AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680034923.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034923.01.T01 MTR_6g078010 71.731 283 47 2 54 336 1 250 9.57e-143 408
MsG0680034923.01.T01 MTR_6g079770 56.156 333 140 3 1 332 1 328 5.94e-120 352
MsG0680034923.01.T01 MTR_0134s0060 55.183 328 143 2 9 336 4 327 1.87e-119 351
MsG0680034923.01.T01 MTR_0054s0100 52.047 342 139 5 1 336 1 323 3.65e-115 340
MsG0680034923.01.T01 MTR_0054s0090 52.047 342 137 5 1 336 1 321 5.46e-115 339
MsG0680034923.01.T01 MTR_6g079810 55.394 343 128 10 1 336 54 378 2.58e-114 340
MsG0680034923.01.T01 MTR_7g104710 53.429 350 99 6 1 343 1 293 3.79e-112 329
MsG0680034923.01.T01 MTR_4g028510 52.711 332 135 7 3 332 2 313 1.23e-110 330
MsG0680034923.01.T01 MTR_6g086825 54.167 336 123 9 10 336 62 375 8.40e-105 315
MsG0680034923.01.T01 MTR_6g090250 54.206 321 118 8 35 336 29 339 2.03e-103 310
MsG0680034923.01.T01 MTR_6g080680 52.212 339 130 6 1 336 1 310 1.35e-102 307
MsG0680034923.01.T01 MTR_4g130330 51.863 322 128 8 14 332 4 301 4.29e-101 303
MsG0680034923.01.T01 MTR_4g028680 51.964 331 135 9 3 331 2 310 1.66e-100 302
MsG0680034923.01.T01 MTR_4g028660 49.547 331 144 7 3 332 2 310 8.50e-100 300
MsG0680034923.01.T01 MTR_4g027450 49.547 331 144 7 3 332 2 310 8.50e-100 300
MsG0680034923.01.T01 MTR_4g028750 52.727 330 129 10 5 331 3 308 5.22e-98 296
MsG0680034923.01.T01 MTR_6g090180 49.853 341 148 11 1 336 31 353 3.33e-95 290
MsG0680034923.01.T01 MTR_6g478230 49.118 340 115 9 14 336 24 322 3.21e-87 269
MsG0680034923.01.T01 MTR_6g070880 55.556 252 103 4 2 253 17 259 8.80e-87 262
MsG0680034923.01.T01 MTR_6g071420 55.556 252 103 4 2 253 17 259 8.80e-87 262
MsG0680034923.01.T01 MTR_8g063440 44.643 336 143 12 6 336 1 298 2.33e-73 232
MsG0680034923.01.T01 MTR_6g465360 55.610 205 89 1 2 206 47 249 3.98e-72 226
MsG0680034923.01.T01 MTR_0129s0050 41.298 339 167 8 1 329 1 317 1.93e-69 223
MsG0680034923.01.T01 MTR_5g097220 39.706 340 182 7 1 336 1 321 8.84e-69 221
MsG0680034923.01.T01 MTR_1g007410 40.882 340 159 12 3 331 8 316 3.60e-68 219
MsG0680034923.01.T01 MTR_0129s0070 43.713 334 151 11 1 328 1 303 3.92e-68 219
MsG0680034923.01.T01 MTR_2g038680 41.880 351 163 14 3 334 2 330 2.43e-66 215
MsG0680034923.01.T01 MTR_6g079790 52.263 243 89 8 101 336 2 224 2.82e-66 211
MsG0680034923.01.T01 MTR_4g117550 41.570 344 157 13 1 336 1 308 5.51e-66 213
MsG0680034923.01.T01 MTR_0129s0120 43.713 334 151 11 1 328 1 303 4.10e-65 211
MsG0680034923.01.T01 MTR_6g461660 40.988 344 157 11 2 333 4 313 8.47e-65 211
MsG0680034923.01.T01 MTR_2g022000 39.826 344 173 10 1 329 1 325 9.45e-65 211
MsG0680034923.01.T01 MTR_6g087620 47.809 251 73 7 90 330 2 204 2.29e-63 203
MsG0680034923.01.T01 MTR_8g012090 39.403 335 130 8 3 328 2 272 3.93e-63 204
MsG0680034923.01.T01 MTR_7g023800 42.391 276 104 3 56 331 1 221 8.78e-63 202
MsG0680034923.01.T01 MTR_7g017130 41.194 335 162 13 3 332 4 308 9.72e-63 205
MsG0680034923.01.T01 MTR_7g017170 41.194 335 162 13 3 332 4 308 1.05e-62 205
MsG0680034923.01.T01 MTR_2g021800 39.130 345 175 10 1 329 1 326 1.73e-61 202
MsG0680034923.01.T01 MTR_5g097680 39.106 358 175 12 1 332 1 341 1.22e-60 200
MsG0680034923.01.T01 MTR_5g096630 41.003 339 169 12 5 336 6 320 1.37e-60 200
MsG0680034923.01.T01 MTR_4g102410 41.108 343 168 14 1 334 44 361 1.94e-60 201
MsG0680034923.01.T01 MTR_5g097330 41.210 347 149 14 6 330 5 318 2.11e-60 199
MsG0680034923.01.T01 MTR_4g055820 39.403 335 166 11 7 330 4 312 1.22e-59 196
MsG0680034923.01.T01 MTR_5g037680 41.994 331 126 14 14 326 5 287 1.71e-59 198
MsG0680034923.01.T01 MTR_5g092850 40.950 337 157 13 4 329 2 307 6.30e-59 195
MsG0680034923.01.T01 MTR_8g039500 39.506 324 148 13 6 326 1 279 8.16e-59 193
MsG0680034923.01.T01 MTR_2g021950 38.372 344 160 16 1 329 1 307 2.78e-58 193
MsG0680034923.01.T01 MTR_5g074850 40.950 337 159 15 5 329 22 330 7.00e-58 201
MsG0680034923.01.T01 MTR_2g021770 38.192 343 169 14 11 336 6 322 3.78e-57 191
MsG0680034923.01.T01 MTR_7g069550 37.758 339 163 13 7 330 4 309 3.97e-57 190
MsG0680034923.01.T01 MTR_2g066170 38.838 327 155 13 6 326 1 288 1.90e-56 188
MsG0680034923.01.T01 MTR_2g065860 39.053 338 162 12 6 336 1 301 2.99e-56 188
MsG0680034923.01.T01 MTR_8g064890 39.009 323 152 12 15 330 14 298 4.25e-56 187
MsG0680034923.01.T01 MTR_2g034700 39.645 338 143 11 9 337 5 290 8.48e-56 186
MsG0680034923.01.T01 MTR_7g017040 39.193 347 167 11 6 342 5 317 9.02e-56 187
MsG0680034923.01.T01 MTR_5g024260 40.184 326 151 11 14 332 19 307 1.96e-55 186
MsG0680034923.01.T01 MTR_5g075370 40.367 327 159 14 14 329 5 306 2.25e-55 193
MsG0680034923.01.T01 MTR_2g065840 37.278 338 167 12 6 336 1 300 2.51e-55 186
MsG0680034923.01.T01 MTR_5g097350 40.351 342 144 14 1 328 1 296 3.34e-55 185
MsG0680034923.01.T01 MTR_3g011120 39.157 332 152 15 7 332 4 291 5.21e-55 184
MsG0680034923.01.T01 MTR_8g464770 38.462 338 158 13 6 336 1 295 9.48e-55 184
MsG0680034923.01.T01 MTR_1g007450 36.391 338 181 12 6 336 1 311 1.41e-53 182
MsG0680034923.01.T01 MTR_2g066200 37.313 335 163 11 6 332 1 296 1.84e-53 181
MsG0680034923.01.T01 MTR_2g104340 37.791 344 175 13 7 343 4 315 8.14e-53 179
MsG0680034923.01.T01 MTR_8g041270 37.685 337 159 13 13 330 11 315 2.38e-52 178
MsG0680034923.01.T01 MTR_8g467670 35.976 328 159 12 15 336 12 294 5.03e-52 177
MsG0680034923.01.T01 MTR_7g079640 37.576 330 145 12 16 336 12 289 2.69e-51 175
MsG0680034923.01.T01 MTR_7g016930 39.210 329 150 13 1 328 1 280 9.30e-51 172
MsG0680034923.01.T01 MTR_0831s0010 35.942 345 172 12 1 336 32 336 2.16e-50 173
MsG0680034923.01.T01 MTR_5g069390 37.462 331 156 13 15 326 24 322 3.98e-50 174
MsG0680034923.01.T01 MTR_2g104280 38.053 339 165 14 8 330 5 314 4.32e-50 172
MsG0680034923.01.T01 MTR_5g096900 36.826 334 151 12 10 326 5 295 1.13e-48 166
MsG0680034923.01.T01 MTR_1g076810 43.542 271 117 12 1 265 1 241 1.62e-48 173
MsG0680034923.01.T01 MTR_4g014370 34.195 348 175 12 13 336 7 324 1.66e-48 168
MsG0680034923.01.T01 MTR_1g053510 39.359 343 156 17 5 327 2 312 1.92e-48 168
MsG0680034923.01.T01 MTR_5g091140 36.648 352 162 13 2 343 33 333 2.66e-48 168
MsG0680034923.01.T01 MTR_8g046310 37.013 308 139 8 6 304 2 263 3.87e-48 166
MsG0680034923.01.T01 MTR_5g059430 36.490 359 151 17 14 336 23 340 4.74e-48 167
MsG0680034923.01.T01 MTR_5g094350 36.093 302 155 7 11 306 7 276 8.69e-48 162
MsG0680034923.01.T01 MTR_8g063370 34.356 326 165 12 17 337 24 305 1.67e-46 162
MsG0680034923.01.T01 MTR_2g066150 35.045 331 147 11 6 332 1 267 2.13e-46 161
MsG0680034923.01.T01 MTR_8g033140 36.310 336 164 13 13 326 15 322 3.31e-46 162
MsG0680034923.01.T01 MTR_8g013000 36.716 335 170 12 13 336 6 309 4.59e-46 162
MsG0680034923.01.T01 MTR_5g097820 38.062 289 128 12 5 277 7 260 6.42e-46 157
MsG0680034923.01.T01 MTR_8g018110 36.111 324 145 13 6 303 2 289 1.32e-45 160
MsG0680034923.01.T01 MTR_5g092860 37.086 302 150 9 35 332 20 285 3.39e-45 158
MsG0680034923.01.T01 MTR_5g072060 36.795 337 151 13 12 336 1 287 3.58e-45 163
MsG0680034923.01.T01 MTR_8g063330 34.012 344 178 13 6 336 1 308 5.05e-44 158
MsG0680034923.01.T01 MTR_5g096910 34.384 349 173 12 1 336 1 306 1.52e-43 155
MsG0680034923.01.T01 MTR_1g007430 36.364 308 148 12 28 328 7 273 5.93e-43 151
MsG0680034923.01.T01 MTR_2g064950 35.608 337 166 14 13 336 6 304 8.86e-42 150
MsG0680034923.01.T01 MTR_6g090260 66.981 106 34 1 231 336 73 177 1.65e-41 146
MsG0680034923.01.T01 MTR_6g090260 76.471 68 13 2 1 65 1 68 7.90e-23 96.3
MsG0680034923.01.T01 MTR_1g050308 40.079 252 108 11 5 250 22 236 3.04e-41 147
MsG0680034923.01.T01 MTR_8g046220 35.048 311 147 10 15 304 15 291 3.35e-40 146
MsG0680034923.01.T01 MTR_8g033090 35.262 363 175 17 7 342 3 332 6.54e-40 145
MsG0680034923.01.T01 MTR_8g033110 36.709 316 151 14 6 302 2 287 6.76e-40 145
MsG0680034923.01.T01 MTR_8g032980 33.616 354 170 12 7 326 4 326 2.01e-39 144
MsG0680034923.01.T01 MTR_8g046200 34.356 326 145 12 15 303 14 307 5.81e-39 143
MsG0680034923.01.T01 MTR_5g074800 35.517 290 148 12 56 336 1 260 9.12e-37 135
MsG0680034923.01.T01 MTR_5g076490 34.722 288 146 8 54 336 1 251 9.70e-37 135
MsG0680034923.01.T01 MTR_2g104260 35.644 303 154 14 7 297 4 277 3.07e-36 132
MsG0680034923.01.T01 MTR_1g115205 34.926 272 126 9 1 272 17 237 8.62e-36 130
MsG0680034923.01.T01 MTR_1g098180 33.648 318 134 13 14 326 16 261 8.88e-36 133
MsG0680034923.01.T01 MTR_7g111700 34.911 338 137 14 1 324 1 269 6.21e-34 128
MsG0680034923.01.T01 MTR_5g043605 30.189 318 132 5 13 330 21 248 1.16e-33 128
MsG0680034923.01.T01 MTR_5g064700 31.313 297 169 9 56 332 1 282 2.25e-33 127
MsG0680034923.01.T01 MTR_5g037190 35.870 276 125 10 1 271 1 229 3.95e-33 123
MsG0680034923.01.T01 MTR_8g064790 39.691 194 98 6 4 194 2 179 3.56e-32 123
MsG0680034923.01.T01 MTR_8g073570 41.799 189 88 7 8 193 1 170 7.86e-32 117
MsG0680034923.01.T01 MTR_5g022850 31.250 320 146 11 13 329 12 260 2.53e-31 121
MsG0680034923.01.T01 MTR_8g063310 30.294 340 176 18 13 337 18 311 4.21e-31 121
MsG0680034923.01.T01 MTR_8g063350 30.060 336 146 13 9 337 6 259 3.98e-30 117
MsG0680034923.01.T01 MTR_4g007940 30.450 289 141 11 67 332 2 253 1.82e-28 113
MsG0680034923.01.T01 MTR_5g068490 33.047 233 104 8 98 330 8 188 5.94e-27 107
MsG0680034923.01.T01 MTR_5g097650 43.030 165 69 8 1 156 1 149 1.26e-26 103
MsG0680034923.01.T01 MTR_4g094400 59.091 88 25 1 260 336 6 93 1.52e-26 102
MsG0680034923.01.T01 MTR_6g087660 64.634 82 28 1 1 81 1 82 2.35e-26 103
MsG0680034923.01.T01 MTR_8g036970 44.056 143 68 5 21 156 2 139 1.26e-24 103
MsG0680034923.01.T01 MTR_8g036970 47.423 97 37 4 233 328 147 230 5.80e-16 78.6
MsG0680034923.01.T01 MTR_1g050312 31.890 254 98 11 89 330 16 206 3.86e-24 100
MsG0680034923.01.T01 MTR_6g004450 38.323 167 77 6 175 334 42 189 7.63e-24 99.4
MsG0680034923.01.T01 MTR_3g019290 35.751 193 68 5 14 202 11 151 1.29e-23 96.3
MsG0680034923.01.T01 MTR_5g096070 42.763 152 50 6 13 159 22 141 1.35e-23 95.9
MsG0680034923.01.T01 MTR_3g018750 38.415 164 81 5 181 336 2 153 1.77e-23 97.4
MsG0680034923.01.T01 MTR_6g009350 38.788 165 69 7 182 334 8 152 2.70e-22 93.2
MsG0680034923.01.T01 MTR_5g091150 32.314 229 89 6 117 345 40 202 4.35e-22 92.8
MsG0680034923.01.T01 MTR_4g016000 34.343 198 81 7 144 336 2 155 3.95e-21 90.5
MsG0680034923.01.T01 MTR_4g014370 40.000 140 78 4 3 138 9 146 5.03e-21 92.8
MsG0680034923.01.T01 MTR_4g014370 35.185 108 57 3 233 336 154 252 3.51e-11 63.9
MsG0680034923.01.T01 MTR_5g086590 34.392 189 86 5 139 326 13 164 6.03e-21 90.5
MsG0680034923.01.T01 MTR_8g041440 61.644 73 26 1 8 80 6 76 9.87e-21 86.7
MsG0680034923.01.T01 MTR_3g047580 36.667 150 70 5 6 153 1 127 2.57e-19 83.2
MsG0680034923.01.T01 MTR_6g079730 60.606 66 25 1 271 336 1 65 5.08e-19 82.8
MsG0680034923.01.T01 MTR_1g047080 31.868 273 129 14 54 322 1 220 4.22e-18 83.6
MsG0680034923.01.T01 MTR_2g021920 36.943 157 80 8 182 329 37 183 2.59e-17 81.3
MsG0680034923.01.T01 MTR_3g013740 26.074 326 186 13 13 328 40 320 4.81e-16 79.0
MsG0680034923.01.T01 MTR_2g031640 25.000 320 182 10 20 334 9 275 7.59e-16 78.2
MsG0680034923.01.T01 MTR_5g086780 69.492 59 17 1 1 59 1 58 1.36e-15 75.5
MsG0680034923.01.T01 MTR_2g024110 25.316 316 178 13 17 330 4 263 2.02e-15 77.0
MsG0680034923.01.T01 MTR_7g017150 46.939 98 44 4 233 328 82 173 2.63e-15 75.5
MsG0680034923.01.T01 MTR_2g426070 26.087 322 184 14 25 334 14 293 2.79e-15 76.6
MsG0680034923.01.T01 MTR_7g017120 44.792 96 49 3 233 328 81 172 1.26e-14 73.6
MsG0680034923.01.T01 MTR_5g098950 31.383 188 75 7 141 328 90 223 2.71e-14 72.4
MsG0680034923.01.T01 MTR_2g028130 24.629 337 190 12 13 331 1 291 2.86e-14 73.6
MsG0680034923.01.T01 MTR_4g133170 23.779 307 181 9 20 326 7 260 3.61e-14 73.2
MsG0680034923.01.T01 MTR_4g117770 27.437 277 139 12 1 274 1 218 6.53e-14 72.4
MsG0680034923.01.T01 MTR_4g117810 27.437 277 139 12 1 274 1 218 6.53e-14 72.4
MsG0680034923.01.T01 MTR_2g426090 25.237 317 175 14 25 327 14 282 6.66e-14 72.8
MsG0680034923.01.T01 MTR_5g023970 43.158 95 50 2 231 325 9 99 1.11e-13 69.3
MsG0680034923.01.T01 MTR_8g013010 26.059 307 168 13 9 307 43 298 1.66e-13 70.9
MsG0680034923.01.T01 MTR_2g028110 24.920 313 178 12 20 326 7 268 1.72e-13 71.2
MsG0680034923.01.T01 MTR_2g028160 24.036 337 194 13 13 331 1 293 3.52e-13 70.5
MsG0680034923.01.T01 MTR_4g070550 24.779 339 187 15 13 331 1 291 4.19e-13 70.1
MsG0680034923.01.T01 MTR_2g031720 23.607 305 174 11 17 319 6 253 4.82e-13 70.1
MsG0680034923.01.T01 MTR_2g028140 25.356 351 176 17 13 331 1 297 6.01e-13 69.7
MsG0680034923.01.T01 MTR_7g019250 34.444 180 84 8 161 336 202 351 7.14e-13 69.7
MsG0680034923.01.T01 MTR_5g057790 24.382 283 153 10 20 295 49 277 9.16e-13 68.9
MsG0680034923.01.T01 MTR_2g026010 25.329 304 156 15 36 326 23 268 1.06e-12 68.9
MsG0680034923.01.T01 MTR_3g013770 27.070 314 176 15 20 328 36 301 2.02e-12 68.2
MsG0680034923.01.T01 MTR_1g068860 26.367 311 172 12 20 326 7 264 2.11e-12 67.8
MsG0680034923.01.T01 MTR_4g061340 26.882 279 164 12 2 277 10 251 7.28e-12 66.2
MsG0680034923.01.T01 MTR_5g057710 22.684 313 179 10 20 325 49 305 1.73e-11 65.1
MsG0680034923.01.T01 MTR_1g023400 26.255 259 130 10 20 271 6 210 1.84e-11 65.1
MsG0680034923.01.T01 MTR_1g094210 24.706 255 134 9 17 270 57 254 1.97e-11 65.1
MsG0680034923.01.T01 MTR_1g094210 24.706 255 134 9 17 270 57 254 2.18e-11 65.1
MsG0680034923.01.T01 MTR_7g070440 25.843 267 136 11 14 271 4 217 2.64e-11 64.7
MsG0680034923.01.T01 MTR_3g047540 43.820 89 38 4 233 320 6 83 2.65e-11 59.7
MsG0680034923.01.T01 MTR_7g101200 26.263 198 92 7 138 329 37 186 4.07e-11 62.8
MsG0680034923.01.T01 MTR_1g097560 24.172 302 165 14 8 306 27 267 4.20e-11 63.9
MsG0680034923.01.T01 MTR_3g083010 22.946 353 202 15 12 342 1 305 5.25e-11 63.9
MsG0680034923.01.T01 MTR_8g099460 24.921 317 178 13 20 327 8 273 7.33e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 73 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCATCTTCATCATATTTA+TGG 0.110150 6:-96672626 None:intergenic
TGGCCGCCGGGGAAGAATTT+TGG 0.131280 6:+96672906 MsG0680034923.01.T01:CDS
TGGTTATGACCTTGTTAATA+AGG 0.217272 6:+96672791 MsG0680034923.01.T01:CDS
AGCTTGATTGTTTGGGTAGC+TGG 0.256226 6:-96672672 None:intergenic
ATAAACTTGGCGGTGTTAAA+TGG 0.277192 6:+96673182 MsG0680034923.01.T01:CDS
GAGGAAGGAGCTTGATTGTT+TGG 0.314387 6:-96672680 None:intergenic
TCATCTTCGTCCGAGGAAAT+AGG 0.322393 6:-96673134 None:intergenic
AACATCAGCTTCATAACGTT+TGG 0.323354 6:-96672481 None:intergenic
TTTGGGTGAGCTCGGTATAA+AGG 0.352238 6:+96673258 MsG0680034923.01.T01:CDS
ATGTCCCGTCTTCATGGATT+TGG 0.360899 6:+96672771 MsG0680034923.01.T01:CDS
AGGAAGGAGCTTGATTGTTT+GGG 0.379764 6:-96672679 None:intergenic
TCAAATTTCGACTGGCCGCC+GGG 0.380938 6:+96672894 MsG0680034923.01.T01:CDS
TTGAATGCGTTCGCAAATCA+TGG 0.384751 6:+96672349 MsG0680034923.01.T01:CDS
CATAACGTTTGGGCTCTGTT+AGG 0.390686 6:-96672470 None:intergenic
AAGAGGCAACTTTGAGAGAA+TGG 0.390893 6:-96672310 None:intergenic
CTGGGTTCCACAATACAATT+TGG 0.396588 6:-96672653 None:intergenic
AAACGCTTAAAAGATTTAAG+AGG 0.406317 6:-96672327 None:intergenic
GATGTTATGTCCCGTCTTCA+TGG 0.413315 6:+96672765 MsG0680034923.01.T01:CDS
TCAGTACGACAAGAAGAATA+AGG 0.425598 6:-96672990 None:intergenic
AGAAGATCCTTATCTTCATC+GGG 0.429359 6:-96672735 None:intergenic
TAGTCCCGCTGCACCATGTT+TGG 0.430134 6:+96673073 MsG0680034923.01.T01:CDS
CCTTGTAACACCTATTTCCT+CGG 0.438410 6:+96673124 MsG0680034923.01.T01:CDS
GCTTGATTGTTTGGGTAGCT+GGG 0.440375 6:-96672671 None:intergenic
ACATCAGCTTCATAACGTTT+GGG 0.441417 6:-96672480 None:intergenic
TAAACTTGGCGGTGTTAAAT+GGG 0.446226 6:+96673183 MsG0680034923.01.T01:CDS
TTCAAATTTCGACTGGCCGC+CGG 0.449716 6:+96672893 MsG0680034923.01.T01:CDS
CAAGGTCATTCGATATATAG+AGG 0.451531 6:+96672818 MsG0680034923.01.T01:CDS
TCCTCCCAGTGATATTGACT+TGG 0.468274 6:+96672698 MsG0680034923.01.T01:CDS
CTCTCCACGAGTTACTTCTT+AGG 0.479597 6:-96672950 None:intergenic
CTCAGCCAAGTCAATATCAC+TGG 0.481305 6:-96672703 None:intergenic
TCCATCGTAGTTAGAATGAT+GGG 0.483333 6:-96672436 None:intergenic
AAGAAGATCCTTATCTTCAT+CGG 0.497683 6:-96672736 None:intergenic
GTCTATCCCCGATGAAGATA+AGG 0.501261 6:+96672728 MsG0680034923.01.T01:CDS
ATCCATCGTAGTTAGAATGA+TGG 0.507225 6:-96672437 None:intergenic
TATCTTTCTTACACAACCAA+TGG 0.509544 6:-96673043 None:intergenic
ACGCTATATCATCAGATACA+TGG 0.514047 6:-96672284 None:intergenic
TCTATTCTCTTGCTGGTGAG+AGG 0.516733 6:+96672505 MsG0680034923.01.T01:CDS
GATACCAAACATGGTGCAGC+GGG 0.519150 6:-96673077 None:intergenic
ACACTCCATCCATGTAGACT+TGG 0.520419 6:-96673019 None:intergenic
ACATGGATGGAGTGTGCCAT+TGG 0.525649 6:+96673027 MsG0680034923.01.T01:CDS
ACAGTACTTCAAATTTCGAC+TGG 0.535871 6:+96672886 MsG0680034923.01.T01:CDS
TTATTCTTCTTGTCGTACTG+AGG 0.541293 6:+96672992 MsG0680034923.01.T01:CDS
TGAGGGCACCCAAGTCTACA+TGG 0.542607 6:+96673010 MsG0680034923.01.T01:CDS
AGGCTATACATCTCCCATGA+TGG 0.546940 6:-96672930 None:intergenic
CACTCCATCCATGTAGACTT+GGG 0.548105 6:-96673018 None:intergenic
CCGAGGAAATAGGTGTTACA+AGG 0.549954 6:-96673124 None:intergenic
TATTCTTCTTGTCGTACTGA+GGG 0.550887 6:+96672993 MsG0680034923.01.T01:CDS
TGGAGCAAAGTGGATAAACT+TGG 0.554105 6:+96673169 MsG0680034923.01.T01:CDS
CGCTATATCATCAGATACAT+GGG 0.560703 6:-96672283 None:intergenic
AGTCAATATCACTGGGAGGA+AGG 0.561097 6:-96672695 None:intergenic
CCTTGTAGTCCTTATTAACA+AGG 0.564703 6:-96672800 None:intergenic
GATGGCCCAAAATTCTTCCC+CGG 0.565259 6:-96672912 None:intergenic
CCTTGTTAATAAGGACTACA+AGG 0.572397 6:+96672800 MsG0680034923.01.T01:CDS
GGCACCCAAGTCTACATGGA+TGG 0.576024 6:+96673014 MsG0680034923.01.T01:CDS
AGTGGCTGTAACGAATGAAA+TGG 0.585953 6:+96672254 MsG0680034923.01.T01:CDS
TAAGAAATTGCTGCAAAACA+TGG 0.587313 6:-96672403 None:intergenic
CCCCATCATTCTAACTACGA+TGG 0.594086 6:+96672435 MsG0680034923.01.T01:CDS
ATAACCAAATCCATGAAGAC+GGG 0.603933 6:-96672775 None:intergenic
CATAACCAAATCCATGAAGA+CGG 0.604936 6:-96672776 None:intergenic
GTGAAACGAGGTCAAGGCAA+CGG 0.607298 6:-96673207 None:intergenic
ATCATGACCAAATTGTATTG+TGG 0.608800 6:+96672646 MsG0680034923.01.T01:CDS
AAAACAATCATCTTCGTCCG+AGG 0.627091 6:-96673141 None:intergenic
AGCAAAGTGGATAAACTTGG+CGG 0.627094 6:+96673172 MsG0680034923.01.T01:CDS
CAAATTTCGACTGGCCGCCG+GGG 0.643344 6:+96672895 MsG0680034923.01.T01:CDS
TGATACCAAACATGGTGCAG+CGG 0.650810 6:-96673078 None:intergenic
CCATCGTAGTTAGAATGATG+GGG 0.653236 6:-96672435 None:intergenic
TCAGCCAAGTCAATATCACT+GGG 0.659160 6:-96672702 None:intergenic
AAGTAAAATGATACCAAACA+TGG 0.660782 6:-96673086 None:intergenic
ATAGCCTAAGAAGTAACTCG+TGG 0.663042 6:+96672946 MsG0680034923.01.T01:CDS
GGCCCAAAATTCTTCCCCGG+CGG 0.665547 6:-96672909 None:intergenic
GCCAAGTCAATATCACTGGG+AGG 0.711122 6:-96672699 None:intergenic
GAAGATCCTTATCTTCATCG+GGG 0.733888 6:-96672734 None:intergenic
TTGGATGGAGAAGACAACAG+TGG 0.747342 6:+96672236 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTATGATATTCGTATTTT+TGG + Chr6:96672573-96672592 MsG0680034923.01.T01:CDS 15.0%
!!! ACTTTTCACATATCAATTTT+GGG + Chr6:96673241-96673260 MsG0680034923.01.T01:CDS 20.0%
!!! TACTTTTCACATATCAATTT+TGG + Chr6:96673240-96673259 MsG0680034923.01.T01:CDS 20.0%
! AAACGCTTAAAAGATTTAAG+AGG - Chr6:96672330-96672349 None:intergenic 25.0%
! AAGTAAAATGATACCAAACA+TGG - Chr6:96673089-96673108 None:intergenic 25.0%
! GATCATCTTCATCATATTTA+TGG - Chr6:96672629-96672648 None:intergenic 25.0%
!!! GAAGATGATTGTTTTAATGT+TGG + Chr6:96673149-96673168 MsG0680034923.01.T01:CDS 25.0%
!!! GATATTCGTATTTTTGGTTT+TGG + Chr6:96672579-96672598 MsG0680034923.01.T01:CDS 25.0%
AAGAAGATCCTTATCTTCAT+CGG - Chr6:96672739-96672758 None:intergenic 30.0%
ATCATGACCAAATTGTATTG+TGG + Chr6:96672646-96672665 MsG0680034923.01.T01:CDS 30.0%
TAAGAAATTGCTGCAAAACA+TGG - Chr6:96672406-96672425 None:intergenic 30.0%
TATCTTTCTTACACAACCAA+TGG - Chr6:96673046-96673065 None:intergenic 30.0%
TGGTTATGACCTTGTTAATA+AGG + Chr6:96672791-96672810 MsG0680034923.01.T01:CDS 30.0%
! ATCATTTTACTTGAGCAATG+AGG + Chr6:96673097-96673116 MsG0680034923.01.T01:CDS 30.0%
! TGAAGTGATGATTTTCAAAC+AGG - Chr6:96672380-96672399 None:intergenic 30.0%
!!! TTTGGTTTTGGTAGTATTAG+TGG + Chr6:96672591-96672610 MsG0680034923.01.T01:CDS 30.0%
AACATCAGCTTCATAACGTT+TGG - Chr6:96672484-96672503 None:intergenic 35.0%
ACAGTACTTCAAATTTCGAC+TGG + Chr6:96672886-96672905 MsG0680034923.01.T01:CDS 35.0%
ACATCAGCTTCATAACGTTT+GGG - Chr6:96672483-96672502 None:intergenic 35.0%
ACGCTATATCATCAGATACA+TGG - Chr6:96672287-96672306 None:intergenic 35.0%
AGAAGATCCTTATCTTCATC+GGG - Chr6:96672738-96672757 None:intergenic 35.0%
ATAACCAAATCCATGAAGAC+GGG - Chr6:96672778-96672797 None:intergenic 35.0%
ATCCATCGTAGTTAGAATGA+TGG - Chr6:96672440-96672459 None:intergenic 35.0%
CAAGGTCATTCGATATATAG+AGG + Chr6:96672818-96672837 MsG0680034923.01.T01:CDS 35.0%
CATAACCAAATCCATGAAGA+CGG - Chr6:96672779-96672798 None:intergenic 35.0%
CCTTGTAGTCCTTATTAACA+AGG - Chr6:96672803-96672822 None:intergenic 35.0%
CCTTGTTAATAAGGACTACA+AGG + Chr6:96672800-96672819 MsG0680034923.01.T01:CDS 35.0%
CGCTATATCATCAGATACAT+GGG - Chr6:96672286-96672305 None:intergenic 35.0%
TATTCTTCTTGTCGTACTGA+GGG + Chr6:96672993-96673012 MsG0680034923.01.T01:CDS 35.0%
TCAGTACGACAAGAAGAATA+AGG - Chr6:96672993-96673012 None:intergenic 35.0%
TCCATCGTAGTTAGAATGAT+GGG - Chr6:96672439-96672458 None:intergenic 35.0%
TTATTCTTCTTGTCGTACTG+AGG + Chr6:96672992-96673011 MsG0680034923.01.T01:CDS 35.0%
! ATAAACTTGGCGGTGTTAAA+TGG + Chr6:96673182-96673201 MsG0680034923.01.T01:CDS 35.0%
! GATGTTTTCTATTCTCTTGC+TGG + Chr6:96672498-96672517 MsG0680034923.01.T01:CDS 35.0%
! TAAACTTGGCGGTGTTAAAT+GGG + Chr6:96673183-96673202 MsG0680034923.01.T01:CDS 35.0%
!! ATATCAATTTTGGGTGAGCT+CGG + Chr6:96673250-96673269 MsG0680034923.01.T01:CDS 35.0%
!! GTTTTAATGTTGGAGCAAAG+TGG + Chr6:96673159-96673178 MsG0680034923.01.T01:CDS 35.0%
AAAACAATCATCTTCGTCCG+AGG - Chr6:96673144-96673163 None:intergenic 40.0%
AGCAAAGTGGATAAACTTGG+CGG + Chr6:96673172-96673191 MsG0680034923.01.T01:CDS 40.0%
AGGAAGGAGCTTGATTGTTT+GGG - Chr6:96672682-96672701 None:intergenic 40.0%
AGTGGCTGTAACGAATGAAA+TGG + Chr6:96672254-96672273 MsG0680034923.01.T01:CDS 40.0%
ATAGCCTAAGAAGTAACTCG+TGG + Chr6:96672946-96672965 MsG0680034923.01.T01:CDS 40.0%
CCATCGTAGTTAGAATGATG+GGG - Chr6:96672438-96672457 None:intergenic 40.0%
CCTTGTAACACCTATTTCCT+CGG + Chr6:96673124-96673143 MsG0680034923.01.T01:CDS 40.0%
CTGGGTTCCACAATACAATT+TGG - Chr6:96672656-96672675 None:intergenic 40.0%
GAAGATCCTTATCTTCATCG+GGG - Chr6:96672737-96672756 None:intergenic 40.0%
TCAGCCAAGTCAATATCACT+GGG - Chr6:96672705-96672724 None:intergenic 40.0%
TGGAGCAAAGTGGATAAACT+TGG + Chr6:96673169-96673188 MsG0680034923.01.T01:CDS 40.0%
TTGAATGCGTTCGCAAATCA+TGG + Chr6:96672349-96672368 MsG0680034923.01.T01:CDS 40.0%
! AAGAGGCAACTTTGAGAGAA+TGG - Chr6:96672313-96672332 None:intergenic 40.0%
! GAAGAATTTTGGGCCATCAT+GGG + Chr6:96672917-96672936 MsG0680034923.01.T01:CDS 40.0%
! TAGTCGTCTTTTGTGAAACG+AGG - Chr6:96673222-96673241 None:intergenic 40.0%
! TCTTTTGTGAAACGAGGTCA+AGG - Chr6:96673216-96673235 None:intergenic 40.0%
ACACTCCATCCATGTAGACT+TGG - Chr6:96673022-96673041 None:intergenic 45.0%
AGCTTGATTGTTTGGGTAGC+TGG - Chr6:96672675-96672694 None:intergenic 45.0%
AGGCTATACATCTCCCATGA+TGG - Chr6:96672933-96672952 None:intergenic 45.0%
AGTCAATATCACTGGGAGGA+AGG - Chr6:96672698-96672717 None:intergenic 45.0%
ATGTCCCGTCTTCATGGATT+TGG + Chr6:96672771-96672790 MsG0680034923.01.T01:CDS 45.0%
CACTCCATCCATGTAGACTT+GGG - Chr6:96673021-96673040 None:intergenic 45.0%
CATAACGTTTGGGCTCTGTT+AGG - Chr6:96672473-96672492 None:intergenic 45.0%
CCCCATCATTCTAACTACGA+TGG + Chr6:96672435-96672454 MsG0680034923.01.T01:CDS 45.0%
CTCAGCCAAGTCAATATCAC+TGG - Chr6:96672706-96672725 None:intergenic 45.0%
CTCTCCACGAGTTACTTCTT+AGG - Chr6:96672953-96672972 None:intergenic 45.0%
GAGGAAGGAGCTTGATTGTT+TGG - Chr6:96672683-96672702 None:intergenic 45.0%
GATGTTATGTCCCGTCTTCA+TGG + Chr6:96672765-96672784 MsG0680034923.01.T01:CDS 45.0%
GCTTGATTGTTTGGGTAGCT+GGG - Chr6:96672674-96672693 None:intergenic 45.0%
GTCTATCCCCGATGAAGATA+AGG + Chr6:96672728-96672747 MsG0680034923.01.T01:CDS 45.0%
TCATCTTCGTCCGAGGAAAT+AGG - Chr6:96673137-96673156 None:intergenic 45.0%
TCTATTCTCTTGCTGGTGAG+AGG + Chr6:96672505-96672524 MsG0680034923.01.T01:CDS 45.0%
TGATACCAAACATGGTGCAG+CGG - Chr6:96673081-96673100 None:intergenic 45.0%
! CCGAGGAAATAGGTGTTACA+AGG - Chr6:96673127-96673146 None:intergenic 45.0%
! GGAAGAATTTTGGGCCATCA+TGG + Chr6:96672916-96672935 MsG0680034923.01.T01:CDS 45.0%
! TCCTCCCAGTGATATTGACT+TGG + Chr6:96672698-96672717 MsG0680034923.01.T01:CDS 45.0%
ACATGGATGGAGTGTGCCAT+TGG + Chr6:96673027-96673046 MsG0680034923.01.T01:CDS 50.0%
GATACCAAACATGGTGCAGC+GGG - Chr6:96673080-96673099 None:intergenic 50.0%
GATGGCCCAAAATTCTTCCC+CGG - Chr6:96672915-96672934 None:intergenic 50.0%
GCCAAGTCAATATCACTGGG+AGG - Chr6:96672702-96672721 None:intergenic 50.0%
GTGAAACGAGGTCAAGGCAA+CGG - Chr6:96673210-96673229 None:intergenic 50.0%
! TTCAAATTTCGACTGGCCGC+CGG + Chr6:96672893-96672912 MsG0680034923.01.T01:CDS 50.0%
GGCACCCAAGTCTACATGGA+TGG + Chr6:96673014-96673033 MsG0680034923.01.T01:CDS 55.0%
TAGTCCCGCTGCACCATGTT+TGG + Chr6:96673073-96673092 MsG0680034923.01.T01:CDS 55.0%
! TCAAATTTCGACTGGCCGCC+GGG + Chr6:96672894-96672913 MsG0680034923.01.T01:CDS 55.0%
!! TGAGGGCACCCAAGTCTACA+TGG + Chr6:96673010-96673029 MsG0680034923.01.T01:CDS 55.0%
GGCCCAAAATTCTTCCCCGG+CGG - Chr6:96672912-96672931 None:intergenic 60.0%
! CAAATTTCGACTGGCCGCCG+GGG + Chr6:96672895-96672914 MsG0680034923.01.T01:CDS 60.0%
! GGCCGCCGGGGAAGAATTTT+GGG + Chr6:96672907-96672926 MsG0680034923.01.T01:CDS 60.0%
! TGGCCGCCGGGGAAGAATTT+TGG + Chr6:96672906-96672925 MsG0680034923.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr6 gene 96672240 96673277 96672240 ID=MsG0680034923.01;Name=MsG0680034923.01
Chr6 mRNA 96672240 96673277 96672240 ID=MsG0680034923.01.T01;Parent=MsG0680034923.01;Name=MsG0680034923.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|345
Chr6 exon 96672240 96673277 96672240 ID=MsG0680034923.01.T01:exon:6968;Parent=MsG0680034923.01.T01
Chr6 CDS 96672240 96673277 96672240 ID=MsG0680034923.01.T01:cds;Parent=MsG0680034923.01.T01
Gene Sequence

>MsG0680034923.01.T01

ATGGAGAAGACAACAGTGGCTGTAACGAATGAAATGGTGAGTACCCATGTATCTGATGATATAGCGTTTTCCATTCTCTCAAAGTTGCCTCTTAAATCTTTTAAGCGTTTTGAATGCGTTCGCAAATCATGGTCTCTCCTGTTTGAAAATCATCACTTCATGACCATGTTTTGCAGCAATTTCTTATCTAATTCTCCCCATCATTCTAACTACGATGGATCATCTCTCATCCTAACAGAGCCCAAACGTTATGAAGCTGATGTTTTCTATTCTCTTGCTGGTGAGAGGTTTAAGAAAAAAATCAAATTAGATTTCTCAAATCGATTTGAAGAAAATTATGATATTCGTATTTTTGGTTTTGGTAGTATTAGTGGCATACTTTGTCTCCATAAATATGATGAAGATGATCATGACCAAATTGTATTGTGGAACCCAGCTACCCAAACAATCAAGCTCCTTCCTCCCAGTGATATTGACTTGGCTGAGTCGTCTATCCCCGATGAAGATAAGGATCTTCTTGATATTGATGTTATGTCCCGTCTTCATGGATTTGGTTATGACCTTGTTAATAAGGACTACAAGGTCATTCGATATATAGAGGTTTCTATAGAGATAAGTGATTACTCTAGTGACGACGAAAAACTTGACAGTACTTCAAATTTCGACTGGCCGCCGGGGAAGAATTTTGGGCCATCATGGGAGATGTATAGCCTAAGAAGTAACTCGTGGAGAGAGCTTAATGTTGACATGCCTTATTCTTCTTGTCGTACTGAGGGCACCCAAGTCTACATGGATGGAGTGTGCCATTGGTTGTGTAAGAAAGATAAAAAATATAGTCCCGCTGCACCATGTTTGGTATCATTTTACTTGAGCAATGAGGTGTTCCTTGTAACACCTATTTCCTCGGACGAAGATGATTGTTTTAATGTTGGAGCAAAGTGGATAAACTTGGCGGTGTTAAATGGGTCCGTTGCCTTGACCTCGTTTCACAAAAGACGACTACTTTTCACATATCAATTTTGGGTGAGCTCGGTATAA
Protein sequence

>MsG0680034923.01.T01

MEKTTVAVTNEMVSTHVSDDIAFSILSKLPLKSFKRFECVRKSWSLLFENHHFMTMFCSNFLSNSPHHSNYDGSSLILTEPKRYEADVFYSLAGERFKKKIKLDFSNRFEENYDIRIFGFGSISGILCLHKYDEDDHDQIVLWNPATQTIKLLPPSDIDLAESSIPDEDKDLLDIDVMSRLHGFGYDLVNKDYKVIRYIEVSIEISDYSSDDEKLDSTSNFDWPPGKNFGPSWEMYSLRSNSWRELNVDMPYSSCRTEGTQVYMDGVCHWLCKKDKKYSPAAPCLVSFYLSNEVFLVTPISSDEDDCFNVGAKWINLAVLNGSVALTSFHKRRLLFTYQFWVSSV*