AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580026166.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026166.01.T01 MTR_4g007940 54.907 377 100 7 60 401 1 342 2.68e-120 353
MsG0580026166.01.T01 MTR_0831s0010 47.529 425 150 5 5 400 41 421 8.16e-116 344
MsG0580026166.01.T01 MTR_8g063330 49.872 391 117 8 1 355 1 348 6.58e-104 315
MsG0580026166.01.T01 MTR_0129s0070 34.783 437 194 9 6 401 11 397 1.57e-73 235
MsG0580026166.01.T01 MTR_0129s0120 35.172 435 191 9 6 399 11 395 7.07e-73 233
MsG0580026166.01.T01 MTR_8g464770 36.930 417 181 12 7 392 7 372 6.02e-69 222
MsG0580026166.01.T01 MTR_3g011120 37.383 428 170 12 1 389 1 369 7.84e-68 219
MsG0580026166.01.T01 MTR_2g066170 36.425 442 185 13 1 401 1 387 5.35e-67 218
MsG0580026166.01.T01 MTR_2g021770 33.480 454 203 13 1 401 1 408 5.31e-65 213
MsG0580026166.01.T01 MTR_6g080680 36.195 431 175 16 4 390 11 385 2.26e-64 211
MsG0580026166.01.T01 MTR_6g086825 34.773 440 206 13 4 401 61 461 1.89e-63 211
MsG0580026166.01.T01 MTR_3g019290 55.224 201 60 2 1 200 1 172 2.82e-62 198
MsG0580026166.01.T01 MTR_6g090180 34.467 441 180 16 5 389 40 427 3.27e-62 207
MsG0580026166.01.T01 MTR_2g066200 35.281 445 188 13 1 401 1 389 3.28e-62 205
MsG0580026166.01.T01 MTR_8g064890 34.615 442 186 13 4 401 7 389 4.23e-62 205
MsG0580026166.01.T01 MTR_2g034700 36.364 429 182 13 3 398 4 374 1.42e-60 201
MsG0580026166.01.T01 MTR_8g012090 37.726 387 154 13 7 369 11 334 1.04e-59 197
MsG0580026166.01.T01 MTR_2g021800 31.304 460 206 13 3 401 7 417 2.28e-59 199
MsG0580026166.01.T01 MTR_2g066150 35.714 420 192 11 1 401 1 361 3.06e-59 197
MsG0580026166.01.T01 MTR_0129s0050 34.452 447 179 16 4 390 6 398 3.77e-59 198
MsG0580026166.01.T01 MTR_6g478230 34.033 429 208 13 7 401 21 408 7.12e-59 197
MsG0580026166.01.T01 MTR_8g467670 33.410 437 198 11 1 401 1 380 1.20e-58 196
MsG0580026166.01.T01 MTR_8g064790 35.181 415 178 12 1 401 1 338 4.29e-58 193
MsG0580026166.01.T01 MTR_7g079640 36.111 396 177 13 12 381 12 357 1.10e-57 193
MsG0580026166.01.T01 MTR_2g104340 34.579 428 177 18 12 390 13 386 1.51e-57 193
MsG0580026166.01.T01 MTR_5g097330 33.259 451 196 15 1 399 5 402 3.78e-57 192
MsG0580026166.01.T01 MTR_4g016000 46.531 245 49 4 132 350 2 190 4.34e-57 186
MsG0580026166.01.T01 MTR_2g022000 32.569 436 187 14 3 378 7 395 5.89e-57 193
MsG0580026166.01.T01 MTR_5g097220 37.374 396 194 13 13 378 13 384 2.39e-56 191
MsG0580026166.01.T01 MTR_2g065860 34.152 448 189 13 1 401 1 389 4.36e-56 189
MsG0580026166.01.T01 MTR_8g063440 33.333 447 189 13 1 401 1 384 1.10e-55 188
MsG0580026166.01.T01 MTR_5g037680 33.810 420 193 11 9 390 4 376 5.57e-55 188
MsG0580026166.01.T01 MTR_8g046310 35.417 432 180 15 4 398 7 376 7.84e-55 186
MsG0580026166.01.T01 MTR_0134s0060 32.527 455 193 15 1 392 1 404 1.59e-54 186
MsG0580026166.01.T01 MTR_0054s0090 32.731 443 194 13 12 401 16 407 2.46e-54 185
MsG0580026166.01.T01 MTR_6g079810 31.390 446 204 11 5 396 62 459 3.03e-54 186
MsG0580026166.01.T01 MTR_8g039500 36.430 409 157 14 1 369 1 346 3.97e-54 183
MsG0580026166.01.T01 MTR_7g069550 34.988 423 181 18 7 383 9 383 1.25e-53 183
MsG0580026166.01.T01 MTR_2g065840 34.753 446 188 17 1 400 1 389 1.52e-53 182
MsG0580026166.01.T01 MTR_4g055820 35.176 398 165 15 6 356 8 359 3.61e-53 181
MsG0580026166.01.T01 MTR_4g028510 33.483 445 199 15 3 399 6 401 2.11e-52 181
MsG0580026166.01.T01 MTR_1g007450 34.954 432 183 15 1 385 1 381 4.77e-52 179
MsG0580026166.01.T01 MTR_2g038680 32.456 456 185 16 5 395 9 406 6.34e-52 179
MsG0580026166.01.T01 MTR_6g079770 31.507 438 195 13 12 390 16 407 1.60e-51 178
MsG0580026166.01.T01 MTR_4g028660 33.568 426 181 16 3 379 6 378 1.96e-51 177
MsG0580026166.01.T01 MTR_4g027450 33.568 426 181 16 3 379 6 378 1.96e-51 177
MsG0580026166.01.T01 MTR_5g024260 34.762 420 181 14 4 378 13 384 2.57e-51 177
MsG0580026166.01.T01 MTR_1g098180 31.504 419 196 11 4 396 9 362 2.87e-51 176
MsG0580026166.01.T01 MTR_4g117550 30.986 426 180 11 6 390 11 363 8.25e-51 175
MsG0580026166.01.T01 MTR_5g097680 31.965 463 201 16 5 399 10 426 1.22e-50 176
MsG0580026166.01.T01 MTR_5g069390 31.236 445 212 12 2 396 13 413 1.41e-50 177
MsG0580026166.01.T01 MTR_8g032980 32.473 465 193 14 7 401 9 422 2.05e-50 175
MsG0580026166.01.T01 MTR_4g130330 32.127 442 195 15 9 399 3 390 3.42e-50 174
MsG0580026166.01.T01 MTR_7g017040 31.674 442 204 11 4 399 8 397 5.44e-50 174
MsG0580026166.01.T01 MTR_4g014370 31.236 461 196 16 7 401 5 410 1.33e-49 173
MsG0580026166.01.T01 MTR_5g072060 36.020 397 177 16 12 380 5 352 1.42e-49 177
MsG0580026166.01.T01 MTR_5g059430 31.544 447 207 11 7 397 20 423 4.73e-49 172
MsG0580026166.01.T01 MTR_4g028750 33.017 421 187 14 4 379 7 377 6.73e-49 171
MsG0580026166.01.T01 MTR_4g102410 32.207 444 200 16 5 397 54 447 1.10e-48 171
MsG0580026166.01.T01 MTR_8g036970 34.430 395 162 15 17 392 2 318 1.28e-48 169
MsG0580026166.01.T01 MTR_8g046200 34.228 447 209 18 4 398 7 420 1.31e-48 171
MsG0580026166.01.T01 MTR_4g028680 32.889 450 197 17 3 401 6 401 3.27e-48 169
MsG0580026166.01.T01 MTR_1g007410 31.973 441 194 16 5 394 15 400 4.04e-48 169
MsG0580026166.01.T01 MTR_8g046220 32.189 466 175 13 6 398 7 404 5.55e-48 168
MsG0580026166.01.T01 MTR_1g053510 32.009 453 190 16 5 396 7 402 1.36e-47 167
MsG0580026166.01.T01 MTR_2g064950 33.019 424 170 15 12 381 9 372 1.75e-47 167
MsG0580026166.01.T01 MTR_7g017130 31.481 432 198 9 13 399 18 396 2.90e-47 166
MsG0580026166.01.T01 MTR_7g017170 32.195 410 180 9 13 377 18 374 2.92e-46 164
MsG0580026166.01.T01 MTR_8g033090 31.628 430 229 15 8 399 9 411 4.67e-46 163
MsG0580026166.01.T01 MTR_7g111700 32.779 421 184 15 12 401 14 366 5.26e-46 162
MsG0580026166.01.T01 MTR_8g013000 34.359 390 166 10 12 357 9 352 2.04e-45 162
MsG0580026166.01.T01 MTR_5g096630 31.264 451 205 15 6 399 11 413 2.91e-45 161
MsG0580026166.01.T01 MTR_6g461660 32.353 442 196 16 5 398 12 398 1.04e-44 159
MsG0580026166.01.T01 MTR_8g041270 30.909 440 202 14 4 390 6 396 4.90e-44 158
MsG0580026166.01.T01 MTR_5g096910 30.664 437 204 15 7 399 11 392 8.68e-44 157
MsG0580026166.01.T01 MTR_8g033110 31.429 455 200 15 8 399 9 414 4.56e-43 155
MsG0580026166.01.T01 MTR_4g014370 31.387 411 178 15 7 401 16 338 2.55e-42 152
MsG0580026166.01.T01 MTR_8g018110 32.067 421 224 14 8 399 9 396 7.24e-42 152
MsG0580026166.01.T01 MTR_5g097350 29.723 397 184 13 22 378 25 366 1.23e-41 151
MsG0580026166.01.T01 MTR_2g021950 28.375 437 201 16 12 395 15 392 1.13e-39 146
MsG0580026166.01.T01 MTR_6g090250 29.291 437 197 14 31 398 29 422 1.38e-39 146
MsG0580026166.01.T01 MTR_2g104280 31.954 435 188 18 12 390 13 395 1.63e-39 145
MsG0580026166.01.T01 MTR_5g091140 30.493 446 180 16 12 401 47 418 3.53e-39 145
MsG0580026166.01.T01 MTR_5g043605 30.488 410 168 13 12 399 24 338 5.96e-39 143
MsG0580026166.01.T01 MTR_8g063350 28.046 435 181 11 2 398 3 343 1.01e-38 142
MsG0580026166.01.T01 MTR_0054s0100 29.039 458 206 14 5 401 10 409 2.51e-38 142
MsG0580026166.01.T01 MTR_7g019250 37.917 240 118 4 139 377 204 413 3.30e-38 144
MsG0580026166.01.T01 MTR_6g078010 30.319 376 186 13 50 395 1 330 1.60e-37 139
MsG0580026166.01.T01 MTR_1g007430 33.243 370 166 11 24 361 7 327 2.19e-37 138
MsG0580026166.01.T01 MTR_8g063310 27.169 438 229 14 2 400 11 397 8.51e-37 138
MsG0580026166.01.T01 MTR_8g063370 28.770 431 180 17 2 379 17 373 3.26e-36 137
MsG0580026166.01.T01 MTR_5g074850 29.252 441 192 19 1 380 23 404 5.00e-36 140
MsG0580026166.01.T01 MTR_5g074800 31.378 392 162 18 52 392 1 336 2.89e-34 130
MsG0580026166.01.T01 MTR_8g033140 27.542 472 206 15 4 401 10 419 3.10e-34 132
MsG0580026166.01.T01 MTR_7g016930 31.436 369 154 11 5 341 9 310 1.15e-33 128
MsG0580026166.01.T01 MTR_7g023800 28.255 361 177 13 52 390 1 301 5.67e-33 126
MsG0580026166.01.T01 MTR_6g079790 29.545 308 144 8 130 401 40 310 2.30e-32 124
MsG0580026166.01.T01 MTR_5g075370 27.397 438 189 19 10 380 5 380 2.52e-30 123
MsG0580026166.01.T01 MTR_5g064700 27.628 409 193 16 52 399 1 367 5.58e-30 119
MsG0580026166.01.T01 MTR_5g092860 27.047 403 170 12 31 379 20 352 6.30e-30 119
MsG0580026166.01.T01 MTR_7g104710 37.109 256 127 12 5 237 10 254 8.05e-30 117
MsG0580026166.01.T01 MTR_5g094350 34.545 275 117 11 12 236 12 273 1.48e-29 116
MsG0580026166.01.T01 MTR_5g068490 33.775 302 130 12 114 401 33 278 2.65e-29 115
MsG0580026166.01.T01 MTR_5g037190 37.288 236 123 9 13 224 16 250 2.68e-29 114
MsG0580026166.01.T01 MTR_8g073570 44.578 166 81 4 12 170 9 170 3.54e-29 112
MsG0580026166.01.T01 MTR_5g097820 34.538 249 124 8 12 222 22 269 9.15e-29 113
MsG0580026166.01.T01 MTR_3g030620 36.216 185 73 4 197 379 19 160 1.35e-28 110
MsG0580026166.01.T01 MTR_5g092850 27.193 456 207 17 5 398 8 400 2.33e-28 115
MsG0580026166.01.T01 MTR_5g096900 34.173 278 135 10 1 237 1 271 2.41e-28 114
MsG0580026166.01.T01 MTR_6g087620 29.537 281 126 8 130 390 51 279 2.66e-28 112
MsG0580026166.01.T01 MTR_6g070880 40.816 196 85 9 6 178 25 212 3.37e-28 112
MsG0580026166.01.T01 MTR_6g071420 40.816 196 85 9 6 178 25 212 3.37e-28 112
MsG0580026166.01.T01 MTR_1g050308 42.021 188 92 5 1 177 23 204 1.48e-27 112
MsG0580026166.01.T01 MTR_1g050308 46.739 92 45 3 290 379 240 329 6.96e-13 69.7
MsG0580026166.01.T01 MTR_5g097650 41.135 141 73 4 8 145 17 150 4.91e-27 105
MsG0580026166.01.T01 MTR_6g090260 31.532 222 97 5 186 398 87 262 5.46e-27 108
MsG0580026166.01.T01 MTR_6g090260 63.636 55 20 0 5 59 12 66 1.55e-13 70.5
MsG0580026166.01.T01 MTR_1g050312 32.317 328 144 14 84 389 13 284 2.75e-26 107
MsG0580026166.01.T01 MTR_6g004450 31.408 277 101 9 157 399 47 268 5.10e-26 106
MsG0580026166.01.T01 MTR_6g079730 38.411 151 76 3 249 398 14 148 8.08e-26 102
MsG0580026166.01.T01 MTR_5g022850 26.807 429 170 14 1 382 5 336 1.06e-25 107
MsG0580026166.01.T01 MTR_1g076810 38.071 197 101 7 5 186 11 201 1.34e-25 109
MsG0580026166.01.T01 MTR_4g106530 53.061 98 24 1 141 238 4 79 8.22e-25 97.4
MsG0580026166.01.T01 MTR_6g465360 38.071 197 96 8 1 176 51 242 2.44e-24 102
MsG0580026166.01.T01 MTR_3g047580 42.553 141 59 5 1 137 1 123 5.01e-24 96.7
MsG0580026166.01.T01 MTR_5g086590 28.889 270 115 8 128 372 14 231 5.88e-22 94.4
MsG0580026166.01.T01 MTR_8g033000 35.176 199 87 5 198 396 203 359 6.92e-22 96.3
MsG0580026166.01.T01 MTR_5g066420 43.617 94 53 0 296 389 56 149 7.74e-22 91.3
MsG0580026166.01.T01 MTR_5g076490 32.946 258 92 8 157 378 101 313 1.42e-21 95.1
MsG0580026166.01.T01 MTR_5g097850 32.301 226 104 6 167 383 44 229 1.99e-21 93.2
MsG0580026166.01.T01 MTR_5g096070 40.361 166 64 5 7 165 20 157 5.07e-21 89.4
MsG0580026166.01.T01 MTR_5g097660 42.308 104 59 1 296 398 15 118 9.94e-21 87.4
MsG0580026166.01.T01 MTR_2g021920 27.240 279 114 9 159 395 37 268 1.00e-20 91.7
MsG0580026166.01.T01 MTR_8g035690 32.751 229 99 6 173 399 127 302 1.08e-20 92.0
MsG0580026166.01.T01 MTR_5g023970 33.854 192 81 5 198 389 39 184 8.96e-20 87.0
MsG0580026166.01.T01 MTR_5g036250 40.000 105 62 1 296 400 8 111 3.58e-19 85.1
MsG0580026166.01.T01 MTR_3g018750 31.707 205 92 6 198 399 81 240 4.57e-19 86.3
MsG0580026166.01.T01 MTR_1g097560 25.063 395 215 17 2 377 25 357 1.19e-18 87.0
MsG0580026166.01.T01 MTR_5g098950 29.741 232 110 7 130 357 91 273 2.08e-18 85.1
MsG0580026166.01.T01 MTR_6g009350 30.165 242 76 8 160 363 9 195 1.01e-17 81.3
MsG0580026166.01.T01 MTR_2g022020 38.947 95 56 1 296 390 18 110 1.03e-17 79.0
MsG0580026166.01.T01 MTR_5g096920 30.366 191 83 4 201 390 1 142 2.77e-17 79.0
MsG0580026166.01.T01 MTR_1g115205 33.047 233 116 11 1 207 17 235 3.20e-17 81.3
MsG0580026166.01.T01 MTR_6g087660 46.667 90 43 2 5 92 10 96 9.45e-17 78.6
MsG0580026166.01.T01 MTR_8g101580 25.905 359 211 14 21 368 54 368 9.70e-16 78.6
MsG0580026166.01.T01 MTR_8g041440 55.224 67 30 0 10 76 12 78 1.68e-15 72.8
MsG0580026166.01.T01 MTR_2g021150 25.070 359 214 15 12 356 13 330 2.06e-15 77.4
MsG0580026166.01.T01 MTR_2g104260 30.882 272 126 11 13 230 14 277 1.43e-14 73.9
MsG0580026166.01.T01 MTR_2g046920 22.021 386 212 14 12 368 23 348 1.82e-14 74.7
MsG0580026166.01.T01 MTR_5g086780 51.136 88 40 3 1 88 5 89 3.47e-14 72.0
MsG0580026166.01.T01 MTR_4g133170 23.834 386 223 16 16 381 7 341 8.81e-14 72.8
MsG0580026166.01.T01 MTR_3g014540 21.392 388 211 14 13 372 29 350 4.09e-13 70.5
MsG0580026166.01.T01 MTR_3g007410 26.220 328 180 15 21 335 60 338 7.99e-13 69.7
MsG0580026166.01.T01 MTR_4g107820 23.316 386 202 14 15 370 38 359 1.02e-12 69.3
MsG0580026166.01.T01 MTR_4g107780 23.316 386 202 14 15 370 38 359 1.02e-12 69.3
MsG0580026166.01.T01 MTR_3g007360 25.915 328 181 14 21 335 60 338 1.05e-12 69.7
MsG0580026166.01.T01 MTR_4g107780 23.316 386 202 14 15 370 38 359 1.15e-12 69.3
MsG0580026166.01.T01 MTR_4g107820 23.316 386 202 14 15 370 38 359 1.17e-12 69.3
MsG0580026166.01.T01 MTR_1g094210 22.251 391 206 16 13 374 57 378 1.17e-12 69.3
MsG0580026166.01.T01 MTR_1g097490 22.812 377 193 13 21 368 57 364 1.75e-12 68.9
MsG0580026166.01.T01 MTR_5g097210 22.651 415 221 14 7 389 3 349 1.98e-12 68.6
MsG0580026166.01.T01 MTR_3g436150 26.667 285 150 10 16 250 35 310 2.00e-12 68.6
MsG0580026166.01.T01 MTR_5g097540 29.121 182 75 5 181 357 100 232 2.87e-12 67.4
MsG0580026166.01.T01 MTR_4g094400 31.429 140 57 3 198 333 7 111 6.69e-12 62.8
MsG0580026166.01.T01 MTR_8g467140 25.194 258 146 10 7 234 26 266 1.15e-11 65.9
MsG0580026166.01.T01 MTR_1g094210 22.280 386 202 16 15 371 59 375 1.28e-11 66.2
MsG0580026166.01.T01 MTR_4g094365 24.179 335 205 12 11 309 6 327 2.43e-11 65.1
MsG0580026166.01.T01 MTR_3g013740 24.311 399 239 15 10 384 41 400 2.72e-11 65.1
MsG0580026166.01.T01 MTR_2g047832 23.316 386 227 17 12 368 28 373 3.03e-11 65.1
MsG0580026166.01.T01 MTR_1g047080 31.797 217 104 12 50 236 1 203 5.00e-11 63.5
MsG0580026166.01.T01 MTR_7g056003 24.627 268 151 10 4 228 6 265 5.71e-11 64.3
MsG0580026166.01.T01 MTR_3g013770 20.874 412 225 12 16 388 36 385 7.46e-11 63.9
MsG0580026166.01.T01 MTR_2g046830 24.691 243 154 8 12 234 24 257 8.47e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026166.01.T01 AT3G16210 22.407 241 153 9 10 235 2 223 3.82e-11 64.7

Find 83 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTACTAACCTTGTCATTCTT+TGG 0.174229 5:-31733419 None:intergenic
ATCATGAATGGGTTAAATTA+TGG 0.211161 5:+31733783 MsG0580026166.01.T01:CDS
TTTGATTTGGTGGTGTTAAA+TGG 0.211868 5:+31734380 MsG0580026166.01.T01:CDS
TGGTTTAATCTTCATGGATA+TGG 0.220601 5:+31733875 MsG0580026166.01.T01:CDS
CAGAGATTTGAGGGGTAGTT+TGG 0.250732 5:-31733480 None:intergenic
ATTGTTTCATTTGTGATTAA+TGG 0.260718 5:+31734245 MsG0580026166.01.T01:CDS
AATGTTTGGTTTAATCTTCA+TGG 0.270559 5:+31733869 MsG0580026166.01.T01:CDS
AATACAACTAATTTCTTATT+CGG 0.270685 5:-31734691 None:intergenic
TTAAATAGTAAAACCTTTGC+AGG 0.270923 5:-31733845 None:intergenic
TACTGAAATAGTTGAAGATT+TGG 0.281164 5:+31734604 MsG0580026166.01.T01:CDS
TTTGCTTTGTGGCGAGAATA+AGG 0.314114 5:+31733676 MsG0580026166.01.T01:CDS
GTTGTGACTTTGCAATTTAT+CGG 0.314236 5:+31734648 MsG0580026166.01.T01:CDS
GTGGTATTTCCTTGATATTT+AGG 0.316653 5:-31733625 None:intergenic
TCCACTTTGTATTTGCTTTG+TGG 0.333104 5:+31733665 MsG0580026166.01.T01:CDS
CCTTTATTTCCAACTCCAAT+AGG 0.346909 5:-31734530 None:intergenic
ATACAAAGTGGAACGATCTT+GGG 0.368397 5:-31733654 None:intergenic
CCTATTGGAGTTGGAAATAA+AGG 0.373444 5:+31734530 MsG0580026166.01.T01:CDS
TAATTTCTTATTCGGCGAAA+TGG 0.377606 5:-31734683 None:intergenic
TTTACCCTTGGATATGCATT+TGG 0.385033 5:+31734196 MsG0580026166.01.T01:CDS
GTAGATATGCCTATTGGTAT+AGG 0.390629 5:+31734065 MsG0580026166.01.T01:intron
CTTGTCATTCTTTGGATCCA+TGG 0.393328 5:-31733411 None:intergenic
AATACAAAGTGGAACGATCT+TGG 0.393435 5:-31733655 None:intergenic
GATTTGGTAGATATGCCTAT+TGG 0.394687 5:+31734059 MsG0580026166.01.T01:intron
TCTCTGATGTTGATCATGAA+TGG 0.418703 5:+31733771 MsG0580026166.01.T01:CDS
GGACGTAAGGTGTATTTGAA+TGG 0.420834 5:+31734086 MsG0580026166.01.T01:CDS
AATTATGGAATCCAGCTACT+AGG 0.422633 5:+31733798 MsG0580026166.01.T01:CDS
GTTATTGTATTTGTGATGGG+TGG 0.422720 5:+31734312 MsG0580026166.01.T01:CDS
TTGATTTGGTGGTGTTAAAT+GGG 0.430558 5:+31734381 MsG0580026166.01.T01:CDS
AATTTCAATTTAGAGAATCA+TGG 0.430562 5:+31734465 MsG0580026166.01.T01:CDS
TGTGAAGAGAAACTTTGATT+TGG 0.435868 5:+31734367 MsG0580026166.01.T01:CDS
CTCTGATGTTGATCATGAAT+GGG 0.443132 5:+31733772 MsG0580026166.01.T01:CDS
TTTCTCTTCACACACTCATC+GGG 0.447595 5:-31734356 None:intergenic
TGAAGATTTGGATATAAAAG+AGG 0.449852 5:+31734616 MsG0580026166.01.T01:CDS
TACCTAATGATAATGTTGAT+TGG 0.450600 5:+31733957 MsG0580026166.01.T01:CDS
CTTTACTACACATTTACCCT+TGG 0.456486 5:+31734184 MsG0580026166.01.T01:CDS
TTACTTGTGCGTGTAAATCA+TGG 0.457092 5:+31733510 MsG0580026166.01.T01:CDS
TGATGGGTGGGTGAAGGTGA+AGG 0.463052 5:+31734325 MsG0580026166.01.T01:CDS
ACCCTTGGATATGCATTTGG+CGG 0.464115 5:+31734199 MsG0580026166.01.T01:CDS
CTCCAATCAACATTATCATT+AGG 0.469722 5:-31733959 None:intergenic
CTTGACCGTCCTATTGGAGT+TGG 0.470977 5:+31734521 MsG0580026166.01.T01:CDS
TTACATTTATACTCATATGT+TGG 0.474239 5:+31733721 MsG0580026166.01.T01:CDS
AAATCGTTTCAGAGATTTGA+GGG 0.481510 5:-31733489 None:intergenic
ACCTTACGTCCTATACCAAT+AGG 0.489675 5:-31734074 None:intergenic
CTCACATATTCCCTAGTAGC+TGG 0.495466 5:-31733809 None:intergenic
GAAGAGAAACTTTGATTTGG+TGG 0.499011 5:+31734370 MsG0580026166.01.T01:CDS
ACGTATCTTCCTAAATATCA+AGG 0.506250 5:+31733616 MsG0580026166.01.T01:CDS
GTTTCTCTTCACACACTCAT+CGG 0.509586 5:-31734357 None:intergenic
TATACTCATATGTTGGACTC+TGG 0.509778 5:+31733728 MsG0580026166.01.T01:CDS
CGATACTCCACACTTACCCT+TGG 0.511219 5:+31734277 MsG0580026166.01.T01:CDS
GCCTATTGGTATAGGACGTA+AGG 0.511914 5:+31734073 MsG0580026166.01.T01:CDS
AGTAAAACCTTTGCAGGGCT+AGG 0.512585 5:-31733839 None:intergenic
TTCATTTGTGATTAATGGTG+AGG 0.515381 5:+31734250 MsG0580026166.01.T01:CDS
ATTATGGAATCCAGCTACTA+GGG 0.520382 5:+31733799 MsG0580026166.01.T01:CDS
TGTTCCTCCTAGCCCTGCAA+AGG 0.520625 5:+31733832 MsG0580026166.01.T01:CDS
ATAACTATCTTGCATATCCA+AGG 0.523543 5:-31734294 None:intergenic
GTGGAACGATCTTGGGGATG+TGG 0.524574 5:-31733647 None:intergenic
GATAGTTATTGTATTTGTGA+TGG 0.527988 5:+31734308 MsG0580026166.01.T01:CDS
CATGGATATGGTTATGACTA+CGG 0.533221 5:+31733887 MsG0580026166.01.T01:CDS
ATTGATTAACTTTAACATGA+CGG 0.550606 5:-31733931 None:intergenic
ATAGTTATTGTATTTGTGAT+GGG 0.550673 5:+31734309 MsG0580026166.01.T01:CDS
TAAATCGTTTCAGAGATTTG+AGG 0.559075 5:-31733490 None:intergenic
ATTGCAAAGTCACAACAAAA+CGG 0.563637 5:-31734641 None:intergenic
TAAATAGTAAAACCTTTGCA+GGG 0.568372 5:-31733844 None:intergenic
TACAAAGTGGAACGATCTTG+GGG 0.568944 5:-31733653 None:intergenic
TATTTGTGATGGGTGGGTGA+AGG 0.571569 5:+31734319 MsG0580026166.01.T01:CDS
TTATTGTATTTGTGATGGGT+GGG 0.572795 5:+31734313 MsG0580026166.01.T01:CDS
GATGGGTGGGTGAAGGTGAA+GGG 0.579224 5:+31734326 MsG0580026166.01.T01:CDS
ATCCGCCAAATGCATATCCA+AGG 0.585712 5:-31734201 None:intergenic
TATTTCCAACTCCAATAGGA+CGG 0.590021 5:-31734526 None:intergenic
TGCATATCCAAGGGTAAGTG+TGG 0.592323 5:-31734284 None:intergenic
TAGTTTGGAGAGAATAATGA+AGG 0.596600 5:-31733465 None:intergenic
TGAGTATAAATGTAATCATG+TGG 0.606720 5:-31733713 None:intergenic
GCCACAAAGCAAATACAAAG+TGG 0.613200 5:-31733666 None:intergenic
TATCTGTATTAGTCAACCAA+TGG 0.616253 5:-31734115 None:intergenic
AAAACCTTTGCAGGGCTAGG+AGG 0.620774 5:-31733836 None:intergenic
CATGGATCCAAAGAATGACA+AGG 0.633569 5:+31733412 None:intergenic
TAACTATCTTGCATATCCAA+GGG 0.636760 5:-31734293 None:intergenic
GAACGATCTTGGGGATGTGG+TGG 0.641442 5:-31733644 None:intergenic
ATTGGAGTTGGAAATAAAGG+TGG 0.645769 5:+31734533 MsG0580026166.01.T01:CDS
AGATAGAGATGATACATACA+TGG 0.649385 5:+31734133 MsG0580026166.01.T01:CDS
TCCGCCAAATGCATATCCAA+GGG 0.651039 5:-31734200 None:intergenic
AATCGTTTCAGAGATTTGAG+GGG 0.685519 5:-31733488 None:intergenic
TTATTCGGCGAAATGGACGA+CGG 0.700660 5:-31734676 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTACTATTTAATAATGTT+TGG + Chr5:31733855-31733874 MsG0580026166.01.T01:CDS 10.0%
!! AATTTCAATTTAGAGAATCA+TGG + Chr5:31734465-31734484 MsG0580026166.01.T01:CDS 20.0%
!! ATAGTTATTGTATTTGTGAT+GGG + Chr5:31734309-31734328 MsG0580026166.01.T01:CDS 20.0%
!! ATTGATTAACTTTAACATGA+CGG - Chr5:31733934-31733953 None:intergenic 20.0%
!! ATTGTTTCATTTGTGATTAA+TGG + Chr5:31734245-31734264 MsG0580026166.01.T01:CDS 20.0%
!! TTACATTTATACTCATATGT+TGG + Chr5:31733721-31733740 MsG0580026166.01.T01:CDS 20.0%
!!! CGAAACATTTTCATAAAATT+GGG - Chr5:31733554-31733573 None:intergenic 20.0%
!!! GAAACATTTTCATAAAATTG+GGG - Chr5:31733553-31733572 None:intergenic 20.0%
!!! TATTTTCACATATACGTTTT+GGG + Chr5:31734440-31734459 MsG0580026166.01.T01:CDS 20.0%
!!! TTATTTTCACATATACGTTT+TGG + Chr5:31734439-31734458 MsG0580026166.01.T01:CDS 20.0%
! GATAGTTATTGTATTTGTGA+TGG + Chr5:31734308-31734327 MsG0580026166.01.T01:CDS 25.0%
! TAAATAGTAAAACCTTTGCA+GGG - Chr5:31733847-31733866 None:intergenic 25.0%
! TACTGAAATAGTTGAAGATT+TGG + Chr5:31734604-31734623 MsG0580026166.01.T01:CDS 25.0%
! TGAAGATTTGGATATAAAAG+AGG + Chr5:31734616-31734635 MsG0580026166.01.T01:CDS 25.0%
! TGAGTATAAATGTAATCATG+TGG - Chr5:31733716-31733735 None:intergenic 25.0%
! TTAAATAGTAAAACCTTTGC+AGG - Chr5:31733848-31733867 None:intergenic 25.0%
!! AATGTTTGGTTTAATCTTCA+TGG + Chr5:31733869-31733888 MsG0580026166.01.T01:CDS 25.0%
!! GCGAAACATTTTCATAAAAT+TGG - Chr5:31733555-31733574 None:intergenic 25.0%
!! TACCTAATGATAATGTTGAT+TGG + Chr5:31733957-31733976 MsG0580026166.01.T01:CDS 25.0%
!!! ATCATGAATGGGTTAAATTA+TGG + Chr5:31733783-31733802 MsG0580026166.01.T01:CDS 25.0%
AAATCGTTTCAGAGATTTGA+GGG - Chr5:31733492-31733511 None:intergenic 30.0%
ACGTATCTTCCTAAATATCA+AGG + Chr5:31733616-31733635 MsG0580026166.01.T01:CDS 30.0%
AGATAGAGATGATACATACA+TGG + Chr5:31734133-31734152 MsG0580026166.01.T01:CDS 30.0%
ATAACTATCTTGCATATCCA+AGG - Chr5:31734297-31734316 None:intergenic 30.0%
ATTGCAAAGTCACAACAAAA+CGG - Chr5:31734644-31734663 None:intergenic 30.0%
CTCCAATCAACATTATCATT+AGG - Chr5:31733962-31733981 None:intergenic 30.0%
GTGGTATTTCCTTGATATTT+AGG - Chr5:31733628-31733647 None:intergenic 30.0%
GTTGTGACTTTGCAATTTAT+CGG + Chr5:31734648-31734667 MsG0580026166.01.T01:CDS 30.0%
TAAATCGTTTCAGAGATTTG+AGG - Chr5:31733493-31733512 None:intergenic 30.0%
TAACTATCTTGCATATCCAA+GGG - Chr5:31734296-31734315 None:intergenic 30.0%
TAATTTCTTATTCGGCGAAA+TGG - Chr5:31734686-31734705 None:intergenic 30.0%
TAGTTTGGAGAGAATAATGA+AGG - Chr5:31733468-31733487 None:intergenic 30.0%
TATCTGTATTAGTCAACCAA+TGG - Chr5:31734118-31734137 None:intergenic 30.0%
TGGTTTAATCTTCATGGATA+TGG + Chr5:31733875-31733894 MsG0580026166.01.T01:CDS 30.0%
TGTGAAGAGAAACTTTGATT+TGG + Chr5:31734367-31734386 MsG0580026166.01.T01:CDS 30.0%
TTCATTTGTGATTAATGGTG+AGG + Chr5:31734250-31734269 MsG0580026166.01.T01:CDS 30.0%
!! ATTTGAATGGATTTTGCCAT+TGG + Chr5:31734099-31734118 MsG0580026166.01.T01:CDS 30.0%
!! TTATTGTATTTGTGATGGGT+GGG + Chr5:31734313-31734332 MsG0580026166.01.T01:CDS 30.0%
!! TTGATTTGGTGGTGTTAAAT+GGG + Chr5:31734381-31734400 MsG0580026166.01.T01:CDS 30.0%
!! TTTGATTTGGTGGTGTTAAA+TGG + Chr5:31734380-31734399 MsG0580026166.01.T01:CDS 30.0%
AAGAAGTAACTCTTGGAAGT+TGG + Chr5:31734027-31734046 MsG0580026166.01.T01:intron 35.0%
AAGTTGGAAGAGACTTGATT+TGG + Chr5:31734043-31734062 MsG0580026166.01.T01:intron 35.0%
AATACAAAGTGGAACGATCT+TGG - Chr5:31733658-31733677 None:intergenic 35.0%
AATCGTTTCAGAGATTTGAG+GGG - Chr5:31733491-31733510 None:intergenic 35.0%
AATTATGGAATCCAGCTACT+AGG + Chr5:31733798-31733817 MsG0580026166.01.T01:CDS 35.0%
ATACAAAGTGGAACGATCTT+GGG - Chr5:31733657-31733676 None:intergenic 35.0%
ATAGCGTAAGAAGTAACTCT+TGG + Chr5:31734020-31734039 MsG0580026166.01.T01:intron 35.0%
ATTATGGAATCCAGCTACTA+GGG + Chr5:31733799-31733818 MsG0580026166.01.T01:CDS 35.0%
ATTGGAGTTGGAAATAAAGG+TGG + Chr5:31734533-31734552 MsG0580026166.01.T01:CDS 35.0%
CATGGATATGGTTATGACTA+CGG + Chr5:31733887-31733906 MsG0580026166.01.T01:CDS 35.0%
CCTATTGGAGTTGGAAATAA+AGG + Chr5:31734530-31734549 MsG0580026166.01.T01:CDS 35.0%
CTACTAACCTTGTCATTCTT+TGG - Chr5:31733422-31733441 None:intergenic 35.0%
CTCTGATGTTGATCATGAAT+GGG + Chr5:31733772-31733791 MsG0580026166.01.T01:CDS 35.0%
CTTTACTACACATTTACCCT+TGG + Chr5:31734184-31734203 MsG0580026166.01.T01:CDS 35.0%
GTAGATATGCCTATTGGTAT+AGG + Chr5:31734065-31734084 MsG0580026166.01.T01:intron 35.0%
TATACTCATATGTTGGACTC+TGG + Chr5:31733728-31733747 MsG0580026166.01.T01:CDS 35.0%
TATTTCCAACTCCAATAGGA+CGG - Chr5:31734529-31734548 None:intergenic 35.0%
TCCACTTTGTATTTGCTTTG+TGG + Chr5:31733665-31733684 MsG0580026166.01.T01:CDS 35.0%
TCTCTGATGTTGATCATGAA+TGG + Chr5:31733771-31733790 MsG0580026166.01.T01:CDS 35.0%
TTACTTGTGCGTGTAAATCA+TGG + Chr5:31733510-31733529 MsG0580026166.01.T01:CDS 35.0%
TTTACCCTTGGATATGCATT+TGG + Chr5:31734196-31734215 MsG0580026166.01.T01:CDS 35.0%
! CCTTTATTTCCAACTCCAAT+AGG - Chr5:31734533-31734552 None:intergenic 35.0%
! GATTTGGTAGATATGCCTAT+TGG + Chr5:31734059-31734078 MsG0580026166.01.T01:intron 35.0%
! GTTATTGTATTTGTGATGGG+TGG + Chr5:31734312-31734331 MsG0580026166.01.T01:CDS 35.0%
!! GAAGAGAAACTTTGATTTGG+TGG + Chr5:31734370-31734389 MsG0580026166.01.T01:CDS 35.0%
!!! TTCTTTTGACTTGAGTAGTG+AGG + Chr5:31734157-31734176 MsG0580026166.01.T01:CDS 35.0%
ACCTTACGTCCTATACCAAT+AGG - Chr5:31734077-31734096 None:intergenic 40.0%
ACGCTATAAATCTCCCAGAA+AGG - Chr5:31734007-31734026 None:intergenic 40.0%
AGGACGGTCAAGACAAAAAA+AGG - Chr5:31734513-31734532 None:intergenic 40.0%
CGCTATAAATCTCCCAGAAA+GGG - Chr5:31734006-31734025 None:intergenic 40.0%
CGGTCAAGACAAAAAAAGGA+TGG - Chr5:31734509-31734528 None:intergenic 40.0%
GCCACAAAGCAAATACAAAG+TGG - Chr5:31733669-31733688 None:intergenic 40.0%
GGACGTAAGGTGTATTTGAA+TGG + Chr5:31734086-31734105 MsG0580026166.01.T01:CDS 40.0%
GTTTCTCTTCACACACTCAT+CGG - Chr5:31734360-31734379 None:intergenic 40.0%
TACAAAGTGGAACGATCTTG+GGG - Chr5:31733656-31733675 None:intergenic 40.0%
TTTCTCTTCACACACTCATC+GGG - Chr5:31734359-31734378 None:intergenic 40.0%
TTTGCTTTGTGGCGAGAATA+AGG + Chr5:31733676-31733695 MsG0580026166.01.T01:CDS 40.0%
! TTTTGTCTTGACCGTCCTAT+TGG + Chr5:31734515-31734534 MsG0580026166.01.T01:CDS 40.0%
ACCCTTGGATATGCATTTGG+CGG + Chr5:31734199-31734218 MsG0580026166.01.T01:CDS 45.0%
AGTAAAACCTTTGCAGGGCT+AGG - Chr5:31733842-31733861 None:intergenic 45.0%
ATCCGCCAAATGCATATCCA+AGG - Chr5:31734204-31734223 None:intergenic 45.0%
ATTGACACCACACCCTTTCT+GGG + Chr5:31733991-31734010 MsG0580026166.01.T01:intron 45.0%
CAGAGATTTGAGGGGTAGTT+TGG - Chr5:31733483-31733502 None:intergenic 45.0%
CTCACATATTCCCTAGTAGC+TGG - Chr5:31733812-31733831 None:intergenic 45.0%
GCCTATTGGTATAGGACGTA+AGG + Chr5:31734073-31734092 MsG0580026166.01.T01:CDS 45.0%
TAAATCTCCCAGAAAGGGTG+TGG - Chr5:31734001-31734020 None:intergenic 45.0%
TCCGCCAAATGCATATCCAA+GGG - Chr5:31734203-31734222 None:intergenic 45.0%
TGCATATCCAAGGGTAAGTG+TGG - Chr5:31734287-31734306 None:intergenic 45.0%
TTATTCGGCGAAATGGACGA+CGG - Chr5:31734679-31734698 None:intergenic 45.0%
!! TATTTGTGATGGGTGGGTGA+AGG + Chr5:31734319-31734338 MsG0580026166.01.T01:CDS 45.0%
AAAACCTTTGCAGGGCTAGG+AGG - Chr5:31733839-31733858 None:intergenic 50.0%
CATTGACACCACACCCTTTC+TGG + Chr5:31733990-31734009 MsG0580026166.01.T01:intron 50.0%
CGATACTCCACACTTACCCT+TGG + Chr5:31734277-31734296 MsG0580026166.01.T01:CDS 50.0%
CTTGACCGTCCTATTGGAGT+TGG + Chr5:31734521-31734540 MsG0580026166.01.T01:CDS 50.0%
! CAGAAAGGGTGTGGTGTCAA+TGG - Chr5:31733992-31734011 None:intergenic 50.0%
GAACGATCTTGGGGATGTGG+TGG - Chr5:31733647-31733666 None:intergenic 55.0%
GATGGGTGGGTGAAGGTGAA+GGG + Chr5:31734326-31734345 MsG0580026166.01.T01:CDS 55.0%
GTGGAACGATCTTGGGGATG+TGG - Chr5:31733650-31733669 None:intergenic 55.0%
TGTTCCTCCTAGCCCTGCAA+AGG + Chr5:31733832-31733851 MsG0580026166.01.T01:CDS 55.0%
! TGATGGGTGGGTGAAGGTGA+AGG + Chr5:31734325-31734344 MsG0580026166.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr5 gene 31733413 31734706 31733413 ID=MsG0580026166.01;Name=MsG0580026166.01
Chr5 mRNA 31733413 31734706 31733413 ID=MsG0580026166.01.T01;Parent=MsG0580026166.01;Name=MsG0580026166.01.T01;_AED=0.43;_eAED=0.43;_QI=0|0|0|1|1|1|2|0|401
Chr5 exon 31733413 31733980 31733413 ID=MsG0580026166.01.T01:exon:13149;Parent=MsG0580026166.01.T01
Chr5 exon 31734069 31734706 31734069 ID=MsG0580026166.01.T01:exon:13150;Parent=MsG0580026166.01.T01
Chr5 CDS 31733413 31733980 31733413 ID=MsG0580026166.01.T01:cds;Parent=MsG0580026166.01.T01
Chr5 CDS 31734069 31734706 31734069 ID=MsG0580026166.01.T01:cds;Parent=MsG0580026166.01.T01
Gene Sequence

>MsG0580026166.01.T01

ATGGATCCAAAGAATGACAAGGTTAGTAGTATCTATATTCACGACGATGTTTCCTTCATTATTCTCTCCAAACTACCCCTCAAATCTCTGAAACGATTTACTTGTGCGTGTAAATCATGGTCTCTTTTATTTGAAAACCCCAATTTTATGAAAATGTTTCGCAAAAAATTCATGTCTATGCATCGATCATTGTACGATGATACACGTATCTTCCTAAATATCAAGGAAATACCACCACATCCCCAAGATCGTTCCACTTTGTATTTGCTTTGTGGCGAGAATAAGGTCAATTCGACATGTCCACATGATTACATTTATACTCATATGTTGGACTCTGGTATTAATGATGTTCTGTGTTTCTCTGATGTTGATCATGAATGGGTTAAATTATGGAATCCAGCTACTAGGGAATATGTGAGTGTTCCTCCTAGCCCTGCAAAGGTTTTACTATTTAATAATGTTTGGTTTAATCTTCATGGATATGGTTATGACTACGGTAATGAAGACTATAAGATCATCCGTCATGTTAAAGTTAATCAATATATACCTAATGATAATGTTGATTGGAATATGCCTATTGGTATAGGACGTAAGGTGTATTTGAATGGATTTTGCCATTGGTTGACTAATACAGATAGAGATGATACATACATGGTTTCTTTTGACTTGAGTAGTGAGGTGTTCTTTACTACACATTTACCCTTGGATATGCATTTGGCGGATACAAATAGAGATGATGCATACATTGTTTCATTTGTGATTAATGGTGAGGTGTTCGATACTCCACACTTACCCTTGGATATGCAAGATAGTTATTGTATTTGTGATGGGTGGGTGAAGGTGAAGGGAGAAAGTCCCGATGAGTGTGTGAAGAGAAACTTTGATTTGGTGGTGTTAAATGGGTTTGTAGCTATGATTTCATATAATGTTAACACATATTATTTTCACATATACGTTTTGGGTGAATTTCAATTTAGAGAATCATGGACAAAACTTTTCATTGTGCCATCCTTTTTTTGTCTTGACCGTCCTATTGGAGTTGGAAATAAAGGTGGTATATTCTTTATGAAAGATGACAACAAACTAGCTTATTATGATTTAAATACTGAAATAGTTGAAGATTTGGATATAAAAGAGGAGCCGTTTTGTTGTGACTTTGCAATTTATCGGTTGAACCGTCGTCCATTTCGCCGAATAAGAAATTAG
Protein sequence

>MsG0580026166.01.T01

MDPKNDKVSSIYIHDDVSFIILSKLPLKSLKRFTCACKSWSLLFENPNFMKMFRKKFMSMHRSLYDDTRIFLNIKEIPPHPQDRSTLYLLCGENKVNSTCPHDYIYTHMLDSGINDVLCFSDVDHEWVKLWNPATREYVSVPPSPAKVLLFNNVWFNLHGYGYDYGNEDYKIIRHVKVNQYIPNDNVDWNMPIGIGRKVYLNGFCHWLTNTDRDDTYMVSFDLSSEVFFTTHLPLDMHLADTNRDDAYIVSFVINGEVFDTPHLPLDMQDSYCICDGWVKVKGESPDECVKRNFDLVVLNGFVAMISYNVNTYYFHIYVLGEFQFRESWTKLFIVPSFFCLDRPIGVGNKGGIFFMKDDNKLAYYDLNTEIVEDLDIKEEPFCCDFAIYRLNRRPFRRIRN*