AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001901.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001901.01.T01 MTR_4g007940 81.728 301 51 3 9 307 1 299 3.35e-180 501
MsG0180001901.01.T01 MTR_8g063330 63.430 309 96 6 1 305 53 348 1.05e-122 360
MsG0180001901.01.T01 MTR_0831s0010 58.117 308 110 3 1 307 89 378 1.66e-119 350
MsG0180001901.01.T01 MTR_4g016000 72.770 213 34 2 88 300 2 190 5.93e-106 308
MsG0180001901.01.T01 MTR_0129s0070 43.354 316 154 5 1 308 56 354 1.36e-79 247
MsG0180001901.01.T01 MTR_0129s0120 43.038 316 155 5 1 308 56 354 1.05e-77 242
MsG0180001901.01.T01 MTR_8g063440 43.590 312 151 8 1 308 51 341 8.34e-75 234
MsG0180001901.01.T01 MTR_7g019250 57.075 212 66 3 97 307 206 393 1.63e-70 226
MsG0180001901.01.T01 MTR_4g055820 42.991 321 152 9 2 306 54 359 4.20e-69 219
MsG0180001901.01.T01 MTR_8g464770 40.836 311 156 9 2 308 52 338 8.59e-69 219
MsG0180001901.01.T01 MTR_2g104340 42.508 327 147 12 2 308 50 355 1.73e-67 216
MsG0180001901.01.T01 MTR_2g066170 42.188 320 145 12 1 307 51 343 1.92e-66 213
MsG0180001901.01.T01 MTR_2g021800 38.348 339 161 9 1 308 55 376 1.35e-65 212
MsG0180001901.01.T01 MTR_3g011120 43.949 314 140 10 2 308 54 338 1.86e-65 210
MsG0180001901.01.T01 MTR_5g097330 39.450 327 158 12 1 308 55 360 7.75e-65 209
MsG0180001901.01.T01 MTR_2g066200 41.049 324 145 12 1 307 51 345 1.08e-62 203
MsG0180001901.01.T01 MTR_2g022000 37.870 338 163 11 1 308 55 375 2.26e-62 204
MsG0180001901.01.T01 MTR_2g021770 38.253 332 162 10 1 307 51 364 2.45e-62 203
MsG0180001901.01.T01 MTR_8g012090 37.771 323 132 7 1 308 55 323 5.50e-62 200
MsG0180001901.01.T01 MTR_8g041270 39.701 335 156 12 1 308 50 365 3.29e-60 197
MsG0180001901.01.T01 MTR_6g086825 38.110 328 163 11 1 307 109 417 3.44e-60 199
MsG0180001901.01.T01 MTR_7g017040 40.798 326 151 12 1 308 55 356 1.63e-58 193
MsG0180001901.01.T01 MTR_6g080680 38.369 331 146 12 1 308 58 353 2.14e-58 192
MsG0180001901.01.T01 MTR_5g074800 40.190 316 163 10 1 305 1 301 3.59e-58 190
MsG0180001901.01.T01 MTR_5g097350 38.871 319 155 11 1 307 55 345 1.92e-57 190
MsG0180001901.01.T01 MTR_8g064890 40.909 286 129 10 34 305 84 343 2.72e-57 189
MsG0180001901.01.T01 MTR_4g130330 36.778 329 160 10 3 308 45 348 3.41e-57 189
MsG0180001901.01.T01 MTR_5g097220 37.798 336 156 8 6 308 49 364 5.03e-57 189
MsG0180001901.01.T01 MTR_8g467670 38.170 317 155 11 1 308 53 337 7.25e-57 188
MsG0180001901.01.T01 MTR_5g037680 39.077 325 150 13 1 308 47 340 1.10e-56 189
MsG0180001901.01.T01 MTR_7g079640 38.978 313 153 10 1 307 52 332 1.39e-56 187
MsG0180001901.01.T01 MTR_8g039500 40.312 320 142 13 1 307 51 334 3.11e-56 185
MsG0180001901.01.T01 MTR_2g065860 38.889 324 152 11 1 307 51 345 3.17e-56 187
MsG0180001901.01.T01 MTR_1g050312 41.727 278 111 11 36 305 16 250 3.89e-56 183
MsG0180001901.01.T01 MTR_0129s0050 38.253 332 157 11 1 305 53 363 8.91e-56 186
MsG0180001901.01.T01 MTR_4g117550 36.190 315 155 6 1 308 56 331 9.53e-56 185
MsG0180001901.01.T01 MTR_0054s0100 38.851 296 140 8 36 308 89 366 9.70e-56 186
MsG0180001901.01.T01 MTR_6g090180 37.764 331 163 13 1 308 86 396 2.09e-55 186
MsG0180001901.01.T01 MTR_7g069550 39.564 321 161 11 1 305 53 356 2.69e-55 184
MsG0180001901.01.T01 MTR_4g102410 36.728 324 173 9 1 308 100 407 2.73e-55 186
MsG0180001901.01.T01 MTR_0054s0090 39.322 295 138 8 36 308 89 364 3.57e-55 184
MsG0180001901.01.T01 MTR_5g064700 36.919 344 165 11 1 308 1 328 4.69e-55 183
MsG0180001901.01.T01 MTR_2g064950 37.003 327 160 10 1 308 49 348 6.46e-55 183
MsG0180001901.01.T01 MTR_8g013000 39.688 320 166 10 1 308 49 353 1.48e-54 183
MsG0180001901.01.T01 MTR_5g024260 39.210 329 153 12 1 308 61 363 7.00e-54 181
MsG0180001901.01.T01 MTR_2g104280 38.253 332 163 13 2 308 50 364 1.10e-53 181
MsG0180001901.01.T01 MTR_1g007450 38.006 321 169 10 1 308 51 354 1.75e-53 180
MsG0180001901.01.T01 MTR_6g079810 37.584 298 146 9 36 308 139 421 3.83e-53 181
MsG0180001901.01.T01 MTR_2g021950 37.413 286 151 8 34 307 87 356 6.46e-53 178
MsG0180001901.01.T01 MTR_5g069390 37.500 328 168 10 1 308 65 375 8.38e-53 180
MsG0180001901.01.T01 MTR_2g038680 35.522 335 166 11 1 307 55 367 1.07e-52 178
MsG0180001901.01.T01 MTR_2g066150 37.931 319 133 9 1 307 51 316 1.30e-52 176
MsG0180001901.01.T01 MTR_6g090250 38.291 316 137 12 36 308 82 382 1.67e-52 178
MsG0180001901.01.T01 MTR_4g028510 37.884 293 140 10 37 308 89 360 2.67e-52 178
MsG0180001901.01.T01 MTR_7g017130 37.742 310 164 10 1 300 57 347 2.98e-52 176
MsG0180001901.01.T01 MTR_7g017170 37.742 310 164 10 1 300 57 347 3.13e-52 177
MsG0180001901.01.T01 MTR_5g059430 36.127 346 156 13 1 308 65 383 9.08e-52 176
MsG0180001901.01.T01 MTR_2g034700 38.170 317 150 10 1 308 53 332 4.92e-51 172
MsG0180001901.01.T01 MTR_2g065840 37.231 325 155 12 1 307 51 344 5.97e-51 173
MsG0180001901.01.T01 MTR_5g096630 35.030 334 172 11 1 308 57 371 4.09e-50 171
MsG0180001901.01.T01 MTR_5g097680 36.859 312 144 8 36 308 90 387 4.81e-50 171
MsG0180001901.01.T01 MTR_1g007430 36.957 322 157 10 1 308 35 324 7.56e-50 168
MsG0180001901.01.T01 MTR_6g078010 36.170 329 151 10 1 308 3 293 7.91e-50 169
MsG0180001901.01.T01 MTR_6g079790 38.235 272 132 10 58 308 11 267 1.11e-49 167
MsG0180001901.01.T01 MTR_0134s0060 34.911 338 170 13 2 308 52 370 1.93e-49 169
MsG0180001901.01.T01 MTR_3g019290 52.830 159 39 1 1 159 53 175 1.40e-48 160
MsG0180001901.01.T01 MTR_4g014370 33.524 349 160 10 5 308 46 367 1.64e-48 167
MsG0180001901.01.T01 MTR_4g028660 34.237 295 155 10 33 308 83 357 3.01e-48 166
MsG0180001901.01.T01 MTR_4g027450 34.237 295 155 10 33 308 83 357 3.01e-48 166
MsG0180001901.01.T01 MTR_8g033140 35.735 347 155 14 1 308 58 375 7.82e-48 166
MsG0180001901.01.T01 MTR_6g478230 34.954 329 164 10 1 308 66 365 1.03e-47 165
MsG0180001901.01.T01 MTR_8g036970 36.137 321 116 11 1 308 37 281 4.45e-46 160
MsG0180001901.01.T01 MTR_4g028750 34.797 296 151 11 33 308 83 356 9.04e-46 159
MsG0180001901.01.T01 MTR_1g007410 35.294 323 173 12 1 308 61 362 1.85e-45 159
MsG0180001901.01.T01 MTR_6g461660 35.913 323 164 14 1 305 58 355 4.29e-45 158
MsG0180001901.01.T01 MTR_6g079770 33.432 338 177 10 1 308 56 375 8.82e-45 157
MsG0180001901.01.T01 MTR_5g072060 37.304 319 151 11 1 305 45 328 1.47e-44 160
MsG0180001901.01.T01 MTR_5g096910 36.860 293 136 12 35 308 88 350 2.24e-44 155
MsG0180001901.01.T01 MTR_6g087620 34.948 289 129 6 36 308 1 246 3.75e-44 152
MsG0180001901.01.T01 MTR_5g086590 41.485 229 109 6 82 308 12 217 1.24e-43 149
MsG0180001901.01.T01 MTR_5g076490 38.298 282 125 9 36 297 31 283 1.51e-43 152
MsG0180001901.01.T01 MTR_5g043605 35.740 277 132 7 46 308 54 298 4.57e-43 152
MsG0180001901.01.T01 MTR_1g053510 34.884 301 144 10 36 308 88 364 8.58e-43 152
MsG0180001901.01.T01 MTR_5g022850 36.426 291 148 10 36 308 41 312 9.56e-43 150
MsG0180001901.01.T01 MTR_4g028680 35.135 296 152 12 33 308 83 358 9.72e-43 152
MsG0180001901.01.T01 MTR_5g068490 36.398 261 128 7 45 303 8 232 1.41e-42 148
MsG0180001901.01.T01 MTR_5g092860 33.333 336 151 14 1 308 41 331 1.48e-42 150
MsG0180001901.01.T01 MTR_8g063350 34.266 286 144 6 34 305 43 298 1.62e-42 149
MsG0180001901.01.T01 MTR_6g090260 47.531 162 64 4 154 308 73 220 1.80e-42 147
MsG0180001901.01.T01 MTR_7g016930 36.237 287 140 8 1 281 55 304 2.84e-42 149
MsG0180001901.01.T01 MTR_6g004450 42.289 201 89 5 113 307 47 226 1.03e-41 145
MsG0180001901.01.T01 MTR_5g098950 39.301 229 90 6 85 308 90 274 4.79e-41 144
MsG0180001901.01.T01 MTR_1g098180 36.050 319 135 12 1 305 58 321 5.28e-41 146
MsG0180001901.01.T01 MTR_8g046200 34.770 348 140 15 16 308 65 380 6.39e-41 147
MsG0180001901.01.T01 MTR_7g111700 34.495 287 137 8 36 308 75 324 1.21e-40 145
MsG0180001901.01.T01 MTR_8g033000 32.945 343 171 11 1 308 3 321 4.44e-40 144
MsG0180001901.01.T01 MTR_8g032980 35.530 349 160 15 1 308 55 379 5.91e-40 145
MsG0180001901.01.T01 MTR_8g063370 31.646 316 171 9 1 305 63 344 2.85e-39 142
MsG0180001901.01.T01 MTR_7g023800 30.723 332 143 9 1 308 1 269 3.13e-39 140
MsG0180001901.01.T01 MTR_8g046310 34.877 324 147 17 1 305 55 333 6.81e-38 138
MsG0180001901.01.T01 MTR_2g021920 37.799 209 101 6 115 307 37 232 7.48e-38 135
MsG0180001901.01.T01 MTR_8g033110 32.647 340 175 14 1 307 53 371 1.85e-37 138
MsG0180001901.01.T01 MTR_8g063310 31.915 329 177 12 5 305 41 350 1.93e-37 137
MsG0180001901.01.T01 MTR_5g091140 33.538 325 161 14 1 308 87 373 2.21e-37 138
MsG0180001901.01.T01 MTR_4g106530 49.640 139 22 1 94 232 1 91 4.99e-37 127
MsG0180001901.01.T01 MTR_5g074850 33.446 296 145 12 39 305 107 379 9.11e-37 140
MsG0180001901.01.T01 MTR_5g097850 43.114 167 85 3 144 308 46 204 2.82e-36 131
MsG0180001901.01.T01 MTR_5g023970 44.444 171 67 6 145 308 4 153 8.16e-36 128
MsG0180001901.01.T01 MTR_5g092850 34.114 299 146 13 37 308 84 358 3.27e-35 132
MsG0180001901.01.T01 MTR_6g009350 37.143 210 87 6 115 307 8 189 1.08e-34 125
MsG0180001901.01.T01 MTR_8g064790 33.803 284 109 9 34 308 82 295 7.16e-34 127
MsG0180001901.01.T01 MTR_8g046220 32.743 339 161 13 1 305 56 361 8.90e-34 128
MsG0180001901.01.T01 MTR_5g075370 31.366 322 162 13 15 305 62 355 1.32e-33 130
MsG0180001901.01.T01 MTR_5g097540 42.675 157 72 4 153 308 94 233 2.37e-31 119
MsG0180001901.01.T01 MTR_5g096900 33.779 299 136 13 1 273 51 313 1.10e-30 118
MsG0180001901.01.T01 MTR_3g018750 34.272 213 99 5 114 305 2 194 2.67e-30 115
MsG0180001901.01.T01 MTR_8g033090 31.065 338 181 14 1 307 53 369 6.21e-30 117
MsG0180001901.01.T01 MTR_5g094350 36.923 195 97 6 48 218 81 273 7.70e-30 114
MsG0180001901.01.T01 MTR_4g014370 39.506 162 69 4 156 308 154 295 1.79e-29 115
MsG0180001901.01.T01 MTR_8g035690 33.466 251 111 9 89 305 30 258 2.99e-29 114
MsG0180001901.01.T01 MTR_8g018110 29.970 337 171 12 1 307 53 354 4.62e-29 115
MsG0180001901.01.T01 MTR_2g104260 34.335 233 128 10 1 212 49 277 5.71e-27 107
MsG0180001901.01.T01 MTR_5g097820 31.797 217 126 7 1 204 62 269 6.21e-26 103
MsG0180001901.01.T01 MTR_3g030620 44.697 132 60 3 179 308 19 139 6.42e-26 101
MsG0180001901.01.T01 MTR_1g050308 34.272 213 122 6 1 208 73 272 8.15e-26 105
MsG0180001901.01.T01 MTR_1g050308 54.930 71 28 2 238 306 238 306 4.72e-15 75.1
MsG0180001901.01.T01 MTR_7g017150 37.662 154 76 4 151 299 77 215 2.37e-23 97.1
MsG0180001901.01.T01 MTR_7g017120 37.662 154 76 4 151 299 76 214 3.88e-23 96.7
MsG0180001901.01.T01 MTR_7g101200 29.832 238 105 10 84 307 39 228 1.82e-22 94.0
MsG0180001901.01.T01 MTR_7g104710 28.622 283 132 10 1 265 56 286 1.86e-22 95.5
MsG0180001901.01.T01 MTR_6g079730 45.872 109 45 2 200 308 14 108 2.10e-22 91.7
MsG0180001901.01.T01 MTR_5g066420 38.931 131 62 2 183 308 1 118 2.06e-21 89.0
MsG0180001901.01.T01 MTR_1g076810 33.668 199 99 10 1 182 57 239 1.02e-19 89.7
MsG0180001901.01.T01 MTR_5g037190 30.556 216 118 7 1 205 55 249 1.86e-18 83.6
MsG0180001901.01.T01 MTR_6g070880 33.838 198 100 10 1 176 71 259 8.63e-18 81.6
MsG0180001901.01.T01 MTR_6g071420 33.838 198 100 10 1 176 71 259 8.63e-18 81.6
MsG0180001901.01.T01 MTR_5g036250 53.623 69 32 0 240 308 2 70 8.74e-18 80.1
MsG0180001901.01.T01 MTR_5g096920 37.795 127 61 4 183 308 1 110 1.36e-17 78.6
MsG0180001901.01.T01 MTR_5g096050 38.525 122 49 2 183 292 1 108 2.07e-17 77.0
MsG0180001901.01.T01 MTR_1g047080 33.184 223 122 11 1 218 3 203 5.39e-17 80.1
MsG0180001901.01.T01 MTR_8g073570 36.508 126 76 2 1 126 49 170 5.80e-17 77.4
MsG0180001901.01.T01 MTR_5g097660 56.250 64 27 1 246 308 15 78 1.36e-16 75.1
MsG0180001901.01.T01 MTR_1g115205 34.940 166 74 7 42 191 90 237 1.45e-16 78.2
MsG0180001901.01.T01 MTR_2g022020 50.000 64 32 0 245 308 17 80 1.12e-15 72.4
MsG0180001901.01.T01 MTR_3g047540 47.143 70 33 2 152 218 2 70 2.09e-15 70.9
MsG0180001901.01.T01 MTR_1g050305 56.164 73 23 3 242 305 8 80 8.05e-15 70.9
MsG0180001901.01.T01 MTR_1g097330 28.700 223 120 8 68 285 109 297 1.10e-14 74.3
MsG0180001901.01.T01 MTR_5g086780 49.206 63 32 0 246 308 148 210 1.21e-13 69.3
MsG0180001901.01.T01 MTR_4g094400 35.294 119 48 4 180 283 7 111 1.83e-12 63.9
MsG0180001901.01.T01 MTR_4g133170 25.591 254 138 12 68 302 90 311 3.77e-12 66.6
MsG0180001901.01.T01 MTR_4g023130 25.652 230 120 8 84 303 77 265 5.16e-12 65.9
MsG0180001901.01.T01 MTR_3g007360 24.904 261 132 9 55 286 114 339 1.39e-11 65.1
MsG0180001901.01.T01 MTR_3g007410 24.904 261 132 9 55 286 114 339 2.43e-11 64.3
MsG0180001901.01.T01 MTR_2g024100 25.523 239 132 11 84 302 120 332 3.29e-11 63.9
MsG0180001901.01.T01 MTR_7g407070 28.261 230 126 12 68 285 135 337 3.67e-11 63.9
MsG0180001901.01.T01 MTR_3g006670 26.016 246 141 10 66 304 144 355 4.29e-11 63.5
MsG0180001901.01.T01 MTR_4g023080 25.652 230 120 8 84 303 118 306 5.45e-11 63.2
MsG0180001901.01.T01 MTR_0115s0020 25.207 242 147 9 2 222 46 274 6.63e-11 63.2
MsG0180001901.01.T01 MTR_3g083010 23.853 327 173 15 5 285 25 321 9.17e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 65 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATATCTCCTTTATATTT+AGG 0.181614 1:+29000711 None:intergenic
ATCTTATGGTCCTCATTAAA+AGG 0.186737 1:+29000410 None:intergenic
CAACAACACCACGCCCCTTT+TGG 0.226271 1:-29000325 MsG0180001901.01.T01:CDS
TTTGACTTGGTGGTGTTAAA+TGG 0.231992 1:-29000041 MsG0180001901.01.T01:CDS
ACCATCGATTTGTTGCATTA+TGG 0.239651 1:-29000532 MsG0180001901.01.T01:CDS
TATACTGATATGTTGGATTC+TGG 0.248324 1:-29000587 MsG0180001901.01.T01:CDS
CTCTTGGAAGAGACTTGATT+TGG 0.309894 1:-29000279 MsG0180001901.01.T01:CDS
CCGATACACAGATATGAATT+TGG 0.312032 1:+29000760 None:intergenic
ACCTTACGACCTATACTTAT+AGG 0.331048 1:+29000251 None:intergenic
TAACCTGACGAATGATCTTA+TGG 0.335462 1:+29000396 None:intergenic
TGAAAACAAACAGTATAAAA+AGG 0.337469 1:+29000455 None:intergenic
TGACGACAAATTTGAGAATA+GGG 0.341369 1:-29000651 MsG0180001901.01.T01:CDS
GAATCCAGCTACTAAGGAAT+TGG 0.351870 1:-29000510 MsG0180001901.01.T01:CDS
TTGACGACAAATTTGAGAAT+AGG 0.356053 1:-29000652 MsG0180001901.01.T01:CDS
CAAAAGGGGCGTGGTGTTGT+TGG 0.388016 1:+29000326 None:intergenic
TCATCCTGTTCAAATGAAAC+TGG 0.394027 1:+29000614 None:intergenic
ATGGCCAGTTTCATTTGAAC+AGG 0.401757 1:-29000618 MsG0180001901.01.T01:CDS
ACTTGAATGGATTGTGTCAT+TGG 0.406392 1:-29000226 MsG0180001901.01.T01:CDS
ATATCTCTTCCTAAATATAA+AGG 0.411065 1:-29000720 MsG0180001901.01.T01:CDS
AATCTGATGACAACGTTGAT+TGG 0.411621 1:-29000358 MsG0180001901.01.T01:CDS
TACACTATAAATCTCCCAAA+AGG 0.416151 1:+29000310 None:intergenic
TGAGATTTATACTGATATGT+TGG 0.429386 1:-29000594 MsG0180001901.01.T01:CDS
GTTTCCAATTCCTTAGTAGC+TGG 0.429694 1:+29000506 None:intergenic
TGGACTAAACTCTTCATTGT+TGG 0.431221 1:-28999936 MsG0180001901.01.T01:CDS
TTGACTTGGTGGTGTTAAAT+GGG 0.433750 1:-29000040 MsG0180001901.01.T01:CDS
AAAAGGGGCGTGGTGTTGTT+GGG 0.449466 1:+29000327 None:intergenic
CTTTACTACACATTTACCCT+TGG 0.456076 1:-29000141 MsG0180001901.01.T01:CDS
ACACTATAAATCTCCCAAAA+GGG 0.461907 1:+29000311 None:intergenic
AACTTGGTGTTAGCGAATCA+TGG 0.472503 1:-28999956 MsG0180001901.01.T01:CDS
GGTCGTAAGGTGTACTTGAA+TGG 0.473243 1:-29000239 MsG0180001901.01.T01:CDS
CTCCTTAGTACCCACTCATC+AGG 0.480423 1:+29000101 None:intergenic
ATAGTGTAAGAAGTAACTCT+TGG 0.480890 1:-29000295 MsG0180001901.01.T01:CDS
ATTATGGAATCCAGCTACTA+AGG 0.481458 1:-29000516 MsG0180001901.01.T01:CDS
GATCTCAAAGCATGTAAAGA+TGG 0.482324 1:-29000006 MsG0180001901.01.T01:CDS
CCAACAGGACGCTCCACGTT+AGG 0.487448 1:+28999906 None:intergenic
TTGGATATGCCTATAAGTAT+AGG 0.496993 1:-29000260 MsG0180001901.01.T01:CDS
GCCTATAAGTATAGGTCGTA+AGG 0.510291 1:-29000252 MsG0180001901.01.T01:CDS
AAGGTGACAATGATGATAAG+TGG 0.514461 1:-29000076 MsG0180001901.01.T01:CDS
GTGGAGCGTCCTGTTGGAGC+AGG 0.515160 1:-28999900 MsG0180001901.01.T01:CDS
GGACCATAAGATCATTCGTC+AGG 0.516916 1:-29000399 MsG0180001901.01.T01:CDS
TCAGAACCATCTCGTGGATG+CGG 0.523503 1:+29000689 None:intergenic
ACATAGATACTTTGACTTGG+TGG 0.526466 1:-29000051 MsG0180001901.01.T01:CDS
TCTTATGGTCCTCATTAAAA+GGG 0.533003 1:+29000411 None:intergenic
ATCCTGATGAGTGGGTACTA+AGG 0.535030 1:-29000103 MsG0180001901.01.T01:CDS
TGAATGGATTGTGTCATTGG+TGG 0.536839 1:-29000223 MsG0180001901.01.T01:CDS
GAATGTAGCTGATGCATACA+TGG 0.537151 1:-29000192 MsG0180001901.01.T01:CDS
GAGTGGGTACTAAGGAGAGA+AGG 0.539541 1:-29000095 MsG0180001901.01.T01:CDS
ATAGCTATCTTGCATATCCA+AGG 0.540851 1:+29000124 None:intergenic
CCTAACGTGGAGCGTCCTGT+TGG 0.542213 1:-28999906 MsG0180001901.01.T01:CDS
TGTTGGACCCTTGCCTAACG+TGG 0.554900 1:-28999919 MsG0180001901.01.T01:CDS
CCAAATTCATATCTGTGTAT+CGG 0.567612 1:-29000760 MsG0180001901.01.T01:CDS
GAATGGATTGTGTCATTGGT+GGG 0.574493 1:-29000222 MsG0180001901.01.T01:CDS
AGGACGCTCCACGTTAGGCA+AGG 0.578365 1:+28999911 None:intergenic
TCCATAATGCAACAAATCGA+TGG 0.582817 1:+29000531 None:intergenic
TAAATCTCCCAAAAGGGGCG+TGG 0.598439 1:+29000317 None:intergenic
CACTATAAATCTCCCAAAAG+GGG 0.600870 1:+29000312 None:intergenic
TACAAATCAGAACCATCTCG+TGG 0.610841 1:+29000683 None:intergenic
AGGTGACAATGATGATAAGT+GGG 0.615556 1:-29000075 MsG0180001901.01.T01:CDS
GGACGCTCCACGTTAGGCAA+GGG 0.621730 1:+28999912 None:intergenic
AAGATAGCTATCCTGATGAG+TGG 0.630767 1:-29000112 MsG0180001901.01.T01:CDS
ATATTGCCGCATCCACGAGA+TGG 0.631328 1:-29000695 MsG0180001901.01.T01:CDS
AGATAGCTATCCTGATGAGT+GGG 0.641843 1:-29000111 MsG0180001901.01.T01:CDS
TAGCTATCTTGCATATCCAA+GGG 0.642438 1:+29000125 None:intergenic
GGTACATAGATACTTTGACT+TGG 0.658683 1:-29000054 MsG0180001901.01.T01:CDS
ACACAGATATGAATTTGGTG+CGG 0.715710 1:+29000765 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATCTCTTCCTAAATATAA+AGG - Chr1:28999916-28999935 MsG0180001901.01.T01:CDS 20.0%
!! TGAAAACAAACAGTATAAAA+AGG + Chr1:29000184-29000203 None:intergenic 20.0%
! GCAATATCTCCTTTATATTT+AGG + Chr1:28999928-28999947 None:intergenic 25.0%
! TGAGATTTATACTGATATGT+TGG - Chr1:29000042-29000061 MsG0180001901.01.T01:CDS 25.0%
!! TCTTTTCACATCTATGTTTT+GGG - Chr1:29000655-29000674 MsG0180001901.01.T01:CDS 25.0%
!! TTCTTTTCACATCTATGTTT+TGG - Chr1:29000654-29000673 MsG0180001901.01.T01:CDS 25.0%
AAAAAGGTAAAAACTCAGCT+GGG + Chr1:29000168-29000187 None:intergenic 30.0%
ACACTATAAATCTCCCAAAA+GGG + Chr1:29000328-29000347 None:intergenic 30.0%
ATAGTGTAAGAAGTAACTCT+TGG - Chr1:29000341-29000360 MsG0180001901.01.T01:CDS 30.0%
ATCTTATGGTCCTCATTAAA+AGG + Chr1:29000229-29000248 None:intergenic 30.0%
CCAAATTCATATCTGTGTAT+CGG - Chr1:28999876-28999895 MsG0180001901.01.T01:CDS 30.0%
TAAAAAGGTAAAAACTCAGC+TGG + Chr1:29000169-29000188 None:intergenic 30.0%
TACACTATAAATCTCCCAAA+AGG + Chr1:29000329-29000348 None:intergenic 30.0%
TATACTGATATGTTGGATTC+TGG - Chr1:29000049-29000068 MsG0180001901.01.T01:CDS 30.0%
TCTTATGGTCCTCATTAAAA+GGG + Chr1:29000228-29000247 None:intergenic 30.0%
TGACGACAAATTTGAGAATA+GGG - Chr1:28999985-29000004 MsG0180001901.01.T01:CDS 30.0%
TTGACGACAAATTTGAGAAT+AGG - Chr1:28999984-29000003 MsG0180001901.01.T01:CDS 30.0%
TTGGATATGCCTATAAGTAT+AGG - Chr1:29000376-29000395 MsG0180001901.01.T01:CDS 30.0%
! TGTTTTCATCTTCATGGATA+TGG - Chr1:29000196-29000215 MsG0180001901.01.T01:CDS 30.0%
!! ACTGTTTGTTTTCATCTTCA+TGG - Chr1:29000190-29000209 MsG0180001901.01.T01:CDS 30.0%
!! AGAATAGGGTCTTTTTGAAA+TGG - Chr1:28999999-29000018 MsG0180001901.01.T01:CDS 30.0%
AAGGTGACAATGATGATAAG+TGG - Chr1:29000560-29000579 MsG0180001901.01.T01:CDS 35.0%
AATCTGATGACAACGTTGAT+TGG - Chr1:29000278-29000297 MsG0180001901.01.T01:CDS 35.0%
ACCTTACGACCTATACTTAT+AGG + Chr1:29000388-29000407 None:intergenic 35.0%
AGGTGACAATGATGATAAGT+GGG - Chr1:29000561-29000580 MsG0180001901.01.T01:CDS 35.0%
ATAGCTATCTTGCATATCCA+AGG + Chr1:29000515-29000534 None:intergenic 35.0%
ATTATGGAATCCAGCTACTA+AGG - Chr1:29000120-29000139 MsG0180001901.01.T01:CDS 35.0%
CACTATAAATCTCCCAAAAG+GGG + Chr1:29000327-29000346 None:intergenic 35.0%
CCGATACACAGATATGAATT+TGG + Chr1:28999879-28999898 None:intergenic 35.0%
CTTTACTACACATTTACCCT+TGG - Chr1:29000495-29000514 MsG0180001901.01.T01:CDS 35.0%
GATCTCAAAGCATGTAAAGA+TGG - Chr1:29000630-29000649 MsG0180001901.01.T01:CDS 35.0%
GGTACATAGATACTTTGACT+TGG - Chr1:29000582-29000601 MsG0180001901.01.T01:CDS 35.0%
TAACCTGACGAATGATCTTA+TGG + Chr1:29000243-29000262 None:intergenic 35.0%
TAGCTATCTTGCATATCCAA+GGG + Chr1:29000514-29000533 None:intergenic 35.0%
TCATCCTGTTCAAATGAAAC+TGG + Chr1:29000025-29000044 None:intergenic 35.0%
TCCATAATGCAACAAATCGA+TGG + Chr1:29000108-29000127 None:intergenic 35.0%
TGGACTAAACTCTTCATTGT+TGG - Chr1:29000700-29000719 MsG0180001901.01.T01:CDS 35.0%
! ACACAGATATGAATTTGGTG+CGG + Chr1:28999874-28999893 None:intergenic 35.0%
! TGGTTATGACCCTTTTAATG+AGG - Chr1:29000216-29000235 MsG0180001901.01.T01:CDS 35.0%
!! ACATAGATACTTTGACTTGG+TGG - Chr1:29000585-29000604 MsG0180001901.01.T01:CDS 35.0%
!! ACCATCGATTTGTTGCATTA+TGG - Chr1:29000104-29000123 MsG0180001901.01.T01:CDS 35.0%
!! ACTTGAATGGATTGTGTCAT+TGG - Chr1:29000410-29000429 MsG0180001901.01.T01:CDS 35.0%
!! ATCTATGTTTTGGGTGAACT+TGG - Chr1:29000664-29000683 MsG0180001901.01.T01:CDS 35.0%
!! TTGACTTGGTGGTGTTAAAT+GGG - Chr1:29000596-29000615 MsG0180001901.01.T01:CDS 35.0%
!! TTTGACTTGGTGGTGTTAAA+TGG - Chr1:29000595-29000614 MsG0180001901.01.T01:CDS 35.0%
AAGATAGCTATCCTGATGAG+TGG - Chr1:29000524-29000543 MsG0180001901.01.T01:CDS 40.0%
AGATAGCTATCCTGATGAGT+GGG - Chr1:29000525-29000544 MsG0180001901.01.T01:CDS 40.0%
ATGGCCAGTTTCATTTGAAC+AGG - Chr1:29000018-29000037 MsG0180001901.01.T01:CDS 40.0%
CTCTTGGAAGAGACTTGATT+TGG - Chr1:29000357-29000376 MsG0180001901.01.T01:CDS 40.0%
GAATCCAGCTACTAAGGAAT+TGG - Chr1:29000126-29000145 MsG0180001901.01.T01:CDS 40.0%
GAATGTAGCTGATGCATACA+TGG - Chr1:29000444-29000463 MsG0180001901.01.T01:CDS 40.0%
GCCTATAAGTATAGGTCGTA+AGG - Chr1:29000384-29000403 MsG0180001901.01.T01:CDS 40.0%
GTTTCCAATTCCTTAGTAGC+TGG + Chr1:29000133-29000152 None:intergenic 40.0%
TACAAATCAGAACCATCTCG+TGG + Chr1:28999956-28999975 None:intergenic 40.0%
!! AACTTGGTGTTAGCGAATCA+TGG - Chr1:29000680-29000699 MsG0180001901.01.T01:CDS 40.0%
!! GAATGGATTGTGTCATTGGT+GGG - Chr1:29000414-29000433 MsG0180001901.01.T01:CDS 40.0%
!! TGAATGGATTGTGTCATTGG+TGG - Chr1:29000413-29000432 MsG0180001901.01.T01:CDS 40.0%
ATCCTGATGAGTGGGTACTA+AGG - Chr1:29000533-29000552 MsG0180001901.01.T01:CDS 45.0%
CCTGTTGGAGCAGGAAAAAA+AGG - Chr1:29000745-29000764 MsG0180001901.01.T01:CDS 45.0%
GGACCATAAGATCATTCGTC+AGG - Chr1:29000237-29000256 MsG0180001901.01.T01:CDS 45.0%
GGTCGTAAGGTGTACTTGAA+TGG - Chr1:29000397-29000416 MsG0180001901.01.T01:CDS 45.0%
!!! CCTTTTTTTCCTGCTCCAAC+AGG + Chr1:29000748-29000767 None:intergenic 45.0%
ATATTGCCGCATCCACGAGA+TGG - Chr1:28999941-28999960 MsG0180001901.01.T01:CDS 50.0%
CTCCTTAGTACCCACTCATC+AGG + Chr1:29000538-29000557 None:intergenic 50.0%
GAGTGGGTACTAAGGAGAGA+AGG - Chr1:29000541-29000560 MsG0180001901.01.T01:CDS 50.0%
GGTAAAAACTCAGCTGGGCT+AGG + Chr1:29000163-29000182 None:intergenic 50.0%
TAAATCTCCCAAAAGGGGCG+TGG + Chr1:29000322-29000341 None:intergenic 50.0%
TCAGAACCATCTCGTGGATG+CGG + Chr1:28999950-28999969 None:intergenic 50.0%
! AAAAACTCAGCTGGGCTAGG+CGG + Chr1:29000160-29000179 None:intergenic 50.0%
! AACAACACCACGCCCCTTTT+GGG - Chr1:29000312-29000331 MsG0180001901.01.T01:CDS 50.0%
!! AAAAGGGGCGTGGTGTTGTT+GGG + Chr1:29000312-29000331 None:intergenic 50.0%
TGTTGGACCCTTGCCTAACG+TGG - Chr1:29000717-29000736 MsG0180001901.01.T01:CDS 55.0%
! CAACAACACCACGCCCCTTT+TGG - Chr1:29000311-29000330 MsG0180001901.01.T01:CDS 55.0%
!! CAAAAGGGGCGTGGTGTTGT+TGG + Chr1:29000313-29000332 None:intergenic 55.0%
AGGACGCTCCACGTTAGGCA+AGG + Chr1:29000728-29000747 None:intergenic 60.0%
CCAACAGGACGCTCCACGTT+AGG + Chr1:29000733-29000752 None:intergenic 60.0%
GGACGCTCCACGTTAGGCAA+GGG + Chr1:29000727-29000746 None:intergenic 60.0%
! CCTAACGTGGAGCGTCCTGT+TGG - Chr1:29000730-29000749 MsG0180001901.01.T01:CDS 60.0%
! GTGGAGCGTCCTGTTGGAGC+AGG - Chr1:29000736-29000755 MsG0180001901.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 28999866 29000792 28999866 ID=MsG0180001901.01;Name=MsG0180001901.01
Chr1 mRNA 28999866 29000792 28999866 ID=MsG0180001901.01.T01;Parent=MsG0180001901.01;Name=MsG0180001901.01.T01;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|308
Chr1 exon 28999866 29000792 28999866 ID=MsG0180001901.01.T01:exon:18903;Parent=MsG0180001901.01.T01
Chr1 CDS 28999866 29000792 28999866 ID=MsG0180001901.01.T01:cds;Parent=MsG0180001901.01.T01
Gene Sequence

>MsG0180001901.01.T01

ATGTTCCGCACCAAATTCATATCTGTGTATCGGTCATTGTATAATGACACATATCTCTTCCTAAATATAAAGGAGATATTGCCGCATCCACGAGATGGTTCTGATTTGTATTTGCTCTTTGACGACAAATTTGAGAATAGGGTCTTTTTGAAATGGCCAGTTTCATTTGAACAGGATGAGATTTATACTGATATGTTGGATTCTGGTATTAATGACGTTCTTTGTTTCTCTGATCTTAACCATCGATTTGTTGCATTATGGAATCCAGCTACTAAGGAATTGGAAACTATTCCGCCTAGCCCAGCTGAGTTTTTACCTTTTTATACTGTTTGTTTTCATCTTCATGGATATGGTTATGACCCTTTTAATGAGGACCATAAGATCATTCGTCAGGTTAAAGTTAGTAAATATAAATCTGATGACAACGTTGATTGGAATTATCTCCCAACAACACCACGCCCCTTTTGGGAGATTTATAGTGTAAGAAGTAACTCTTGGAAGAGACTTGATTTGGATATGCCTATAAGTATAGGTCGTAAGGTGTACTTGAATGGATTGTGTCATTGGTGGGCTAATACGAATGTAGCTGATGCATACATGGTTTCTTTTGACTTGAGAAATGAGAACTTCTTTACTACACATTTACCCTTGGATATGCAAGATAGCTATCCTGATGAGTGGGTACTAAGGAGAGAAGGTGACAATGATGATAAGTGGGTACATAGATACTTTGACTTGGTGGTGTTAAATGGGTTCGTTGCTATGATCTCAAAGCATGTAAAGATGGCTTCTTTTCACATCTATGTTTTGGGTGAACTTGGTGTTAGCGAATCATGGACTAAACTCTTCATTGTTGGACCCTTGCCTAACGTGGAGCGTCCTGTTGGAGCAGGAAAAAAAGGTGATATATTCTTTATAAAAAAATGA
Protein sequence

>MsG0180001901.01.T01

MFRTKFISVYRSLYNDTYLFLNIKEILPHPRDGSDLYLLFDDKFENRVFLKWPVSFEQDEIYTDMLDSGINDVLCFSDLNHRFVALWNPATKELETIPPSPAEFLPFYTVCFHLHGYGYDPFNEDHKIIRQVKVSKYKSDDNVDWNYLPTTPRPFWEIYSVRSNSWKRLDLDMPISIGRKVYLNGLCHWWANTNVADAYMVSFDLRNENFFTTHLPLDMQDSYPDEWVLRREGDNDDKWVHRYFDLVVLNGFVAMISKHVKMASFHIYVLGELGVSESWTKLFIVGPLPNVERPVGAGKKGDIFFIKK*