880045002.01.T01 MTR_4g130330 39.796 196 94 8 119 300 198 383 1.04e-31 122
MsG0880045002.01.T01 MTR_4g007940 39.326 178 79 4 121 280 149 315 1.92e-31 120
MsG0880045002.01.T01 MTR_5g097330 37.436 195 106 7 120 308 217 401 3.17e-31 121
MsG0880045002.01.T01 MTR_6g080680 37.968 187 103 4 119 299 207 386 5.28e-31 120
MsG0880045002.01.T01 MTR_7g069550 41.026 195 82 11 120 299 214 390 7.66e-31 120
MsG0880045002.01.T01 MTR_7g069550 37.168 113 62 1 1 104 1 113 5.13e-12 66.6
MsG0880045002.01.T01 MTR_2g064950 37.306 193 100 8 119 299 198 381 8.96e-31 120
MsG0880045002.01.T01 MTR_2g064950 37.037 108 57 3 14 112 10 115 3.13e-11 63.9
MsG0880045002.01.T01 MTR_4g028660 32.653 245 140 8 67 300 161 391 9.82e-31 119
MsG0880045002.01.T01 MTR_4g028660 35.811 148 78 6 8 146 10 149 8.77e-12 65.9
MsG0880045002.01.T01 MTR_4g027450 32.653 245 140 8 67 300 161 391 9.82e-31 119
MsG0880045002.01.T01 MTR_4g027450 35.811 148 78 6 8 146 10 149 8.77e-12 65.9
MsG0880045002.01.T01 MTR_0054s0100 37.433 187 104 4 119 299 220 399 2.62e-30 119
MsG0880045002.01.T01 MTR_5g096910 41.279 172 83 6 116 281 207 366 2.71e-30 118
MsG0880045002.01.T01 MTR_0129s0120 38.095 189 101 6 119 300 209 388 4.55e-30 118
MsG0880045002.01.T01 MTR_0129s0120 37.288 118 64 3 6 115 9 124 4.35e-13 69.7
MsG0880045002.01.T01 MTR_5g097220 37.118 229 121 9 87 308 194 406 7.19e-30 117
MsG0880045002.01.T01 MTR_5g097220 42.683 82 45 1 14 93 13 94 2.50e-11 64.3
MsG0880045002.01.T01 MTR_6g090180 32.922 243 147 5 67 300 195 430 8.65e-30 117
MsG0880045002.01.T01 MTR_6g090180 35.088 114 63 3 6 110 39 150 8.22e-11 62.8
MsG0880045002.01.T01 MTR_2g022000 35.751 193 109 5 119 302 223 409 1.32e-29 117
MsG0880045002.01.T01 MTR_2g022000 36.800 125 63 3 2 111 5 128 1.70e-15 77.0
MsG0880045002.01.T01 MTR_7g101200 33.465 254 139 12 28 269 1 236 1.42e-29 113
MsG0880045002.01.T01 MTR_6g078010 37.879 198 106 6 122 310 147 336 2.53e-29 115
MsG0880045002.01.T01 MTR_0129s0050 34.375 224 117 7 84 300 200 400 3.92e-29 115
MsG0880045002.01.T01 MTR_0129s0050 37.500 152 71 8 14 154 14 152 4.60e-14 72.8
MsG0880045002.01.T01 MTR_4g014370 37.158 183 112 2 118 300 222 401 4.71e-29 115
MsG0880045002.01.T01 MTR_4g014370 36.842 114 58 5 14 115 11 122 3.60e-13 70.1
MsG0880045002.01.T01 MTR_0129s0070 37.566 189 102 6 119 300 209 388 8.46e-29 114
MsG0880045002.01.T01 MTR_0129s0070 41.818 110 54 3 14 115 17 124 7.55e-15 75.1
MsG0880045002.01.T01 MTR_6g090250 36.318 201 106 5 115 300 223 416 8.58e-29 115
MsG0880045002.01.T01 MTR_5g068490 38.710 186 98 6 119 300 96 269 1.99e-28 111
MsG0880045002.01.T01 MTR_5g097350 37.173 191 106 8 120 309 209 386 4.03e-28 112
MsG0880045002.01.T01 MTR_5g074800 40.526 190 88 11 122 300 161 336 4.63e-28 111
MsG0880045002.01.T01 MTR_5g023970 37.195 164 92 3 121 279 10 167 5.05e-28 107
MsG0880045002.01.T01 MTR_8g467670 37.766 188 98 6 121 304 203 375 6.59e-28 112
MsG0880045002.01.T01 MTR_8g467670 40.496 121 59 3 6 115 6 124 6.14e-18 84.0
MsG0880045002.01.T01 MTR_4g028680 33.068 251 129 9 68 300 163 392 1.31e-27 111
MsG0880045002.01.T01 MTR_4g028680 32.258 186 107 6 8 184 10 185 7.18e-12 65.9
MsG0880045002.01.T01 MTR_4g028750 39.429 175 94 5 119 287 209 377 1.51e-27 111
MsG0880045002.01.T01 MTR_4g028750 41.905 105 53 3 8 106 10 112 3.12e-13 70.1
MsG0880045002.01.T01 MTR_8g464770 36.957 184 101 7 121 300 200 372 1.55e-27 110
MsG0880045002.01.T01 MTR_8g464770 38.182 110 60 2 14 115 12 121 2.25e-13 70.5
MsG0880045002.01.T01 MTR_2g066200 38.220 191 98 8 121 300 199 380 2.67e-27 110
MsG0880045002.01.T01 MTR_2g066200 43.363 113 51 3 14 115 12 122 1.98e-17 82.4
MsG0880045002.01.T01 MTR_5g097680 35.610 205 112 6 94 288 215 409 2.80e-27 110
MsG0880045002.01.T01 MTR_5g097680 40.404 99 49 3 11 101 14 110 2.83e-12 67.4
MsG0880045002.01.T01 MTR_6g079790 36.364 198 103 6 119 300 111 301 7.71e-27 107
MsG0880045002.01.T01 MTR_2g066170 37.433 187 103 6 121 300 199 378 9.72e-27 108
MsG0880045002.01.T01 MTR_2g066170 41.525 118 58 3 5 113 3 118 4.66e-17 81.3
MsG0880045002.01.T01 MTR_2g065840 38.378 185 100 6 122 300 205 381 1.21e-26 108
MsG0880045002.01.T01 MTR_2g065840 43.750 96 43 3 13 101 11 102 2.53e-13 70.5
MsG0880045002.01.T01 MTR_2g038680 39.181 171 88 6 117 281 224 384 2.04e-26 108
MsG0880045002.01.T01 MTR_5g037680 34.359 195 110 5 116 300 192 378 2.33e-26 108
MsG0880045002.01.T01 MTR_3g018750 36.041 197 113 6 121 308 47 239 2.86e-26 104
MsG0880045002.01.T01 MTR_7g017130 34.197 193 104 7 119 300 209 389 3.05e-26 107
MsG0880045002.01.T01 MTR_5g092860 33.473 239 138 10 59 287 125 352 3.68e-26 106
MsG0880045002.01.T01 MTR_7g017170 35.632 174 90 6 119 281 209 371 5.69e-26 107
MsG0880045002.01.T01 MTR_6g087620 34.975 203 108 7 111 296 83 278 8.85e-26 103
MsG0880045002.01.T01 MTR_5g086590 35.260 173 96 4 113 279 69 231 1.23e-25 102
MsG0880045002.01.T01 MTR_0134s0060 34.921 189 107 5 119 298 221 402 1.46e-25 105
MsG0880045002.01.T01 MTR_0134s0060 38.261 115 57 5 6 110 4 114 1.06e-11 65.5
MsG0880045002.01.T01 MTR_5g097850 38.824 170 79 6 126 281 62 220 1.77e-25 102
MsG0880045002.01.T01 MTR_8g033000 37.563 197 96 7 118 300 172 355 2.32e-25 104
MsG0880045002.01.T01 MTR_2g021770 34.921 189 105 7 122 300 219 399 2.68e-25 105
MsG0880045002.01.T01 MTR_2g021770 33.333 111 61 2 14 111 12 122 9.09e-13 68.9
MsG0880045002.01.T01 MTR_5g064700 36.410 195 100 7 109 288 165 350 7.46e-25 103
MsG0880045002.01.T01 MTR_2g065860 37.634 186 100 6 121 299 203 379 8.74e-25 103
MsG0880045002.01.T01 MTR_2g065860 41.237 97 46 3 13 102 11 103 6.42e-13 69.3
MsG0880045002.01.T01 MTR_2g021920 38.021 192 95 9 122 300 85 265 1.16e-24 100
MsG0880045002.01.T01 MTR_6g079810 34.054 185 107 5 122 298 276 453 1.34e-24 103
MsG0880045002.01.T01 MTR_5g092850 36.041 197 109 6 121 308 212 400 1.69e-24 102
MsG0880045002.01.T01 MTR_1g098180 35.052 194 94 6 119 300 185 358 1.79e-24 102
MsG0880045002.01.T01 MTR_1g098180 46.237 93 44 1 9 95 14 106 1.26e-15 77.0
MsG0880045002.01.T01 MTR_2g021950 36.458 192 98 8 122 300 209 389 2.37e-24 102
MsG0880045002.01.T01 MTR_5g059430 31.746 189 113 3 121 299 235 417 2.45e-24 102
MsG0880045002.01.T01 MTR_6g461660 38.690 168 86 7 119 280 217 373 4.35e-24 101
MsG0880045002.01.T01 MTR_6g079770 35.106 188 106 5 121 299 228 408 5.81e-24 101
MsG0880045002.01.T01 MTR_6g009350 40.397 151 76 6 119 261 44 188 6.57e-24 97.1
MsG0880045002.01.T01 MTR_7g104710 30.634 284 124 9 6 221 9 287 7.65e-24 99.8
MsG0880045002.01.T01 MTR_8g032980 37.255 204 108 8 110 300 217 413 1.41e-23 100
MsG0880045002.01.T01 MTR_8g032980 42.857 98 50 3 14 111 16 107 1.73e-12 67.8
MsG0880045002.01.T01 MTR_2g066150 40.645 155 82 5 152 300 202 352 1.71e-23 99.4
MsG0880045002.01.T01 MTR_2g066150 41.228 114 62 3 5 115 3 114 1.19e-17 82.8
MsG0880045002.01.T01 MTR_6g004450 35.263 190 106 7 117 300 83 261 3.39e-23 97.1
MsG0880045002.01.T01 MTR_1g007410 34.759 187 106 7 122 302 224 400 3.76e-23 99.0
MsG0880045002.01.T01 MTR_1g007410 39.091 110 54 3 14 112 22 129 1.32e-11 65.1
MsG0880045002.01.T01 MTR_2g104340 35.789 190 103 7 122 300 207 388 7.71e-23 98.2
MsG0880045002.01.T01 MTR_7g019250 35.859 198 79 7 104 280 239 409 8.99e-23 98.6
MsG0880045002.01.T01 MTR_1g007450 36.527 167 95 4 121 280 207 369 1.37e-22 97.4
MsG0880045002.01.T01 MTR_7g023800 37.662 154 87 3 152 299 152 302 1.46e-22 95.9
MsG0880045002.01.T01 MTR_5g022850 34.555 191 114 7 119 306 167 349 2.35e-22 96.3
MsG0880045002.01.T01 MTR_5g069390 33.146 178 102 5 113 280 220 390 2.84e-22 97.1
MsG0880045002.01.T01 MTR_8g063330 37.748 151 73 3 121 260 208 348 3.17e-22 97.1
MsG0880045002.01.T01 MTR_8g063330 36.364 110 60 1 13 112 13 122 7.62e-14 72.0
MsG0880045002.01.T01 MTR_7g111700 28.382 377 166 16 14 311 15 366 4.20e-22 95.5
MsG0880045002.01.T01 MTR_5g024260 35.821 201 96 8 115 297 204 389 4.91e-22 95.9
MsG0880045002.01.T01 MTR_5g024260 35.503 169 89 5 8 160 16 180 2.10e-15 76.6
MsG0880045002.01.T01 MTR_8g039500 35.294 187 97 8 106 276 168 346 5.42e-22 95.1
MsG0880045002.01.T01 MTR_8g039500 42.982 114 60 2 5 115 3 114 1.49e-19 88.2
MsG0880045002.01.T01 MTR_5g072060 44.928 138 60 5 155 281 214 346 5.82e-22 96.7
MsG0880045002.01.T01 MTR_7g017040 36.264 182 88 6 117 281 202 372 8.40e-22 95.1
MsG0880045002.01.T01 MTR_2g104280 35.961 203 95 8 122 306 216 401 1.10e-21 94.7
MsG0880045002.01.T01 MTR_8g041270 37.288 177 82 7 122 281 217 381 1.24e-21 94.7
MsG0880045002.01.T01 MTR_1g050312 39.053 169 73 7 121 281 123 269 1.56e-21 92.8
MsG0880045002.01.T01 MTR_4g016000 38.571 140 69 4 121 258 69 193 3.04e-21 90.5
MsG0880045002.01.T01 MTR_5g097540 29.954 217 126 6 67 266 29 236 4.26e-21 91.7
MsG0880045002.01.T01 MTR_8g013000 38.158 152 76 5 119 261 209 351 9.87e-21 92.0
MsG0880045002.01.T01 MTR_8g035690 32.941 255 124 14 67 300 67 295 3.61e-20 89.4
MsG0880045002.01.T01 MTR_5g076490 35.052 194 107 6 119 300 142 328 5.16e-20 89.4
MsG0880045002.01.T01 MTR_4g055820 34.211 152 86 3 119 260 211 358 1.66e-19 88.2
MsG0880045002.01.T01 MTR_4g055820 31.646 158 93 3 1 150 1 151 7.00e-15 74.7
MsG0880045002.01.T01 MTR_5g096630 32.222 180 100 6 119 281 214 388 2.56e-19 88.2
MsG0880045002.01.T01 MTR_7g017150 31.707 205 121 8 111 304 67 263 3.50e-19 85.9
MsG0880045002.01.T01 MTR_6g079730 40.909 132 69 3 175 300 14 142 9.63e-19 81.6
MsG0880045002.01.T01 MTR_5g096920 36.923 130 73 4 157 281 1 126 1.57e-18 81.3
MsG0880045002.01.T01 MTR_8g046200 33.854 192 105 7 117 300 237 414 1.65e-18 85.9
MsG0880045002.01.T01 MTR_8g046200 36.000 125 55 4 8 112 10 129 8.15e-12 65.9
MsG0880045002.01.T01 MTR_4g102410 36.416 173 95 7 119 281 257 424 1.70e-18 85.9
MsG0880045002.01.T01 MTR_3g030620 32.927 164 94 5 154 305 20 179 2.04e-18 81.6
MsG0880045002.01.T01 MTR_7g017120 31.863 204 121 8 111 304 67 262 2.81e-18 83.2
MsG0880045002.01.T01 MTR_1g053510 32.979 188 106 7 119 300 225 398 3.35e-18 84.7
MsG0880045002.01.T01 MTR_1g053510 38.211 123 63 4 1 112 1 121 8.73e-14 71.6
MsG0880045002.01.T01 MTR_5g074850 32.524 206 121 10 121 315 234 432 7.04e-18 84.7
MsG0880045002.01.T01 MTR_2g022020 41.905 105 48 3 200 300 17 112 1.26e-17 77.8
MsG0880045002.01.T01 MTR_8g046310 29.592 196 122 7 119 310 195 378 1.08e-16 80.1
MsG0880045002.01.T01 MTR_8g046310 40.909 110 54 2 14 112 16 125 1.66e-14 73.9
MsG0880045002.01.T01 MTR_4g117550 31.285 179 96 6 122 300 214 365 1.49e-16 79.7
MsG0880045002.01.T01 MTR_4g117550 34.513 113 61 3 14 115 17 127 7.99e-12 65.9
MsG0880045002.01.T01 MTR_5g066420 36.296 135 73 3 157 280 1 133 3.58e-16 74.7
MsG0880045002.01.T01 MTR_8g046220 32.143 168 98 6 119 281 224 380 1.17e-15 77.4
MsG0880045002.01.T01 MTR_8g046220 43.956 91 45 1 14 98 17 107 5.09e-14 72.4
MsG0880045002.01.T01 MTR_8g018110 30.769 208 122 8 102 307 207 394 1.53e-15 77.0
MsG0880045002.01.T01 MTR_8g018110 36.937 111 56 5 12 112 12 118 7.98e-11 62.8
MsG0880045002.01.T01 MTR_5g097660 48.889 90 41 3 193 280 7 93 5.51e-15 70.9
MsG0880045002.01.T01 MTR_5g075370 31.953 169 99 8 121 279 210 372 7.30e-15 75.5
MsG0880045002.01.T01 MTR_8g033110 31.220 205 112 8 118 307 222 412 1.79e-14 73.9
MsG0880045002.01.T01 MTR_8g033110 33.613 119 72 1 1 112 1 119 3.03e-11 64.3
MsG0880045002.01.T01 MTR_8g033090 32.258 217 125 9 101 307 205 409 2.10e-14 73.6
MsG0880045002.01.T01 MTR_8g033090 36.036 111 59 3 12 115 12 117 2.05e-11 64.7
MsG0880045002.01.T01 MTR_5g097650 39.423 104 59 2 13 115 21 121 2.58e-14 70.1
MsG0880045002.01.T01 MTR_5g098950 31.677 161 71 6 111 261 141 272 6.58e-14 71.2
MsG0880045002.01.T01 MTR_7g016930 36.719 128 64 5 117 236 186 304 7.34e-14 71.6
MsG0880045002.01.T01 MTR_1g007430 27.984 243 128 7 31 258 109 319 9.37e-14 71.2
MsG0880045002.01.T01 MTR_3g047580 37.398 123 47 4 1 115 1 101 8.66e-13 64.7
MsG0880045002.01.T01 MTR_3g019290 33.333 132 71 3 1 115 1 132 8.75e-13 66.2
MsG0880045002.01.T01 MTR_8g033140 31.282 195 98 8 119 300 239 410 9.72e-13 68.6
MsG0880045002.01.T01 MTR_8g033140 35.644 101 55 2 9 101 14 112 4.27e-11 63.9
MsG0880045002.01.T01 MTR_5g036250 35.965 114 70 2 194 307 1 111 1.07e-12 65.9
MsG0880045002.01.T01 MTR_8g073570 36.752 117 65 3 6 115 2 116 1.86e-12 65.1
MsG0880045002.01.T01 MTR_1g050305 49.383 81 34 3 206 281 21 99 1.96e-12 64.3
MsG0880045002.01.T01 MTR_5g094350 57.143 49 21 0 11 59 10 58 1.99e-11 63.9
MsG0880045002.01.T01 MTR_5g086780 65.854 41 14 0 14 54 16 56 4.93e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 49 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAAATTGGTGGTGTTAAA+TGG 0.199974 8:+52600212 MsG0880045002.01.T01:CDS
GATAGATTTAAGAGGAAGTT+TGG 0.264442 8:-52599465 None:intergenic
TTTCTTCTGCTTGCAAATCT+TGG 0.315310 8:+52599495 MsG0880045002.01.T01:CDS
ATCATCATCATCATGAGATT+TGG 0.319862 8:-52599573 None:intergenic
AACTGAATCGTCTTTGAAAT+TGG 0.323062 8:+52600199 MsG0880045002.01.T01:CDS
AAGTAAACCTCCAAACCATT+AGG 0.328295 8:-52600065 None:intergenic
TCATCAGATTCCATTTCTTC+AGG 0.339382 8:+52600037 MsG0880045002.01.T01:CDS
GATAGGTTGTATTTGCGTTC+TGG 0.359347 8:+52599623 MsG0880045002.01.T01:CDS
TCAGGATAACCCTAATGGTT+TGG 0.364228 8:+52600055 MsG0880045002.01.T01:CDS
TAATGGTTTGGAGGTTTACT+TGG 0.369383 8:+52600067 MsG0880045002.01.T01:CDS
TTTCTTCAGGATAACCCTAA+TGG 0.382039 8:+52600050 MsG0880045002.01.T01:CDS
CCACCAACTGGCAAGAATAA+TGG 0.384394 8:+52599404 MsG0880045002.01.T01:CDS
TTTAAGGATCCTATCTCAGC+TGG 0.407616 8:+52600353 MsG0880045002.01.T01:CDS
CCCTTCTTCCCAGCTGAGAT+AGG 0.413249 8:-52600362 None:intergenic
AATCGCTTGATAGATTTAAG+AGG 0.421126 8:-52599473 None:intergenic
TTAGGGTTATCCTGAAGAAA+TGG 0.422985 8:-52600047 None:intergenic
AGTAAACCTCCAAACCATTA+GGG 0.423080 8:-52600064 None:intergenic
GGTTTGGAGGTTTACTTGGA+TGG 0.426529 8:+52600071 MsG0880045002.01.T01:CDS
CCATTATTCTTGCCAGTTGG+TGG 0.426616 8:-52599404 None:intergenic
TGCAGCTGAAAGCTTAGAAT+TGG 0.431001 8:-52600159 None:intergenic
TGAAAATGCTTAGCATCAAA+TGG 0.432594 8:-52599692 None:intergenic
AAATCGGTGTCGGAAATTCC+TGG 0.436340 8:+52600297 MsG0880045002.01.T01:CDS
GTGATGATGATGATACCTTT+TGG 0.437422 8:+52599961 MsG0880045002.01.T01:CDS
TCCTATCTCAGCTGGGAAGA+AGG 0.438071 8:+52600361 MsG0880045002.01.T01:CDS
TGAAATTGGTGGTGTTAAAT+GGG 0.458418 8:+52600213 MsG0880045002.01.T01:CDS
TCTTCTTTCTCTTCATTGAA+AGG 0.458533 8:-52600526 None:intergenic
ACCTTCAGCTCAACGACGAC+CGG 0.490270 8:-52600552 None:intergenic
GGATAACCCTAATGGTTTGG+AGG 0.504009 8:+52600058 MsG0880045002.01.T01:CDS
TCAATGGCAAATCCACCAAC+TGG 0.512413 8:+52599392 None:intergenic
GAGATAGGATCCTTAAAAGA+AGG 0.529126 8:-52600347 None:intergenic
CCTATCTCAGCTGGGAAGAA+GGG 0.535589 8:+52600362 MsG0880045002.01.T01:CDS
AAGGGTGTTATATTCTTCAA+AGG 0.535738 8:+52600380 MsG0880045002.01.T01:CDS
ATTGAAATGCTAAAAGACAT+AGG 0.542826 8:-52600266 None:intergenic
CTTTGACTCTGCCCAACCAA+TGG 0.561403 8:-52600100 None:intergenic
GAGACAATACACTTGCCAAA+AGG 0.561919 8:-52599976 None:intergenic
TGCTTAGCATCAAATGGAGA+TGG 0.578406 8:-52599686 None:intergenic
CTCTCTTTAACATCAACGAT+AGG 0.586154 8:+52599606 MsG0880045002.01.T01:CDS
TGAATCGTCTTTGAAATTGG+TGG 0.586889 8:+52600202 MsG0880045002.01.T01:CDS
AATGGAATCTGATGACCACT+AGG 0.590233 8:-52600029 None:intergenic
AACTACCGGGATGATTGAGA+AGG 0.593822 8:+52600439 MsG0880045002.01.T01:CDS
GTACCATTATTCTTGCCAGT+TGG 0.595191 8:-52599407 None:intergenic
AATTTCCTTCTCAATCATCC+CGG 0.600741 8:-52600444 None:intergenic
TTCAAAGGAGATAATAACAA+TGG 0.603159 8:+52600395 MsG0880045002.01.T01:CDS
TTAAGGATCCTATCTCAGCT+GGG 0.604146 8:+52600354 MsG0880045002.01.T01:CDS
ATTGTCTCCGCACTAACAAG+TGG 0.607609 8:+52599991 MsG0880045002.01.T01:CDS
CTATCGTTGATGTTAAAGAG+AGG 0.627836 8:-52599605 None:intergenic
ATGAAGAGAAAGAAGAACAC+CGG 0.665888 8:+52600533 MsG0880045002.01.T01:CDS
ACAAAACTTAACATACCTAG+TGG 0.685183 8:+52600014 MsG0880045002.01.T01:CDS
TTGGGCAGAGTCAAAGCACG+TGG 0.724731 8:+52600107 MsG0880045002.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCAGAAAATATAATCTTG+TGG + Chr8:52599764-52599783 MsG0880045002.01.T01:intron 20.0%
!! ATTCAACTTGTGAATTATTA+TGG + Chr8:52599924-52599943 MsG0880045002.01.T01:intron 20.0%
!! TATTATGGCTATAATGATTT+AGG + Chr8:52599939-52599958 MsG0880045002.01.T01:intron 20.0%
!!! TCTTTTAGCATTTCAATTTT+GGG + Chr8:52600272-52600291 MsG0880045002.01.T01:CDS 20.0%
! ATTGAAATGCTAAAAGACAT+AGG - Chr8:52600269-52600288 None:intergenic 25.0%
! TTCAAAGGAGATAATAACAA+TGG + Chr8:52600395-52600414 MsG0880045002.01.T01:CDS 25.0%
!! AAGAACTTTTGTAAGAAACT+AGG - Chr8:52599539-52599558 None:intergenic 25.0%
!! GCTAAGCATTTTCATATTTT+AGG + Chr8:52599701-52599720 MsG0880045002.01.T01:CDS 25.0%
!! GTCTTTTAGCATTTCAATTT+TGG + Chr8:52600271-52600290 MsG0880045002.01.T01:CDS 25.0%
!!! ATTTCAATTTTGGGTCAAAT+CGG + Chr8:52600281-52600300 MsG0880045002.01.T01:CDS 25.0%
AATCGCTTGATAGATTTAAG+AGG - Chr8:52599476-52599495 None:intergenic 30.0%
ACAAAACTTAACATACCTAG+TGG + Chr8:52600014-52600033 MsG0880045002.01.T01:CDS 30.0%
ATCATCATCATCATGAGATT+TGG - Chr8:52599576-52599595 None:intergenic 30.0%
CACTGTTAATCATGATTTCA+AGG + Chr8:52599899-52599918 MsG0880045002.01.T01:intron 30.0%
GATAGATTTAAGAGGAAGTT+TGG - Chr8:52599468-52599487 None:intergenic 30.0%
TCTTCTTTCTCTTCATTGAA+AGG - Chr8:52600529-52600548 None:intergenic 30.0%
TGAAAATGCTTAGCATCAAA+TGG - Chr8:52599695-52599714 None:intergenic 30.0%
!! AACATTGAAAAGTTTTGTCC+AGG - Chr8:52600318-52600337 None:intergenic 30.0%
!! AACTGAATCGTCTTTGAAAT+TGG + Chr8:52600199-52600218 MsG0880045002.01.T01:CDS 30.0%
!! AAGGGTGTTATATTCTTCAA+AGG + Chr8:52600380-52600399 MsG0880045002.01.T01:CDS 30.0%
!! TGAAATTGGTGGTGTTAAAT+GGG + Chr8:52600213-52600232 MsG0880045002.01.T01:CDS 30.0%
!! TTGAAATTGGTGGTGTTAAA+TGG + Chr8:52600212-52600231 MsG0880045002.01.T01:CDS 30.0%
!! TTTTCAGGATGAAGAAGAAT+TGG + Chr8:52599652-52599671 MsG0880045002.01.T01:CDS 30.0%
!!! AATTTTCAGGTTCATGGATT+TGG + Chr8:52599870-52599889 MsG0880045002.01.T01:intron 30.0%
!!! ATTTTAGGTTCTGCTATTCA+CGG + Chr8:52599716-52599735 MsG0880045002.01.T01:CDS 30.0%
!!! CTTGTTTTGATTTAACTACC+GGG + Chr8:52600426-52600445 MsG0880045002.01.T01:CDS 30.0%
!!! GCTTGTTTTGATTTAACTAC+CGG + Chr8:52600425-52600444 MsG0880045002.01.T01:CDS 30.0%
AAGTAAACCTCCAAACCATT+AGG - Chr8:52600068-52600087 None:intergenic 35.0%
AATTTCCTTCTCAATCATCC+CGG - Chr8:52600447-52600466 None:intergenic 35.0%
AGTAAACCTCCAAACCATTA+GGG - Chr8:52600067-52600086 None:intergenic 35.0%
ATGAAGAGAAAGAAGAACAC+CGG + Chr8:52600533-52600552 MsG0880045002.01.T01:CDS 35.0%
CTATCGTTGATGTTAAAGAG+AGG - Chr8:52599608-52599627 None:intergenic 35.0%
CTCTCTTTAACATCAACGAT+AGG + Chr8:52599606-52599625 MsG0880045002.01.T01:CDS 35.0%
GAGATAGGATCCTTAAAAGA+AGG - Chr8:52600350-52600369 None:intergenic 35.0%
TATACTGACCACTAACATCA+GGG - Chr8:52599792-52599811 None:intergenic 35.0%
TCATCAGATTCCATTTCTTC+AGG + Chr8:52600037-52600056 MsG0880045002.01.T01:CDS 35.0%
TTAGGGTTATCCTGAAGAAA+TGG - Chr8:52600050-52600069 None:intergenic 35.0%
TTTCTTCAGGATAACCCTAA+TGG + Chr8:52600050-52600069 MsG0880045002.01.T01:CDS 35.0%
TTTCTTCTGCTTGCAAATCT+TGG + Chr8:52599495-52599514 MsG0880045002.01.T01:CDS 35.0%
! AGACTTCGTCTCTAATTTTC+AGG + Chr8:52599857-52599876 MsG0880045002.01.T01:intron 35.0%
! TAATGGTTTGGAGGTTTACT+TGG + Chr8:52600067-52600086 MsG0880045002.01.T01:CDS 35.0%
! TGCATCTTTGCCTTCTTTTA+AGG + Chr8:52600337-52600356 MsG0880045002.01.T01:CDS 35.0%
!! GTGATGATGATGATACCTTT+TGG + Chr8:52599961-52599980 MsG0880045002.01.T01:CDS 35.0%
!! TGAATCGTCTTTGAAATTGG+TGG + Chr8:52600202-52600221 MsG0880045002.01.T01:CDS 35.0%
!!! GTCTCTAATTTTCAGGTTCA+TGG + Chr8:52599864-52599883 MsG0880045002.01.T01:intron 35.0%
AATGGAATCTGATGACCACT+AGG - Chr8:52600032-52600051 None:intergenic 40.0%
CTATACTGACCACTAACATC+AGG - Chr8:52599793-52599812 None:intergenic 40.0%
GAGACAATACACTTGCCAAA+AGG - Chr8:52599979-52599998 None:intergenic 40.0%
GATAGGTTGTATTTGCGTTC+TGG + Chr8:52599623-52599642 MsG0880045002.01.T01:CDS 40.0%
GTACCATTATTCTTGCCAGT+TGG - Chr8:52599410-52599429 None:intergenic 40.0%
TCAGGATAACCCTAATGGTT+TGG + Chr8:52600055-52600074 MsG0880045002.01.T01:CDS 40.0%
TGCAGCTGAAAGCTTAGAAT+TGG - Chr8:52600162-52600181 None:intergenic 40.0%
TGCTTAGCATCAAATGGAGA+TGG - Chr8:52599689-52599708 None:intergenic 40.0%
TTAAGGATCCTATCTCAGCT+GGG + Chr8:52600354-52600373 MsG0880045002.01.T01:CDS 40.0%
TTTAAGGATCCTATCTCAGC+TGG + Chr8:52600353-52600372 MsG0880045002.01.T01:CDS 40.0%
! ACTTGGATGGTTTTTGCCAT+TGG + Chr8:52600084-52600103 MsG0880045002.01.T01:CDS 40.0%
!! ATTTTGGGTCAAATCGGTGT+CGG + Chr8:52600287-52600306 MsG0880045002.01.T01:CDS 40.0%
!! GATGGTTTTTGCCATTGGTT+GGG + Chr8:52600089-52600108 MsG0880045002.01.T01:CDS 40.0%
!! GTTTTGTCCACTTGTTAGTG+CGG - Chr8:52600001-52600020 None:intergenic 40.0%
AACTACCGGGATGATTGAGA+AGG + Chr8:52600439-52600458 MsG0880045002.01.T01:CDS 45.0%
ATTAGAGACGAAGTCTCGTG+TGG - Chr8:52599853-52599872 None:intergenic 45.0%
ATTGTCTCCGCACTAACAAG+TGG + Chr8:52599991-52600010 MsG0880045002.01.T01:CDS 45.0%
CCACCAACTGGCAAGAATAA+TGG + Chr8:52599404-52599423 MsG0880045002.01.T01:CDS 45.0%
CCATTATTCTTGCCAGTTGG+TGG - Chr8:52599407-52599426 None:intergenic 45.0%
GGTTTGGAGGTTTACTTGGA+TGG + Chr8:52600071-52600090 MsG0880045002.01.T01:CDS 45.0%
TTGTGGAACCCTGATGTTAG+TGG + Chr8:52599781-52599800 MsG0880045002.01.T01:intron 45.0%
! GCGTTCTGGTGACAATTTTC+AGG + Chr8:52599637-52599656 MsG0880045002.01.T01:CDS 45.0%
! GGATAACCCTAATGGTTTGG+AGG + Chr8:52600058-52600077 MsG0880045002.01.T01:CDS 45.0%
! GGATGGTTTTTGCCATTGGT+TGG + Chr8:52600088-52600107 MsG0880045002.01.T01:CDS 45.0%
!! AAATCGGTGTCGGAAATTCC+TGG + Chr8:52600297-52600316 MsG0880045002.01.T01:CDS 45.0%
AAGTCTCGTGTGGATACCCT+AGG - Chr8:52599843-52599862 None:intergenic 50.0%
CCTATCTCAGCTGGGAAGAA+GGG + Chr8:52600362-52600381 MsG0880045002.01.T01:CDS 50.0%
CTTTGACTCTGCCCAACCAA+TGG - Chr8:52600103-52600122 None:intergenic 50.0%
TCCTATCTCAGCTGGGAAGA+AGG + Chr8:52600361-52600380 MsG0880045002.01.T01:CDS 50.0%
ACTGACTATGCCGAGGACCT+AGG + Chr8:52599823-52599842 MsG0880045002.01.T01:intron 55.0%
AGGTCCTCGGCATAGTCAGT+AGG - Chr8:52599823-52599842 None:intergenic 55.0%
CCCTTCTTCCCAGCTGAGAT+AGG - Chr8:52600365-52600384 None:intergenic 55.0%
CCTCGGCATAGTCAGTAGGA+AGG - Chr8:52599819-52599838 None:intergenic 55.0%
CTGACTATGCCGAGGACCTA+GGG + Chr8:52599824-52599843 MsG0880045002.01.T01:intron 55.0%
GTGTGGATACCCTAGGTCCT+CGG - Chr8:52599836-52599855 None:intergenic 55.0%
TTGGGCAGAGTCAAAGCACG+TGG + Chr8:52600107-52600126 MsG0880045002.01.T01:CDS 55.0%
! CCTTCCTACTGACTATGCCG+AGG + Chr8:52599816-52599835 MsG0880045002.01.T01:intron 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 52599395 52600565 52599395 ID=MsG0880045002.01;Name=MsG0880045002.01
Chr8 mRNA 52599395 52600565 52599395 ID=MsG0880045002.01.T01;Parent=MsG0880045002.01;Name=MsG0880045002.01.T01;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|1|1|2|0|315
Chr8 exon 52599395 52599737 52599395 ID=MsG0880045002.01.T01:exon:22540;Parent=MsG0880045002.01.T01
Chr8 exon 52599961 52600565 52599961 ID=MsG0880045002.01.T01:exon:22541;Parent=MsG0880045002.01.T01
Chr8 CDS 52599395 52599737 52599395 ID=MsG0880045002.01.T01:cds;Parent=MsG0880045002.01.T01
Chr8 CDS 52599961 52600565 52599961 ID=MsG0880045002.01.T01:cds;Parent=MsG0880045002.01.T01
Gene Sequence

>MsG0880045002.01.T01

ATGGCAAATCCACCAACTGGCAAGAATAATGGTACGTTTGTTACTGATGACATTGCTTTCTCTGTTCTCTCCAAACTTCCTCTTAAATCTATCAAGCGATTTTCTTCTGCTTGCAAATCTTGGTCTCAAGAGTTTGAAAAACCTAGTTTCTTACAAAAGTTCTTCAACAATCTTGTTTCCAAATCTCATGATGATGATGATGATGTGAAACCTCTCTTTAACATCAACGATAGGTTGTATTTGCGTTCTGGTGACAATTTTCAGGATGAAGAAGAATTGGTTATGAATCTGCCATCTCCATTTGATGCTAAGCATTTTCATATTTTAGGTTCTGCTATTCACGGTGATGATGATGATACCTTTTGGCAAGTGTATTGTCTCCGCACTAACAAGTGGACAAAACTTAACATACCTAGTGGTCATCAGATTCCATTTCTTCAGGATAACCCTAATGGTTTGGAGGTTTACTTGGATGGTTTTTGCCATTGGTTGGGCAGAGTCAAAGCACGTGGTCAACTTTATCTCGTGTCATTCAACTTGACCAATTCTAAGCTTTCAGCTGCACGTGTCAATGTAGATGTAACTGAATCGTCTTTGAAATTGGTGGTGTTAAATGGGTCTGTTGCTTTGATCACACAACATGATGATCCTATGTCTTTTAGCATTTCAATTTTGGGTCAAATCGGTGTCGGAAATTCCTGGACAAAACTTTTCAATGTTGCATCTTTGCCTTCTTTTAAGGATCCTATCTCAGCTGGGAAGAAGGGTGTTATATTCTTCAAAGGAGATAATAACAATGGCAGAGTAGCTTGTTTTGATTTAACTACCGGGATGATTGAGAAGGAAATTGATTTTAAAGAAGAAATAGATATTCAGCAAATCGTATTTTACAAGAAAAGTGACAAACTCCTTTCAATGAAGAGAAAGAAGAACACCGGTCGTCGTTGA
Protein sequence

>MsG0880045002.01.T01

MANPPTGKNNGTFVTDDIAFSVLSKLPLKSIKRFSSACKSWSQEFEKPSFLQKFFNNLVSKSHDDDDDVKPLFNINDRLYLRSGDNFQDEEELVMNLPSPFDAKHFHILGSAIHGDDDDTFWQVYCLRTNKWTKLNIPSGHQIPFLQDNPNGLEVYLDGFCHWLGRVKARGQLYLVSFNLTNSKLSAARVNVDVTESSLKLVVLNGSVALITQHDDPMSFSISILGQIGVGNSWTKLFNVASLPSFKDPISAGKKGVIFFKGDNNNGRVACFDLTTGMIEKEIDFKEEIDIQQIVFYKKSDKLLSMKRKKNTGRR*