Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028082.01.T01 | AES98425.1 | 81.919 | 271 | 49 | 0 | 87 | 357 | 8 | 278 | 6.30E-155 | 446 |
Co-expression Network
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028082.01.T01 | MTR_5g068490 | 81.919 | 271 | 49 | 0 | 87 | 357 | 8 | 278 | 7.63e-159 | 446 |
| MsG0580028082.01.T01 | MTR_5g043605 | 59.030 | 371 | 89 | 9 | 6 | 356 | 12 | 339 | 8.95e-134 | 386 |
| MsG0580028082.01.T01 | MTR_3g011120 | 52.000 | 375 | 144 | 14 | 1 | 345 | 1 | 369 | 9.63e-107 | 317 |
| MsG0580028082.01.T01 | MTR_1g098180 | 48.101 | 395 | 139 | 13 | 1 | 357 | 1 | 367 | 1.90e-103 | 309 |
| MsG0580028082.01.T01 | MTR_8g467670 | 47.558 | 389 | 163 | 13 | 1 | 357 | 1 | 380 | 1.24e-98 | 298 |
| MsG0580028082.01.T01 | MTR_5g024260 | 47.949 | 390 | 148 | 15 | 1 | 341 | 7 | 390 | 1.37e-94 | 288 |
| MsG0580028082.01.T01 | MTR_0831s0010 | 44.898 | 392 | 162 | 16 | 2 | 351 | 37 | 416 | 3.25e-94 | 287 |
| MsG0580028082.01.T01 | MTR_8g063440 | 43.915 | 378 | 177 | 10 | 13 | 357 | 9 | 384 | 5.40e-94 | 285 |
| MsG0580028082.01.T01 | MTR_2g066170 | 45.153 | 392 | 175 | 14 | 1 | 357 | 1 | 387 | 5.88e-93 | 283 |
| MsG0580028082.01.T01 | MTR_2g066200 | 45.570 | 395 | 171 | 15 | 1 | 357 | 1 | 389 | 1.22e-92 | 282 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028082.01.T01 | AT1G47390 | 28.692 | 237 | 145 | 10 | 24 | 248 | 15 | 239 | 2.39e-15 | 77.0 |
| MsG0580028082.01.T01 | AT3G16210 | 24.315 | 292 | 198 | 11 | 13 | 299 | 2 | 275 | 1.43e-14 | 74.3 |
| MsG0580028082.01.T01 | AT3G17500 | 24.281 | 313 | 219 | 8 | 19 | 322 | 8 | 311 | 1.77e-14 | 74.3 |
| MsG0580028082.01.T01 | AT3G17500 | 24.281 | 313 | 219 | 8 | 19 | 322 | 7 | 310 | 2.84e-14 | 73.9 |
| MsG0580028082.01.T01 | AT3G21410 | 26.298 | 289 | 178 | 12 | 24 | 293 | 46 | 318 | 4.09e-14 | 73.6 |
| MsG0580028082.01.T01 | AT3G22350 | 26.389 | 288 | 177 | 11 | 19 | 286 | 7 | 279 | 2.31e-13 | 70.9 |
| MsG0580028082.01.T01 | AT2G23160 | 25.506 | 247 | 155 | 8 | 11 | 232 | 2 | 244 | 3.07e-12 | 67.0 |
| MsG0580028082.01.T01 | AT3G22650 | 23.973 | 292 | 185 | 11 | 19 | 293 | 12 | 283 | 3.14e-12 | 67.4 |
| MsG0580028082.01.T01 | AT3G17620 | 25.000 | 312 | 197 | 11 | 25 | 309 | 14 | 315 | 3.54e-12 | 67.4 |
| MsG0580028082.01.T01 | AT3G22650 | 24.068 | 295 | 192 | 11 | 19 | 293 | 12 | 294 | 1.01e-11 | 66.2 |
Find 67 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATACAACTACAGTCTTGC+CGG | 0.416642 | 5:-74304817 | None:intergenic |
| AAATGATTCCTGGAGGAAAA+TGG | 0.298690 | 5:+74305013 | MsG0580028082.01.T01:CDS |
| AAATTGGCGTCGAGGAATCA+TGG | 0.448593 | 5:+74305300 | MsG0580028082.01.T01:CDS |
| AACGGGAAGAAGGTTAGTAA+TGG | 0.624120 | 5:+74304474 | MsG0580028082.01.T01:CDS |
| AACTCTTTCTACTTTCTTTC+TGG | 0.073703 | 5:+74304687 | MsG0580028082.01.T01:CDS |
| AAGCGCTTAATAGATTTGAG+AGG | 0.611953 | 5:-74304546 | None:intergenic |
| AATGGCATTCTTTGTATCAC+CGG | 0.498939 | 5:+74304798 | MsG0580028082.01.T01:CDS |
| AATGGGATATGTCATTGGTT+GGG | 0.549037 | 5:+74305080 | MsG0580028082.01.T01:CDS |
| AATTGGCGTCGAGGAATCAT+GGG | 0.582935 | 5:+74305301 | MsG0580028082.01.T01:CDS |
| ACCAATCTCCTCAATCACCC+CGG | 0.678951 | 5:-74305438 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACTCTTTCTACTTTCTTTC+TGG | + | Chr5:74304687-74304706 | MsG0580028082.01.T01:CDS | 30.0% | |
| AGAAAAGAATCATGATAGAG+TGG | - | Chr5:74304648-74304667 | None:intergenic | 30.0% | |
| AGACTATATATCTCCCAAAA+AGG | - | Chr5:74304990-74305009 | None:intergenic | 30.0% | |
| CAATAACTACAAATGGAAGA+AGG | - | Chr5:74305508-74305527 | None:intergenic | 30.0% | |
| GAAAGAAAGTAGAAAGAGTT+CGG | - | Chr5:74304687-74304706 | None:intergenic | 30.0% | |
| GACTATATATCTCCCAAAAA+GGG | - | Chr5:74304989-74305008 | None:intergenic | 30.0% | |
| TTAACCTGCAAGTGTTAAAT+GGG | + | Chr5:74305216-74305235 | MsG0580028082.01.T01:CDS | 30.0% | |
| AAATACAACTACAGTCTTGC+CGG | - | Chr5:74304820-74304839 | None:intergenic | 35.0% | |
| AAATGATTCCTGGAGGAAAA+TGG | + | Chr5:74305013-74305032 | MsG0580028082.01.T01:CDS | 35.0% | |
| AAGCGCTTAATAGATTTGAG+AGG | - | Chr5:74304549-74304568 | None:intergenic | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 74304462 | 74305535 | 74304462 | ID=MsG0580028082.01;Name=MsG0580028082.01 |
| Chr5 | mRNA | 74304462 | 74305535 | 74304462 | ID=MsG0580028082.01.T01;Parent=MsG0580028082.01;Name=MsG0580028082.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|357 |
| Chr5 | exon | 74304462 | 74305535 | 74304462 | ID=MsG0580028082.01.T01:exon:45630;Parent=MsG0580028082.01.T01 |
| Chr5 | CDS | 74304462 | 74305535 | 74304462 | ID=MsG0580028082.01.T01:cds;Parent=MsG0580028082.01.T01 |
| Gene Sequence |
>MsG0580028082.01.T01 ATGACTCCAACGAACGGGAAGAAGGTTAGTAATGGTAGTAACTTTGTTCACGATGACATTGCTTTGTGTATTCTTTCTAAACTTCCTCTCAAATCTATTAAGCGCTTCTCTTGTGTTCGTAAATCATGGTCTCTTTTATTTGAAAACCCTAATTTCATCAAAATGTTCATTTCCAATTCTCATCCACTCTATCATGATTCTTTTCTCATTTTAAATCAGTATCCGAACTCTTTCTACTTTCTTTCTGGTCACAAGTTTCAGAATAAACTCAAATTGAAATTTCCACCTCCATTTCTCGATCCACAGCTTACCATTAGTCGAATTATAGATTCTGATAATGGCATTCTTTGTATCACCGGCAAGACTGTAGTTGTATTTTGGAATCCTGCTACTGAGGAAGTTAAGGTCATTCCTCCTTCTCAACTTGAGTTTTCCTGTGGAGTTAAGGATCATGGATTTGGCTATGACCATGTTAGGGATGACTATAAGCTCATCCAATATGTGAATGTTGTGGAGGGTCATGACCCTTTTTGGGAGATATATAGTCTAAAAAATGATTCCTGGAGGAAAATGGATATTGATATGCCAATTTGTGTAAATGATGATAATGTGTGCTTGAATGGGATATGTCATTGGTTGGGTGAAGAAAATGATGACTATGATATGGTTCTTGTGTCATTTCACTTGGGCAATGAGGTGTGTTTTGTTACACCTTTACCCTTTTTAGAAGGCAAGGATTATGATTTTGATGATGTTAACCTGCAAGTGTTAAATGGGTCGGTTGCTGTGATATTTAATCATATAGAGACAAAGTCTTTTCACATATCAATCCTGGGTGAAATTGGCGTCGAGGAATCATGGGTTAGACTCTTTGATATTGGACCTTTGTCTTGCATTATGCGTCCTATCATAGTATGGAAGAAGGGCAGTATAATTGTTAGAAAATTTGATCAACTAGCTTGTTTTGATCTAACTACCGGGGTGATTGAGGAGATTGGTGTTCAAGCAGAACATTTTTGCGACATTTTCATTTACAAGAAAAACCTTCTTCCATTTGTAGTTATTGACAATTAA |
| Protein sequence |
>MsG0580028082.01.T01 MTPTNGKKVSNGSNFVHDDIALCILSKLPLKSIKRFSCVRKSWSLLFENPNFIKMFISNSHPLYHDSFLILNQYPNSFYFLSGHKFQNKLKLKFPPPFLDPQLTISRIIDSDNGILCITGKTVVVFWNPATEEVKVIPPSQLEFSCGVKDHGFGYDHVRDDYKLIQYVNVVEGHDPFWEIYSLKNDSWRKMDIDMPICVNDDNVCLNGICHWLGEENDDYDMVLVSFHLGNEVCFVTPLPFLEGKDYDFDDVNLQVLNGSVAVIFNHIETKSFHISILGEIGVEESWVRLFDIGPLSCIMRPIIVWKKGSIIVRKFDQLACFDLTTGVIEEIGVQAEHFCDIFIYKKNLLPFVVIDN* |
